FvH4_6g40230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008528
Physical Location & Seq
Reverse (-)
31754529 .. 31756954
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g40230.t1

Sequence Viewer

Length: 714 bp
ATGAAGCTAGGTCCAAAGGTAGTGCTTCTCTTGAAAGACCCAGAGGGCTTCGGCGAGGCCATCTCCGACGCATTTCACCCTAACCCAACTGGTTCCGTCTCAGAAGAAAGCTTCGAGCTTTCGCTGGAGCGCTATGGGATCCGGAACTGCAAAGCTTCTGGAAAAGTTCGTCACTTCCTTGATCAGCAGGGTCAATTTGAGGTGTCAGTGTTGCTTATGGAATATTATGAATCTCCGATACTAGCATGTGCTTTTAATGAAGTTCTGGCTCAAATAGCAGGACTAAAATCATCCTCAATCCCCACAATTGTTGCCCCCTTTTTTGTTGCATCATCCAAGCTAAAATGGGACAGTAAATCTGCAACAAAGGTTGACAGCAAAGGTTCACTTTATGGTATACAGATAGGTGCAGAAACAGACATAAGCAAGGCCATCATTGCTCGAACCCAGAAAGCACCACCGACATTACAGATTCATTATGAACCTCTAGCCTGCCTTCTTCAGTTGGTCCGTGTCTTGAATTTGCCAACTTTTATTCTCATAGGGCAAAGGGATCAGAGGATTTCTGATAAAGAAGAGCTTGAGATACTTTATGAAATTGGGGAACTTTTGGCAAACACTTGTCAGGTAAACTTCTCAAGAGGCAAAATCACATGGAAGCCAACAAAAAAATCGAAGGATGAACAGGAGCCATGGCGTGCATTGTATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

26.38

Weight (kDa)

6.84

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7894 PF25428 1 - 237 8.6e-108 Domain of unknown function (DUF7894)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 397
AccIII TCCGGA 1 cut(s) 141
AclWI GGATC 3 cut(s) 133, 146, 561
AcsI RAATTY 1 cut(s) 520
AcuI CTGAAG 1 cut(s) 485
AfeI AGCGCT 1 cut(s) 131
AgsI TTSAA 2 cut(s) 34, 520
AluBI AGCT 6 cut(s) 7, 111, 118, 155, 340, 580
AluI AGCT 6 cut(s) 7, 111, 118, 155, 340, 580
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 3 cut(s) 133, 146, 561
Aor13HI TCCGGA 1 cut(s) 141
Aor51HI AGCGCT 1 cut(s) 131
AoxI GGCC 2 cut(s) 57, 429
ApoI RAATTY 1 cut(s) 520
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 2 cut(s) 11, 508
AsuHPI GGTGA 1 cut(s) 68
AvaII GGWCC 2 cut(s) 11, 508
BamHI GGATCC 1 cut(s) 138
BccI CCATC 2 cut(s) 68, 440
BclI TGATCA 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 103
BfaI CTAG 3 cut(s) 8, 242, 488
BfoI RGCGCY 1 cut(s) 133
Bme18I GGWCC 2 cut(s) 11, 508
BmgT120I GGNCC 2 cut(s) 11, 508
BmiI GGNNCC 3 cut(s) 94, 140, 690
BmsI GCATC 1 cut(s) 338
BplI GAGNNNNNCTC 2 cut(s) 47, 79
BpmI CTGGAG 1 cut(s) 146
BpuEI CTTGAG 2 cut(s) 602, 622
BsaJI CCNNGG 1 cut(s) 692
BsaWI WCCGGW 1 cut(s) 141
Bse1I ACTGG 1 cut(s) 94
Bse3DI GCAATG 1 cut(s) 435
BseAI TCCGGA 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 692
BseGI GGATG 3 cut(s) 290, 332, 685
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 94
BsgI GTGCAG 1 cut(s) 429
BshFI GGCC 2 cut(s) 59, 431
BsiSI CCGG 1 cut(s) 142
BslFI GGGAC 1 cut(s) 362
BsmAI GTCTC 1 cut(s) 103
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 2 cut(s) 59, 431
Bsp13I TCCGGA 1 cut(s) 141
Bsp143I GATC 3 cut(s) 138, 181, 553
Bsp19I CCATGG 1 cut(s) 692
BspANI GGCC 2 cut(s) 59, 431
BspCNI CTCAG 1 cut(s) 113
BspEI TCCGGA 1 cut(s) 141
BspLI GGNNCC 3 cut(s) 94, 140, 690
BspPI GGATC 3 cut(s) 133, 146, 561
BspQI GCTCTTC 1 cut(s) 570
BsrDI GCAATG 1 cut(s) 435
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 1 cut(s) 692
BssMI GATC 3 cut(s) 138, 181, 553
BssNAI GTATAC 1 cut(s) 398
BssT1I CCWWGG 1 cut(s) 692
Bst1107I GTATAC 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 353
Bst6I CTCTTC 1 cut(s) 570
BstC8I GCNNGC 2 cut(s) 493, 699
BstDEI CTNAG 1 cut(s) 100
BstDSI CCRYGG 1 cut(s) 692
BstF5I GGATG 3 cut(s) 290, 332, 685
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 3 cut(s) 141, 184, 556
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 3 cut(s) 138, 181, 553
BstMWI GCNNNNNNNGC 2 cut(s) 275, 437
BstNSI RCATGY 1 cut(s) 249
BstX2I RGATCY 1 cut(s) 138
BstYI RGATCY 1 cut(s) 138
BstZ17I GTATAC 1 cut(s) 398
BsuRI GGCC 2 cut(s) 59, 431
BtgI CCRYGG 1 cut(s) 692
BtsCI GGATG 3 cut(s) 290, 332, 685
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 493, 699
CfoI GCGC 1 cut(s) 132
Cfr13I GGNCC 2 cut(s) 11, 508
CseI GACGC 1 cut(s) 77
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 3 cut(s) 246, 654, 693
DdeI CTNAG 1 cut(s) 100
DpnI GATC 3 cut(s) 140, 183, 555
DpnII GATC 3 cut(s) 138, 181, 553
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco130I CCWWGG 1 cut(s) 692
Eco47I GGWCC 2 cut(s) 11, 508
Eco47III AGCGCT 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 485
EcoT14I CCWWGG 1 cut(s) 692
ErhI CCWWGG 1 cut(s) 692
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 3 cut(s) 249, 657, 696
FalI AAGNNNNNCTT 4 cut(s) 372, 404, 564, 596
FaqI GGGAC 1 cut(s) 362
FatI CATG 3 cut(s) 245, 653, 692
FbaI TGATCA 1 cut(s) 181
FblI GTMKAC 1 cut(s) 397
FokI GGATG 3 cut(s) 277, 319, 692
FspBI CTAG 3 cut(s) 8, 242, 488
GlaI GCGC 1 cut(s) 131
GsuI CTGGAG 1 cut(s) 146
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 2 cut(s) 59, 431
HapII CCGG 1 cut(s) 142
HgaI GACGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 132
Hin1II CATG 3 cut(s) 249, 657, 696
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 2 cut(s) 109, 153
HinfI GANTC 2 cut(s) 230, 472
HpaII CCGG 1 cut(s) 142
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 4 cut(s) 373, 386, 398, 631
Hpy188I TCNGA 5 cut(s) 67, 103, 237, 558, 568
Hpy188III TCNNGA 5 cut(s) 31, 142, 159, 517, 639
Hpy8I GTNNAC 4 cut(s) 373, 386, 398, 631
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 2 cut(s) 506, 670
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4V TGCA 5 cut(s) 150, 329, 362, 410, 701
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 437
HpyF3I CTNAG 1 cut(s) 100
Hsp92II CATG 3 cut(s) 249, 657, 696
HspAI GCGC 1 cut(s) 130
Kpn2I TCCGGA 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 3 cut(s) 138, 181, 553
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 2 cut(s) 127, 688
LweI GCATC 1 cut(s) 338
MaeI CTAG 3 cut(s) 8, 242, 488
MaeIII GTNAC 1 cut(s) 170
MalI GATC 3 cut(s) 140, 183, 555
MboI GATC 3 cut(s) 138, 181, 553
MboII GAAGA 3 cut(s) 116, 491, 587
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 1 cut(s) 138
MluCI AATT 4 cut(s) 194, 306, 520, 597
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 7 cut(s) 37, 49, 193, 304, 495, 552, 635
MroI TCCGGA 1 cut(s) 141
MseI TTAA 1 cut(s) 255
MspI CCGG 1 cut(s) 142
MunI CAATTG 1 cut(s) 306
MwoI GCNNNNNNNGC 2 cut(s) 275, 437
NcoI CCATGG 1 cut(s) 692
NdeII GATC 3 cut(s) 138, 181, 553
NlaIII CATG 3 cut(s) 249, 657, 696
NlaIV GGNNCC 3 cut(s) 94, 140, 690
NmuCI GTSAC 1 cut(s) 170
NspI RCATGY 1 cut(s) 249
PciSI GCTCTTC 1 cut(s) 570
PfeI GAWTC 2 cut(s) 230, 472
PspN4I GGNNCC 3 cut(s) 94, 140, 690
PspPI GGNCC 2 cut(s) 11, 508
PsuI RGATCY 1 cut(s) 138
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 1 cut(s) 255
Sau3AI GATC 3 cut(s) 138, 181, 553
Sau96I GGNCC 2 cut(s) 11, 508
SfaNI GCATC 1 cut(s) 338
SinI GGWCC 2 cut(s) 11, 508
SmlI CTYRAG 2 cut(s) 581, 637
SmoI CTYRAG 2 cut(s) 581, 637
Sse9I AATT 4 cut(s) 194, 306, 520, 597
SspI AATATT 1 cut(s) 224
SspMI CTAG 3 cut(s) 8, 242, 488
StyI CCWWGG 1 cut(s) 692
TaaI ACNGT 1 cut(s) 353
TaqI TCGA 3 cut(s) 114, 442, 674
TasI AATT 4 cut(s) 194, 306, 520, 597
TfiI GAWTC 2 cut(s) 230, 472
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 1 cut(s) 213
TseFI GTSAC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 7 cut(s) 17, 243, 273, 464, 495, 609, 696
TspGWI ACGGA 2 cut(s) 85, 500
TspRI CASTG 1 cut(s) 213
VpaK11BI GGWCC 2 cut(s) 11, 508
XapI RAATTY 1 cut(s) 520
XceI RCATGY 1 cut(s) 249
XmiI GTMKAC 1 cut(s) 397
XspI CTAG 3 cut(s) 8, 242, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.