MD17G1122600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008528
Physical Location & Seq
Forward (+)
10642935 .. 10645147
2213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1122600.v1.1.491

Sequence Viewer

Length: 714 bp
ATGAAGGTTGGTTCGAAGGTAGTGTTTCTGCTGAGAGACTCAGAGGGCTTCGGCGAAGCCATCTCAGCTGCTTTCCGCCCAAATCCAAGCAATTCTACTGTAGAAGAAAGTTTCGAGCTTTCATTGGAGCGCTATGGAATCCAAAATTGCAAAGCTTCTGGAACCCTTCGCCACTTCCTCGATCCCCAGGGCCAATATGAGGTGTCTGTGCTGCTTATGGAATACCATGAACCGCCAATACTAGCCTGCGCTATTAGTGAAGTTCTAGCACAATTAGCAGGGCAGAATTCATCCTCAGTTCCCACAGTCGTTGCCCCGTTTTTCCTAGAGTCATCCAAGCTAAAGGGGGAAAGTAAATCTGCAACAAAATTTGAAAGCAAATGTTCACTTTATGGTATAGAGATTGGTTCAGAAACAGCCATAAGCAAGGCTATGGCAACCAAAACCCAGAAGCCACAGCCATCCTTGCAGATTCATCATGAACCTTTGGCTTGCTTTCTTCAGTTGGTCCGTGTTTTGAAGTTGCCAGTATATGTTCTTATTGGGCAAAGAGGTCAACACGTTTATGATAAAGAAGAGTTTCAGATACTTTATGAGATTGGAGAACTTTTAGCAAGCACTTTGAACCTTTCCTTTTCAAGAGACAAGATCACATGGAATCCAACAAGAAAATCAAAAGACGATCGGGAGCCATGGCGTGCGTTATATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

26.42

Weight (kDa)

6.54

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7894 PF25428 1 - 237 3.3e-106 Domain of unknown function (DUF7894)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 76, 233
AclWI GGATC 1 cut(s) 176
AcsI RAATTY 2 cut(s) 286, 368
AcuI CTGAAG 1 cut(s) 485
AfeI AGCGCT 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 199
AflIII ACRYGT 1 cut(s) 559
AgsI TTSAA 4 cut(s) 374, 520, 625, 639
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 4 cut(s) 68, 118, 155, 340
AluI AGCT 4 cut(s) 68, 118, 155, 340
Alw26I GTCTC 2 cut(s) 30, 636
AlwI GGATC 1 cut(s) 176
Aor51HI AGCGCT 1 cut(s) 131
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 2 cut(s) 68, 211
ApoI RAATTY 2 cut(s) 286, 368
Asp700I GAANNNNTTC 1 cut(s) 579
AspLEI GCGC 2 cut(s) 132, 251
AspS9I GGNCC 2 cut(s) 190, 508
AsuII TTCGAA 1 cut(s) 14
AvaII GGWCC 1 cut(s) 508
BbvI GCAGC 2 cut(s) 55, 198
BccI CCATC 2 cut(s) 68, 469
BciT130I CCWGG 1 cut(s) 188
BcoDI GTCTC 2 cut(s) 30, 636
BfaI CTAG 3 cut(s) 242, 266, 326
BfmI CTRYAG 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 133
BisI GCNGC 2 cut(s) 69, 212
BlsI GCNGC 2 cut(s) 70, 213
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 508
BmgT120I GGNCC 2 cut(s) 190, 508
BmiI GGNNCC 2 cut(s) 163, 690
BmrFI CCNGG 1 cut(s) 188
Bpu14I TTCGAA 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 186, 187, 692
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 527
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 3 cut(s) 186, 187, 692
BseGI GGATG 3 cut(s) 290, 332, 461
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMII CTCAG 4 cut(s) 23, 54, 78, 309
BseNI ACTGG 1 cut(s) 527
BseXI GCAGC 2 cut(s) 55, 198
Bsh1285I CGRYCG 1 cut(s) 685
BshFI GGCC 1 cut(s) 192
BsiEI CGRYCG 1 cut(s) 685
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 2 cut(s) 30, 636
BsnI GGCC 1 cut(s) 192
Bsp119I TTCGAA 1 cut(s) 14
Bsp143I GATC 3 cut(s) 181, 648, 682
Bsp19I CCATGG 1 cut(s) 692
BspACI CCGC 2 cut(s) 76, 233
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 4 cut(s) 24, 53, 77, 308
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 2 cut(s) 163, 690
BspPI GGATC 1 cut(s) 176
BspT104I TTCGAA 1 cut(s) 14
BsrI ACTGG 1 cut(s) 527
BssECI CCNNGG 3 cut(s) 186, 187, 692
BssMI GATC 3 cut(s) 181, 648, 682
BssT1I CCWWGG 1 cut(s) 692
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 2 cut(s) 100, 307
Bst6I CTCTTC 1 cut(s) 570
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 4 cut(s) 247, 493, 616, 699
BstDEI CTNAG 4 cut(s) 32, 40, 64, 295
BstDSI CCRYGG 1 cut(s) 692
BstF5I GGATG 3 cut(s) 290, 332, 461
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 2 cut(s) 132, 251
BstKTI GATC 3 cut(s) 184, 651, 685
BstMAI GTCTC 2 cut(s) 30, 636
BstMBI GATC 3 cut(s) 181, 648, 682
BstMCI CGRYCG 1 cut(s) 685
BstMWI GCNNNNNNNGC 3 cut(s) 65, 275, 466
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 186
BstSFI CTRYAG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 55, 198
BsuRI GGCC 1 cut(s) 192
BtgI CCRYGG 1 cut(s) 692
BtsCI GGATG 3 cut(s) 290, 332, 461
Cac8I GCNNGC 4 cut(s) 247, 493, 616, 699
CciI TCATGA 1 cut(s) 478
CfoI GCGC 2 cut(s) 132, 251
Cfr13I GGNCC 2 cut(s) 190, 508
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 4 cut(s) 227, 479, 654, 693
DdeI CTNAG 4 cut(s) 32, 40, 64, 295
DpnI GATC 3 cut(s) 183, 650, 684
DpnII GATC 3 cut(s) 181, 648, 682
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
EciI GGCGGA 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 692
Eco47I GGWCC 1 cut(s) 508
Eco47III AGCGCT 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 485
EcoRI GAATTC 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 692
ErhI CCWWGG 1 cut(s) 692
FaeI CATG 4 cut(s) 230, 482, 657, 696
FatI CATG 4 cut(s) 226, 478, 653, 692
Fnu4HI GCNGC 2 cut(s) 69, 212
FokI GGATG 3 cut(s) 277, 319, 448
Fsp4HI GCNGC 2 cut(s) 69, 212
FspBI CTAG 3 cut(s) 242, 266, 326
GlaI GCGC 2 cut(s) 131, 250
GluI GCNGC 2 cut(s) 69, 212
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 1 cut(s) 192
HhaI GCGC 2 cut(s) 132, 251
Hin1II CATG 4 cut(s) 230, 482, 657, 696
Hin6I GCGC 2 cut(s) 130, 249
HinP1I GCGC 2 cut(s) 130, 249
HincII GTYRAC 1 cut(s) 557
HindII GTYRAC 1 cut(s) 557
HindIII AAGCTT 1 cut(s) 153
HinfI GANTC 5 cut(s) 38, 138, 329, 472, 658
Hpy166II GTNNAC 2 cut(s) 386, 557
Hpy188I TCNGA 3 cut(s) 43, 412, 585
Hpy188III TCNNGA 4 cut(s) 159, 479, 639, 686
Hpy8I GTNNAC 2 cut(s) 386, 557
HpyAV CCTTC 2 cut(s) 10, 176
HpyCH4III ACNGT 2 cut(s) 100, 307
HpyCH4IV ACGT 1 cut(s) 561
HpyCH4V TGCA 3 cut(s) 150, 362, 469
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 275, 466
HpyF3I CTNAG 4 cut(s) 32, 40, 64, 295
HpySE526I ACGT 1 cut(s) 561
Hsp92II CATG 4 cut(s) 230, 482, 657, 696
HspAI GCGC 2 cut(s) 130, 249
Kzo9I GATC 3 cut(s) 181, 648, 682
LmnI GCTCC 2 cut(s) 127, 688
LpnPI CCDG 7 cut(s) 144, 173, 200, 259, 264, 461, 540
Lsp1109I GCAGC 2 cut(s) 55, 198
MaeI CTAG 3 cut(s) 242, 266, 326
MaeII ACGT 1 cut(s) 561
MalI GATC 3 cut(s) 183, 650, 684
MboI GATC 3 cut(s) 181, 648, 682
MboII GAAGA 3 cut(s) 116, 491, 587
MluCI AATT 5 cut(s) 91, 145, 272, 286, 368
MlyI GAGTC 2 cut(s) 32, 338
MmeI TCCRAC 1 cut(s) 686
MnlI CCTC 5 cut(s) 37, 188, 193, 304, 545
MroXI GAANNNNTTC 1 cut(s) 579
MseI TTAA 1 cut(s) 712
MslI CAYNNNNRTG 1 cut(s) 564
MspA1I CMGCKG 1 cut(s) 68
MspR9I CCNGG 1 cut(s) 188
MvaI CCWGG 1 cut(s) 188
MwoI GCNNNNNNNGC 3 cut(s) 65, 275, 466
NcoI CCATGG 1 cut(s) 692
NdeII GATC 3 cut(s) 181, 648, 682
NlaIII CATG 4 cut(s) 230, 482, 657, 696
NlaIV GGNNCC 2 cut(s) 163, 690
NspV TTCGAA 1 cut(s) 14
PagI TCATGA 1 cut(s) 478
PasI CCCWGGG 1 cut(s) 187
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 3 cut(s) 138, 472, 658
PkrI GCNGC 2 cut(s) 70, 213
Ple19I CGATCG 1 cut(s) 685
PleI GAGTC 2 cut(s) 32, 337
PpsI GAGTC 2 cut(s) 32, 337
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 2 cut(s) 163, 690
PspPI GGNCC 2 cut(s) 190, 508
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
PvuI CGATCG 1 cut(s) 685
PvuII CAGCTG 1 cut(s) 68
RseI CAYNNNNRTG 1 cut(s) 564
SaqAI TTAA 1 cut(s) 712
SatI GCNGC 2 cut(s) 69, 212
Sau3AI GATC 3 cut(s) 181, 648, 682
Sau96I GGNCC 2 cut(s) 190, 508
SchI GAGTC 2 cut(s) 32, 338
ScrFI CCNGG 1 cut(s) 188
SfcI CTRYAG 1 cut(s) 99
SfuI TTCGAA 1 cut(s) 14
SinI GGWCC 1 cut(s) 508
SmiMI CAYNNNNRTG 1 cut(s) 564
Sse9I AATT 5 cut(s) 91, 145, 272, 286, 368
SsiI CCGC 2 cut(s) 76, 233
SspMI CTAG 3 cut(s) 242, 266, 326
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 1 cut(s) 692
TaaI ACNGT 2 cut(s) 100, 307
TaiI ACGT 1 cut(s) 564
TaqI TCGA 3 cut(s) 14, 114, 180
TasI AATT 5 cut(s) 91, 145, 272, 286, 368
TfiI GAWTC 3 cut(s) 138, 472, 658
Tru1I TTAA 1 cut(s) 712
Tru9I TTAA 1 cut(s) 712
TseI GCWGC 2 cut(s) 68, 211
TspDTI ATGAA 6 cut(s) 17, 111, 243, 279, 464, 495
TspGWI ACGGA 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 508
XapI RAATTY 2 cut(s) 286, 368
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 3 cut(s) 242, 266, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.