Rw2G047180

Transmembrane protein 33 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013573
Physical Location & Seq
Reverse (-)
74559892 .. 74564554
4663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G047180.1

Sequence Viewer

Length: 1044 bp
ATGGGGGAGGAGAGAGACGACCCACAGAAGCTGAAGAAGATAGCGGCGGCGGCTTACGACTACGAGAACGACCCGAGATGGGCCGACTACTGGGCCAACATCCTCATCCCGCCTCACATGGCCTCTAGCCCTGACGTCATCGGCCATTTCAAGCAGAAGTTCTATAAGCGCTATATTGATCCTGAGCTTCTGGTAGATGCAATGTCCGGCAGCAGTCCTCCTCCTCCGCCAAAGAGGCCATCAGCATCGTCCCGGTCTACAAATGACAGTGCAGGATCGACTCCTAGAACATCAGGAACAGCTGCAGCATCAGGAGCATCAGGAACATCAGGATCATCAGCAACAAGCGCTCCAACTTCTCTGCGTTGGGATCACCAAACCATTCAGTTTTCTGTTAATGCTTGGGTGTTTCTTGTGGCTGTGCTTGCCATTTTCCCATTGGTGCCTAGGCACCTTTCGAATAGAGCATATCGGCTTTCTTTTATGGGCACAGCGTGTTCCTCTCTTTATTCCACGTACTCGCTCTATGGGAAACCGAGGGACTGGAACTTACAGGGTTTGCAAGTTTACTTTCGGTCAATAGTCATGTCTAAAGATGTTATCTACTTGATATACTGCTTGACCTTTGTCACATCCCATCTTTCTCTCAAATTCGCCTTGATTCCCATTCTATGTCGGGCTCTCGTACATGTTGCCAAGTTCCTTAGGCGCAACTTTAGTCAATCCTCATTGTACAGGAAATACTTGGAAGAGCCTTGTGTTTGGGTGGAGTCAAACACAACTACCCTCAGCATACTCTCTTCACATGCTGAGATTGGAAATGGCTTTCTTTTGATTATATCTTTGTTCTCGTGGCAGCGCAACATTATACAGACATTCGCATATTGGCAGCTATTGAAGCTCATGTATCATGCTCCGGTGACTTCTGACTACCATCTGAGTGTGTGGACTAAGATAGGGAGGACAGTTCATCCATTCATCCACCGCTACGCCCCATTCTTGGAGACACCCGTTTCTACAATCCAGAGATGGTGGTTAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0121

Protein Analysis

347

Amino Acids

39.37

Weight (kDa)

9.58

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM33_Pom33 PF03661 126 - 305 3.4e-14 Transmembrane protein 33/Nucleoporin POM33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02420 AT3G02420
fragaria_vesca FvH4_6g45520 FvH4_6g45520
malus_domestica MD09G1082200.v1.1 MD17G1072900.v1.1
prunus_persica Prupe.3G241500_v2.0.a1
pyrus_communis pycom09g00870 pycom17g07380
rosa_chinensis RchiOBHm_Chr2g0163461
rosa_laevigata RLG00000021403
rosa_multiflora Rmu_sc0012243.1_g000002
rosa_roxburghii Rroxscaffold_2G00087300
rosa_samantha Rh2AG569900 Rh2BG582100 Rh2CG551900 Rh2DG591800
rosa_wichuraiana Rw2G047180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 442, 450
AccI GTMKAC 1 cut(s) 257
AciI CCGC 6 cut(s) 44, 47, 50, 110, 227, 985
AclWI GGATC 4 cut(s) 173, 283, 340, 378
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 650
AcuI CTGAAG 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 135
AdeI CACNNNGTG 1 cut(s) 495
AfaI GTAC 3 cut(s) 518, 687, 734
AfeI AGCGCT 2 cut(s) 170, 349
AfiI CCNNNNNNNGG 3 cut(s) 79, 90, 1000
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 2 cut(s) 151, 898
AluBI AGCT 5 cut(s) 31, 187, 302, 892, 901
AluI AGCT 5 cut(s) 31, 187, 302, 892, 901
Alw26I GTCTC 2 cut(s) 9, 998
AlwI GGATC 4 cut(s) 173, 283, 340, 378
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 73
Aor51HI AGCGCT 2 cut(s) 170, 349
AoxI GGCC 5 cut(s) 81, 93, 120, 142, 236
ApeKI GCWGC 5 cut(s) 210, 302, 305, 856, 889
ApoI RAATTY 1 cut(s) 650
AspA2I CCTAGG 1 cut(s) 446
AspLEI GCGC 4 cut(s) 171, 350, 711, 861
AspS9I GGNCC 2 cut(s) 81, 93
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 2 cut(s) 365, 931
AsuII TTCGAA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 73
AvrII CCTAGG 1 cut(s) 446
AxyI CCTNAGG 1 cut(s) 704
BaeGI GKGCMC 1 cut(s) 491
BanI GGYRCC 2 cut(s) 442, 450
BanII GRGCYC 1 cut(s) 682
BauI CACGAG 1 cut(s) 850
BbvCI CCTCAGC 1 cut(s) 788
BbvI GCAGC 5 cut(s) 222, 289, 317, 868, 901
BccI CCATC 5 cut(s) 72, 247, 645, 942, 1023
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 9, 998
BfaI CTAG 3 cut(s) 126, 285, 447
BfmI CTRYAG 1 cut(s) 303
BfoI RGCGCY 2 cut(s) 172, 351
BglI GCCNNNNNGGC 1 cut(s) 235
BisI GCNGC 8 cut(s) 45, 48, 51, 211, 303, 306, 857, 890
BlnI CCTAGG 1 cut(s) 446
BlsI GCNGC 8 cut(s) 46, 49, 52, 212, 304, 307, 858, 891
Bme1390I CCNGG 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 2 cut(s) 81, 93
BmiI GGNNCC 2 cut(s) 444, 452
BmrFI CCNGG 1 cut(s) 253
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 4 cut(s) 187, 254, 317, 326
BmuI ACTGGG 1 cut(s) 100
BoxI GACNNNNGTC 1 cut(s) 626
Bpu10I CCTNAGC 2 cut(s) 183, 788
Bpu14I TTCGAA 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 253
BsaAI YACGTR 1 cut(s) 516
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 446, 536
BsaWI WCCGGW 1 cut(s) 916
BsaXI ACNNNNNCTCC 2 cut(s) 334, 364
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 90, 1000
Bse1I ACTGG 2 cut(s) 95, 548
Bse21I CCTNAGG 1 cut(s) 704
Bse3DI GCAATG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 446, 536
BseGI GGATG 5 cut(s) 99, 105, 632, 970, 978
BseLI CCNNNNNNNGG 3 cut(s) 79, 90, 1000
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 4 cut(s) 174, 801, 802, 929
BseNI ACTGG 2 cut(s) 95, 548
BseRI GAGGAG 3 cut(s) 23, 210, 213
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 5 cut(s) 222, 289, 317, 868, 901
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 5 cut(s) 83, 95, 122, 144, 238
BshNI GGYRCC 2 cut(s) 442, 450
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 3 cut(s) 207, 253, 917
BslFI GGGAC 2 cut(s) 235, 554
BslI CCNNNNNNNGG 3 cut(s) 79, 90, 1000
BsmAI GTCTC 2 cut(s) 9, 998
BsmBI CGTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 235, 554
BsnI GGCC 5 cut(s) 83, 95, 122, 144, 238
BsoBI CYCGRG 1 cut(s) 73
Bsp119I TTCGAA 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 491, 682
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 4 cut(s) 178, 275, 332, 370
BspACI CCGC 6 cut(s) 44, 47, 50, 110, 227, 985
BspANI GGCC 5 cut(s) 83, 95, 122, 144, 238
BspCNI CTCAG 4 cut(s) 175, 801, 802, 930
BspLI GGNNCC 2 cut(s) 444, 452
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 4 cut(s) 173, 283, 340, 378
BspQI GCTCTTC 1 cut(s) 744
BspT104I TTCGAA 1 cut(s) 458
BspT107I GGYRCC 2 cut(s) 442, 450
BsrDI GCAATG 1 cut(s) 207
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 2 cut(s) 95, 548
BssECI CCNNGG 2 cut(s) 446, 536
BssMI GATC 4 cut(s) 178, 275, 332, 370
BssNI GRCGYC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 850
BssT1I CCWWGG 1 cut(s) 446
Bst2BI CACGAG 1 cut(s) 850
Bst4CI ACNGT 2 cut(s) 269, 967
Bst6I CTCTTC 2 cut(s) 744, 805
BstACI GRCGYC 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 732
BstBAI YACGTR 1 cut(s) 516
BstBI TTCGAA 1 cut(s) 458
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 6 cut(s) 183, 704, 788, 810, 938, 951
BstF5I GGATG 5 cut(s) 99, 105, 632, 970, 978
BstH2I RGCGCY 2 cut(s) 172, 351
BstHHI GCGC 4 cut(s) 171, 350, 711, 861
BstKTI GATC 4 cut(s) 181, 278, 335, 373
BstMAI GTCTC 2 cut(s) 9, 998
BstMBI GATC 4 cut(s) 178, 275, 332, 370
BstMWI GCNNNNNNNGC 6 cut(s) 50, 235, 314, 347, 425, 898
BstNSI RCATGY 2 cut(s) 692, 809
BstPAI GACNNNNGTC 1 cut(s) 626
BstSCI CCNGG 1 cut(s) 251
BstSFI CTRYAG 1 cut(s) 303
BstSLI GKGCMC 1 cut(s) 491
BstV1I GCAGC 5 cut(s) 222, 289, 317, 868, 901
Bsu36I CCTNAGG 1 cut(s) 704
BsuRI GGCC 5 cut(s) 83, 95, 122, 144, 238
BtsCI GGATG 5 cut(s) 99, 105, 632, 970, 978
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 4 cut(s) 171, 350, 711, 861
Cfr13I GGNCC 2 cut(s) 81, 93
Csp6I GTAC 3 cut(s) 517, 686, 733
CviAII CATG 6 cut(s) 118, 586, 689, 806, 904, 911
CviQI GTAC 3 cut(s) 517, 686, 733
DdeI CTNAG 6 cut(s) 183, 704, 788, 810, 938, 951
DpnI GATC 4 cut(s) 180, 277, 334, 372
DpnII GATC 4 cut(s) 178, 275, 332, 370
DraIII CACNNNGTG 1 cut(s) 495
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 2 cut(s) 744, 805
EarI CTCTTC 2 cut(s) 744, 805
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 682
Eco47III AGCGCT 2 cut(s) 170, 349
Eco57I CTGAAG 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 704
Eco88I CYCGRG 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 446
EcoT38I GRGCYC 1 cut(s) 682
ErhI CCWWGG 1 cut(s) 446
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 6 cut(s) 121, 589, 692, 809, 907, 914
FaqI GGGAC 2 cut(s) 235, 554
FatI CATG 6 cut(s) 117, 585, 688, 805, 903, 910
FauI CCCGC 1 cut(s) 117
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 8 cut(s) 45, 48, 51, 211, 303, 306, 857, 890
FokI GGATG 5 cut(s) 86, 92, 619, 957, 965
FriOI GRGCYC 1 cut(s) 682
Fsp4HI GCNGC 8 cut(s) 45, 48, 51, 211, 303, 306, 857, 890
FspBI CTAG 3 cut(s) 126, 285, 447
GlaI GCGC 4 cut(s) 170, 349, 710, 860
GluI GCNGC 8 cut(s) 45, 48, 51, 211, 303, 306, 857, 890
HaeII RGCGCY 2 cut(s) 172, 351
HaeIII GGCC 5 cut(s) 83, 95, 122, 144, 238
HapII CCGG 3 cut(s) 207, 253, 917
HhaI GCGC 4 cut(s) 171, 350, 711, 861
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 6 cut(s) 121, 589, 692, 809, 907, 914
Hin6I GCGC 4 cut(s) 169, 348, 709, 859
HinP1I GCGC 4 cut(s) 169, 348, 709, 859
HinfI GANTC 3 cut(s) 280, 661, 770
HpaII CCGG 3 cut(s) 207, 253, 917
HphI GGTGA 2 cut(s) 365, 931
Hpy166II GTNNAC 3 cut(s) 258, 568, 948
Hpy188I TCNGA 2 cut(s) 928, 939
Hpy188III TCNNGA 6 cut(s) 182, 294, 312, 321, 330, 1024
Hpy8I GTNNAC 3 cut(s) 258, 568, 948
HpyCH4III ACNGT 2 cut(s) 269, 967
HpyCH4IV ACGT 2 cut(s) 135, 515
HpyCH4V TGCA 4 cut(s) 200, 272, 305, 562
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 235, 314, 347, 425, 898
HpyF3I CTNAG 6 cut(s) 183, 704, 788, 810, 938, 951
HpySE526I ACGT 2 cut(s) 135, 515
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 6 cut(s) 121, 589, 692, 809, 907, 914
HspAI GCGC 4 cut(s) 169, 348, 709, 859
Kzo9I GATC 4 cut(s) 178, 275, 332, 370
LguI GCTCTTC 1 cut(s) 744
LmnI GCTCC 3 cut(s) 314, 355, 919
Lsp1109I GCAGC 5 cut(s) 222, 289, 317, 868, 901
LweI GCATC 4 cut(s) 187, 254, 317, 326
MaeI CTAG 3 cut(s) 126, 285, 447
MaeII ACGT 2 cut(s) 135, 515
MaeIII GTNAC 2 cut(s) 628, 919
MalI GATC 4 cut(s) 180, 277, 334, 372
MboI GATC 4 cut(s) 178, 275, 332, 370
MboII GAAGA 4 cut(s) 46, 49, 761, 792
MhlI GDGCHC 2 cut(s) 491, 682
MluCI AATT 1 cut(s) 650
MlyI GAGTC 2 cut(s) 274, 779
MmeI TCCRAC 1 cut(s) 377
MseI TTAA 2 cut(s) 396, 1037
MslI CAYNNNNRTG 1 cut(s) 939
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 3 cut(s) 207, 253, 917
MspR9I CCNGG 1 cut(s) 253
MwoI GCNNNNNNNGC 6 cut(s) 50, 235, 314, 347, 425, 898
NciI CCSGG 1 cut(s) 253
NdeII GATC 4 cut(s) 178, 275, 332, 370
NlaIII CATG 6 cut(s) 121, 589, 692, 809, 907, 914
NlaIV GGNNCC 2 cut(s) 444, 452
NmuCI GTSAC 2 cut(s) 628, 919
NspI RCATGY 2 cut(s) 692, 809
NspV TTCGAA 1 cut(s) 458
PciI ACATGT 1 cut(s) 688
PciSI GCTCTTC 1 cut(s) 744
PfeI GAWTC 1 cut(s) 661
PkrI GCNGC 8 cut(s) 46, 49, 52, 212, 304, 307, 858, 891
PleI GAGTC 2 cut(s) 274, 778
PpsI GAGTC 2 cut(s) 274, 778
Ppu21I YACGTR 1 cut(s) 516
PscI ACATGT 1 cut(s) 688
PshAI GACNNNNGTC 1 cut(s) 626
PspN4I GGNNCC 2 cut(s) 444, 452
PspPI GGNCC 2 cut(s) 81, 93
PstI CTGCAG 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 3 cut(s) 518, 687, 734
RsaNI GTAC 3 cut(s) 517, 686, 733
RseI CAYNNNNRTG 1 cut(s) 939
SapI GCTCTTC 1 cut(s) 744
SaqAI TTAA 2 cut(s) 396, 1037
SatI GCNGC 8 cut(s) 45, 48, 51, 211, 303, 306, 857, 890
Sau3AI GATC 4 cut(s) 178, 275, 332, 370
Sau96I GGNCC 2 cut(s) 81, 93
SchI GAGTC 2 cut(s) 274, 779
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 2 cut(s) 491, 682
SfaNI GCATC 4 cut(s) 187, 254, 317, 326
SfcI CTRYAG 1 cut(s) 303
SfuI TTCGAA 1 cut(s) 458
SmiMI CAYNNNNRTG 1 cut(s) 939
Sse9I AATT 1 cut(s) 650
SsiI CCGC 6 cut(s) 44, 47, 50, 110, 227, 985
SspMI CTAG 3 cut(s) 126, 285, 447
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 2 cut(s) 269, 967
TaiI ACGT 2 cut(s) 138, 518
TaqI TCGA 2 cut(s) 278, 458
TaqII GACCGA 1 cut(s) 564
TasI AATT 1 cut(s) 650
TatI WGTACW 1 cut(s) 732
TauI GCSGC 3 cut(s) 47, 50, 53
TfiI GAWTC 1 cut(s) 661
Tru1I TTAA 2 cut(s) 396, 1037
Tru9I TTAA 2 cut(s) 396, 1037
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 2 cut(s) 628, 919
TseI GCWGC 5 cut(s) 210, 302, 305, 856, 889
Tsp45I GTSAC 2 cut(s) 628, 919
TspDTI ATGAA 2 cut(s) 959, 967
TspRI CASTG 1 cut(s) 274
XapI RAATTY 1 cut(s) 650
XceI RCATGY 2 cut(s) 692, 809
XcmI CCANNNNNNNNNTGG 1 cut(s) 436
XmaJI CCTAGG 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 257
XspI CTAG 3 cut(s) 126, 285, 447
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.