Rh2DG591800

Transmembrane protein 33 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013573
Physical Location & Seq
Reverse (-)
82437017 .. 82440725
3709 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG591800.1

Sequence Viewer

Length: 1035 bp
ATGGGGGAGGAGAGAGACGACCCACAGAAGCTGAAGAAGATAGCGGCGGCGGCTTACGACTACGAGAACGACCCGAGATGGGCCGACTACTGGGCCAACATCCTCATCCCGCCTCACATGGCCTCGAGCCCTGACGTCATCGGCCATTTCAAGCAGAAGTTCTATAAGCGCTATATTGATCCTGAGCTTCTGGTAGATGCAATGTCCGGCAGCAGTCCTCCTCCTCCGCCAAAGAGGCCATCAGCATCGTCCCGGTCTGCAAATGACAGTGCAGGATCAACTGCTAGAACATCAGGAACAACTGCAGCATCAGGAGCATCAGGATCATCAGCAACAAGCGCTCCAACTTCTCTGCGTTGGGATCACCAAACCATTCAGTTTTCTGTTAATGCTTGGGTGTTTCTTGTGGCTGTGCTTGCCATTTTCCCATTGGTGCCTAGGCACCTTTCGAATAGAGCATATCGGCTTTCTTTTATGGGCACAGCGTGTTCCTCTCTTTATTCCACGTACTCGCTCTATGGGATACCGAGGGACTGGAACTTACAGGGTTTGCAAGTTTACTTTCGGTCAATAGTCATGTCTAAAGATTTTATCTACTTGATATACTGCTTGACCTTTGTCACATCCCATCTTTCTCTCAAATTCGCCTTGATTCCCATTCTATGTCGGGCTCTCGTACATGTTGCCAAGTTCCTTAGGCGCAACTTTAGTCAATCCTCATTGTACAGGAAATACTTGGAAGAGCCTTGCGTTTGGGTTGAGTCAAACACAACTACCCTCAGCATACTCTCTTCACATGCTGAGATTGGAAATGGCTTTCTTTTGATTATATCTTTGTTCTCGTGGCAGCGCAACATTATACAGACATTCGCATATTGGCAGCTATTGAAGCTCATGTATCATGCTCCGGTGACTTCTGACTACCATCTGAGTGTGTGGACTAAGATAGGGAGGACAGTTCATCCATTCATCCACCGCTACGCCCCATTCTTGGAGACACCCGTTTCTACAATCCAGAGATGGTGGTTAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0121

Protein Analysis

344

Amino Acids

39.13

Weight (kDa)

9.53

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM33_Pom33 PF03661 123 - 302 4.2e-14 Transmembrane protein 33/Nucleoporin POM33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02420 AT3G02420
fragaria_vesca FvH4_6g45520 FvH4_6g45520
malus_domestica MD09G1082200.v1.1 MD17G1072900.v1.1
prunus_persica Prupe.3G241500_v2.0.a1
pyrus_communis pycom09g00870 pycom17g07380
rosa_chinensis RchiOBHm_Chr2g0163461
rosa_laevigata RLG00000021403
rosa_multiflora Rmu_sc0012243.1_g000002
rosa_roxburghii Rroxscaffold_2G00087300
rosa_samantha Rh2AG569900 Rh2BG582100 Rh2CG551900 Rh2DG591800
rosa_wichuraiana Rw2G047180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 433, 441
AciI CCGC 6 cut(s) 44, 47, 50, 110, 227, 976
AclWI GGATC 4 cut(s) 173, 283, 331, 369
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 135
AdeI CACNNNGTG 1 cut(s) 486
AfaI GTAC 3 cut(s) 509, 678, 725
AfeI AGCGCT 2 cut(s) 170, 340
AfiI CCNNNNNNNGG 3 cut(s) 79, 90, 991
AflIII ACRYGT 1 cut(s) 679
AgsI TTSAA 2 cut(s) 151, 889
AluBI AGCT 4 cut(s) 31, 187, 883, 892
AluI AGCT 4 cut(s) 31, 187, 883, 892
Alw26I GTCTC 2 cut(s) 9, 989
AlwI GGATC 4 cut(s) 173, 283, 331, 369
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 2 cut(s) 73, 124
Aor51HI AGCGCT 2 cut(s) 170, 340
AoxI GGCC 5 cut(s) 81, 93, 120, 142, 236
ApeKI GCWGC 4 cut(s) 210, 305, 847, 880
ApoI RAATTY 1 cut(s) 641
AspA2I CCTAGG 1 cut(s) 437
AspLEI GCGC 4 cut(s) 171, 341, 702, 852
AspS9I GGNCC 2 cut(s) 81, 93
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 2 cut(s) 356, 922
AsuII TTCGAA 1 cut(s) 449
AvaI CYCGRG 2 cut(s) 73, 124
AvrII CCTAGG 1 cut(s) 437
AxyI CCTNAGG 1 cut(s) 695
BaeGI GKGCMC 1 cut(s) 482
BanI GGYRCC 2 cut(s) 433, 441
BanII GRGCYC 2 cut(s) 131, 673
BauI CACGAG 1 cut(s) 841
BbvCI CCTCAGC 1 cut(s) 779
BbvI GCAGC 4 cut(s) 222, 317, 859, 892
BccI CCATC 5 cut(s) 72, 247, 636, 933, 1014
BciVI GTATCC 1 cut(s) 516
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 9, 989
BfaI CTAG 2 cut(s) 285, 438
BfmI CTRYAG 1 cut(s) 303
BfoI RGCGCY 2 cut(s) 172, 342
BfuI GTATCC 1 cut(s) 516
BglI GCCNNNNNGGC 1 cut(s) 235
BisI GCNGC 7 cut(s) 45, 48, 51, 211, 306, 848, 881
BlnI CCTAGG 1 cut(s) 437
BlsI GCNGC 7 cut(s) 46, 49, 52, 212, 307, 849, 882
Bme1390I CCNGG 1 cut(s) 253
BmeT110I CYCGRG 2 cut(s) 73, 124
BmgT120I GGNCC 2 cut(s) 81, 93
BmiI GGNNCC 2 cut(s) 435, 443
BmrFI CCNGG 1 cut(s) 253
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 4 cut(s) 187, 254, 317, 326
BmuI ACTGGG 1 cut(s) 100
BoxI GACNNNNGTC 1 cut(s) 617
Bpu10I CCTNAGC 2 cut(s) 183, 779
Bpu14I TTCGAA 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 253
BsaAI YACGTR 1 cut(s) 507
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 437, 527
BsaWI WCCGGW 1 cut(s) 907
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 90, 991
Bse1I ACTGG 2 cut(s) 95, 539
Bse21I CCTNAGG 1 cut(s) 695
Bse3DI GCAATG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 437, 527
BseGI GGATG 5 cut(s) 99, 105, 623, 961, 969
BseLI CCNNNNNNNGG 3 cut(s) 79, 90, 991
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 4 cut(s) 174, 792, 793, 920
BseNI ACTGG 2 cut(s) 95, 539
BseRI GAGGAG 3 cut(s) 23, 210, 213
BseSI GKGCMC 1 cut(s) 482
BseXI GCAGC 4 cut(s) 222, 317, 859, 892
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 5 cut(s) 83, 95, 122, 144, 238
BshNI GGYRCC 2 cut(s) 433, 441
BsiHKCI CYCGRG 2 cut(s) 73, 124
BsiSI CCGG 3 cut(s) 207, 253, 908
BslFI GGGAC 2 cut(s) 235, 545
BslI CCNNNNNNNGG 3 cut(s) 79, 90, 991
BsmAI GTCTC 2 cut(s) 9, 989
BsmBI CGTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 235, 545
BsnI GGCC 5 cut(s) 83, 95, 122, 144, 238
BsoBI CYCGRG 2 cut(s) 73, 124
Bsp119I TTCGAA 1 cut(s) 449
Bsp1286I GDGCHC 3 cut(s) 131, 482, 673
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 4 cut(s) 178, 275, 323, 361
BspACI CCGC 6 cut(s) 44, 47, 50, 110, 227, 976
BspANI GGCC 5 cut(s) 83, 95, 122, 144, 238
BspCNI CTCAG 4 cut(s) 175, 792, 793, 921
BspLI GGNNCC 2 cut(s) 435, 443
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 4 cut(s) 173, 283, 331, 369
BspQI GCTCTTC 1 cut(s) 735
BspT104I TTCGAA 1 cut(s) 449
BspT107I GGYRCC 2 cut(s) 433, 441
BsrDI GCAATG 1 cut(s) 207
BsrGI TGTACA 1 cut(s) 723
BsrI ACTGG 2 cut(s) 95, 539
BssECI CCNNGG 2 cut(s) 437, 527
BssMI GATC 4 cut(s) 178, 275, 323, 361
BssNI GRCGYC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 841
BssT1I CCWWGG 1 cut(s) 437
Bst2BI CACGAG 1 cut(s) 841
Bst4CI ACNGT 2 cut(s) 269, 958
Bst6I CTCTTC 2 cut(s) 735, 796
BstACI GRCGYC 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 723
BstBAI YACGTR 1 cut(s) 507
BstBI TTCGAA 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 417
BstDEI CTNAG 6 cut(s) 183, 695, 779, 801, 929, 942
BstF5I GGATG 5 cut(s) 99, 105, 623, 961, 969
BstH2I RGCGCY 2 cut(s) 172, 342
BstHHI GCGC 4 cut(s) 171, 341, 702, 852
BstKTI GATC 4 cut(s) 181, 278, 326, 364
BstMAI GTCTC 2 cut(s) 9, 989
BstMBI GATC 4 cut(s) 178, 275, 323, 361
BstMWI GCNNNNNNNGC 6 cut(s) 50, 235, 314, 338, 416, 889
BstNSI RCATGY 2 cut(s) 683, 800
BstPAI GACNNNNGTC 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 251
BstSFI CTRYAG 1 cut(s) 303
BstSLI GKGCMC 1 cut(s) 482
BstV1I GCAGC 4 cut(s) 222, 317, 859, 892
Bsu36I CCTNAGG 1 cut(s) 695
BsuI GTATCC 1 cut(s) 516
BsuRI GGCC 5 cut(s) 83, 95, 122, 144, 238
BtsCI GGATG 5 cut(s) 99, 105, 623, 961, 969
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 417
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 4 cut(s) 171, 341, 702, 852
Cfr13I GGNCC 2 cut(s) 81, 93
Csp6I GTAC 3 cut(s) 508, 677, 724
CviAII CATG 6 cut(s) 118, 577, 680, 797, 895, 902
CviQI GTAC 3 cut(s) 508, 677, 724
DdeI CTNAG 6 cut(s) 183, 695, 779, 801, 929, 942
DpnI GATC 4 cut(s) 180, 277, 325, 363
DpnII GATC 4 cut(s) 178, 275, 323, 361
DraIII CACNNNGTG 1 cut(s) 486
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 2 cut(s) 735, 796
EarI CTCTTC 2 cut(s) 735, 796
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 437
Eco24I GRGCYC 2 cut(s) 131, 673
Eco47III AGCGCT 2 cut(s) 170, 340
Eco57I CTGAAG 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 695
Eco88I CYCGRG 2 cut(s) 73, 124
EcoT14I CCWWGG 1 cut(s) 437
EcoT38I GRGCYC 2 cut(s) 131, 673
ErhI CCWWGG 1 cut(s) 437
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 6 cut(s) 121, 580, 683, 800, 898, 905
FaqI GGGAC 2 cut(s) 235, 545
FatI CATG 6 cut(s) 117, 576, 679, 796, 894, 901
FauI CCCGC 1 cut(s) 117
Fnu4HI GCNGC 7 cut(s) 45, 48, 51, 211, 306, 848, 881
FokI GGATG 5 cut(s) 86, 92, 610, 948, 956
FriOI GRGCYC 2 cut(s) 131, 673
Fsp4HI GCNGC 7 cut(s) 45, 48, 51, 211, 306, 848, 881
FspBI CTAG 2 cut(s) 285, 438
GlaI GCGC 4 cut(s) 170, 340, 701, 851
GluI GCNGC 7 cut(s) 45, 48, 51, 211, 306, 848, 881
HaeII RGCGCY 2 cut(s) 172, 342
HaeIII GGCC 5 cut(s) 83, 95, 122, 144, 238
HapII CCGG 3 cut(s) 207, 253, 908
HhaI GCGC 4 cut(s) 171, 341, 702, 852
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 6 cut(s) 121, 580, 683, 800, 898, 905
Hin6I GCGC 4 cut(s) 169, 339, 700, 850
HinP1I GCGC 4 cut(s) 169, 339, 700, 850
HinfI GANTC 2 cut(s) 652, 761
HpaII CCGG 3 cut(s) 207, 253, 908
HphI GGTGA 2 cut(s) 356, 922
Hpy166II GTNNAC 2 cut(s) 559, 939
Hpy188I TCNGA 2 cut(s) 919, 930
Hpy188III TCNNGA 5 cut(s) 182, 294, 312, 321, 1015
Hpy8I GTNNAC 2 cut(s) 559, 939
HpyCH4III ACNGT 2 cut(s) 269, 958
HpyCH4IV ACGT 2 cut(s) 135, 506
HpyCH4V TGCA 5 cut(s) 200, 260, 272, 305, 553
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 235, 314, 338, 416, 889
HpyF3I CTNAG 6 cut(s) 183, 695, 779, 801, 929, 942
HpySE526I ACGT 2 cut(s) 135, 506
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 6 cut(s) 121, 580, 683, 800, 898, 905
HspAI GCGC 4 cut(s) 169, 339, 700, 850
Kzo9I GATC 4 cut(s) 178, 275, 323, 361
LguI GCTCTTC 1 cut(s) 735
LmnI GCTCC 3 cut(s) 314, 346, 910
Lsp1109I GCAGC 4 cut(s) 222, 317, 859, 892
LweI GCATC 4 cut(s) 187, 254, 317, 326
MaeI CTAG 2 cut(s) 285, 438
MaeII ACGT 2 cut(s) 135, 506
MaeIII GTNAC 2 cut(s) 619, 910
MalI GATC 4 cut(s) 180, 277, 325, 363
MboI GATC 4 cut(s) 178, 275, 323, 361
MboII GAAGA 4 cut(s) 46, 49, 752, 783
MhlI GDGCHC 3 cut(s) 131, 482, 673
MluCI AATT 1 cut(s) 641
MlyI GAGTC 1 cut(s) 770
MmeI TCCRAC 1 cut(s) 368
MseI TTAA 2 cut(s) 387, 1028
MslI CAYNNNNRTG 1 cut(s) 930
MspI CCGG 3 cut(s) 207, 253, 908
MspR9I CCNGG 1 cut(s) 253
MwoI GCNNNNNNNGC 6 cut(s) 50, 235, 314, 338, 416, 889
NciI CCSGG 1 cut(s) 253
NdeII GATC 4 cut(s) 178, 275, 323, 361
NlaIII CATG 6 cut(s) 121, 580, 683, 800, 898, 905
NlaIV GGNNCC 2 cut(s) 435, 443
NmuCI GTSAC 2 cut(s) 619, 910
NspI RCATGY 2 cut(s) 683, 800
NspV TTCGAA 1 cut(s) 449
PaeR7I CTCGAG 1 cut(s) 124
PciI ACATGT 1 cut(s) 679
PciSI GCTCTTC 1 cut(s) 735
PfeI GAWTC 1 cut(s) 652
PkrI GCNGC 7 cut(s) 46, 49, 52, 212, 307, 849, 882
PleI GAGTC 1 cut(s) 769
PpsI GAGTC 1 cut(s) 769
Ppu21I YACGTR 1 cut(s) 507
PscI ACATGT 1 cut(s) 679
PshAI GACNNNNGTC 1 cut(s) 617
PspN4I GGNNCC 2 cut(s) 435, 443
PspPI GGNCC 2 cut(s) 81, 93
PspXI VCTCGAGB 1 cut(s) 124
PstI CTGCAG 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 31
RsaI GTAC 3 cut(s) 509, 678, 725
RsaNI GTAC 3 cut(s) 508, 677, 724
RseI CAYNNNNRTG 1 cut(s) 930
SapI GCTCTTC 1 cut(s) 735
SaqAI TTAA 2 cut(s) 387, 1028
SatI GCNGC 7 cut(s) 45, 48, 51, 211, 306, 848, 881
Sau3AI GATC 4 cut(s) 178, 275, 323, 361
Sau96I GGNCC 2 cut(s) 81, 93
SchI GAGTC 1 cut(s) 770
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 3 cut(s) 131, 482, 673
SetI ASST 9 cut(s) 33, 138, 189, 447, 509, 617, 885, 894, 1034
SfaNI GCATC 4 cut(s) 187, 254, 317, 326
SfcI CTRYAG 1 cut(s) 303
Sfr274I CTCGAG 1 cut(s) 124
SfuI TTCGAA 1 cut(s) 449
SlaI CTCGAG 1 cut(s) 124
SmiMI CAYNNNNRTG 1 cut(s) 930
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 1 cut(s) 641
SsiI CCGC 6 cut(s) 44, 47, 50, 110, 227, 976
SspMI CTAG 2 cut(s) 285, 438
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 437
TaaI ACNGT 2 cut(s) 269, 958
TaiI ACGT 2 cut(s) 138, 509
TaqI TCGA 2 cut(s) 125, 449
TaqII GACCGA 1 cut(s) 555
TasI AATT 1 cut(s) 641
TatI WGTACW 1 cut(s) 723
TauI GCSGC 3 cut(s) 47, 50, 53
TfiI GAWTC 1 cut(s) 652
Tru1I TTAA 2 cut(s) 387, 1028
Tru9I TTAA 2 cut(s) 387, 1028
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 2 cut(s) 619, 910
TseI GCWGC 4 cut(s) 210, 305, 847, 880
Tsp45I GTSAC 2 cut(s) 619, 910
TspDTI ATGAA 2 cut(s) 950, 958
TspRI CASTG 1 cut(s) 274
XapI RAATTY 1 cut(s) 641
XceI RCATGY 2 cut(s) 683, 800
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XhoI CTCGAG 1 cut(s) 124
XmaJI CCTAGG 1 cut(s) 437
XspI CTAG 2 cut(s) 285, 438
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.