RLG00000021403

Transmembrane protein 33 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013573
Physical Location & Seq
Reverse (-)
74879314 .. 74883357
4044 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021403

Sequence Viewer

Length: 732 bp
ATGGGGGAGGAGAGAGACGACCCACAGAAGCTGAAGAAGATAGCGGCGGCGGCTTACGACTACGAGAACGACCCGAGATGGGCCGACTACTGGGCCAACATCCTCATCCCTCCTCACATGGCCTCTAGCTCTGACGTCATCAGCCATTTCAAGCAGAAGTTCTATAAGCGCTATATTGATCCTGAGCTTCTGGTAGATGCAATGTCCGGCAGCAGTCCTCCTCCTCTGCCAAAGAGGCCATCAGCATCGTCCCGGTCTACAAATGACAGTGCAGGATCAACTGCTAGAACGTCAGGAACAACAGCAGCATCAGGAACATCAGGATCATCAGCAACAAGCGCTCCAACTTCTCTGCGTTGGGATCACCAAACCATTCAGTTTTCTGTTAATGCTTGGGTGTTTCTTGTGGCTGTGCTTGCCATTTTCCCATTGGTGCCTAGGCACCTTTCGAATAGAGCATATCGGCTTTCTTTAATGGGCACAGCGTGTTCCTCTCTTTATTCCATGTACTCGCTCTATGGGAAACCGAGGGACTGGAACTTACAGGGTTTGCAAGTTTACTTTCGGTCAATAGTCATGGCTAAAGATTTTATCTACTTGATATACTGCTTGACCTTTGTCACATCCCATCTTTCTCTCAAATTCGCCTTGATTCCCATTCTATGTCGGGCTCTCGTACACGTTGCCAAGTTCCTTAGGCGCAACTTTAGTCAATCCTCATTGTACAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0121

Protein Analysis

244

Amino Acids

27.25

Weight (kDa)

9.66

Isoelectric Point (pI)

50.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM33_Pom33 PF03661 123 - 234 8.3e-08 Transmembrane protein 33/Nucleoporin POM33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02420 AT3G02420
fragaria_vesca FvH4_6g45520 FvH4_6g45520
malus_domestica MD09G1082200.v1.1 MD17G1072900.v1.1
prunus_persica Prupe.3G241500_v2.0.a1
pyrus_communis pycom09g00870 pycom17g07380
rosa_chinensis RchiOBHm_Chr2g0163461
rosa_laevigata RLG00000021403
rosa_multiflora Rmu_sc0012243.1_g000002
rosa_roxburghii Rroxscaffold_2G00087300
rosa_samantha Rh2AG569900 Rh2BG582100 Rh2CG551900 Rh2DG591800
rosa_wichuraiana Rw2G047180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 433, 441
AccI GTMKAC 1 cut(s) 257
AciI CCGC 3 cut(s) 44, 47, 50
AclWI GGATC 4 cut(s) 173, 283, 331, 369
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 135
AdeI CACNNNGTG 1 cut(s) 486
AfaI GTAC 3 cut(s) 509, 678, 725
AfeI AGCGCT 2 cut(s) 170, 340
AfiI CCNNNNNNNGG 2 cut(s) 79, 90
AflIII ACRYGT 1 cut(s) 679
AgsI TTSAA 1 cut(s) 151
AluBI AGCT 3 cut(s) 31, 129, 187
AluI AGCT 3 cut(s) 31, 129, 187
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 4 cut(s) 173, 283, 331, 369
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 73
Aor51HI AGCGCT 2 cut(s) 170, 340
AoxI GGCC 4 cut(s) 81, 93, 120, 236
ApeKI GCWGC 2 cut(s) 210, 305
ApoI RAATTY 1 cut(s) 641
AspA2I CCTAGG 1 cut(s) 437
AspLEI GCGC 3 cut(s) 171, 341, 702
AspS9I GGNCC 2 cut(s) 81, 93
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 356
AsuII TTCGAA 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 73
AvrII CCTAGG 1 cut(s) 437
AxyI CCTNAGG 1 cut(s) 695
BaeGI GKGCMC 1 cut(s) 482
BanI GGYRCC 2 cut(s) 433, 441
BanII GRGCYC 1 cut(s) 673
BbvI GCAGC 2 cut(s) 222, 317
BccI CCATC 3 cut(s) 72, 247, 636
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 3 cut(s) 126, 285, 438
BfoI RGCGCY 2 cut(s) 172, 342
BglI GCCNNNNNGGC 1 cut(s) 235
BisI GCNGC 5 cut(s) 45, 48, 51, 211, 306
BlnI CCTAGG 1 cut(s) 437
BlsI GCNGC 5 cut(s) 46, 49, 52, 212, 307
Bme1390I CCNGG 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 2 cut(s) 81, 93
BmiI GGNNCC 2 cut(s) 435, 443
BmrFI CCNGG 1 cut(s) 253
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 3 cut(s) 187, 254, 317
BmuI ACTGGG 1 cut(s) 100
BoxI GACNNNNGTC 1 cut(s) 617
Bpu10I CCTNAGC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 253
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 437, 527
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 90
Bse1I ACTGG 2 cut(s) 95, 539
Bse21I CCTNAGG 1 cut(s) 695
Bse3DI GCAATG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 437, 527
BseGI GGATG 3 cut(s) 99, 105, 623
BseLI CCNNNNNNNGG 2 cut(s) 79, 90
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 2 cut(s) 95, 539
BseRI GAGGAG 4 cut(s) 23, 102, 210, 213
BseSI GKGCMC 1 cut(s) 482
BseXI GCAGC 2 cut(s) 222, 317
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 4 cut(s) 83, 95, 122, 238
BshNI GGYRCC 2 cut(s) 433, 441
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 2 cut(s) 207, 253
BslFI GGGAC 2 cut(s) 235, 545
BslI CCNNNNNNNGG 2 cut(s) 79, 90
BsmAI GTCTC 1 cut(s) 9
BsmBI CGTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 235, 545
BsnI GGCC 4 cut(s) 83, 95, 122, 238
BsoBI CYCGRG 1 cut(s) 73
Bsp119I TTCGAA 1 cut(s) 449
Bsp1286I GDGCHC 2 cut(s) 482, 673
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 4 cut(s) 178, 275, 323, 361
BspACI CCGC 3 cut(s) 44, 47, 50
BspANI GGCC 4 cut(s) 83, 95, 122, 238
BspCNI CTCAG 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 435, 443
BspPI GGATC 4 cut(s) 173, 283, 331, 369
BspT104I TTCGAA 1 cut(s) 449
BspT107I GGYRCC 2 cut(s) 433, 441
BsrDI GCAATG 1 cut(s) 207
BsrGI TGTACA 1 cut(s) 723
BsrI ACTGG 2 cut(s) 95, 539
BssECI CCNNGG 2 cut(s) 437, 527
BssMI GATC 4 cut(s) 178, 275, 323, 361
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 437
Bst4CI ACNGT 1 cut(s) 269
BstACI GRCGYC 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 723
BstBI TTCGAA 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 417
BstDEI CTNAG 2 cut(s) 183, 695
BstF5I GGATG 3 cut(s) 99, 105, 623
BstH2I RGCGCY 2 cut(s) 172, 342
BstHHI GCGC 3 cut(s) 171, 341, 702
BstKTI GATC 4 cut(s) 181, 278, 326, 364
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 178, 275, 323, 361
BstMWI GCNNNNNNNGC 4 cut(s) 50, 235, 338, 416
BstPAI GACNNNNGTC 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 251
BstSLI GKGCMC 1 cut(s) 482
BstV1I GCAGC 2 cut(s) 222, 317
Bsu36I CCTNAGG 1 cut(s) 695
BsuRI GGCC 4 cut(s) 83, 95, 122, 238
BtsCI GGATG 3 cut(s) 99, 105, 623
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 417
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 3 cut(s) 171, 341, 702
Cfr13I GGNCC 2 cut(s) 81, 93
Csp6I GTAC 3 cut(s) 508, 677, 724
CviAII CATG 3 cut(s) 118, 505, 577
CviQI GTAC 3 cut(s) 508, 677, 724
DdeI CTNAG 2 cut(s) 183, 695
DpnI GATC 4 cut(s) 180, 277, 325, 363
DpnII GATC 4 cut(s) 178, 275, 323, 361
DraIII CACNNNGTG 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 673
Eco47III AGCGCT 2 cut(s) 170, 340
Eco57I CTGAAG 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 695
Eco88I CYCGRG 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 437
EcoT38I GRGCYC 1 cut(s) 673
ErhI CCWWGG 1 cut(s) 437
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 3 cut(s) 121, 508, 580
FaiI YATR 9 cut(s) 119, 165, 174, 460, 506, 519, 578, 604, 664
FaqI GGGAC 2 cut(s) 235, 545
FatI CATG 3 cut(s) 117, 504, 576
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 5 cut(s) 45, 48, 51, 211, 306
FokI GGATG 3 cut(s) 86, 92, 610
FriOI GRGCYC 1 cut(s) 673
Fsp4HI GCNGC 5 cut(s) 45, 48, 51, 211, 306
FspBI CTAG 3 cut(s) 126, 285, 438
GlaI GCGC 3 cut(s) 170, 340, 701
GluI GCNGC 5 cut(s) 45, 48, 51, 211, 306
HaeII RGCGCY 2 cut(s) 172, 342
HaeIII GGCC 4 cut(s) 83, 95, 122, 238
HapII CCGG 2 cut(s) 207, 253
HhaI GCGC 3 cut(s) 171, 341, 702
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 3 cut(s) 121, 508, 580
Hin6I GCGC 3 cut(s) 169, 339, 700
HinP1I GCGC 3 cut(s) 169, 339, 700
HinfI GANTC 1 cut(s) 652
HpaII CCGG 2 cut(s) 207, 253
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 3 cut(s) 258, 559, 679
Hpy188I TCNGA 1 cut(s) 133
Hpy188III TCNNGA 4 cut(s) 182, 294, 312, 321
Hpy8I GTNNAC 3 cut(s) 258, 559, 679
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 3 cut(s) 135, 290, 681
HpyCH4V TGCA 3 cut(s) 200, 272, 553
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 235, 338, 416
HpyF3I CTNAG 2 cut(s) 183, 695
HpySE526I ACGT 3 cut(s) 135, 290, 681
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 3 cut(s) 121, 508, 580
HspAI GCGC 3 cut(s) 169, 339, 700
Kzo9I GATC 4 cut(s) 178, 275, 323, 361
LmnI GCTCC 1 cut(s) 346
Lsp1109I GCAGC 2 cut(s) 222, 317
LweI GCATC 3 cut(s) 187, 254, 317
MaeI CTAG 3 cut(s) 126, 285, 438
MaeII ACGT 3 cut(s) 135, 290, 681
MaeIII GTNAC 1 cut(s) 619
MalI GATC 4 cut(s) 180, 277, 325, 363
MboI GATC 4 cut(s) 178, 275, 323, 361
MboII GAAGA 2 cut(s) 46, 49
MhlI GDGCHC 2 cut(s) 482, 673
MluCI AATT 1 cut(s) 641
MmeI TCCRAC 1 cut(s) 368
MseI TTAA 2 cut(s) 387, 473
MspI CCGG 2 cut(s) 207, 253
MspR9I CCNGG 1 cut(s) 253
MwoI GCNNNNNNNGC 4 cut(s) 50, 235, 338, 416
NciI CCSGG 1 cut(s) 253
NdeII GATC 4 cut(s) 178, 275, 323, 361
NlaIII CATG 3 cut(s) 121, 508, 580
NlaIV GGNNCC 2 cut(s) 435, 443
NmuCI GTSAC 1 cut(s) 619
NspV TTCGAA 1 cut(s) 449
PfeI GAWTC 1 cut(s) 652
PkrI GCNGC 5 cut(s) 46, 49, 52, 212, 307
PshAI GACNNNNGTC 1 cut(s) 617
PspN4I GGNNCC 2 cut(s) 435, 443
PspPI GGNCC 2 cut(s) 81, 93
PstNI CAGNNNCTG 1 cut(s) 31
RsaI GTAC 3 cut(s) 509, 678, 725
RsaNI GTAC 3 cut(s) 508, 677, 724
SaqAI TTAA 2 cut(s) 387, 473
SatI GCNGC 5 cut(s) 45, 48, 51, 211, 306
Sau3AI GATC 4 cut(s) 178, 275, 323, 361
Sau96I GGNCC 2 cut(s) 81, 93
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 2 cut(s) 482, 673
SetI ASST 9 cut(s) 33, 131, 138, 189, 293, 447, 617, 684, 731
SfaNI GCATC 3 cut(s) 187, 254, 317
SfuI TTCGAA 1 cut(s) 449
Sse9I AATT 1 cut(s) 641
SsiI CCGC 3 cut(s) 44, 47, 50
SspMI CTAG 3 cut(s) 126, 285, 438
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 437
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 3 cut(s) 138, 293, 684
TaqI TCGA 1 cut(s) 449
TaqII GACCGA 1 cut(s) 555
TasI AATT 1 cut(s) 641
TatI WGTACW 2 cut(s) 507, 723
TauI GCSGC 3 cut(s) 47, 50, 53
TfiI GAWTC 1 cut(s) 652
Tru1I TTAA 2 cut(s) 387, 473
Tru9I TTAA 2 cut(s) 387, 473
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 2 cut(s) 210, 305
Tsp45I GTSAC 1 cut(s) 619
TspRI CASTG 1 cut(s) 274
XapI RAATTY 1 cut(s) 641
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XmaJI CCTAGG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 257
XspI CTAG 3 cut(s) 126, 285, 438
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.