FvH4_6g47500

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005380
Physical Location & Seq
Reverse (-)
35963343 .. 35967010
3668 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47500.t3

Sequence Viewer

Length: 1815 bp
ATGGCTGATTCAAGTATCAGTGTACCAATGGCCCAAGAATTAACAAAGCCAGATGTGGGTAACTTGAGACGAAATTCTACAGGAAAGATACTATCATCAAACAGCGTAGAAAAAGTTCCTCATTATCTCAGGGCTTCCACTGGTTCCTGTCATGATTTTTGCAAGTATGGGAGGAAAGATGCATTTGAAGAGAAGGAAAGATGTCCGATACGGGTTGCACCAAGAAGACTCTCAACTATATCATTTGATAGCCATAACTCAGCAGACAGTGTGGTTTTGCCAGAGAGGAAGAATATATCAGTAATCAAGCTCAATCATTCACATGAGTCCAAAACCTTCTTACCTGATACAGGTACCATCACCAAGCAGGCCAAGCAGCAATTGCCAAAAAGGTCAGCTGACAGAAAAGATGGTGTTGGGAGTGAGCTTCTTACTGAGAGGAAGAAGCTATCATTGGTAAAGTTCAAAACGTCATCCGATTCAAAACCTTCTTTATCTTGTGTGCCCAAAACTGTGAAGCAAGAAGTTTCATCATCTCCTGAAAAGCTGAAAGTTTCTGCAAAGAAAGGTTGGACAAAACTTAAGGAGAAAGACTTGTCTACAAAACATGTCACTTCTTCGAAGCCAAAATCTTTAGCCACGAAGGAATTGTCATCTACTGATACTTCAGGAGGTTCGAAGGGGATAAGAAACAGTAATTCAAAGGAAAGCCAGAGGACAGTCACCTCTCTGAAGCCAAACTCTTTGGCTGCAAACCAAATTTCTTCTCTTGACTCTTCAGGAGGCTTAAATGGCCTCAGGAAAAGTGAGGTGAAGATGGGCAAGAAGGCAGGAACATCCTTAGTCACTTTAAAGAAAGTATTGGTGCCCCCAACAGGATCATTGTCCCCAAAACCTTCTCTCAGGAGAGTTGCAAGCTTAAAAGCACAGAACAGGAATGTGAAAGTTACTCCTCTTAAGAATCAGAACAAGATTGGGAAAGTCATAGCTAAGCAACTCGACAATGATATGACTCCAGAGAAGACCTTGTATGTCATTAAGATTGAGACTGAGAATAAGCCTCTGGAATCTGATCAAGACAAAAATTGTGAAGGTCCATCACCACCTTCATCGTCCCCATCATCGCCCAAGTCTGTGTCCCTCCCAAACAGCATGCCACTTTCACCCCATGACGGAGACAAAAATTGTGAAGGTCCATCACCACCTTCATCGTCCCCATCATCGCCCAAGTCTGTGTCCCTCCCAAACAGCATGCCACTTTCACCCCATGACGGAGAAGATCAAGAGTCTGAATATACAATGACGGAAACTGAAGAGGACTCTTTCTCAGAAGACGGTGAAGTTGACAGTGAAGAAAATGCAGAGACCCTAAATGAGGATTACAAAGGGAGGCCCAGAAAGTCTGGGATCGTTTGTTCTGAAGATGTGGATAGCCATCCTCTGAAGTTTAGGAGGGGAAAGGTGGTTGACATGCAATTCGAGAATAATAGACCAAGGAGGCTCAAATTCAGGCGAGGAAAAGTGTTGGGAGAGAACGAAAACATCAAGTCTGATAACCAAAGGCGAAGATATAAGAAGAGAGAAGGTGTTGATGGCGATGCAATTGGTACCGATGCTGGTGCAGAAAAAGTTGTTTTGAGACATCAAGATGTTCAGGGGAAGAAAGATGAGAAGGGGTTGTTTAACAACGTAATTGAAGAAACTGCAAGTAAACTGGTTGAAACCCAGAAGAGTAAAGTCAAGGCCTTAGTTGGTGCTTTTGAAACAGTGATCTCACTTCAAGAGCGCAAACCTTCTGCAAATTCTGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

605

Amino Acids

66.11

Weight (kDa)

9.52

Isoelectric Point (pI)

53.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 448 - 559 2e-35 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 353, 1607
AccB1I GGYRCC 3 cut(s) 353, 865, 1607
AccI GTMKAC 1 cut(s) 599
AclWI GGATC 2 cut(s) 886, 1415
AcsI RAATTY 4 cut(s) 73, 759, 1505, 1801
AcuI CTGAAG 6 cut(s) 651, 752, 762, 1332, 1440, 1463
AfaI GTAC 3 cut(s) 24, 355, 1609
AfiI CCNNNNNNNGG 6 cut(s) 56, 350, 875, 1172, 1271, 1375
AflII CTTAAG 2 cut(s) 581, 956
AflIII ACRYGT 1 cut(s) 607
AgsI TTSAA 9 cut(s) 12, 188, 466, 483, 702, 1697, 1721, 1763, 1781
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AloI GAACNNNNNNTCC 2 cut(s) 1399, 1431
AluBI AGCT 7 cut(s) 310, 398, 427, 448, 547, 918, 989
AluI AGCT 7 cut(s) 310, 398, 427, 448, 547, 918, 989
Alw26I GTCTC 5 cut(s) 61, 1040, 1170, 1358, 1633
AlwI GGATC 2 cut(s) 886, 1415
AoxI GGCC 5 cut(s) 30, 369, 793, 1391, 1743
ApeKI GCWGC 2 cut(s) 376, 749
ApoI RAATTY 4 cut(s) 73, 759, 1505, 1801
ArsI GACNNNNNNTTYG 4 cut(s) 635, 667, 1460, 1492
Asp700I GAANNNNTTC 1 cut(s) 114
Asp718I GGTACC 2 cut(s) 353, 1607
AspLEI GCGC 1 cut(s) 1788
AspS9I GGNCC 4 cut(s) 31, 1094, 1193, 1392
AsuHPI GGTGA 8 cut(s) 352, 715, 823, 1092, 1155, 1191, 1254, 1349
AsuII TTCGAA 2 cut(s) 620, 677
AvaII GGWCC 2 cut(s) 1094, 1193
AxyI CCTNAGG 1 cut(s) 797
BaeGI GKGCMC 2 cut(s) 507, 870
BanI GGYRCC 3 cut(s) 353, 865, 1607
BbsI GAAGAC 3 cut(s) 232, 1028, 1338
BbvI GCAGC 2 cut(s) 388, 736
BccI CCATC 9 cut(s) 365, 404, 811, 1105, 1126, 1204, 1225, 1443, 1586
BcgI CGANNNNNNTGC 2 cut(s) 1601, 1635
BclI TGATCA 1 cut(s) 1072
BcoDI GTCTC 5 cut(s) 61, 1040, 1170, 1358, 1633
BfmI CTRYAG 1 cut(s) 78
BfrI CTTAAG 2 cut(s) 581, 956
BisI GCNGC 2 cut(s) 377, 750
BlpI GCTNAGC 1 cut(s) 990
BlsI GCNGC 2 cut(s) 378, 751
Bme18I GGWCC 2 cut(s) 1094, 1193
BmgT120I GGNCC 4 cut(s) 31, 1094, 1193, 1392
BmiI GGNNCC 4 cut(s) 145, 355, 867, 1609
BmsI GCATC 3 cut(s) 169, 1588, 1603
BpiI GAAGAC 3 cut(s) 232, 1028, 1338
BpmI CTGGAG 1 cut(s) 999
Bpu1102I GCTNAGC 1 cut(s) 990
Bpu14I TTCGAA 2 cut(s) 620, 677
BpuEI CTTGAG 1 cut(s) 85
BsaBI GATNNNNATC 1 cut(s) 1434
BsaI GGTCTC 1 cut(s) 1358
BsaJI CCNNGG 1 cut(s) 1493
Bsc4I CCNNNNNNNGG 6 cut(s) 56, 350, 875, 1172, 1271, 1375
Bse1I ACTGG 2 cut(s) 145, 1719
Bse21I CCTNAGG 1 cut(s) 797
Bse8I GATNNNNATC 1 cut(s) 1434
BseDI CCNNGG 1 cut(s) 1493
BseGI GGATG 3 cut(s) 473, 836, 1435
BseJI GATNNNNATC 1 cut(s) 1434
BseLI CCNNNNNNNGG 6 cut(s) 56, 350, 875, 1172, 1271, 1375
BseMII CTCAG 7 cut(s) 142, 273, 426, 811, 916, 1041, 1341
BseNI ACTGG 2 cut(s) 145, 1719
BseRI GAGGAG 1 cut(s) 942
BseSI GKGCMC 2 cut(s) 507, 870
BseXI GCAGC 2 cut(s) 388, 736
BsgI GTGCAG 1 cut(s) 1641
BshFI GGCC 5 cut(s) 32, 371, 795, 1393, 1745
BshNI GGYRCC 3 cut(s) 353, 865, 1607
BslFI GGGAC 5 cut(s) 871, 1099, 1123, 1198, 1222
BslI CCNNNNNNNGG 6 cut(s) 56, 350, 875, 1172, 1271, 1375
BsmAI GTCTC 5 cut(s) 61, 1040, 1170, 1358, 1633
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 5 cut(s) 871, 1099, 1123, 1198, 1222
BsnI GGCC 5 cut(s) 32, 371, 795, 1393, 1745
Bso31I GGTCTC 1 cut(s) 1358
Bsp119I TTCGAA 2 cut(s) 620, 677
Bsp1286I GDGCHC 2 cut(s) 507, 870
Bsp143I GATC 5 cut(s) 878, 1072, 1279, 1407, 1770
Bsp1720I GCTNAGC 1 cut(s) 990
BspANI GGCC 5 cut(s) 32, 371, 795, 1393, 1745
BspCNI CTCAG 7 cut(s) 141, 272, 427, 810, 915, 1042, 1340
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 4 cut(s) 145, 355, 867, 1609
BspPI GGATC 2 cut(s) 886, 1415
BspT104I TTCGAA 2 cut(s) 620, 677
BspT107I GGYRCC 3 cut(s) 353, 865, 1607
BspTI CTTAAG 2 cut(s) 581, 956
BspTNI GGTCTC 1 cut(s) 1358
BsrI ACTGG 2 cut(s) 145, 1719
BssECI CCNNGG 1 cut(s) 1493
BssMI GATC 5 cut(s) 878, 1072, 1279, 1407, 1770
BssT1I CCWWGG 1 cut(s) 1493
Bst4CI ACNGT 7 cut(s) 269, 514, 695, 721, 1337, 1349, 1768
Bst6I CTCTTC 5 cut(s) 183, 781, 1308, 1571, 1724
BstAFI CTTAAG 2 cut(s) 581, 956
BstAPI GCANNNNNTGC 1 cut(s) 382
BstBI TTCGAA 2 cut(s) 620, 677
BstC8I GCNNGC 4 cut(s) 369, 916, 1154, 1253
BstENI CCTNNNNNAGG 2 cut(s) 348, 1373
BstF5I GGATG 3 cut(s) 473, 836, 1435
BstHHI GCGC 1 cut(s) 1788
BstKTI GATC 5 cut(s) 881, 1075, 1282, 1410, 1773
BstMAI GTCTC 5 cut(s) 61, 1040, 1170, 1358, 1633
BstMBI GATC 5 cut(s) 878, 1072, 1279, 1407, 1770
BstMWI GCNNNNNNNGC 3 cut(s) 373, 382, 792
BstNSI RCATGY 4 cut(s) 611, 1156, 1255, 1474
BstSFI CTRYAG 1 cut(s) 78
BstSLI GKGCMC 2 cut(s) 507, 870
BstV1I GCAGC 2 cut(s) 388, 736
BstV2I GAAGAC 3 cut(s) 232, 1028, 1338
Bsu36I CCTNAGG 1 cut(s) 797
BsuRI GGCC 5 cut(s) 32, 371, 795, 1393, 1745
BtgZI GCGATG 3 cut(s) 1107, 1206, 1611
BtsCI GGATG 3 cut(s) 473, 836, 1435
BtsIMutI CAGTG 5 cut(s) 25, 138, 274, 1354, 1773
Cac8I GCNNGC 4 cut(s) 369, 916, 1154, 1253
CciI TCATGA 1 cut(s) 151
CfoI GCGC 1 cut(s) 1788
Cfr13I GGNCC 4 cut(s) 31, 1094, 1193, 1392
Csp6I GTAC 3 cut(s) 23, 354, 1608
CviAII CATG 8 cut(s) 152, 323, 608, 1153, 1169, 1252, 1268, 1471
CviQI GTAC 3 cut(s) 23, 354, 1608
DpnI GATC 5 cut(s) 880, 1074, 1281, 1409, 1772
DpnII GATC 5 cut(s) 878, 1072, 1279, 1407, 1770
DraI TTTAAA 1 cut(s) 852
Eam1104I CTCTTC 5 cut(s) 183, 781, 1308, 1571, 1724
EarI CTCTTC 5 cut(s) 183, 781, 1308, 1571, 1724
Eco130I CCWWGG 1 cut(s) 1493
Eco147I AGGCCT 1 cut(s) 1745
Eco31I GGTCTC 1 cut(s) 1358
Eco47I GGWCC 2 cut(s) 1094, 1193
Eco57I CTGAAG 6 cut(s) 651, 752, 762, 1332, 1440, 1463
Eco81I CCTNAGG 1 cut(s) 797
EcoNI CCTNNNNNAGG 2 cut(s) 348, 1373
EcoT14I CCWWGG 1 cut(s) 1493
EcoT22I ATGCAT 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 1493
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 8 cut(s) 155, 326, 611, 1156, 1172, 1255, 1271, 1474
FaqI GGGAC 5 cut(s) 871, 1099, 1123, 1198, 1222
FatI CATG 8 cut(s) 151, 322, 607, 1152, 1168, 1251, 1267, 1470
FbaI TGATCA 1 cut(s) 1072
FblI GTMKAC 1 cut(s) 599
Fnu4HI GCNGC 2 cut(s) 377, 750
FokI GGATG 3 cut(s) 460, 823, 1422
Fsp4HI GCNGC 2 cut(s) 377, 750
GlaI GCGC 1 cut(s) 1787
GluI GCNGC 2 cut(s) 377, 750
GsuI CTGGAG 1 cut(s) 999
HaeIII GGCC 5 cut(s) 32, 371, 795, 1393, 1745
HhaI GCGC 1 cut(s) 1788
Hin1II CATG 8 cut(s) 155, 326, 611, 1156, 1172, 1255, 1271, 1474
Hin6I GCGC 1 cut(s) 1786
HinP1I GCGC 1 cut(s) 1786
HincII GTYRAC 2 cut(s) 1345, 1468
HindII GTYRAC 2 cut(s) 1345, 1468
HindIII AAGCTT 1 cut(s) 916
HphI GGTGA 8 cut(s) 352, 715, 823, 1092, 1155, 1191, 1254, 1349
Hpy166II GTNNAC 5 cut(s) 23, 600, 1345, 1468, 1712
Hpy8I GTNNAC 5 cut(s) 23, 600, 1345, 1468, 1712
HpyCH4III ACNGT 7 cut(s) 269, 514, 695, 721, 1337, 1349, 1768
HpyCH4IV ACGT 2 cut(s) 470, 1689
HpyF10VI GCNNNNNNNGC 3 cut(s) 373, 382, 792
HpySE526I ACGT 2 cut(s) 470, 1689
Hsp92II CATG 8 cut(s) 155, 326, 611, 1156, 1172, 1255, 1271, 1474
HspAI GCGC 1 cut(s) 1786
KpnI GGTACC 2 cut(s) 357, 1611
Ksp22I TGATCA 1 cut(s) 1072
Kzo9I GATC 5 cut(s) 878, 1072, 1279, 1407, 1770
Lsp1109I GCAGC 2 cut(s) 388, 736
LweI GCATC 3 cut(s) 169, 1588, 1603
MaeII ACGT 2 cut(s) 470, 1689
MaeIII GTNAC 5 cut(s) 59, 610, 721, 844, 946
MalI GATC 5 cut(s) 880, 1074, 1281, 1409, 1772
MboI GATC 5 cut(s) 878, 1072, 1279, 1407, 1770
MfeI CAATTG 2 cut(s) 380, 1602
MhlI GDGCHC 2 cut(s) 507, 870
MlyI GAGTC 6 cut(s) 222, 335, 767, 1006, 1295, 1313
MmeI TCCRAC 1 cut(s) 551
Mph1103I ATGCAT 1 cut(s) 184
MroXI GAANNNNTTC 1 cut(s) 114
MseI TTAA 8 cut(s) 41, 582, 788, 851, 920, 957, 1038, 1683
MslI CAYNNNNRTG 2 cut(s) 321, 1647
MspA1I CMGCKG 1 cut(s) 398
MspCI CTTAAG 2 cut(s) 581, 956
MunI CAATTG 2 cut(s) 380, 1602
MwoI GCNNNNNNNGC 3 cut(s) 373, 382, 792
NdeII GATC 5 cut(s) 878, 1072, 1279, 1407, 1770
NlaIII CATG 8 cut(s) 155, 326, 611, 1156, 1172, 1255, 1271, 1474
NlaIV GGNNCC 4 cut(s) 145, 355, 867, 1609
NmuCI GTSAC 3 cut(s) 610, 721, 844
NsiI ATGCAT 1 cut(s) 184
NspI RCATGY 4 cut(s) 611, 1156, 1255, 1474
NspV TTCGAA 2 cut(s) 620, 677
PaeI GCATGC 2 cut(s) 1156, 1255
PagI TCATGA 1 cut(s) 151
PceI AGGCCT 1 cut(s) 1745
PciI ACATGT 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 4 cut(s) 8, 479, 961, 1067
PkrI GCNGC 2 cut(s) 378, 751
PleI GAGTC 6 cut(s) 222, 334, 767, 1006, 1294, 1313
PpsI GAGTC 6 cut(s) 222, 334, 767, 1006, 1294, 1313
PscI ACATGT 1 cut(s) 607
PspN4I GGNNCC 4 cut(s) 145, 355, 867, 1609
PspPI GGNCC 4 cut(s) 31, 1094, 1193, 1392
PsrI GAACNNNNNNTAC 2 cut(s) 99, 131
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 3 cut(s) 24, 355, 1609
RsaNI GTAC 3 cut(s) 23, 354, 1608
RseI CAYNNNNRTG 2 cut(s) 321, 1647
SaqAI TTAA 8 cut(s) 41, 582, 788, 851, 920, 957, 1038, 1683
SatI GCNGC 2 cut(s) 377, 750
Sau3AI GATC 5 cut(s) 878, 1072, 1279, 1407, 1770
Sau96I GGNCC 4 cut(s) 31, 1094, 1193, 1392
SchI GAGTC 6 cut(s) 222, 335, 767, 1006, 1295, 1313
SduI GDGCHC 2 cut(s) 507, 870
SfaNI GCATC 3 cut(s) 169, 1588, 1603
SfcI CTRYAG 1 cut(s) 78
SfuI TTCGAA 2 cut(s) 620, 677
SinI GGWCC 2 cut(s) 1094, 1193
SmiMI CAYNNNNRTG 2 cut(s) 321, 1647
SmlI CTYRAG 3 cut(s) 64, 581, 956
SmoI CTYRAG 3 cut(s) 64, 581, 956
SphI GCATGC 2 cut(s) 1156, 1255
SseBI AGGCCT 1 cut(s) 1745
StuI AGGCCT 1 cut(s) 1745
StyI CCWWGG 1 cut(s) 1493
TaaI ACNGT 7 cut(s) 269, 514, 695, 721, 1337, 1349, 1768
TaiI ACGT 2 cut(s) 473, 1692
TaqI TCGA 4 cut(s) 620, 677, 999, 1479
TfiI GAWTC 4 cut(s) 8, 479, 961, 1067
Tru1I TTAA 8 cut(s) 41, 582, 788, 851, 920, 957, 1038, 1683
Tru9I TTAA 8 cut(s) 41, 582, 788, 851, 920, 957, 1038, 1683
TscAI CASTG 5 cut(s) 25, 145, 274, 1354, 1773
TseFI GTSAC 3 cut(s) 610, 721, 844
TseI GCWGC 2 cut(s) 376, 749
Tsp45I GTSAC 3 cut(s) 610, 721, 844
TspDTI ATGAA 3 cut(s) 519, 1098, 1197
TspGWI ACGGA 3 cut(s) 1188, 1287, 1319
TspRI CASTG 5 cut(s) 25, 145, 274, 1354, 1773
Vha464I CTTAAG 2 cut(s) 581, 956
VpaK11BI GGWCC 2 cut(s) 1094, 1193
XagI CCTNNNNNAGG 2 cut(s) 348, 1373
XapI RAATTY 4 cut(s) 73, 759, 1505, 1801
XceI RCATGY 4 cut(s) 611, 1156, 1255, 1474
XmiI GTMKAC 1 cut(s) 599
XmnI GAANNNNTTC 1 cut(s) 114
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.