Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0011105

Physical Location & Seq

Genomic Coordinates Forward (+)

34725637 .. 34732781

Gene Structure 7145 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1287700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1428 bp

ATGGCTATGGAGGTGGAAGAAACAGAGGGGAAGACTGTGTTGTGCCTGCTGACGGACCCAGATGGGACTCCATTAGGAAGCTCCGTCTACCTTCCTCAGAACACTGAGCCGCAACACCTTCAGCAAATCGTCAATCAGCTTCTCAAAAATGAGGAGAGGCTACCTTATGCTTTCTATATACAAGATCAGGAACTTCTTGAGTCACTTGGAAAATATGCAGAGAAGAAGAAAATTTCCGTGGAGAAGGTACTCAACATAGTTTATCAACCACAAGCTGTTTTCCGAATTCGTCCTGTTCATCGTTGTTCAGCAACAATTGCTGGTCACACAGAAGCTGTACTTTCAGTTGCTTTTAGTCCTGATGGTCAACATTTGGCTAGTGGTTCTGGTGATACAACTGTCCGACTATGGGACCTTAATACTCAGACGCCGTTGCACACATGCACAGGACATAAGAATTGGGTCCTTTCTATTGCATGGTCACCTGATGGTAAGCATCTTGTTAGTGGCAGCAAGTCTGGAGAACTTCAATGTTGGGATCCACTGACAGGGAATCCATCAGGCAATCCACTTATCGGCCACAAGAAATGGATTACTGGAATCTCTTGGGAACCAGTCCACCTTAGCTCTCCGTGTCGACGCTTTGTAAGTGCTAGCAAAGATGGTGATGCACGCATATGGGATGTTACGTTGAGGAAATCTGTTATATGTCTTAGTGGCCACACACTTGCAGTAACTTGTGTAAAATGGGGTGGAGATGGTGTCATATATACAGGCTCCCAGGATTGTACCATCAAAGTATGGGAGACCACTCAAGGAAAACTAATTCGTGAACTGAAGGGTCATGGTCATTGGGTTAACTCTCTTACATTGAGTACTGAATATGTTCTTCGTACTGGAGCCTTTGATCACACTTGCAAGCAATATTCATCTCCAGAGGAAATGAAGAAGGCAGCTTTGGAAAGATATAACAAAATGAAAGGCAATGCCCCTGAAAGATTGGTTTCTGGATCCGATGATTTTACAATGTTTCTATGGGAACCTTTTGTCAGCAAACAACCTAAAACTCGCATGACAGGTCATCAACAGCTTGTAAACCACGTCTGCTTTTCACCTGATGGGCAATGGATAGCAAGTGCCTCATTTGATAGGTCTGTCAAGTTATGGAACTGTACTACTGGAAAATTTGTTGCAGCTTTCCGGGGCCATGTTGGCCCTGTCTACCAGATCAGCTGGTCGGCAGACAGTAGGCTGCTTGTTAGTGGCAGCAAAGATTCCACTCTCAAGGTTTGGGATATCAGGGCACGGGCGCTAAAACAAGACCTTCCAGGCCATGCTGATGAGGTTTTTGCTGTCGATTGGAGTCCAGATGGTGGGAAGGTTGCCTCGGGTGGCAAGGATAGAGTTCTGAAGCTATGGATGGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000027 GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005840 GO:0006996 GO:0007275 GO:0008150 GO:0009553 GO:0009987 GO:0016043 GO:0022607 GO:0022613 GO:0022618 GO:0031461 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0042254 GO:0042255 GO:0042273 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0065003 GO:0070013 GO:0070925 GO:0071826 GO:0071840 GO:0080008 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NLE WD40

Protein Analysis

476

Amino Acids

52.71

Weight (kDa)

8.38

Isoelectric Point (pI)

36.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NLE	PF08154	14 - 72	8.8e-13	NLE (NUC135) domain
Beta-prop_Aladin	PF25460	97 - 180	1.1e-07	Aladin seven-bladed propeller
WD40_CDC20-Fz	PF24807	100 - 166	2.6e-13	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	100 - 148	5.2e-09	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	101 - 184	2.8e-14	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	101 - 200	1.5e-20	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	101 - 203	9.4e-22	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	101 - 158	1.6e-13	THOC3 beta-propeller domain
WD40	PF00400	101 - 138	1.6e-12	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	102 - 180	3.1e-09	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	103 - 269	2.9e-18	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	103 - 150	2.6e-09	WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	104 - 171	1.6e-10	CAF1B/HIR1 beta-propeller domain
WDR55	PF24796	105 - 181	6.6e-08	WDR55
Beta-prop_EML_2	PF23414	105 - 186	7.1e-13	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_1st	PF24780	106 - 170	1.2e-06	MABP1/WDR62 first WD40 domain
Beta-prop_EML	PF23409	108 - 312	5.5e-09	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	110 - 180	6e-07	WDR90/POC16, second beta-propeller
WD40_Prp19	PF24814	141 - 309	1.2e-18	Prp19 WD40 domain
WD40	PF00400	143 - 180	4.2e-10	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	147 - 234	9.1e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	151 - 254	2.4e-15	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	152 - 211	7.5e-06	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	161 - 269	5e-11	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	191 - 309	1.7e-16	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	191 - 310	6.6e-18	WDR5 beta-propeller domain
WDR55	PF24796	207 - 295	4e-06	WDR55
Beta-prop_WDR3_2nd	PF25172	208 - 313	7.1e-07	WDR3 second beta-propeller domain
WD40_CDC20-Fz	PF24807	217 - 312	2e-08	CDC20/Fizzy WD40 domain
WD40	PF00400	233 - 268	8.6e-07	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	241 - 400	2.4e-13	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	241 - 310	2.3e-07	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	329 - 473	1.3e-15	TEP-1 second beta-propeller
WD40_Prp19	PF24814	329 - 473	6.4e-21	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	332 - 433	1e-22	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	332 - 398	3.4e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	332 - 438	2.2e-21	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	333 - 473	1.9e-34	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	345 - 473	4.3e-21	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	352 - 401	3.4e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	353 - 435	3e-11	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	354 - 390	2.4e-11	WD domain, G-beta repeat
WDR55	PF24796	356 - 452	1.8e-12	WDR55
Beta-prop_WDR36-Utp21_1st	PF25171	356 - 474	5.1e-07	WDR36/Utp21 first beta-propeller
EIF3I	PF24805	358 - 434	3.7e-12	EIF3I
Beta-prop_WDR3_2nd	PF25172	360 - 473	2e-17	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	361 - 473	4.3e-27	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	364 - 432	7.3e-08	MABP1/WDR62 second WD40 domain
WDR90_beta-prop_4th	PF23342	365 - 469	9.4e-10	WDR90, 4th beta-propeller
Beta-prop_WDR3_1st	PF25173	371 - 473	2.4e-23	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	379 - 472	2.3e-14	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	392 - 463	6.9e-08	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	394 - 432	3.9e-10	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	399 - 472	2.7e-11	CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin	PF25460	405 - 473	8.4e-08	Aladin seven-bladed propeller
WD40_WDHD1_1st	PF24817	406 - 473	7.4e-07	WDHD1 first WD40 domain
WD40	PF00400	439 - 473	1.1e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011105)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52820
fragaria_vesca	FvH4_7g01500
malus_domestica	MD02G1305600.v1.1 MD09G1280600.v1.1 MD17G1287700.v1.1
prunus_persica	Prupe.2G020500_v2.0.a1
pyrus_communis	pycom02g25610 pycom17g27070
rosa_chinensis	RchiOBHm_Chr1g0317321
rosa_laevigata	RLG00000029568 RLG00000030606
rosa_multiflora	Rmu_sc0005092.1_g000002 Rmu_sc0013869.1_g000006
rosa_roxburghii	Rroxscaffold_4G00330490
rosa_rugosa	Rorug01G0015900
rosa_samantha	Rh1AG027300 Rh1BG023200 Rh1CG025300 Rh1DG045900
rosa_wichuraiana	Rw1G001910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1373
AccI	GTMKAC	3 cut(s)	87, 637, 1221
AciI	CCGC	1 cut(s)	110
AclWI	GGATC	4 cut(s)	533, 546, 1005, 1018
AcoI	YGGCCR	2 cut(s)	577, 718
AcsI	RAATTY	3 cut(s)	231, 285, 1184
AcuI	CTGAAG	2 cut(s)	104, 857
AcyI	GRCGYC	1 cut(s)	428
AfaI	GTAC	6 cut(s)	249, 339, 790, 877, 895, 1174
AfiI	CCNNNNNNNGG	5 cut(s)	52, 409, 548, 575, 1373
AgsI	TTSAA	1 cut(s)	530
AjiI	CACGTC	1 cut(s)	1102
AjnI	CCWGG	2 cut(s)	780, 1327
AjuI	GAANNNNNNNTTGG	2 cut(s)	941, 973
Alw26I	GTCTC	1 cut(s)	800
AlwI	GGATC	4 cut(s)	533, 546, 1005, 1018
AlwNI	CAGNNNCTG	1 cut(s)	335
Ama87I	CYCGRG	1 cut(s)	1387
AoxI	GGCC	5 cut(s)	577, 718, 1204, 1213, 1330
ApeKI	GCWGC	5 cut(s)	510, 953, 1193, 1252, 1266
ApoI	RAATTY	3 cut(s)	231, 285, 1184
Asp700I	GAANNNNTTC	1 cut(s)	885
AspLEI	GCGC	1 cut(s)	1312
AspS9I	GGNCC	5 cut(s)	55, 412, 463, 1204, 1214
AsuC2I	CCSGG	1 cut(s)	1202
AsuHPI	GGTGA	4 cut(s)	401, 474, 677, 1104
AsuNHI	GCTAGC	1 cut(s)	653
AvaI	CYCGRG	1 cut(s)	1387
AvaII	GGWCC	3 cut(s)	55, 412, 463
BaeGI	GKGCMC	1 cut(s)	1306
BaeI	ACNNNNGTAYC	2 cut(s)	384, 417
BalI	TGGCCA	1 cut(s)	720
BamHI	GGATCC	2 cut(s)	538, 1010
BbsI	GAAGAC	1 cut(s)	38
BbvI	GCAGC	5 cut(s)	522, 965, 1205, 1239, 1278
BceAI	ACGGC	1 cut(s)	415
BciT130I	CCWGG	2 cut(s)	782, 1329
BclI	TGATCA	1 cut(s)	907
BcnI	CCSGG	1 cut(s)	1202
BcoDI	GTCTC	1 cut(s)	800
BfaI	CTAG	3 cut(s)	378, 654, 1426
BfoI	RGCGCY	1 cut(s)	1313
BglI	GCCNNNNNGGC	1 cut(s)	1212
BisI	GCNGC	6 cut(s)	110, 511, 954, 1194, 1253, 1267
BlsI	GCNGC	6 cut(s)	111, 512, 955, 1195, 1254, 1268
BmcAI	AGTACT	1 cut(s)	877
Bme1390I	CCNGG	3 cut(s)	782, 1202, 1329
Bme18I	GGWCC	3 cut(s)	55, 412, 463
BmeT110I	CYCGRG	1 cut(s)	1387
BmgBI	CACGTC	1 cut(s)	1102
BmgT120I	GGNCC	5 cut(s)	55, 412, 463, 1204, 1214
BmrFI	CCNGG	3 cut(s)	782, 1202, 1329
BmsI	GCATC	2 cut(s)	505, 658
BmtI	GCTAGC	1 cut(s)	657
BpiI	GAAGAC	1 cut(s)	38
BpmI	CTGGAG	3 cut(s)	540, 918, 918
Bpu10I	CCTNAGC	1 cut(s)	623
BpuEI	CTTGAG	3 cut(s)	218, 798, 1268
BpuMI	CCSGG	1 cut(s)	1202
BsaHI	GRCGYC	1 cut(s)	428
BsaI	GGTCTC	1 cut(s)	800
BsaJI	CCNNGG	4 cut(s)	237, 780, 1201, 1386
BsaXI	ACNNNNNCTCC	2 cut(s)	747, 777
Bsc4I	CCNNNNNNNGG	5 cut(s)	52, 409, 548, 575, 1373
Bse1I	ACTGG	4 cut(s)	601, 614, 901, 1183
Bse3DI	GCAATG	2 cut(s)	991, 1130
BseBI	CCWGG	2 cut(s)	782, 1329
BseDI	CCNNGG	4 cut(s)	237, 780, 1201, 1386
BseGI	GGATG	2 cut(s)	688, 1425
BseLI	CCNNNNNNNGG	5 cut(s)	52, 409, 548, 575, 1373
BseMI	GCAATG	2 cut(s)	991, 1130
BseMII	CTCAG	3 cut(s)	96, 110, 437
BseNI	ACTGG	4 cut(s)	601, 614, 901, 1183
BseRI	GAGGAG	1 cut(s)	167
BseSI	GKGCMC	1 cut(s)	1306
BseXI	GCAGC	5 cut(s)	522, 965, 1205, 1239, 1278
BshFI	GGCC	5 cut(s)	579, 720, 1206, 1215, 1332
BsiHKCI	CYCGRG	1 cut(s)	1387
BsiSI	CCGG	1 cut(s)	1201
BslFI	GGGAC	2 cut(s)	79, 425
BslI	CCNNNNNNNGG	5 cut(s)	52, 409, 548, 575, 1373
BsmAI	GTCTC	1 cut(s)	800
BsmFI	GGGAC	2 cut(s)	79, 425
BsnI	GGCC	5 cut(s)	579, 720, 1206, 1215, 1332
Bso31I	GGTCTC	1 cut(s)	800
BsoBI	CYCGRG	1 cut(s)	1387
Bsp1286I	GDGCHC	1 cut(s)	1306
Bsp143I	GATC	5 cut(s)	184, 538, 907, 1010, 1227
BspACI	CCGC	1 cut(s)	110
BspANI	GGCC	5 cut(s)	579, 720, 1206, 1215, 1332
BspCNI	CTCAG	3 cut(s)	97, 109, 436
BspOI	GCTAGC	1 cut(s)	657
BspPI	GGATC	4 cut(s)	533, 546, 1005, 1018
BspTNI	GGTCTC	1 cut(s)	800
BsrDI	GCAATG	2 cut(s)	991, 1130
BsrI	ACTGG	4 cut(s)	601, 614, 901, 1183
BssECI	CCNNGG	4 cut(s)	237, 780, 1201, 1386
BssMI	GATC	5 cut(s)	184, 538, 907, 1010, 1227
BssNI	GRCGYC	1 cut(s)	428
Bst2UI	CCWGG	2 cut(s)	782, 1329
Bst4CI	ACNGT	4 cut(s)	37, 400, 1172, 1247
BstACI	GRCGYC	1 cut(s)	428
BstAPI	GCANNNNNTGC	1 cut(s)	317
BstC8I	GCNNGC	4 cut(s)	47, 655, 673, 920
BstDEI	CTNAG	5 cut(s)	96, 105, 423, 623, 713
BstDSI	CCRYGG	1 cut(s)	237
BstEII	GGTNACC	1 cut(s)	480
BstF5I	GGATG	2 cut(s)	688, 1425
BstH2I	RGCGCY	1 cut(s)	1313
BstHHI	GCGC	1 cut(s)	1312
BstKTI	GATC	5 cut(s)	187, 541, 910, 1013, 1230
BstMAI	GTCTC	1 cut(s)	800
BstMBI	GATC	5 cut(s)	184, 538, 907, 1010, 1227
BstMWI	GCNNNNNNNGC	2 cut(s)	317, 1212
BstNI	CCWGG	2 cut(s)	782, 1329
BstNSI	RCATGY	1 cut(s)	444
BstPI	GGTNACC	1 cut(s)	480
BstSCI	CCNGG	3 cut(s)	780, 1200, 1327
BstSLI	GKGCMC	1 cut(s)	1306
BstV1I	GCAGC	5 cut(s)	522, 965, 1205, 1239, 1278
BstV2I	GAAGAC	1 cut(s)	38
BstX2I	RGATCY	2 cut(s)	538, 1010
BstYI	RGATCY	2 cut(s)	538, 1010
BsuRI	GGCC	5 cut(s)	579, 720, 1206, 1215, 1332
BtgI	CCRYGG	1 cut(s)	237
BtrI	CACGTC	1 cut(s)	1102
BtsCI	GGATG	2 cut(s)	688, 1425
BtsIMutI	CAGTG	2 cut(s)	102, 542
Cac8I	GCNNGC	4 cut(s)	47, 655, 673, 920
CaiI	CAGNNNCTG	1 cut(s)	335
CfoI	GCGC	1 cut(s)	1312
Cfr13I	GGNCC	5 cut(s)	55, 412, 463, 1204, 1214
CseI	GACGC	2 cut(s)	436, 648
Csp6I	GTAC	6 cut(s)	248, 338, 789, 876, 894, 1173
CviAII	CATG	6 cut(s)	441, 477, 845, 1072, 1208, 1334
CviQI	GTAC	6 cut(s)	248, 338, 789, 876, 894, 1173
DdeI	CTNAG	5 cut(s)	96, 105, 423, 623, 713
DpnI	GATC	5 cut(s)	186, 540, 909, 1012, 1229
DpnII	GATC	5 cut(s)	184, 538, 907, 1010, 1227
EaeI	YGGCCR	2 cut(s)	577, 718
Eco31I	GGTCTC	1 cut(s)	800
Eco32I	GATATC	1 cut(s)	1297
Eco47I	GGWCC	3 cut(s)	55, 412, 463
Eco57I	CTGAAG	2 cut(s)	104, 857
Eco88I	CYCGRG	1 cut(s)	1387
Eco91I	GGTNACC	1 cut(s)	480
EcoO109I	RGGNCCY	2 cut(s)	412, 463
EcoO65I	GGTNACC	1 cut(s)	480
EcoRI	GAATTC	1 cut(s)	285
EcoRII	CCWGG	2 cut(s)	780, 1327
EcoRV	GATATC	1 cut(s)	1297
FaeI	CATG	6 cut(s)	444, 480, 848, 1075, 1211, 1337
FalI	AAGNNNNNCTT	2 cut(s)	324, 356
FaqI	GGGAC	2 cut(s)	79, 425
FatI	CATG	6 cut(s)	440, 476, 844, 1071, 1207, 1333
FauNDI	CATATG	1 cut(s)	677
FbaI	TGATCA	1 cut(s)	907
FblI	GTMKAC	3 cut(s)	87, 637, 1221
Fnu4HI	GCNGC	6 cut(s)	110, 511, 954, 1194, 1253, 1267
FokI	GGATG	1 cut(s)	695
Fsp4HI	GCNGC	6 cut(s)	110, 511, 954, 1194, 1253, 1267
FspBI	CTAG	3 cut(s)	378, 654, 1426
GlaI	GCGC	1 cut(s)	1311
GluI	GCNGC	6 cut(s)	110, 511, 954, 1194, 1253, 1267
GsuI	CTGGAG	3 cut(s)	540, 918, 918
HaeII	RGCGCY	1 cut(s)	1313
HaeIII	GGCC	5 cut(s)	579, 720, 1206, 1215, 1332
HapII	CCGG	1 cut(s)	1201
HgaI	GACGC	2 cut(s)	436, 648
HhaI	GCGC	1 cut(s)	1312
Hin1I	GRCGYC	1 cut(s)	428
Hin1II	CATG	6 cut(s)	444, 480, 848, 1075, 1211, 1337
Hin6I	GCGC	1 cut(s)	1310
HinP1I	GCGC	1 cut(s)	1310
HincII	GTYRAC	3 cut(s)	368, 638, 859
HindII	GTYRAC	3 cut(s)	368, 638, 859
HinfI	GANTC	6 cut(s)	67, 200, 553, 600, 1274, 1363
HpaI	GTTAAC	1 cut(s)	859
HpaII	CCGG	1 cut(s)	1201
HphI	GGTGA	4 cut(s)	401, 474, 677, 1104
Hpy166II	GTNNAC	8 cut(s)	88, 368, 619, 638, 833, 859, 1096, 1222
Hpy188I	TCNGA	6 cut(s)	99, 284, 404, 426, 1015, 1410
Hpy188III	TCNNGA	8 cut(s)	188, 197, 359, 519, 830, 935, 1008, 1367
Hpy8I	GTNNAC	8 cut(s)	88, 368, 619, 638, 833, 859, 1096, 1222
Hpy99I	CGWCG	1 cut(s)	642
HpyAV	CCTTC	7 cut(s)	101, 128, 238, 832, 943, 1334, 1372
HpyCH4III	ACNGT	4 cut(s)	37, 400, 1172, 1247
HpyCH4IV	ACGT	2 cut(s)	689, 1101
HpyCH4V	TGCA	8 cut(s)	218, 436, 444, 476, 671, 731, 918, 1193
HpyF10VI	GCNNNNNNNGC	2 cut(s)	317, 1212
HpyF3I	CTNAG	5 cut(s)	96, 105, 423, 623, 713
HpySE526I	ACGT	2 cut(s)	689, 1101
Hsp92I	GRCGYC	1 cut(s)	428
Hsp92II	CATG	6 cut(s)	444, 480, 848, 1075, 1211, 1337
HspAI	GCGC	1 cut(s)	1310
Ksp22I	TGATCA	1 cut(s)	907
KspAI	GTTAAC	1 cut(s)	859
Kzo9I	GATC	5 cut(s)	184, 538, 907, 1010, 1227
LmnI	GCTCC	3 cut(s)	86, 782, 899
Lsp1109I	GCAGC	5 cut(s)	522, 965, 1205, 1239, 1278
LweI	GCATC	2 cut(s)	505, 658
MaeI	CTAG	3 cut(s)	378, 654, 1426
MaeII	ACGT	2 cut(s)	689, 1101
MaeIII	GTNAC	5 cut(s)	201, 323, 480, 685, 733
MalI	GATC	5 cut(s)	186, 540, 909, 1012, 1229
MboI	GATC	5 cut(s)	184, 538, 907, 1010, 1227
MboII	GAAGA	6 cut(s)	29, 43, 235, 238, 881, 958
MfeI	CAATTG	1 cut(s)	315
MflI	RGATCY	2 cut(s)	538, 1010
MhlI	GDGCHC	1 cut(s)	1306
MlsI	TGGCCA	1 cut(s)	720
MluCI	AATT	6 cut(s)	231, 285, 315, 457, 825, 1184
MluNI	TGGCCA	1 cut(s)	720
MlyI	GAGTC	3 cut(s)	61, 209, 1372
MmeI	TCCRAC	1 cut(s)	427
Mox20I	TGGCCA	1 cut(s)	720
MroXI	GAANNNNTTC	1 cut(s)	885
MscI	TGGCCA	1 cut(s)	720
MseI	TTAA	2 cut(s)	417, 858
MslI	CAYNNNNRTG	2 cut(s)	676, 1338
Msp20I	TGGCCA	1 cut(s)	720
MspA1I	CMGCKG	1 cut(s)	1233
MspI	CCGG	1 cut(s)	1201
MspR9I	CCNGG	3 cut(s)	782, 1202, 1329
MunI	CAATTG	1 cut(s)	315
MvaI	CCWGG	2 cut(s)	782, 1329
MwoI	GCNNNNNNNGC	2 cut(s)	317, 1212
NciI	CCSGG	1 cut(s)	1202
NdeI	CATATG	1 cut(s)	677
NdeII	GATC	5 cut(s)	184, 538, 907, 1010, 1227
NheI	GCTAGC	1 cut(s)	653
NlaIII	CATG	6 cut(s)	444, 480, 848, 1075, 1211, 1337
NmuCI	GTSAC	3 cut(s)	201, 323, 480
NspI	RCATGY	1 cut(s)	444
PdmI	GAANNNNTTC	1 cut(s)	885
PfeI	GAWTC	3 cut(s)	553, 600, 1274
PflMI	CCANNNNNTGG	1 cut(s)	1373
PkrI	GCNGC	6 cut(s)	111, 512, 955, 1195, 1254, 1268
PleI	GAGTC	3 cut(s)	61, 208, 1371
PpsI	GAGTC	3 cut(s)	61, 208, 1371
PpuMI	RGGWCCY	2 cut(s)	412, 463
Psp5II	RGGWCCY	2 cut(s)	412, 463
Psp6I	CCWGG	2 cut(s)	780, 1327
PspEI	GGTNACC	1 cut(s)	480
PspGI	CCWGG	2 cut(s)	780, 1327
PspPI	GGNCC	5 cut(s)	55, 412, 463, 1204, 1214
PspPPI	RGGWCCY	2 cut(s)	412, 463
PstNI	CAGNNNCTG	1 cut(s)	335
PsuI	RGATCY	2 cut(s)	538, 1010
PvuII	CAGCTG	1 cut(s)	1233
RsaI	GTAC	6 cut(s)	249, 339, 790, 877, 895, 1174
RsaNI	GTAC	6 cut(s)	248, 338, 789, 876, 894, 1173
RseI	CAYNNNNRTG	2 cut(s)	676, 1338
SalI	GTCGAC	1 cut(s)	636
SaqAI	TTAA	2 cut(s)	417, 858
SatI	GCNGC	6 cut(s)	110, 511, 954, 1194, 1253, 1267
Sau3AI	GATC	5 cut(s)	184, 538, 907, 1010, 1227
Sau96I	GGNCC	5 cut(s)	55, 412, 463, 1204, 1214
ScaI	AGTACT	1 cut(s)	877
SchI	GAGTC	3 cut(s)	61, 209, 1372
ScrFI	CCNGG	3 cut(s)	782, 1202, 1329
SduI	GDGCHC	1 cut(s)	1306
SfaNI	GCATC	2 cut(s)	505, 658
SfiI	GGCCNNNNNGGCC	1 cut(s)	1212
SinI	GGWCC	3 cut(s)	55, 412, 463
SmiMI	CAYNNNNRTG	2 cut(s)	676, 1338
SmlI	CTYRAG	3 cut(s)	197, 813, 1283
SmoI	CTYRAG	3 cut(s)	197, 813, 1283
Sse9I	AATT	6 cut(s)	231, 285, 315, 457, 825, 1184
SsiI	CCGC	1 cut(s)	110
SspI	AATATT	1 cut(s)	926
SspMI	CTAG	3 cut(s)	378, 654, 1426
StyD4I	CCNGG	3 cut(s)	780, 1200, 1327
TaaI	ACNGT	4 cut(s)	37, 400, 1172, 1247
TaiI	ACGT	2 cut(s)	692, 1104
TaqI	TCGA	2 cut(s)	637, 1356
TasI	AATT	6 cut(s)	231, 285, 315, 457, 825, 1184
TatI	WGTACW	3 cut(s)	337, 875, 1172
TauI	GCSGC	1 cut(s)	112
TfiI	GAWTC	3 cut(s)	553, 600, 1274
Tru1I	TTAA	2 cut(s)	417, 858
Tru9I	TTAA	2 cut(s)	417, 858
TscAI	CASTG	2 cut(s)	109, 549
TseFI	GTSAC	3 cut(s)	201, 323, 480
TseI	GCWGC	5 cut(s)	510, 953, 1193, 1252, 1266
Tsp45I	GTSAC	3 cut(s)	201, 323, 480
TspDTI	ATGAA	4 cut(s)	287, 918, 959, 992
TspGWI	ACGGA	4 cut(s)	68, 73, 226, 621
TspRI	CASTG	2 cut(s)	109, 549
Van91I	CCANNNNNTGG	1 cut(s)	1373
VpaK11BI	GGWCC	3 cut(s)	55, 412, 463
XapI	RAATTY	3 cut(s)	231, 285, 1184
XceI	RCATGY	1 cut(s)	444
XmiI	GTMKAC	3 cut(s)	87, 637, 1221
XmnI	GAANNNNTTC	1 cut(s)	885
XspI	CTAG	3 cut(s)	378, 654, 1426
ZrmI	AGTACT	1 cut(s)	877

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.