Rh2BG523400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008528
Physical Location & Seq
Reverse (-)
73365263 .. 73367751
2489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG523400.1

Sequence Viewer

Length: 759 bp
ATGAACCGCCGCGTTTGGTTTGAAGGAAGAGCAGAGAGAAAGGAAATGGAGGTAGGTCCAAAGGTAGTGCTTCTGTTGAGAGACCCAGAGGGCTTCGGCCAAGCCATCTCCGACGCGTTCCACCCTAACCCAACTGCTTCCGTCACAGAGGAAAGCTTCGAGCTTTCGCTGGAGCGCTATGGGATCCGAAATTGCAAAGCTTCTGGAAAAGTTCGTCACTTTCTTGATCAGCACGGTCAATATGAGGTGTCAGTGTTGCTTATGGAATATTATGAATCACCGATACTAGCATGTGCTTTTAATGGAGTTCTGGCTGAACTAGCAGGACAAAAATCATCCTCAATGCCCACCATTGTTGCCCCCTTTTTCGTTGCATCATCCAAGCTTAAATGGGACAGTAAATCTGCAACAAAGTTTGACAGCAAAGGTTCACTTTATGGTATACAGATAGGTCCAGAAACAGACATAAGCAAGGCCATCATTGCCAGAACCCAGAAAGCACCACCGTCATTACAGATTCATCATGAACCTCTAGCCTGCCTTCTTCAGTTGGTCCGTGTCCTGAATTTGCCAACTCTTGTTCTTATAGGGCAAAGGGGTCAGCGAATTTCTGATAAAGAAGAGCTTGAGATACTTTATGAAATTGGGGAACTTTTGGCAAACACTTGTAAGGTAAACTTCTCAAGAGGCAAAATCACATGGAAGCCAACAAAAAAATCGAAGGATGAACAGGAGCCATGGCGTGCATTGTATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

28.28

Weight (kDa)

8.58

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7894 PF25428 16 - 252 4.1e-109 Domain of unknown function (DUF7894)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 442
AccII CGCG 2 cut(s) 12, 116
AciI CCGC 2 cut(s) 7, 10
AclWI GGATC 2 cut(s) 178, 191
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 2 cut(s) 565, 606
AcuI CTGAAG 1 cut(s) 530
AfeI AGCGCT 1 cut(s) 176
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 1 cut(s) 23
AluBI AGCT 5 cut(s) 156, 163, 200, 385, 625
AluI AGCT 5 cut(s) 156, 163, 200, 385, 625
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 2 cut(s) 178, 191
Aor51HI AGCGCT 1 cut(s) 176
AoxI GGCC 2 cut(s) 97, 474
ApoI RAATTY 2 cut(s) 565, 606
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 3 cut(s) 56, 452, 553
AsuHPI GGTGA 1 cut(s) 270
AvaII GGWCC 3 cut(s) 56, 452, 553
BamHI GGATCC 1 cut(s) 183
BccI CCATC 2 cut(s) 113, 485
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 3 cut(s) 287, 320, 533
BfoI RGCGCY 1 cut(s) 178
BisI GCNGC 1 cut(s) 10
BlsI GCNGC 1 cut(s) 11
Bme18I GGWCC 3 cut(s) 56, 452, 553
BmgT120I GGNCC 3 cut(s) 56, 452, 553
BmiI GGNNCC 2 cut(s) 185, 735
BmsI GCATC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 191
BpuEI CTTGAG 2 cut(s) 647, 667
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 737
Bse3DI GCAATG 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 737
BseGI GGATG 3 cut(s) 335, 377, 730
BseMI GCAATG 1 cut(s) 480
Bsh1236I CGCG 2 cut(s) 12, 116
BshFI GGCC 2 cut(s) 99, 476
BslFI GGGAC 1 cut(s) 407
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 1 cut(s) 407
BsnI GGCC 2 cut(s) 99, 476
Bso31I GGTCTC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 183, 226
Bsp19I CCATGG 1 cut(s) 737
BspACI CCGC 2 cut(s) 7, 10
BspANI GGCC 2 cut(s) 99, 476
BspFNI CGCG 2 cut(s) 12, 116
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 2 cut(s) 185, 735
BspPI GGATC 2 cut(s) 178, 191
BspQI GCTCTTC 2 cut(s) 22, 615
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 480
BssECI CCNNGG 1 cut(s) 737
BssMI GATC 2 cut(s) 183, 226
BssNAI GTATAC 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 737
Bst1107I GTATAC 1 cut(s) 443
Bst4CI ACNGT 3 cut(s) 236, 398, 507
Bst6I CTCTTC 2 cut(s) 22, 615
BstC8I GCNNGC 2 cut(s) 538, 744
BstDSI CCRYGG 1 cut(s) 737
BstF5I GGATG 3 cut(s) 335, 377, 730
BstFNI CGCG 2 cut(s) 12, 116
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 2 cut(s) 186, 229
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 2 cut(s) 183, 226
BstMWI GCNNNNNNNGC 2 cut(s) 320, 482
BstNSI RCATGY 1 cut(s) 294
BstUI CGCG 2 cut(s) 12, 116
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BstZ17I GTATAC 1 cut(s) 443
BsuRI GGCC 2 cut(s) 99, 476
BtgI CCRYGG 1 cut(s) 737
BtsCI GGATG 3 cut(s) 335, 377, 730
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 2 cut(s) 538, 744
CciI TCATGA 1 cut(s) 523
CfoI GCGC 1 cut(s) 177
Cfr13I GGNCC 3 cut(s) 56, 452, 553
CseI GACGC 1 cut(s) 122
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CviAII CATG 4 cut(s) 291, 524, 699, 738
DpnI GATC 2 cut(s) 185, 228
DpnII GATC 2 cut(s) 183, 226
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 2 cut(s) 22, 615
EarI CTCTTC 2 cut(s) 22, 615
Eco130I CCWWGG 1 cut(s) 737
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 3 cut(s) 56, 452, 553
Eco47III AGCGCT 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 530
EcoT14I CCWWGG 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 737
FaeI CATG 4 cut(s) 294, 527, 702, 741
FalI AAGNNNNNCTT 6 cut(s) 417, 449, 609, 641, 662, 694
FaqI GGGAC 1 cut(s) 407
FatI CATG 4 cut(s) 290, 523, 698, 737
FbaI TGATCA 1 cut(s) 226
FblI GTMKAC 1 cut(s) 442
Fnu4HI GCNGC 1 cut(s) 10
FokI GGATG 3 cut(s) 322, 364, 737
Fsp4HI GCNGC 1 cut(s) 10
FspBI CTAG 3 cut(s) 287, 320, 533
GlaI GCGC 1 cut(s) 176
GluI GCNGC 1 cut(s) 10
GsuI CTGGAG 1 cut(s) 191
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 2 cut(s) 99, 476
HgaI GACGC 1 cut(s) 122
HhaI GCGC 1 cut(s) 177
Hin1II CATG 4 cut(s) 294, 527, 702, 741
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HindIII AAGCTT 3 cut(s) 154, 198, 383
HinfI GANTC 2 cut(s) 275, 517
HphI GGTGA 1 cut(s) 270
Hpy166II GTNNAC 3 cut(s) 431, 443, 676
Hpy188I TCNGA 3 cut(s) 112, 188, 613
Hpy188III TCNNGA 6 cut(s) 204, 224, 455, 524, 562, 684
Hpy8I GTNNAC 3 cut(s) 431, 443, 676
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 3 cut(s) 17, 551, 715
HpyCH4III ACNGT 3 cut(s) 236, 398, 507
HpyCH4V TGCA 4 cut(s) 195, 374, 407, 746
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 482
Hsp92II CATG 4 cut(s) 294, 527, 702, 741
HspAI GCGC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 2 cut(s) 183, 226
LguI GCTCTTC 2 cut(s) 22, 615
LmnI GCTCC 2 cut(s) 172, 733
LweI GCATC 1 cut(s) 383
MaeI CTAG 3 cut(s) 287, 320, 533
MaeIII GTNAC 2 cut(s) 142, 215
MalI GATC 2 cut(s) 185, 228
MboI GATC 2 cut(s) 183, 226
MboII GAAGA 3 cut(s) 39, 536, 632
MflI RGATCY 1 cut(s) 183
MluCI AATT 4 cut(s) 190, 565, 606, 642
MluI ACGCGT 1 cut(s) 114
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 7 cut(s) 43, 82, 142, 238, 349, 540, 680
MseI TTAA 3 cut(s) 300, 387, 757
MvnI CGCG 2 cut(s) 12, 116
MwoI GCNNNNNNNGC 2 cut(s) 320, 482
NcoI CCATGG 1 cut(s) 737
NdeII GATC 2 cut(s) 183, 226
NlaIII CATG 4 cut(s) 294, 527, 702, 741
NlaIV GGNNCC 2 cut(s) 185, 735
NmuCI GTSAC 2 cut(s) 142, 215
NspI RCATGY 1 cut(s) 294
PagI TCATGA 1 cut(s) 523
PciSI GCTCTTC 2 cut(s) 22, 615
PfeI GAWTC 2 cut(s) 275, 517
PkrI GCNGC 1 cut(s) 11
PspN4I GGNNCC 2 cut(s) 185, 735
PspPI GGNCC 3 cut(s) 56, 452, 553
PsuI RGATCY 1 cut(s) 183
SapI GCTCTTC 2 cut(s) 22, 615
SaqAI TTAA 3 cut(s) 300, 387, 757
SatI GCNGC 1 cut(s) 10
Sau3AI GATC 2 cut(s) 183, 226
Sau96I GGNCC 3 cut(s) 56, 452, 553
SfaNI GCATC 1 cut(s) 383
SinI GGWCC 3 cut(s) 56, 452, 553
SmlI CTYRAG 2 cut(s) 626, 682
SmoI CTYRAG 2 cut(s) 626, 682
Sse9I AATT 4 cut(s) 190, 565, 606, 642
SsiI CCGC 2 cut(s) 7, 10
SspI AATATT 1 cut(s) 269
SspMI CTAG 3 cut(s) 287, 320, 533
StyI CCWWGG 1 cut(s) 737
TaaI ACNGT 3 cut(s) 236, 398, 507
TaqI TCGA 2 cut(s) 159, 719
TasI AATT 4 cut(s) 190, 565, 606, 642
TauI GCSGC 1 cut(s) 12
TfiI GAWTC 2 cut(s) 275, 517
Tru1I TTAA 3 cut(s) 300, 387, 757
Tru9I TTAA 3 cut(s) 300, 387, 757
TscAI CASTG 1 cut(s) 258
TseFI GTSAC 2 cut(s) 142, 215
Tsp45I GTSAC 2 cut(s) 142, 215
TspDTI ATGAA 6 cut(s) 17, 288, 509, 540, 654, 741
TspGWI ACGGA 2 cut(s) 130, 545
TspRI CASTG 1 cut(s) 258
VpaK11BI GGWCC 3 cut(s) 56, 452, 553
XapI RAATTY 2 cut(s) 565, 606
XceI RCATGY 1 cut(s) 294
XmiI GTMKAC 1 cut(s) 442
XspI CTAG 3 cut(s) 287, 320, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.