pycom17g05570

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005380
Physical Location & Seq
Forward (+)
3969407 .. 3970615
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g05570.1

Sequence Viewer

Length: 1209 bp
ATGAAGCAGGAAGTTTTGCCATCTCCTGATAAGCTGGAAGTCTCTTCAAAGAATGGTTCGATAGGACCTAAAGAAAAGAACTTGTCTACAAAGCATGTCACTTCTGCGAAGCCCAAATCTTTGGCAGTGAAAGGGTTACCCTCTCCTGATACCTCAGGAGGTTTAACTGCTCCAAGAAACCGTAATTCAACGATAGGCCAAAGGACAGGGACCTCTTTCAAGATAAAATCTTCAGCTTTGAAGCAAGTGCCTTCTCCTGAATCTGCAGGGGGTTTAAAAGGCAAATCAAACAGTGATGGCAAGATAGGCAAAAGGGCCGGAGCATCCTTAGTGACTTTGAAAAGAGTATTGTCGTCTCCAAGAGCATCATTGTCCCCCAAACCTTCTCTCAGGAGAGTTGCAAGCTTAAAAGCACGGAAGAACAGGCCCATGAACGTTGCGTCTCCTCTTAAGAACCAGAACAAGACCAGAAAAGCTCAACCTAAGCAAGTCAACAGTGATGAGGTACAAGAGAAGACCTTGTATGTCATTAAGGTTGAAACTGAGAATAGCAAACCTCTGGAATCTGATCAAAACAGAAATTGTGAAGTTAAACCATCACAGCGTTCAGCGTCGTCAACACCCAAGTCTGTATCTGTCCAAAACTCTGTGTCCTTTTCATCCCATGAAGGAGAAGATCAAGGGTCTGGATATACAATGAGCGAAACAGAGGTTAGCTCTTTTTCAGAAGACAATGAAGTCGACTATATTGACAATGCAGAGATCCTGGGCGAGGATGGCAAAGGGAAACCCAGAATATCCGGGATGGTTTGTTCTGAGGACAAGGATGGCGAAACTTTAAAATTCAGGAGGGGAAAGGTTGTCGACGTAAAATTTGAGAATTCCAATCCAAGGAGGCTCAAATTCAGGCGAGGAATAGTGTTGGGAGATAACCAAAATGTCAAAGTTGTTTCCCTAAGGCGAAGCTTTAAGAAGAGAGGCGTCGGTGCTGATATAGTTGGTACCGAACCTGGTGCAGAACAGGTTGTTTTGAGACATCAAGATGTTCAGGGAAAGAGAGATGAAAAAGGGTTGTTCAACAACGTGATTGAAGAGACAGCAAGTAAACTTGCTGAAACCAGGAAGAGTAAGGTCAAGGCCTTGGTTGGTGCTTTTGAAACAGTGATCTCACTCCAAGAGCACAAGCCTTCTGCAAACGCTGTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

403

Amino Acids

43.51

Weight (kDa)

9.95

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 280 - 390 1.5e-34 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 737
Acc65I GGTACC 1 cut(s) 1001
AccB1I GGYRCC 1 cut(s) 1001
AccI GTMKAC 3 cut(s) 86, 741, 864
AclI AACGTT 1 cut(s) 435
AclWI GGATC 1 cut(s) 757
AcsI RAATTY 4 cut(s) 842, 872, 880, 902
AcuI CTGAAG 1 cut(s) 216
AcyI GRCGYC 1 cut(s) 981
AfaI GTAC 2 cut(s) 507, 1003
AfiI CCNNNNNNNGG 2 cut(s) 772, 891
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 9 cut(s) 48, 189, 220, 241, 340, 539, 1078, 1091, 1157
AjnI CCWGG 3 cut(s) 765, 1009, 1118
AloI GAACNNNNNNTCC 2 cut(s) 796, 828
AluBI AGCT 6 cut(s) 34, 236, 405, 476, 717, 966
AluI AGCT 6 cut(s) 34, 236, 405, 476, 717, 966
Alw21I GWGCWC 1 cut(s) 1182
Alw26I GTCTC 5 cut(s) 46, 360, 447, 1027, 1088
AlwI GGATC 1 cut(s) 757
AoxI GGCC 4 cut(s) 196, 315, 425, 1137
ApoI RAATTY 4 cut(s) 842, 872, 880, 902
ArsI GACNNNNNNTTYG 2 cut(s) 857, 889
Asp718I GGTACC 1 cut(s) 1001
AspS9I GGNCC 4 cut(s) 65, 210, 315, 426
AsuC2I CCSGG 1 cut(s) 802
AvaII GGWCC 2 cut(s) 65, 210
AxyI CCTNAGG 2 cut(s) 154, 956
BanI GGYRCC 1 cut(s) 1001
BbsI GAAGAC 2 cut(s) 521, 735
Bbv12I GWGCWC 1 cut(s) 1182
BccI CCATC 6 cut(s) 28, 290, 604, 770, 799, 821
BcgI CGANNNNNNTGC 2 cut(s) 995, 1029
BciT130I CCWGG 3 cut(s) 767, 1011, 1120
BclI TGATCA 1 cut(s) 568
BcnI CCSGG 1 cut(s) 802
BcoDI GTCTC 5 cut(s) 46, 360, 447, 1027, 1088
BfmI CTRYAG 1 cut(s) 264
BfrI CTTAAG 1 cut(s) 449
Bme1390I CCNGG 4 cut(s) 767, 802, 1011, 1120
Bme18I GGWCC 2 cut(s) 65, 210
BmgT120I GGNCC 4 cut(s) 65, 210, 315, 426
BmiI GGNNCC 2 cut(s) 211, 1003
BmrFI CCNGG 4 cut(s) 767, 802, 1011, 1120
BmsI GCATC 2 cut(s) 332, 374
BpiI GAAGAC 2 cut(s) 521, 735
BplI GAGNNNNNCTC 2 cut(s) 701, 733
Bpu10I CCTNAGC 1 cut(s) 483
BpuMI CCSGG 1 cut(s) 802
BsaHI GRCGYC 1 cut(s) 981
BsaJI CCNNGG 3 cut(s) 766, 890, 1140
Bsc4I CCNNNNNNNGG 2 cut(s) 772, 891
Bse21I CCTNAGG 2 cut(s) 154, 956
BseBI CCWGG 3 cut(s) 767, 1011, 1120
BseDI CCNNGG 3 cut(s) 766, 890, 1140
BseGI GGATG 5 cut(s) 323, 659, 781, 810, 832
BseLI CCNNNNNNNGG 2 cut(s) 772, 891
BseMII CTCAG 4 cut(s) 168, 403, 534, 807
BseRI GAGGAG 1 cut(s) 435
BsgI GTGCAG 1 cut(s) 1035
BshFI GGCC 4 cut(s) 198, 317, 427, 1139
BshNI GGYRCC 1 cut(s) 1001
BsiHKAI GWGCWC 1 cut(s) 1182
BsiSI CCGG 2 cut(s) 318, 801
BslFI GGGAC 2 cut(s) 223, 358
BslI CCNNNNNNNGG 2 cut(s) 772, 891
BsmAI GTCTC 5 cut(s) 46, 360, 447, 1027, 1088
BsmBI CGTCTC 2 cut(s) 360, 447
BsmFI GGGAC 2 cut(s) 223, 358
BsnI GGCC 4 cut(s) 198, 317, 427, 1139
Bsp1286I GDGCHC 1 cut(s) 1182
Bsp143I GATC 4 cut(s) 568, 676, 762, 1164
BspANI GGCC 4 cut(s) 198, 317, 427, 1139
BspCNI CTCAG 4 cut(s) 167, 402, 535, 808
BspLI GGNNCC 2 cut(s) 211, 1003
BspMAI CTGCAG 1 cut(s) 268
BspPI GGATC 1 cut(s) 757
BspT107I GGYRCC 1 cut(s) 1001
BspTI CTTAAG 1 cut(s) 449
BssECI CCNNGG 3 cut(s) 766, 890, 1140
BssMI GATC 4 cut(s) 568, 676, 762, 1164
BssNI GRCGYC 1 cut(s) 981
BssT1I CCWWGG 2 cut(s) 890, 1140
Bst2UI CCWGG 3 cut(s) 767, 1011, 1120
Bst4CI ACNGT 4 cut(s) 182, 293, 497, 1162
Bst6I CTCTTC 4 cut(s) 49, 968, 1086, 1118
BstACI GRCGYC 1 cut(s) 981
BstAFI CTTAAG 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 7 cut(s) 154, 328, 389, 483, 543, 816, 956
BstEII GGTNACC 1 cut(s) 135
BstENI CCTNNNNNAGG 1 cut(s) 770
BstF5I GGATG 5 cut(s) 323, 659, 781, 810, 832
BstKTI GATC 4 cut(s) 571, 679, 765, 1167
BstMAI GTCTC 5 cut(s) 46, 360, 447, 1027, 1088
BstMBI GATC 4 cut(s) 568, 676, 762, 1164
BstMWI GCNNNNNNNGC 2 cut(s) 306, 777
BstNI CCWGG 3 cut(s) 767, 1011, 1120
BstNSI RCATGY 1 cut(s) 98
BstPI GGTNACC 1 cut(s) 135
BstSCI CCNGG 4 cut(s) 765, 800, 1009, 1118
BstSFI CTRYAG 1 cut(s) 264
BstV2I GAAGAC 2 cut(s) 521, 735
BstX2I RGATCY 1 cut(s) 762
BstXI CCANNNNNNTGG 1 cut(s) 121
BstYI RGATCY 1 cut(s) 762
Bsu36I CCTNAGG 2 cut(s) 154, 956
BsuRI GGCC 4 cut(s) 198, 317, 427, 1139
BtsCI GGATG 5 cut(s) 323, 659, 781, 810, 832
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 4 cut(s) 132, 298, 502, 1167
Cac8I GCNNGC 1 cut(s) 403
Cfr13I GGNCC 4 cut(s) 65, 210, 315, 426
CseI GACGC 3 cut(s) 429, 600, 970
CsiI ACCWGGT 1 cut(s) 1009
Csp6I GTAC 2 cut(s) 506, 1002
CviAII CATG 3 cut(s) 95, 430, 665
CviQI GTAC 2 cut(s) 506, 1002
DdeI CTNAG 7 cut(s) 154, 328, 389, 483, 543, 816, 956
DpnI GATC 4 cut(s) 570, 678, 764, 1166
DpnII GATC 4 cut(s) 568, 676, 762, 1164
DraI TTTAAA 2 cut(s) 276, 840
DrdI GACNNNNNNGTC 1 cut(s) 737
DseDI GACNNNNNNGTC 1 cut(s) 737
Eam1104I CTCTTC 4 cut(s) 49, 968, 1086, 1118
EarI CTCTTC 4 cut(s) 49, 968, 1086, 1118
Eco130I CCWWGG 2 cut(s) 890, 1140
Eco147I AGGCCT 1 cut(s) 1139
Eco47I GGWCC 2 cut(s) 65, 210
Eco57I CTGAAG 1 cut(s) 216
Eco81I CCTNAGG 2 cut(s) 154, 956
Eco91I GGTNACC 1 cut(s) 135
EcoNI CCTNNNNNAGG 1 cut(s) 770
EcoO109I RGGNCCY 2 cut(s) 65, 210
EcoO65I GGTNACC 1 cut(s) 135
EcoRI GAATTC 1 cut(s) 880
EcoRII CCWGG 3 cut(s) 765, 1009, 1118
EcoT14I CCWWGG 2 cut(s) 890, 1140
ErhI CCWWGG 2 cut(s) 890, 1140
Esp3I CGTCTC 2 cut(s) 360, 447
FaeI CATG 3 cut(s) 98, 433, 668
FaiI YATR 7 cut(s) 96, 431, 525, 666, 693, 747, 995
FaqI GGGAC 2 cut(s) 223, 358
FatI CATG 3 cut(s) 94, 429, 664
FbaI TGATCA 1 cut(s) 568
FblI GTMKAC 3 cut(s) 86, 741, 864
FokI GGATG 5 cut(s) 310, 646, 788, 817, 839
HaeIII GGCC 4 cut(s) 198, 317, 427, 1139
HapII CCGG 2 cut(s) 318, 801
HgaI GACGC 3 cut(s) 429, 600, 970
Hin1I GRCGYC 1 cut(s) 981
Hin1II CATG 3 cut(s) 98, 433, 668
HincII GTYRAC 4 cut(s) 493, 618, 742, 865
HindII GTYRAC 4 cut(s) 493, 618, 742, 865
HindIII AAGCTT 2 cut(s) 403, 964
HinfI GANTC 2 cut(s) 260, 563
HpaII CCGG 2 cut(s) 318, 801
Hpy166II GTNNAC 6 cut(s) 87, 493, 618, 742, 865, 1106
Hpy188I TCNGA 3 cut(s) 568, 727, 817
Hpy8I GTNNAC 6 cut(s) 87, 493, 618, 742, 865, 1106
Hpy99I CGWCG 3 cut(s) 616, 869, 986
HpyAV CCTTC 4 cut(s) 261, 393, 662, 1197
HpyCH4III ACNGT 4 cut(s) 182, 293, 497, 1162
HpyCH4IV ACGT 3 cut(s) 435, 867, 1083
HpyCH4V TGCA 5 cut(s) 266, 401, 758, 1016, 1193
HpyF10VI GCNNNNNNNGC 2 cut(s) 306, 777
HpyF3I CTNAG 7 cut(s) 154, 328, 389, 483, 543, 816, 956
HpySE526I ACGT 3 cut(s) 435, 867, 1083
Hsp92I GRCGYC 1 cut(s) 981
Hsp92II CATG 3 cut(s) 98, 433, 668
KpnI GGTACC 1 cut(s) 1005
Ksp22I TGATCA 1 cut(s) 568
Kzo9I GATC 4 cut(s) 568, 676, 762, 1164
LmnI GCTCC 2 cut(s) 175, 320
LweI GCATC 2 cut(s) 332, 374
MabI ACCWGGT 1 cut(s) 1009
MaeII ACGT 3 cut(s) 435, 867, 1083
MaeIII GTNAC 3 cut(s) 97, 135, 331
MalI GATC 4 cut(s) 570, 678, 764, 1166
MboI GATC 4 cut(s) 568, 676, 762, 1164
MboII GAAGA 9 cut(s) 36, 222, 430, 526, 686, 740, 985, 1103, 1135
MflI RGATCY 1 cut(s) 762
MhlI GDGCHC 1 cut(s) 1182
MluCI AATT 6 cut(s) 184, 580, 842, 872, 880, 902
MseI TTAA 8 cut(s) 164, 275, 407, 450, 531, 591, 839, 969
MslI CAYNNNNRTG 1 cut(s) 1041
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 2 cut(s) 318, 801
MspR9I CCNGG 4 cut(s) 767, 802, 1011, 1120
MvaI CCWGG 3 cut(s) 767, 1011, 1120
MwoI GCNNNNNNNGC 2 cut(s) 306, 777
NciI CCSGG 1 cut(s) 802
NdeII GATC 4 cut(s) 568, 676, 762, 1164
NlaIII CATG 3 cut(s) 98, 433, 668
NlaIV GGNNCC 2 cut(s) 211, 1003
NmuCI GTSAC 2 cut(s) 97, 331
NspI RCATGY 1 cut(s) 98
PceI AGGCCT 1 cut(s) 1139
PfeI GAWTC 2 cut(s) 260, 563
PfoI TCCNGGA 1 cut(s) 800
PpuMI RGGWCCY 2 cut(s) 65, 210
Psp1406I AACGTT 1 cut(s) 435
Psp5II RGGWCCY 2 cut(s) 65, 210
Psp6I CCWGG 3 cut(s) 765, 1009, 1118
PspEI GGTNACC 1 cut(s) 135
PspGI CCWGG 3 cut(s) 765, 1009, 1118
PspN4I GGNNCC 2 cut(s) 211, 1003
PspPI GGNCC 4 cut(s) 65, 210, 315, 426
PspPPI RGGWCCY 2 cut(s) 65, 210
PstI CTGCAG 1 cut(s) 268
PsuI RGATCY 1 cut(s) 762
RsaI GTAC 2 cut(s) 507, 1003
RsaNI GTAC 2 cut(s) 506, 1002
RseI CAYNNNNRTG 1 cut(s) 1041
SalI GTCGAC 2 cut(s) 740, 863
SaqAI TTAA 8 cut(s) 164, 275, 407, 450, 531, 591, 839, 969
Sau3AI GATC 4 cut(s) 568, 676, 762, 1164
Sau96I GGNCC 4 cut(s) 65, 210, 315, 426
ScrFI CCNGG 4 cut(s) 767, 802, 1011, 1120
SduI GDGCHC 1 cut(s) 1182
SexAI ACCWGGT 1 cut(s) 1009
SfaNI GCATC 2 cut(s) 332, 374
SfcI CTRYAG 1 cut(s) 264
SinI GGWCC 2 cut(s) 65, 210
SmiMI CAYNNNNRTG 1 cut(s) 1041
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 6 cut(s) 184, 580, 842, 872, 880, 902
SseBI AGGCCT 1 cut(s) 1139
StuI AGGCCT 1 cut(s) 1139
StyD4I CCNGG 4 cut(s) 765, 800, 1009, 1118
StyI CCWWGG 2 cut(s) 890, 1140
TaaI ACNGT 4 cut(s) 182, 293, 497, 1162
TaiI ACGT 3 cut(s) 438, 870, 1086
TaqI TCGA 3 cut(s) 59, 741, 864
TasI AATT 6 cut(s) 184, 580, 842, 872, 880, 902
TfiI GAWTC 2 cut(s) 260, 563
Tru1I TTAA 8 cut(s) 164, 275, 407, 450, 531, 591, 839, 969
Tru9I TTAA 8 cut(s) 164, 275, 407, 450, 531, 591, 839, 969
TscAI CASTG 4 cut(s) 132, 298, 502, 1167
TseFI GTSAC 2 cut(s) 97, 331
Tsp45I GTSAC 2 cut(s) 97, 331
TspDTI ATGAA 6 cut(s) 17, 446, 648, 681, 750, 1077
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 4 cut(s) 132, 298, 502, 1167
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 2 cut(s) 65, 210
XagI CCTNNNNNAGG 1 cut(s) 770
XapI RAATTY 4 cut(s) 842, 872, 880, 902
XceI RCATGY 1 cut(s) 98
XmiI GTMKAC 3 cut(s) 86, 741, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.