Rorug02G0524000

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005380
Physical Location & Seq
Reverse (-)
65459613 .. 65461632
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0524000.1

Sequence Viewer

Length: 579 bp
ATGGCGATGAGTCCGGCGGTGCTGAAGGCGGGGCTGGTCATAGTGGGCCTCTGCATAGCCGGCTACATTCTCGGCCCACCTCTCTACTGGCACTTCATGGAGGGCTTGGCCGCCGTCTCCCGCTCTTCTTCTTCTTATTCTTATTCTTATTCCTGCCCTCCTTGCACCTGCGACTGCTCTTCTCAGCCGCTCCTCTCCATCCCCATTGGACTCAGCAACATTTCGTTTGCAGACTGTGCAAAGCATGATCCAGAGGTGAATGAGGATACTGAAAAGAACTTTGTGGATCTTTTGACTGAGGAATTGAAGCTTCGGGAAGCTCAAGCTTTGGAGAATCAGCAAAAAGCTGATATAACTCTACTGGAGGCTAAGAAGATTGCATCCCAGTATCAAAAGGAAGCTGACAAGTGCAGTTCAGGGATGGAAACATGTGAAGAAGCAAGGGAGAAAGCTGAAGCAACATTAGAGGCTCAGAGGAAAATGACAGCAACGTGGGAGCTTAGGGCTCGTCAAAAGGGATGGAAAGAAGGAATGACCAAGTCTACTGCTCAGACTCAAGGAAATGTCCAGACTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

192

Amino Acids

21.05

Weight (kDa)

5.19

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1068 PF06364 6 - 177 2.3e-82 Protein of unknown function (DUF1068)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 176
Acc36I ACCTGC 1 cut(s) 176
AccBSI CCGCTC 2 cut(s) 123, 190
AccI GTMKAC 1 cut(s) 542
AciI CCGC 5 cut(s) 17, 29, 111, 121, 188
AclWI GGATC 2 cut(s) 242, 294
AcoI YGGCCR 1 cut(s) 108
AcuI CTGAAG 2 cut(s) 44, 474
AflIII ACRYGT 1 cut(s) 428
AgsI TTSAA 1 cut(s) 307
AluBI AGCT 7 cut(s) 310, 320, 326, 347, 401, 452, 499
AluI AGCT 7 cut(s) 310, 320, 326, 347, 401, 452, 499
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 2 cut(s) 242, 294
AoxI GGCC 3 cut(s) 46, 73, 108
AspS9I GGNCC 2 cut(s) 46, 74
AsuHPI GGTGA 1 cut(s) 268
BanII GRGCYC 1 cut(s) 508
BccI CCATC 3 cut(s) 206, 415, 513
BceAI ACGGC 1 cut(s) 98
BciVI GTATCC 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 121
BfuAI ACCTGC 1 cut(s) 176
BfuI GTATCC 1 cut(s) 259
BisI GCNGC 2 cut(s) 111, 188
BlsI GCNGC 2 cut(s) 112, 189
BmgT120I GGNCC 2 cut(s) 46, 74
BmrI ACTGGG 1 cut(s) 379
BmsI GCATC 1 cut(s) 389
BmuI ACTGGG 1 cut(s) 379
BpmI CTGGAG 1 cut(s) 383
Bpu10I CCTNAGC 1 cut(s) 500
BpuEI CTTGAG 2 cut(s) 306, 540
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 3 cut(s) 92, 366, 385
BseGI GGATG 4 cut(s) 198, 380, 426, 524
BseMII CTCAG 5 cut(s) 197, 226, 288, 485, 563
BseNI ACTGG 3 cut(s) 92, 366, 385
BseRI GAGGAG 1 cut(s) 182
BsgI GTGCAG 1 cut(s) 430
BshFI GGCC 3 cut(s) 48, 75, 110
BsiSI CCGG 2 cut(s) 14, 60
BsmAI GTCTC 1 cut(s) 121
BsmBI CGTCTC 1 cut(s) 121
BsnI GGCC 3 cut(s) 48, 75, 110
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 2 cut(s) 247, 286
BspACI CCGC 5 cut(s) 17, 29, 111, 121, 188
BspANI GGCC 3 cut(s) 48, 75, 110
BspCNI CTCAG 5 cut(s) 196, 225, 289, 484, 562
BspMI ACCTGC 1 cut(s) 176
BspPI GGATC 2 cut(s) 242, 294
BspQI GCTCTTC 2 cut(s) 130, 184
BsrBI CCGCTC 2 cut(s) 123, 190
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 3 cut(s) 92, 366, 385
BssAI RCCGGY 1 cut(s) 59
BssMI GATC 2 cut(s) 247, 286
Bst4CI ACNGT 1 cut(s) 236
Bst6I CTCTTC 2 cut(s) 130, 184
BstAPI GCANNNNNTGC 1 cut(s) 236
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 7 cut(s) 183, 212, 297, 369, 471, 500, 549
BstF5I GGATG 4 cut(s) 198, 380, 426, 524
BstKTI GATC 2 cut(s) 250, 289
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 2 cut(s) 247, 286
BstMWI GCNNNNNNNGC 3 cut(s) 60, 162, 236
BstNSI RCATGY 1 cut(s) 432
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 3 cut(s) 48, 75, 110
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 4 cut(s) 198, 380, 426, 524
BveI ACCTGC 1 cut(s) 176
Cac8I GCNNGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 2 cut(s) 46, 74
CviAII CATG 3 cut(s) 97, 245, 429
DdeI CTNAG 7 cut(s) 183, 212, 297, 369, 471, 500, 549
DpnI GATC 2 cut(s) 249, 288
DpnII GATC 2 cut(s) 247, 286
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 2 cut(s) 130, 184
EarI CTCTTC 2 cut(s) 130, 184
Eco24I GRGCYC 1 cut(s) 508
Eco57I CTGAAG 2 cut(s) 44, 474
EcoT38I GRGCYC 1 cut(s) 508
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 3 cut(s) 100, 248, 432
FaiI YATR 6 cut(s) 41, 56, 98, 246, 353, 430
FatI CATG 3 cut(s) 96, 244, 428
FauI CCCGC 2 cut(s) 22, 128
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 2 cut(s) 111, 188
FokI GGATG 4 cut(s) 185, 367, 433, 531
FriOI GRGCYC 1 cut(s) 508
Fsp4HI GCNGC 2 cut(s) 111, 188
GluI GCNGC 2 cut(s) 111, 188
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 3 cut(s) 48, 75, 110
HapII CCGG 2 cut(s) 14, 60
Hin1II CATG 3 cut(s) 100, 248, 432
HindIII AAGCTT 2 cut(s) 308, 324
HinfI GANTC 4 cut(s) 10, 210, 334, 553
HpaII CCGG 2 cut(s) 14, 60
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 1 cut(s) 543
Hpy188I TCNGA 2 cut(s) 474, 552
Hpy188III TCNNGA 3 cut(s) 251, 314, 568
Hpy8I GTNNAC 1 cut(s) 543
HpyAV CCTTC 2 cut(s) 19, 521
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4IV ACGT 1 cut(s) 491
HpyCH4V TGCA 6 cut(s) 54, 165, 230, 239, 380, 411
HpyF10VI GCNNNNNNNGC 3 cut(s) 60, 162, 236
HpyF3I CTNAG 7 cut(s) 183, 212, 297, 369, 471, 500, 549
HpySE526I ACGT 1 cut(s) 491
Hsp92II CATG 3 cut(s) 100, 248, 432
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Kzo9I GATC 2 cut(s) 247, 286
LguI GCTCTTC 2 cut(s) 130, 184
LmnI GCTCC 2 cut(s) 195, 496
LweI GCATC 1 cut(s) 389
MaeII ACGT 1 cut(s) 491
MalI GATC 2 cut(s) 249, 288
MbiI CCGCTC 2 cut(s) 123, 190
MboI GATC 2 cut(s) 247, 286
MboII GAAGA 6 cut(s) 117, 120, 123, 171, 385, 446
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 508
MluCI AATT 1 cut(s) 302
MlyI GAGTC 3 cut(s) 19, 204, 547
MroNI GCCGGC 1 cut(s) 59
MspI CCGG 2 cut(s) 14, 60
MwoI GCNNNNNNNGC 3 cut(s) 60, 162, 236
NaeI GCCGGC 1 cut(s) 61
NdeII GATC 2 cut(s) 247, 286
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 3 cut(s) 100, 248, 432
NmeAIII GCCGAG 1 cut(s) 51
NspI RCATGY 1 cut(s) 432
PaqCI CACCTGC 1 cut(s) 176
PciI ACATGT 1 cut(s) 428
PciSI GCTCTTC 2 cut(s) 130, 184
PdiI GCCGGC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 334
PflFI GACNNNGTC 1 cut(s) 538
PkrI GCNGC 2 cut(s) 112, 189
PleI GAGTC 3 cut(s) 18, 204, 547
PpsI GAGTC 3 cut(s) 18, 204, 547
PscI ACATGT 1 cut(s) 428
PspPI GGNCC 2 cut(s) 46, 74
PsuI RGATCY 1 cut(s) 286
PsyI GACNNNGTC 1 cut(s) 538
SapI GCTCTTC 2 cut(s) 130, 184
SatI GCNGC 2 cut(s) 111, 188
Sau3AI GATC 2 cut(s) 247, 286
Sau96I GGNCC 2 cut(s) 46, 74
SchI GAGTC 3 cut(s) 19, 204, 547
SduI GDGCHC 1 cut(s) 508
SfaNI GCATC 1 cut(s) 389
SmlI CTYRAG 2 cut(s) 321, 555
SmoI CTYRAG 2 cut(s) 321, 555
Sse9I AATT 1 cut(s) 302
SsiI CCGC 5 cut(s) 17, 29, 111, 121, 188
TaaI ACNGT 1 cut(s) 236
TaiI ACGT 1 cut(s) 494
TasI AATT 1 cut(s) 302
TauI GCSGC 2 cut(s) 113, 190
TfiI GAWTC 1 cut(s) 334
TspDTI ATGAA 1 cut(s) 85
Tth111I GACNNNGTC 1 cut(s) 538
XceI RCATGY 1 cut(s) 432
XcmI CCANNNNNNNNNTGG 1 cut(s) 84
XmiI GTMKAC 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.