RLG00000021627

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005380
Physical Location & Seq
Reverse (-)
76848589 .. 76850298
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021627

Sequence Viewer

Length: 1710 bp
ATGGCAAGTATCAGTGTACCAACGGCCCAAGAATTAACAAAGCCAGATGAGGCTAAATCGAGACGAAATTCTACAGGAAAGATATTATCGTCAAACAGTGGAGAAAAAGTTCCTCATTATCTCAGGGCTTCCACGGGTTCCTGTCATGATTTTTGTAAATATGGGAGGAAAGATGCATTCGAAGAGAAGGAAAGATGCCCCATACGGGTTGTACCAAGAAGACTCTCAACTAAATCACTTGATAGCCAAAACACAGCAGAAAGTGTAGTTTTGCCAGAGATCAAGGATATATCAGTGATCAAGCTCAATCATTCACCCGAGTCCAAAACCCTCTCACCTGATACATGTACCATCGCCAAGCAGGCCAAGCAGCAACTGCCAAAAAGGTCAGCTGACAGAAAAACTGCTGTTGGGAGTGAGCTTCTTGCTGAGAGGAAGAAGCCATCATTGGTGAAGTTCAAAACGTCATCCAATTCAAAACCTCCTTTATCTGCTGTGCCCAAAACTGTGAAGCAGGAAGTTTCATCATCTTCTGAAAAGCTGAAAGTCTCTTCAAAGAAAGGTTGGACAAAACTTAAGGAGAAAGACTTGTCTACAAAACATGTCACTTCTTTGAAGCCAAAATCTCTGACCACGAAGGAATTGTCATCTCCTGATACTTCAGGAGGTTCAGAGGGGATAAGAAACAGTAATTCAAAGGTAGGCCAGAGGACAGTCACCTCTCTGAAGCCAAAATCTTTGGCTGTGAAGCAAATTTCTTCTCTTGACTCTTCAGGAGGCTTAAATGGCGATAGAAAAAGTGATGCGAAGATAGGCAAGAGGGCAGGAACGTCCTTTGTTGCTTTAAAGAAAGTATTGGTGCCCCCAACAGGATCATTGTCCCCAAAACCTTCTCTCAGGAGAGTTGCAAGCTTAAAAGCACAGGACAGGAATGTGAAAGTTACTCCTCTTAAGAATCAGAACAAGATCGGAGAAGTTATAGCTAAGCAACTCAACAATGATGAGACTCCGGAGAAGACCTTGTATGTCATTAAGATTGAGACTGAGAATAAACCTCTGGAATCTGATCAAAACAACAATTGTGAAGGTCCACCACCTTCATCCTCCTCATCATCATCGCCCAAGTCTTTGTCCCTCCCAAACAGCATGTCCCTTTCACCCCTTGAAGGAGAAGATCAAGAGTCTGAATATACAATGACAGAAACAGAAGAGGAGTCTTTCTCAGAAGACGATGAAGTTGACAACGAAGAAAATGCAGAGACCCTAGATGAGGATTACAAAGGGAAGCCCAGAAAGTCTGGGATGGTTTGTTCTGAAGATGTGGATGGCCATCCTTTAAAGTTTAGGAGGGGAAAGGTGGTTGACATGCAATTTCAGAATAATAGACCAAGGAGGCTCAAATTTAGGCGAGGAAAAGTGTTGGGAGAGAACGAAAATGTCAAAGCTGATGCCCAAAGGCGAAGATATAAGAAGAGAGAAGGTGTTGATGGCGATGCAATTGGTACCGAACCTGGTGCAGAAAAGGTTGTTTTGAGACATCAAGATGTTCAGGGGAAGAAAGATGAGAAGGGGTTGTTTAATAATGTAATTGAAGAAACTGCAAGTAAACTGGTTGAAACCCGGAAGAGTAAGGTCAAGGCCTTAGTTGGTGCTTTTGAAACAGTGATCTCACTTCAAGAGTGCAAACCTTCTGCAAATTCTGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

570

Amino Acids

62.4

Weight (kDa)

9.5

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 446 - 557 4.7e-36 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1502
AccB1I GGYRCC 2 cut(s) 859, 1502
AccI GTMKAC 1 cut(s) 593
AccIII TCCGGA 1 cut(s) 1009
AclWI GGATC 1 cut(s) 880
AcoI YGGCCR 1 cut(s) 1327
AcsI RAATTY 4 cut(s) 67, 753, 1400, 1696
AcuI CTGAAG 4 cut(s) 645, 746, 756, 1335
AfaI GTAC 4 cut(s) 18, 213, 349, 1504
AfiI CCNNNNNNNGG 4 cut(s) 205, 869, 1166, 1270
AflII CTTAAG 2 cut(s) 575, 950
AflIII ACRYGT 2 cut(s) 344, 601
AjnI CCWGG 1 cut(s) 1510
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AloI GAACNNNNNNTCC 4 cut(s) 93, 125, 1294, 1326
AluBI AGCT 7 cut(s) 304, 392, 421, 541, 912, 983, 1445
AluI AGCT 7 cut(s) 304, 392, 421, 541, 912, 983, 1445
Alw26I GTCTC 6 cut(s) 55, 553, 998, 1034, 1253, 1528
AlwI GGATC 1 cut(s) 880
AlwNI CAGNNNCTG 1 cut(s) 376
Ama87I CYCGRG 1 cut(s) 317
Aor13HI TCCGGA 1 cut(s) 1009
AoxI GGCC 5 cut(s) 24, 363, 703, 1327, 1638
ApeKI GCWGC 1 cut(s) 370
ApoI RAATTY 4 cut(s) 67, 753, 1400, 1696
ArsI GACNNNNNNTTYG 4 cut(s) 629, 661, 1133, 1165
Asp700I GAANNNNTTC 1 cut(s) 108
Asp718I GGTACC 1 cut(s) 1502
AspS9I GGNCC 2 cut(s) 25, 1088
AsuC2I CCSGG 1 cut(s) 1621
AsuHPI GGTGA 5 cut(s) 306, 327, 463, 709, 1149
AsuII TTCGAA 1 cut(s) 180
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 1088
BaeGI GKGCMC 2 cut(s) 501, 864
BalI TGGCCA 1 cut(s) 1329
BanI GGYRCC 2 cut(s) 859, 1502
BbsI GAAGAC 3 cut(s) 226, 1022, 1233
BbvI GCAGC 1 cut(s) 382
BccI CCATC 6 cut(s) 359, 451, 1297, 1319, 1338, 1481
BceAI ACGGC 1 cut(s) 39
BcgI CGANNNNNNTGC 4 cut(s) 1235, 1269, 1496, 1530
BciT130I CCWGG 1 cut(s) 1512
BclI TGATCA 2 cut(s) 297, 1066
BcnI CCSGG 1 cut(s) 1621
BcoDI GTCTC 6 cut(s) 55, 553, 998, 1034, 1253, 1528
BfaI CTAG 1 cut(s) 1265
BfmI CTRYAG 1 cut(s) 72
BfrI CTTAAG 2 cut(s) 575, 950
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 1 cut(s) 371
BlpI GCTNAGC 1 cut(s) 984
BlsI GCNGC 1 cut(s) 372
Bme1390I CCNGG 2 cut(s) 1512, 1621
Bme18I GGWCC 1 cut(s) 1088
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 2 cut(s) 25, 1088
BmiI GGNNCC 3 cut(s) 139, 861, 1504
BmrFI CCNGG 2 cut(s) 1512, 1621
BmsI GCATC 5 cut(s) 163, 185, 793, 1438, 1483
BpiI GAAGAC 3 cut(s) 226, 1022, 1233
BplI GAGNNNNNCTC 2 cut(s) 1205, 1237
Bpu1102I GCTNAGC 1 cut(s) 984
Bpu14I TTCGAA 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 1621
BsaBI GATNNNNATC 1 cut(s) 1329
BsaI GGTCTC 1 cut(s) 1253
BsaJI CCNNGG 2 cut(s) 132, 1388
BsaWI WCCGGW 1 cut(s) 1009
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 869, 1166, 1270
Bse1I ACTGG 1 cut(s) 1614
Bse8I GATNNNNATC 1 cut(s) 1329
BseAI TCCGGA 1 cut(s) 1009
BseBI CCWGG 1 cut(s) 1512
BseDI CCNNGG 2 cut(s) 132, 1388
BseGI GGATG 5 cut(s) 467, 1100, 1308, 1330, 1330
BseJI GATNNNNATC 1 cut(s) 1329
BseLI CCNNNNNNNGG 4 cut(s) 205, 869, 1166, 1270
BseMII CTCAG 5 cut(s) 136, 420, 910, 1035, 1236
BseNI ACTGG 1 cut(s) 1614
BseRI GAGGAG 3 cut(s) 936, 1096, 1226
BseSI GKGCMC 2 cut(s) 501, 864
BseXI GCAGC 1 cut(s) 382
BsgI GTGCAG 1 cut(s) 1536
BshFI GGCC 5 cut(s) 26, 365, 705, 1329, 1640
BshNI GGYRCC 2 cut(s) 859, 1502
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 2 cut(s) 1010, 1621
BslFI GGGAC 3 cut(s) 865, 1117, 1135
BslI CCNNNNNNNGG 4 cut(s) 205, 869, 1166, 1270
BsmAI GTCTC 6 cut(s) 55, 553, 998, 1034, 1253, 1528
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 3 cut(s) 865, 1117, 1135
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 5 cut(s) 26, 365, 705, 1329, 1640
Bso31I GGTCTC 1 cut(s) 1253
BsoBI CYCGRG 1 cut(s) 317
Bsp119I TTCGAA 1 cut(s) 180
Bsp1286I GDGCHC 2 cut(s) 501, 864
Bsp13I TCCGGA 1 cut(s) 1009
Bsp143I GATC 7 cut(s) 279, 297, 872, 966, 1066, 1174, 1665
Bsp1720I GCTNAGC 1 cut(s) 984
BspANI GGCC 5 cut(s) 26, 365, 705, 1329, 1640
BspCNI CTCAG 5 cut(s) 135, 421, 909, 1036, 1235
BspEI TCCGGA 1 cut(s) 1009
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 3 cut(s) 139, 861, 1504
BspPI GGATC 1 cut(s) 880
BspT104I TTCGAA 1 cut(s) 180
BspT107I GGYRCC 2 cut(s) 859, 1502
BspTI CTTAAG 2 cut(s) 575, 950
BspTNI GGTCTC 1 cut(s) 1253
BsrI ACTGG 1 cut(s) 1614
BssECI CCNNGG 2 cut(s) 132, 1388
BssMI GATC 7 cut(s) 279, 297, 872, 966, 1066, 1174, 1665
BssT1I CCWWGG 1 cut(s) 1388
Bst2UI CCWGG 1 cut(s) 1512
Bst4CI ACNGT 5 cut(s) 98, 508, 689, 715, 1663
Bst6I CTCTTC 6 cut(s) 177, 556, 775, 1203, 1466, 1619
BstAFI CTTAAG 2 cut(s) 575, 950
BstAPI GCANNNNNTGC 1 cut(s) 376
BstBI TTCGAA 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 363, 910
BstDEI CTNAG 7 cut(s) 122, 429, 896, 984, 1044, 1222, 1642
BstDSI CCRYGG 1 cut(s) 132
BstENI CCTNNNNNAGG 1 cut(s) 1268
BstF5I GGATG 5 cut(s) 467, 1100, 1308, 1330, 1330
BstKTI GATC 7 cut(s) 282, 300, 875, 969, 1069, 1177, 1668
BstMAI GTCTC 6 cut(s) 55, 553, 998, 1034, 1253, 1528
BstMBI GATC 7 cut(s) 279, 297, 872, 966, 1066, 1174, 1665
BstMWI GCNNNNNNNGC 4 cut(s) 362, 367, 376, 786
BstNI CCWGG 1 cut(s) 1512
BstNSI RCATGY 4 cut(s) 348, 605, 1150, 1369
BstSCI CCNGG 2 cut(s) 1510, 1619
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 2 cut(s) 501, 864
BstV1I GCAGC 1 cut(s) 382
BstV2I GAAGAC 3 cut(s) 226, 1022, 1233
BsuRI GGCC 5 cut(s) 26, 365, 705, 1329, 1640
BtgI CCRYGG 1 cut(s) 132
BtgZI GCGATG 3 cut(s) 337, 1101, 1506
BtsCI GGATG 5 cut(s) 467, 1100, 1308, 1330, 1330
BtsIMutI CAGTG 4 cut(s) 19, 103, 300, 1668
Cac8I GCNNGC 2 cut(s) 363, 910
CaiI CAGNNNCTG 1 cut(s) 376
CciI TCATGA 1 cut(s) 145
Cfr13I GGNCC 2 cut(s) 25, 1088
CsiI ACCWGGT 1 cut(s) 1510
Csp6I GTAC 4 cut(s) 17, 212, 348, 1503
CviAII CATG 5 cut(s) 146, 345, 602, 1147, 1366
CviQI GTAC 4 cut(s) 17, 212, 348, 1503
DdeI CTNAG 7 cut(s) 122, 429, 896, 984, 1044, 1222, 1642
DpnI GATC 7 cut(s) 281, 299, 874, 968, 1068, 1176, 1667
DpnII GATC 7 cut(s) 279, 297, 872, 966, 1066, 1174, 1665
DraI TTTAAA 2 cut(s) 846, 1338
EaeI YGGCCR 1 cut(s) 1327
Eam1104I CTCTTC 6 cut(s) 177, 556, 775, 1203, 1466, 1619
EarI CTCTTC 6 cut(s) 177, 556, 775, 1203, 1466, 1619
Eco130I CCWWGG 1 cut(s) 1388
Eco147I AGGCCT 1 cut(s) 1640
Eco31I GGTCTC 1 cut(s) 1253
Eco47I GGWCC 1 cut(s) 1088
Eco57I CTGAAG 4 cut(s) 645, 746, 756, 1335
Eco88I CYCGRG 1 cut(s) 317
EcoNI CCTNNNNNAGG 1 cut(s) 1268
EcoRII CCWGG 1 cut(s) 1510
EcoT14I CCWWGG 1 cut(s) 1388
EcoT22I ATGCAT 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 1388
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 5 cut(s) 149, 348, 605, 1150, 1369
FaqI GGGAC 3 cut(s) 865, 1117, 1135
FatI CATG 5 cut(s) 145, 344, 601, 1146, 1365
FbaI TGATCA 2 cut(s) 297, 1066
FblI GTMKAC 1 cut(s) 593
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 5 cut(s) 454, 1087, 1315, 1317, 1337
Fsp4HI GCNGC 1 cut(s) 371
FspBI CTAG 1 cut(s) 1265
GluI GCNGC 1 cut(s) 371
HaeIII GGCC 5 cut(s) 26, 365, 705, 1329, 1640
HapII CCGG 2 cut(s) 1010, 1621
Hin1II CATG 5 cut(s) 149, 348, 605, 1150, 1369
HincII GTYRAC 2 cut(s) 1240, 1363
HindII GTYRAC 2 cut(s) 1240, 1363
HindIII AAGCTT 1 cut(s) 910
HinfI GANTC 8 cut(s) 222, 320, 767, 955, 1006, 1061, 1181, 1214
HpaII CCGG 2 cut(s) 1010, 1621
HphI GGTGA 5 cut(s) 306, 327, 463, 709, 1149
Hpy166II GTNNAC 6 cut(s) 17, 594, 1091, 1240, 1363, 1607
Hpy8I GTNNAC 6 cut(s) 17, 594, 1091, 1240, 1363, 1607
HpyAV CCTTC 9 cut(s) 181, 631, 900, 1079, 1107, 1160, 1472, 1561, 1698
HpyCH4III ACNGT 5 cut(s) 98, 508, 689, 715, 1663
HpyCH4IV ACGT 2 cut(s) 464, 830
HpyCH4V TGCA 9 cut(s) 176, 908, 1256, 1369, 1496, 1517, 1601, 1683, 1694
HpyF10VI GCNNNNNNNGC 4 cut(s) 362, 367, 376, 786
HpyF3I CTNAG 7 cut(s) 122, 429, 896, 984, 1044, 1222, 1642
HpySE526I ACGT 2 cut(s) 464, 830
Hsp92II CATG 5 cut(s) 149, 348, 605, 1150, 1369
Kpn2I TCCGGA 1 cut(s) 1009
KpnI GGTACC 1 cut(s) 1506
Ksp22I TGATCA 2 cut(s) 297, 1066
Kzo9I GATC 7 cut(s) 279, 297, 872, 966, 1066, 1174, 1665
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 5 cut(s) 163, 185, 793, 1438, 1483
MabI ACCWGGT 1 cut(s) 1510
MaeI CTAG 1 cut(s) 1265
MaeII ACGT 2 cut(s) 464, 830
MaeIII GTNAC 3 cut(s) 604, 715, 940
MalI GATC 7 cut(s) 281, 299, 874, 968, 1068, 1176, 1667
MboI GATC 7 cut(s) 279, 297, 872, 966, 1066, 1174, 1665
MfeI CAATTG 2 cut(s) 1078, 1497
MhlI GDGCHC 2 cut(s) 501, 864
MlsI TGGCCA 1 cut(s) 1329
MluNI TGGCCA 1 cut(s) 1329
MlyI GAGTC 6 cut(s) 216, 329, 761, 1000, 1190, 1223
MmeI TCCRAC 1 cut(s) 545
Mox20I TGGCCA 1 cut(s) 1329
Mph1103I ATGCAT 1 cut(s) 178
MroI TCCGGA 1 cut(s) 1009
MroXI GAANNNNTTC 1 cut(s) 108
MscI TGGCCA 1 cut(s) 1329
MseI TTAA 9 cut(s) 35, 576, 782, 845, 914, 951, 1032, 1337, 1578
MslI CAYNNNNRTG 1 cut(s) 1542
Msp20I TGGCCA 1 cut(s) 1329
MspA1I CMGCKG 1 cut(s) 392
MspCI CTTAAG 2 cut(s) 575, 950
MspI CCGG 2 cut(s) 1010, 1621
MspR9I CCNGG 2 cut(s) 1512, 1621
MunI CAATTG 2 cut(s) 1078, 1497
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 1512
MwoI GCNNNNNNNGC 4 cut(s) 362, 367, 376, 786
NciI CCSGG 1 cut(s) 1621
NdeII GATC 7 cut(s) 279, 297, 872, 966, 1066, 1174, 1665
NlaIII CATG 5 cut(s) 149, 348, 605, 1150, 1369
NlaIV GGNNCC 3 cut(s) 139, 861, 1504
NmuCI GTSAC 2 cut(s) 604, 715
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 4 cut(s) 348, 605, 1150, 1369
NspV TTCGAA 1 cut(s) 180
PagI TCATGA 1 cut(s) 145
PceI AGGCCT 1 cut(s) 1640
PciI ACATGT 2 cut(s) 344, 601
PctI GAATGC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 2 cut(s) 955, 1061
PkrI GCNGC 1 cut(s) 372
PleI GAGTC 6 cut(s) 216, 328, 761, 1000, 1189, 1222
PpsI GAGTC 6 cut(s) 216, 328, 761, 1000, 1189, 1222
PscI ACATGT 2 cut(s) 344, 601
Psp6I CCWGG 1 cut(s) 1510
PspGI CCWGG 1 cut(s) 1510
PspN4I GGNNCC 3 cut(s) 139, 861, 1504
PspPI GGNCC 2 cut(s) 25, 1088
PstNI CAGNNNCTG 1 cut(s) 376
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 4 cut(s) 18, 213, 349, 1504
RsaNI GTAC 4 cut(s) 17, 212, 348, 1503
RseI CAYNNNNRTG 1 cut(s) 1542
SaqAI TTAA 9 cut(s) 35, 576, 782, 845, 914, 951, 1032, 1337, 1578
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 7 cut(s) 279, 297, 872, 966, 1066, 1174, 1665
Sau96I GGNCC 2 cut(s) 25, 1088
SchI GAGTC 6 cut(s) 216, 329, 761, 1000, 1190, 1223
ScrFI CCNGG 2 cut(s) 1512, 1621
SduI GDGCHC 2 cut(s) 501, 864
SexAI ACCWGGT 1 cut(s) 1510
SfaNI GCATC 5 cut(s) 163, 185, 793, 1438, 1483
SfcI CTRYAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 180
SinI GGWCC 1 cut(s) 1088
SmiMI CAYNNNNRTG 1 cut(s) 1542
SmlI CTYRAG 2 cut(s) 575, 950
SmoI CTYRAG 2 cut(s) 575, 950
SseBI AGGCCT 1 cut(s) 1640
SspMI CTAG 1 cut(s) 1265
StuI AGGCCT 1 cut(s) 1640
StyD4I CCNGG 2 cut(s) 1510, 1619
StyI CCWWGG 1 cut(s) 1388
TaaI ACNGT 5 cut(s) 98, 508, 689, 715, 1663
TaiI ACGT 2 cut(s) 467, 833
TaqI TCGA 2 cut(s) 59, 180
TfiI GAWTC 2 cut(s) 955, 1061
Tru1I TTAA 9 cut(s) 35, 576, 782, 845, 914, 951, 1032, 1337, 1578
Tru9I TTAA 9 cut(s) 35, 576, 782, 845, 914, 951, 1032, 1337, 1578
TscAI CASTG 4 cut(s) 19, 103, 300, 1668
TseFI GTSAC 2 cut(s) 604, 715
TseI GCWGC 1 cut(s) 370
Tsp45I GTSAC 2 cut(s) 604, 715
TspDTI ATGAA 3 cut(s) 513, 1089, 1248
TspRI CASTG 4 cut(s) 19, 103, 300, 1668
Vha464I CTTAAG 2 cut(s) 575, 950
VpaK11BI GGWCC 1 cut(s) 1088
XagI CCTNNNNNAGG 1 cut(s) 1268
XapI RAATTY 4 cut(s) 67, 753, 1400, 1696
XceI RCATGY 4 cut(s) 348, 605, 1150, 1369
XmiI GTMKAC 1 cut(s) 593
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 1 cut(s) 1265
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.