MD09G1061400.v1.1

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005380
Physical Location & Seq
Forward (+)
4195131 .. 4198835
3705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1061400.v1.1.491

Sequence Viewer

Length: 1785 bp
ATGGATGATTTGAGTATCAATTTACCGGTGACCGGTGAAACAACTAGACCAGAGGAGCGTAATCCAAGAAGGAATTCTACAGGGAAAATATTCTCGTTAAACACTGGAGAAAAGATTCCTCATTATCTCAGAGCTTCGACTGGTTCCTGTCATGACAACTGTAAATATGGGAGGAAACATGAAGAGGAAGTGAAGGCAAAATGTCCTATAAAGTTTGTACCAAGAAGGCTTCCAACAAAATCACCTAGCAACCAAAGTCCTGAAGAGAGTATGGTTATTCCTGAGAGGAAGAATGTGTCGGTGGTCAAGATCAAACCCTCGCCTGATTTGAATACTCTTTTGCCTGATACCTGTAACATTACCAAGCAGCGAGTGTCAAAAAAATCCATTGACAGCCAAAGTTCTGTTGGGAGTGAGCCTCTGGCTGAGAGTAAGAAGACATCATCAGCCAAGCCCAAATTGCCATCCTGTTTGAAACCCCATGTCAACGCTGCATCTAAAACCATGAAGCAGGAAGTTTCATCCTCTCTTGATTCCTGTTCAAAACCCATTGTCTCTGCTGTGCCTAAAACCATGAAGCAGGCAGTTTCACCATCTCCTGATAAACTGGAAGTCTCTTCAAAGAAGGGTTCAATAGGACCTAAGGAAAAGAACTTGTTTGTAAAGCATGTCACTTCTTCGAAGCCCAAATCTTTGGCAGTGAAGGAGTTTCCATCTCCTGATATTTCAGGAGGTTTAACTGCTATGAGAAACCGTGATTCATTGATAGTCCGAAGGACTGGGACCTCTTTCAAGATAAGATCTTCAGCTTTGAAGCAAGTGTCTTCTCCTGAATCTGCAGGAGGTTTTAAAGGCAAATCTAACAGTGATGGCAAGATAGGCAAGAGGGCTGGAGCTTCTGTAGTGACTTTGAAGAAAGTATTGCCGTCCCCAAGAGCATCACTGTCCCCCAAACCTTCTCTCAGGAGTGTTGCAATCTTAAAAGCACAGAAGAACAGGCCCGTGAACGTTGTGTCTCCTCTTAAGAACCAGAACAAGATCAGGAAAGCTCAACCTAAGCAAGTCAATGACAAAGAGGTACAGGAGAAGACCTTGTATGTCATCAAGGTTGAAACTGAGAACAACAAACCTCTGGAATCTGATCAGAACAAAAATTGTGAAGTTGAACCATCACCACCTTCAGTGTTGTCGACATCACCCAAGTCGGTATCTGTCCAAAACTCTGAGCCCTTTTCATCCCTTGAAGGCGAAGATGAAGGGTCAGGATATACAATGAGTGAAACAGAAGCTAGCTCTTTCTCAGAAGACAATGAAGCTGACTATATTGACAATTCAGAGATCCTAGGCAAGGATGGCAAAGGGAAACCCAGAAAGTCAGGGATGGTTTGCTCTGAAGACAAGGATGGCGAAACTTTAAAATTCAGGAGGGGAAAGATTGTCGACATACAATTTGAGAGTAATAATCCAAGGAGGCTCAAATTTAGGCGAGGAATAGTGTTGGGGGAGAACCAGAGTGTCAAAGCTGGTTCCTTCAGGCGAAGCTTTAAGAAGAGAGGTGTCGATGCTGATACAATCAGTACCGAACCTGGTGCAGAAAAGGTTGTTTTGAGACATCAAGATGTTCAGGGAAAGAAAGATGAGAAGGGTTTGTTTAACAACGTGATCGAGGAGACAGCAAGTAAACTCGCTGAAACCAGGAAGAGTAAGGTCAAGGCCTTGGTTGGGGCTTTCGAAACAGTCATCTCACTACAAGAGTGCAAGCCTTCTGCAAATGCTGTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

595

Amino Acids

64.73

Weight (kDa)

9.72

Isoelectric Point (pI)

53.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 472 - 582 5.6e-36 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1190, 1440
AclI AACGTT 1 cut(s) 1008
AclWI GGATC 1 cut(s) 1333
AcsI RAATTY 3 cut(s) 73, 1418, 1478
AcuI CTGAAG 5 cut(s) 282, 789, 1164, 1413, 1516
AfaI GTAC 3 cut(s) 219, 1080, 1579
AfiI CCNNNNNNNGG 3 cut(s) 32, 1348, 1722
AflII CTTAAG 1 cut(s) 1022
AgeI ACCGGT 2 cut(s) 25, 32
AjnI CCWGG 2 cut(s) 1585, 1694
AjuI GAANNNNNNNTTGG 2 cut(s) 58, 90
AluBI AGCT 9 cut(s) 134, 809, 896, 1049, 1289, 1293, 1316, 1523, 1542
AluI AGCT 9 cut(s) 134, 809, 896, 1049, 1289, 1293, 1316, 1523, 1542
Alw26I GTCTC 5 cut(s) 559, 619, 1020, 1603, 1664
AlwI GGATC 1 cut(s) 1333
AoxI GGCC 2 cut(s) 998, 1713
ApeKI GCWGC 2 cut(s) 367, 491
ApoI RAATTY 3 cut(s) 73, 1418, 1478
ArsI GACNNNNNNTTYG 2 cut(s) 1433, 1465
AsiGI ACCGGT 2 cut(s) 25, 32
Asp700I GAANNNNTTC 3 cut(s) 73, 89, 114
AspA2I CCTAGG 1 cut(s) 1342
AspS9I GGNCC 3 cut(s) 638, 783, 999
AsuHPI GGTGA 6 cut(s) 40, 47, 234, 582, 1164, 1188
AsuII TTCGAA 2 cut(s) 680, 1731
AsuNHI GCTAGC 1 cut(s) 1289
AvaII GGWCC 2 cut(s) 638, 783
AvrII CCTAGG 1 cut(s) 1342
AxyI CCTNAGG 1 cut(s) 642
BanII GRGCYC 1 cut(s) 1230
BarI GAAGNNNNNNTAC 2 cut(s) 61, 93
BbsI GAAGAC 5 cut(s) 443, 816, 1094, 1311, 1401
BbvI GCAGC 2 cut(s) 379, 478
BccI CCATC 8 cut(s) 472, 601, 721, 863, 1177, 1346, 1375, 1397
BceAI ACGGC 1 cut(s) 910
BcgI CGANNNNNNTGC 2 cut(s) 1571, 1605
BciT130I CCWGG 2 cut(s) 1587, 1696
BclI TGATCA 1 cut(s) 1141
BcoDI GTCTC 5 cut(s) 559, 619, 1020, 1603, 1664
BfaI CTAG 4 cut(s) 45, 246, 1290, 1343
BfmI CTRYAG 3 cut(s) 78, 837, 900
BfrI CTTAAG 1 cut(s) 1022
BglII AGATCT 1 cut(s) 800
BisI GCNGC 2 cut(s) 368, 492
BlnI CCTAGG 1 cut(s) 1342
BlsI GCNGC 2 cut(s) 369, 493
Bme1390I CCNGG 2 cut(s) 1587, 1696
Bme18I GGWCC 2 cut(s) 638, 783
BmgT120I GGNCC 3 cut(s) 638, 783, 999
BmiI GGNNCC 3 cut(s) 145, 784, 1528
BmrFI CCNGG 2 cut(s) 1587, 1696
BmrI ACTGGG 1 cut(s) 789
BmsI GCATC 3 cut(s) 503, 947, 1552
BmtI GCTAGC 1 cut(s) 1293
BmuI ACTGGG 1 cut(s) 789
BpiI GAAGAC 5 cut(s) 443, 816, 1094, 1311, 1401
BplI GAGNNNNNCTC 2 cut(s) 403, 435
BpmI CTGGAG 2 cut(s) 126, 912
Bpu10I CCTNAGC 1 cut(s) 1056
Bpu14I TTCGAA 2 cut(s) 680, 1731
BsaJI CCNNGG 3 cut(s) 1342, 1466, 1716
BsaWI WCCGGW 2 cut(s) 25, 32
BsaXI ACNNNNNCTCC 2 cut(s) 885, 915
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 1348, 1722
Bse118I RCCGGY 2 cut(s) 25, 32
Bse1I ACTGG 4 cut(s) 109, 145, 612, 784
Bse21I CCTNAGG 1 cut(s) 642
BseBI CCWGG 2 cut(s) 1587, 1696
BseDI CCNNGG 3 cut(s) 1342, 1466, 1716
BseGI GGATG 7 cut(s) 10, 464, 521, 1235, 1357, 1386, 1408
BseLI CCNNNNNNNGG 3 cut(s) 32, 1348, 1722
BseMII CTCAG 7 cut(s) 142, 273, 417, 976, 1107, 1215, 1314
BseNI ACTGG 4 cut(s) 109, 145, 612, 784
BseRI GAGGAG 3 cut(s) 68, 1008, 1682
BseXI GCAGC 2 cut(s) 379, 478
BsgI GTGCAG 1 cut(s) 1611
BshFI GGCC 2 cut(s) 1000, 1715
BshTI ACCGGT 2 cut(s) 25, 32
BsiSI CCGG 2 cut(s) 26, 33
BslFI GGGAC 3 cut(s) 796, 913, 931
BslI CCNNNNNNNGG 3 cut(s) 32, 1348, 1722
BsmAI GTCTC 5 cut(s) 559, 619, 1020, 1603, 1664
BsmFI GGGAC 3 cut(s) 796, 913, 931
BsnI GGCC 2 cut(s) 1000, 1715
Bsp119I TTCGAA 2 cut(s) 680, 1731
Bsp1286I GDGCHC 1 cut(s) 1230
Bsp143I GATC 6 cut(s) 309, 800, 1038, 1141, 1338, 1662
BspANI GGCC 2 cut(s) 1000, 1715
BspCNI CTCAG 7 cut(s) 141, 274, 418, 975, 1108, 1216, 1313
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 3 cut(s) 145, 784, 1528
BspMAI CTGCAG 1 cut(s) 841
BspOI GCTAGC 1 cut(s) 1293
BspPI GGATC 1 cut(s) 1333
BspT104I TTCGAA 2 cut(s) 680, 1731
BspTI CTTAAG 1 cut(s) 1022
BsrFI RCCGGY 2 cut(s) 25, 32
BsrI ACTGG 4 cut(s) 109, 145, 612, 784
BssAI RCCGGY 2 cut(s) 25, 32
BssECI CCNNGG 3 cut(s) 1342, 1466, 1716
BssMI GATC 6 cut(s) 309, 800, 1038, 1141, 1338, 1662
BssT1I CCWWGG 3 cut(s) 1342, 1466, 1716
Bst2UI CCWGG 2 cut(s) 1587, 1696
Bst4CI ACNGT 5 cut(s) 161, 755, 866, 945, 1738
Bst6I CTCTTC 5 cut(s) 177, 258, 622, 1544, 1694
BstAFI CTTAAG 1 cut(s) 1022
BstBI TTCGAA 2 cut(s) 680, 1731
BstC8I GCNNGC 3 cut(s) 582, 1291, 1760
BstDEI CTNAG 9 cut(s) 128, 282, 426, 642, 962, 1056, 1116, 1224, 1300
BstEII GGTNACC 1 cut(s) 28
BstENI CCTNNNNNAGG 1 cut(s) 1346
BstF5I GGATG 7 cut(s) 10, 464, 521, 1235, 1357, 1386, 1408
BstKTI GATC 6 cut(s) 312, 803, 1041, 1144, 1341, 1665
BstMAI GTCTC 5 cut(s) 559, 619, 1020, 1603, 1664
BstMBI GATC 6 cut(s) 309, 800, 1038, 1141, 1338, 1662
BstMWI GCNNNNNNNGC 3 cut(s) 460, 879, 1353
BstNI CCWGG 2 cut(s) 1587, 1696
BstNSI RCATGY 1 cut(s) 671
BstPI GGTNACC 1 cut(s) 28
BstSCI CCNGG 2 cut(s) 1585, 1694
BstSFI CTRYAG 3 cut(s) 78, 837, 900
BstV1I GCAGC 2 cut(s) 379, 478
BstV2I GAAGAC 5 cut(s) 443, 816, 1094, 1311, 1401
BstX2I RGATCY 2 cut(s) 800, 1338
BstXI CCANNNNNNTGG 1 cut(s) 694
BstYI RGATCY 2 cut(s) 800, 1338
Bsu36I CCTNAGG 1 cut(s) 642
BsuRI GGCC 2 cut(s) 1000, 1715
BtsCI GGATG 7 cut(s) 10, 464, 521, 1235, 1357, 1386, 1408
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 5 cut(s) 102, 705, 871, 941, 1188
Cac8I GCNNGC 3 cut(s) 582, 1291, 1760
CciI TCATGA 1 cut(s) 151
Cfr10I RCCGGY 2 cut(s) 25, 32
Cfr13I GGNCC 3 cut(s) 638, 783, 999
CsiI ACCWGGT 1 cut(s) 1585
Csp6I GTAC 3 cut(s) 218, 1079, 1578
CspAI ACCGGT 2 cut(s) 25, 32
CviAII CATG 6 cut(s) 152, 179, 482, 505, 574, 668
CviQI GTAC 3 cut(s) 218, 1079, 1578
DdeI CTNAG 9 cut(s) 128, 282, 426, 642, 962, 1056, 1116, 1224, 1300
DpnI GATC 6 cut(s) 311, 802, 1040, 1143, 1340, 1664
DpnII GATC 6 cut(s) 309, 800, 1038, 1141, 1338, 1662
DraI TTTAAA 2 cut(s) 850, 1416
Eam1104I CTCTTC 5 cut(s) 177, 258, 622, 1544, 1694
EarI CTCTTC 5 cut(s) 177, 258, 622, 1544, 1694
Eco130I CCWWGG 3 cut(s) 1342, 1466, 1716
Eco147I AGGCCT 1 cut(s) 1715
Eco24I GRGCYC 1 cut(s) 1230
Eco47I GGWCC 2 cut(s) 638, 783
Eco57I CTGAAG 5 cut(s) 282, 789, 1164, 1413, 1516
Eco81I CCTNAGG 1 cut(s) 642
Eco91I GGTNACC 1 cut(s) 28
EcoNI CCTNNNNNAGG 1 cut(s) 1346
EcoO109I RGGNCCY 2 cut(s) 638, 783
EcoO65I GGTNACC 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 1585, 1694
EcoT14I CCWWGG 3 cut(s) 1342, 1466, 1716
EcoT38I GRGCYC 1 cut(s) 1230
ErhI CCWWGG 3 cut(s) 1342, 1466, 1716
FaeI CATG 6 cut(s) 155, 182, 485, 508, 577, 671
FaqI GGGAC 3 cut(s) 796, 913, 931
FatI CATG 6 cut(s) 151, 178, 481, 504, 573, 667
FbaI TGATCA 1 cut(s) 1141
FblI GTMKAC 2 cut(s) 1190, 1440
Fnu4HI GCNGC 2 cut(s) 368, 492
FokI GGATG 7 cut(s) 17, 451, 508, 1222, 1364, 1393, 1415
FriOI GRGCYC 1 cut(s) 1230
Fsp4HI GCNGC 2 cut(s) 368, 492
FspBI CTAG 4 cut(s) 45, 246, 1290, 1343
GluI GCNGC 2 cut(s) 368, 492
GsuI CTGGAG 2 cut(s) 126, 912
HaeIII GGCC 2 cut(s) 1000, 1715
HapII CCGG 2 cut(s) 26, 33
Hin1II CATG 6 cut(s) 155, 182, 485, 508, 577, 671
HincII GTYRAC 3 cut(s) 487, 1191, 1441
HindII GTYRAC 3 cut(s) 487, 1191, 1441
HindIII AAGCTT 1 cut(s) 1540
HinfI GANTC 5 cut(s) 115, 533, 758, 833, 1136
HpaII CCGG 2 cut(s) 26, 33
HphI GGTGA 6 cut(s) 40, 47, 234, 582, 1164, 1188
Hpy166II GTNNAC 5 cut(s) 487, 1006, 1191, 1441, 1682
Hpy188I TCNGA 8 cut(s) 131, 773, 1141, 1146, 1225, 1303, 1336, 1393
Hpy8I GTNNAC 5 cut(s) 487, 1006, 1191, 1441, 1682
HpyCH4III ACNGT 5 cut(s) 161, 755, 866, 945, 1738
HpyCH4IV ACGT 2 cut(s) 1008, 1659
HpyCH4V TGCA 6 cut(s) 494, 839, 974, 1592, 1758, 1769
HpyF10VI GCNNNNNNNGC 3 cut(s) 460, 879, 1353
HpyF3I CTNAG 9 cut(s) 128, 282, 426, 642, 962, 1056, 1116, 1224, 1300
HpySE526I ACGT 2 cut(s) 1008, 1659
Hsp92II CATG 6 cut(s) 155, 182, 485, 508, 577, 671
Ksp22I TGATCA 1 cut(s) 1141
Kzo9I GATC 6 cut(s) 309, 800, 1038, 1141, 1338, 1662
LmnI GCTCC 2 cut(s) 55, 893
Lsp1109I GCAGC 2 cut(s) 379, 478
LweI GCATC 3 cut(s) 503, 947, 1552
MabI ACCWGGT 1 cut(s) 1585
MaeI CTAG 4 cut(s) 45, 246, 1290, 1343
MaeII ACGT 2 cut(s) 1008, 1659
MaeIII GTNAC 4 cut(s) 28, 353, 670, 904
MalI GATC 6 cut(s) 311, 802, 1040, 1143, 1340, 1664
MboI GATC 6 cut(s) 309, 800, 1038, 1141, 1338, 1662
MflI RGATCY 2 cut(s) 800, 1338
MhlI GDGCHC 1 cut(s) 1230
MluCI AATT 8 cut(s) 19, 73, 458, 1153, 1330, 1418, 1448, 1478
MmeI TCCRAC 1 cut(s) 257
MroXI GAANNNNTTC 3 cut(s) 73, 89, 114
MseI TTAA 8 cut(s) 98, 737, 849, 980, 1023, 1415, 1545, 1653
MslI CAYNNNNRTG 1 cut(s) 1617
MspCI CTTAAG 1 cut(s) 1022
MspI CCGG 2 cut(s) 26, 33
MspR9I CCNGG 2 cut(s) 1587, 1696
MvaI CCWGG 2 cut(s) 1587, 1696
MwoI GCNNNNNNNGC 3 cut(s) 460, 879, 1353
NdeII GATC 6 cut(s) 309, 800, 1038, 1141, 1338, 1662
NheI GCTAGC 1 cut(s) 1289
NlaIII CATG 6 cut(s) 155, 182, 485, 508, 577, 671
NlaIV GGNNCC 3 cut(s) 145, 784, 1528
NmuCI GTSAC 3 cut(s) 28, 670, 904
NspI RCATGY 1 cut(s) 671
NspV TTCGAA 2 cut(s) 680, 1731
PagI TCATGA 1 cut(s) 151
PceI AGGCCT 1 cut(s) 1715
PdmI GAANNNNTTC 3 cut(s) 73, 89, 114
PfeI GAWTC 5 cut(s) 115, 533, 758, 833, 1136
PinAI ACCGGT 2 cut(s) 25, 32
PkrI GCNGC 2 cut(s) 369, 493
PpuMI RGGWCCY 2 cut(s) 638, 783
Psp1406I AACGTT 1 cut(s) 1008
Psp5II RGGWCCY 2 cut(s) 638, 783
Psp6I CCWGG 2 cut(s) 1585, 1694
PspEI GGTNACC 1 cut(s) 28
PspGI CCWGG 2 cut(s) 1585, 1694
PspN4I GGNNCC 3 cut(s) 145, 784, 1528
PspPI GGNCC 3 cut(s) 638, 783, 999
PspPPI RGGWCCY 2 cut(s) 638, 783
PstI CTGCAG 1 cut(s) 841
PsuI RGATCY 2 cut(s) 800, 1338
RsaI GTAC 3 cut(s) 219, 1080, 1579
RsaNI GTAC 3 cut(s) 218, 1079, 1578
RseI CAYNNNNRTG 1 cut(s) 1617
SalI GTCGAC 2 cut(s) 1189, 1439
SaqAI TTAA 8 cut(s) 98, 737, 849, 980, 1023, 1415, 1545, 1653
SatI GCNGC 2 cut(s) 368, 492
Sau3AI GATC 6 cut(s) 309, 800, 1038, 1141, 1338, 1662
Sau96I GGNCC 3 cut(s) 638, 783, 999
ScrFI CCNGG 2 cut(s) 1587, 1696
SduI GDGCHC 1 cut(s) 1230
SexAI ACCWGGT 1 cut(s) 1585
SfaNI GCATC 3 cut(s) 503, 947, 1552
SfcI CTRYAG 3 cut(s) 78, 837, 900
SfuI TTCGAA 2 cut(s) 680, 1731
SinI GGWCC 2 cut(s) 638, 783
SmiMI CAYNNNNRTG 1 cut(s) 1617
SmlI CTYRAG 1 cut(s) 1022
SmoI CTYRAG 1 cut(s) 1022
Sse9I AATT 8 cut(s) 19, 73, 458, 1153, 1330, 1418, 1448, 1478
SseBI AGGCCT 1 cut(s) 1715
SspI AATATT 1 cut(s) 90
SspMI CTAG 4 cut(s) 45, 246, 1290, 1343
StuI AGGCCT 1 cut(s) 1715
StyD4I CCNGG 2 cut(s) 1585, 1694
StyI CCWWGG 3 cut(s) 1342, 1466, 1716
TaaI ACNGT 5 cut(s) 161, 755, 866, 945, 1738
TaiI ACGT 2 cut(s) 1011, 1662
TaqI TCGA 7 cut(s) 137, 680, 1190, 1440, 1560, 1665, 1731
TasI AATT 8 cut(s) 19, 73, 458, 1153, 1330, 1418, 1448, 1478
TfiI GAWTC 5 cut(s) 115, 533, 758, 833, 1136
Tru1I TTAA 8 cut(s) 98, 737, 849, 980, 1023, 1415, 1545, 1653
Tru9I TTAA 8 cut(s) 98, 737, 849, 980, 1023, 1415, 1545, 1653
TscAI CASTG 5 cut(s) 109, 705, 871, 948, 1188
TseFI GTSAC 3 cut(s) 28, 670, 904
TseI GCWGC 2 cut(s) 367, 491
Tsp45I GTSAC 3 cut(s) 28, 670, 904
TspDTI ATGAA 8 cut(s) 195, 510, 521, 590, 750, 1224, 1269, 1326
TspRI CASTG 5 cut(s) 109, 705, 871, 948, 1188
Vha464I CTTAAG 1 cut(s) 1022
VpaK11BI GGWCC 2 cut(s) 638, 783
XagI CCTNNNNNAGG 1 cut(s) 1346
XapI RAATTY 3 cut(s) 73, 1418, 1478
XceI RCATGY 1 cut(s) 671
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XmaJI CCTAGG 1 cut(s) 1342
XmiI GTMKAC 2 cut(s) 1190, 1440
XmnI GAANNNNTTC 3 cut(s) 73, 89, 114
XspI CTAG 4 cut(s) 45, 246, 1290, 1343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.