RLG00000023838

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005380
Physical Location & Seq
Forward (+)
29015172 .. 29016216
1045 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023838

Sequence Viewer

Length: 618 bp
ATGACGAGACTCCGGAGAAGACCTTGTATGTCATTAAGATTGAGACTGAGATCTGATCAAAACAAAAATTGTGAAGGTCCATCACCACCTTCATCCTCCTCATCATCGCCCAAGTCCTTGTCCCTCCCAAACAGCATGTCCCTTTCACCCCATGTAGGAGAAGATCAAGAGTCTGAATATACAATGACAGAAACAGAAGAGGACTCTTTCTCAAAAGACAATGAAGTTGACAACGAAGAGAATGCAGAGACCCTAGATGAGGATTACAAAGGGAAGCCCAGAAAGTCTGGGATGGTTTGTTCTGAAGATGTGGATAGCCATCCTTTAAAGTTCAGCAGGGGAAAGGGGAAGAAAGATGAGAAGGGGTTGTTTAATAATGTAATTGAAGAAACTGCAAGTAAACTGGTTGAAACCCGAAGGAGTAAGGTCAAGGCCTTCGTTGATGCTTTTGAAACAGTGATCTCACTTCAAGAGTGCAAACCTTCAGCAAATTCTGGGTTTTTTTTCTTTTCTGGTTTCGTCGCCATCACCGTCTTTCTCGCTCGGTCACTTTTTCGGCAACTCCCTCGACAAATCCACCAAACCACTCACCATTGCCGTCTTCGTGGTCGGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

206

Amino Acids

23.2

Weight (kDa)

8.26

Isoelectric Point (pI)

69.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 61 - 155 7.5e-19 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 12
AcsI RAATTY 1 cut(s) 490
AcuI CTGAAG 2 cut(s) 324, 468
AfiI CCNNNNNNNGG 2 cut(s) 155, 259
AgsI TTSAA 4 cut(s) 386, 410, 452, 470
AloI GAACNNNNNNTCC 2 cut(s) 283, 315
Alw26I GTCTC 2 cut(s) 37, 242
Aor13HI TCCGGA 1 cut(s) 12
AoxI GGCC 1 cut(s) 432
ApoI RAATTY 1 cut(s) 490
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 4 cut(s) 75, 138, 520, 581
AvaII GGWCC 1 cut(s) 77
BbsI GAAGAC 2 cut(s) 25, 593
BccI CCATC 4 cut(s) 88, 286, 327, 533
BceAI ACGGC 1 cut(s) 582
BcgI CGANNNNNNTGC 4 cut(s) 224, 258, 548, 582
BclI TGATCA 1 cut(s) 55
BcoDI GTCTC 2 cut(s) 37, 242
BfaI CTAG 2 cut(s) 254, 616
BglII AGATCT 1 cut(s) 50
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 77
BmsI GCATC 1 cut(s) 433
BpiI GAAGAC 2 cut(s) 25, 593
BsaBI GATNNNNATC 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 242
BsaWI WCCGGW 1 cut(s) 12
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 259
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 592
Bse8I GATNNNNATC 1 cut(s) 318
BseAI TCCGGA 1 cut(s) 12
BseGI GGATG 3 cut(s) 92, 297, 319
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 2 cut(s) 155, 259
BseMI GCAATG 1 cut(s) 592
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 1 cut(s) 88
BshFI GGCC 1 cut(s) 434
BsiSI CCGG 1 cut(s) 13
BslFI GGGAC 2 cut(s) 106, 124
BslI CCNNNNNNNGG 2 cut(s) 155, 259
BsmAI GTCTC 2 cut(s) 37, 242
BsmFI GGGAC 2 cut(s) 106, 124
BsmI GAATGC 1 cut(s) 247
BsnI GGCC 1 cut(s) 434
Bso31I GGTCTC 1 cut(s) 242
Bsp13I TCCGGA 1 cut(s) 12
Bsp143I GATC 4 cut(s) 50, 55, 163, 459
BspANI GGCC 1 cut(s) 434
BspCNI CTCAG 1 cut(s) 39
BspEI TCCGGA 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 592
BsrI ACTGG 1 cut(s) 408
BssMI GATC 4 cut(s) 50, 55, 163, 459
Bst4CI ACNGT 2 cut(s) 457, 532
Bst6I CTCTTC 2 cut(s) 192, 231
BstDEI CTNAG 1 cut(s) 47
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 3 cut(s) 92, 297, 319
BstKTI GATC 4 cut(s) 53, 58, 166, 462
BstMAI GTCTC 2 cut(s) 37, 242
BstMBI GATC 4 cut(s) 50, 55, 163, 459
BstNSI RCATGY 1 cut(s) 139
BstV2I GAAGAC 2 cut(s) 25, 593
BstX2I RGATCY 1 cut(s) 50
BstYI RGATCY 1 cut(s) 50
BsuRI GGCC 1 cut(s) 434
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 3 cut(s) 92, 297, 319
BtsIMutI CAGTG 1 cut(s) 462
Cfr13I GGNCC 1 cut(s) 77
CviAII CATG 2 cut(s) 136, 152
CviJI RGCY 3 cut(s) 277, 318, 434
CviKI_1 RGCY 3 cut(s) 277, 318, 434
DdeI CTNAG 1 cut(s) 47
DpnI GATC 4 cut(s) 52, 57, 165, 461
DpnII GATC 4 cut(s) 50, 55, 163, 459
DraI TTTAAA 1 cut(s) 327
Eam1104I CTCTTC 2 cut(s) 192, 231
EarI CTCTTC 2 cut(s) 192, 231
Eco147I AGGCCT 1 cut(s) 434
Eco31I GGTCTC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 77
Eco57I CTGAAG 2 cut(s) 324, 468
EcoNI CCTNNNNNAGG 1 cut(s) 257
FaeI CATG 2 cut(s) 139, 155
FaiI YATR 4 cut(s) 29, 137, 153, 180
FaqI GGGAC 2 cut(s) 106, 124
FatI CATG 2 cut(s) 135, 151
FbaI TGATCA 1 cut(s) 55
FokI GGATG 3 cut(s) 79, 304, 306
FspBI CTAG 2 cut(s) 254, 616
HaeIII GGCC 1 cut(s) 434
HapII CCGG 1 cut(s) 13
Hin1II CATG 2 cut(s) 139, 155
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 3 cut(s) 9, 170, 203
HpaII CCGG 1 cut(s) 13
HphI GGTGA 4 cut(s) 75, 138, 520, 581
Hpy166II GTNNAC 2 cut(s) 229, 401
Hpy188I TCNGA 3 cut(s) 55, 175, 304
Hpy188III TCNNGA 3 cut(s) 13, 167, 470
Hpy8I GTNNAC 2 cut(s) 229, 401
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 6 cut(s) 68, 99, 355, 411, 445, 492
HpyCH4III ACNGT 2 cut(s) 457, 532
HpyCH4V TGCA 3 cut(s) 245, 395, 477
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 2 cut(s) 139, 155
Kpn2I TCCGGA 1 cut(s) 12
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 4 cut(s) 50, 55, 163, 459
LpnPI CCDG 7 cut(s) 26, 273, 292, 322, 389, 480, 498
LweI GCATC 1 cut(s) 433
MaeI CTAG 2 cut(s) 254, 616
MaeIII GTNAC 1 cut(s) 546
MalI GATC 4 cut(s) 52, 57, 165, 461
MboI GATC 4 cut(s) 50, 55, 163, 459
MboII GAAGA 8 cut(s) 30, 173, 209, 248, 317, 361, 398, 593
MflI RGATCY 1 cut(s) 50
MluCI AATT 3 cut(s) 67, 381, 490
MlyI GAGTC 3 cut(s) 3, 179, 197
MnlI CCTC 6 cut(s) 106, 109, 134, 193, 253, 576
MroI TCCGGA 1 cut(s) 12
MseI TTAA 3 cut(s) 35, 326, 372
MspI CCGG 1 cut(s) 13
Mva1269I GAATGC 1 cut(s) 247
NdeII GATC 4 cut(s) 50, 55, 163, 459
NlaIII CATG 2 cut(s) 139, 155
NmuCI GTSAC 1 cut(s) 546
NspI RCATGY 1 cut(s) 139
PceI AGGCCT 1 cut(s) 434
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PctI GAATGC 1 cut(s) 247
PleI GAGTC 3 cut(s) 3, 178, 197
PpsI GAGTC 3 cut(s) 3, 178, 197
PspPI GGNCC 1 cut(s) 77
PsuI RGATCY 1 cut(s) 50
SaqAI TTAA 3 cut(s) 35, 326, 372
Sau3AI GATC 4 cut(s) 50, 55, 163, 459
Sau96I GGNCC 1 cut(s) 77
SchI GAGTC 3 cut(s) 3, 179, 197
SetI ASST 5 cut(s) 25, 79, 91, 429, 484
SfaNI GCATC 1 cut(s) 433
SinI GGWCC 1 cut(s) 77
Sse9I AATT 3 cut(s) 67, 381, 490
SseBI AGGCCT 1 cut(s) 434
SspMI CTAG 2 cut(s) 254, 616
StuI AGGCCT 1 cut(s) 434
TaaI ACNGT 2 cut(s) 457, 532
TaqI TCGA 1 cut(s) 568
TaqII GACCGA 1 cut(s) 534
TasI AATT 3 cut(s) 67, 381, 490
Tru1I TTAA 3 cut(s) 35, 326, 372
Tru9I TTAA 3 cut(s) 35, 326, 372
TscAI CASTG 1 cut(s) 462
TseFI GTSAC 1 cut(s) 546
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 2 cut(s) 81, 237
TspRI CASTG 1 cut(s) 462
VpaK11BI GGWCC 1 cut(s) 77
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 1 cut(s) 490
XceI RCATGY 1 cut(s) 139
XspI CTAG 2 cut(s) 254, 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.