Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0011105

Physical Location & Seq

Genomic Coordinates Reverse (-)

7483789 .. 7490364

Gene Structure 6576 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG045900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGCCATGGAAGACGAAGCAGCGGAGAAGAACGTGATGTGTCTTCTGACGGACCCAGAAGATGCCCCATTAGGAAAGTCTTTGTATCTTCCTCAACACACTGGCACCCAGCAACTTCAACAAATCGTCAATCAGCTTCTCAACAATGAGGATAAGGTGCCTTATACTTTCTATATAGCAGATAAGGAACTTAATCAGTCGCTTGGCGAATATGCGGAGAAGAATAAAATTTCTGTGGAGAAGGTACTCAAAATCGTTTATCAACCACAAGCTATTTTCCGAATTCGTCCCGTCAATCGTTGCTCGGCCACAATTGCTGGTCACTCAGAAGCTGTACTTTCAGTTGCTTTTAGTCCTGATGGTCGACAGTTGGCGAGTGGTTCTGGTGATACCACTGTCCGACTATGGGACCTTAATACTCAGACACCTTTGCACACGTGTTCAGGACATAAGAATTGGGTTCTTTGTATTGCATGGTCTGCTGATGGCAAGTATCTTGTTAGTGGGAGCAAGTCTGGAGAACTTCAATGTTGGGATCCACAGACAGGAAAACCAGCAGGCAATCCACTTACTGGCCACAAGAAATGGATTACTGGAATCTCTTGGGAACCAGTCCACCTTAGTGCTCCATGTAGACGCTTTGTAAGTGCTGGGAAAGATGGTGATGCACGCATATGGGACATCACGTTGAAGAAATGTGTTATATGTCTTAGTGGCCACACACTTGCAATAACTTGTGTAAAATGGGGTGGAGATGGTTTTATATATACAGGTTCCCAAGATTGTACAATTAAGGTCTGGGAGACAACACAGGGGAAGCTGATTCGTGAGCTAAAGGGTCATGGGCACTGGGTTAACTCTCTTGCATTGAGTACTGAATATGTTCTTCGCACTGGAGCTTATGATCACACTGGCAAGCAATATTCATCTCCTGAGGAAATGAAAAAGGTAGCTTTGGAAAGGTATAACAAAATGAAAGGTAAAGCTCCTGAACGATTGGTTTCTGGATCTGATGACTTTACTATGTTTCTATGGGAGCCTTTTGTCAGCAAACAACCCAAAGCTCGCATGACAGGTCATCAACAGCTTGTAAACCATGTCTGCTTTTCACCTGATGGGCAATGGATAGCAAGTGCCTCATTTGATAGGTCTGTCAGGATATGGAATGGTACTACTGGGAAGTTTTTTGGAGTTCTCCGGGGCCATGTTGGTGCTGTTTACCAGATCAGCTGGGCCGCAGACAGCAGGCTACTTTTAAGTGCTAGCAAAGATTCAACTCTGAAGGTTTGGGATATCAAGACTATGAAATTAAAACAAGACCTTCCAGGTCATGCAGATGAGGTTTATGCTGTTGATTGGAGTCCAGTCGGCGAAAAGGTTGCTTCGGGTGGTAAGGATAGAGTTTTGAAGTTATGGATGGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000027 GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005840 GO:0006996 GO:0007275 GO:0008150 GO:0009553 GO:0009987 GO:0016043 GO:0022607 GO:0022613 GO:0022618 GO:0031461 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0042254 GO:0042255 GO:0042273 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0065003 GO:0070013 GO:0070925 GO:0071826 GO:0071840 GO:0080008 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NLE WD40

Protein Analysis

474

Amino Acids

52.63

Weight (kDa)

8.69

Isoelectric Point (pI)

29.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NLE	PF08154	12 - 71	1.5e-13	NLE (NUC135) domain
Beta-prop_Aladin	PF25460	96 - 182	3e-07	Aladin seven-bladed propeller
WD40_Prp19	PF24814	99 - 146	2e-08	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	99 - 200	6.5e-21	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	100 - 234	8.7e-28	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	100 - 227	9.3e-18	TEP-1 second beta-propeller
WD40_CDC20-Fz	PF24807	100 - 160	6.2e-12	CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	100 - 138	8.8e-06	MABP1/WDR62 second WD40 domain
WD40	PF00400	100 - 137	4.8e-12	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	101 - 149	8.2e-09	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	101 - 179	3.7e-10	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	101 - 184	1.7e-20	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	102 - 240	3.2e-11	G protein beta WD-40 repeat protein
WDR55	PF24796	103 - 186	1.1e-08	WDR55
WD40_MABP1-WDR62_1st	PF24780	104 - 169	2.9e-08	MABP1/WDR62 first WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	104 - 154	6.3e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2	PF23414	104 - 189	1e-14	Echinoderm microtubule-associated protein second beta-propeller
ANAPC4_WD40	PF12894	105 - 160	1.4e-06	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_EML	PF23409	107 - 316	1.5e-12	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_IFT140_1st	PF23383	108 - 193	1.6e-06	IFT140 first beta-propeller
Beta-prop_WDR19_1st	PF23389	110 - 203	1.8e-07	WDR19 first beta-propeller
Beta-prop_IFT122_1st	PF23381	112 - 253	1.4e-07	IFT122 first beta-propeller
WD40_Prp19	PF24814	141 - 304	1.5e-19	Prp19 WD40 domain
WD40	PF00400	142 - 179	7.4e-10	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	146 - 234	1.6e-07	CAF1B/HIR1 beta-propeller domain
WD40_Gbeta	PF25391	146 - 315	1.4e-10	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	149 - 247	6.8e-11	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	149 - 204	3.6e-07	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	150 - 253	6.2e-17	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	152 - 212	1.4e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	190 - 303	4.1e-17	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	190 - 308	1.9e-16	WDR3 first beta-propeller domain
WDR55	PF24796	208 - 434	5.6e-16	WDR55
Beta-prop_WDR3_2nd	PF25172	211 - 310	1.3e-06	WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	211 - 299	1.3e-06	TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	211 - 394	1.7e-09	CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz	PF24807	218 - 306	4.4e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	223 - 292	3.6e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	232 - 267	3.2e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	240 - 306	4.1e-07	THOC3 beta-propeller domain
WD40_Prp19	PF24814	326 - 472	9.1e-20	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	331 - 472	2.5e-16	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	331 - 439	3.9e-20	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	331 - 394	3.4e-07	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	331 - 472	8.7e-29	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	332 - 406	1.8e-14	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	352 - 437	2e-11	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	352 - 389	1.6e-11	WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd	PF25168	354 - 395	5.6e-07	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	356 - 472	1.1e-17	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	357 - 472	8.6e-32	WDR5 beta-propeller domain
EIF3I	PF24805	357 - 436	3.7e-13	EIF3I
Beta-prop_WDR3_2nd	PF25172	357 - 472	7e-19	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	358 - 468	3.1e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3	PF25174	360 - 472	4.4e-26	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	364 - 431	5.1e-07	MABP1/WDR62 second WD40 domain
WDR90_beta-prop_4th	PF23342	364 - 468	2.4e-07	WDR90, 4th beta-propeller
WD40	PF00400	394 - 431	3.3e-08	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	397 - 471	2.9e-12	CAF1B/HIR1 beta-propeller domain
WD40_Gbeta	PF25391	398 - 470	1.8e-11	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	398 - 466	2.2e-07	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	403 - 472	2e-06	WDHD1 first WD40 domain
Beta-prop_Aladin	PF25460	404 - 472	8.6e-07	Aladin seven-bladed propeller
WD40	PF00400	436 - 472	2.5e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

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Orthologous Genes (Group: OG0011105)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52820
fragaria_vesca	FvH4_7g01500
malus_domestica	MD02G1305600.v1.1 MD09G1280600.v1.1 MD17G1287700.v1.1
prunus_persica	Prupe.2G020500_v2.0.a1
pyrus_communis	pycom02g25610 pycom17g27070
rosa_chinensis	RchiOBHm_Chr1g0317321
rosa_laevigata	RLG00000029568 RLG00000030606
rosa_multiflora	Rmu_sc0005092.1_g000002 Rmu_sc0013869.1_g000006
rosa_roxburghii	Rroxscaffold_4G00330490
rosa_rugosa	Rorug01G0015900
rosa_samantha	Rh1AG027300 Rh1BG023200 Rh1CG025300 Rh1DG045900
rosa_wichuraiana	Rw1G001910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	104, 157
AccB7I	CCANNNNNTGG	1 cut(s)	572
AccI	GTMKAC	2 cut(s)	364, 634
AciI	CCGC	3 cut(s)	23, 215, 1236
AclWI	GGATC	3 cut(s)	530, 543, 1015
AcoI	YGGCCR	4 cut(s)	3, 306, 574, 715
AcsI	RAATTY	2 cut(s)	228, 282
AcuI	CTGAAG	1 cut(s)	1301
AcvI	CACGTG	1 cut(s)	438
AfaI	GTAC	5 cut(s)	246, 336, 787, 874, 1171
AfiI	CCNNNNNNNGG	3 cut(s)	406, 545, 572
AflIII	ACRYGT	2 cut(s)	435, 437
AgsI	TTSAA	5 cut(s)	119, 527, 691, 1275, 1408
AjnI	CCWGG	1 cut(s)	1324
AleI	CACNNNNGTG	1 cut(s)	621
Alw21I	GWGCWC	1 cut(s)	628
Alw26I	GTCTC	1 cut(s)	797
AlwI	GGATC	3 cut(s)	530, 543, 1015
AlwNI	CAGNNNCTG	1 cut(s)	332
AoxI	GGCC	6 cut(s)	3, 306, 574, 715, 1201, 1233
ApeKI	GCWGC	1 cut(s)	20
ApoI	RAATTY	2 cut(s)	228, 282
Asp700I	GAANNNNTTC	1 cut(s)	882
AspS9I	GGNCC	4 cut(s)	52, 409, 1201, 1233
AsuC2I	CCSGG	1 cut(s)	1199
AsuHPI	GGTGA	3 cut(s)	398, 674, 1101
AsuNHI	GCTAGC	1 cut(s)	1262
AvaII	GGWCC	2 cut(s)	52, 409
AxyI	CCTNAGG	1 cut(s)	933
BaeGI	GKGCMC	1 cut(s)	849
BaeI	ACNNNNGTAYC	2 cut(s)	381, 414
BalI	TGGCCA	3 cut(s)	5, 576, 717
BamHI	GGATCC	1 cut(s)	535
BanI	GGYRCC	2 cut(s)	104, 157
BbrPI	CACGTG	1 cut(s)	438
BbsI	GAAGAC	2 cut(s)	18, 35
Bbv12I	GWGCWC	1 cut(s)	628
BbvI	GCAGC	1 cut(s)	32
BccI	CCATC	6 cut(s)	353, 479, 653, 749, 1109, 1411
BcgI	CGANNNNNNTGC	2 cut(s)	1361, 1395
BciT130I	CCWGG	1 cut(s)	1326
BclI	TGATCA	1 cut(s)	904
BcnI	CCSGG	1 cut(s)	1199
BcoDI	GTCTC	1 cut(s)	797
BfaI	CTAG	1 cut(s)	1263
BisI	GCNGC	2 cut(s)	21, 1236
BlsI	GCNGC	2 cut(s)	22, 1237
BmcAI	AGTACT	1 cut(s)	874
Bme1390I	CCNGG	2 cut(s)	1199, 1326
Bme18I	GGWCC	2 cut(s)	52, 409
BmgT120I	GGNCC	4 cut(s)	52, 409, 1201, 1233
BmiI	GGNNCC	9 cut(s)	54, 106, 159, 410, 537, 609, 775, 1038, 1202
BmrFI	CCNGG	2 cut(s)	1199, 1326
BmrI	ACTGGG	2 cut(s)	859, 1185
BmsI	GCATC	2 cut(s)	52, 655
BmtI	GCTAGC	1 cut(s)	1266
BmuI	ACTGGG	2 cut(s)	859, 1185
BpiI	GAAGAC	2 cut(s)	18, 35
BpmI	CTGGAG	2 cut(s)	537, 915
BpuMI	CCSGG	1 cut(s)	1199
BsaAI	YACGTR	1 cut(s)	438
BsaJI	CCNNGG	2 cut(s)	6, 1198
Bsc4I	CCNNNNNNNGG	3 cut(s)	406, 545, 572
Bse1I	ACTGG	9 cut(s)	106, 577, 598, 611, 854, 898, 916, 1180, 1364
Bse21I	CCTNAGG	1 cut(s)	933
Bse3DI	GCAATG	1 cut(s)	1127
BseBI	CCWGG	1 cut(s)	1326
BseDI	CCNNGG	2 cut(s)	6, 1198
BseGI	GGATG	1 cut(s)	1422
BseLI	CCNNNNNNNGG	3 cut(s)	406, 545, 572
BseMI	GCAATG	1 cut(s)	1127
BseMII	CTCAG	3 cut(s)	339, 434, 924
BseNI	ACTGG	9 cut(s)	106, 577, 598, 611, 854, 898, 916, 1180, 1364
BseSI	GKGCMC	1 cut(s)	849
BseXI	GCAGC	1 cut(s)	32
BseYI	CCCAGC	3 cut(s)	108, 650, 1230
BshFI	GGCC	6 cut(s)	5, 308, 576, 717, 1203, 1235
BshNI	GGYRCC	2 cut(s)	104, 157
BsiHKAI	GWGCWC	1 cut(s)	628
BsiSI	CCGG	1 cut(s)	1198
BslFI	GGGAC	3 cut(s)	273, 422, 692
BslI	CCNNNNNNNGG	3 cut(s)	406, 545, 572
BsmAI	GTCTC	1 cut(s)	797
BsmFI	GGGAC	3 cut(s)	273, 422, 692
BsnI	GGCC	6 cut(s)	5, 308, 576, 717, 1203, 1235
Bsp1286I	GDGCHC	2 cut(s)	628, 849
Bsp1407I	TGTACA	1 cut(s)	785
Bsp143I	GATC	4 cut(s)	535, 904, 1007, 1224
Bsp19I	CCATGG	1 cut(s)	6
BspACI	CCGC	3 cut(s)	23, 215, 1236
BspANI	GGCC	6 cut(s)	5, 308, 576, 717, 1203, 1235
BspCNI	CTCAG	3 cut(s)	338, 433, 925
BspLI	GGNNCC	9 cut(s)	54, 106, 159, 410, 537, 609, 775, 1038, 1202
BspOI	GCTAGC	1 cut(s)	1266
BspPI	GGATC	3 cut(s)	530, 543, 1015
BspT107I	GGYRCC	2 cut(s)	104, 157
BsrDI	GCAATG	1 cut(s)	1127
BsrGI	TGTACA	1 cut(s)	785
BsrI	ACTGG	9 cut(s)	106, 577, 598, 611, 854, 898, 916, 1180, 1364
BssECI	CCNNGG	2 cut(s)	6, 1198
BssMI	GATC	4 cut(s)	535, 904, 1007, 1224
BssT1I	CCWWGG	1 cut(s)	6
Bst2UI	CCWGG	1 cut(s)	1326
Bst4CI	ACNGT	2 cut(s)	369, 397
BstAPI	GCANNNNNTGC	1 cut(s)	479
BstAUI	TGTACA	1 cut(s)	785
BstBAI	YACGTR	1 cut(s)	438
BstC8I	GCNNGC	6 cut(s)	559, 670, 917, 1066, 1247, 1264
BstDEI	CTNAG	5 cut(s)	325, 420, 620, 710, 933
BstDSI	CCRYGG	1 cut(s)	6
BstF5I	GGATG	1 cut(s)	1422
BstKTI	GATC	4 cut(s)	538, 907, 1010, 1227
BstMAI	GTCTC	1 cut(s)	797
BstMBI	GATC	4 cut(s)	535, 904, 1007, 1224
BstMWI	GCNNNNNNNGC	2 cut(s)	314, 479
BstNI	CCWGG	1 cut(s)	1326
BstSCI	CCNGG	2 cut(s)	1197, 1324
BstSLI	GKGCMC	1 cut(s)	849
BstV1I	GCAGC	1 cut(s)	32
BstV2I	GAAGAC	2 cut(s)	18, 35
BstX2I	RGATCY	2 cut(s)	535, 1007
BstYI	RGATCY	2 cut(s)	535, 1007
Bsu36I	CCTNAGG	1 cut(s)	933
BsuRI	GGCC	6 cut(s)	5, 308, 576, 717, 1203, 1235
BtgI	CCRYGG	1 cut(s)	6
BtsCI	GGATG	1 cut(s)	1422
BtsIMutI	CAGTG	5 cut(s)	99, 393, 847, 891, 909
Cac8I	GCNNGC	6 cut(s)	559, 670, 917, 1066, 1247, 1264
CaiI	CAGNNNCTG	1 cut(s)	332
Cfr13I	GGNCC	4 cut(s)	52, 409, 1201, 1233
CseI	GACGC	1 cut(s)	645
Csp6I	GTAC	5 cut(s)	245, 335, 786, 873, 1170
CviAII	CATG	8 cut(s)	7, 474, 630, 842, 1069, 1097, 1205, 1331
CviQI	GTAC	5 cut(s)	245, 335, 786, 873, 1170
DdeI	CTNAG	5 cut(s)	325, 420, 620, 710, 933
DpnI	GATC	4 cut(s)	537, 906, 1009, 1226
DpnII	GATC	4 cut(s)	535, 904, 1007, 1224
EaeI	YGGCCR	4 cut(s)	3, 306, 574, 715
Eco130I	CCWWGG	1 cut(s)	6
Eco32I	GATATC	1 cut(s)	1294
Eco47I	GGWCC	2 cut(s)	52, 409
Eco57I	CTGAAG	1 cut(s)	1301
Eco72I	CACGTG	1 cut(s)	438
Eco81I	CCTNAGG	1 cut(s)	933
EcoO109I	RGGNCCY	1 cut(s)	409
EcoRI	GAATTC	1 cut(s)	282
EcoRII	CCWGG	1 cut(s)	1324
EcoRV	GATATC	1 cut(s)	1294
EcoT14I	CCWWGG	1 cut(s)	6
ErhI	CCWWGG	1 cut(s)	6
FaeI	CATG	8 cut(s)	10, 477, 633, 845, 1072, 1100, 1208, 1334
FalI	AAGNNNNNCTT	2 cut(s)	321, 353
FaqI	GGGAC	3 cut(s)	273, 422, 692
FatI	CATG	8 cut(s)	6, 473, 629, 841, 1068, 1096, 1204, 1330
FauNDI	CATATG	1 cut(s)	674
FbaI	TGATCA	1 cut(s)	904
FblI	GTMKAC	2 cut(s)	364, 634
Fnu4HI	GCNGC	2 cut(s)	21, 1236
Fsp4HI	GCNGC	2 cut(s)	21, 1236
FspBI	CTAG	1 cut(s)	1263
GluI	GCNGC	2 cut(s)	21, 1236
GsaI	CCCAGC	3 cut(s)	112, 654, 1234
GsuI	CTGGAG	2 cut(s)	537, 915
HaeIII	GGCC	6 cut(s)	5, 308, 576, 717, 1203, 1235
HapII	CCGG	1 cut(s)	1198
HgaI	GACGC	1 cut(s)	645
Hin1II	CATG	8 cut(s)	10, 477, 633, 845, 1072, 1100, 1208, 1334
HincII	GTYRAC	2 cut(s)	365, 856
HindII	GTYRAC	2 cut(s)	365, 856
HinfI	GANTC	4 cut(s)	597, 823, 1271, 1360
HpaI	GTTAAC	1 cut(s)	856
HpaII	CCGG	1 cut(s)	1198
HphI	GGTGA	3 cut(s)	398, 674, 1101
Hpy166II	GTNNAC	6 cut(s)	365, 616, 635, 856, 1093, 1219
Hpy188I	TCNGA	7 cut(s)	48, 281, 328, 401, 423, 1012, 1281
Hpy188III	TCNNGA	9 cut(s)	356, 444, 516, 827, 932, 989, 1005, 1156, 1297
Hpy8I	GTNNAC	6 cut(s)	365, 616, 635, 856, 1093, 1219
HpyAV	CCTTC	3 cut(s)	235, 1276, 1331
HpyCH4III	ACNGT	2 cut(s)	369, 397
HpyCH4IV	ACGT	3 cut(s)	33, 437, 686
HpyCH4V	TGCA	6 cut(s)	433, 473, 668, 728, 866, 1334
HpyF10VI	GCNNNNNNNGC	2 cut(s)	314, 479
HpyF3I	CTNAG	5 cut(s)	325, 420, 620, 710, 933
HpySE526I	ACGT	3 cut(s)	33, 437, 686
Hsp92II	CATG	8 cut(s)	10, 477, 633, 845, 1072, 1100, 1208, 1334
Ksp22I	TGATCA	1 cut(s)	904
KspAI	GTTAAC	1 cut(s)	856
Kzo9I	GATC	4 cut(s)	535, 904, 1007, 1224
LmnI	GCTCC	5 cut(s)	507, 631, 896, 991, 1036
Lsp1109I	GCAGC	1 cut(s)	32
LweI	GCATC	2 cut(s)	52, 655
MaeI	CTAG	1 cut(s)	1263
MaeII	ACGT	3 cut(s)	33, 437, 686
MaeIII	GTNAC	1 cut(s)	320
MalI	GATC	4 cut(s)	537, 906, 1009, 1226
MboI	GATC	4 cut(s)	535, 904, 1007, 1224
MboII	GAAGA	8 cut(s)	23, 35, 40, 71, 80, 232, 703, 878
MfeI	CAATTG	1 cut(s)	312
MflI	RGATCY	2 cut(s)	535, 1007
MhlI	GDGCHC	2 cut(s)	628, 849
MlsI	TGGCCA	3 cut(s)	5, 576, 717
MluCI	AATT	6 cut(s)	228, 282, 312, 454, 789, 1307
MluNI	TGGCCA	3 cut(s)	5, 576, 717
MlyI	GAGTC	1 cut(s)	1369
MmeI	TCCRAC	1 cut(s)	424
MnlI	CCTC	5 cut(s)	102, 142, 928, 1147, 1333
Mox20I	TGGCCA	3 cut(s)	5, 576, 717
MroXI	GAANNNNTTC	1 cut(s)	882
MscI	TGGCCA	3 cut(s)	5, 576, 717
MseI	TTAA	6 cut(s)	192, 414, 792, 855, 1256, 1310
MslI	CAYNNNNRTG	4 cut(s)	621, 673, 1209, 1335
Msp20I	TGGCCA	3 cut(s)	5, 576, 717
MspA1I	CMGCKG	2 cut(s)	23, 1230
MspI	CCGG	1 cut(s)	1198
MspR9I	CCNGG	2 cut(s)	1199, 1326
MunI	CAATTG	1 cut(s)	312
MvaI	CCWGG	1 cut(s)	1326
MwoI	GCNNNNNNNGC	2 cut(s)	314, 479
NciI	CCSGG	1 cut(s)	1199
NcoI	CCATGG	1 cut(s)	6
NdeI	CATATG	1 cut(s)	674
NdeII	GATC	4 cut(s)	535, 904, 1007, 1224
NheI	GCTAGC	1 cut(s)	1262
NlaIII	CATG	8 cut(s)	10, 477, 633, 845, 1072, 1100, 1208, 1334
NlaIV	GGNNCC	9 cut(s)	54, 106, 159, 410, 537, 609, 775, 1038, 1202
NmeAIII	GCCGAG	1 cut(s)	284
NmuCI	GTSAC	1 cut(s)	320
OliI	CACNNNNGTG	1 cut(s)	621
PdmI	GAANNNNTTC	1 cut(s)	882
PfeI	GAWTC	3 cut(s)	597, 823, 1271
PflMI	CCANNNNNTGG	1 cut(s)	572
PkrI	GCNGC	2 cut(s)	22, 1237
PleI	GAGTC	1 cut(s)	1368
PmaCI	CACGTG	1 cut(s)	438
PmlI	CACGTG	1 cut(s)	438
PpsI	GAGTC	1 cut(s)	1368
Ppu21I	YACGTR	1 cut(s)	438
PpuMI	RGGWCCY	1 cut(s)	409
Psp5II	RGGWCCY	1 cut(s)	409
Psp6I	CCWGG	1 cut(s)	1324
PspCI	CACGTG	1 cut(s)	438
PspFI	CCCAGC	3 cut(s)	108, 650, 1230
PspGI	CCWGG	1 cut(s)	1324
PspN4I	GGNNCC	9 cut(s)	54, 106, 159, 410, 537, 609, 775, 1038, 1202
PspPI	GGNCC	4 cut(s)	52, 409, 1201, 1233
PspPPI	RGGWCCY	1 cut(s)	409
PstNI	CAGNNNCTG	1 cut(s)	332
PsuI	RGATCY	2 cut(s)	535, 1007
PvuII	CAGCTG	1 cut(s)	1230
RsaI	GTAC	5 cut(s)	246, 336, 787, 874, 1171
RsaNI	GTAC	5 cut(s)	245, 335, 786, 873, 1170
RseI	CAYNNNNRTG	4 cut(s)	621, 673, 1209, 1335
SalI	GTCGAC	1 cut(s)	363
SaqAI	TTAA	6 cut(s)	192, 414, 792, 855, 1256, 1310
SatI	GCNGC	2 cut(s)	21, 1236
Sau3AI	GATC	4 cut(s)	535, 904, 1007, 1224
Sau96I	GGNCC	4 cut(s)	52, 409, 1201, 1233
ScaI	AGTACT	1 cut(s)	874
SchI	GAGTC	1 cut(s)	1369
ScrFI	CCNGG	2 cut(s)	1199, 1326
SduI	GDGCHC	2 cut(s)	628, 849
SfaNI	GCATC	2 cut(s)	52, 655
SinI	GGWCC	2 cut(s)	52, 409
SmiMI	CAYNNNNRTG	4 cut(s)	621, 673, 1209, 1335
Sse9I	AATT	6 cut(s)	228, 282, 312, 454, 789, 1307
SsiI	CCGC	3 cut(s)	23, 215, 1236
SspI	AATATT	1 cut(s)	923
SspMI	CTAG	1 cut(s)	1263
StyD4I	CCNGG	2 cut(s)	1197, 1324
StyI	CCWWGG	1 cut(s)	6
TaaI	ACNGT	2 cut(s)	369, 397
TaiI	ACGT	3 cut(s)	36, 440, 689
TaqI	TCGA	1 cut(s)	364
TasI	AATT	6 cut(s)	228, 282, 312, 454, 789, 1307
TatI	WGTACW	3 cut(s)	334, 785, 872
TauI	GCSGC	1 cut(s)	1238
TfiI	GAWTC	3 cut(s)	597, 823, 1271
Tru1I	TTAA	6 cut(s)	192, 414, 792, 855, 1256, 1310
Tru9I	TTAA	6 cut(s)	192, 414, 792, 855, 1256, 1310
TscAI	CASTG	5 cut(s)	106, 400, 854, 898, 916
TseFI	GTSAC	1 cut(s)	320
TseI	GCWGC	1 cut(s)	20
Tsp45I	GTSAC	1 cut(s)	320
TspDTI	ATGAA	4 cut(s)	915, 956, 989, 1319
TspGWI	ACGGA	1 cut(s)	65
TspRI	CASTG	5 cut(s)	106, 400, 854, 898, 916
Van91I	CCANNNNNTGG	1 cut(s)	572
VpaK11BI	GGWCC	2 cut(s)	52, 409
XapI	RAATTY	2 cut(s)	228, 282
XmiI	GTMKAC	2 cut(s)	364, 634
XmnI	GAANNNNTTC	1 cut(s)	882
XspI	CTAG	1 cut(s)	1263
ZrmI	AGTACT	1 cut(s)	874

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.