Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0011105

Physical Location & Seq

Genomic Coordinates Reverse (-)

40551890 .. 40558423

Gene Structure 6534 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000029568

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGCCATGGAAGACGAAGCAGCGGAGAAGAACGTGATGTGTCTTCTGACGGACTCAGAAGATACTCCTTTAGGAAAGTCATTGTATCTTCCTCAACACACTGGCACCCAGCAACTTCAACAAATCGTCAACCAGCTTCTGAACAATGTGGATAAGGTGCCTTATACTTTCTATATAGCAGATAAGGAACTTAATCAGTCGCTTGGCGAATATGCAGAGAAGAGTAAAAGTCACTCAGAAGCTGTACTTTCAGTTGCTTTTAGTCCTGATGGTCGACAGTTGGCGAGTGGTTCTGGTGATACCAATGTCCGACTATGGGACCTTAATACTCAGACACCTTTGCACACGTGTTCAGGACATAAGAATTGGGTTCTTTGTATTGCATGGTCTGCTGATGGCAAGTATCTTGTTAGTGGGAGCAAGTCTGGAGAACTTCAATGTTGGGATCCACAGACAGGAAAACCAGCAGGCAATCCACTTACTGGTCACAAGAAATGGATTACTGGAATCTCTTGGGAACCAGTCCACCTTAGTGCTCCATGTGGACGTTTTGTAAGTGCTGGGAAAGATGGTGATGCACGCATATGGGACATCACGTTGAAGAAATGTGTTATATGTCTTAGTGGCCACACACTTGCAATAACTTGTGTAAAATGGGGTGGAGATGGTTTTATATATACAGGTTCCCAAGATTGTACAATTAAGGTCTGGGAGACTACACAGGGGAAGCTGATTCGTGAGCTAAAGGGTCATGGGCACTGGGTTAACTCTCTTGCATTGAGTACTGAATATGTTCTTCGCACTGGAGCTTATGATCACACTGGCAAGCAATATTCATCTCCTGAGGAAATGAAAAAGGTAGCTTTGGAAAGGTATAACAAAATGAAAGGTAAAGCTCCTGAACGATTGGTTTCTGGATCTGATGACTTTACTATGTTTCTATGGGAGCCTTTTGTCAGCAAACAGCCCAAAGCTCGCATGACAGGTCATCAACAGCTTGTAAACCATGTCTGCTTTTCACCTGATGGGCAATGGATAGCAAGCGCCTCATTTGATAGGTCTGTCAGGATATGGAATGGTACTACTGGGAAGTTTTTTGGAGTTCTCCGGGGCCATGTTGGTGCTGTTTACCAGATCAGCTGGGCCGCAGACAGCAGGCTACTTTTAAGTGCTAGCAAAGATTCAACTCTGAAGGTTTGGGATATCAAGACTATGAAATTAAAACAAGACCTTCCAGGTCATGCAGATGAGGTTTATGCTGTTGATTGGAGTCCAGATGGTGAAAAGGTTGCTTCTGGTGGTAAGGATAGAGTTTTGAAGTTATGGATGGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000027 GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005840 GO:0006996 GO:0007275 GO:0008150 GO:0009553 GO:0009987 GO:0016043 GO:0022607 GO:0022613 GO:0022618 GO:0031461 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0042254 GO:0042255 GO:0042273 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0065003 GO:0070013 GO:0070925 GO:0071826 GO:0071840 GO:0080008 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NLE WD40

Protein Analysis

445

Amino Acids

49.16

Weight (kDa)

7.95

Isoelectric Point (pI)

26.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NLE	PF08154	12 - 71	3.8e-13	NLE (NUC135) domain
WD40_MABP1-WDR62_2nd	PF24782	48 - 251	1.7e-10	MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	59 - 204	1.8e-11	CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2	PF23414	69 - 159	9.1e-16	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	69 - 204	6.7e-25	WDR3 first beta-propeller domain
Beta-prop_IFT140_1st	PF23383	70 - 163	3.2e-07	IFT140 first beta-propeller
WD40_CDC20-Fz	PF24807	71 - 130	3e-11	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	72 - 116	9.3e-07	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	73 - 124	1.4e-10	THOC3 beta-propeller domain
Beta-prop_WDR19_1st	PF23389	73 - 173	9.4e-08	WDR19 first beta-propeller
WDR55	PF24796	73 - 156	4.4e-09	WDR55
Beta-prop_TEP1_2nd	PF25047	74 - 169	8.5e-16	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	75 - 119	6e-07	WDHD1 first WD40 domain
ANAPC4_WD40	PF12894	75 - 130	8.3e-07	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR5	PF25175	76 - 223	2.4e-29	WDR5 beta-propeller domain
Beta-prop_Aladin	PF25460	76 - 152	5.2e-07	Aladin seven-bladed propeller
WD40	PF00400	76 - 107	2.7e-09	WD domain, G-beta repeat
Beta-prop_EML	PF23409	77 - 286	5.4e-12	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	77 - 150	7.1e-06	WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd	PF25172	78 - 243	2.5e-17	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	80 - 139	8.3e-08	MABP1/WDR62 first WD40 domain
Beta-prop_IFT122_1st	PF23381	81 - 223	9.5e-08	IFT122 first beta-propeller
WD40_Prp19	PF24814	111 - 274	2.1e-19	Prp19 WD40 domain
WD40	PF00400	112 - 149	6.7e-10	WD domain, G-beta repeat
WD40_Gbeta	PF25391	116 - 284	1.3e-11	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	119 - 238	7.4e-13	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	119 - 175	3.5e-07	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	120 - 223	7.9e-17	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	121 - 178	1.4e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	160 - 278	2.2e-16	WDR3 first beta-propeller domain
Beta-prop_SCAP	PF24017	176 - 272	1.5e-06	SCAP Beta-propeller
Beta-prop_WDR5	PF25175	180 - 274	4.9e-16	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	182 - 269	1.3e-06	TEP-1 second beta-propeller
WDR55	PF24796	182 - 404	7.3e-16	WDR55
Beta-prop_CAF1B_HIR1	PF24105	183 - 364	2.6e-09	CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz	PF24807	185 - 276	2.6e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd	PF25172	186 - 282	5.7e-06	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	192 - 262	3.2e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	202 - 237	3e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	210 - 275	3.9e-07	THOC3 beta-propeller domain
WD40_Prp19	PF24814	296 - 442	8.4e-22	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	300 - 404	8.4e-11	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	301 - 364	3e-07	WDHD1 first WD40 domain
Beta-prop_TEP1_2nd	PF25047	301 - 442	8e-16	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	301 - 409	3.1e-20	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	301 - 442	1.3e-29	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	302 - 372	1.1e-13	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	322 - 407	1.8e-11	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	322 - 359	1.5e-11	WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd	PF25168	324 - 365	5.3e-07	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	326 - 442	6.8e-19	CDC20/Fizzy WD40 domain
EIF3I	PF24805	327 - 406	3.3e-13	EIF3I
Beta-prop_WDR5	PF25175	327 - 442	3.1e-32	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	327 - 442	3.5e-19	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	328 - 438	1.4e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3	PF25174	330 - 442	2e-27	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	334 - 401	5.7e-07	MABP1/WDR62 second WD40 domain
WDR90_beta-prop_4th	PF23342	334 - 438	1.4e-08	WDR90, 4th beta-propeller
WD40	PF00400	364 - 401	3e-08	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	367 - 441	2.9e-12	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	368 - 436	2.4e-07	WDR36/Utp21 second beta-propeller domain
WD40_Gbeta	PF25391	368 - 440	1.4e-11	G protein beta WD-40 repeat protein
Beta-prop_Aladin	PF25460	374 - 442	1.2e-07	Aladin seven-bladed propeller
WD40_WDHD1_1st	PF24817	374 - 442	1e-06	WDHD1 first WD40 domain
WD40	PF00400	406 - 442	8e-09	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

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Orthologous Genes (Group: OG0011105)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52820
fragaria_vesca	FvH4_7g01500
malus_domestica	MD02G1305600.v1.1 MD09G1280600.v1.1 MD17G1287700.v1.1
prunus_persica	Prupe.2G020500_v2.0.a1
pyrus_communis	pycom02g25610 pycom17g27070
rosa_chinensis	RchiOBHm_Chr1g0317321
rosa_laevigata	RLG00000029568 RLG00000030606
rosa_multiflora	Rmu_sc0005092.1_g000002 Rmu_sc0013869.1_g000006
rosa_roxburghii	Rroxscaffold_4G00330490
rosa_rugosa	Rorug01G0015900
rosa_samantha	Rh1AG027300 Rh1BG023200 Rh1CG025300 Rh1DG045900
rosa_wichuraiana	Rw1G001910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	104, 157
AccB7I	CCANNNNNTGG	1 cut(s)	482
AccI	GTMKAC	1 cut(s)	274
AciI	CCGC	2 cut(s)	23, 1146
AclWI	GGATC	3 cut(s)	440, 453, 925
AcoI	YGGCCR	2 cut(s)	3, 625
AcuI	CTGAAG	1 cut(s)	1211
AcvI	CACGTG	1 cut(s)	348
AfaI	GTAC	4 cut(s)	246, 697, 784, 1081
AfiI	CCNNNNNNNGG	3 cut(s)	316, 455, 482
AflIII	ACRYGT	2 cut(s)	345, 347
AgsI	TTSAA	5 cut(s)	119, 437, 601, 1185, 1318
AjnI	CCWGG	1 cut(s)	1234
AleI	CACNNNNGTG	1 cut(s)	531
Alw21I	GWGCWC	1 cut(s)	538
Alw26I	GTCTC	1 cut(s)	707
AlwI	GGATC	3 cut(s)	440, 453, 925
AlwNI	CAGNNNCTG	2 cut(s)	139, 242
AoxI	GGCC	4 cut(s)	3, 625, 1111, 1143
ApeKI	GCWGC	1 cut(s)	20
Asp700I	GAANNNNTTC	1 cut(s)	792
AspLEI	GCGC	1 cut(s)	1046
AspS9I	GGNCC	3 cut(s)	319, 1111, 1143
AsuC2I	CCSGG	1 cut(s)	1109
AsuHPI	GGTGA	4 cut(s)	308, 584, 1011, 1292
AsuNHI	GCTAGC	1 cut(s)	1172
AvaII	GGWCC	1 cut(s)	319
AxyI	CCTNAGG	1 cut(s)	843
BaeGI	GKGCMC	1 cut(s)	759
BaeI	ACNNNNGTAYC	2 cut(s)	291, 324
BalI	TGGCCA	2 cut(s)	5, 627
BamHI	GGATCC	1 cut(s)	445
BanI	GGYRCC	2 cut(s)	104, 157
BbrPI	CACGTG	1 cut(s)	348
BbsI	GAAGAC	2 cut(s)	18, 35
Bbv12I	GWGCWC	1 cut(s)	538
BbvI	GCAGC	1 cut(s)	32
BccI	CCATC	7 cut(s)	263, 389, 563, 659, 1019, 1271, 1321
BciT130I	CCWGG	1 cut(s)	1236
BclI	TGATCA	1 cut(s)	814
BcnI	CCSGG	1 cut(s)	1109
BcoDI	GTCTC	1 cut(s)	707
BfaI	CTAG	1 cut(s)	1173
BfoI	RGCGCY	1 cut(s)	1047
BisI	GCNGC	2 cut(s)	21, 1146
BlsI	GCNGC	2 cut(s)	22, 1147
BmcAI	AGTACT	1 cut(s)	784
Bme1390I	CCNGG	2 cut(s)	1109, 1236
Bme18I	GGWCC	1 cut(s)	319
BmgT120I	GGNCC	3 cut(s)	319, 1111, 1143
BmiI	GGNNCC	8 cut(s)	106, 159, 320, 447, 519, 685, 948, 1112
BmrFI	CCNGG	2 cut(s)	1109, 1236
BmrI	ACTGGG	2 cut(s)	769, 1095
BmsI	GCATC	1 cut(s)	565
BmtI	GCTAGC	1 cut(s)	1176
BmuI	ACTGGG	2 cut(s)	769, 1095
BpiI	GAAGAC	2 cut(s)	18, 35
BpmI	CTGGAG	2 cut(s)	447, 825
BpuMI	CCSGG	1 cut(s)	1109
BsaAI	YACGTR	1 cut(s)	348
BsaJI	CCNNGG	2 cut(s)	6, 1108
Bsc4I	CCNNNNNNNGG	3 cut(s)	316, 455, 482
Bse1I	ACTGG	8 cut(s)	106, 487, 508, 521, 764, 808, 826, 1090
Bse21I	CCTNAGG	1 cut(s)	843
Bse3DI	GCAATG	1 cut(s)	1037
BseBI	CCWGG	1 cut(s)	1236
BseDI	CCNNGG	2 cut(s)	6, 1108
BseGI	GGATG	1 cut(s)	1332
BseLI	CCNNNNNNNGG	3 cut(s)	316, 455, 482
BseMI	GCAATG	1 cut(s)	1037
BseMII	CTCAG	4 cut(s)	69, 249, 344, 834
BseNI	ACTGG	8 cut(s)	106, 487, 508, 521, 764, 808, 826, 1090
BseSI	GKGCMC	1 cut(s)	759
BseXI	GCAGC	1 cut(s)	32
BseYI	CCCAGC	3 cut(s)	108, 560, 1140
BshFI	GGCC	4 cut(s)	5, 627, 1113, 1145
BshNI	GGYRCC	2 cut(s)	104, 157
BsiHKAI	GWGCWC	1 cut(s)	538
BsiSI	CCGG	1 cut(s)	1108
BslFI	GGGAC	2 cut(s)	332, 602
BslI	CCNNNNNNNGG	3 cut(s)	316, 455, 482
BsmAI	GTCTC	1 cut(s)	707
BsmFI	GGGAC	2 cut(s)	332, 602
BsnI	GGCC	4 cut(s)	5, 627, 1113, 1145
Bsp1286I	GDGCHC	2 cut(s)	538, 759
Bsp1407I	TGTACA	1 cut(s)	695
Bsp143I	GATC	4 cut(s)	445, 814, 917, 1134
Bsp19I	CCATGG	1 cut(s)	6
BspACI	CCGC	2 cut(s)	23, 1146
BspANI	GGCC	4 cut(s)	5, 627, 1113, 1145
BspCNI	CTCAG	4 cut(s)	68, 248, 343, 835
BspLI	GGNNCC	8 cut(s)	106, 159, 320, 447, 519, 685, 948, 1112
BspOI	GCTAGC	1 cut(s)	1176
BspPI	GGATC	3 cut(s)	440, 453, 925
BspT107I	GGYRCC	2 cut(s)	104, 157
BsrDI	GCAATG	1 cut(s)	1037
BsrGI	TGTACA	1 cut(s)	695
BsrI	ACTGG	8 cut(s)	106, 487, 508, 521, 764, 808, 826, 1090
BssECI	CCNNGG	2 cut(s)	6, 1108
BssMI	GATC	4 cut(s)	445, 814, 917, 1134
BssT1I	CCWWGG	1 cut(s)	6
Bst2UI	CCWGG	1 cut(s)	1236
Bst4CI	ACNGT	1 cut(s)	279
Bst6I	CTCTTC	1 cut(s)	215
BstAPI	GCANNNNNTGC	1 cut(s)	389
BstAUI	TGTACA	1 cut(s)	695
BstBAI	YACGTR	1 cut(s)	348
BstC8I	GCNNGC	7 cut(s)	469, 580, 827, 976, 1042, 1157, 1174
BstDEI	CTNAG	6 cut(s)	55, 235, 330, 530, 620, 843
BstDSI	CCRYGG	1 cut(s)	6
BstF5I	GGATG	1 cut(s)	1332
BstH2I	RGCGCY	1 cut(s)	1047
BstHHI	GCGC	1 cut(s)	1046
BstKTI	GATC	4 cut(s)	448, 817, 920, 1137
BstMAI	GTCTC	1 cut(s)	707
BstMBI	GATC	4 cut(s)	445, 814, 917, 1134
BstMWI	GCNNNNNNNGC	1 cut(s)	389
BstNI	CCWGG	1 cut(s)	1236
BstSCI	CCNGG	2 cut(s)	1107, 1234
BstSLI	GKGCMC	1 cut(s)	759
BstV1I	GCAGC	1 cut(s)	32
BstV2I	GAAGAC	2 cut(s)	18, 35
BstX2I	RGATCY	2 cut(s)	445, 917
BstYI	RGATCY	2 cut(s)	445, 917
Bsu36I	CCTNAGG	1 cut(s)	843
BsuRI	GGCC	4 cut(s)	5, 627, 1113, 1145
BtgI	CCRYGG	1 cut(s)	6
BtsCI	GGATG	1 cut(s)	1332
BtsIMutI	CAGTG	4 cut(s)	99, 757, 801, 819
Cac8I	GCNNGC	7 cut(s)	469, 580, 827, 976, 1042, 1157, 1174
CaiI	CAGNNNCTG	2 cut(s)	139, 242
CfoI	GCGC	1 cut(s)	1046
Cfr13I	GGNCC	3 cut(s)	319, 1111, 1143
Csp6I	GTAC	4 cut(s)	245, 696, 783, 1080
CviAII	CATG	8 cut(s)	7, 384, 540, 752, 979, 1007, 1115, 1241
CviQI	GTAC	4 cut(s)	245, 696, 783, 1080
DdeI	CTNAG	6 cut(s)	55, 235, 330, 530, 620, 843
DpnI	GATC	4 cut(s)	447, 816, 919, 1136
DpnII	GATC	4 cut(s)	445, 814, 917, 1134
EaeI	YGGCCR	2 cut(s)	3, 625
Eam1104I	CTCTTC	1 cut(s)	215
EarI	CTCTTC	1 cut(s)	215
Eco130I	CCWWGG	1 cut(s)	6
Eco32I	GATATC	1 cut(s)	1204
Eco47I	GGWCC	1 cut(s)	319
Eco57I	CTGAAG	1 cut(s)	1211
Eco72I	CACGTG	1 cut(s)	348
Eco81I	CCTNAGG	1 cut(s)	843
EcoO109I	RGGNCCY	1 cut(s)	319
EcoRII	CCWGG	1 cut(s)	1234
EcoRV	GATATC	1 cut(s)	1204
EcoT14I	CCWWGG	1 cut(s)	6
ErhI	CCWWGG	1 cut(s)	6
FaeI	CATG	8 cut(s)	10, 387, 543, 755, 982, 1010, 1118, 1244
FalI	AAGNNNNNCTT	2 cut(s)	231, 263
FaqI	GGGAC	2 cut(s)	332, 602
FatI	CATG	8 cut(s)	6, 383, 539, 751, 978, 1006, 1114, 1240
FauNDI	CATATG	1 cut(s)	584
FbaI	TGATCA	1 cut(s)	814
FblI	GTMKAC	1 cut(s)	274
Fnu4HI	GCNGC	2 cut(s)	21, 1146
Fsp4HI	GCNGC	2 cut(s)	21, 1146
FspBI	CTAG	1 cut(s)	1173
GlaI	GCGC	1 cut(s)	1045
GluI	GCNGC	2 cut(s)	21, 1146
GsaI	CCCAGC	3 cut(s)	112, 564, 1144
GsuI	CTGGAG	2 cut(s)	447, 825
HaeII	RGCGCY	1 cut(s)	1047
HaeIII	GGCC	4 cut(s)	5, 627, 1113, 1145
HapII	CCGG	1 cut(s)	1108
HhaI	GCGC	1 cut(s)	1046
Hin1II	CATG	8 cut(s)	10, 387, 543, 755, 982, 1010, 1118, 1244
Hin6I	GCGC	1 cut(s)	1044
HinP1I	GCGC	1 cut(s)	1044
HincII	GTYRAC	3 cut(s)	130, 275, 766
HindII	GTYRAC	3 cut(s)	130, 275, 766
HinfI	GANTC	5 cut(s)	53, 507, 733, 1181, 1270
HpaI	GTTAAC	1 cut(s)	766
HpaII	CCGG	1 cut(s)	1108
HphI	GGTGA	4 cut(s)	308, 584, 1011, 1292
Hpy166II	GTNNAC	7 cut(s)	130, 275, 526, 545, 766, 1003, 1129
Hpy188I	TCNGA	8 cut(s)	48, 58, 141, 238, 311, 333, 922, 1191
Hpy8I	GTNNAC	7 cut(s)	130, 275, 526, 545, 766, 1003, 1129
HpyAV	CCTTC	2 cut(s)	1186, 1241
HpyCH4III	ACNGT	1 cut(s)	279
HpyCH4IV	ACGT	4 cut(s)	33, 347, 547, 596
HpyCH4V	TGCA	7 cut(s)	215, 343, 383, 578, 638, 776, 1244
HpyF10VI	GCNNNNNNNGC	1 cut(s)	389
HpyF3I	CTNAG	6 cut(s)	55, 235, 330, 530, 620, 843
HpySE526I	ACGT	4 cut(s)	33, 347, 547, 596
Hsp92II	CATG	8 cut(s)	10, 387, 543, 755, 982, 1010, 1118, 1244
HspAI	GCGC	1 cut(s)	1044
Ksp22I	TGATCA	1 cut(s)	814
KspAI	GTTAAC	1 cut(s)	766
Kzo9I	GATC	4 cut(s)	445, 814, 917, 1134
LmnI	GCTCC	5 cut(s)	417, 541, 806, 901, 946
Lsp1109I	GCAGC	1 cut(s)	32
LweI	GCATC	1 cut(s)	565
MaeI	CTAG	1 cut(s)	1173
MaeII	ACGT	4 cut(s)	33, 347, 547, 596
MaeIII	GTNAC	2 cut(s)	230, 485
MalI	GATC	4 cut(s)	447, 816, 919, 1136
MboI	GATC	4 cut(s)	445, 814, 917, 1134
MboII	GAAGA	8 cut(s)	23, 35, 40, 71, 80, 232, 613, 788
MflI	RGATCY	2 cut(s)	445, 917
MhlI	GDGCHC	2 cut(s)	538, 759
MlsI	TGGCCA	2 cut(s)	5, 627
MluCI	AATT	3 cut(s)	364, 699, 1217
MluNI	TGGCCA	2 cut(s)	5, 627
MlyI	GAGTC	2 cut(s)	47, 1279
MmeI	TCCRAC	1 cut(s)	334
MnlI	CCTC	4 cut(s)	102, 838, 1057, 1243
Mox20I	TGGCCA	2 cut(s)	5, 627
MroXI	GAANNNNTTC	1 cut(s)	792
MscI	TGGCCA	2 cut(s)	5, 627
MseI	TTAA	6 cut(s)	192, 324, 702, 765, 1166, 1220
MslI	CAYNNNNRTG	4 cut(s)	531, 583, 1119, 1245
Msp20I	TGGCCA	2 cut(s)	5, 627
MspA1I	CMGCKG	2 cut(s)	23, 1140
MspI	CCGG	1 cut(s)	1108
MspR9I	CCNGG	2 cut(s)	1109, 1236
MvaI	CCWGG	1 cut(s)	1236
MwoI	GCNNNNNNNGC	1 cut(s)	389
NciI	CCSGG	1 cut(s)	1109
NcoI	CCATGG	1 cut(s)	6
NdeI	CATATG	1 cut(s)	584
NdeII	GATC	4 cut(s)	445, 814, 917, 1134
NheI	GCTAGC	1 cut(s)	1172
NlaIII	CATG	8 cut(s)	10, 387, 543, 755, 982, 1010, 1118, 1244
NlaIV	GGNNCC	8 cut(s)	106, 159, 320, 447, 519, 685, 948, 1112
NmuCI	GTSAC	2 cut(s)	230, 485
OliI	CACNNNNGTG	1 cut(s)	531
PdmI	GAANNNNTTC	1 cut(s)	792
PfeI	GAWTC	3 cut(s)	507, 733, 1181
PflMI	CCANNNNNTGG	1 cut(s)	482
PkrI	GCNGC	2 cut(s)	22, 1147
PleI	GAGTC	2 cut(s)	47, 1278
PmaCI	CACGTG	1 cut(s)	348
PmlI	CACGTG	1 cut(s)	348
PpsI	GAGTC	2 cut(s)	47, 1278
Ppu21I	YACGTR	1 cut(s)	348
PpuMI	RGGWCCY	1 cut(s)	319
Psp5II	RGGWCCY	1 cut(s)	319
Psp6I	CCWGG	1 cut(s)	1234
PspCI	CACGTG	1 cut(s)	348
PspFI	CCCAGC	3 cut(s)	108, 560, 1140
PspGI	CCWGG	1 cut(s)	1234
PspN4I	GGNNCC	8 cut(s)	106, 159, 320, 447, 519, 685, 948, 1112
PspPI	GGNCC	3 cut(s)	319, 1111, 1143
PspPPI	RGGWCCY	1 cut(s)	319
PstNI	CAGNNNCTG	2 cut(s)	139, 242
PsuI	RGATCY	2 cut(s)	445, 917
PvuII	CAGCTG	1 cut(s)	1140
RsaI	GTAC	4 cut(s)	246, 697, 784, 1081
RsaNI	GTAC	4 cut(s)	245, 696, 783, 1080
RseI	CAYNNNNRTG	4 cut(s)	531, 583, 1119, 1245
SalI	GTCGAC	1 cut(s)	273
SaqAI	TTAA	6 cut(s)	192, 324, 702, 765, 1166, 1220
SatI	GCNGC	2 cut(s)	21, 1146
Sau3AI	GATC	4 cut(s)	445, 814, 917, 1134
Sau96I	GGNCC	3 cut(s)	319, 1111, 1143
ScaI	AGTACT	1 cut(s)	784
SchI	GAGTC	2 cut(s)	47, 1279
ScrFI	CCNGG	2 cut(s)	1109, 1236
SduI	GDGCHC	2 cut(s)	538, 759
SfaNI	GCATC	1 cut(s)	565
SinI	GGWCC	1 cut(s)	319
SmiMI	CAYNNNNRTG	4 cut(s)	531, 583, 1119, 1245
Sse9I	AATT	3 cut(s)	364, 699, 1217
SsiI	CCGC	2 cut(s)	23, 1146
SspI	AATATT	1 cut(s)	833
SspMI	CTAG	1 cut(s)	1173
StyD4I	CCNGG	2 cut(s)	1107, 1234
StyI	CCWWGG	1 cut(s)	6
TaaI	ACNGT	1 cut(s)	279
TaiI	ACGT	4 cut(s)	36, 350, 550, 599
TaqI	TCGA	1 cut(s)	274
TasI	AATT	3 cut(s)	364, 699, 1217
TatI	WGTACW	3 cut(s)	244, 695, 782
TauI	GCSGC	1 cut(s)	1148
TfiI	GAWTC	3 cut(s)	507, 733, 1181
Tru1I	TTAA	6 cut(s)	192, 324, 702, 765, 1166, 1220
Tru9I	TTAA	6 cut(s)	192, 324, 702, 765, 1166, 1220
TscAI	CASTG	4 cut(s)	106, 764, 808, 826
TseFI	GTSAC	2 cut(s)	230, 485
TseI	GCWGC	1 cut(s)	20
Tsp45I	GTSAC	2 cut(s)	230, 485
TspDTI	ATGAA	4 cut(s)	825, 866, 899, 1229
TspGWI	ACGGA	1 cut(s)	65
TspRI	CASTG	4 cut(s)	106, 764, 808, 826
Van91I	CCANNNNNTGG	1 cut(s)	482
VpaK11BI	GGWCC	1 cut(s)	319
XmiI	GTMKAC	1 cut(s)	274
XmnI	GAANNNNTTC	1 cut(s)	792
XspI	CTAG	1 cut(s)	1173
ZrmI	AGTACT	1 cut(s)	784

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.