FvH4_7g02390

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008765
Physical Location & Seq
Forward (+)
2676057 .. 2686722
10666 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02390.t2

Sequence Viewer

Length: 4566 bp
ATGACGAGGGTCTACGGCACCGGCGCGTACGATTTCAAGCGTCACCACGTCGCCGAGTACCCGGTTGGCGACAAGCCGGTCGAGGCCAAGCCCGGCGCCGCGCTCCCGAGCTCGATTACCTTGTCGGAGATTCAGCGCGATCAGCTCACCGTGATTGCCGCCGCGAACTGGTCCAGGGTCGGCGACGCCAAGGAGAAGAAGCCGGCGTTTGATCCGGAGCTGGTGAAGAGGATTTACGAGACGGAGCTTCGTGTGAAGGAGGGGGAGAGGAAGACGGTGCCGTTGCAGAGAGTCATGATTTTGGAGGTCAGCCAGTACTTGGAGAACTATCTGTTCCCGAATTTTGATGCCGAAACGGCCACGTTTGAGCATGTTATGTCTATGATTCTTATGGTCAATGAGAAGTTTCGAGAGAATGTGGCTGCTTGGGTGTGCTTTTATGACCGGAAAGATGCGTTTAAGGGATTCCTTGGAAGGGTTCTTGGGCTTAAATCGGAAAGAGAATTAGGTATAGCAGAGAAGACAAATTATCTAGTATTCATGATAAATGCCTTCCAGAGTTTGGAAGATGAGATAGTCAGTGAGACTGTCCTTAGATTGGCAAGTTTCCAATCCTGGCACAGTTTGTCGTATGGTCGTTTTCAGATGGAGCTTGGTTTAAATTCAGATTTGATCAAGAAATGGAGAAGGATGGTAAAGAGGGAAGCTGCAAAACATGGAGAGTCTTTTAACCCATCAACTGCACTTGAAGTACAGTTCTTAAGAAATCTCATTGAAGAGTTCCTAGAGATACTTGATTCCAAAGTCTTACGGCCAAATCATGGTGTCAACGGAGAGGATCAACTTCTTGATGTTAATGGAATGGAACACGTTGATGATGCTTGTGTTTTGTACTGTGAGAGGTTTGTGGAATTTCTTATTGACCTCTTGAGCCAGCTACCAACAAGGAGGTACTTGAGGCCACTTGTAGCGGATGTTGCTGTTGTTCCCAAATGCCACTTGAGTGCTTTGTATAGACATGAAAAGGGGAAGCTCTTTACCCAACTTGTCGACTTGCTACAATTTTATGAAGGGTTTGAGATAAATGATAATGTTGGGAAACAATTGACTGATGATGAAGTGCTCCAATCTCATTATGATCGCGTACAATCTTTCCAGCTGCTTGCTTTTAAAAAGATTCCTAAGTTGAAAGAGCTTGCATTGGCTAACATTGGTTCAATTGACAACCGAAATGATCTTACCAAGAGATTGTCTGTACTTTCTCCTGAAGAATTGAAGGATTTGGTTTGCTCAAAGTTGAAGCTGATTTCTAAGAATGATCCTTGGTCATCCAGGGTTGATTTCCTTACTGAAGTGATGGTTTCCTTTTTCAAGAGGCAACAGTCTCAAAAAGAGAAAATAAATGCTCTTCCACTGTATCCAAATGAGCTGATCATGTGGGATGAAAGCCTTGTGCCAAGCATTAATTACTCTGGAGAAGGTTGTCTGGCTCTTCCAAAACTTAATCTACAATTCTTGACGCTGCATGATTATCTCCTGAGAAATTTTGATCTCTTCCGTCTTGAATCAACATATGAGATTCGTGAGGATATTCAAGAGGCTGTGCCACATCTTAATGCCTGCATTAATAATGAGGGAGAAACTGTTTTCCGTGGTTGGTCAAGGATGGCTGTGCCGATTAAAGAATTTAAGATCAGTGAGGTAAAACAGCCAAATATTGGAGAAGTTAAGCCAGCTGCTGTGACAGCTGAAATCACTTATAGTATATCCAGTTATAAAGCACAGGTGCGATCAGAATGGAATGCTCTTAAAGAGCATGATGTTCTATTTTTACTTTCTATACGCCCTTCCTTTGAGCCTCTAAGCGCAGAAGAGGGTGCTAAGGCTAGTGTGCCTCAGAAGCTTGGTCTTCAATATGTACGGGGTTGTGAAATCATTGAGGTTCGTGATGAGGAAGGAACTCTAATGAATGATTTCACTGGAAGAATTAAACGGGATGAGTGGAAGCCTCCAAAAGGAGAATTGAGAACTTTGACTGTTGCTTTGGATACTGCACAATACTACATGGATGTCAGCAACACTGCTGCAAAGGGTGCAGAAGATGTTTATGGGACATTCAATATTTTGATGAGAAGAAAGCCCAAGGAAAATAATTTCAAAGCAATTTTAGAATCTATTAGAGATCTTATGAATGAATATTGTATTGTTCCTGACTGGTTGCACAAAACATTTCTGGGGTATGGTAATCCTTCTGCCGCACAATGGACTAATATGCCAGATCTCTTAGAAACAGTAGATTTTAAGGATACTTTCCTTGATGCGGACCATCTGAGGGAGTGTTTTCCAGACTATCAGGTATTCTTTGTGAGTCCAGATGGTACAGAAAATATGGATCCAAGGCCTCCTTTTCGAGTAAGGCTTCCCAAGACGATCAAAAGCAGCACTAATGCTCTTGCAGGCAATAAGAAAGCAAAGATGAGTTCTATGAGTGATGTCCCCATTGAGGACTCTGATAAGGGGAACGAAAAATTTGTTGTTGAAGCATACACCCCTCCTGACCCAGGTCCATATCCCCAAGACCAGCCAAGGCAGAACTCAGTTAGGTTTACACCCACTCAGGTTGGAGCAATCCTCTCAGGTATTCAGCCAGGACTAACAATGGTTGTGGGTCCACCTGGTACGGGAAAGACTGATACAGCTGTGCAAATTCTGAATGTTCTGTATCATAATTGTCCATCTCAGAGAACTTTGATAATAACTCACTCGAACCAGGCTCTGAATGACCTTTTTGAAAAGATAATGCAGAGGGATGTACCTGCACGCTATCTTCTTCGACTTGGGCAAGGAGAACAAGAGCTAGCAACTGATCTTGACTTCAGTCGGCAAGGCCGTGTCAATGCCATGCTTGTGAGGCGGTTGGAGTTGCTTAGTGAAGTGGAGAGGCTGGCAAGATCTCTCCAATTGCCTGAGGATGTTGGTTATACATGTGAAACTGCTGGATACTTTTGGTTGCTTCATGTATACTCGCGCTGGGAGCTATTCCTGGCTGCTTGTAACGAAAATAAAAATAAACAGTCATTTGTTAAAGATCGATTTCCTTTCAAGGAATTCTTCTCCGACTCTCCAAAGCCGGTTTTTACTGGTGAGTCATTCGAGAAAGATATGCGAGCAGCTAAGGGTTGCTTTCGTCATCTAAAGACTGTGTTTCAGGAGCTTGAAGAGTGTAGGGCTTTTGAGTTACTGAAGTCTACAGCTGATCGAGCAAACTACTTGATGACTAAGCAGGCAAAGATAGTAGCCATGACATGCACTCACGCAGCTCTGAAGAGGAAGGATTTCCTCCAGTTAGGTTTCAAGTATGATAACTTGCTAATGGAAGAAAGTGCTCAAATTTTGGAGATCGAAACTTTTATTCCAATGTTGCTCCAGAGGCAAGAAGATGGTTATGCACGGCTTAAACGTTGCATCCTGATTGGTGATCATCACCAGTTGCCTCCTGTTGTCAAGAATATGGCCTTTCAAAAGTATAGCCATATGGACCAGAGTCTCTTTACAAGGTTTGTTCGTTTGGGCATTCCTTACATTGAACTTAATGCCCAAGGTAGGGCCAGGCCAAGTATAGCCAAACTTTACAACTGGAGGTATAGAGAATTGGGAGACCTTCCCTATGTAAAGCAAGATGCCATTTTCAAAAGAGCAAATTCTGGATTCTCCTTTGAATATCAGTTGGTGGATGTACCAGATTACCATGACAGAGGTGAGTCTGCCCCATCACCTTGGTTCTACCAAAATGAGGGAGAAGCTGAATATGTTGTCAGTGTGTATATTTACATGCGTCTACTTGGGTATCCTGCAAACAAGATATCAATATTGACCACTTATAATGGCCAGAAGCTTTTAATCCGTGATGTTATTAACAGACGATGTGCTCCGTACGACTTCATAGGCCCTCCCAGCAAGGTGACCACAGTTGACAAATTTCAGGGTCAGCAGAATGATTTTATATTGCTCTCTCTGGTGCGTACTCGCTTTGTGGGTCACCTTCGTGATGTTCGGAGATTGATTGTTGCCATGTCTCGTGCCCGATTGGGTTTGTATGTGTTTTGCCGCCGTTCTTTGTTTGAGCAATGCTATGAACTTCAGCCTACATTCCAACATTTACTTCAGAGACCTGATTGCCTTGCTCTTAATTTTAATGAGACTACACCACACACAGAACGCCATGTTGAAGAAACGGGGCCTGTCCATCTTGTAAGCAGTGTTGATGAGATGATCAGTATCTACCAGCAGCTCTATGCGGTGAAGTTTCATCAGTATGTGGCTCCTTCAATTCTGCAAACATCTATGTCTGGGCAGGATCCTATGGATGCAGATATCCCTGTATCTGCTGATGGGGTGCCAGATGATACACCGCATGTAAGCAATTCGGAACTGGAGGACAACGGCCGAAAAGTGGATTCGTCAGTTGAGAATCATTCAAATGGGGTGACAGAAATGGAAACTAGTCTGGATGATGCTCATGTGGTTCCCGAAAGCAATTCAGACCAAGTGAGAATGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

1522

Amino Acids

174.4

Weight (kDa)

5.79

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 49 - 461 5.1e-134 Intron-binding protein aquarius N-terminal
Aquarius_N_2nd PF21143 543 - 712 1.4e-56 Intron-binding protein aquarius, beta-barrel
Aquarius_N_3rd PF21144 762 - 861 4.3e-21 Intron-binding protein aquarius insert domain
AAA_11 PF13086 874 - 1170 2e-28 AAA domain
AAA_12 PF13087 1179 - 1371 5.1e-27 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1776, 3882
AasI GACNNNNNNGTC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 2824
AccB1I GGYRCC 4 cut(s) 17, 95, 277, 4396
AccB7I CCANNNNNTGG 4 cut(s) 319, 562, 821, 1718
AccI GTMKAC 5 cut(s) 12, 1050, 3021, 3248, 3838
AccII CGCG 6 cut(s) 26, 101, 138, 164, 1143, 3028
AccIII TCCGGA 1 cut(s) 214
AclI AACGTT 1 cut(s) 3460
AclWI GGATC 7 cut(s) 206, 846, 1313, 2387, 2400, 4352, 4365
AcoI YGGCCR 4 cut(s) 357, 812, 3886, 4444
AcuI CTGAAG 7 cut(s) 1287, 1371, 2860, 3263, 3344, 4124, 4148
AcyI GRCGYC 2 cut(s) 96, 186
AflII CTTAAG 1 cut(s) 760
AflIII ACRYGT 2 cut(s) 868, 2984
AhlI ACTAGT 1 cut(s) 4502
AjiI CACGTC 1 cut(s) 49
AjnI CCWGG 9 cut(s) 173, 614, 1331, 2560, 2647, 2674, 2769, 3042, 3608
AjuI GAANNNNNNNTTGG 2 cut(s) 4146, 4178
AleI CACNNNNGTG 2 cut(s) 1002, 4044
Alw21I GWGCWC 4 cut(s) 113, 1125, 3388, 3931
Alw26I GTCTC 8 cut(s) 233, 578, 1389, 3551, 3651, 4080, 4162, 4193
AlwI GGATC 7 cut(s) 206, 846, 1313, 2387, 2400, 4352, 4365
AlwNI CAGNNNCTG 2 cut(s) 1739, 2776
Ama87I CYCGRG 1 cut(s) 106
Aor13HI TCCGGA 1 cut(s) 214
ArsI GACNNNNNNTTYG 2 cut(s) 2026, 2058
AseI ATTAAT 2 cut(s) 1464, 1626
Asp700I GAANNNNTTC 2 cut(s) 1973, 3335
AspLEI GCGC 6 cut(s) 26, 98, 103, 138, 1868, 3030
AspS9I GGNCC 8 cut(s) 171, 2323, 2564, 2669, 3538, 3606, 3947, 4238
AsuC2I CCSGG 2 cut(s) 62, 93
AsuNHI GCTAGC 1 cut(s) 2857
AvaI CYCGRG 1 cut(s) 106
AvaII GGWCC 5 cut(s) 171, 2323, 2564, 2669, 3538
AxyI CCTNAGG 1 cut(s) 2967
BaeGI GKGCMC 1 cut(s) 4084
BaeI ACNNNNGTAYC 4 cut(s) 2685, 2718, 3830, 3863
BalI TGGCCA 1 cut(s) 3888
BamHI GGATCC 2 cut(s) 2392, 4357
BanI GGYRCC 4 cut(s) 17, 95, 277, 4396
BanII GRGCYC 1 cut(s) 113
BarI GAAGNNNNNNTAC 2 cut(s) 218, 250
BauI CACGAG 1 cut(s) 4077
BbsI GAAGAC 3 cut(s) 278, 527, 1901
Bbv12I GWGCWC 4 cut(s) 113, 1125, 3388, 3931
BceAI ACGGC 8 cut(s) 31, 265, 372, 827, 2874, 3467, 4095, 4459
BciT130I CCWGG 9 cut(s) 175, 616, 1333, 2562, 2649, 2676, 2771, 3044, 3610
BciVI GTATCC 5 cut(s) 1428, 2041, 2299, 2993, 3858
BclI TGATCA 4 cut(s) 672, 1431, 3478, 4272
BcnI CCSGG 2 cut(s) 62, 93
BcoDI GTCTC 8 cut(s) 233, 578, 1389, 3551, 3651, 4080, 4162, 4193
BcuI ACTAGT 1 cut(s) 4502
BfaI CTAG 5 cut(s) 533, 785, 1887, 2858, 4503
BfmI CTRYAG 1 cut(s) 3249
BfoI RGCGCY 1 cut(s) 99
BfrI CTTAAG 1 cut(s) 760
BfuAI ACCTGC 1 cut(s) 2824
BfuI GTATCC 5 cut(s) 1428, 2041, 2299, 2993, 3858
BglI GCCNNNNNGGC 1 cut(s) 356
BglII AGATCT 3 cut(s) 2182, 2278, 2951
BmcAI AGTACT 1 cut(s) 317
Bme18I GGWCC 5 cut(s) 171, 2323, 2564, 2669, 3538
BmeT110I CYCGRG 1 cut(s) 106
BmgBI CACGTC 1 cut(s) 49
BmgT120I GGNCC 8 cut(s) 171, 2323, 2564, 2669, 3538, 3606, 3947, 4238
BmsI GCATC 8 cut(s) 337, 442, 868, 2308, 3474, 3670, 4357, 4504
BmtI GCTAGC 1 cut(s) 2861
BoxI GACNNNNGTC 4 cut(s) 8, 2562, 2877, 3543
BpiI GAAGAC 3 cut(s) 278, 527, 1901
BplI GAGNNNNNCTC 2 cut(s) 2616, 2648
BpmI CTGGAG 5 cut(s) 1494, 3326, 3410, 3658, 4454
Bpu10I CCTNAGC 2 cut(s) 1881, 3174
BpuEI CTTGAG 3 cut(s) 949, 976, 1021
BpuMI CCSGG 2 cut(s) 62, 93
Bsa29I ATCGAT 1 cut(s) 3091
BsaBI GATNNNNATC 1 cut(s) 4277
BsaHI GRCGYC 2 cut(s) 96, 186
BsaI GGTCTC 2 cut(s) 3651, 4162
BsaWI WCCGGW 2 cut(s) 214, 444
BsaXI ACNNNNNCTCC 2 cut(s) 1629, 1659
Bse118I RCCGGY 4 cut(s) 20, 76, 202, 3130
Bse21I CCTNAGG 1 cut(s) 2967
Bse3DI GCAATG 1 cut(s) 4133
Bse8I GATNNNNATC 1 cut(s) 4277
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 9 cut(s) 175, 616, 1333, 2562, 2649, 2676, 2771, 3044, 3610
BseCI ATCGAT 1 cut(s) 3091
BseJI GATNNNNATC 1 cut(s) 4277
BseMI GCAATG 1 cut(s) 4133
BseMII CTCAG 8 cut(s) 1529, 1910, 2321, 2610, 2630, 2649, 2753, 2958
BseSI GKGCMC 1 cut(s) 4084
BseX3I CGGCCG 1 cut(s) 4444
BseYI CCCAGC 2 cut(s) 3030, 3953
BsgI GTGCAG 4 cut(s) 726, 2037, 2115, 2802
Bsh1236I CGCG 6 cut(s) 26, 101, 138, 164, 1143, 3028
Bsh1285I CGRYCG 2 cut(s) 81, 4447
BshNI GGYRCC 4 cut(s) 17, 95, 277, 4396
BshVI ATCGAT 1 cut(s) 3091
BsiEI CGRYCG 2 cut(s) 81, 4447
BsiHKAI GWGCWC 4 cut(s) 113, 1125, 3388, 3931
BsiHKCI CYCGRG 1 cut(s) 106
BsiSI CCGG 8 cut(s) 21, 62, 77, 93, 203, 215, 445, 3131
BsiWI CGTACG 2 cut(s) 27, 3933
BslFI GGGAC 2 cut(s) 2125, 2480
BsmAI GTCTC 8 cut(s) 233, 578, 1389, 3551, 3651, 4080, 4162, 4193
BsmBI CGTCTC 1 cut(s) 233
BsmFI GGGAC 2 cut(s) 2125, 2480
BsmI GAATGC 2 cut(s) 1807, 3573
Bso31I GGTCTC 2 cut(s) 3651, 4162
BsoBI CYCGRG 1 cut(s) 106
Bsp1286I GDGCHC 5 cut(s) 113, 1125, 3388, 3931, 4084
Bsp13I TCCGGA 1 cut(s) 214
BspCNI CTCAG 8 cut(s) 1530, 1909, 2322, 2609, 2629, 2648, 2752, 2959
BspDI ATCGAT 1 cut(s) 3091
BspEI TCCGGA 1 cut(s) 214
BspFNI CGCG 6 cut(s) 26, 101, 138, 164, 1143, 3028
BspHI TCATGA 2 cut(s) 294, 540
BspMI ACCTGC 1 cut(s) 2824
BspOI GCTAGC 1 cut(s) 2861
BspPI GGATC 7 cut(s) 206, 846, 1313, 2387, 2400, 4352, 4365
BspQI GCTCTTC 2 cut(s) 1413, 1497
BspT107I GGYRCC 4 cut(s) 17, 95, 277, 4396
BspTI CTTAAG 1 cut(s) 760
BspTNI GGTCTC 2 cut(s) 3651, 4162
BsrDI GCAATG 1 cut(s) 4133
BsrFI RCCGGY 4 cut(s) 20, 76, 202, 3130
BssAI RCCGGY 4 cut(s) 20, 76, 202, 3130
BssNAI GTATAC 1 cut(s) 3022
BssNI GRCGYC 2 cut(s) 96, 186
BssSI CACGAG 1 cut(s) 4077
BssT1I CCWWGG 8 cut(s) 189, 469, 1322, 2142, 2396, 2585, 3598, 3776
Bst1107I GTATAC 1 cut(s) 3022
Bst2BI CACGAG 1 cut(s) 4077
Bst2UI CCWGG 9 cut(s) 175, 616, 1333, 2562, 2649, 2676, 2771, 3044, 3610
Bst6I CTCTTC 9 cut(s) 221, 771, 1413, 1497, 1559, 1866, 3213, 3320, 4554
BstACI GRCGYC 2 cut(s) 96, 186
BstAFI CTTAAG 1 cut(s) 760
BstAPI GCANNNNNTGC 1 cut(s) 2093
BstDSI CCRYGG 1 cut(s) 1651
BstEII GGTNACC 2 cut(s) 3961, 4037
BstENI CCTNNNNNAGG 2 cut(s) 2013, 2559
BstFNI CGCG 6 cut(s) 26, 101, 138, 164, 1143, 3028
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 6 cut(s) 26, 98, 103, 138, 1868, 3030
BstMAI GTCTC 8 cut(s) 233, 578, 1389, 3551, 3651, 4080, 4162, 4193
BstMCI CGRYCG 2 cut(s) 81, 4447
BstNI CCWGG 9 cut(s) 175, 616, 1333, 2562, 2649, 2676, 2771, 3044, 3610
BstNSI RCATGY 5 cut(s) 374, 2988, 3309, 3835, 4418
BstPAI GACNNNNGTC 4 cut(s) 8, 2562, 2877, 3543
BstPI GGTNACC 2 cut(s) 3961, 4037
BstSFI CTRYAG 1 cut(s) 3249
BstSLI GKGCMC 1 cut(s) 4084
BstUI CGCG 6 cut(s) 26, 101, 138, 164, 1143, 3028
BstV2I GAAGAC 3 cut(s) 278, 527, 1901
BstX2I RGATCY 5 cut(s) 2182, 2278, 2392, 2951, 4357
BstXI CCANNNNNNTGG 1 cut(s) 3777
BstYI RGATCY 5 cut(s) 2182, 2278, 2392, 2951, 4357
BstZ17I GTATAC 1 cut(s) 3022
BstZI CGGCCG 1 cut(s) 4444
Bsu15I ATCGAT 1 cut(s) 3091
Bsu36I CCTNAGG 1 cut(s) 2967
BsuI GTATCC 5 cut(s) 1428, 2041, 2299, 2993, 3858
BsuTUI ATCGAT 1 cut(s) 3091
BtgI CCRYGG 1 cut(s) 1651
BtrI CACGTC 1 cut(s) 49
BtsI GCAGTG 2 cut(s) 2079, 4264
BtsIMutI CAGTG 7 cut(s) 586, 1412, 1702, 1977, 2079, 3823, 4264
BveI ACCTGC 1 cut(s) 2824
CaiI CAGNNNCTG 2 cut(s) 1739, 2776
CciI TCATGA 2 cut(s) 294, 540
CfoI GCGC 6 cut(s) 26, 98, 103, 138, 1868, 3030
Cfr10I RCCGGY 4 cut(s) 20, 76, 202, 3130
Cfr13I GGNCC 8 cut(s) 171, 2323, 2564, 2669, 3538, 3606, 3947, 4238
ClaI ATCGAT 1 cut(s) 3091
CseI GACGC 4 cut(s) 29, 194, 1528, 3824
CsiI ACCWGGT 1 cut(s) 2674
CspCI CAANNNNNGTGG 4 cut(s) 2602, 2637, 2646, 2681
DinI GGCGCC 1 cut(s) 97
DraI TTTAAA 2 cut(s) 660, 1171
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 4 cut(s) 357, 812, 3886, 4444
EagI CGGCCG 1 cut(s) 4444
Eam1104I CTCTTC 9 cut(s) 221, 771, 1413, 1497, 1559, 1866, 3213, 3320, 4554
EarI CTCTTC 9 cut(s) 221, 771, 1413, 1497, 1559, 1866, 3213, 3320, 4554
Ecl136II GAGCTC 1 cut(s) 111
EclXI CGGCCG 1 cut(s) 4444
Eco130I CCWWGG 8 cut(s) 189, 469, 1322, 2142, 2396, 2585, 3598, 3776
Eco147I AGGCCT 1 cut(s) 2401
Eco24I GRGCYC 1 cut(s) 113
Eco31I GGTCTC 2 cut(s) 3651, 4162
Eco32I GATATC 2 cut(s) 3864, 4375
Eco47I GGWCC 5 cut(s) 171, 2323, 2564, 2669, 3538
Eco52I CGGCCG 1 cut(s) 4444
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 7 cut(s) 1287, 1371, 2860, 3263, 3344, 4124, 4148
Eco81I CCTNAGG 1 cut(s) 2967
Eco88I CYCGRG 1 cut(s) 106
Eco91I GGTNACC 2 cut(s) 3961, 4037
EcoICRI GAGCTC 1 cut(s) 111
EcoNI CCTNNNNNAGG 2 cut(s) 2013, 2559
EcoO109I RGGNCCY 2 cut(s) 3947, 4238
EcoO65I GGTNACC 2 cut(s) 3961, 4037
EcoRI GAATTC 1 cut(s) 3107
EcoRII CCWGG 9 cut(s) 173, 614, 1331, 2560, 2647, 2674, 2769, 3042, 3608
EcoRV GATATC 2 cut(s) 3864, 4375
EcoT14I CCWWGG 8 cut(s) 189, 469, 1322, 2142, 2396, 2585, 3598, 3776
EcoT38I GRGCYC 1 cut(s) 113
EgeI GGCGCC 1 cut(s) 97
EheI GGCGCC 1 cut(s) 97
ErhI CCWWGG 8 cut(s) 189, 469, 1322, 2142, 2396, 2585, 3598, 3776
Esp3I CGTCTC 1 cut(s) 233
FalI AAGNNNNNCTT 6 cut(s) 2389, 2421, 3095, 3127, 3167, 3199
FaqI GGGAC 2 cut(s) 2125, 2480
FauNDI CATATG 2 cut(s) 1573, 3534
FbaI TGATCA 4 cut(s) 672, 1431, 3478, 4272
FblI GTMKAC 5 cut(s) 12, 1050, 3021, 3248, 3838
FriOI GRGCYC 1 cut(s) 113
FspBI CTAG 5 cut(s) 533, 785, 1887, 2858, 4503
GlaI GCGC 6 cut(s) 25, 97, 102, 137, 1867, 3029
GsaI CCCAGC 2 cut(s) 3034, 3957
GsuI CTGGAG 5 cut(s) 1494, 3326, 3410, 3658, 4454
HaeII RGCGCY 1 cut(s) 99
HapII CCGG 8 cut(s) 21, 62, 77, 93, 203, 215, 445, 3131
HgaI GACGC 4 cut(s) 29, 194, 1528, 3824
HhaI GCGC 6 cut(s) 26, 98, 103, 138, 1868, 3030
Hin1I GRCGYC 2 cut(s) 96, 186
Hin6I GCGC 6 cut(s) 24, 96, 101, 136, 1866, 3028
HinP1I GCGC 6 cut(s) 24, 96, 101, 136, 1866, 3028
HincII GTYRAC 3 cut(s) 829, 1051, 3973
HindII GTYRAC 3 cut(s) 829, 1051, 3973
HindIII AAGCTT 2 cut(s) 1901, 3893
HpaII CCGG 8 cut(s) 21, 62, 77, 93, 203, 215, 445, 3131
Hpy166II GTNNAC 9 cut(s) 13, 829, 1051, 2607, 2672, 3022, 3249, 3839, 3973
Hpy8I GTNNAC 9 cut(s) 13, 829, 1051, 2607, 2672, 3022, 3249, 3839, 3973
Hpy99I CGWCG 2 cut(s) 53, 188
HpyCH4IV ACGT 4 cut(s) 48, 362, 870, 3460
HpySE526I ACGT 4 cut(s) 48, 362, 870, 3460
Hsp92I GRCGYC 2 cut(s) 96, 186
HspAI GCGC 6 cut(s) 24, 96, 101, 136, 1866, 3028
KasI GGCGCC 1 cut(s) 95
Kpn2I TCCGGA 1 cut(s) 214
KroI GCCGGC 1 cut(s) 202
KroNI GCCGGC 1 cut(s) 204
Ksp22I TGATCA 4 cut(s) 672, 1431, 3478, 4272
LguI GCTCTTC 2 cut(s) 1413, 1497
LweI GCATC 8 cut(s) 337, 442, 868, 2308, 3474, 3670, 4357, 4504
MabI ACCWGGT 1 cut(s) 2674
MaeI CTAG 5 cut(s) 533, 785, 1887, 2858, 4503
MaeII ACGT 4 cut(s) 48, 362, 870, 3460
MaeIII GTNAC 7 cut(s) 41, 1741, 3053, 3237, 3961, 4037, 4486
MfeI CAATTG 3 cut(s) 1103, 1218, 2960
MflI RGATCY 5 cut(s) 2182, 2278, 2392, 2951, 4357
MhlI GDGCHC 5 cut(s) 113, 1125, 3388, 3931, 4084
MlsI TGGCCA 1 cut(s) 3888
MluNI TGGCCA 1 cut(s) 3888
Mly113I GGCGCC 1 cut(s) 96
MlyI GAGTC 8 cut(s) 300, 731, 2377, 2501, 3113, 3155, 3553, 3770
MmeI TCCRAC 5 cut(s) 105, 2602, 2898, 3141, 4177
Mox20I TGGCCA 1 cut(s) 3888
MroI TCCGGA 1 cut(s) 214
MroNI GCCGGC 1 cut(s) 202
MroXI GAANNNNTTC 2 cut(s) 1973, 3335
MscI TGGCCA 1 cut(s) 3888
MslI CAYNNNNRTG 7 cut(s) 873, 1002, 1614, 2906, 4044, 4314, 4479
Msp20I TGGCCA 1 cut(s) 3888
MspA1I CMGCKG 5 cut(s) 1159, 1736, 1748, 2699, 3254
MspCI CTTAAG 1 cut(s) 760
MspI CCGG 8 cut(s) 21, 62, 77, 93, 203, 215, 445, 3131
MteI GCGCNGCGC 1 cut(s) 99
MunI CAATTG 3 cut(s) 1103, 1218, 2960
Mva1269I GAATGC 2 cut(s) 1807, 3573
MvaI CCWGG 9 cut(s) 175, 616, 1333, 2562, 2649, 2676, 2771, 3044, 3610
MvnI CGCG 6 cut(s) 26, 101, 138, 164, 1143, 3028
NaeI GCCGGC 1 cut(s) 204
NarI GGCGCC 1 cut(s) 96
NciI CCSGG 2 cut(s) 62, 93
NdeI CATATG 2 cut(s) 1573, 3534
NgoMIV GCCGGC 1 cut(s) 202
NheI GCTAGC 1 cut(s) 2857
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 5 cut(s) 41, 1741, 3961, 4037, 4486
NspI RCATGY 5 cut(s) 374, 2988, 3309, 3835, 4418
OliI CACNNNNGTG 2 cut(s) 1002, 4044
PagI TCATGA 2 cut(s) 294, 540
PceI AGGCCT 1 cut(s) 2401
PciI ACATGT 1 cut(s) 2984
PciSI GCTCTTC 2 cut(s) 1413, 1497
PcsI WCGNNNNNNNCGW 2 cut(s) 2887, 3457
PctI GAATGC 2 cut(s) 1807, 3573
PdiI GCCGGC 1 cut(s) 204
PdmI GAANNNNTTC 2 cut(s) 1973, 3335
Pfl23II CGTACG 2 cut(s) 27, 3933
PflMI CCANNNNNTGG 4 cut(s) 319, 562, 821, 1718
PleI GAGTC 8 cut(s) 299, 730, 2376, 2501, 3113, 3154, 3552, 3769
PluTI GGCGCC 1 cut(s) 99
PpsI GAGTC 8 cut(s) 299, 730, 2376, 2501, 3113, 3154, 3552, 3769
PscI ACATGT 1 cut(s) 2984
PshAI GACNNNNGTC 4 cut(s) 8, 2562, 2877, 3543
PshBI ATTAAT 2 cut(s) 1464, 1626
PsiI TTATAA 2 cut(s) 1776, 3882
Psp124BI GAGCTC 1 cut(s) 113
Psp1406I AACGTT 1 cut(s) 3460
Psp6I CCWGG 9 cut(s) 173, 614, 1331, 2560, 2647, 2674, 2769, 3042, 3608
PspEI GGTNACC 2 cut(s) 3961, 4037
PspFI CCCAGC 2 cut(s) 3030, 3953
PspGI CCWGG 9 cut(s) 173, 614, 1331, 2560, 2647, 2674, 2769, 3042, 3608
PspLI CGTACG 2 cut(s) 27, 3933
PspPI GGNCC 8 cut(s) 171, 2323, 2564, 2669, 3538, 3606, 3947, 4238
PstNI CAGNNNCTG 2 cut(s) 1739, 2776
PsuI RGATCY 5 cut(s) 2182, 2278, 2392, 2951, 4357
PvuII CAGCTG 5 cut(s) 1159, 1736, 1748, 2699, 3254
RseI CAYNNNNRTG 7 cut(s) 873, 1002, 1614, 2906, 4044, 4314, 4479
SacI GAGCTC 1 cut(s) 113
SalI GTCGAC 1 cut(s) 1049
SapI GCTCTTC 2 cut(s) 1413, 1497
Sau96I GGNCC 8 cut(s) 171, 2323, 2564, 2669, 3538, 3606, 3947, 4238
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 8 cut(s) 300, 731, 2377, 2501, 3113, 3155, 3553, 3770
SduI GDGCHC 5 cut(s) 113, 1125, 3388, 3931, 4084
SexAI ACCWGGT 1 cut(s) 2674
SfaNI GCATC 8 cut(s) 337, 442, 868, 2308, 3474, 3670, 4357, 4504
SfcI CTRYAG 1 cut(s) 3249
SfoI GGCGCC 1 cut(s) 97
SgrAI CRCCGGYG 1 cut(s) 20
SinI GGWCC 5 cut(s) 171, 2323, 2564, 2669, 3538
SmiMI CAYNNNNRTG 7 cut(s) 873, 1002, 1614, 2906, 4044, 4314, 4479
SmlI CTYRAG 4 cut(s) 760, 928, 955, 1000
SmoI CTYRAG 4 cut(s) 760, 928, 955, 1000
SpeI ACTAGT 1 cut(s) 4502
SseBI AGGCCT 1 cut(s) 2401
SspDI GGCGCC 1 cut(s) 95
SspI AATATT 4 cut(s) 1717, 2122, 2198, 3870
SspMI CTAG 5 cut(s) 533, 785, 1887, 2858, 4503
SstI GAGCTC 1 cut(s) 113
StuI AGGCCT 1 cut(s) 2401
StyI CCWWGG 8 cut(s) 189, 469, 1322, 2142, 2396, 2585, 3598, 3776
TaiI ACGT 4 cut(s) 51, 365, 873, 3463
TatI WGTACW 4 cut(s) 315, 751, 891, 1255
TauI GCSGC 5 cut(s) 101, 161, 164, 2258, 4110
TscAI CASTG 7 cut(s) 586, 1419, 1702, 1984, 2086, 3823, 4264
TseFI GTSAC 5 cut(s) 41, 1741, 3961, 4037, 4486
Tsp45I GTSAC 5 cut(s) 41, 1741, 3961, 4037, 4486
TspGWI ACGGA 6 cut(s) 257, 846, 1547, 1640, 3893, 3921
TspRI CASTG 7 cut(s) 586, 1419, 1702, 1984, 2086, 3823, 4264
Van91I CCANNNNNTGG 4 cut(s) 319, 562, 821, 1718
Vha464I CTTAAG 1 cut(s) 760
VpaK11BI GGWCC 5 cut(s) 171, 2323, 2564, 2669, 3538
VspI ATTAAT 2 cut(s) 1464, 1626
XagI CCTNNNNNAGG 2 cut(s) 2013, 2559
XceI RCATGY 5 cut(s) 374, 2988, 3309, 3835, 4418
XmiI GTMKAC 5 cut(s) 12, 1050, 3021, 3248, 3838
XmnI GAANNNNTTC 2 cut(s) 1973, 3335
XspI CTAG 5 cut(s) 533, 785, 1887, 2858, 4503
ZrmI AGTACT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.