Rh2AG569900

Transmembrane protein 33 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013573
Physical Location & Seq
Reverse (-)
80036493 .. 80040074
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG569900.1

Sequence Viewer

Length: 1026 bp
ATGGGGGAGGAGAGAGACGACCCACAGAAGCTGAAGAAGATAGCGGCGGCGGCTTACGACTACGAGAACGACCCGAGATGGGCCGACTACTGGGCCAACATCCTCATCCCGCCTCACATGGCCTCTAGCCCTGACGTCATCGGCCATTTCAAGCAGAAGTTCTATAAGCGCTATATTGATCCTGAGCTTCTGGTAGATGCAATGTCCGGCAGCAGTCCTCCTCCTCCACCAAAGAGGCCATCAGCATCGTCCCGGTCTACAAATGACAGTGCAGGATCAACTCCTAGAACATCAGGAACAACAGCAGCATCAGGATCATCAGCAACAAGCGCTCCAACTTCTCTGCGTTGGGATCACCAAACCATTCAGTTTTCTGTTAATGCTTGGGTGTTTCTTGTGGCTGTGCTTGCCATTTTCCCATTGGTGCCTAGGCACCTTTCGAATAGAGCATATCGGCTTTCTTTTATGGGCACAGCGTGTTCCTCTCTTTATTCCATGTACTCGCTCTATGGGAAACCGAGGGACTGGAACTTACAGGGTTTGCAAGTTTACTTTCGGTCAATAGTCATGGCAAAAGATTTTATCTACTTGATATACTGCTTGACCTTTGTCACATCCCATCTTTCTCTCAAATTCGCCTTGATTCCCATTCTATGTCGGGCTCTCGTACATGTTGCCAAGTTCCTTAGGCGCAACTTTAGTCAATCCTCATTGTACAGGAAATACTTGGAAGAGCCTTGCGTTTGGGTCGAGTCAAACACAACTACCCTCAGCATACTCTCTTCACATGCTGAGATTGGAAATGGCTTTCTTTTGATTATATCTTTGTTCTCGTGGCAGCGCAACATTATACAGACATTCGTATATTGGCAGCTATTGAAGCTCATGTATCATGCTCCGGTGACTTCTGACTACCATCTGAGTGTGTGGACTAAGATAGGGAGGACAGTTCATCCATTCATCCACCGCTACGCCCCATTCTTGGAGACACCCGTTTCTACAATCCAGAGATGGTGGTTAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0121

Protein Analysis

341

Amino Acids

39.03

Weight (kDa)

9.58

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM33_Pom33 PF03661 120 - 299 2.1e-15 Transmembrane protein 33/Nucleoporin POM33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02420 AT3G02420
fragaria_vesca FvH4_6g45520 FvH4_6g45520
malus_domestica MD09G1082200.v1.1 MD17G1072900.v1.1
prunus_persica Prupe.3G241500_v2.0.a1
pyrus_communis pycom09g00870 pycom17g07380
rosa_chinensis RchiOBHm_Chr2g0163461
rosa_laevigata RLG00000021403
rosa_multiflora Rmu_sc0012243.1_g000002
rosa_roxburghii Rroxscaffold_2G00087300
rosa_samantha Rh2AG569900 Rh2BG582100 Rh2CG551900 Rh2DG591800
rosa_wichuraiana Rw2G047180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 424, 432
AccI GTMKAC 1 cut(s) 257
AciI CCGC 5 cut(s) 44, 47, 50, 110, 967
AclWI GGATC 4 cut(s) 173, 283, 322, 360
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 632
AcuI CTGAAG 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 135
AdeI CACNNNGTG 1 cut(s) 477
AfaI GTAC 3 cut(s) 500, 669, 716
AfeI AGCGCT 2 cut(s) 170, 331
AfiI CCNNNNNNNGG 3 cut(s) 79, 90, 982
AflIII ACRYGT 1 cut(s) 670
AgsI TTSAA 2 cut(s) 151, 880
AluBI AGCT 4 cut(s) 31, 187, 874, 883
AluI AGCT 4 cut(s) 31, 187, 874, 883
Alw26I GTCTC 2 cut(s) 9, 980
AlwI GGATC 4 cut(s) 173, 283, 322, 360
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 73
Aor51HI AGCGCT 2 cut(s) 170, 331
AoxI GGCC 5 cut(s) 81, 93, 120, 142, 236
ApeKI GCWGC 4 cut(s) 210, 305, 838, 871
ApoI RAATTY 1 cut(s) 632
AspA2I CCTAGG 1 cut(s) 428
AspLEI GCGC 4 cut(s) 171, 332, 693, 843
AspS9I GGNCC 2 cut(s) 81, 93
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 2 cut(s) 347, 913
AsuII TTCGAA 1 cut(s) 440
AvaI CYCGRG 1 cut(s) 73
AvrII CCTAGG 1 cut(s) 428
AxyI CCTNAGG 1 cut(s) 686
BaeGI GKGCMC 1 cut(s) 473
BanI GGYRCC 2 cut(s) 424, 432
BanII GRGCYC 1 cut(s) 664
BauI CACGAG 1 cut(s) 832
BbvCI CCTCAGC 1 cut(s) 770
BbvI GCAGC 4 cut(s) 222, 317, 850, 883
BccI CCATC 5 cut(s) 72, 247, 627, 924, 1005
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 9, 980
BfaI CTAG 3 cut(s) 126, 285, 429
BfoI RGCGCY 2 cut(s) 172, 333
BisI GCNGC 7 cut(s) 45, 48, 51, 211, 306, 839, 872
BlnI CCTAGG 1 cut(s) 428
BlsI GCNGC 7 cut(s) 46, 49, 52, 212, 307, 840, 873
Bme1390I CCNGG 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 2 cut(s) 81, 93
BmiI GGNNCC 2 cut(s) 426, 434
BmrFI CCNGG 1 cut(s) 253
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 3 cut(s) 187, 254, 317
BmuI ACTGGG 1 cut(s) 100
BoxI GACNNNNGTC 1 cut(s) 608
Bpu10I CCTNAGC 2 cut(s) 183, 770
Bpu14I TTCGAA 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 253
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 428, 518
BsaWI WCCGGW 1 cut(s) 898
BsaXI ACNNNNNCTCC 2 cut(s) 316, 346
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 90, 982
Bse1I ACTGG 2 cut(s) 95, 530
Bse21I CCTNAGG 1 cut(s) 686
Bse3DI GCAATG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 428, 518
BseGI GGATG 5 cut(s) 99, 105, 614, 952, 960
BseLI CCNNNNNNNGG 3 cut(s) 79, 90, 982
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 4 cut(s) 174, 783, 784, 911
BseNI ACTGG 2 cut(s) 95, 530
BseRI GAGGAG 3 cut(s) 23, 210, 213
BseSI GKGCMC 1 cut(s) 473
BseXI GCAGC 4 cut(s) 222, 317, 850, 883
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 5 cut(s) 83, 95, 122, 144, 238
BshNI GGYRCC 2 cut(s) 424, 432
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 3 cut(s) 207, 253, 899
BslFI GGGAC 2 cut(s) 235, 536
BslI CCNNNNNNNGG 3 cut(s) 79, 90, 982
BsmAI GTCTC 2 cut(s) 9, 980
BsmBI CGTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 235, 536
BsnI GGCC 5 cut(s) 83, 95, 122, 144, 238
BsoBI CYCGRG 1 cut(s) 73
Bsp119I TTCGAA 1 cut(s) 440
Bsp1286I GDGCHC 2 cut(s) 473, 664
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 4 cut(s) 178, 275, 314, 352
BspACI CCGC 5 cut(s) 44, 47, 50, 110, 967
BspANI GGCC 5 cut(s) 83, 95, 122, 144, 238
BspCNI CTCAG 4 cut(s) 175, 783, 784, 912
BspLI GGNNCC 2 cut(s) 426, 434
BspPI GGATC 4 cut(s) 173, 283, 322, 360
BspQI GCTCTTC 1 cut(s) 726
BspT104I TTCGAA 1 cut(s) 440
BspT107I GGYRCC 2 cut(s) 424, 432
BsrDI GCAATG 1 cut(s) 207
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 2 cut(s) 95, 530
BssECI CCNNGG 2 cut(s) 428, 518
BssMI GATC 4 cut(s) 178, 275, 314, 352
BssNI GRCGYC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 832
BssT1I CCWWGG 1 cut(s) 428
Bst2BI CACGAG 1 cut(s) 832
Bst4CI ACNGT 2 cut(s) 269, 949
Bst6I CTCTTC 2 cut(s) 726, 787
BstACI GRCGYC 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 714
BstBI TTCGAA 1 cut(s) 440
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 6 cut(s) 183, 686, 770, 792, 920, 933
BstF5I GGATG 5 cut(s) 99, 105, 614, 952, 960
BstH2I RGCGCY 2 cut(s) 172, 333
BstHHI GCGC 4 cut(s) 171, 332, 693, 843
BstKTI GATC 4 cut(s) 181, 278, 317, 355
BstMAI GTCTC 2 cut(s) 9, 980
BstMBI GATC 4 cut(s) 178, 275, 314, 352
BstMWI GCNNNNNNNGC 4 cut(s) 50, 329, 407, 880
BstNSI RCATGY 2 cut(s) 674, 791
BstPAI GACNNNNGTC 1 cut(s) 608
BstSCI CCNGG 1 cut(s) 251
BstSLI GKGCMC 1 cut(s) 473
BstV1I GCAGC 4 cut(s) 222, 317, 850, 883
Bsu36I CCTNAGG 1 cut(s) 686
BsuRI GGCC 5 cut(s) 83, 95, 122, 144, 238
BtsCI GGATG 5 cut(s) 99, 105, 614, 952, 960
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 408
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 4 cut(s) 171, 332, 693, 843
Cfr13I GGNCC 2 cut(s) 81, 93
Csp6I GTAC 3 cut(s) 499, 668, 715
CviAII CATG 7 cut(s) 118, 496, 568, 671, 788, 886, 893
CviQI GTAC 3 cut(s) 499, 668, 715
DdeI CTNAG 6 cut(s) 183, 686, 770, 792, 920, 933
DpnI GATC 4 cut(s) 180, 277, 316, 354
DpnII GATC 4 cut(s) 178, 275, 314, 352
DraIII CACNNNGTG 1 cut(s) 477
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 2 cut(s) 726, 787
EarI CTCTTC 2 cut(s) 726, 787
Eco130I CCWWGG 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 664
Eco47III AGCGCT 2 cut(s) 170, 331
Eco57I CTGAAG 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 686
Eco88I CYCGRG 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 428
EcoT38I GRGCYC 1 cut(s) 664
ErhI CCWWGG 1 cut(s) 428
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 7 cut(s) 121, 499, 571, 674, 791, 889, 896
FaqI GGGAC 2 cut(s) 235, 536
FatI CATG 7 cut(s) 117, 495, 567, 670, 787, 885, 892
FauI CCCGC 1 cut(s) 117
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 7 cut(s) 45, 48, 51, 211, 306, 839, 872
FokI GGATG 5 cut(s) 86, 92, 601, 939, 947
FriOI GRGCYC 1 cut(s) 664
Fsp4HI GCNGC 7 cut(s) 45, 48, 51, 211, 306, 839, 872
FspBI CTAG 3 cut(s) 126, 285, 429
GlaI GCGC 4 cut(s) 170, 331, 692, 842
GluI GCNGC 7 cut(s) 45, 48, 51, 211, 306, 839, 872
HaeII RGCGCY 2 cut(s) 172, 333
HaeIII GGCC 5 cut(s) 83, 95, 122, 144, 238
HapII CCGG 3 cut(s) 207, 253, 899
HhaI GCGC 4 cut(s) 171, 332, 693, 843
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 7 cut(s) 121, 499, 571, 674, 791, 889, 896
Hin6I GCGC 4 cut(s) 169, 330, 691, 841
HinP1I GCGC 4 cut(s) 169, 330, 691, 841
HinfI GANTC 2 cut(s) 643, 752
HpaII CCGG 3 cut(s) 207, 253, 899
HphI GGTGA 2 cut(s) 347, 913
Hpy166II GTNNAC 3 cut(s) 258, 550, 930
Hpy188I TCNGA 2 cut(s) 910, 921
Hpy188III TCNNGA 4 cut(s) 182, 294, 312, 1006
Hpy8I GTNNAC 3 cut(s) 258, 550, 930
HpyCH4III ACNGT 2 cut(s) 269, 949
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 3 cut(s) 200, 272, 544
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 329, 407, 880
HpyF3I CTNAG 6 cut(s) 183, 686, 770, 792, 920, 933
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 7 cut(s) 121, 499, 571, 674, 791, 889, 896
HspAI GCGC 4 cut(s) 169, 330, 691, 841
Kzo9I GATC 4 cut(s) 178, 275, 314, 352
LguI GCTCTTC 1 cut(s) 726
LmnI GCTCC 2 cut(s) 337, 901
Lsp1109I GCAGC 4 cut(s) 222, 317, 850, 883
LweI GCATC 3 cut(s) 187, 254, 317
MaeI CTAG 3 cut(s) 126, 285, 429
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 2 cut(s) 610, 901
MalI GATC 4 cut(s) 180, 277, 316, 354
MboI GATC 4 cut(s) 178, 275, 314, 352
MboII GAAGA 4 cut(s) 46, 49, 743, 774
MhlI GDGCHC 2 cut(s) 473, 664
MluCI AATT 1 cut(s) 632
MlyI GAGTC 1 cut(s) 761
MmeI TCCRAC 1 cut(s) 359
MseI TTAA 2 cut(s) 378, 1019
MslI CAYNNNNRTG 1 cut(s) 921
MspI CCGG 3 cut(s) 207, 253, 899
MspR9I CCNGG 1 cut(s) 253
MwoI GCNNNNNNNGC 4 cut(s) 50, 329, 407, 880
NciI CCSGG 1 cut(s) 253
NdeII GATC 4 cut(s) 178, 275, 314, 352
NlaIII CATG 7 cut(s) 121, 499, 571, 674, 791, 889, 896
NlaIV GGNNCC 2 cut(s) 426, 434
NmuCI GTSAC 2 cut(s) 610, 901
NspI RCATGY 2 cut(s) 674, 791
NspV TTCGAA 1 cut(s) 440
PciI ACATGT 1 cut(s) 670
PciSI GCTCTTC 1 cut(s) 726
PfeI GAWTC 1 cut(s) 643
PkrI GCNGC 7 cut(s) 46, 49, 52, 212, 307, 840, 873
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
PscI ACATGT 1 cut(s) 670
PshAI GACNNNNGTC 1 cut(s) 608
PspN4I GGNNCC 2 cut(s) 426, 434
PspPI GGNCC 2 cut(s) 81, 93
PstNI CAGNNNCTG 1 cut(s) 31
RsaI GTAC 3 cut(s) 500, 669, 716
RsaNI GTAC 3 cut(s) 499, 668, 715
RseI CAYNNNNRTG 1 cut(s) 921
SapI GCTCTTC 1 cut(s) 726
SaqAI TTAA 2 cut(s) 378, 1019
SatI GCNGC 7 cut(s) 45, 48, 51, 211, 306, 839, 872
Sau3AI GATC 4 cut(s) 178, 275, 314, 352
Sau96I GGNCC 2 cut(s) 81, 93
SchI GAGTC 1 cut(s) 761
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 2 cut(s) 473, 664
SetI ASST 8 cut(s) 33, 138, 189, 438, 608, 876, 885, 1025
SfaNI GCATC 3 cut(s) 187, 254, 317
SfuI TTCGAA 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 921
Sse9I AATT 1 cut(s) 632
SsiI CCGC 5 cut(s) 44, 47, 50, 110, 967
SspMI CTAG 3 cut(s) 126, 285, 429
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 428
TaaI ACNGT 2 cut(s) 269, 949
TaiI ACGT 1 cut(s) 138
TaqI TCGA 2 cut(s) 440, 750
TaqII GACCGA 1 cut(s) 546
TasI AATT 1 cut(s) 632
TatI WGTACW 2 cut(s) 498, 714
TauI GCSGC 3 cut(s) 47, 50, 53
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 2 cut(s) 378, 1019
Tru9I TTAA 2 cut(s) 378, 1019
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 2 cut(s) 610, 901
TseI GCWGC 4 cut(s) 210, 305, 838, 871
Tsp45I GTSAC 2 cut(s) 610, 901
TspDTI ATGAA 2 cut(s) 941, 949
TspRI CASTG 1 cut(s) 274
XapI RAATTY 1 cut(s) 632
XceI RCATGY 2 cut(s) 674, 791
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XmaJI CCTAGG 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 257
XspI CTAG 3 cut(s) 126, 285, 429
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.