Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0011105

Physical Location & Seq

Genomic Coordinates Forward (+)

1908066 .. 1914968

Gene Structure 6903 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g01500.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGCCATGGCAGTGGAAGCGGAGAGGACCGTGATGTGCCTTCTGACGGACCCAGATGACACTCCATTAGGACAGTTCATGTATCTTCCTCAACACACTACCACCCTCCAACTCCAACAAATCGTCAATCGCCTTCTTCAAAATGAGGAGAAGATGCCTTATACTTTCTACATAGAAAATAAGGAACTTAATGAGGCGCTGGGTGTATATGCGGAGAAGAACAAAATTTCTGTGGAGAAGGTACTCAAAATCGTGTATCAACCACAAGCTATTTTCCGAATTCGTCCTGTCAATCGTTGCTCGGCCACAATTGCTGGTCACTCAGAAGCTGTTCTTTCAGTTGCTTTTAGTCCTGATGGTCAACAGTTGGCGAGTGGTTCTGGTGATACCACTGTCCGAATATGGGACCTTAATACTCAAACACCTTTGTACACATGTTCAGGACATAAGAATTGGGTTCTTTGTATTGCTTGGTCTGCTGATGGAAAGTATCTTGTTAGTGGGAGCAAGTCTGGAGAACTTCAATGTTGGGATCCACAGACAGGAAAACCATCAGGCAATCCATTGATTGGCCATAAGAAATGGATTACTGGGATCTCTTGGGAACCAGTCCACCTTGCTGCTCCATGTAGACGATTTGTAAGTGCTGGGAAAGATGGTGATGCACGCATATGGGACATCACATTGAAGAAATGCGTTATGTCTCTCACCGGCCACACACTTGCAATAACCTGTGTAAAATGGGGTGGAGATGGTTTTATATATACAGGTTCCCAAGATTGTACAATCAAGGTCTGGGAGACTACCCAAGGGAAGCTGATTCGTGAATTAAAGGGTCATGGGCACTGGGTTAACACTCTTGCATTGAGTACTGAATATGTTCTTCGCACCGGAGCTTATGATCACACTGGCAAAAAGCAATATTCATCTCCTGAGGAAATGAAAAAGGCAGCTTTGGAAAGGTATAACAAAATGAAAGGCAAAGCTCCTGAAAGATTGGTTTCTGGATCAGATGATTTCACTATGTTTCTGTGGGAGCCTTTTGTCAGCAAACAACCCAAAGCTCGCATGACAGGTCATCAACAGCTTGTAAACCATGTCTACTTTTCACCTGATGGTCAATGGATAGCAAGTGCCTCATTTGATAGATCTGTCAGAATATGGAATGGTACAACTGGCAAGTTTTTTGGAGTTCTACGGGGTCATGTTGGTCCTGTTTACCAGATCAGCTGGTCCGCAGACAGCAGGCTACTTTTAAGTGGCAGCAAAGATTCAACTCTAAAGGTTTGGGATATCAGGACTATGAAACTGAAACAAGACCTCCCAGGTCATGCAGATGAGGTTTACGCTGTTGATTGGAGTCCAGATGGTGAAAAGGTTGCTTCTGGTGGTAAGGATAGAGTTTTGAAGTTATGGATGGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000027 GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005840 GO:0006996 GO:0007275 GO:0008150 GO:0009553 GO:0009987 GO:0016043 GO:0022607 GO:0022613 GO:0022618 GO:0031461 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0042254 GO:0042255 GO:0042273 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0065003 GO:0070013 GO:0070925 GO:0071826 GO:0071840 GO:0080008 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NLE WD40

Protein Analysis

475

Amino Acids

53.0

Weight (kDa)

8.84

Isoelectric Point (pI)

33.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NLE	PF08154	11 - 68	4.7e-10	NLE (NUC135) domain
Beta-prop_Aladin	PF25460	96 - 181	1.2e-07	Aladin seven-bladed propeller
WD40_Prp19	PF24814	98 - 145	2.9e-08	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	98 - 200	1.3e-21	WDR3 first beta-propeller domain
Beta-prop_HPS5	PF23756	99 - 182	6.7e-06	HPS5 beta-propeller
WD40	PF00400	99 - 136	2.8e-12	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	99 - 159	5.6e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	99 - 201	1.4e-22	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	100 - 178	3.5e-10	WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	100 - 233	2.4e-11	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	100 - 204	8e-22	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	100 - 227	2.7e-18	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	100 - 146	6.1e-09	WDHD1 first WD40 domain
WDR55	PF24796	102 - 184	1.5e-08	WDR55
Beta-prop_EML_2	PF23414	103 - 186	1.5e-14	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_1st	PF24780	103 - 168	4.1e-08	MABP1/WDR62 first WD40 domain
ANAPC4_WD40	PF12894	103 - 159	8.7e-06	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_EML	PF23409	106 - 319	3.7e-12	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_IFT140_1st	PF23383	107 - 193	1.2e-06	IFT140 first beta-propeller
Beta-prop_WDR19_1st	PF23389	109 - 202	5e-07	WDR19 first beta-propeller
Beta-prop_IFT122_1st	PF23381	111 - 253	5.4e-08	IFT122 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	121 - 180	4.8e-06	WDR90/POC16, second beta-propeller
WD40_Prp19	PF24814	139 - 302	8.5e-19	Prp19 WD40 domain
WD40	PF00400	141 - 178	8.7e-10	WD domain, G-beta repeat
WD40_Gbeta	PF25391	145 - 303	1.7e-09	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	147 - 203	1.2e-06	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	149 - 252	2.7e-17	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	189 - 302	1.1e-15	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	210 - 394	9e-10	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5	PF25175	210 - 303	6e-16	WDR5 beta-propeller domain
WDR55	PF24796	211 - 434	7.4e-16	WDR55
Beta-prop_WDR3_2nd	PF25172	213 - 302	5e-07	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	216 - 389	6.8e-08	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	217 - 302	5e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	222 - 290	2.7e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	231 - 266	4.7e-08	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	239 - 303	1.1e-06	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	321 - 412	1.1e-06	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	326 - 472	2.6e-21	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	330 - 472	3.2e-29	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	331 - 394	1.2e-06	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	331 - 439	8.4e-21	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	331 - 472	2.8e-15	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	332 - 472	8.2e-34	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	351 - 394	3e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	352 - 389	1.8e-10	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	352 - 437	8.9e-12	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	355 - 456	1.4e-10	WDR55
WD40_CDC20-Fz	PF24807	356 - 472	6e-20	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	357 - 471	4e-14	G protein beta WD-40 repeat protein
EIF3I	PF24805	357 - 436	8.4e-13	EIF3I
Beta-prop_WDR3_2nd	PF25172	357 - 472	3.1e-18	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	358 - 468	2.5e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3	PF25174	360 - 472	1.1e-27	THOC3 beta-propeller domain
WDR90_beta-prop_4th	PF23342	364 - 468	1.9e-08	WDR90, 4th beta-propeller
WD40	PF00400	394 - 431	1.2e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	397 - 471	4e-13	CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st	PF24817	398 - 472	7e-08	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	398 - 466	4.4e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_Aladin	PF25460	403 - 472	3.2e-08	Aladin seven-bladed propeller
WD40	PF00400	436 - 472	8.7e-09	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

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Orthologous Genes (Group: OG0011105)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52820
fragaria_vesca	FvH4_7g01500
malus_domestica	MD02G1305600.v1.1 MD09G1280600.v1.1 MD17G1287700.v1.1
prunus_persica	Prupe.2G020500_v2.0.a1
pyrus_communis	pycom02g25610 pycom17g27070
rosa_chinensis	RchiOBHm_Chr1g0317321
rosa_laevigata	RLG00000029568 RLG00000030606
rosa_multiflora	Rmu_sc0005092.1_g000002 Rmu_sc0013869.1_g000006
rosa_roxburghii	Rroxscaffold_4G00330490
rosa_rugosa	Rorug01G0015900
rosa_samantha	Rh1AG027300 Rh1BG023200 Rh1CG025300 Rh1DG045900
rosa_wichuraiana	Rw1G001910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	569
AccI	GTMKAC	2 cut(s)	631, 1101
AciI	CCGC	3 cut(s)	20, 212, 1236
AclWI	GGATC	4 cut(s)	527, 540, 602, 1015
AcoI	YGGCCR	4 cut(s)	3, 303, 571, 712
AcsI	RAATTY	2 cut(s)	225, 279
AfaI	GTAC	5 cut(s)	243, 431, 784, 871, 1171
AfiI	CCNNNNNNNGG	4 cut(s)	46, 403, 542, 569
AflIII	ACRYGT	1 cut(s)	434
AgsI	TTSAA	5 cut(s)	140, 524, 688, 1275, 1408
AjnI	CCWGG	1 cut(s)	1324
AluBI	AGCT	9 cut(s)	269, 329, 817, 896, 953, 986, 1064, 1087, 1230
AluI	AGCT	9 cut(s)	269, 329, 817, 896, 953, 986, 1064, 1087, 1230
Alw26I	GTCTC	2 cut(s)	708, 794
AlwI	GGATC	4 cut(s)	527, 540, 602, 1015
AlwNI	CAGNNNCTG	1 cut(s)	329
AoxI	GGCC	4 cut(s)	3, 303, 571, 712
ApeKI	GCWGC	3 cut(s)	620, 950, 1263
ApoI	RAATTY	2 cut(s)	225, 279
Asp700I	GAANNNNTTC	2 cut(s)	330, 879
AspLEI	GCGC	1 cut(s)	199
AspS9I	GGNCC	5 cut(s)	27, 49, 406, 1211, 1233
AsuHPI	GGTGA	5 cut(s)	395, 671, 700, 1101, 1382
AvaII	GGWCC	5 cut(s)	27, 49, 406, 1211, 1233
AxyI	CCTNAGG	1 cut(s)	933
BaeGI	GKGCMC	1 cut(s)	846
BaeI	ACNNNNGTAYC	2 cut(s)	378, 411
BalI	TGGCCA	2 cut(s)	5, 573
BamHI	GGATCC	1 cut(s)	532
BbvI	GCAGC	3 cut(s)	607, 962, 1275
BccI	CCATC	8 cut(s)	350, 476, 559, 650, 746, 1109, 1361, 1411
BciT130I	CCWGG	1 cut(s)	1326
BclI	TGATCA	1 cut(s)	901
BcoDI	GTCTC	2 cut(s)	708, 794
BfoI	RGCGCY	1 cut(s)	200
BglII	AGATCT	1 cut(s)	1148
BisI	GCNGC	3 cut(s)	621, 951, 1264
BlsI	GCNGC	3 cut(s)	622, 952, 1265
BmcAI	AGTACT	1 cut(s)	871
Bme1390I	CCNGG	1 cut(s)	1326
Bme18I	GGWCC	5 cut(s)	27, 49, 406, 1211, 1233
BmgT120I	GGNCC	5 cut(s)	27, 49, 406, 1211, 1233
BmiI	GGNNCC	6 cut(s)	51, 407, 534, 606, 772, 1038
BmrFI	CCNGG	1 cut(s)	1326
BmrI	ACTGGG	2 cut(s)	600, 856
BmsI	GCATC	2 cut(s)	144, 652
BmuI	ACTGGG	2 cut(s)	600, 856
BpmI	CTGGAG	1 cut(s)	534
BsaJI	CCNNGG	3 cut(s)	6, 808, 1324
BsaWI	WCCGGW	1 cut(s)	890
BsaXI	ACNNNNNCTCC	2 cut(s)	1305, 1335
Bsc4I	CCNNNNNNNGG	4 cut(s)	46, 403, 542, 569
Bse118I	RCCGGY	1 cut(s)	710
Bse1I	ACTGG	5 cut(s)	595, 608, 851, 913, 1180
Bse21I	CCTNAGG	1 cut(s)	933
BseBI	CCWGG	1 cut(s)	1326
BseDI	CCNNGG	3 cut(s)	6, 808, 1324
BseGI	GGATG	1 cut(s)	1422
BseLI	CCNNNNNNNGG	4 cut(s)	46, 403, 542, 569
BseMII	CTCAG	2 cut(s)	336, 924
BseNI	ACTGG	5 cut(s)	595, 608, 851, 913, 1180
BseRI	GAGGAG	1 cut(s)	161
BseSI	GKGCMC	1 cut(s)	846
BseXI	GCAGC	3 cut(s)	607, 962, 1275
BseYI	CCCAGC	2 cut(s)	199, 647
BshFI	GGCC	4 cut(s)	5, 305, 573, 714
BsiSI	CCGG	2 cut(s)	711, 891
BslFI	GGGAC	2 cut(s)	419, 689
BslI	CCNNNNNNNGG	4 cut(s)	46, 403, 542, 569
BsmAI	GTCTC	2 cut(s)	708, 794
BsmFI	GGGAC	2 cut(s)	419, 689
BsnI	GGCC	4 cut(s)	5, 305, 573, 714
Bsp1286I	GDGCHC	1 cut(s)	846
Bsp1407I	TGTACA	2 cut(s)	429, 782
Bsp143I	GATC	6 cut(s)	532, 594, 901, 1007, 1148, 1224
Bsp19I	CCATGG	1 cut(s)	6
BspACI	CCGC	3 cut(s)	20, 212, 1236
BspANI	GGCC	4 cut(s)	5, 305, 573, 714
BspCNI	CTCAG	2 cut(s)	335, 925
BspLI	GGNNCC	6 cut(s)	51, 407, 534, 606, 772, 1038
BspPI	GGATC	4 cut(s)	527, 540, 602, 1015
BsrFI	RCCGGY	1 cut(s)	710
BsrGI	TGTACA	2 cut(s)	429, 782
BsrI	ACTGG	5 cut(s)	595, 608, 851, 913, 1180
BssAI	RCCGGY	1 cut(s)	710
BssECI	CCNNGG	3 cut(s)	6, 808, 1324
BssMI	GATC	6 cut(s)	532, 594, 901, 1007, 1148, 1224
BssT1I	CCWWGG	2 cut(s)	6, 808
Bst2UI	CCWGG	1 cut(s)	1326
Bst4CI	ACNGT	4 cut(s)	31, 75, 366, 394
BstAUI	TGTACA	2 cut(s)	429, 782
BstC8I	GCNNGC	3 cut(s)	667, 1066, 1247
BstDEI	CTNAG	2 cut(s)	322, 933
BstDSI	CCRYGG	1 cut(s)	6
BstF5I	GGATG	1 cut(s)	1422
BstH2I	RGCGCY	1 cut(s)	200
BstHHI	GCGC	1 cut(s)	199
BstKTI	GATC	6 cut(s)	535, 597, 904, 1010, 1151, 1227
BstMAI	GTCTC	2 cut(s)	708, 794
BstMBI	GATC	6 cut(s)	532, 594, 901, 1007, 1148, 1224
BstMWI	GCNNNNNNNGC	3 cut(s)	17, 311, 476
BstNI	CCWGG	1 cut(s)	1326
BstNSI	RCATGY	1 cut(s)	438
BstSCI	CCNGG	1 cut(s)	1324
BstSLI	GKGCMC	1 cut(s)	846
BstV1I	GCAGC	3 cut(s)	607, 962, 1275
BstX2I	RGATCY	3 cut(s)	532, 594, 1148
BstXI	CCANNNNNNTGG	1 cut(s)	13
BstYI	RGATCY	3 cut(s)	532, 594, 1148
Bsu36I	CCTNAGG	1 cut(s)	933
BsuRI	GGCC	4 cut(s)	5, 305, 573, 714
BtgI	CCRYGG	1 cut(s)	6
BtsCI	GGATG	1 cut(s)	1422
BtsI	GCAGTG	1 cut(s)	18
BtsIMutI	CAGTG	4 cut(s)	18, 390, 844, 906
Cac8I	GCNNGC	3 cut(s)	667, 1066, 1247
CaiI	CAGNNNCTG	1 cut(s)	329
CfoI	GCGC	1 cut(s)	199
Cfr10I	RCCGGY	1 cut(s)	710
Cfr13I	GGNCC	5 cut(s)	27, 49, 406, 1211, 1233
Csp6I	GTAC	5 cut(s)	242, 430, 783, 870, 1170
CviAII	CATG	9 cut(s)	7, 79, 435, 627, 839, 1069, 1097, 1205, 1331
CviQI	GTAC	5 cut(s)	242, 430, 783, 870, 1170
DdeI	CTNAG	2 cut(s)	322, 933
DpnI	GATC	6 cut(s)	534, 596, 903, 1009, 1150, 1226
DpnII	GATC	6 cut(s)	532, 594, 901, 1007, 1148, 1224
EaeI	YGGCCR	4 cut(s)	3, 303, 571, 712
Eco130I	CCWWGG	2 cut(s)	6, 808
Eco32I	GATATC	1 cut(s)	1294
Eco47I	GGWCC	5 cut(s)	27, 49, 406, 1211, 1233
Eco81I	CCTNAGG	1 cut(s)	933
EcoO109I	RGGNCCY	1 cut(s)	406
EcoRI	GAATTC	1 cut(s)	279
EcoRII	CCWGG	1 cut(s)	1324
EcoRV	GATATC	1 cut(s)	1294
EcoT14I	CCWWGG	2 cut(s)	6, 808
ErhI	CCWWGG	2 cut(s)	6, 808
FaeI	CATG	9 cut(s)	10, 82, 438, 630, 842, 1072, 1100, 1208, 1334
FalI	AAGNNNNNCTT	2 cut(s)	318, 350
FaqI	GGGAC	2 cut(s)	419, 689
FatI	CATG	9 cut(s)	6, 78, 434, 626, 838, 1068, 1096, 1204, 1330
FauNDI	CATATG	1 cut(s)	671
FbaI	TGATCA	1 cut(s)	901
FblI	GTMKAC	2 cut(s)	631, 1101
Fnu4HI	GCNGC	3 cut(s)	621, 951, 1264
Fsp4HI	GCNGC	3 cut(s)	621, 951, 1264
GlaI	GCGC	1 cut(s)	198
GluI	GCNGC	3 cut(s)	621, 951, 1264
GsaI	CCCAGC	2 cut(s)	203, 651
GsuI	CTGGAG	1 cut(s)	534
HaeII	RGCGCY	1 cut(s)	200
HaeIII	GGCC	4 cut(s)	5, 305, 573, 714
HapII	CCGG	2 cut(s)	711, 891
HhaI	GCGC	1 cut(s)	199
Hin1II	CATG	9 cut(s)	10, 82, 438, 630, 842, 1072, 1100, 1208, 1334
Hin6I	GCGC	1 cut(s)	197
HinP1I	GCGC	1 cut(s)	197
HincII	GTYRAC	2 cut(s)	362, 853
HindII	GTYRAC	2 cut(s)	362, 853
HinfI	GANTC	3 cut(s)	820, 1271, 1360
HpaI	GTTAAC	1 cut(s)	853
HpaII	CCGG	2 cut(s)	711, 891
HphI	GGTGA	5 cut(s)	395, 671, 700, 1101, 1382
Hpy166II	GTNNAC	9 cut(s)	362, 432, 613, 632, 853, 1093, 1102, 1219, 1345
Hpy188I	TCNGA	6 cut(s)	45, 278, 325, 398, 1012, 1157
Hpy188III	TCNNGA	9 cut(s)	353, 441, 513, 824, 932, 989, 1005, 1297, 1364
Hpy8I	GTNNAC	9 cut(s)	362, 432, 613, 632, 853, 1093, 1102, 1219, 1345
HpyAV	CCTTC	3 cut(s)	50, 143, 232
HpyCH4III	ACNGT	4 cut(s)	31, 75, 366, 394
HpyCH4V	TGCA	4 cut(s)	665, 725, 863, 1334
HpyF10VI	GCNNNNNNNGC	3 cut(s)	17, 311, 476
HpyF3I	CTNAG	2 cut(s)	322, 933
Hsp92II	CATG	9 cut(s)	10, 82, 438, 630, 842, 1072, 1100, 1208, 1334
HspAI	GCGC	1 cut(s)	197
Ksp22I	TGATCA	1 cut(s)	901
KspAI	GTTAAC	1 cut(s)	853
Kzo9I	GATC	6 cut(s)	532, 594, 901, 1007, 1148, 1224
LmnI	GCTCC	5 cut(s)	504, 628, 893, 991, 1036
Lsp1109I	GCAGC	3 cut(s)	607, 962, 1275
LweI	GCATC	2 cut(s)	144, 652
MaeIII	GTNAC	1 cut(s)	317
MalI	GATC	6 cut(s)	534, 596, 903, 1009, 1150, 1226
MboI	GATC	6 cut(s)	532, 594, 901, 1007, 1148, 1224
MboII	GAAGA	6 cut(s)	77, 128, 163, 229, 700, 875
MfeI	CAATTG	1 cut(s)	309
MflI	RGATCY	3 cut(s)	532, 594, 1148
MhlI	GDGCHC	1 cut(s)	846
MlsI	TGGCCA	2 cut(s)	5, 573
MluCI	AATT	5 cut(s)	225, 279, 309, 451, 827
MluNI	TGGCCA	2 cut(s)	5, 573
MlyI	GAGTC	1 cut(s)	1369
MmeI	TCCRAC	2 cut(s)	133, 139
MnlI	CCTC	9 cut(s)	18, 99, 116, 139, 187, 928, 1147, 1331, 1333
Mox20I	TGGCCA	2 cut(s)	5, 573
MroXI	GAANNNNTTC	2 cut(s)	330, 879
MscI	TGGCCA	2 cut(s)	5, 573
MseI	TTAA	6 cut(s)	189, 411, 830, 852, 1256, 1423
MslI	CAYNNNNRTG	3 cut(s)	11, 670, 1335
Msp20I	TGGCCA	2 cut(s)	5, 573
MspA1I	CMGCKG	1 cut(s)	1230
MspI	CCGG	2 cut(s)	711, 891
MspR9I	CCNGG	1 cut(s)	1326
MunI	CAATTG	1 cut(s)	309
MvaI	CCWGG	1 cut(s)	1326
MwoI	GCNNNNNNNGC	3 cut(s)	17, 311, 476
NcoI	CCATGG	1 cut(s)	6
NdeI	CATATG	1 cut(s)	671
NdeII	GATC	6 cut(s)	532, 594, 901, 1007, 1148, 1224
NlaIII	CATG	9 cut(s)	10, 82, 438, 630, 842, 1072, 1100, 1208, 1334
NlaIV	GGNNCC	6 cut(s)	51, 407, 534, 606, 772, 1038
NmeAIII	GCCGAG	1 cut(s)	281
NmuCI	GTSAC	1 cut(s)	317
NspI	RCATGY	1 cut(s)	438
PciI	ACATGT	1 cut(s)	434
PdmI	GAANNNNTTC	2 cut(s)	330, 879
PfeI	GAWTC	2 cut(s)	820, 1271
PflMI	CCANNNNNTGG	1 cut(s)	569
PkrI	GCNGC	3 cut(s)	622, 952, 1265
PleI	GAGTC	1 cut(s)	1368
PpsI	GAGTC	1 cut(s)	1368
PpuMI	RGGWCCY	1 cut(s)	406
PscI	ACATGT	1 cut(s)	434
Psp5II	RGGWCCY	1 cut(s)	406
Psp6I	CCWGG	1 cut(s)	1324
PspFI	CCCAGC	2 cut(s)	199, 647
PspGI	CCWGG	1 cut(s)	1324
PspN4I	GGNNCC	6 cut(s)	51, 407, 534, 606, 772, 1038
PspPI	GGNCC	5 cut(s)	27, 49, 406, 1211, 1233
PspPPI	RGGWCCY	1 cut(s)	406
PstNI	CAGNNNCTG	1 cut(s)	329
PsuI	RGATCY	3 cut(s)	532, 594, 1148
PvuII	CAGCTG	1 cut(s)	1230
RsaI	GTAC	5 cut(s)	243, 431, 784, 871, 1171
RsaNI	GTAC	5 cut(s)	242, 430, 783, 870, 1170
RseI	CAYNNNNRTG	3 cut(s)	11, 670, 1335
SaqAI	TTAA	6 cut(s)	189, 411, 830, 852, 1256, 1423
SatI	GCNGC	3 cut(s)	621, 951, 1264
Sau3AI	GATC	6 cut(s)	532, 594, 901, 1007, 1148, 1224
Sau96I	GGNCC	5 cut(s)	27, 49, 406, 1211, 1233
ScaI	AGTACT	1 cut(s)	871
SchI	GAGTC	1 cut(s)	1369
ScrFI	CCNGG	1 cut(s)	1326
SduI	GDGCHC	1 cut(s)	846
SfaNI	GCATC	2 cut(s)	144, 652
SinI	GGWCC	5 cut(s)	27, 49, 406, 1211, 1233
SmiMI	CAYNNNNRTG	3 cut(s)	11, 670, 1335
Sse9I	AATT	5 cut(s)	225, 279, 309, 451, 827
SsiI	CCGC	3 cut(s)	20, 212, 1236
SspI	AATATT	1 cut(s)	923
StyD4I	CCNGG	1 cut(s)	1324
StyI	CCWWGG	2 cut(s)	6, 808
TaaI	ACNGT	4 cut(s)	31, 75, 366, 394
TasI	AATT	5 cut(s)	225, 279, 309, 451, 827
TatI	WGTACW	3 cut(s)	429, 782, 869
TfiI	GAWTC	2 cut(s)	820, 1271
Tru1I	TTAA	6 cut(s)	189, 411, 830, 852, 1256, 1423
Tru9I	TTAA	6 cut(s)	189, 411, 830, 852, 1256, 1423
TscAI	CASTG	4 cut(s)	18, 397, 851, 913
TseFI	GTSAC	1 cut(s)	317
TseI	GCWGC	3 cut(s)	620, 950, 1263
Tsp45I	GTSAC	1 cut(s)	317
TspDTI	ATGAA	5 cut(s)	67, 915, 956, 989, 1319
TspGWI	ACGGA	1 cut(s)	62
TspRI	CASTG	4 cut(s)	18, 397, 851, 913
Van91I	CCANNNNNTGG	1 cut(s)	569
VpaK11BI	GGWCC	5 cut(s)	27, 49, 406, 1211, 1233
XapI	RAATTY	2 cut(s)	225, 279
XceI	RCATGY	1 cut(s)	438
XmiI	GTMKAC	2 cut(s)	631, 1101
XmnI	GAANNNNTTC	2 cut(s)	330, 879
ZrmI	AGTACT	1 cut(s)	871

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.