Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011105

Physical Location & Seq

Genomic Coordinates Reverse (-)

35867186 .. 35874420

Gene Structure 7235 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1305600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1434 bp

ATGACAATGGAAGTGGAAGGAGGAGAGGGGCAGGGGAAGACTATGTTGTGCCTTCTGACGGACCCAGATGGAAATCCATTAGGAACTCCGGTCAACTTTCCTCTAAACACTGAACCGCAACACCTTCAACAAATCGTTAATCAGCTTCGTCAAAATGAGGAGAGGCTACCGTATGCTTTCTATATAGCAGATAAGGAACTTGTTGAGTCACTTGGAAAGTTTGCAGAGAAGAACAAAATTTCTGTGGAGAAGGTGCTTCATATAGTTTATCAACCACAAGCTGTTTTCCGAATTCGGCCTGTTCATCGTTGCTCAGCAACAATTGCTGGTCACACAGAAGCTGTACTTTCAGTTGCTTTTAGTCCTGATGGCCAACAGTTGGCAAGTGGTTCTGGTGATACCACTGTCCGACTATGGGACCTTAATACTCAGACGCCGCTGTTCACATGTACAGGACATAAGAATTGGGTCCTTGTTATTGCATGGTCACCTGATGGTAAGTATCTTGTTAGTGGCAGCAAGGCTGGAGAACTTCAATGTTGGGATCCACGAACAGGGAAGCCATCAGGCAATCCACTTATTGGCCACAAGAAATGGATTACTGGAATCTCTTGGGAACCAGTCCACCTAAGTGCTCTGTGTCGACGCTTTGTAAGTGCCGGCAAAGATGGTGATGCACGTATATGGGATGTTACGTTGAGGAAATCTGTTATATGTCTTAGTGGCCACACACTTGCAGTAACATGTGTAAAATGGGGTGGAGATGGTTTCATATATACAGGCTCCCAGGATTGTACTATCAAAGTCTGGGAGACCACACAAGGAAAACTAATTCGTGAACTGAAGGGTCATGGTCATTGGGTTAACTCTCTTACATTGAGTACTGAATATGTTCTTCGTACGGGAGCCTTTGATCACACTGGCAAGCAATATTCATCTCCAGAGGAAATGAAGCAGGCAGCTTTGGAAAGATATAACAAAATGAAAGGCGATTCCCCTGAAAGATTGGTTTCTGGATCTGATGATTTCACAATGTTTTTATGGGAACCTTTTCTCAAGAAACAACCTAAAACTCGCATGACGGGTCACCAACAGCCTATAAACCACGTCTGCTTTTCACCTGATGGGCAATGGATAGCAAGTGCCTCATTTGATAGGTCTGTCAAGTTATGGAATGGTACTACTGGAAAATTTGTTGCAGCTTTCCGGGGCCACGTTGGCCCTGTCTACCAGATCAGCTGGTCAGCAGACAGCAGGCTACTTGTTAGTGGCAGCAAAGATTCCACTCTTAAGGTTTGGGATATCAAAGCACGGGCGCTAAAACTAGATCTTCCTGGCCATGCAGATGAGGTTTTTGCTGTTGATTGGAGTCCAGATGGTCAGAAGGTTGCTTCTGGTGGTAAAGATAGAGTTCTGAAGTTATGGATGGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000027 GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005840 GO:0006996 GO:0007275 GO:0008150 GO:0009553 GO:0009987 GO:0016043 GO:0022607 GO:0022613 GO:0022618 GO:0031461 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0042254 GO:0042255 GO:0042273 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0065003 GO:0070013 GO:0070925 GO:0071826 GO:0071840 GO:0080008 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NLE WD40

Protein Analysis

478

Amino Acids

52.92

Weight (kDa)

8.7

Isoelectric Point (pI)

32.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NLE	PF08154	18 - 74	7.8e-11	NLE (NUC135) domain
Beta-prop_Aladin	PF25460	99 - 184	4.9e-07	Aladin seven-bladed propeller
WD40_CDC20-Fz	PF24807	102 - 165	1.6e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	102 - 203	1.4e-20	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	103 - 231	6.2e-18	TEP-1 second beta-propeller
WD40	PF00400	103 - 140	3.6e-12	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	104 - 207	8.5e-22	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	104 - 182	2.9e-09	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	104 - 205	2.6e-21	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	105 - 147	5.3e-08	WDHD1 first WD40 domain
WD40_Gbeta	PF25391	105 - 271	2.2e-18	G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1	PF24105	106 - 182	9e-11	CAF1B/HIR1 beta-propeller domain
WDR55	PF24796	106 - 188	8.4e-09	WDR55
Beta-prop_EML_2	PF23414	107 - 190	1.7e-13	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_1st	PF24780	108 - 172	7.5e-07	MABP1/WDR62 first WD40 domain
Beta-prop_EML	PF23409	110 - 310	9e-10	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	111 - 187	1.6e-13	Prp19 WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	112 - 182	6.6e-07	WDR90/POC16, second beta-propeller
Beta-prop_WDR19_1st	PF23389	113 - 210	4.3e-07	WDR19 first beta-propeller
Beta-prop_IFT140_1st	PF23383	113 - 196	7.2e-06	IFT140 first beta-propeller
Beta-prop_IFT122_1st	PF23381	115 - 256	3e-06	IFT122 first beta-propeller
WD40	PF00400	145 - 182	3.9e-08	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	151 - 208	5.9e-07	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	153 - 256	6.4e-15	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	167 - 295	1.4e-13	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	193 - 310	2.2e-15	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	195 - 276	1.7e-06	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	196 - 289	1.7e-11	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	208 - 306	1.2e-15	WDR5 beta-propeller domain
WD40_Prp19	PF24814	215 - 294	6.3e-11	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	215 - 302	6.8e-07	TEP-1 second beta-propeller
WD40	PF00400	235 - 270	9e-07	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	327 - 474	4.4e-17	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	331 - 475	3.1e-21	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	331 - 475	2.9e-16	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	332 - 401	1.9e-08	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	333 - 397	6.4e-11	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	334 - 438	2e-20	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	335 - 475	6.4e-34	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	355 - 437	1.7e-11	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	356 - 392	6.3e-12	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	357 - 475	2.1e-20	CDC20/Fizzy WD40 domain
WDR55	PF24796	357 - 463	2.1e-11	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	357 - 403	3.4e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	358 - 476	1.4e-06	WDR36/Utp21 first beta-propeller
EIF3I	PF24805	360 - 437	3.6e-13	EIF3I
Beta-prop_WDR3_2nd	PF25172	360 - 475	5.6e-18	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	363 - 475	4.8e-26	THOC3 beta-propeller domain
WDR90_beta-prop_4th	PF23342	367 - 471	5.6e-11	WDR90, 4th beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	367 - 434	3.2e-07	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st	PF25173	373 - 475	3.7e-23	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	381 - 474	1.1e-12	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	395 - 469	8.4e-09	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	396 - 434	3.9e-10	WD domain, G-beta repeat
Beta-prop_Aladin	PF25460	407 - 475	1.2e-08	Aladin seven-bladed propeller
WD40_WDHD1_1st	PF24817	408 - 475	1.6e-07	WDHD1 first WD40 domain
WD40	PF00400	444 - 475	4.3e-08	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011105)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52820
fragaria_vesca	FvH4_7g01500
malus_domestica	MD02G1305600.v1.1 MD09G1280600.v1.1 MD17G1287700.v1.1
prunus_persica	Prupe.2G020500_v2.0.a1
pyrus_communis	pycom02g25610 pycom17g27070
rosa_chinensis	RchiOBHm_Chr1g0317321
rosa_laevigata	RLG00000029568 RLG00000030606
rosa_multiflora	Rmu_sc0005092.1_g000002 Rmu_sc0013869.1_g000006
rosa_roxburghii	Rroxscaffold_4G00330490
rosa_rugosa	Rorug01G0015900
rosa_samantha	Rh1AG027300 Rh1BG023200 Rh1CG025300 Rh1DG045900
rosa_wichuraiana	Rw1G001910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	379, 581
AccI	GTMKAC	2 cut(s)	643, 1227
AciI	CCGC	2 cut(s)	116, 437
AclWI	GGATC	3 cut(s)	539, 552, 1024
AcoI	YGGCCR	4 cut(s)	370, 583, 724, 1336
AcsI	RAATTY	3 cut(s)	237, 291, 1190
AcuI	CTGAAG	1 cut(s)	863
AcyI	GRCGYC	1 cut(s)	434
AfaI	GTAC	6 cut(s)	345, 451, 796, 883, 901, 1180
AfiI	CCNNNNNNNGG	5 cut(s)	58, 379, 415, 554, 581
AflII	CTTAAG	1 cut(s)	1289
AflIII	ACRYGT	2 cut(s)	446, 743
AgsI	TTSAA	2 cut(s)	128, 536
AjiI	CACGTC	1 cut(s)	1108
AjnI	CCWGG	2 cut(s)	786, 1333
AleI	CACNNNNGTG	1 cut(s)	630
AluBI	AGCT	6 cut(s)	145, 281, 341, 962, 1202, 1239
AluI	AGCT	6 cut(s)	145, 281, 341, 962, 1202, 1239
Alw21I	GWGCWC	1 cut(s)	637
Alw26I	GTCTC	1 cut(s)	806
AlwI	GGATC	3 cut(s)	539, 552, 1024
AlwNI	CAGNNNCTG	1 cut(s)	341
AoxI	GGCC	7 cut(s)	296, 370, 583, 724, 1210, 1219, 1336
ApeKI	GCWGC	4 cut(s)	516, 959, 1199, 1272
ApoI	RAATTY	3 cut(s)	237, 291, 1190
Asp700I	GAANNNNTTC	2 cut(s)	891, 1050
AspLEI	GCGC	1 cut(s)	1318
AspS9I	GGNCC	5 cut(s)	61, 418, 469, 1210, 1220
AsuC2I	CCSGG	1 cut(s)	1208
AsuHPI	GGTGA	5 cut(s)	407, 480, 683, 1079, 1110
AvaII	GGWCC	3 cut(s)	61, 418, 469
BaeI	ACNNNNGTAYC	2 cut(s)	390, 423
BalI	TGGCCA	4 cut(s)	372, 585, 726, 1338
BamHI	GGATCC	1 cut(s)	544
BbsI	GAAGAC	1 cut(s)	44
Bbv12I	GWGCWC	1 cut(s)	637
BbvI	GCAGC	4 cut(s)	528, 971, 1211, 1284
BccI	CCATC	9 cut(s)	62, 362, 488, 571, 662, 758, 1118, 1370, 1420
BciT130I	CCWGG	2 cut(s)	788, 1335
BclI	TGATCA	1 cut(s)	913
BcnI	CCSGG	1 cut(s)	1208
BcoDI	GTCTC	1 cut(s)	806
BfaI	CTAG	2 cut(s)	1325, 1432
BfoI	RGCGCY	1 cut(s)	1319
BfrI	CTTAAG	1 cut(s)	1289
BglI	GCCNNNNNGGC	1 cut(s)	1218
BglII	AGATCT	1 cut(s)	1327
BisI	GCNGC	5 cut(s)	437, 517, 960, 1200, 1273
BlpI	GCTNAGC	1 cut(s)	313
BlsI	GCNGC	5 cut(s)	438, 518, 961, 1201, 1274
BmcAI	AGTACT	1 cut(s)	883
Bme1390I	CCNGG	3 cut(s)	788, 1208, 1335
Bme18I	GGWCC	3 cut(s)	61, 418, 469
BmgBI	CACGTC	1 cut(s)	1108
BmgT120I	GGNCC	5 cut(s)	61, 418, 469, 1210, 1220
BmiI	GGNNCC	9 cut(s)	63, 419, 470, 546, 618, 784, 907, 1047, 1211
BmrFI	CCNGG	3 cut(s)	788, 1208, 1335
BmsI	GCATC	1 cut(s)	664
BpiI	GAAGAC	1 cut(s)	44
BpmI	CTGGAG	2 cut(s)	546, 924
Bpu1102I	GCTNAGC	1 cut(s)	313
BpuEI	CTTGAG	1 cut(s)	1040
BpuMI	CCSGG	1 cut(s)	1208
BsaAI	YACGTR	1 cut(s)	680
BsaBI	GATNNNNATC	1 cut(s)	72
BsaHI	GRCGYC	1 cut(s)	434
BsaI	GGTCTC	1 cut(s)	806
BsaJI	CCNNGG	2 cut(s)	786, 1207
BsaWI	WCCGGW	1 cut(s)	88
Bsc4I	CCNNNNNNNGG	5 cut(s)	58, 379, 415, 554, 581
Bse118I	RCCGGY	1 cut(s)	659
Bse1I	ACTGG	4 cut(s)	607, 620, 925, 1189
Bse3DI	GCAATG	1 cut(s)	1136
Bse8I	GATNNNNATC	1 cut(s)	72
BseBI	CCWGG	2 cut(s)	788, 1335
BseDI	CCNNGG	2 cut(s)	786, 1207
BseGI	GGATG	2 cut(s)	694, 1431
BseJI	GATNNNNATC	1 cut(s)	72
BseLI	CCNNNNNNNGG	5 cut(s)	58, 379, 415, 554, 581
BseMI	GCAATG	1 cut(s)	1136
BseMII	CTCAG	2 cut(s)	327, 443
BseNI	ACTGG	4 cut(s)	607, 620, 925, 1189
BseRI	GAGGAG	2 cut(s)	36, 173
BseXI	GCAGC	4 cut(s)	528, 971, 1211, 1284
BshFI	GGCC	7 cut(s)	298, 372, 585, 726, 1212, 1221, 1338
BsiHKAI	GWGCWC	1 cut(s)	637
BsiSI	CCGG	3 cut(s)	89, 660, 1207
BsiWI	CGTACG	1 cut(s)	899
BslFI	GGGAC	1 cut(s)	431
BslI	CCNNNNNNNGG	5 cut(s)	58, 379, 415, 554, 581
BsmAI	GTCTC	1 cut(s)	806
BsmFI	GGGAC	1 cut(s)	431
BsnI	GGCC	7 cut(s)	298, 372, 585, 726, 1212, 1221, 1338
Bso31I	GGTCTC	1 cut(s)	806
Bsp1286I	GDGCHC	1 cut(s)	637
Bsp1407I	TGTACA	1 cut(s)	449
Bsp143I	GATC	5 cut(s)	544, 913, 1016, 1233, 1327
Bsp1720I	GCTNAGC	1 cut(s)	313
BspACI	CCGC	2 cut(s)	116, 437
BspANI	GGCC	7 cut(s)	298, 372, 585, 726, 1212, 1221, 1338
BspCNI	CTCAG	2 cut(s)	326, 442
BspLI	GGNNCC	9 cut(s)	63, 419, 470, 546, 618, 784, 907, 1047, 1211
BspPI	GGATC	3 cut(s)	539, 552, 1024
BspTI	CTTAAG	1 cut(s)	1289
BspTNI	GGTCTC	1 cut(s)	806
BsrDI	GCAATG	1 cut(s)	1136
BsrFI	RCCGGY	1 cut(s)	659
BsrGI	TGTACA	1 cut(s)	449
BsrI	ACTGG	4 cut(s)	607, 620, 925, 1189
BssAI	RCCGGY	1 cut(s)	659
BssECI	CCNNGG	2 cut(s)	786, 1207
BssMI	GATC	5 cut(s)	544, 913, 1016, 1233, 1327
BssNI	GRCGYC	1 cut(s)	434
Bst2UI	CCWGG	2 cut(s)	788, 1335
Bst4CI	ACNGT	3 cut(s)	171, 378, 406
BstACI	GRCGYC	1 cut(s)	434
BstAFI	CTTAAG	1 cut(s)	1289
BstAPI	GCANNNNNTGC	1 cut(s)	323
BstAUI	TGTACA	1 cut(s)	449
BstBAI	YACGTR	1 cut(s)	680
BstC8I	GCNNGC	4 cut(s)	661, 926, 957, 1256
BstDEI	CTNAG	4 cut(s)	313, 429, 629, 719
BstEII	GGTNACC	2 cut(s)	486, 1085
BstF5I	GGATG	2 cut(s)	694, 1431
BstH2I	RGCGCY	1 cut(s)	1319
BstHHI	GCGC	1 cut(s)	1318
BstKTI	GATC	5 cut(s)	547, 916, 1019, 1236, 1330
BstMAI	GTCTC	1 cut(s)	806
BstMBI	GATC	5 cut(s)	544, 913, 1016, 1233, 1327
BstMWI	GCNNNNNNNGC	2 cut(s)	323, 1218
BstNI	CCWGG	2 cut(s)	788, 1335
BstNSI	RCATGY	2 cut(s)	450, 747
BstPI	GGTNACC	2 cut(s)	486, 1085
BstSCI	CCNGG	3 cut(s)	786, 1206, 1333
BstV1I	GCAGC	4 cut(s)	528, 971, 1211, 1284
BstV2I	GAAGAC	1 cut(s)	44
BstX2I	RGATCY	3 cut(s)	544, 1016, 1327
BstYI	RGATCY	3 cut(s)	544, 1016, 1327
BsuRI	GGCC	7 cut(s)	298, 372, 585, 726, 1212, 1221, 1338
BtrI	CACGTC	1 cut(s)	1108
BtsCI	GGATG	2 cut(s)	694, 1431
BtsIMutI	CAGTG	3 cut(s)	108, 402, 918
Cac8I	GCNNGC	4 cut(s)	661, 926, 957, 1256
CaiI	CAGNNNCTG	1 cut(s)	341
CfoI	GCGC	1 cut(s)	1318
Cfr10I	RCCGGY	1 cut(s)	659
Cfr13I	GGNCC	5 cut(s)	61, 418, 469, 1210, 1220
CseI	GACGC	2 cut(s)	442, 654
Csp6I	GTAC	6 cut(s)	344, 450, 795, 882, 900, 1179
CspCI	CAANNNNNGTGG	1 cut(s)	29
CviAII	CATG	6 cut(s)	447, 483, 744, 851, 1078, 1340
CviQI	GTAC	6 cut(s)	344, 450, 795, 882, 900, 1179
DdeI	CTNAG	4 cut(s)	313, 429, 629, 719
DpnI	GATC	5 cut(s)	546, 915, 1018, 1235, 1329
DpnII	GATC	5 cut(s)	544, 913, 1016, 1233, 1327
EaeI	YGGCCR	4 cut(s)	370, 583, 724, 1336
Eco31I	GGTCTC	1 cut(s)	806
Eco32I	GATATC	1 cut(s)	1303
Eco47I	GGWCC	3 cut(s)	61, 418, 469
Eco57I	CTGAAG	1 cut(s)	863
Eco91I	GGTNACC	2 cut(s)	486, 1085
EcoO109I	RGGNCCY	2 cut(s)	418, 469
EcoO65I	GGTNACC	2 cut(s)	486, 1085
EcoRI	GAATTC	1 cut(s)	291
EcoRII	CCWGG	2 cut(s)	786, 1333
EcoRV	GATATC	1 cut(s)	1303
FaeI	CATG	6 cut(s)	450, 486, 747, 854, 1081, 1343
FalI	AAGNNNNNCTT	2 cut(s)	330, 362
FaqI	GGGAC	1 cut(s)	431
FatI	CATG	6 cut(s)	446, 482, 743, 850, 1077, 1339
FbaI	TGATCA	1 cut(s)	913
FblI	GTMKAC	2 cut(s)	643, 1227
Fnu4HI	GCNGC	5 cut(s)	437, 517, 960, 1200, 1273
FokI	GGATG	1 cut(s)	701
Fsp4HI	GCNGC	5 cut(s)	437, 517, 960, 1200, 1273
FspBI	CTAG	2 cut(s)	1325, 1432
GlaI	GCGC	1 cut(s)	1317
GluI	GCNGC	5 cut(s)	437, 517, 960, 1200, 1273
GsuI	CTGGAG	2 cut(s)	546, 924
HaeII	RGCGCY	1 cut(s)	1319
HaeIII	GGCC	7 cut(s)	298, 372, 585, 726, 1212, 1221, 1338
HapII	CCGG	3 cut(s)	89, 660, 1207
HgaI	GACGC	2 cut(s)	442, 654
HhaI	GCGC	1 cut(s)	1318
Hin1I	GRCGYC	1 cut(s)	434
Hin1II	CATG	6 cut(s)	450, 486, 747, 854, 1081, 1343
Hin6I	GCGC	1 cut(s)	1316
HinP1I	GCGC	1 cut(s)	1316
HincII	GTYRAC	3 cut(s)	94, 644, 865
HindII	GTYRAC	3 cut(s)	94, 644, 865
HinfI	GANTC	5 cut(s)	206, 606, 992, 1280, 1369
HpaI	GTTAAC	1 cut(s)	865
HpaII	CCGG	3 cut(s)	89, 660, 1207
HphI	GGTGA	5 cut(s)	407, 480, 683, 1079, 1110
Hpy166II	GTNNAC	7 cut(s)	94, 444, 625, 644, 839, 865, 1228
Hpy188I	TCNGA	7 cut(s)	57, 290, 410, 432, 1021, 1383, 1416
Hpy188III	TCNNGA	6 cut(s)	365, 836, 941, 1014, 1057, 1373
Hpy8I	GTNNAC	7 cut(s)	94, 444, 625, 644, 839, 865, 1228
Hpy99I	CGWCG	1 cut(s)	648
HpyAV	CCTTC	6 cut(s)	11, 62, 134, 244, 838, 1378
HpyCH4III	ACNGT	3 cut(s)	171, 378, 406
HpyCH4IV	ACGT	4 cut(s)	679, 695, 1107, 1215
HpyCH4V	TGCA	6 cut(s)	224, 482, 677, 737, 1199, 1343
HpyF10VI	GCNNNNNNNGC	2 cut(s)	323, 1218
HpyF3I	CTNAG	4 cut(s)	313, 429, 629, 719
HpySE526I	ACGT	4 cut(s)	679, 695, 1107, 1215
Hsp92I	GRCGYC	1 cut(s)	434
Hsp92II	CATG	6 cut(s)	450, 486, 747, 854, 1081, 1343
HspAI	GCGC	1 cut(s)	1316
KroI	GCCGGC	1 cut(s)	659
KroNI	GCCGGC	1 cut(s)	661
Ksp22I	TGATCA	1 cut(s)	913
KspAI	GTTAAC	1 cut(s)	865
Kzo9I	GATC	5 cut(s)	544, 913, 1016, 1233, 1327
LmnI	GCTCC	2 cut(s)	788, 905
Lsp1109I	GCAGC	4 cut(s)	528, 971, 1211, 1284
LweI	GCATC	1 cut(s)	664
MaeI	CTAG	2 cut(s)	1325, 1432
MaeII	ACGT	4 cut(s)	679, 695, 1107, 1215
MaeIII	GTNAC	6 cut(s)	207, 329, 486, 691, 739, 1085
MalI	GATC	5 cut(s)	546, 915, 1018, 1235, 1329
MboI	GATC	5 cut(s)	544, 913, 1016, 1233, 1327
MboII	GAAGA	4 cut(s)	49, 241, 887, 1322
MfeI	CAATTG	1 cut(s)	321
MflI	RGATCY	3 cut(s)	544, 1016, 1327
MhlI	GDGCHC	1 cut(s)	637
MlsI	TGGCCA	4 cut(s)	372, 585, 726, 1338
MluCI	AATT	6 cut(s)	237, 291, 321, 463, 831, 1190
MluNI	TGGCCA	4 cut(s)	372, 585, 726, 1338
MlyI	GAGTC	2 cut(s)	215, 1378
MmeI	TCCRAC	1 cut(s)	433
MnlI	CCTC	9 cut(s)	14, 19, 111, 151, 156, 693, 937, 1156, 1342
Mox20I	TGGCCA	4 cut(s)	372, 585, 726, 1338
MroNI	GCCGGC	1 cut(s)	659
MroXI	GAANNNNTTC	2 cut(s)	891, 1050
MscI	TGGCCA	4 cut(s)	372, 585, 726, 1338
MseI	TTAA	4 cut(s)	138, 423, 864, 1290
MslI	CAYNNNNRTG	3 cut(s)	630, 682, 1344
Msp20I	TGGCCA	4 cut(s)	372, 585, 726, 1338
MspA1I	CMGCKG	2 cut(s)	439, 1239
MspCI	CTTAAG	1 cut(s)	1289
MspI	CCGG	3 cut(s)	89, 660, 1207
MspR9I	CCNGG	3 cut(s)	788, 1208, 1335
MunI	CAATTG	1 cut(s)	321
MvaI	CCWGG	2 cut(s)	788, 1335
MwoI	GCNNNNNNNGC	2 cut(s)	323, 1218
NaeI	GCCGGC	1 cut(s)	661
NciI	CCSGG	1 cut(s)	1208
NdeII	GATC	5 cut(s)	544, 913, 1016, 1233, 1327
NgoMIV	GCCGGC	1 cut(s)	659
NlaIII	CATG	6 cut(s)	450, 486, 747, 854, 1081, 1343
NlaIV	GGNNCC	9 cut(s)	63, 419, 470, 546, 618, 784, 907, 1047, 1211
NmuCI	GTSAC	4 cut(s)	207, 329, 486, 1085
NspI	RCATGY	2 cut(s)	450, 747
OliI	CACNNNNGTG	1 cut(s)	630
PciI	ACATGT	2 cut(s)	446, 743
PdiI	GCCGGC	1 cut(s)	661
PdmI	GAANNNNTTC	2 cut(s)	891, 1050
PfeI	GAWTC	3 cut(s)	606, 992, 1280
Pfl23II	CGTACG	1 cut(s)	899
PflMI	CCANNNNNTGG	2 cut(s)	379, 581
PkrI	GCNGC	5 cut(s)	438, 518, 961, 1201, 1274
PleI	GAGTC	2 cut(s)	214, 1377
PpsI	GAGTC	2 cut(s)	214, 1377
Ppu21I	YACGTR	1 cut(s)	680
PpuMI	RGGWCCY	2 cut(s)	418, 469
PscI	ACATGT	2 cut(s)	446, 743
Psp5II	RGGWCCY	2 cut(s)	418, 469
Psp6I	CCWGG	2 cut(s)	786, 1333
PspEI	GGTNACC	2 cut(s)	486, 1085
PspGI	CCWGG	2 cut(s)	786, 1333
PspLI	CGTACG	1 cut(s)	899
PspN4I	GGNNCC	9 cut(s)	63, 419, 470, 546, 618, 784, 907, 1047, 1211
PspPI	GGNCC	5 cut(s)	61, 418, 469, 1210, 1220
PspPPI	RGGWCCY	2 cut(s)	418, 469
PstNI	CAGNNNCTG	1 cut(s)	341
PsuI	RGATCY	3 cut(s)	544, 1016, 1327
PvuII	CAGCTG	1 cut(s)	1239
RsaI	GTAC	6 cut(s)	345, 451, 796, 883, 901, 1180
RsaNI	GTAC	6 cut(s)	344, 450, 795, 882, 900, 1179
RseI	CAYNNNNRTG	3 cut(s)	630, 682, 1344
SalI	GTCGAC	1 cut(s)	642
SaqAI	TTAA	4 cut(s)	138, 423, 864, 1290
SatI	GCNGC	5 cut(s)	437, 517, 960, 1200, 1273
Sau3AI	GATC	5 cut(s)	544, 913, 1016, 1233, 1327
Sau96I	GGNCC	5 cut(s)	61, 418, 469, 1210, 1220
ScaI	AGTACT	1 cut(s)	883
SchI	GAGTC	2 cut(s)	215, 1378
ScrFI	CCNGG	3 cut(s)	788, 1208, 1335
SduI	GDGCHC	1 cut(s)	637
SfaNI	GCATC	1 cut(s)	664
SfiI	GGCCNNNNNGGCC	1 cut(s)	1218
SinI	GGWCC	3 cut(s)	61, 418, 469
SmiMI	CAYNNNNRTG	3 cut(s)	630, 682, 1344
SmlI	CTYRAG	2 cut(s)	1055, 1289
SmoI	CTYRAG	2 cut(s)	1055, 1289
Sse9I	AATT	6 cut(s)	237, 291, 321, 463, 831, 1190
SsiI	CCGC	2 cut(s)	116, 437
SspI	AATATT	1 cut(s)	932
SspMI	CTAG	2 cut(s)	1325, 1432
StyD4I	CCNGG	3 cut(s)	786, 1206, 1333
TaaI	ACNGT	3 cut(s)	171, 378, 406
TaiI	ACGT	4 cut(s)	682, 698, 1110, 1218
TaqI	TCGA	1 cut(s)	643
TasI	AATT	6 cut(s)	237, 291, 321, 463, 831, 1190
TatI	WGTACW	4 cut(s)	343, 449, 794, 881
TauI	GCSGC	1 cut(s)	439
TfiI	GAWTC	3 cut(s)	606, 992, 1280
Tru1I	TTAA	4 cut(s)	138, 423, 864, 1290
Tru9I	TTAA	4 cut(s)	138, 423, 864, 1290
TscAI	CASTG	3 cut(s)	115, 409, 925
TseFI	GTSAC	4 cut(s)	207, 329, 486, 1085
TseI	GCWGC	4 cut(s)	516, 959, 1199, 1272
Tsp45I	GTSAC	4 cut(s)	207, 329, 486, 1085
TspDTI	ATGAA	6 cut(s)	248, 293, 760, 924, 965, 998
TspGWI	ACGGA	1 cut(s)	74
TspRI	CASTG	3 cut(s)	115, 409, 925
Van91I	CCANNNNNTGG	2 cut(s)	379, 581
Vha464I	CTTAAG	1 cut(s)	1289
VpaK11BI	GGWCC	3 cut(s)	61, 418, 469
XapI	RAATTY	3 cut(s)	237, 291, 1190
XceI	RCATGY	2 cut(s)	450, 747
XmiI	GTMKAC	2 cut(s)	643, 1227
XmnI	GAANNNNTTC	2 cut(s)	891, 1050
XspI	CTAG	2 cut(s)	1325, 1432
ZrmI	AGTACT	1 cut(s)	883

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.