Rh2BG582100

Transmembrane protein 33 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013573
Physical Location & Seq
Reverse (-)
79833448 .. 79836601
3154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG582100.1

Sequence Viewer

Length: 900 bp
ATGATTTTGTTACTACCGCGTTTTCTGTTTTCTGCGAATCGGGATCCTGAGCTTCTGGTAGATGCAATGTCCGGCAGCAGTCCTCCTCCTCCGCCAAAGAGGCCATCAGCATCGTCCCGGTCTACAAATGACAGTGCAGGATCGACTCCTAGAACATCAGGAACAGCAGCAGCATCAGGAGCATCAGGATCATCAGCAACAAGCGCTCCAACTTCTCTGCGTTGGGATCACCAAACCATTCAGTTTTCTGTTAATGCTTGGGTGTTTCTTGTGGCTGTGCTTGCCATTTTCCCATTGGTGCCTAGGCACCTTTCGAATAGAGCATATCGGCTTTCTTTTATGGGCACAGCGTGTTCCTCTCTTTATTCCACATACTCGCTCTATGGGAAACCGAGGGACTGGAACTTACAGGGTTTGCAAGTTTACTTTCGGTCAATAGTCATGTCTAAAGATGTTATCTACTTGATATACTGCTTGACCTTTGTCACATCCCATCTTTCTCTCAAATTCGCCTTGATTCCCATTCTATGTCGGGCTCTCGTACATGTTGCCAAGTTCCTTAGGCGCAACTTTAGTCAATCCTCATTGTACAGGAAATACTTGGAAGAGCCTTGCGTTTGGGTCGAGTCAAACACAACTACCCTCAGCATACTCTCTTCACATGCTGAGATTGGAAATGGCTTTCTTTTGATTATATCTTTGTTCTCGTGGCAGCGCAACATTATACAGACATTCGCATATTGGCAGCTATTGAAGCTCATGTATCATGCTCCGGTGACTTCTGACTACCATCTGAGTGTGTGGACCAAGATAGGGAGGACAGTTCATCCATTCATCCACCGCTACGCCCCATTCTTGGAGACACCCGTTTCTACAATCCAGAGATGGTGGTTAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0121

Protein Analysis

299

Amino Acids

33.82

Weight (kDa)

9.98

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM33_Pom33 PF03661 78 - 257 2.1e-14 Transmembrane protein 33/Nucleoporin POM33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02420 AT3G02420
fragaria_vesca FvH4_6g45520 FvH4_6g45520
malus_domestica MD09G1082200.v1.1 MD17G1072900.v1.1
prunus_persica Prupe.3G241500_v2.0.a1
pyrus_communis pycom09g00870 pycom17g07380
rosa_chinensis RchiOBHm_Chr2g0163461
rosa_laevigata RLG00000021403
rosa_multiflora Rmu_sc0012243.1_g000002
rosa_roxburghii Rroxscaffold_2G00087300
rosa_samantha Rh2AG569900 Rh2BG582100 Rh2CG551900 Rh2DG591800
rosa_wichuraiana Rw2G047180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 298, 306
AccI GTMKAC 1 cut(s) 122
AccII CGCG 1 cut(s) 19
AciI CCGC 3 cut(s) 17, 92, 841
AclWI GGATC 5 cut(s) 38, 51, 148, 196, 234
AcsI RAATTY 1 cut(s) 506
AdeI CACNNNGTG 1 cut(s) 351
AfaI GTAC 2 cut(s) 543, 590
AfeI AGCGCT 1 cut(s) 205
AfiI CCNNNNNNNGG 2 cut(s) 813, 856
AflIII ACRYGT 1 cut(s) 544
AgsI TTSAA 1 cut(s) 754
AluBI AGCT 3 cut(s) 52, 748, 757
AluI AGCT 3 cut(s) 52, 748, 757
Alw26I GTCTC 1 cut(s) 854
AlwI GGATC 5 cut(s) 38, 51, 148, 196, 234
Aor51HI AGCGCT 1 cut(s) 205
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 5 cut(s) 75, 167, 170, 712, 745
ApoI RAATTY 1 cut(s) 506
AspA2I CCTAGG 1 cut(s) 302
AspLEI GCGC 3 cut(s) 206, 567, 717
AspS9I GGNCC 1 cut(s) 804
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 221, 787
AsuII TTCGAA 1 cut(s) 314
AvaII GGWCC 1 cut(s) 804
AvrII CCTAGG 1 cut(s) 302
AxyI CCTNAGG 1 cut(s) 560
BaeGI GKGCMC 1 cut(s) 347
BamHI GGATCC 1 cut(s) 43
BanI GGYRCC 2 cut(s) 298, 306
BanII GRGCYC 1 cut(s) 538
BauI CACGAG 1 cut(s) 706
BbvCI CCTCAGC 1 cut(s) 644
BbvI GCAGC 5 cut(s) 87, 179, 182, 724, 757
BccI CCATC 4 cut(s) 112, 501, 798, 879
BcnI CCSGG 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 854
BfaI CTAG 2 cut(s) 150, 303
BfoI RGCGCY 1 cut(s) 207
BglI GCCNNNNNGGC 1 cut(s) 100
BisI GCNGC 5 cut(s) 76, 168, 171, 713, 746
BlnI CCTAGG 1 cut(s) 302
BlsI GCNGC 5 cut(s) 77, 169, 172, 714, 747
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 804
BmgT120I GGNCC 1 cut(s) 804
BmiI GGNNCC 3 cut(s) 45, 300, 308
BmrFI CCNGG 1 cut(s) 118
BmsI GCATC 4 cut(s) 52, 119, 182, 191
BoxI GACNNNNGTC 1 cut(s) 482
Bpu10I CCTNAGC 2 cut(s) 48, 644
Bpu14I TTCGAA 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 118
BsaJI CCNNGG 2 cut(s) 302, 392
BsaWI WCCGGW 1 cut(s) 772
BsaXI ACNNNNNCTCC 2 cut(s) 190, 220
Bsc4I CCNNNNNNNGG 2 cut(s) 813, 856
Bse1I ACTGG 1 cut(s) 404
Bse21I CCTNAGG 1 cut(s) 560
Bse3DI GCAATG 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 302, 392
BseGI GGATG 3 cut(s) 488, 826, 834
BseLI CCNNNNNNNGG 2 cut(s) 813, 856
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 4 cut(s) 39, 657, 658, 785
BseNI ACTGG 1 cut(s) 404
BseRI GAGGAG 2 cut(s) 75, 78
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 5 cut(s) 87, 179, 182, 724, 757
BsgI GTGCAG 1 cut(s) 156
Bsh1236I CGCG 1 cut(s) 19
BshFI GGCC 1 cut(s) 103
BshNI GGYRCC 2 cut(s) 298, 306
BsiSI CCGG 3 cut(s) 72, 118, 773
BslFI GGGAC 2 cut(s) 100, 410
BslI CCNNNNNNNGG 2 cut(s) 813, 856
BsmAI GTCTC 1 cut(s) 854
BsmFI GGGAC 2 cut(s) 100, 410
BsnI GGCC 1 cut(s) 103
Bsp119I TTCGAA 1 cut(s) 314
Bsp1286I GDGCHC 2 cut(s) 347, 538
Bsp1407I TGTACA 1 cut(s) 588
Bsp143I GATC 4 cut(s) 43, 140, 188, 226
BspACI CCGC 3 cut(s) 17, 92, 841
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 4 cut(s) 40, 657, 658, 786
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 3 cut(s) 45, 300, 308
BspPI GGATC 5 cut(s) 38, 51, 148, 196, 234
BspQI GCTCTTC 1 cut(s) 600
BspT104I TTCGAA 1 cut(s) 314
BspT107I GGYRCC 2 cut(s) 298, 306
BsrDI GCAATG 1 cut(s) 72
BsrGI TGTACA 1 cut(s) 588
BsrI ACTGG 1 cut(s) 404
BssECI CCNNGG 2 cut(s) 302, 392
BssMI GATC 4 cut(s) 43, 140, 188, 226
BssSI CACGAG 1 cut(s) 706
BssT1I CCWWGG 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 706
Bst4CI ACNGT 2 cut(s) 134, 823
Bst6I CTCTTC 2 cut(s) 600, 661
BstAUI TGTACA 1 cut(s) 588
BstBI TTCGAA 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 5 cut(s) 48, 560, 644, 666, 794
BstF5I GGATG 3 cut(s) 488, 826, 834
BstFNI CGCG 1 cut(s) 19
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 3 cut(s) 206, 567, 717
BstKTI GATC 4 cut(s) 46, 143, 191, 229
BstMAI GTCTC 1 cut(s) 854
BstMBI GATC 4 cut(s) 43, 140, 188, 226
BstMWI GCNNNNNNNGC 5 cut(s) 100, 179, 203, 281, 754
BstNSI RCATGY 2 cut(s) 548, 665
BstPAI GACNNNNGTC 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 116
BstSLI GKGCMC 1 cut(s) 347
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 5 cut(s) 87, 179, 182, 724, 757
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
Bsu36I CCTNAGG 1 cut(s) 560
BsuRI GGCC 1 cut(s) 103
BtsCI GGATG 3 cut(s) 488, 826, 834
BtsIMutI CAGTG 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 282
CfoI GCGC 3 cut(s) 206, 567, 717
Cfr13I GGNCC 1 cut(s) 804
Csp6I GTAC 2 cut(s) 542, 589
CviAII CATG 5 cut(s) 442, 545, 662, 760, 767
CviJI RGCY 9 cut(s) 52, 103, 275, 331, 536, 610, 681, 748, 757
CviKI_1 RGCY 9 cut(s) 52, 103, 275, 331, 536, 610, 681, 748, 757
CviQI GTAC 2 cut(s) 542, 589
DdeI CTNAG 5 cut(s) 48, 560, 644, 666, 794
DpnI GATC 4 cut(s) 45, 142, 190, 228
DpnII GATC 4 cut(s) 43, 140, 188, 226
DraIII CACNNNGTG 1 cut(s) 351
Eam1104I CTCTTC 2 cut(s) 600, 661
EarI CTCTTC 2 cut(s) 600, 661
EciI GGCGGA 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 538
Eco47I GGWCC 1 cut(s) 804
Eco47III AGCGCT 1 cut(s) 205
Eco81I CCTNAGG 1 cut(s) 560
EcoT14I CCWWGG 1 cut(s) 302
EcoT38I GRGCYC 1 cut(s) 538
ErhI CCWWGG 1 cut(s) 302
FaeI CATG 5 cut(s) 445, 548, 665, 763, 770
FaqI GGGAC 2 cut(s) 100, 410
FatI CATG 5 cut(s) 441, 544, 661, 759, 766
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 5 cut(s) 76, 168, 171, 713, 746
FokI GGATG 3 cut(s) 475, 813, 821
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 5 cut(s) 76, 168, 171, 713, 746
FspBI CTAG 2 cut(s) 150, 303
GlaI GCGC 3 cut(s) 205, 566, 716
GluI GCNGC 5 cut(s) 76, 168, 171, 713, 746
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 1 cut(s) 103
HapII CCGG 3 cut(s) 72, 118, 773
HhaI GCGC 3 cut(s) 206, 567, 717
Hin1II CATG 5 cut(s) 445, 548, 665, 763, 770
Hin6I GCGC 3 cut(s) 204, 565, 715
HinP1I GCGC 3 cut(s) 204, 565, 715
HinfI GANTC 4 cut(s) 37, 145, 517, 626
HpaII CCGG 3 cut(s) 72, 118, 773
HphI GGTGA 2 cut(s) 221, 787
Hpy166II GTNNAC 3 cut(s) 123, 424, 804
Hpy188I TCNGA 2 cut(s) 784, 795
Hpy188III TCNNGA 6 cut(s) 41, 47, 159, 177, 186, 880
Hpy8I GTNNAC 3 cut(s) 123, 424, 804
HpyCH4III ACNGT 2 cut(s) 134, 823
HpyCH4V TGCA 3 cut(s) 65, 137, 418
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 179, 203, 281, 754
HpyF3I CTNAG 5 cut(s) 48, 560, 644, 666, 794
Hsp92II CATG 5 cut(s) 445, 548, 665, 763, 770
HspAI GCGC 3 cut(s) 204, 565, 715
Kzo9I GATC 4 cut(s) 43, 140, 188, 226
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 3 cut(s) 179, 211, 775
Lsp1109I GCAGC 5 cut(s) 87, 179, 182, 724, 757
LweI GCATC 4 cut(s) 52, 119, 182, 191
MaeI CTAG 2 cut(s) 150, 303
MaeIII GTNAC 3 cut(s) 9, 484, 775
MalI GATC 4 cut(s) 45, 142, 190, 228
MboI GATC 4 cut(s) 43, 140, 188, 226
MboII GAAGA 2 cut(s) 617, 648
MflI RGATCY 1 cut(s) 43
MhlI GDGCHC 2 cut(s) 347, 538
MluCI AATT 1 cut(s) 506
MlyI GAGTC 2 cut(s) 139, 635
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 9 cut(s) 93, 93, 96, 99, 367, 387, 592, 653, 810
MseI TTAA 2 cut(s) 252, 893
MslI CAYNNNNRTG 1 cut(s) 795
MspI CCGG 3 cut(s) 72, 118, 773
MspR9I CCNGG 1 cut(s) 118
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 5 cut(s) 100, 179, 203, 281, 754
NciI CCSGG 1 cut(s) 118
NdeII GATC 4 cut(s) 43, 140, 188, 226
NlaIII CATG 5 cut(s) 445, 548, 665, 763, 770
NlaIV GGNNCC 3 cut(s) 45, 300, 308
NmuCI GTSAC 2 cut(s) 484, 775
NspI RCATGY 2 cut(s) 548, 665
NspV TTCGAA 1 cut(s) 314
PciI ACATGT 1 cut(s) 544
PciSI GCTCTTC 1 cut(s) 600
PfeI GAWTC 2 cut(s) 37, 517
PkrI GCNGC 5 cut(s) 77, 169, 172, 714, 747
PleI GAGTC 2 cut(s) 139, 634
PpsI GAGTC 2 cut(s) 139, 634
PscI ACATGT 1 cut(s) 544
PshAI GACNNNNGTC 1 cut(s) 482
PspN4I GGNNCC 3 cut(s) 45, 300, 308
PspPI GGNCC 1 cut(s) 804
PsuI RGATCY 1 cut(s) 43
RsaI GTAC 2 cut(s) 543, 590
RsaNI GTAC 2 cut(s) 542, 589
RseI CAYNNNNRTG 1 cut(s) 795
SapI GCTCTTC 1 cut(s) 600
SaqAI TTAA 2 cut(s) 252, 893
SatI GCNGC 5 cut(s) 76, 168, 171, 713, 746
Sau3AI GATC 4 cut(s) 43, 140, 188, 226
Sau96I GGNCC 1 cut(s) 804
SchI GAGTC 2 cut(s) 139, 635
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 2 cut(s) 347, 538
SetI ASST 6 cut(s) 54, 312, 482, 750, 759, 899
SfaNI GCATC 4 cut(s) 52, 119, 182, 191
SfuI TTCGAA 1 cut(s) 314
SinI GGWCC 1 cut(s) 804
SmiMI CAYNNNNRTG 1 cut(s) 795
Sse9I AATT 1 cut(s) 506
SsiI CCGC 3 cut(s) 17, 92, 841
SspMI CTAG 2 cut(s) 150, 303
StyD4I CCNGG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 2 cut(s) 134, 823
TaqI TCGA 3 cut(s) 143, 314, 624
TaqII GACCGA 1 cut(s) 420
TasI AATT 1 cut(s) 506
TatI WGTACW 1 cut(s) 588
TfiI GAWTC 2 cut(s) 37, 517
Tru1I TTAA 2 cut(s) 252, 893
Tru9I TTAA 2 cut(s) 252, 893
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 2 cut(s) 484, 775
TseI GCWGC 5 cut(s) 75, 167, 170, 712, 745
Tsp45I GTSAC 2 cut(s) 484, 775
TspDTI ATGAA 2 cut(s) 815, 823
TspRI CASTG 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 804
XapI RAATTY 1 cut(s) 506
XceI RCATGY 2 cut(s) 548, 665
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XmaJI CCTAGG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 122
XspI CTAG 2 cut(s) 150, 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.