Rroxscaffold_4G00328150

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008765
Physical Location & Seq
Forward (+)
61052401 .. 61062767
10367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328150.1

Sequence Viewer

Length: 4644 bp
ATGACGAAGGTGTACGGAACAGGCGCGTACGATTTCAAGCGCCACCATGTTGCCGAGTACCCGGTGGAGCTGAACCAACAGCTCGGAGACAAGCCGGTGGAGGCTAAACCCGGCGCGGCGCTGCCGAGCTCGATAACGCTGTCGGAGATTCAGAGGGACCAGCTCACTATGATTGCCGCTGCGAATTGGTCCAAAGCCGGCGGCGACGCCGCGCTGGTGAAGAAGGCGTTCGACGCGGAGCTGGTGAAGAAGATTTACGAGACGGAGTTGTGTGTGAAGGAGGGGCAGAGGAAGACGGTGCCATTGCAGAGAGTGATGATTTTGGAGGTCAGCCAGTACTTGGAGAACTATCTCTTTCCCAATTTCGATCCCGAAACGGCGACGTTCGAGCACGTTATGTCCATGATTCTCATGGTCAATGAGAAGTTTCGAGAGAATGTGGCAGCTTGGGTATGCTTTCATGACCGGAAAGATGCGTTTAAAGGATTCCTTGGAAGGGTTCTTCGACTTAAATCGGAAAGAGAATTAAGTATAGCAGAGAAGACAAATTATCTAGTATTCATGATAAATGCATTTCAGAGTTTGGAAGATGAGATAGTCAGTGAGACTGTCCTTAGGTTGGCAAGTTTCCAATCCTGGCACAGTTTGTCGTATGGTCGTTTTCAGATGGAGCTAGGTTTAAATTCAGATTTGATCAAGAAATGGAGAAGGATGATAAAGAGGGAAGCTGCAAAACATGGAGAGTCTTTTAACCCCTCAACTACACTTGAAGTCCAGTTTTTGAGAAACCTCATCGAAGAATTCCTGGAGATACTTGATTCCAAAGTCTTACCGCCAAATCATGGTGTCAATGGAGAGGATCAACTTCTTGCTGCCAATGGAATGGAACACATTGATGATGCTTGTGTTTTGTACTGTGAGAGGTTTATAGAATTTCTTATTGACCTCTTGAGTCAACTACCAACAAGGAGGTACTTGAGGCCACTTGTTGCTGATGTTGCTGTTGTTCCGAAATGCCACTTGAGTGCTTTGTATAGACATGAAAAGGGGAAGCTCTTTACCCAACTTGTTGACTTGCTGCAGTTTTATGAAGGATTTGAGATTAATGATAATGTCGGGAAACAATTGACGGATGATGAAGTGCTCCAATCTCATTATGATCGAGTACAATCTTTCCAGCTGCTTGCTTTTAAAAAGATTCCTAAGTTGCAAGAGCTTGCATTGGCCAACATTGGTTCAATTGACAACCGAAATGATCTCACTAAGAGATTGTCTGTTCTTTCTCATGAAGAATTGAAGGATTTGGTTTGTTCAAAGTTGAAACTGATTTCTAAGAATGATCCTTGGTCATCCAGGGTTGATTTCCTTACTGAAGTGATGGTTTCCTTTTTCAAGAGGCAACAGTCTCAAAAAGAAAAAATAAATGCTCTTCCACTGTATCCAAATGAGCTTATCATGTGGGATGAAAGCCTTGTGCCAAGCATTAATTACTCTGGAGAAGGTTGTCTGGCTCTTCCAAAACTTAATTTACAATTTTTGACACTGCATGATTATCTCCTGAGAAATTTTGATCTCTTCCGCCTTGAATCAACATATGAGATCCGTGAGGATATTCAGGAGGCTGTGCCACATCTGAATGCCTGCATTAATAATGAGGGAGAAACTGCTTTCCGTGGTTGGTCAAGGATGGCTGTGCCGATTAAAGAATTCAAGATCAGTGAGGTAAAACAGCCAAATATTGGAGAAGTTAAGCCAGCTGCTGTGACAGCAGAAATCACTTTTAGTATATCCAGTTATAGAGCACAGGTACGATCAGAATGGAATGCTCTTAAAGAGCATGATGTTCTATTTTTACTTTCCATACGCCCTTCCTTTGAGCCTCTAAGTGCAGAAGAGGGTGCCAAGGCTAGTGTGCCTCAGAGGCTTGGTCTTCAATATGTACGGGGTTGTGAAATCATTGAGGTTCGTGATGAGGAGGGAACTCTAATGAATGATTTCACTGGAAGAATTAAACGGGATGAGTGGAAGCCTCCAAAAGGAGAGTTGAGAACTTTGACTGTTGCTTTGGATACCGCTCAGTACTACATGGATGTTAGCAACACTGCTGCAAAGGGTGCAGAAGATGTTTATGGGACATTCAATATTTTGATGAGGAGAAAGCCCAAGGAAAATAATTTCAAAGCAATTTTAGAATCCATTAGAGATCTTATGAATGAATATTGTATTGTTCCTGACTGGTTGCACAAAACATTTCTGGGGTATGGTAATCCTTCTGCCGCACAGTGGACTAATATGCCAGATCTCTTAGAAACCGTAGATTTCAAAGATACTTTCCTTGATGCAGACCATCTGAAGGAATGTTTTCCAGATGATCAGGTTTTCTTTGTTAGTCCAGATGGTATAGAAAATATGGATCCAAGGCCTCCTTTTCGAATAAAGCTCCCCAAGAAGATCAAAAGCAGCACTAATGCTCTTCCGGGCAATAAGAAAGCAAAGATTAATTCTATGAGTGATGCCCCCATTGAGGACTCTGATAGTGAGAAAGAAAAATTTGTTGTTGAGGCATACACCCCTCCTGACCCAGGTCCTTATCCGCAAGACCAGCCAAGGCAGAACTCAGTTAGATTTACACCCACTCAGGTTGGAGCAATCCTATCAGGTATTCAGCCAGGACTAACAATGGTTGTCGGTCCACCTGGTACGGGAAAGACTGATACAGCCGTGCAAATTTTGAATGTTCTGTATCATAATTGTCCATCTCAGAGAACATTGATAATTACTCACTCAAACCAGGCTCTAAATGACCTTTTTGAAAAGATAATGCAGAGGGATGTACCTGCACGCTATCTTCTTCGACTTGGGCAAGGAGAACAAGAGCTAGCAACTGATCTTGACTTCAGTCGGCAAGGCCGTGTCAATGCCATGCTTGTGAGGCGGTTGGAGTTGCTTAGTGAAGTGGAAAGGCTGGCAAGATCTCTCCAATTGCCCGAGGATGTTGGTTATACATGTGAAACTGCTGGATACTTTTGGTTGCTTCATGTATACTCGCGCTGGGAGCAATTCCTGGCTGCTTGTAACCAAAATAAAAATAAACAATCATTTGTTAAAGATCGATTTCCCTTCAAGGAATTCTTCTCCAACGCACCAAAGCCAGTTTTTACTGGTGAGTCATTTGAGAAAGATATGCGGGCAGCTAAGGGTTGCTTTCGTCATCTAAAGACTGTGTTTCAAGAGCTTGAAGAGTGTAGGGCTTTTGAGTTACTGAAGTCTACAGCTGATCGAGCAAACTACTTGATGACGAAGCAGGCAAAGATAGTTGCCATGACATGCACTCACGCAGCTCTGAAGAGGAAGGATTTTCTCCAGTTAGGTTTCAAGTATGATAACTTGCTGATGGAAGAAAGTGCTCAAATTTTGGAGATCGAAACTTTCATTCCAATGTTGCTCCAGAGGCAGGAAGATGGTTATGCACGGCTTAAACGTTGTATCCTGATTGGTGATCATCACCAGTTGCCTCCTGTTGTCAAGAATATGGCCTTTCAAAAGTATAGCCACATGGACCAGAGTCTCTTTACAAGGTTCGTCCGTTTGGGCATTCCTTATATTGAACTTAATGCCCAGGGAAGGGCCAGGCCAAGTATAGCCAAACTTTACAACTGGAGGTATAGAGAACTAGGAGACCTTCCTTATGTAAAGGAAGATGCTGTTTACAATAGAGCAAATTCTGGATTCTCCTTTGAATATCAATTGGTAGATGTACCAGATTACCATGATAGAGGTGAGTCTGCTCCATCACCTTGGTTCTACCAAAATGAGGGAGAAGCTGAATATATCGTCAGTGTGTATATCTACATGCGTCTACTTGGGTACCCTGCAAACAAGATATCAATATTGACCACTTACAATGGCCAGAAGCTTTTAATCCGTGATGTTATCAACAGACGATGTGCTCCGTATGACTTCATAGGCCCTCCCAGTAAGGTTACAACAGTTGACAAATTTCAGGGTCAGCAAAATGATTTTATATTGCTCTCTCTTGTGCGTACTCGCTTTGTGGGTCACCTTCGTGATGTTAGGAGATTGATTGTTGCCATGTCTCGTGCCCGATTGGGTCTGTATGTGTTTTGCCGCCGTTCTTTGTTTGAGCAATGCTACGAACTTCAGCCTACCTTCCAACTTTTACTTCAGAGACCTGATCGCCTTGCTCTGAATTTTAATGAGACTACACCACACACAGAACGCCATGTTGAAGAAACGGGGCCTGTCCATCTTGTAAGCAGTGTTGATGAGATGATCAGTATCTACCAGCAGCTTTATGCGGTGAAGTTTCATCAGTATGTTGCTCCTTCAAGTCAATTAGTTCCATCCATGGATGCATTGGGAGAGCAAACAAGTCAGCAAACTGATATGTCTGGGCACATTCCTATGGATGCAGATATCCCTGTATCTGCTGATGCGGCGCCAGACGATACACTGCATGGAAACAATTCAGAACTGGGCGAAGAATTGGATGTCGCAAATGGCCAAAAAGTGGATTCGTCGATTGAGAATCATACAAAGGGGGTGACGGAAATGGAAACTAGTCTGGATGATGCACATGTGGCTCCCGAAAGCAATTCGGATCAAGTGAAAATGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

1547

Amino Acids

177.0

Weight (kDa)

5.71

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 55 - 468 4.9e-134 Intron-binding protein aquarius N-terminal
Aquarius_N_2nd PF21143 550 - 719 7.4e-57 Intron-binding protein aquarius, beta-barrel
Aquarius_N_3rd PF21144 769 - 868 6.5e-21 Intron-binding protein aquarius insert domain
AAA_11 PF13086 881 - 1177 1.2e-28 AAA domain
AAA_12 PF13087 1186 - 1378 6.6e-27 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2845
Acc65I GGTACC 1 cut(s) 3867
AccB1I GGYRCC 4 cut(s) 298, 1898, 3867, 4459
AccB7I CCANNNNNTGG 4 cut(s) 340, 842, 1739, 4531
AccBSI CCGCTC 1 cut(s) 2075
AccI GTMKAC 3 cut(s) 3042, 3269, 3859
AccII CGCG 5 cut(s) 26, 116, 212, 236, 3049
AclI AACGTT 1 cut(s) 3481
AclWI GGATC 7 cut(s) 362, 867, 1334, 1594, 2408, 2421, 4629
AcoI YGGCCR 3 cut(s) 1224, 3907, 4522
AcuI CTGAAG 7 cut(s) 1392, 2372, 2881, 3284, 3365, 4145, 4169
AcyI GRCGYC 2 cut(s) 207, 4460
AflIII ACRYGT 2 cut(s) 3005, 4597
AhlI ACTAGT 1 cut(s) 4580
AjuI GAANNNNNNNTTGG 2 cut(s) 4167, 4199
AleI CACNNNNGTG 2 cut(s) 1023, 4065
Alw21I GWGCWC 6 cut(s) 131, 393, 1146, 1804, 3409, 3952
Alw26I GTCTC 9 cut(s) 81, 254, 599, 1410, 3572, 3672, 4101, 4183, 4214
AlwI GGATC 7 cut(s) 362, 867, 1334, 1594, 2408, 2421, 4629
AlwNI CAGNNNCTG 1 cut(s) 1760
Ama87I CYCGRG 1 cut(s) 2987
ArsI GACNNNNNNTTYG 2 cut(s) 2047, 2079
AseI ATTAAT 4 cut(s) 1104, 1485, 1647, 2499
Asp700I GAANNNNTTC 4 cut(s) 227, 1994, 2361, 4486
Asp718I GGTACC 1 cut(s) 3867
AspLEI GCGC 7 cut(s) 26, 42, 116, 121, 214, 3051, 4462
AspS9I GGNCC 8 cut(s) 157, 189, 2585, 2690, 3559, 3627, 3968, 4259
AsuC2I CCSGG 3 cut(s) 62, 111, 2478
AsuII TTCGAA 1 cut(s) 2431
AsuNHI GCTAGC 1 cut(s) 2878
AvaI CYCGRG 1 cut(s) 2987
AvaII GGWCC 5 cut(s) 157, 189, 2585, 2690, 3559
AxyI CCTNAGG 1 cut(s) 614
BaeGI GKGCMC 2 cut(s) 4105, 4419
BaeI ACNNNNGTAYC 4 cut(s) 2706, 2739, 3851, 3884
BalI TGGCCA 3 cut(s) 1226, 3909, 4524
BamHI GGATCC 1 cut(s) 2413
BanI GGYRCC 4 cut(s) 298, 1898, 3867, 4459
BanII GRGCYC 1 cut(s) 131
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
BauI CACGAG 1 cut(s) 4098
BbsI GAAGAC 3 cut(s) 299, 548, 1922
Bbv12I GWGCWC 6 cut(s) 131, 393, 1146, 1804, 3409, 3952
BceAI ACGGC 5 cut(s) 393, 2705, 2895, 3488, 4116
BciVI GTATCC 4 cut(s) 1449, 2062, 3014, 3497
BclI TGATCA 4 cut(s) 693, 2371, 3499, 4293
BcnI CCSGG 3 cut(s) 62, 111, 2478
BcoDI GTCTC 9 cut(s) 81, 254, 599, 1410, 3572, 3672, 4101, 4183, 4214
BcuI ACTAGT 1 cut(s) 4580
BfaI CTAG 6 cut(s) 554, 674, 1908, 2879, 3674, 4581
BfmI CTRYAG 2 cut(s) 1079, 3270
BfoI RGCGCY 3 cut(s) 43, 122, 4463
BfuAI ACCTGC 1 cut(s) 2845
BfuI GTATCC 4 cut(s) 1449, 2062, 3014, 3497
BglI GCCNNNNNGGC 1 cut(s) 1921
BglII AGATCT 3 cut(s) 2203, 2299, 2972
BmcAI AGTACT 2 cut(s) 338, 2081
Bme18I GGWCC 5 cut(s) 157, 189, 2585, 2690, 3559
BmeT110I CYCGRG 1 cut(s) 2987
BmgT120I GGNCC 8 cut(s) 157, 189, 2585, 2690, 3559, 3627, 3968, 4259
BmiI GGNNCC 8 cut(s) 158, 300, 1900, 2415, 3869, 4260, 4461, 4605
BmrI ACTGGG 2 cut(s) 3969, 4505
BmsI GCATC 9 cut(s) 463, 889, 2329, 2503, 3691, 4363, 4420, 4444, 4582
BmtI GCTAGC 1 cut(s) 2882
BmuI ACTGGG 2 cut(s) 3969, 4505
BoxI GACNNNNGTC 3 cut(s) 2583, 2898, 3564
BpiI GAAGAC 3 cut(s) 299, 548, 1922
BpmI CTGGAG 5 cut(s) 827, 1515, 3347, 3431, 3679
Bpu10I CCTNAGC 1 cut(s) 3195
Bpu14I TTCGAA 1 cut(s) 2431
BpuEI CTTGAG 3 cut(s) 970, 997, 1042
BpuMI CCSGG 3 cut(s) 62, 111, 2478
Bsa29I ATCGAT 1 cut(s) 3112
BsaBI GATNNNNATC 1 cut(s) 4298
BsaHI GRCGYC 2 cut(s) 207, 4460
BsaI GGTCTC 2 cut(s) 3672, 4183
BsaWI WCCGGW 1 cut(s) 465
Bse118I RCCGGY 2 cut(s) 94, 197
Bse21I CCTNAGG 1 cut(s) 614
Bse3DI GCAATG 2 cut(s) 302, 4154
Bse8I GATNNNNATC 1 cut(s) 4298
BseCI ATCGAT 1 cut(s) 3112
BseJI GATNNNNATC 1 cut(s) 4298
BseMI GCAATG 2 cut(s) 302, 4154
BseMII CTCAG 6 cut(s) 1550, 1931, 2090, 2631, 2651, 2774
BseRI GAGGAG 2 cut(s) 1988, 2167
BseSI GKGCMC 2 cut(s) 4105, 4419
BseYI CCCAGC 1 cut(s) 3051
BsgI GTGCAG 3 cut(s) 1908, 2136, 2823
Bsh1236I CGCG 5 cut(s) 26, 116, 212, 236, 3049
BshNI GGYRCC 4 cut(s) 298, 1898, 3867, 4459
BshVI ATCGAT 1 cut(s) 3112
BsiHKAI GWGCWC 6 cut(s) 131, 393, 1146, 1804, 3409, 3952
BsiHKCI CYCGRG 1 cut(s) 2987
BsiSI CCGG 6 cut(s) 62, 95, 111, 198, 466, 2477
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 2 cut(s) 170, 2146
BsmAI GTCTC 9 cut(s) 81, 254, 599, 1410, 3572, 3672, 4101, 4183, 4214
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 2 cut(s) 170, 2146
BsmI GAATGC 3 cut(s) 1642, 1828, 3594
Bso31I GGTCTC 2 cut(s) 3672, 4183
BsoBI CYCGRG 1 cut(s) 2987
Bsp119I TTCGAA 1 cut(s) 2431
Bsp1286I GDGCHC 8 cut(s) 131, 393, 1146, 1804, 3409, 3952, 4105, 4419
Bsp19I CCATGG 1 cut(s) 4368
BspCNI CTCAG 6 cut(s) 1551, 1930, 2089, 2630, 2650, 2773
BspDI ATCGAT 1 cut(s) 3112
BspFNI CGCG 5 cut(s) 26, 116, 212, 236, 3049
BspHI TCATGA 3 cut(s) 460, 561, 1285
BspLI GGNNCC 8 cut(s) 158, 300, 1900, 2415, 3869, 4260, 4461, 4605
BspMAI CTGCAG 1 cut(s) 1083
BspMI ACCTGC 1 cut(s) 2845
BspOI GCTAGC 1 cut(s) 2882
BspPI GGATC 7 cut(s) 362, 867, 1334, 1594, 2408, 2421, 4629
BspQI GCTCTTC 3 cut(s) 1434, 1518, 2478
BspT104I TTCGAA 1 cut(s) 2431
BspT107I GGYRCC 4 cut(s) 298, 1898, 3867, 4459
BspTNI GGTCTC 2 cut(s) 3672, 4183
BsrBI CCGCTC 1 cut(s) 2075
BsrDI GCAATG 2 cut(s) 302, 4154
BsrFI RCCGGY 2 cut(s) 94, 197
BssAI RCCGGY 2 cut(s) 94, 197
BssNAI GTATAC 1 cut(s) 3043
BssNI GRCGYC 2 cut(s) 207, 4460
BssSI CACGAG 1 cut(s) 4098
BssT1I CCWWGG 8 cut(s) 490, 1343, 1902, 2163, 2417, 2606, 3797, 4368
Bst1107I GTATAC 1 cut(s) 3043
Bst2BI CACGAG 1 cut(s) 4098
Bst6I CTCTTC 8 cut(s) 1434, 1518, 1580, 1887, 2478, 3234, 3341, 4632
BstACI GRCGYC 2 cut(s) 207, 4460
BstAPI GCANNNNNTGC 1 cut(s) 2114
BstBI TTCGAA 1 cut(s) 2431
BstDSI CCRYGG 2 cut(s) 1672, 4368
BstEII GGTNACC 1 cut(s) 4058
BstENI CCTNNNNNAGG 2 cut(s) 2034, 2580
BstFNI CGCG 5 cut(s) 26, 116, 212, 236, 3049
BstH2I RGCGCY 3 cut(s) 43, 122, 4463
BstHHI GCGC 7 cut(s) 26, 42, 116, 121, 214, 3051, 4462
BstMAI GTCTC 9 cut(s) 81, 254, 599, 1410, 3572, 3672, 4101, 4183, 4214
BstNSI RCATGY 4 cut(s) 3009, 3330, 3856, 4601
BstPAI GACNNNNGTC 3 cut(s) 2583, 2898, 3564
BstPI GGTNACC 1 cut(s) 4058
BstSFI CTRYAG 2 cut(s) 1079, 3270
BstSLI GKGCMC 2 cut(s) 4105, 4419
BstUI CGCG 5 cut(s) 26, 116, 212, 236, 3049
BstV2I GAAGAC 3 cut(s) 299, 548, 1922
BstX2I RGATCY 5 cut(s) 1599, 2203, 2299, 2413, 2972
BstXI CCANNNNNNTGG 2 cut(s) 883, 3798
BstYI RGATCY 5 cut(s) 1599, 2203, 2299, 2413, 2972
BstZ17I GTATAC 1 cut(s) 3043
Bsu15I ATCGAT 1 cut(s) 3112
Bsu36I CCTNAGG 1 cut(s) 614
BsuI GTATCC 4 cut(s) 1449, 2062, 3014, 3497
BsuTUI ATCGAT 1 cut(s) 3112
BtgI CCRYGG 2 cut(s) 1672, 4368
BtsI GCAGTG 4 cut(s) 1541, 2100, 4285, 4472
BveI ACCTGC 1 cut(s) 2845
CaiI CAGNNNCTG 1 cut(s) 1760
CciI TCATGA 3 cut(s) 460, 561, 1285
CfoI GCGC 7 cut(s) 26, 42, 116, 121, 214, 3051, 4462
Cfr10I RCCGGY 2 cut(s) 94, 197
Cfr13I GGNCC 8 cut(s) 157, 189, 2585, 2690, 3559, 3627, 3968, 4259
ClaI ATCGAT 1 cut(s) 3112
CseI GACGC 3 cut(s) 215, 242, 3845
CsiI ACCWGGT 1 cut(s) 2695
CspCI CAANNNNNGTGG 2 cut(s) 2623, 2658
DinI GGCGCC 1 cut(s) 4461
DraI TTTAAA 3 cut(s) 481, 681, 1192
EaeI YGGCCR 3 cut(s) 1224, 3907, 4522
Eam1104I CTCTTC 8 cut(s) 1434, 1518, 1580, 1887, 2478, 3234, 3341, 4632
EarI CTCTTC 8 cut(s) 1434, 1518, 1580, 1887, 2478, 3234, 3341, 4632
EciI GGCGGA 1 cut(s) 1568
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 8 cut(s) 490, 1343, 1902, 2163, 2417, 2606, 3797, 4368
Eco147I AGGCCT 1 cut(s) 2422
Eco24I GRGCYC 1 cut(s) 131
Eco31I GGTCTC 2 cut(s) 3672, 4183
Eco32I GATATC 2 cut(s) 3885, 4438
Eco47I GGWCC 5 cut(s) 157, 189, 2585, 2690, 3559
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 7 cut(s) 1392, 2372, 2881, 3284, 3365, 4145, 4169
Eco81I CCTNAGG 1 cut(s) 614
Eco88I CYCGRG 1 cut(s) 2987
Eco91I GGTNACC 1 cut(s) 4058
EcoICRI GAGCTC 1 cut(s) 129
EcoNI CCTNNNNNAGG 2 cut(s) 2034, 2580
EcoO109I RGGNCCY 3 cut(s) 2585, 3968, 4259
EcoO65I GGTNACC 1 cut(s) 4058
EcoRI GAATTC 3 cut(s) 800, 1706, 3128
EcoRV GATATC 2 cut(s) 3885, 4438
EcoT14I CCWWGG 8 cut(s) 490, 1343, 1902, 2163, 2417, 2606, 3797, 4368
EcoT22I ATGCAT 2 cut(s) 574, 4378
EcoT38I GRGCYC 1 cut(s) 131
EgeI GGCGCC 1 cut(s) 4461
EheI GGCGCC 1 cut(s) 4461
ErhI CCWWGG 8 cut(s) 490, 1343, 1902, 2163, 2417, 2606, 3797, 4368
Esp3I CGTCTC 1 cut(s) 254
FalI AAGNNNNNCTT 8 cut(s) 474, 506, 2410, 2442, 3116, 3148, 3188, 3220
FaqI GGGAC 2 cut(s) 170, 2146
FauI CCCGC 1 cut(s) 3180
FauNDI CATATG 1 cut(s) 1594
FbaI TGATCA 4 cut(s) 693, 2371, 3499, 4293
FblI GTMKAC 3 cut(s) 3042, 3269, 3859
FriOI GRGCYC 1 cut(s) 131
FspBI CTAG 6 cut(s) 554, 674, 1908, 2879, 3674, 4581
GlaI GCGC 7 cut(s) 25, 41, 115, 120, 213, 3050, 4461
GsaI CCCAGC 1 cut(s) 3055
GsuI CTGGAG 5 cut(s) 827, 1515, 3347, 3431, 3679
HaeII RGCGCY 3 cut(s) 43, 122, 4463
HapII CCGG 6 cut(s) 62, 95, 111, 198, 466, 2477
HgaI GACGC 3 cut(s) 215, 242, 3845
HhaI GCGC 7 cut(s) 26, 42, 116, 121, 214, 3051, 4462
Hin1I GRCGYC 2 cut(s) 207, 4460
Hin6I GCGC 7 cut(s) 24, 40, 114, 119, 212, 3049, 4460
HinP1I GCGC 7 cut(s) 24, 40, 114, 119, 212, 3049, 4460
HincII GTYRAC 3 cut(s) 956, 1072, 3994
HindII GTYRAC 3 cut(s) 956, 1072, 3994
HindIII AAGCTT 1 cut(s) 3914
HpaII CCGG 6 cut(s) 62, 95, 111, 198, 466, 2477
Hpy99I CGWCG 4 cut(s) 209, 236, 385, 4543
HpyCH4IV ACGT 3 cut(s) 383, 393, 3481
HpySE526I ACGT 3 cut(s) 383, 393, 3481
Hsp92I GRCGYC 2 cut(s) 207, 4460
HspAI GCGC 7 cut(s) 24, 40, 114, 119, 212, 3049, 4460
KasI GGCGCC 1 cut(s) 4459
KpnI GGTACC 1 cut(s) 3871
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Ksp22I TGATCA 4 cut(s) 693, 2371, 3499, 4293
LguI GCTCTTC 3 cut(s) 1434, 1518, 2478
LweI GCATC 9 cut(s) 463, 889, 2329, 2503, 3691, 4363, 4420, 4444, 4582
MabI ACCWGGT 1 cut(s) 2695
MaeI CTAG 6 cut(s) 554, 674, 1908, 2879, 3674, 4581
MaeII ACGT 3 cut(s) 383, 393, 3481
MaeIII GTNAC 6 cut(s) 1762, 3074, 3258, 3982, 4058, 4564
MbiI CCGCTC 1 cut(s) 2075
MfeI CAATTG 4 cut(s) 1124, 1239, 2981, 3746
MflI RGATCY 5 cut(s) 1599, 2203, 2299, 2413, 2972
MhlI GDGCHC 8 cut(s) 131, 393, 1146, 1804, 3409, 3952, 4105, 4419
MlsI TGGCCA 3 cut(s) 1226, 3909, 4524
MluNI TGGCCA 3 cut(s) 1226, 3909, 4524
Mly113I GGCGCC 1 cut(s) 4460
MlyI GAGTC 6 cut(s) 752, 961, 2522, 3176, 3574, 3791
MmeI TCCRAC 5 cut(s) 123, 2623, 2919, 3162, 4198
Mox20I TGGCCA 3 cut(s) 1226, 3909, 4524
Mph1103I ATGCAT 2 cut(s) 574, 4378
MroNI GCCGGC 1 cut(s) 197
MroXI GAANNNNTTC 4 cut(s) 227, 1994, 2361, 4486
MscI TGGCCA 3 cut(s) 1226, 3909, 4524
MslI CAYNNNNRTG 8 cut(s) 894, 1023, 1635, 2927, 3437, 4065, 4335, 4424
Msp20I TGGCCA 3 cut(s) 1226, 3909, 4524
MspA1I CMGCKG 4 cut(s) 179, 1180, 1757, 3275
MspI CCGG 6 cut(s) 62, 95, 111, 198, 466, 2477
MteI GCGCNGCGC 1 cut(s) 117
MunI CAATTG 4 cut(s) 1124, 1239, 2981, 3746
Mva1269I GAATGC 3 cut(s) 1642, 1828, 3594
MvnI CGCG 5 cut(s) 26, 116, 212, 236, 3049
NaeI GCCGGC 1 cut(s) 199
NarI GGCGCC 1 cut(s) 4460
NciI CCSGG 3 cut(s) 62, 111, 2478
NcoI CCATGG 1 cut(s) 4368
NdeI CATATG 1 cut(s) 1594
NgoMIV GCCGGC 1 cut(s) 197
NheI GCTAGC 1 cut(s) 2878
NlaIV GGNNCC 8 cut(s) 158, 300, 1900, 2415, 3869, 4260, 4461, 4605
NmeAIII GCCGAG 2 cut(s) 79, 150
NmuCI GTSAC 3 cut(s) 1762, 4058, 4564
NsiI ATGCAT 2 cut(s) 574, 4378
NspI RCATGY 4 cut(s) 3009, 3330, 3856, 4601
NspV TTCGAA 1 cut(s) 2431
OliI CACNNNNGTG 2 cut(s) 1023, 4065
PagI TCATGA 3 cut(s) 460, 561, 1285
PasI CCCWGGG 1 cut(s) 3619
PceI AGGCCT 1 cut(s) 2422
PciI ACATGT 2 cut(s) 3005, 4597
PciSI GCTCTTC 3 cut(s) 1434, 1518, 2478
PcsI WCGNNNNNNNCGW 2 cut(s) 2908, 3478
PctI GAATGC 3 cut(s) 1642, 1828, 3594
PdiI GCCGGC 1 cut(s) 199
PdmI GAANNNNTTC 4 cut(s) 227, 1994, 2361, 4486
Pfl23II CGTACG 1 cut(s) 27
PflMI CCANNNNNTGG 4 cut(s) 340, 842, 1739, 4531
PfoI TCCNGGA 1 cut(s) 804
PleI GAGTC 6 cut(s) 751, 960, 2522, 3175, 3573, 3790
PluTI GGCGCC 1 cut(s) 4463
PpsI GAGTC 6 cut(s) 751, 960, 2522, 3175, 3573, 3790
PpuMI RGGWCCY 1 cut(s) 2585
PscI ACATGT 2 cut(s) 3005, 4597
PshAI GACNNNNGTC 3 cut(s) 2583, 2898, 3564
PshBI ATTAAT 4 cut(s) 1104, 1485, 1647, 2499
Psp124BI GAGCTC 1 cut(s) 131
Psp1406I AACGTT 1 cut(s) 3481
Psp5II RGGWCCY 1 cut(s) 2585
PspEI GGTNACC 1 cut(s) 4058
PspFI CCCAGC 1 cut(s) 3051
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 8 cut(s) 158, 300, 1900, 2415, 3869, 4260, 4461, 4605
PspPI GGNCC 8 cut(s) 157, 189, 2585, 2690, 3559, 3627, 3968, 4259
PspPPI RGGWCCY 1 cut(s) 2585
PstI CTGCAG 1 cut(s) 1083
PstNI CAGNNNCTG 1 cut(s) 1760
PsuI RGATCY 5 cut(s) 1599, 2203, 2299, 2413, 2972
PvuII CAGCTG 3 cut(s) 1180, 1757, 3275
RseI CAYNNNNRTG 8 cut(s) 894, 1023, 1635, 2927, 3437, 4065, 4335, 4424
SacI GAGCTC 1 cut(s) 131
SapI GCTCTTC 3 cut(s) 1434, 1518, 2478
Sau96I GGNCC 8 cut(s) 157, 189, 2585, 2690, 3559, 3627, 3968, 4259
ScaI AGTACT 2 cut(s) 338, 2081
SchI GAGTC 6 cut(s) 752, 961, 2522, 3176, 3574, 3791
SduI GDGCHC 8 cut(s) 131, 393, 1146, 1804, 3409, 3952, 4105, 4419
SexAI ACCWGGT 1 cut(s) 2695
SfaNI GCATC 9 cut(s) 463, 889, 2329, 2503, 3691, 4363, 4420, 4444, 4582
SfcI CTRYAG 2 cut(s) 1079, 3270
SfoI GGCGCC 1 cut(s) 4461
SfuI TTCGAA 1 cut(s) 2431
SinI GGWCC 5 cut(s) 157, 189, 2585, 2690, 3559
SmiMI CAYNNNNRTG 8 cut(s) 894, 1023, 1635, 2927, 3437, 4065, 4335, 4424
SmlI CTYRAG 3 cut(s) 949, 976, 1021
SmoI CTYRAG 3 cut(s) 949, 976, 1021
SpeI ACTAGT 1 cut(s) 4580
SseBI AGGCCT 1 cut(s) 2422
SspDI GGCGCC 1 cut(s) 4459
SspI AATATT 4 cut(s) 1738, 2143, 2219, 3891
SspMI CTAG 6 cut(s) 554, 674, 1908, 2879, 3674, 4581
SstI GAGCTC 1 cut(s) 131
StuI AGGCCT 1 cut(s) 2422
StyI CCWWGG 8 cut(s) 490, 1343, 1902, 2163, 2417, 2606, 3797, 4368
TaiI ACGT 3 cut(s) 386, 396, 3484
TaqII GACCGA 1 cut(s) 2678
TatI WGTACW 4 cut(s) 336, 912, 1165, 2079
TauI GCSGC 7 cut(s) 119, 179, 204, 212, 2279, 4131, 4460
TseFI GTSAC 3 cut(s) 1762, 4058, 4564
Tsp45I GTSAC 3 cut(s) 1762, 4058, 4564
TspGWI ACGGA 9 cut(s) 30, 278, 1145, 1592, 1661, 3575, 3914, 3942, 4583
Van91I CCANNNNNTGG 4 cut(s) 340, 842, 1739, 4531
VpaK11BI GGWCC 5 cut(s) 157, 189, 2585, 2690, 3559
VspI ATTAAT 4 cut(s) 1104, 1485, 1647, 2499
XagI CCTNNNNNAGG 2 cut(s) 2034, 2580
XceI RCATGY 4 cut(s) 3009, 3330, 3856, 4601
XcmI CCANNNNNNNNNTGG 2 cut(s) 409, 4375
XmiI GTMKAC 3 cut(s) 3042, 3269, 3859
XmnI GAANNNNTTC 4 cut(s) 227, 1994, 2361, 4486
XspI CTAG 6 cut(s) 554, 674, 1908, 2879, 3674, 4581
ZrmI AGTACT 2 cut(s) 338, 2081
Zsp2I ATGCAT 2 cut(s) 574, 4378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.