Rh2AG512800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008528
Physical Location & Seq
Reverse (-)
74049598 .. 74052074
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG512800.1

Sequence Viewer

Length: 684 bp
ATGGAGGTAGGTCCAAAGGTAGTGCTTCTGTTGAGAGACCCAGAGGGCTTCGGCCAAGCCATCTCCGACGCATTCCACCCTAACCCAACTGCTTCCGTCACAGAGGAAAGCTTCGAGCTTTCGCTGGAGCGCTATGGGATCCGAAATTGCAAAGCTTCTGGAAAAGTTCGTCACTTTCTTGATCAGCACGGTCAATATGAGGTGTCAGTGTTGCTTATGGAATATTATGAATCACCGATACTAGCATGTGCTTTTAATGAAGTTCTGGCTGAACTAGCAGGACAAAAATCATCCTCAATGCCCACCATTGTTGCCCCCTTTTTCGTTGCATCATCCAAGCTTAAATGGGACAGTAAATCTGCAACAAAGTTTGACAGCAAAGGTTCACTTTATGGTATACAGATAGGTCCAGAAACAGACATAAGCAAGGCCATCATTGCCAGAACCCAGAAAGCACCACCGTCATTACAGATTCATCATGAACCTCTAGCCTGCCTTCTTCAGTTGGTCCGTGTCCTGAATTTGGCAACTCTTGTTCTTATAGGGCAAAGGGGTCAGCGAATTTCTGATAAAGAAGAGCTTGAGGTGACCATTTTTTTTATTTACTTTTTAGCTAATGCAACTCTCAAATTCAGTTCACTTCAAAATTGTGGTGTGATTTGGGAAAATTCAAGTTATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.16

Weight (kDa)

5.98

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7894 PF25428 1 - 212 3.6e-89 Domain of unknown function (DUF7894)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 397
AclWI GGATC 2 cut(s) 133, 146
AcoI YGGCCR 1 cut(s) 52
AcsI RAATTY 4 cut(s) 520, 561, 629, 667
AcuI CTGAAG 1 cut(s) 485
AfeI AGCGCT 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 523
AgsI TTSAA 2 cut(s) 644, 672
AluBI AGCT 6 cut(s) 111, 118, 155, 340, 580, 614
AluI AGCT 6 cut(s) 111, 118, 155, 340, 580, 614
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 2 cut(s) 133, 146
Aor51HI AGCGCT 1 cut(s) 131
AoxI GGCC 2 cut(s) 52, 429
ApoI RAATTY 4 cut(s) 520, 561, 629, 667
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 3 cut(s) 11, 407, 508
AsuHPI GGTGA 2 cut(s) 225, 598
AvaII GGWCC 3 cut(s) 11, 407, 508
BamHI GGATCC 1 cut(s) 138
BccI CCATC 2 cut(s) 68, 440
BclI TGATCA 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 3 cut(s) 242, 275, 488
BfoI RGCGCY 1 cut(s) 133
Bme18I GGWCC 3 cut(s) 11, 407, 508
BmgT120I GGNCC 3 cut(s) 11, 407, 508
BmiI GGNNCC 1 cut(s) 140
BmsI GCATC 1 cut(s) 338
BpmI CTGGAG 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 602
BsaI GGTCTC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 523
Bse3DI GCAATG 1 cut(s) 435
BseGI GGATG 2 cut(s) 290, 332
BseLI CCNNNNNNNGG 1 cut(s) 523
BseMI GCAATG 1 cut(s) 435
BshFI GGCC 2 cut(s) 54, 431
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 1 cut(s) 523
BsmAI GTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 362
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 2 cut(s) 54, 431
Bso31I GGTCTC 1 cut(s) 30
Bsp143I GATC 2 cut(s) 138, 181
BspANI GGCC 2 cut(s) 54, 431
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 140
BspPI GGATC 2 cut(s) 133, 146
BspQI GCTCTTC 1 cut(s) 570
BspTNI GGTCTC 1 cut(s) 30
BsrDI GCAATG 1 cut(s) 435
BssMI GATC 2 cut(s) 138, 181
BssNAI GTATAC 1 cut(s) 398
Bst1107I GTATAC 1 cut(s) 398
Bst4CI ACNGT 3 cut(s) 191, 353, 462
Bst6I CTCTTC 1 cut(s) 570
BstC8I GCNNGC 1 cut(s) 493
BstEII GGTNACC 1 cut(s) 586
BstF5I GGATG 2 cut(s) 290, 332
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 2 cut(s) 141, 184
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 2 cut(s) 138, 181
BstMWI GCNNNNNNNGC 2 cut(s) 275, 437
BstNSI RCATGY 1 cut(s) 249
BstPI GGTNACC 1 cut(s) 586
BstX2I RGATCY 1 cut(s) 138
BstYI RGATCY 1 cut(s) 138
BstZ17I GTATAC 1 cut(s) 398
BsuRI GGCC 2 cut(s) 54, 431
BtsCI GGATG 2 cut(s) 290, 332
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 493
CciI TCATGA 1 cut(s) 478
CfoI GCGC 1 cut(s) 132
Cfr13I GGNCC 3 cut(s) 11, 407, 508
CseI GACGC 1 cut(s) 77
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 2 cut(s) 246, 479
DpnI GATC 2 cut(s) 140, 183
DpnII GATC 2 cut(s) 138, 181
EaeI YGGCCR 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco31I GGTCTC 1 cut(s) 30
Eco47I GGWCC 3 cut(s) 11, 407, 508
Eco47III AGCGCT 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 485
Eco91I GGTNACC 1 cut(s) 586
EcoO65I GGTNACC 1 cut(s) 586
FaeI CATG 2 cut(s) 249, 482
FalI AAGNNNNNCTT 4 cut(s) 372, 404, 564, 596
FaqI GGGAC 1 cut(s) 362
FatI CATG 2 cut(s) 245, 478
FbaI TGATCA 1 cut(s) 181
FblI GTMKAC 1 cut(s) 397
FokI GGATG 2 cut(s) 277, 319
FspBI CTAG 3 cut(s) 242, 275, 488
GlaI GCGC 1 cut(s) 131
GsuI CTGGAG 1 cut(s) 146
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 2 cut(s) 54, 431
HgaI GACGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 132
Hin1II CATG 2 cut(s) 249, 482
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HindIII AAGCTT 3 cut(s) 109, 153, 338
HinfI GANTC 2 cut(s) 230, 472
HphI GGTGA 2 cut(s) 225, 598
Hpy166II GTNNAC 3 cut(s) 386, 398, 638
Hpy188I TCNGA 3 cut(s) 67, 143, 568
Hpy188III TCNNGA 5 cut(s) 159, 179, 410, 479, 517
Hpy8I GTNNAC 3 cut(s) 386, 398, 638
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 1 cut(s) 506
HpyCH4III ACNGT 3 cut(s) 191, 353, 462
HpyCH4V TGCA 4 cut(s) 150, 329, 362, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 437
Hsp92II CATG 2 cut(s) 249, 482
HspAI GCGC 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 2 cut(s) 138, 181
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 1 cut(s) 127
LweI GCATC 1 cut(s) 338
MaeI CTAG 3 cut(s) 242, 275, 488
MaeIII GTNAC 3 cut(s) 97, 170, 586
MalI GATC 2 cut(s) 140, 183
MboI GATC 2 cut(s) 138, 181
MboII GAAGA 2 cut(s) 491, 587
MflI RGATCY 1 cut(s) 138
MluCI AATT 6 cut(s) 145, 520, 561, 629, 646, 667
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 6 cut(s) 37, 97, 193, 304, 495, 577
MseI TTAA 2 cut(s) 255, 342
Mva1269I GAATGC 1 cut(s) 71
MwoI GCNNNNNNNGC 2 cut(s) 275, 437
NdeII GATC 2 cut(s) 138, 181
NlaIII CATG 2 cut(s) 249, 482
NlaIV GGNNCC 1 cut(s) 140
NmuCI GTSAC 3 cut(s) 97, 170, 586
NspI RCATGY 1 cut(s) 249
PagI TCATGA 1 cut(s) 478
PciSI GCTCTTC 1 cut(s) 570
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 2 cut(s) 230, 472
PspEI GGTNACC 1 cut(s) 586
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 3 cut(s) 11, 407, 508
PsuI RGATCY 1 cut(s) 138
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 2 cut(s) 255, 342
Sau3AI GATC 2 cut(s) 138, 181
Sau96I GGNCC 3 cut(s) 11, 407, 508
SfaNI GCATC 1 cut(s) 338
SinI GGWCC 3 cut(s) 11, 407, 508
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 6 cut(s) 145, 520, 561, 629, 646, 667
SspI AATATT 1 cut(s) 224
SspMI CTAG 3 cut(s) 242, 275, 488
TaaI ACNGT 3 cut(s) 191, 353, 462
TaqI TCGA 1 cut(s) 114
TasI AATT 6 cut(s) 145, 520, 561, 629, 646, 667
TfiI GAWTC 2 cut(s) 230, 472
Tru1I TTAA 2 cut(s) 255, 342
Tru9I TTAA 2 cut(s) 255, 342
TscAI CASTG 1 cut(s) 213
TseFI GTSAC 3 cut(s) 97, 170, 586
Tsp45I GTSAC 3 cut(s) 97, 170, 586
TspDTI ATGAA 4 cut(s) 243, 273, 464, 495
TspGWI ACGGA 2 cut(s) 85, 500
TspRI CASTG 1 cut(s) 213
VpaK11BI GGWCC 3 cut(s) 11, 407, 508
XapI RAATTY 4 cut(s) 520, 561, 629, 667
XceI RCATGY 1 cut(s) 249
XmiI GTMKAC 1 cut(s) 397
XspI CTAG 3 cut(s) 242, 275, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.