MD17G1072900.v1.1

Transmembrane protein 33 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013573
Physical Location & Seq
Forward (+)
5855638 .. 5859538
3901 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1072900.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGGGGAAGAGAGAGAGGACCCACAGCAGCTGAAGAGAGTGGCGGCGGCGGCCTACGACTACGAGAACGACCCCAGATGGGCCGACTACTGGTCCAACATCCTCATCCCTACTCACATGTCCTCCCGCAGCGACGTCATCGACCATTTCAAGCGCAAGTTCTATCAACGCTACATCGATCCTGAGCTTATGGTAGACGCAATGTCATCAGGCAGTTCTTCTCAGCCAAAGAGACCATCTGTATCGTCCCGGACAACAAATGACCAAACTCAGCCCCGTAGCGCAGGTTCAACTCCCAGAACTCCAGGGACAGCAGCAGCAGCTCCTCCAACTTCTCTGCGTTGGGATCGAAAAACCATTCAGTTTTCTGTAAATGCTTGGGTGTTTCTTGTGGCTGTGCTTGCAATTTTCCCACTGGTGCCTAAAAACCTTTCAGATAGGGCGTATCGGCTTTCCTTTATGGGCACTGCATGTTCGTCTCTGTATTCCTTGTACTCACTGTATGGGAAACCAAGGGCTTGGAACTTGCAGGCTTTGCAAGTTTACTTTCAGTCAATAATCACGACTAAGGATTTTATCTACTTGATATACTGCATTACCTTTGTCACATCACATCTTTGCCTAAAATTTGCACTGATTCCCATTCTCTGTCGGGCTCTTGAACATGTTGCCAAGTTCCTTAGGCGCAATTTTAGTCAATCCTCATTGTACAGGAAGTACCTGGAAGAGCCTTGTGTTTGGGTGGAGTCTAATACAACTACCCTCAGCATACTCTCTTCACATGCTGAGGTCGGAGTTGGCTTCCTTCTGATTATATCATTGTTCTCGTGGCAGCGCAACATTGTACAGGCATTCATGTACTGGCAGCTATTGAAGCTCATGTATCATGCCCCAGCGACTGCTGACTACCATCTGAGCGTGTGGACTAAGATCGGAAGGACAGTTCATCCCCTCATCCACCGCTATGCTCGGTTCCTGGAAACTCCTCTTTCTGCTGTTCAGAGATGGTGGTTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0121

Protein Analysis

340

Amino Acids

39.19

Weight (kDa)

9.6

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM33_Pom33 PF03661 119 - 296 1.8e-14 Transmembrane protein 33/Nucleoporin POM33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02420 AT3G02420
fragaria_vesca FvH4_6g45520 FvH4_6g45520
malus_domestica MD09G1082200.v1.1 MD17G1072900.v1.1
prunus_persica Prupe.3G241500_v2.0.a1
pyrus_communis pycom09g00870 pycom17g07380
rosa_chinensis RchiOBHm_Chr2g0163461
rosa_laevigata RLG00000021403
rosa_multiflora Rmu_sc0012243.1_g000002
rosa_roxburghii Rroxscaffold_2G00087300
rosa_samantha Rh2AG569900 Rh2BG582100 Rh2CG551900 Rh2DG591800
rosa_wichuraiana Rw2G047180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
Acc36I ACCTGC 1 cut(s) 275
AccB1I GGYRCC 1 cut(s) 418
AccI GTMKAC 1 cut(s) 195
AciI CCGC 5 cut(s) 44, 47, 50, 127, 961
AclWI GGATC 2 cut(s) 173, 354
AcsI RAATTY 1 cut(s) 626
AcuI CTGAAG 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 5 cut(s) 494, 710, 719, 846, 860
AfiI CCNNNNNNNGG 2 cut(s) 79, 90
AflIII ACRYGT 2 cut(s) 117, 664
AgsI TTSAA 4 cut(s) 151, 291, 662, 874
AhdI GACNNNNNGTC 2 cut(s) 91, 202
AjnI CCWGG 3 cut(s) 304, 720, 975
AloI GAACNNNNNNTCC 2 cut(s) 927, 959
AluBI AGCT 5 cut(s) 31, 187, 323, 868, 877
AluI AGCT 5 cut(s) 31, 187, 323, 868, 877
Alw26I GTCTC 2 cut(s) 226, 483
AlwI GGATC 2 cut(s) 173, 354
AlwNI CAGNNNCTG 2 cut(s) 31, 899
AoxI GGCC 2 cut(s) 51, 81
ApeKI GCWGC 7 cut(s) 28, 129, 314, 317, 320, 832, 865
ApoI RAATTY 1 cut(s) 626
AspLEI GCGC 4 cut(s) 156, 284, 687, 837
AspS9I GGNCC 3 cut(s) 19, 81, 93
AsuC2I CCSGG 1 cut(s) 250
AvaII GGWCC 2 cut(s) 19, 93
AxyI CCTNAGG 1 cut(s) 680
BaeGI GKGCMC 1 cut(s) 467
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 1 cut(s) 658
BauI CACGAG 1 cut(s) 826
BbvCI CCTCAGC 2 cut(s) 764, 786
BbvI GCAGC 7 cut(s) 40, 141, 326, 329, 332, 844, 877
BccI CCATC 4 cut(s) 72, 244, 918, 999
BciT130I CCWGG 3 cut(s) 306, 722, 977
BcnI CCSGG 1 cut(s) 250
BcoDI GTCTC 2 cut(s) 226, 483
BfuAI ACCTGC 1 cut(s) 275
Bme1390I CCNGG 4 cut(s) 250, 306, 722, 977
Bme18I GGWCC 2 cut(s) 19, 93
BmeRI GACNNNNNGTC 2 cut(s) 91, 202
BmgT120I GGNCC 3 cut(s) 19, 81, 93
BmiI GGNNCC 3 cut(s) 21, 420, 974
BmrFI CCNGG 4 cut(s) 250, 306, 722, 977
BpmI CTGGAG 1 cut(s) 288
Bpu10I CCTNAGC 3 cut(s) 183, 764, 786
BpuMI CCSGG 1 cut(s) 250
Bsa29I ATCGAT 1 cut(s) 177
BsaHI GRCGYC 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 226
BsaJI CCNNGG 2 cut(s) 305, 512
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 90
Bse1I ACTGG 3 cut(s) 95, 420, 866
Bse21I CCTNAGG 1 cut(s) 680
Bse3DI GCAATG 1 cut(s) 207
BseBI CCWGG 3 cut(s) 306, 722, 977
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 2 cut(s) 305, 512
BseGI GGATG 4 cut(s) 99, 105, 946, 954
BseLI CCNNNNNNNGG 2 cut(s) 79, 90
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 6 cut(s) 174, 236, 284, 777, 778, 905
BseNI ACTGG 3 cut(s) 95, 420, 866
BseRI GAGGAG 2 cut(s) 315, 975
BseSI GKGCMC 1 cut(s) 467
BseXI GCAGC 7 cut(s) 40, 141, 326, 329, 332, 844, 877
BseYI CCCAGC 1 cut(s) 892
BshFI GGCC 2 cut(s) 53, 83
BshNI GGYRCC 1 cut(s) 418
BshVI ATCGAT 1 cut(s) 177
BsiSI CCGG 1 cut(s) 250
BslFI GGGAC 2 cut(s) 232, 322
BslI CCNNNNNNNGG 2 cut(s) 79, 90
BsmAI GTCTC 2 cut(s) 226, 483
BsmBI CGTCTC 1 cut(s) 483
BsmFI GGGAC 2 cut(s) 232, 322
BsmI GAATGC 1 cut(s) 851
BsnI GGCC 2 cut(s) 53, 83
Bso31I GGTCTC 1 cut(s) 226
Bsp1286I GDGCHC 2 cut(s) 467, 658
Bsp1407I TGTACA 2 cut(s) 708, 844
Bsp143I GATC 3 cut(s) 178, 346, 930
BspACI CCGC 5 cut(s) 44, 47, 50, 127, 961
BspANI GGCC 2 cut(s) 53, 83
BspCNI CTCAG 6 cut(s) 175, 235, 283, 777, 778, 906
BspDI ATCGAT 1 cut(s) 177
BspLI GGNNCC 3 cut(s) 21, 420, 974
BspMI ACCTGC 1 cut(s) 275
BspPI GGATC 2 cut(s) 173, 354
BspQI GCTCTTC 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 418
BspTNI GGTCTC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 207
BsrGI TGTACA 2 cut(s) 708, 844
BsrI ACTGG 3 cut(s) 95, 420, 866
BssECI CCNNGG 2 cut(s) 305, 512
BssMI GATC 3 cut(s) 178, 346, 930
BssNI GRCGYC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 826
BssT1I CCWWGG 1 cut(s) 512
Bst2BI CACGAG 1 cut(s) 826
Bst2UI CCWGG 3 cut(s) 306, 722, 977
Bst4CI ACNGT 2 cut(s) 501, 943
Bst6I CTCTTC 4 cut(s) 3, 29, 720, 781
BstACI GRCGYC 1 cut(s) 135
BstAPI GCANNNNNTGC 1 cut(s) 535
BstAUI TGTACA 2 cut(s) 708, 844
BstC8I GCNNGC 2 cut(s) 402, 531
BstDEI CTNAG 9 cut(s) 183, 222, 270, 567, 680, 764, 786, 914, 927
BstF5I GGATG 4 cut(s) 99, 105, 946, 954
BstHHI GCGC 4 cut(s) 156, 284, 687, 837
BstKTI GATC 3 cut(s) 181, 349, 933
BstMAI GTCTC 2 cut(s) 226, 483
BstMBI GATC 3 cut(s) 178, 346, 930
BstMWI GCNNNNNNNGC 5 cut(s) 50, 320, 401, 535, 874
BstNI CCWGG 3 cut(s) 306, 722, 977
BstNSI RCATGY 4 cut(s) 121, 474, 668, 785
BstSCI CCNGG 4 cut(s) 248, 304, 720, 975
BstSLI GKGCMC 1 cut(s) 467
BstV1I GCAGC 7 cut(s) 40, 141, 326, 329, 332, 844, 877
BstXI CCANNNNNNTGG 1 cut(s) 519
Bsu15I ATCGAT 1 cut(s) 177
Bsu36I CCTNAGG 1 cut(s) 680
BsuRI GGCC 2 cut(s) 53, 83
BsuTUI ATCGAT 1 cut(s) 177
BtsCI GGATG 4 cut(s) 99, 105, 946, 954
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 4 cut(s) 413, 465, 497, 632
BveI ACCTGC 1 cut(s) 275
Cac8I GCNNGC 2 cut(s) 402, 531
CaiI CAGNNNCTG 2 cut(s) 31, 899
CfoI GCGC 4 cut(s) 156, 284, 687, 837
Cfr13I GGNCC 3 cut(s) 19, 81, 93
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 1 cut(s) 206
Csp6I GTAC 5 cut(s) 493, 709, 718, 845, 859
CviAII CATG 7 cut(s) 118, 471, 665, 782, 856, 880, 887
CviQI GTAC 5 cut(s) 493, 709, 718, 845, 859
DdeI CTNAG 9 cut(s) 183, 222, 270, 567, 680, 764, 786, 914, 927
DpnI GATC 3 cut(s) 180, 348, 932
DpnII GATC 3 cut(s) 178, 346, 930
DriI GACNNNNNGTC 2 cut(s) 91, 202
Eam1104I CTCTTC 4 cut(s) 3, 29, 720, 781
Eam1105I GACNNNNNGTC 2 cut(s) 91, 202
EarI CTCTTC 4 cut(s) 3, 29, 720, 781
Eco130I CCWWGG 1 cut(s) 512
Eco24I GRGCYC 1 cut(s) 658
Eco31I GGTCTC 1 cut(s) 226
Eco47I GGWCC 2 cut(s) 19, 93
Eco57I CTGAAG 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 680
EcoO109I RGGNCCY 1 cut(s) 19
EcoRII CCWGG 3 cut(s) 304, 720, 975
EcoT14I CCWWGG 1 cut(s) 512
EcoT38I GRGCYC 1 cut(s) 658
ErhI CCWWGG 1 cut(s) 512
Esp3I CGTCTC 1 cut(s) 483
FaeI CATG 7 cut(s) 121, 474, 668, 785, 859, 883, 890
FaqI GGGAC 2 cut(s) 232, 322
FatI CATG 7 cut(s) 117, 470, 664, 781, 855, 879, 886
FauI CCCGC 1 cut(s) 134
FblI GTMKAC 1 cut(s) 195
FokI GGATG 4 cut(s) 86, 92, 933, 941
FriOI GRGCYC 1 cut(s) 658
GlaI GCGC 4 cut(s) 155, 283, 686, 836
GsaI CCCAGC 1 cut(s) 896
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 2 cut(s) 53, 83
HapII CCGG 1 cut(s) 250
HgaI GACGC 1 cut(s) 206
HhaI GCGC 4 cut(s) 156, 284, 687, 837
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 7 cut(s) 121, 474, 668, 785, 859, 883, 890
Hin6I GCGC 4 cut(s) 154, 282, 685, 835
HinP1I GCGC 4 cut(s) 154, 282, 685, 835
HinfI GANTC 2 cut(s) 637, 746
HpaII CCGG 1 cut(s) 250
Hpy166II GTNNAC 3 cut(s) 196, 544, 924
Hpy188I TCNGA 6 cut(s) 436, 794, 810, 915, 935, 1002
Hpy188III TCNNGA 3 cut(s) 182, 562, 659
Hpy8I GTNNAC 3 cut(s) 196, 544, 924
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 2 cut(s) 815, 930
HpyCH4III ACNGT 2 cut(s) 501, 943
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 6 cut(s) 404, 470, 529, 538, 594, 632
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 320, 401, 535, 874
HpyF3I CTNAG 9 cut(s) 183, 222, 270, 567, 680, 764, 786, 914, 927
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 7 cut(s) 121, 474, 668, 785, 859, 883, 890
HspAI GCGC 4 cut(s) 154, 282, 685, 835
Kzo9I GATC 3 cut(s) 178, 346, 930
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 1 cut(s) 328
Lsp1109I GCAGC 7 cut(s) 40, 141, 326, 329, 332, 844, 877
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 604
MalI GATC 3 cut(s) 180, 348, 932
MboI GATC 3 cut(s) 178, 346, 930
MboII GAAGA 5 cut(s) 20, 46, 210, 737, 768
MhlI GDGCHC 2 cut(s) 467, 658
MluCI AATT 3 cut(s) 405, 626, 688
MlyI GAGTC 1 cut(s) 755
MmeI TCCRAC 3 cut(s) 120, 353, 772
MslI CAYNNNNRTG 1 cut(s) 963
MspA1I CMGCKG 1 cut(s) 31
MspI CCGG 1 cut(s) 250
MspR9I CCNGG 4 cut(s) 250, 306, 722, 977
Mva1269I GAATGC 1 cut(s) 851
MvaI CCWGG 3 cut(s) 306, 722, 977
MwoI GCNNNNNNNGC 5 cut(s) 50, 320, 401, 535, 874
NciI CCSGG 1 cut(s) 250
NdeII GATC 3 cut(s) 178, 346, 930
NlaIII CATG 7 cut(s) 121, 474, 668, 785, 859, 883, 890
NlaIV GGNNCC 3 cut(s) 21, 420, 974
NmuCI GTSAC 1 cut(s) 604
NspI RCATGY 4 cut(s) 121, 474, 668, 785
PciI ACATGT 2 cut(s) 117, 664
PciSI GCTCTTC 1 cut(s) 720
PctI GAATGC 1 cut(s) 851
PfeI GAWTC 1 cut(s) 637
PfoI TCCNGGA 2 cut(s) 248, 975
PleI GAGTC 1 cut(s) 754
PpsI GAGTC 1 cut(s) 754
PpuMI RGGWCCY 1 cut(s) 19
PscI ACATGT 2 cut(s) 117, 664
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 3 cut(s) 304, 720, 975
PspFI CCCAGC 1 cut(s) 892
PspGI CCWGG 3 cut(s) 304, 720, 975
PspN4I GGNNCC 3 cut(s) 21, 420, 974
PspPI GGNCC 3 cut(s) 19, 81, 93
PspPPI RGGWCCY 1 cut(s) 19
PsrI GAACNNNNNNTAC 2 cut(s) 271, 303
PstNI CAGNNNCTG 2 cut(s) 31, 899
PvuII CAGCTG 1 cut(s) 31
RsaI GTAC 5 cut(s) 494, 710, 719, 846, 860
RsaNI GTAC 5 cut(s) 493, 709, 718, 845, 859
RseI CAYNNNNRTG 1 cut(s) 963
SapI GCTCTTC 1 cut(s) 720
Sau3AI GATC 3 cut(s) 178, 346, 930
Sau96I GGNCC 3 cut(s) 19, 81, 93
SchI GAGTC 1 cut(s) 755
ScrFI CCNGG 4 cut(s) 250, 306, 722, 977
SduI GDGCHC 2 cut(s) 467, 658
SinI GGWCC 2 cut(s) 19, 93
SmiMI CAYNNNNRTG 1 cut(s) 963
Sse9I AATT 3 cut(s) 405, 626, 688
SsiI CCGC 5 cut(s) 44, 47, 50, 127, 961
StyD4I CCNGG 4 cut(s) 248, 304, 720, 975
StyI CCWWGG 1 cut(s) 512
TaaI ACNGT 2 cut(s) 501, 943
TaiI ACGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 141, 177, 349
TasI AATT 3 cut(s) 405, 626, 688
TatI WGTACW 4 cut(s) 492, 708, 844, 858
TauI GCSGC 3 cut(s) 47, 50, 53
TfiI GAWTC 1 cut(s) 637
TscAI CASTG 4 cut(s) 420, 472, 504, 639
TseFI GTSAC 1 cut(s) 604
TseI GCWGC 7 cut(s) 28, 129, 314, 317, 320, 832, 865
Tsp45I GTSAC 1 cut(s) 604
TspDTI ATGAA 2 cut(s) 844, 935
TspRI CASTG 4 cut(s) 420, 472, 504, 639
VpaK11BI GGWCC 2 cut(s) 19, 93
XapI RAATTY 1 cut(s) 626
XceI RCATGY 4 cut(s) 121, 474, 668, 785
XmiI GTMKAC 1 cut(s) 195
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.