Rh2BG602000

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005380
Physical Location & Seq
Reverse (-)
82203248 .. 82206585
3338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG602000.1

Sequence Viewer

Length: 1686 bp
ATGGCAAGTATCAGTGTACCAACAGATGAGGCTAAATCGAGACGAAATTCTACAGGAAAGATATTATCGTCAATCAGTGGAGAAAAAGTTCCTCATTATCTCAGGGCTTCCACTGGTTCCTGTCATGATTTTTGTAAATATGGGAGGAAAGATGCATTTGAAGAGAAGGAAAGATGCCCCATACGGGTTGTACCAAGAAGACTCTCAACCAAATCACTTGGTAGCCAAAACTCAGCAGAAAGTGTAGTTTTGCCAGAGATCAAGAATATATCAGTGATCAAGCTCAATCATTCACCCGAGTCCAAAATCCTCTCACCTGATACATGTACCATCGCCAAGCAGGCTAAGCAGCAACTGCCAAAAAGGTCAGCTGACAGAAAAACTGCTGTTGGGAGTGAGCTTCTTGCTGAGAGGAAGAAGCCATCATTGGTGAAGTTCAAAACGTCATCCAATGCAAAACCTCCTGTATCTGCTGTGCCCAAAACTGTGAAGCAGGAAGTTTCATCATCTCCTGAAAAGCGGAAATTCTCTTCAAAGAAAGGTTGGACAAAACTTAAGGAGAAAGACTTGTCTACAAAACATGTCACTTCTTCGAAGCCAAAATCTCTGACCACGAAGGAATCGTCATCTCCTGATACTTCAGGAGGTTCAGAGGGGATAAGAAACAGTAATTCAAAGGTAGGCCAGAGGACAGTCACCACTCTGAAGCCAAAATCTTTGGCTGCGAAGCAAATTTCTTCTCTTGACTCTTCAGGAGGCTTAAATGGAGATGGAAAAAGTGAGGCGAAGATAGGCAAGAGGGCAGGAACGTCCTTTGTTGCTTTAAAGAAAGTATTGGTGCCCCCAACAGGATCATTGTCCCCAAAACCTTCTCTCAGGAGAGTTGCAAGCTTAAAAGCACAGGACAGAAATGTGAAAGTTACTCCTCTTAAGAATCAGAACAAGATCGCAGAAGTTATAGCTAAGCAACTCAACAATGATGAGACTCCGGAGAAGACCTTGTATGTCATTAAGATTGAGACAGAGAACAAACCTCTGGAATCTGATCAAAACAAAAATTGTGAAGGTCCACCACCTTCATCCTCCTCATCATCGCCCAAATCTTTGTCCCTCCCAAACAGCATGTCCCTTTCACCCCTTGAAGGAGAAGATCAAGAGTCTGAATATACAGTGACAGAAACAGAAGAGGAGTCTTTCTCAGAAGACGATGAAGTTGACAACGAAGAAAATGCAGAGACCCTAGATGAGGATTACAAAGGGAAGCCCAGAAAGTCTGGGATGGTTTGTTCTGAAGATGTGGATAGCCATCCTTTAAAGTTTAGGAGGGGAAAGGTGGTTGACATGCAATTTCAGAATAATAGACCAAGGAGACTCAAATTTAGGCGAGGAAAAGTGTTGGGAGAGAGCGAAAATGTCAAGGCTGATGCCCAAAGGCGAAGATATAAGAAGAGAGAAGGTGTTGATGGCGATGCAATTGGTACCAAACCTGGTGCAGAAAAGGTTGTTTTGAGACATCAAGATGTTCAGGGGAAGAAAGATGAGAAGGGGTTGTTTAATAATGTAATTGAAGAAACTGCAAGTAAACTGGTTGAAACCCGGAAGAGTAAGGTCAAGGCCTTAGTTGGTGCTTTTGAAACAGTGATCTCACTTCAAGAGTGCAAACCTTCTGCAAATTCGGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

561

Amino Acids

61.4

Weight (kDa)

9.63

Isoelectric Point (pI)

52.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 438 - 549 8.3e-36 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1478
AccB1I GGYRCC 2 cut(s) 838, 1478
AccI GTMKAC 1 cut(s) 572
AccIII TCCGGA 1 cut(s) 988
AciI CCGC 1 cut(s) 520
AclWI GGATC 1 cut(s) 859
AcsI RAATTY 5 cut(s) 46, 524, 732, 1376, 1672
AcuI CTGAAG 4 cut(s) 624, 725, 735, 1311
AfaI GTAC 4 cut(s) 18, 192, 328, 1480
AfiI CCNNNNNNNGG 4 cut(s) 184, 848, 1142, 1246
AflII CTTAAG 2 cut(s) 554, 929
AflIII ACRYGT 2 cut(s) 323, 580
AgsI TTSAA 9 cut(s) 161, 439, 534, 675, 1142, 1568, 1592, 1634, 1652
AjnI CCWGG 1 cut(s) 1486
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AloI GAACNNNNNNTCC 4 cut(s) 72, 104, 1270, 1302
AluBI AGCT 5 cut(s) 283, 371, 400, 891, 962
AluI AGCT 5 cut(s) 283, 371, 400, 891, 962
Alw26I GTCTC 6 cut(s) 34, 977, 1013, 1229, 1363, 1504
AlwI GGATC 1 cut(s) 859
AlwNI CAGNNNCTG 1 cut(s) 355
Ama87I CYCGRG 1 cut(s) 296
Aor13HI TCCGGA 1 cut(s) 988
AoxI GGCC 2 cut(s) 682, 1614
ApeKI GCWGC 2 cut(s) 349, 722
ApoI RAATTY 5 cut(s) 46, 524, 732, 1376, 1672
ArsI GACNNNNNNTTYG 4 cut(s) 608, 640, 1109, 1141
Asp700I GAANNNNTTC 1 cut(s) 87
Asp718I GGTACC 1 cut(s) 1478
AspS9I GGNCC 1 cut(s) 1067
AsuC2I CCSGG 1 cut(s) 1597
AsuHPI GGTGA 5 cut(s) 285, 306, 442, 688, 1125
AsuII TTCGAA 1 cut(s) 593
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 1 cut(s) 1067
BaeGI GKGCMC 2 cut(s) 480, 843
BanI GGYRCC 2 cut(s) 838, 1478
BbsI GAAGAC 3 cut(s) 205, 1001, 1209
BbvI GCAGC 2 cut(s) 361, 709
BccI CCATC 6 cut(s) 338, 430, 764, 1273, 1314, 1457
BcgI CGANNNNNNTGC 2 cut(s) 1211, 1245
BciT130I CCWGG 1 cut(s) 1488
BclI TGATCA 2 cut(s) 276, 1045
BcnI CCSGG 1 cut(s) 1597
BcoDI GTCTC 6 cut(s) 34, 977, 1013, 1229, 1363, 1504
BfaI CTAG 1 cut(s) 1241
BfmI CTRYAG 1 cut(s) 51
BfrI CTTAAG 2 cut(s) 554, 929
BglI GCCNNNNNGGC 1 cut(s) 341
BisI GCNGC 2 cut(s) 350, 723
BlpI GCTNAGC 2 cut(s) 345, 963
BlsI GCNGC 2 cut(s) 351, 724
Bme1390I CCNGG 2 cut(s) 1488, 1597
Bme18I GGWCC 1 cut(s) 1067
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 1067
BmiI GGNNCC 3 cut(s) 118, 840, 1480
BmrFI CCNGG 2 cut(s) 1488, 1597
BmsI GCATC 4 cut(s) 142, 164, 1414, 1459
BpiI GAAGAC 3 cut(s) 205, 1001, 1209
BplI GAGNNNNNCTC 2 cut(s) 1181, 1213
Bpu1102I GCTNAGC 2 cut(s) 345, 963
Bpu14I TTCGAA 1 cut(s) 593
BpuMI CCSGG 1 cut(s) 1597
BsaBI GATNNNNATC 1 cut(s) 1305
BsaI GGTCTC 1 cut(s) 1229
BsaJI CCNNGG 1 cut(s) 1364
BsaWI WCCGGW 1 cut(s) 988
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 848, 1142, 1246
Bse1I ACTGG 2 cut(s) 118, 1590
Bse8I GATNNNNATC 1 cut(s) 1305
BseAI TCCGGA 1 cut(s) 988
BseBI CCWGG 1 cut(s) 1488
BseDI CCNNGG 1 cut(s) 1364
BseGI GGATG 4 cut(s) 446, 1079, 1284, 1306
BseJI GATNNNNATC 1 cut(s) 1305
BseLI CCNNNNNNNGG 4 cut(s) 184, 848, 1142, 1246
BseMII CTCAG 5 cut(s) 115, 246, 399, 889, 1212
BseNI ACTGG 2 cut(s) 118, 1590
BseRI GAGGAG 3 cut(s) 915, 1075, 1202
BseSI GKGCMC 2 cut(s) 480, 843
BseXI GCAGC 2 cut(s) 361, 709
BsgI GTGCAG 1 cut(s) 1512
BshFI GGCC 2 cut(s) 684, 1616
BshNI GGYRCC 2 cut(s) 838, 1478
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 2 cut(s) 989, 1597
BslFI GGGAC 3 cut(s) 844, 1093, 1111
BslI CCNNNNNNNGG 4 cut(s) 184, 848, 1142, 1246
BsmAI GTCTC 6 cut(s) 34, 977, 1013, 1229, 1363, 1504
BsmBI CGTCTC 1 cut(s) 34
BsmFI GGGAC 3 cut(s) 844, 1093, 1111
BsnI GGCC 2 cut(s) 684, 1616
Bso31I GGTCTC 1 cut(s) 1229
BsoBI CYCGRG 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 593
Bsp1286I GDGCHC 2 cut(s) 480, 843
Bsp13I TCCGGA 1 cut(s) 988
Bsp143I GATC 7 cut(s) 258, 276, 851, 945, 1045, 1150, 1641
Bsp1720I GCTNAGC 2 cut(s) 345, 963
BspACI CCGC 1 cut(s) 520
BspANI GGCC 2 cut(s) 684, 1616
BspCNI CTCAG 5 cut(s) 114, 245, 400, 888, 1211
BspEI TCCGGA 1 cut(s) 988
BspHI TCATGA 1 cut(s) 124
BspLI GGNNCC 3 cut(s) 118, 840, 1480
BspPI GGATC 1 cut(s) 859
BspT104I TTCGAA 1 cut(s) 593
BspT107I GGYRCC 2 cut(s) 838, 1478
BspTI CTTAAG 2 cut(s) 554, 929
BspTNI GGTCTC 1 cut(s) 1229
BsrI ACTGG 2 cut(s) 118, 1590
BssECI CCNNGG 1 cut(s) 1364
BssMI GATC 7 cut(s) 258, 276, 851, 945, 1045, 1150, 1641
BssT1I CCWWGG 1 cut(s) 1364
Bst2UI CCWGG 1 cut(s) 1488
Bst4CI ACNGT 5 cut(s) 487, 668, 694, 1171, 1639
Bst6I CTCTTC 6 cut(s) 156, 535, 754, 1179, 1442, 1595
BstAFI CTTAAG 2 cut(s) 554, 929
BstAPI GCANNNNNTGC 1 cut(s) 355
BstBI TTCGAA 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 342, 889
BstDEI CTNAG 8 cut(s) 101, 232, 345, 408, 875, 963, 1198, 1618
BstENI CCTNNNNNAGG 1 cut(s) 1244
BstF5I GGATG 4 cut(s) 446, 1079, 1284, 1306
BstKTI GATC 7 cut(s) 261, 279, 854, 948, 1048, 1153, 1644
BstMAI GTCTC 6 cut(s) 34, 977, 1013, 1229, 1363, 1504
BstMBI GATC 7 cut(s) 258, 276, 851, 945, 1045, 1150, 1641
BstMWI GCNNNNNNNGC 3 cut(s) 341, 346, 355
BstNI CCWGG 1 cut(s) 1488
BstNSI RCATGY 4 cut(s) 327, 584, 1126, 1345
BstSCI CCNGG 2 cut(s) 1486, 1595
BstSFI CTRYAG 1 cut(s) 51
BstSLI GKGCMC 2 cut(s) 480, 843
BstV1I GCAGC 2 cut(s) 361, 709
BstV2I GAAGAC 3 cut(s) 205, 1001, 1209
BsuRI GGCC 2 cut(s) 684, 1616
BtgZI GCGATG 3 cut(s) 316, 1077, 1482
BtsCI GGATG 4 cut(s) 446, 1079, 1284, 1306
BtsIMutI CAGTG 6 cut(s) 19, 82, 111, 279, 1176, 1644
Cac8I GCNNGC 2 cut(s) 342, 889
CaiI CAGNNNCTG 1 cut(s) 355
CciI TCATGA 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 1067
CsiI ACCWGGT 1 cut(s) 1486
Csp6I GTAC 4 cut(s) 17, 191, 327, 1479
CviAII CATG 5 cut(s) 125, 324, 581, 1123, 1342
CviQI GTAC 4 cut(s) 17, 191, 327, 1479
DdeI CTNAG 8 cut(s) 101, 232, 345, 408, 875, 963, 1198, 1618
DpnI GATC 7 cut(s) 260, 278, 853, 947, 1047, 1152, 1643
DpnII GATC 7 cut(s) 258, 276, 851, 945, 1045, 1150, 1641
DraI TTTAAA 2 cut(s) 825, 1314
Eam1104I CTCTTC 6 cut(s) 156, 535, 754, 1179, 1442, 1595
EarI CTCTTC 6 cut(s) 156, 535, 754, 1179, 1442, 1595
Eco130I CCWWGG 1 cut(s) 1364
Eco147I AGGCCT 1 cut(s) 1616
Eco31I GGTCTC 1 cut(s) 1229
Eco47I GGWCC 1 cut(s) 1067
Eco57I CTGAAG 4 cut(s) 624, 725, 735, 1311
Eco88I CYCGRG 1 cut(s) 296
EcoNI CCTNNNNNAGG 1 cut(s) 1244
EcoRII CCWGG 1 cut(s) 1486
EcoT14I CCWWGG 1 cut(s) 1364
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 1364
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 5 cut(s) 128, 327, 584, 1126, 1345
FaqI GGGAC 3 cut(s) 844, 1093, 1111
FatI CATG 5 cut(s) 124, 323, 580, 1122, 1341
FbaI TGATCA 2 cut(s) 276, 1045
FblI GTMKAC 1 cut(s) 572
Fnu4HI GCNGC 2 cut(s) 350, 723
FokI GGATG 4 cut(s) 433, 1066, 1291, 1293
Fsp4HI GCNGC 2 cut(s) 350, 723
FspBI CTAG 1 cut(s) 1241
GluI GCNGC 2 cut(s) 350, 723
HaeIII GGCC 2 cut(s) 684, 1616
HapII CCGG 2 cut(s) 989, 1597
Hin1II CATG 5 cut(s) 128, 327, 584, 1126, 1345
HincII GTYRAC 2 cut(s) 1216, 1339
HindII GTYRAC 2 cut(s) 1216, 1339
HindIII AAGCTT 1 cut(s) 889
HpaII CCGG 2 cut(s) 989, 1597
HphI GGTGA 5 cut(s) 285, 306, 442, 688, 1125
Hpy166II GTNNAC 6 cut(s) 17, 573, 1070, 1216, 1339, 1583
Hpy188I TCNGA 9 cut(s) 609, 652, 705, 939, 1045, 1162, 1201, 1291, 1353
Hpy8I GTNNAC 6 cut(s) 17, 573, 1070, 1216, 1339, 1583
HpyAV CCTTC 9 cut(s) 160, 610, 879, 1058, 1086, 1136, 1448, 1537, 1674
HpyCH4III ACNGT 5 cut(s) 487, 668, 694, 1171, 1639
HpyCH4IV ACGT 2 cut(s) 443, 809
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 346, 355
HpyF3I CTNAG 8 cut(s) 101, 232, 345, 408, 875, 963, 1198, 1618
HpySE526I ACGT 2 cut(s) 443, 809
Hsp92II CATG 5 cut(s) 128, 327, 584, 1126, 1345
Kpn2I TCCGGA 1 cut(s) 988
KpnI GGTACC 1 cut(s) 1482
Ksp22I TGATCA 2 cut(s) 276, 1045
Kzo9I GATC 7 cut(s) 258, 276, 851, 945, 1045, 1150, 1641
Lsp1109I GCAGC 2 cut(s) 361, 709
LweI GCATC 4 cut(s) 142, 164, 1414, 1459
MabI ACCWGGT 1 cut(s) 1486
MaeI CTAG 1 cut(s) 1241
MaeII ACGT 2 cut(s) 443, 809
MaeIII GTNAC 4 cut(s) 583, 694, 919, 1171
MalI GATC 7 cut(s) 260, 278, 853, 947, 1047, 1152, 1643
MboI GATC 7 cut(s) 258, 276, 851, 945, 1045, 1150, 1641
MfeI CAATTG 1 cut(s) 1473
MhlI GDGCHC 2 cut(s) 480, 843
MlyI GAGTC 7 cut(s) 195, 308, 740, 979, 1166, 1199, 1365
MmeI TCCRAC 1 cut(s) 524
Mph1103I ATGCAT 1 cut(s) 157
MroI TCCGGA 1 cut(s) 988
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 8 cut(s) 555, 761, 824, 893, 930, 1011, 1313, 1554
MslI CAYNNNNRTG 1 cut(s) 1518
MspA1I CMGCKG 1 cut(s) 371
MspCI CTTAAG 2 cut(s) 554, 929
MspI CCGG 2 cut(s) 989, 1597
MspR9I CCNGG 2 cut(s) 1488, 1597
MunI CAATTG 1 cut(s) 1473
MvaI CCWGG 1 cut(s) 1488
MwoI GCNNNNNNNGC 3 cut(s) 341, 346, 355
NciI CCSGG 1 cut(s) 1597
NdeII GATC 7 cut(s) 258, 276, 851, 945, 1045, 1150, 1641
NlaIII CATG 5 cut(s) 128, 327, 584, 1126, 1345
NlaIV GGNNCC 3 cut(s) 118, 840, 1480
NmuCI GTSAC 3 cut(s) 583, 694, 1171
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 4 cut(s) 327, 584, 1126, 1345
NspV TTCGAA 1 cut(s) 593
PagI TCATGA 1 cut(s) 124
PceI AGGCCT 1 cut(s) 1616
PciI ACATGT 2 cut(s) 323, 580
PcsI WCGNNNNNNNCGW 1 cut(s) 620
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 3 cut(s) 620, 934, 1040
PkrI GCNGC 2 cut(s) 351, 724
PleI GAGTC 7 cut(s) 195, 307, 740, 979, 1165, 1198, 1365
PpsI GAGTC 7 cut(s) 195, 307, 740, 979, 1165, 1198, 1365
PscI ACATGT 2 cut(s) 323, 580
Psp6I CCWGG 1 cut(s) 1486
PspGI CCWGG 1 cut(s) 1486
PspN4I GGNNCC 3 cut(s) 118, 840, 1480
PspPI GGNCC 1 cut(s) 1067
PstNI CAGNNNCTG 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 4 cut(s) 18, 192, 328, 1480
RsaNI GTAC 4 cut(s) 17, 191, 327, 1479
RseI CAYNNNNRTG 1 cut(s) 1518
SaqAI TTAA 8 cut(s) 555, 761, 824, 893, 930, 1011, 1313, 1554
SatI GCNGC 2 cut(s) 350, 723
Sau3AI GATC 7 cut(s) 258, 276, 851, 945, 1045, 1150, 1641
Sau96I GGNCC 1 cut(s) 1067
SchI GAGTC 7 cut(s) 195, 308, 740, 979, 1166, 1199, 1365
ScrFI CCNGG 2 cut(s) 1488, 1597
SduI GDGCHC 2 cut(s) 480, 843
SexAI ACCWGGT 1 cut(s) 1486
SfaNI GCATC 4 cut(s) 142, 164, 1414, 1459
SfcI CTRYAG 1 cut(s) 51
SfuI TTCGAA 1 cut(s) 593
SinI GGWCC 1 cut(s) 1067
SmiMI CAYNNNNRTG 1 cut(s) 1518
SmlI CTYRAG 2 cut(s) 554, 929
SmoI CTYRAG 2 cut(s) 554, 929
SseBI AGGCCT 1 cut(s) 1616
SsiI CCGC 1 cut(s) 520
SspMI CTAG 1 cut(s) 1241
StuI AGGCCT 1 cut(s) 1616
StyD4I CCNGG 2 cut(s) 1486, 1595
StyI CCWWGG 1 cut(s) 1364
TaaI ACNGT 5 cut(s) 487, 668, 694, 1171, 1639
TaiI ACGT 2 cut(s) 446, 812
TaqI TCGA 2 cut(s) 38, 593
TfiI GAWTC 3 cut(s) 620, 934, 1040
Tru1I TTAA 8 cut(s) 555, 761, 824, 893, 930, 1011, 1313, 1554
Tru9I TTAA 8 cut(s) 555, 761, 824, 893, 930, 1011, 1313, 1554
TscAI CASTG 6 cut(s) 19, 82, 118, 279, 1176, 1644
TseFI GTSAC 3 cut(s) 583, 694, 1171
TseI GCWGC 2 cut(s) 349, 722
Tsp45I GTSAC 3 cut(s) 583, 694, 1171
TspDTI ATGAA 3 cut(s) 492, 1068, 1224
TspRI CASTG 6 cut(s) 19, 82, 118, 279, 1176, 1644
Vha464I CTTAAG 2 cut(s) 554, 929
VpaK11BI GGWCC 1 cut(s) 1067
XagI CCTNNNNNAGG 1 cut(s) 1244
XapI RAATTY 5 cut(s) 46, 524, 732, 1376, 1672
XceI RCATGY 4 cut(s) 327, 584, 1126, 1345
XmiI GTMKAC 1 cut(s) 572
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 1 cut(s) 1241
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.