FvH4_6g45520

Transmembrane protein 33 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013573
Physical Location & Seq
Reverse (-)
34925395 .. 34929418
4024 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45520.t1

Sequence Viewer

Length: 1032 bp
ATGGGGGAGGAGAGAGAAGACCCGCAGAGGCTGAAGAAGATAGCGGCGGCGGCCTACGACTACGAGAACGACCCCAGATGGGCCGACTACTGGGCCAACATCCTCATCCCTCCTCACATGTCCTCCAGCCCTGACGTCATCTCCCATTTCAAGCAGAAGTTCTACAAGCGCTATATTGATCCGGAGCTTCTGGTGGATACAATGTCCGTCAGCAGTCCTTCTAAGCCAACGAGGCCTTCATCATCATCATCGTCGTCCCGGTCTACAAGTGACAGTGCAGGATCAACTCCTAGAACGTCAGGAGCATCAGCAGCATCAGGAACATCACCAGCAAGTTCCCCAACTAATCTGCGTTGGGATCGCCAAACCATTGAATTTTCTGTTAATGCTTGGGTGTTTCTTGTGGCTGTGCTTGCCATTCTCCCATTGGTGCCTAGGCACCTTTCAAATAGGGCATATCGGCTTTCTTTTATGGGCACAGCATGTTCCTCTCTTTATTCCTTGTACTCGCTTTATGGAAAACCAAGGGAGTGGAACTTACAGGGTTTGCAAGTTTACTTTCGGTCAATAGTCATGGCTAAGGATTTTATCTACTTGATATACTGCGTGACCTTTGTCACGTCCCATCTTTCTCTCAAATTCGCCCTGATTCCCATTCTATGTCGGGCTCTTGTACGTGTTGCCAAGTTCCTTAGGCGCAACTTTAGTCAATCCTCATTGTACAGGAAATATTTGGAAGAGCCTTGCGTTTGGGTCGAGTCAAATACAACTACACTCAACATACTTTCTTCACATGTTGAGATTGGAAATGGCTTCCTTTTGATTATATCTTTGCTCTCGTGGCAACGCAACCTTATACAGACATTCGTATATTGGCAGTTATTGAAGCTCATGTATCATGCTCCAGTGACTTCTAACTACCATCTAAGTGTGTGGACTAAGATAGGAAGGACTGTTCATCCATTCATCCAACGCTGGGCCCCATTCTTGGAGACTCCCGTTTCTGCAGTCCAGAGATGGTGGTTAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0121

Protein Analysis

344

Amino Acids

39.33

Weight (kDa)

9.73

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM33_Pom33 PF03661 123 - 304 9e-14 Transmembrane protein 33/Nucleoporin POM33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02420 AT3G02420
fragaria_vesca FvH4_6g45520 FvH4_6g45520
malus_domestica MD09G1082200.v1.1 MD17G1072900.v1.1
prunus_persica Prupe.3G241500_v2.0.a1
pyrus_communis pycom09g00870 pycom17g07380
rosa_chinensis RchiOBHm_Chr2g0163461
rosa_laevigata RLG00000021403
rosa_multiflora Rmu_sc0012243.1_g000002
rosa_roxburghii Rroxscaffold_2G00087300
rosa_samantha Rh2AG569900 Rh2BG582100 Rh2CG551900 Rh2DG591800
rosa_wichuraiana Rw2G047180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 430, 438
AccI GTMKAC 1 cut(s) 263
AccIII TCCGGA 1 cut(s) 181
AciI CCGC 4 cut(s) 23, 44, 47, 50
AclWI GGATC 3 cut(s) 173, 289, 366
AcsI RAATTY 2 cut(s) 374, 638
AcuI CTGAAG 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 3 cut(s) 506, 675, 722
AfeI AGCGCT 1 cut(s) 170
AfiI CCNNNNNNNGG 4 cut(s) 79, 90, 976, 988
AflIII ACRYGT 3 cut(s) 117, 676, 793
AgsI TTSAA 4 cut(s) 151, 374, 447, 886
AjiI CACGTC 1 cut(s) 621
AjuI GAANNNNNNNTTGG 2 cut(s) 220, 252
AloI GAACNNNNNNTCC 2 cut(s) 939, 971
AluBI AGCT 2 cut(s) 187, 889
AluI AGCT 2 cut(s) 187, 889
Alw26I GTCTC 1 cut(s) 986
AlwI GGATC 3 cut(s) 173, 289, 366
AlwNI CAGNNNCTG 1 cut(s) 31
Aor13HI TCCGGA 1 cut(s) 181
Aor51HI AGCGCT 1 cut(s) 170
AoxI GGCC 5 cut(s) 51, 81, 93, 233, 978
ApaI GGGCCC 1 cut(s) 982
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 374, 638
AspA2I CCTAGG 1 cut(s) 434
AspLEI GCGC 2 cut(s) 171, 699
AspS9I GGNCC 4 cut(s) 81, 93, 978, 979
AsuC2I CCSGG 1 cut(s) 259
AsuHPI GGTGA 1 cut(s) 318
AvrII CCTAGG 1 cut(s) 434
AxyI CCTNAGG 1 cut(s) 692
BaeGI GKGCMC 2 cut(s) 479, 982
BanI GGYRCC 2 cut(s) 430, 438
BanII GRGCYC 2 cut(s) 670, 982
BauI CACGAG 1 cut(s) 838
BbsI GAAGAC 1 cut(s) 24
BbvI GCAGC 1 cut(s) 323
BccI CCATC 4 cut(s) 72, 633, 930, 1011
BciVI GTATCC 1 cut(s) 190
BcnI CCSGG 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 986
BfaI CTAG 2 cut(s) 291, 435
BfmI CTRYAG 1 cut(s) 1005
BfoI RGCGCY 1 cut(s) 172
BfuI GTATCC 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 232
BisI GCNGC 4 cut(s) 45, 48, 51, 312
BlnI CCTAGG 1 cut(s) 434
BlsI GCNGC 4 cut(s) 46, 49, 52, 313
Bme1390I CCNGG 1 cut(s) 259
BmgBI CACGTC 1 cut(s) 621
BmgT120I GGNCC 4 cut(s) 81, 93, 978, 979
BmiI GGNNCC 4 cut(s) 432, 440, 980, 981
BmrFI CCNGG 1 cut(s) 259
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 2 cut(s) 314, 323
BmuI ACTGGG 1 cut(s) 100
BoxI GACNNNNGTC 1 cut(s) 614
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 2 cut(s) 109, 888
Bpu10I CCTNAGC 1 cut(s) 579
BpuMI CCSGG 1 cut(s) 259
BsaAI YACGTR 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 434, 524
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 4 cut(s) 107, 125, 137, 155
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 90, 976, 988
Bse1I ACTGG 2 cut(s) 95, 905
Bse21I CCTNAGG 1 cut(s) 692
BseAI TCCGGA 1 cut(s) 181
BseDI CCNNGG 2 cut(s) 434, 524
BseGI GGATG 4 cut(s) 99, 105, 958, 966
BseLI CCNNNNNNNGG 4 cut(s) 79, 90, 976, 988
BseNI ACTGG 2 cut(s) 95, 905
BseRI GAGGAG 2 cut(s) 23, 102
BseSI GKGCMC 2 cut(s) 479, 982
BseXI GCAGC 1 cut(s) 323
BseYI CCCAGC 1 cut(s) 975
BsgI GTGCAG 1 cut(s) 297
BshFI GGCC 5 cut(s) 53, 83, 95, 235, 980
BshNI GGYRCC 2 cut(s) 430, 438
BsiSI CCGG 2 cut(s) 182, 259
BslFI GGGAC 2 cut(s) 241, 607
BslI CCNNNNNNNGG 4 cut(s) 79, 90, 976, 988
BsmAI GTCTC 1 cut(s) 986
BsmFI GGGAC 2 cut(s) 241, 607
BsnI GGCC 5 cut(s) 53, 83, 95, 235, 980
Bsp120I GGGCCC 1 cut(s) 978
Bsp1286I GDGCHC 3 cut(s) 479, 670, 982
Bsp13I TCCGGA 1 cut(s) 181
Bsp1407I TGTACA 1 cut(s) 720
Bsp143I GATC 3 cut(s) 178, 281, 358
BspACI CCGC 4 cut(s) 23, 44, 47, 50
BspANI GGCC 5 cut(s) 53, 83, 95, 235, 980
BspEI TCCGGA 1 cut(s) 181
BspLI GGNNCC 4 cut(s) 432, 440, 980, 981
BspMAI CTGCAG 1 cut(s) 1009
BspPI GGATC 3 cut(s) 173, 289, 366
BspQI GCTCTTC 1 cut(s) 732
BspT107I GGYRCC 2 cut(s) 430, 438
BsrGI TGTACA 1 cut(s) 720
BsrI ACTGG 2 cut(s) 95, 905
BssECI CCNNGG 2 cut(s) 434, 524
BssMI GATC 3 cut(s) 178, 281, 358
BssNI GRCGYC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 838
BssT1I CCWWGG 2 cut(s) 434, 524
Bst2BI CACGAG 1 cut(s) 838
Bst4CI ACNGT 2 cut(s) 275, 955
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 720
BstBAI YACGTR 1 cut(s) 677
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 5 cut(s) 222, 579, 692, 926, 939
BstF5I GGATG 4 cut(s) 99, 105, 958, 966
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 2 cut(s) 171, 699
BstKTI GATC 3 cut(s) 181, 284, 361
BstMAI GTCTC 1 cut(s) 986
BstMBI GATC 3 cut(s) 178, 281, 358
BstMWI GCNNNNNNNGC 5 cut(s) 50, 232, 311, 413, 841
BstNSI RCATGY 3 cut(s) 121, 486, 797
BstPAI GACNNNNGTC 1 cut(s) 614
BstSCI CCNGG 1 cut(s) 257
BstSFI CTRYAG 1 cut(s) 1005
BstSLI GKGCMC 2 cut(s) 479, 982
BstV1I GCAGC 1 cut(s) 323
BstV2I GAAGAC 1 cut(s) 24
BstXI CCANNNNNNTGG 1 cut(s) 531
Bsu36I CCTNAGG 1 cut(s) 692
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 5 cut(s) 53, 83, 95, 235, 980
BtrI CACGTC 1 cut(s) 621
BtsCI GGATG 4 cut(s) 99, 105, 958, 966
BtsIMutI CAGTG 2 cut(s) 280, 912
Cac8I GCNNGC 1 cut(s) 414
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 2 cut(s) 171, 699
Cfr13I GGNCC 4 cut(s) 81, 93, 978, 979
Csp6I GTAC 3 cut(s) 505, 674, 721
CviAII CATG 6 cut(s) 118, 483, 574, 794, 892, 899
CviQI GTAC 3 cut(s) 505, 674, 721
DdeI CTNAG 5 cut(s) 222, 579, 692, 926, 939
DpnI GATC 3 cut(s) 180, 283, 360
DpnII GATC 3 cut(s) 178, 281, 358
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
Eco130I CCWWGG 2 cut(s) 434, 524
Eco147I AGGCCT 1 cut(s) 235
Eco24I GRGCYC 2 cut(s) 670, 982
Eco47III AGCGCT 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 692
EcoO109I RGGNCCY 1 cut(s) 979
EcoT14I CCWWGG 2 cut(s) 434, 524
EcoT38I GRGCYC 2 cut(s) 670, 982
ErhI CCWWGG 2 cut(s) 434, 524
FaeI CATG 6 cut(s) 121, 486, 577, 797, 895, 902
FaqI GGGAC 2 cut(s) 241, 607
FatI CATG 6 cut(s) 117, 482, 573, 793, 891, 898
FauI CCCGC 1 cut(s) 30
FblI GTMKAC 1 cut(s) 263
Fnu4HI GCNGC 4 cut(s) 45, 48, 51, 312
FokI GGATG 4 cut(s) 86, 92, 945, 953
FriOI GRGCYC 2 cut(s) 670, 982
Fsp4HI GCNGC 4 cut(s) 45, 48, 51, 312
FspBI CTAG 2 cut(s) 291, 435
GlaI GCGC 2 cut(s) 170, 698
GluI GCNGC 4 cut(s) 45, 48, 51, 312
GsaI CCCAGC 1 cut(s) 979
GsuI CTGGAG 2 cut(s) 109, 888
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 5 cut(s) 53, 83, 95, 235, 980
HapII CCGG 2 cut(s) 182, 259
HhaI GCGC 2 cut(s) 171, 699
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 6 cut(s) 121, 486, 577, 797, 895, 902
Hin6I GCGC 2 cut(s) 169, 697
HinP1I GCGC 2 cut(s) 169, 697
HinfI GANTC 3 cut(s) 649, 758, 994
HpaII CCGG 2 cut(s) 182, 259
HphI GGTGA 1 cut(s) 318
Hpy166II GTNNAC 3 cut(s) 264, 556, 936
Hpy188III TCNNGA 4 cut(s) 182, 300, 318, 1012
Hpy8I GTNNAC 3 cut(s) 264, 556, 936
Hpy99I CGWCG 1 cut(s) 256
HpyAV CCTTC 3 cut(s) 228, 246, 942
HpyCH4III ACNGT 2 cut(s) 275, 955
HpyCH4IV ACGT 4 cut(s) 135, 296, 620, 676
HpyCH4V TGCA 3 cut(s) 278, 550, 1007
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 232, 311, 413, 841
HpyF3I CTNAG 5 cut(s) 222, 579, 692, 926, 939
HpySE526I ACGT 4 cut(s) 135, 296, 620, 676
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 6 cut(s) 121, 486, 577, 797, 895, 902
HspAI GCGC 2 cut(s) 169, 697
Kpn2I TCCGGA 1 cut(s) 181
Kzo9I GATC 3 cut(s) 178, 281, 358
LguI GCTCTTC 1 cut(s) 732
LmnI GCTCC 3 cut(s) 184, 302, 907
Lsp1109I GCAGC 1 cut(s) 323
LweI GCATC 2 cut(s) 314, 323
MaeI CTAG 2 cut(s) 291, 435
MaeII ACGT 4 cut(s) 135, 296, 620, 676
MaeIII GTNAC 4 cut(s) 269, 607, 616, 907
MalI GATC 3 cut(s) 180, 283, 360
MboI GATC 3 cut(s) 178, 281, 358
MboII GAAGA 5 cut(s) 29, 46, 49, 749, 780
MhlI GDGCHC 3 cut(s) 479, 670, 982
MluCI AATT 2 cut(s) 374, 638
MlyI GAGTC 2 cut(s) 767, 988
MmeI TCCRAC 1 cut(s) 994
MnlI CCTC 8 cut(s) 21, 113, 120, 123, 133, 225, 499, 724
MroI TCCGGA 1 cut(s) 181
MseI TTAA 2 cut(s) 384, 1025
MslI CAYNNNNRTG 1 cut(s) 927
MspI CCGG 2 cut(s) 182, 259
MspR9I CCNGG 1 cut(s) 259
MwoI GCNNNNNNNGC 5 cut(s) 50, 232, 311, 413, 841
NciI CCSGG 1 cut(s) 259
NdeII GATC 3 cut(s) 178, 281, 358
NlaIII CATG 6 cut(s) 121, 486, 577, 797, 895, 902
NlaIV GGNNCC 4 cut(s) 432, 440, 980, 981
NmuCI GTSAC 4 cut(s) 269, 607, 616, 907
NspI RCATGY 3 cut(s) 121, 486, 797
PceI AGGCCT 1 cut(s) 235
PciI ACATGT 2 cut(s) 117, 793
PciSI GCTCTTC 1 cut(s) 732
PfeI GAWTC 1 cut(s) 649
PkrI GCNGC 4 cut(s) 46, 49, 52, 313
PleI GAGTC 2 cut(s) 766, 988
PpsI GAGTC 2 cut(s) 766, 988
Ppu21I YACGTR 1 cut(s) 677
PscI ACATGT 2 cut(s) 117, 793
PshAI GACNNNNGTC 1 cut(s) 614
PspFI CCCAGC 1 cut(s) 975
PspN4I GGNNCC 4 cut(s) 432, 440, 980, 981
PspOMI GGGCCC 1 cut(s) 978
PspPI GGNCC 4 cut(s) 81, 93, 978, 979
PstI CTGCAG 1 cut(s) 1009
PstNI CAGNNNCTG 1 cut(s) 31
RsaI GTAC 3 cut(s) 506, 675, 722
RsaNI GTAC 3 cut(s) 505, 674, 721
RseI CAYNNNNRTG 1 cut(s) 927
SapI GCTCTTC 1 cut(s) 732
SaqAI TTAA 2 cut(s) 384, 1025
SatI GCNGC 4 cut(s) 45, 48, 51, 312
Sau3AI GATC 3 cut(s) 178, 281, 358
Sau96I GGNCC 4 cut(s) 81, 93, 978, 979
SchI GAGTC 2 cut(s) 767, 988
ScrFI CCNGG 1 cut(s) 259
SduI GDGCHC 3 cut(s) 479, 670, 982
SfaNI GCATC 2 cut(s) 314, 323
SfcI CTRYAG 1 cut(s) 1005
SmiMI CAYNNNNRTG 1 cut(s) 927
Sse9I AATT 2 cut(s) 374, 638
SseBI AGGCCT 1 cut(s) 235
SsiI CCGC 4 cut(s) 23, 44, 47, 50
SspI AATATT 1 cut(s) 731
SspMI CTAG 2 cut(s) 291, 435
StuI AGGCCT 1 cut(s) 235
StyD4I CCNGG 1 cut(s) 257
StyI CCWWGG 2 cut(s) 434, 524
TaaI ACNGT 2 cut(s) 275, 955
TaiI ACGT 4 cut(s) 138, 299, 623, 679
TaqI TCGA 1 cut(s) 756
TaqII GACCGA 1 cut(s) 552
TasI AATT 2 cut(s) 374, 638
TatI WGTACW 2 cut(s) 504, 720
TauI GCSGC 3 cut(s) 47, 50, 53
TfiI GAWTC 1 cut(s) 649
Tru1I TTAA 2 cut(s) 384, 1025
Tru9I TTAA 2 cut(s) 384, 1025
TscAI CASTG 2 cut(s) 280, 912
TseFI GTSAC 4 cut(s) 269, 607, 616, 907
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 4 cut(s) 269, 607, 616, 907
TspDTI ATGAA 3 cut(s) 228, 947, 955
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 2 cut(s) 280, 912
XapI RAATTY 2 cut(s) 374, 638
XceI RCATGY 3 cut(s) 121, 486, 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XmaJI CCTAGG 1 cut(s) 434
XmiI GTMKAC 1 cut(s) 263
XspI CTAG 2 cut(s) 291, 435
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.