Rw2G049170

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005380
Physical Location & Seq
Reverse (-)
76641305 .. 76645247
3943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049170.1

Sequence Viewer

Length: 1707 bp
ATGGCAAGTATCAGTGTACCAACGGCCCAAGAATTAACAAAGCCAGATGAGGCTAAATCGAGACGAAATTCTACAGGAAAGATATTATCGTCGAACAGTAGAGAAAAAGTTCCTCATTATCTCAGGGCTTCCACTGGTTCCTGTCATGATTTTTGTAAATATGGGAGGAAAGATGCATTTGAAGAGAAGAAAAGATGCCCCATACGGGTTGTACCAAGAAGACTCTCAACTAAATCACTTGGTAGCCAAAACTCAGCAGAAAGTGTAGTTTTGCCAGAGATCAAGAATATATCAGTGATCAAGCTCAATCATTCACCCGAGTCCAAAATCCTCTCACCTGATACATGTACCATCGCCAAGCAGGCCAAGCAGCAACTGCCAAAAAGGTCAGCTGACAGAAAAACTTCTGTTGGGAGTGAGCTTCTTGCTGAGAGGAAGAAGCCATCATTGGTGAAGTTCAAAACGTCATCCAATTCAAAGCCTCCTGTATCTGCTGTGCCCAAAACTATGAAGCAGGAAGTTTCATCATCTCCTGAAAAACTGAAAGTCTCTTCAAAGAAAGGTTGGACAAAACTTAAGGAGAAAGACTTGTCTACAAAACATGTCACTTCTTCGAAGCCAAAATCTCTGACCACGAAGGAATTGTCATCTCCTGATACTTCAGGAGGTTCAGAGGGGATAAGAAACAGTAATTCAAAGGTAGGCCAGAGGACAGTCACCTCTCTGAAGCCAAAATCTTTGGCTGCGAAGCAAATTTCTTCTCTTGACTCTTCAGGAGGCTTAAATGGAGATGGAAAAAGTGAGGCGAAGATAGGCAAGAGGGCAGGAATGTCCTTTGTTGCTTTAAAGAAAGTATTGGTGCCCCCAACAGGATCATTGTCCCCAAAACCTTCTCTCAGGAGAGTGGCAAGATTAAAAGCACAGGACAGAAATGTGAAAGTTACTCCTCTTAAGAATCAGAACAAGATCGGAGAAGTTATAGCTAAGCAACTCAACAATGATGAGACTCCGGAGAAGACCTTGTATGTCATTAAGATTGAGACAGAGAACAAACCTCTGGAATCTGATCAAAACAAAAATTGTGAAGGTCCACCACCTTCATCCTCCTCATCATCGCCCAAATCTTTGTCCCTCCCAAACAGCATGTCCCTTTCACCCCTTGAAGGAGAAGATCAAGAGTCTGAATATACAATGACAGAAACAGAAGAGGAGTCTTTCTCAGAAGACGATGAAGTTGACAACGAAGAAAATGCAGAGACCCTAGATGAGGATTACAAAGGGAAGCCCAGAAAGTCTGGGATGGTTTGTTCTGAAGATGTGGATAGCCATCCTTTAAAGTTTAGGAGGGGAAAGGTGGTTGACATGCAATTTCAGAATAATAGACCAAGGAGGCTCAAATTTAGGCGAGGAAAAGTGTTGGGAGAGAGCGAAAATGTCAAGGCTGATGCCCAAAGGCGAAGATATAAGAAGAGAGAAGGTGTTGATGGCGATGCAATTGGTACCAAACCTGGTGCAGAAAAGGTTGTTTTGAGACATCAAGATGTTCAGGGGAAGAAAGATGAGAAGGGGTTGTTTAATAATGTAATTGAAGAAACTGCAAGTAAACTGGTTGAAACCCGGAAGAGTAAGGTCAAGGCCTTAGTTGGTGCTTTTGAAACAGTAATCTCACTTCAAGAGTGCAAACCTTCTGCAAATTCGGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

568

Amino Acids

62.37

Weight (kDa)

9.68

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 445 - 556 8.5e-36 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1499
AccB1I GGYRCC 2 cut(s) 859, 1499
AccI GTMKAC 1 cut(s) 593
AccIII TCCGGA 1 cut(s) 1009
AclWI GGATC 1 cut(s) 880
AcsI RAATTY 4 cut(s) 67, 753, 1397, 1693
AcuI CTGAAG 4 cut(s) 645, 746, 756, 1332
AfaI GTAC 4 cut(s) 18, 213, 349, 1501
AfiI CCNNNNNNNGG 4 cut(s) 205, 869, 1163, 1267
AflII CTTAAG 2 cut(s) 575, 950
AflIII ACRYGT 2 cut(s) 344, 601
AjnI CCWGG 1 cut(s) 1507
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AloI GAACNNNNNNTCC 2 cut(s) 1291, 1323
AluBI AGCT 4 cut(s) 304, 392, 421, 983
AluI AGCT 4 cut(s) 304, 392, 421, 983
Alw26I GTCTC 6 cut(s) 55, 553, 998, 1034, 1250, 1525
AlwI GGATC 1 cut(s) 880
AlwNI CAGNNNCTG 1 cut(s) 376
Ama87I CYCGRG 1 cut(s) 317
Aor13HI TCCGGA 1 cut(s) 1009
AoxI GGCC 4 cut(s) 24, 363, 703, 1635
ApeKI GCWGC 2 cut(s) 370, 743
ApoI RAATTY 4 cut(s) 67, 753, 1397, 1693
ArsI GACNNNNNNTTYG 4 cut(s) 629, 661, 1130, 1162
Asp700I GAANNNNTTC 2 cut(s) 108, 403
Asp718I GGTACC 1 cut(s) 1499
AspS9I GGNCC 2 cut(s) 25, 1088
AsuC2I CCSGG 1 cut(s) 1618
AsuHPI GGTGA 5 cut(s) 306, 327, 463, 709, 1146
AsuII TTCGAA 1 cut(s) 614
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 1088
BaeGI GKGCMC 2 cut(s) 501, 864
BanI GGYRCC 2 cut(s) 859, 1499
BarI GAAGNNNNNNTAC 2 cut(s) 1653, 1685
BbsI GAAGAC 3 cut(s) 226, 1022, 1230
BbvI GCAGC 2 cut(s) 382, 730
BccI CCATC 6 cut(s) 359, 451, 785, 1294, 1335, 1478
BceAI ACGGC 1 cut(s) 39
BcgI CGANNNNNNTGC 2 cut(s) 1232, 1266
BciT130I CCWGG 1 cut(s) 1509
BclI TGATCA 2 cut(s) 297, 1066
BcnI CCSGG 1 cut(s) 1618
BcoDI GTCTC 6 cut(s) 55, 553, 998, 1034, 1250, 1525
BfaI CTAG 1 cut(s) 1262
BfmI CTRYAG 1 cut(s) 72
BfrI CTTAAG 2 cut(s) 575, 950
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 2 cut(s) 371, 744
BlpI GCTNAGC 1 cut(s) 984
BlsI GCNGC 2 cut(s) 372, 745
Bme1390I CCNGG 2 cut(s) 1509, 1618
Bme18I GGWCC 1 cut(s) 1088
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 2 cut(s) 25, 1088
BmiI GGNNCC 3 cut(s) 139, 861, 1501
BmrFI CCNGG 2 cut(s) 1509, 1618
BmsI GCATC 4 cut(s) 163, 185, 1435, 1480
BpiI GAAGAC 3 cut(s) 226, 1022, 1230
BplI GAGNNNNNCTC 2 cut(s) 1202, 1234
Bpu1102I GCTNAGC 1 cut(s) 984
Bpu14I TTCGAA 1 cut(s) 614
BpuMI CCSGG 1 cut(s) 1618
BsaBI GATNNNNATC 1 cut(s) 1326
BsaI GGTCTC 1 cut(s) 1250
BsaJI CCNNGG 1 cut(s) 1385
BsaWI WCCGGW 1 cut(s) 1009
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 869, 1163, 1267
Bse1I ACTGG 2 cut(s) 139, 1611
Bse8I GATNNNNATC 1 cut(s) 1326
BseAI TCCGGA 1 cut(s) 1009
BseBI CCWGG 1 cut(s) 1509
BseDI CCNNGG 1 cut(s) 1385
BseGI GGATG 4 cut(s) 467, 1100, 1305, 1327
BseJI GATNNNNATC 1 cut(s) 1326
BseLI CCNNNNNNNGG 4 cut(s) 205, 869, 1163, 1267
BseMII CTCAG 5 cut(s) 136, 267, 420, 910, 1233
BseNI ACTGG 2 cut(s) 139, 1611
BseRI GAGGAG 3 cut(s) 936, 1096, 1223
BseSI GKGCMC 2 cut(s) 501, 864
BseXI GCAGC 2 cut(s) 382, 730
BsgI GTGCAG 1 cut(s) 1533
BshFI GGCC 4 cut(s) 26, 365, 705, 1637
BshNI GGYRCC 2 cut(s) 859, 1499
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 2 cut(s) 1010, 1618
BslFI GGGAC 3 cut(s) 865, 1114, 1132
BslI CCNNNNNNNGG 4 cut(s) 205, 869, 1163, 1267
BsmAI GTCTC 6 cut(s) 55, 553, 998, 1034, 1250, 1525
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 3 cut(s) 865, 1114, 1132
BsnI GGCC 4 cut(s) 26, 365, 705, 1637
Bso31I GGTCTC 1 cut(s) 1250
BsoBI CYCGRG 1 cut(s) 317
Bsp119I TTCGAA 1 cut(s) 614
Bsp1286I GDGCHC 2 cut(s) 501, 864
Bsp13I TCCGGA 1 cut(s) 1009
Bsp143I GATC 6 cut(s) 279, 297, 872, 966, 1066, 1171
Bsp1720I GCTNAGC 1 cut(s) 984
BspANI GGCC 4 cut(s) 26, 365, 705, 1637
BspCNI CTCAG 5 cut(s) 135, 266, 421, 909, 1232
BspEI TCCGGA 1 cut(s) 1009
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 3 cut(s) 139, 861, 1501
BspPI GGATC 1 cut(s) 880
BspT104I TTCGAA 1 cut(s) 614
BspT107I GGYRCC 2 cut(s) 859, 1499
BspTI CTTAAG 2 cut(s) 575, 950
BspTNI GGTCTC 1 cut(s) 1250
BsrI ACTGG 2 cut(s) 139, 1611
BssECI CCNNGG 1 cut(s) 1385
BssMI GATC 6 cut(s) 279, 297, 872, 966, 1066, 1171
BssT1I CCWWGG 1 cut(s) 1385
Bst2UI CCWGG 1 cut(s) 1509
Bst4CI ACNGT 4 cut(s) 98, 689, 715, 1660
Bst6I CTCTTC 6 cut(s) 177, 556, 775, 1200, 1463, 1616
BstAFI CTTAAG 2 cut(s) 575, 950
BstAPI GCANNNNNTGC 1 cut(s) 376
BstBI TTCGAA 1 cut(s) 614
BstC8I GCNNGC 1 cut(s) 363
BstDEI CTNAG 7 cut(s) 122, 253, 429, 896, 984, 1219, 1639
BstENI CCTNNNNNAGG 1 cut(s) 1265
BstF5I GGATG 4 cut(s) 467, 1100, 1305, 1327
BstKTI GATC 6 cut(s) 282, 300, 875, 969, 1069, 1174
BstMAI GTCTC 6 cut(s) 55, 553, 998, 1034, 1250, 1525
BstMBI GATC 6 cut(s) 279, 297, 872, 966, 1066, 1171
BstMWI GCNNNNNNNGC 3 cut(s) 362, 367, 376
BstNI CCWGG 1 cut(s) 1509
BstNSI RCATGY 4 cut(s) 348, 605, 1147, 1366
BstSCI CCNGG 2 cut(s) 1507, 1616
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 2 cut(s) 501, 864
BstV1I GCAGC 2 cut(s) 382, 730
BstV2I GAAGAC 3 cut(s) 226, 1022, 1230
BsuRI GGCC 4 cut(s) 26, 365, 705, 1637
BtgZI GCGATG 3 cut(s) 337, 1098, 1503
BtsCI GGATG 4 cut(s) 467, 1100, 1305, 1327
BtsIMutI CAGTG 3 cut(s) 19, 132, 300
Cac8I GCNNGC 1 cut(s) 363
CaiI CAGNNNCTG 1 cut(s) 376
CciI TCATGA 1 cut(s) 145
Cfr13I GGNCC 2 cut(s) 25, 1088
CsiI ACCWGGT 1 cut(s) 1507
Csp6I GTAC 4 cut(s) 17, 212, 348, 1500
CviAII CATG 5 cut(s) 146, 345, 602, 1144, 1363
CviQI GTAC 4 cut(s) 17, 212, 348, 1500
DdeI CTNAG 7 cut(s) 122, 253, 429, 896, 984, 1219, 1639
DpnI GATC 6 cut(s) 281, 299, 874, 968, 1068, 1173
DpnII GATC 6 cut(s) 279, 297, 872, 966, 1066, 1171
DraI TTTAAA 2 cut(s) 846, 1335
Eam1104I CTCTTC 6 cut(s) 177, 556, 775, 1200, 1463, 1616
EarI CTCTTC 6 cut(s) 177, 556, 775, 1200, 1463, 1616
Eco130I CCWWGG 1 cut(s) 1385
Eco147I AGGCCT 1 cut(s) 1637
Eco31I GGTCTC 1 cut(s) 1250
Eco47I GGWCC 1 cut(s) 1088
Eco57I CTGAAG 4 cut(s) 645, 746, 756, 1332
Eco88I CYCGRG 1 cut(s) 317
EcoNI CCTNNNNNAGG 1 cut(s) 1265
EcoRII CCWGG 1 cut(s) 1507
EcoT14I CCWWGG 1 cut(s) 1385
EcoT22I ATGCAT 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 1385
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 5 cut(s) 149, 348, 605, 1147, 1366
FaqI GGGAC 3 cut(s) 865, 1114, 1132
FatI CATG 5 cut(s) 145, 344, 601, 1143, 1362
FbaI TGATCA 2 cut(s) 297, 1066
FblI GTMKAC 1 cut(s) 593
Fnu4HI GCNGC 2 cut(s) 371, 744
FokI GGATG 4 cut(s) 454, 1087, 1312, 1314
Fsp4HI GCNGC 2 cut(s) 371, 744
FspBI CTAG 1 cut(s) 1262
GluI GCNGC 2 cut(s) 371, 744
HaeIII GGCC 4 cut(s) 26, 365, 705, 1637
HapII CCGG 2 cut(s) 1010, 1618
Hin1II CATG 5 cut(s) 149, 348, 605, 1147, 1366
HincII GTYRAC 2 cut(s) 1237, 1360
HindII GTYRAC 2 cut(s) 1237, 1360
HinfI GANTC 8 cut(s) 222, 320, 767, 955, 1006, 1061, 1178, 1211
HpaII CCGG 2 cut(s) 1010, 1618
HphI GGTGA 5 cut(s) 306, 327, 463, 709, 1146
Hpy166II GTNNAC 6 cut(s) 17, 594, 1091, 1237, 1360, 1604
Hpy8I GTNNAC 6 cut(s) 17, 594, 1091, 1237, 1360, 1604
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 8 cut(s) 631, 900, 1079, 1107, 1157, 1469, 1558, 1695
HpyCH4III ACNGT 4 cut(s) 98, 689, 715, 1660
HpyCH4IV ACGT 1 cut(s) 464
HpyCH4V TGCA 8 cut(s) 176, 1253, 1366, 1493, 1514, 1598, 1680, 1691
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 367, 376
HpyF3I CTNAG 7 cut(s) 122, 253, 429, 896, 984, 1219, 1639
HpySE526I ACGT 1 cut(s) 464
Hsp92II CATG 5 cut(s) 149, 348, 605, 1147, 1366
Kpn2I TCCGGA 1 cut(s) 1009
KpnI GGTACC 1 cut(s) 1503
Ksp22I TGATCA 2 cut(s) 297, 1066
Kzo9I GATC 6 cut(s) 279, 297, 872, 966, 1066, 1171
Lsp1109I GCAGC 2 cut(s) 382, 730
LweI GCATC 4 cut(s) 163, 185, 1435, 1480
MabI ACCWGGT 1 cut(s) 1507
MaeI CTAG 1 cut(s) 1262
MaeII ACGT 1 cut(s) 464
MaeIII GTNAC 3 cut(s) 604, 715, 940
MalI GATC 6 cut(s) 281, 299, 874, 968, 1068, 1173
MboI GATC 6 cut(s) 279, 297, 872, 966, 1066, 1171
MfeI CAATTG 1 cut(s) 1494
MhlI GDGCHC 2 cut(s) 501, 864
MlyI GAGTC 6 cut(s) 216, 329, 761, 1000, 1187, 1220
MmeI TCCRAC 1 cut(s) 545
Mph1103I ATGCAT 1 cut(s) 178
MroI TCCGGA 1 cut(s) 1009
MroXI GAANNNNTTC 2 cut(s) 108, 403
MseI TTAA 9 cut(s) 35, 576, 782, 845, 914, 951, 1032, 1334, 1575
MslI CAYNNNNRTG 1 cut(s) 1539
MspA1I CMGCKG 1 cut(s) 392
MspCI CTTAAG 2 cut(s) 575, 950
MspI CCGG 2 cut(s) 1010, 1618
MspR9I CCNGG 2 cut(s) 1509, 1618
MunI CAATTG 1 cut(s) 1494
MvaI CCWGG 1 cut(s) 1509
MwoI GCNNNNNNNGC 3 cut(s) 362, 367, 376
NciI CCSGG 1 cut(s) 1618
NdeII GATC 6 cut(s) 279, 297, 872, 966, 1066, 1171
NlaIII CATG 5 cut(s) 149, 348, 605, 1147, 1366
NlaIV GGNNCC 3 cut(s) 139, 861, 1501
NmuCI GTSAC 2 cut(s) 604, 715
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 4 cut(s) 348, 605, 1147, 1366
NspV TTCGAA 1 cut(s) 614
PagI TCATGA 1 cut(s) 145
PceI AGGCCT 1 cut(s) 1637
PciI ACATGT 2 cut(s) 344, 601
PdmI GAANNNNTTC 2 cut(s) 108, 403
PfeI GAWTC 2 cut(s) 955, 1061
PkrI GCNGC 2 cut(s) 372, 745
PleI GAGTC 6 cut(s) 216, 328, 761, 1000, 1186, 1219
PpsI GAGTC 6 cut(s) 216, 328, 761, 1000, 1186, 1219
PscI ACATGT 2 cut(s) 344, 601
Psp6I CCWGG 1 cut(s) 1507
PspGI CCWGG 1 cut(s) 1507
PspN4I GGNNCC 3 cut(s) 139, 861, 1501
PspPI GGNCC 2 cut(s) 25, 1088
PstNI CAGNNNCTG 1 cut(s) 376
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 4 cut(s) 18, 213, 349, 1501
RsaNI GTAC 4 cut(s) 17, 212, 348, 1500
RseI CAYNNNNRTG 1 cut(s) 1539
SaqAI TTAA 9 cut(s) 35, 576, 782, 845, 914, 951, 1032, 1334, 1575
SatI GCNGC 2 cut(s) 371, 744
Sau3AI GATC 6 cut(s) 279, 297, 872, 966, 1066, 1171
Sau96I GGNCC 2 cut(s) 25, 1088
SchI GAGTC 6 cut(s) 216, 329, 761, 1000, 1187, 1220
ScrFI CCNGG 2 cut(s) 1509, 1618
SduI GDGCHC 2 cut(s) 501, 864
SexAI ACCWGGT 1 cut(s) 1507
SfaNI GCATC 4 cut(s) 163, 185, 1435, 1480
SfcI CTRYAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 614
SinI GGWCC 1 cut(s) 1088
SmiMI CAYNNNNRTG 1 cut(s) 1539
SmlI CTYRAG 2 cut(s) 575, 950
SmoI CTYRAG 2 cut(s) 575, 950
SseBI AGGCCT 1 cut(s) 1637
SspMI CTAG 1 cut(s) 1262
StuI AGGCCT 1 cut(s) 1637
StyD4I CCNGG 2 cut(s) 1507, 1616
StyI CCWWGG 1 cut(s) 1385
TaaI ACNGT 4 cut(s) 98, 689, 715, 1660
TaiI ACGT 1 cut(s) 467
TaqI TCGA 3 cut(s) 59, 92, 614
TfiI GAWTC 2 cut(s) 955, 1061
Tru1I TTAA 9 cut(s) 35, 576, 782, 845, 914, 951, 1032, 1334, 1575
Tru9I TTAA 9 cut(s) 35, 576, 782, 845, 914, 951, 1032, 1334, 1575
TscAI CASTG 3 cut(s) 19, 139, 300
TseFI GTSAC 2 cut(s) 604, 715
TseI GCWGC 2 cut(s) 370, 743
Tsp45I GTSAC 2 cut(s) 604, 715
TspDTI ATGAA 4 cut(s) 513, 524, 1089, 1245
TspRI CASTG 3 cut(s) 19, 139, 300
Vha464I CTTAAG 2 cut(s) 575, 950
VpaK11BI GGWCC 1 cut(s) 1088
XagI CCTNNNNNAGG 1 cut(s) 1265
XapI RAATTY 4 cut(s) 67, 753, 1397, 1693
XceI RCATGY 4 cut(s) 348, 605, 1147, 1366
XmiI GTMKAC 1 cut(s) 593
XmnI GAANNNNTTC 2 cut(s) 108, 403
XspI CTAG 1 cut(s) 1262
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.