MD02G1293400.v1.1

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008765
Physical Location & Seq
Reverse (-)
34883641 .. 34891172
7532 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1293400.v1.1.491

Sequence Viewer

Length: 3615 bp
ATGAATGAAGATGATCAACTTGCTGGTGCTATGCGTGTTGATGATGCTTGTGTTTTGTACTGTGAGAGGTTTATGGAATTTCTTATTGACCTCTTGAGCCAACTACCAACAAGGAGGTACTTGAGGCCACTTGTTGCTGATGTTGCTGTAGTTGCCAAATGCCACTTGAGTGCTCTGTATAGACACGAGAAGGGGAAACTCTTTGCACAGTTGGTCGACTTACTGCAGTTTTATGAAGGATTTGAGATTAATGATCATGTTGGGACACAACTGACGGATGATGAGGTGCTTCAATCTCATTATGAATGCGTCCAATCTTTCCAGCTACTTGCATTTAAAAAGGTTCCTAAGTTGCAAGAGCTTGCATTGGCTAACATTGGTTCGATTGATAAGCGCAATGATCTGTCAAAGAAATTGAATCAGGACAAACATCTGAAGCTGGTTTCAGGGGATGACCCTTGGTCCAATAGGGTTGATTTCCTTATTGAAGTCATGGTTTCATTTTTTGAAAAGCAACAGTCTCAAAAGGAAAAAATAAATGCCCTTCCTCTGTACCCAAATGAACTTATTATGTGGGACGAAAGCCTTGTGCCAAGTATCAATTACTCTGGAGAAGGTTGTCTGGCCCTTCCAAAACTGAACTTGCAATTTTTAACATTAATAAAGAAGGATATTCAGGAAGTTGTTCCACATCTTCATGCGTACATTAATAAAGAAGGAGAAACAGCTTTTCGTGGTTGGTCAAGGATGGCAGTGCCGATCAAAGAGTTTAAGATCAGTGAGGTAAAACAGCCAAATATTGGAGAAGTCAAGCCAGCTGCTGTTACAGCAGAAGTTACTTTTAGCATTTCTAGTTATAGAGGACAAATGAGATCAGAATGGAATGCTCTTAAAGAGCATGATGTCCTGTTTTTAGTTTCTATTCGCCCATCCTTTGAGCCTCTAAGTACAGAAGAGGATGGCAAGGCGAGTGTGCCACAGAGGCTTGGTCTTCAATATGTACGAGGTTGTGAAATCATCGAGATTCGTGATGAAGAGGGAACTCTAATGAACGATTATACTGGAAGAATTAAACGTGATGAGTGGAAGCCTCCAAAAGGAGAACTGAGAACTGTGACTGTTGCTTTAGATACAGCACAATACCACATGGATGTCAGCAACATTGCCGCAAAAGGCGCAGAAGATGTTTATGGGACATTCAATATACTGATGAGGAGAAAGCCTAAGGAAAATAACTTTAAAGCAATTTTGGAATCCATTAGAGATCTTATGAATGAATATTGTATTGTTCCTGACTGGTTGCACAACATTTTTCTGGGGTATGGTAATCCTTCTGCTGCACAATGGACTAATATGCCGGATCTTTTGGAAACTGTAGATTTTAAAGATACTTTCCTGGATGCAGACCATTTGAAAGAATGTTTTCCAGATGATCAGGTTTGTTTTACTAGTCCAGATGGCACAGAAAACTTGGATCCAAGGCCCCCTTTTCGAATAAAGCTTCCCAAGATGATCAGAAGCAGCACCAATGCTCTTCCAGGGAATAAGAAATCTAGTATTGTTTCTATGAGTGATGTCCCCATGGAGAATTCCGATAGTGAGAAAGAAAAAATTGCTGTTGAAGCATACACACCTCCTGACCCAGGTCCGTATCCCCAAGATCAACCAAGGAAGAACACCGTTAAATTTACACCCACTCAGGTTGGAGCAATCATCTCAGGTATTCAGCCAGGACTAACAATGGTCGTGGGTCCACCTGGTACTGGAAAAACTGATACAGCTGTCCAAATTCTGAACGTTCTCTATCATAATTGTTCGTCTCAGAGAACATTGATAATTACTCATTCGAACCAGGCTCTAAATGACCTTTTTGAGAAGATTATGCAGAGGGATGTTCCTGCACGCTATCTTCTTCGACTTGGGCAAGGAGAGCAAGAGCTAGCAACTGATCTGGACTTCAGCCGACAAGGCCGTGTCAATGCCATGCTTGTGCGGCGCTTAGAGTTGCTAAGTGAAGTGGAGAGGCTGGCTAGATCACTCCAGTTGCCTGAGGATGTAGGTTATACATGTGAAACTGCTTGGTACTTTTGGTTGCTTCATGTATATTCGCATTGGGAGCAATTCCTCGCTGCTTGTAAGGAGAATAAAGATAAAACATCATTTGTTAAAGATTGTTTTCCCTTCAAGGAGTTCTTCTCCAACGCACCGAAACCAGTCTTTACTGGTGAGTCATTTGAGAAAGATATGCGGGCAGCTAAGGGTTGCTTCCGTCATCTGAAGACCATGTTTCAAGAGCTTGAAGAGTGTAGGGCATTTGAGTTACTGAAGTCTACTGCTGATCGTGCAAATTACCTGATGACCAAGCAGGCAAAGATAGTTGCCATGACATGCACTCATGCAGCTCTAAAGAGAAAGGACTTCCTTGAGTTAGGTTTCAAGTATGACAACTTGCTCATGGAAGAAAGTGCTCAAATTTTGGAGATTGAAACTTTCATACCAATGTTGGTCCAAAGGCAGGAAGATGGTTATGCTCGGCTAAAACGCTGCATCCTGATTGGTGATCATCACCAGTTGCCTCCTGTTGTTAAGAATATAGCATTCCAAAAATACAGCCACATGGATCAGAGTCTCTTTACGAGGTTTGTCCGTTTGGGCATTCCTTATATTGAACTCAATGCCCAGGGTAGAGCCAGGCCAAGTATAGCCAAACTGTACAACTGGAGGTACAGAGATCTGGGTGACCTTCCATATGTAAAGGAGGGTGCCATATTCCATAGAGCAAATTCTGGATTCTCCTATGAATATCAGTTGGTGGATGTGCCAGATTACCATGGCAGAGGTGAGTCTACACCTTCACCTTATTTCTTCCAAAATGTGGGGGAAGCAGAATATGTGGTCAGTGTCTATATCTATATGCGTCTACTTGGGTACCCTGCAAGCAAAATCTCCATATTGACCACTTACAATGGCCAGAAGCTTTTAATCCGCGATGTTATCAACAGACGATGTGCTCCTTATGACTTCATAGGCCCTCCCAGCAAGGTTACAACAGTTGATAAATTTCAAGGCCAGCAAAATGATTTTATACTGCTATCTCTTGTGCGTACTCGCATTGTGGGTCACCTTCGTGATGTTAGAAGATTGATTGTTGCAATGTCTCGTGCCCGACTGGGTCTTTATGTGTTTTGCCGTCGTTCTTTGTTTGAACAATGCTACGAACTTCAGCCTACATTTCAACGTCTACTTCAGAGACCTGACCACCTTGCTCTTAATTTGAATGAAATTACATCAAACACTAAGCGTCACGTTGATGAAACTGGTCCTATTCATCTTGTCAGCAGTGTTGATGAGATGATTGGTATCTACCAGCAGCTTTATGAGGTAAAGTTCCATCAGTACATGCCTTATTCAGGTCGAGTCGATGAAATCCTTCCGATGGATACAGATGGGCACCATCCCATGAATACCGATATAGCTGTAACTTCTGATGGGGCACCAGCAGATAATGGCCAGCATGGAAGCAATATGGAAGAAGACACTAAAGGTGATGTCGTTGCCAATGGGCAGAACAAAGAATTTTTTTTTTTGAGAAGAAACAATTTTATTAAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

1205

Amino Acids

137.95

Weight (kDa)

6.03

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 12 - 173 2.9e-56 Intron-binding protein aquarius N-terminal
Aquarius_N_2nd PF21143 236 - 406 4.4e-56 Intron-binding protein aquarius, beta-barrel
Aquarius_N_3rd PF21144 456 - 555 6.3e-22 Intron-binding protein aquarius insert domain
AAA_11 PF13086 576 - 863 5.1e-28 AAA domain
AAA_12 PF13087 873 - 1065 7.3e-26 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2928
AccB1I GGYRCC 4 cut(s) 2762, 2928, 3450, 3493
AccB7I CCANNNNNTGG 2 cut(s) 800, 2875
AccI GTMKAC 5 cut(s) 216, 2330, 2846, 2920, 3241
AccII CGCG 1 cut(s) 2988
AciI CCGC 4 cut(s) 1167, 1993, 2248, 2986
AclI AACGTT 1 cut(s) 1797
AclWI GGATC 4 cut(s) 1368, 1469, 1482, 2628
AcoI YGGCCR 2 cut(s) 2968, 3508
AcsI RAATTY 8 cut(s) 77, 1588, 1685, 1788, 2472, 2782, 3059, 3575
AcuI CTGAAG 6 cut(s) 455, 1942, 2297, 2345, 3206, 3230
AfiI CCNNNNNNNGG 8 cut(s) 800, 1097, 1643, 1763, 2515, 2875, 3410, 3436
AflIII ACRYGT 1 cut(s) 2066
AhdI GACNNNNNGTC 1 cut(s) 460
AhlI ACTAGT 1 cut(s) 1448
AjnI CCWGG 8 cut(s) 1395, 1537, 1642, 1729, 1756, 1851, 2679, 2690
AleI CACNNNNGTG 2 cut(s) 168, 3126
Alw21I GWGCWC 3 cut(s) 175, 2470, 3013
Alw26I GTCTC 5 cut(s) 525, 1824, 2633, 3162, 3244
AlwI GGATC 4 cut(s) 1368, 1469, 1482, 2628
AlwNI CAGNNNCTG 1 cut(s) 821
AoxI GGCC 9 cut(s) 125, 624, 1481, 1969, 2693, 2968, 3028, 3067, 3508
ApeKI GCWGC 8 cut(s) 818, 1337, 1521, 2129, 2252, 2399, 2544, 3370
ApoI RAATTY 8 cut(s) 77, 1588, 1685, 1788, 2472, 2782, 3059, 3575
AseI ATTAAT 3 cut(s) 249, 659, 708
Asp700I GAANNNNTTC 3 cut(s) 684, 1422, 3429
Asp718I GGTACC 1 cut(s) 2928
AspLEI GCGC 3 cut(s) 396, 1178, 1998
AspS9I GGNCC 8 cut(s) 462, 625, 1482, 1646, 1751, 2506, 3029, 3320
AsuHPI GGTGA 8 cut(s) 2237, 2558, 2570, 2750, 2847, 2852, 3113, 3557
AsuII TTCGAA 2 cut(s) 1492, 1847
AsuNHI GCTAGC 1 cut(s) 1939
AvaII GGWCC 5 cut(s) 462, 1646, 1751, 2506, 3320
AxyI CCTNAGG 2 cut(s) 1224, 2049
BaeGI GKGCMC 3 cut(s) 3166, 3453, 3496
BaeI ACNNNNGTAYC 4 cut(s) 1767, 1800, 2912, 2945
BalI TGGCCA 2 cut(s) 2970, 3510
BamHI GGATCC 1 cut(s) 1474
BanI GGYRCC 4 cut(s) 2762, 2928, 3450, 3493
BauI CACGAG 2 cut(s) 185, 3159
BbsI GAAGAC 3 cut(s) 983, 2285, 3540
Bbv12I GWGCWC 3 cut(s) 175, 2470, 3013
BbvI GCAGC 8 cut(s) 805, 1324, 1533, 2116, 2264, 2411, 2531, 3382
BceAI ACGGC 2 cut(s) 1956, 3174
BciT130I CCWGG 8 cut(s) 1397, 1539, 1644, 1731, 1758, 1853, 2681, 2692
BciVI GTATCC 2 cut(s) 1662, 3433
BclI TGATCA 5 cut(s) 13, 253, 1432, 1512, 2560
BcoDI GTCTC 5 cut(s) 525, 1824, 2633, 3162, 3244
BcuI ACTAGT 1 cut(s) 1448
BfaI CTAG 5 cut(s) 852, 1449, 1554, 1940, 2031
BfmI CTRYAG 3 cut(s) 147, 224, 1374
BfoI RGCGCY 1 cut(s) 1999
BfuI GTATCC 2 cut(s) 1662, 3433
BglI GCCNNNNNGGC 2 cut(s) 982, 1968
BglII AGATCT 2 cut(s) 1264, 2731
Bme1390I CCNGG 8 cut(s) 1397, 1539, 1644, 1731, 1758, 1853, 2681, 2692
Bme18I GGWCC 5 cut(s) 462, 1646, 1751, 2506, 3320
BmeRI GACNNNNNGTC 1 cut(s) 460
BmgT120I GGNCC 8 cut(s) 462, 625, 1482, 1646, 1751, 2506, 3029, 3320
BmiI GGNNCC 8 cut(s) 345, 1476, 1484, 1752, 2764, 2930, 3452, 3495
BmrFI CCNGG 8 cut(s) 1397, 1539, 1644, 1731, 1758, 1853, 2681, 2692
BmrI ACTGGG 1 cut(s) 3179
BmsI GCATC 3 cut(s) 34, 1390, 2556
BmtI GCTAGC 1 cut(s) 1943
BmuI ACTGGG 1 cut(s) 3179
BoxI GACNNNNGTC 1 cut(s) 1644
BpiI GAAGAC 3 cut(s) 983, 2285, 3540
BplI GAGNNNNNCTC 2 cut(s) 2180, 2212
BpmI CTGGAG 3 cut(s) 630, 2024, 2740
Bpu10I CCTNAGC 1 cut(s) 2256
Bpu14I TTCGAA 2 cut(s) 1492, 1847
BpuEI CTTGAG 4 cut(s) 115, 142, 187, 2444
BsaBI GATNNNNATC 1 cut(s) 3359
BsaI GGTCTC 1 cut(s) 3244
BsaJI CCNNGG 9 cut(s) 458, 1478, 1538, 1581, 1642, 1667, 2679, 2680, 2830
Bsc4I CCNNNNNNNGG 8 cut(s) 800, 1097, 1643, 1763, 2515, 2875, 3410, 3436
Bse21I CCTNAGG 2 cut(s) 1224, 2049
Bse3DI GCAATG 3 cut(s) 403, 1161, 3159
Bse8I GATNNNNATC 1 cut(s) 3359
BseBI CCWGG 8 cut(s) 1397, 1539, 1644, 1731, 1758, 1853, 2681, 2692
BseDI CCNNGG 9 cut(s) 458, 1478, 1538, 1581, 1642, 1667, 2679, 2680, 2830
BseJI GATNNNNATC 1 cut(s) 3359
BseLI CCNNNNNNNGG 8 cut(s) 800, 1097, 1643, 1763, 2515, 2875, 3410, 3436
BseMI GCAATG 3 cut(s) 403, 1161, 3159
BseMII CTCAG 5 cut(s) 1097, 1712, 1731, 1835, 2040
BseRI GAGGAG 1 cut(s) 1228
BseSI GKGCMC 3 cut(s) 3166, 3453, 3496
BseXI GCAGC 8 cut(s) 805, 1324, 1533, 2116, 2264, 2411, 2531, 3382
BseYI CCCAGC 1 cut(s) 3035
BsgI GTGCAG 2 cut(s) 1323, 1884
Bsh1236I CGCG 1 cut(s) 2988
BshFI GGCC 9 cut(s) 127, 626, 1483, 1971, 2695, 2970, 3030, 3069, 3510
BshNI GGYRCC 4 cut(s) 2762, 2928, 3450, 3493
BsiHKAI GWGCWC 3 cut(s) 175, 2470, 3013
BsiSI CCGG 1 cut(s) 1358
BslFI GGGAC 4 cut(s) 277, 590, 1207, 1562
BslI CCNNNNNNNGG 8 cut(s) 800, 1097, 1643, 1763, 2515, 2875, 3410, 3436
BsmAI GTCTC 5 cut(s) 525, 1824, 2633, 3162, 3244
BsmBI CGTCTC 1 cut(s) 1824
BsmFI GGGAC 4 cut(s) 277, 590, 1207, 1562
BsmI GAATGC 4 cut(s) 311, 889, 2597, 2655
BsnI GGCC 9 cut(s) 127, 626, 1483, 1971, 2695, 2970, 3030, 3069, 3510
Bso31I GGTCTC 1 cut(s) 3244
Bsp119I TTCGAA 2 cut(s) 1492, 1847
Bsp1286I GDGCHC 6 cut(s) 175, 2470, 3013, 3166, 3453, 3496
Bsp1407I TGTACA 1 cut(s) 2712
Bsp19I CCATGG 2 cut(s) 1581, 2830
BspACI CCGC 4 cut(s) 1167, 1993, 2248, 2986
BspANI GGCC 9 cut(s) 127, 626, 1483, 1971, 2695, 2970, 3030, 3069, 3510
BspCNI CTCAG 5 cut(s) 1098, 1711, 1730, 1834, 2041
BspFNI CGCG 1 cut(s) 2988
BspLI GGNNCC 8 cut(s) 345, 1476, 1484, 1752, 2764, 2930, 3452, 3495
BspMAI CTGCAG 1 cut(s) 228
BspOI GCTAGC 1 cut(s) 1943
BspPI GGATC 4 cut(s) 1368, 1469, 1482, 2628
BspQI GCTCTTC 1 cut(s) 1539
BspT104I TTCGAA 2 cut(s) 1492, 1847
BspT107I GGYRCC 4 cut(s) 2762, 2928, 3450, 3493
BspTNI GGTCTC 1 cut(s) 3244
BsrDI GCAATG 3 cut(s) 403, 1161, 3159
BsrGI TGTACA 1 cut(s) 2712
BssECI CCNNGG 9 cut(s) 458, 1478, 1538, 1581, 1642, 1667, 2679, 2680, 2830
BssSI CACGAG 2 cut(s) 185, 3159
BssT1I CCWWGG 5 cut(s) 458, 1478, 1581, 1667, 2830
Bst2BI CACGAG 2 cut(s) 185, 3159
Bst2UI CCWGG 8 cut(s) 1397, 1539, 1644, 1731, 1758, 1853, 2681, 2692
Bst4CI ACNGT 9 cut(s) 62, 210, 519, 1114, 1120, 1375, 1681, 2712, 3052
Bst6I CTCTTC 4 cut(s) 948, 1029, 1539, 2295
BstAUI TGTACA 1 cut(s) 2712
BstBI TTCGAA 2 cut(s) 1492, 1847
BstDSI CCRYGG 2 cut(s) 1581, 2830
BstEII GGTNACC 2 cut(s) 2738, 3119
BstENI CCTNNNNNAGG 3 cut(s) 1095, 1641, 3408
BstFNI CGCG 1 cut(s) 2988
BstH2I RGCGCY 1 cut(s) 1999
BstHHI GCGC 3 cut(s) 396, 1178, 1998
BstMAI GTCTC 5 cut(s) 525, 1824, 2633, 3162, 3244
BstNI CCWGG 8 cut(s) 1397, 1539, 1644, 1731, 1758, 1853, 2681, 2692
BstNSI RCATGY 3 cut(s) 2070, 2391, 3403
BstPAI GACNNNNGTC 1 cut(s) 1644
BstPI GGTNACC 2 cut(s) 2738, 3119
BstSCI CCNGG 8 cut(s) 1395, 1537, 1642, 1729, 1756, 1851, 2679, 2690
BstSFI CTRYAG 3 cut(s) 147, 224, 1374
BstSLI GKGCMC 3 cut(s) 3166, 3453, 3496
BstUI CGCG 1 cut(s) 2988
BstV1I GCAGC 8 cut(s) 805, 1324, 1533, 2116, 2264, 2411, 2531, 3382
BstV2I GAAGAC 3 cut(s) 983, 2285, 3540
BstX2I RGATCY 4 cut(s) 1264, 1360, 1474, 2731
BstYI RGATCY 4 cut(s) 1264, 1360, 1474, 2731
Bsu36I CCTNAGG 2 cut(s) 1224, 2049
BsuI GTATCC 2 cut(s) 1662, 3433
BsuRI GGCC 9 cut(s) 127, 626, 1483, 1971, 2695, 2970, 3030, 3069, 3510
BtgI CCRYGG 2 cut(s) 1581, 2830
BtgZI GCGATG 1 cut(s) 3003
BtsI GCAGTG 2 cut(s) 759, 3346
BtsIMutI CAGTG 4 cut(s) 759, 784, 2905, 3346
CaiI CAGNNNCTG 1 cut(s) 821
CfoI GCGC 3 cut(s) 396, 1178, 1998
Cfr13I GGNCC 8 cut(s) 462, 625, 1482, 1646, 1751, 2506, 3029, 3320
CseI GACGC 3 cut(s) 298, 2906, 3290
CsiI ACCWGGT 1 cut(s) 1756
CspCI CAANNNNNGTGG 4 cut(s) 1684, 1719, 1728, 1763
DraI TTTAAA 3 cut(s) 337, 1240, 1384
DriI GACNNNNNGTC 1 cut(s) 460
EaeI YGGCCR 2 cut(s) 2968, 3508
Eam1104I CTCTTC 4 cut(s) 948, 1029, 1539, 2295
Eam1105I GACNNNNNGTC 1 cut(s) 460
EarI CTCTTC 4 cut(s) 948, 1029, 1539, 2295
Eco130I CCWWGG 5 cut(s) 458, 1478, 1581, 1667, 2830
Eco31I GGTCTC 1 cut(s) 3244
Eco47I GGWCC 5 cut(s) 462, 1646, 1751, 2506, 3320
Eco57I CTGAAG 6 cut(s) 455, 1942, 2297, 2345, 3206, 3230
Eco81I CCTNAGG 2 cut(s) 1224, 2049
Eco91I GGTNACC 2 cut(s) 2738, 3119
EcoNI CCTNNNNNAGG 3 cut(s) 1095, 1641, 3408
EcoO109I RGGNCCY 2 cut(s) 1482, 3029
EcoO65I GGTNACC 2 cut(s) 2738, 3119
EcoRI GAATTC 1 cut(s) 1588
EcoRII CCWGG 8 cut(s) 1395, 1537, 1642, 1729, 1756, 1851, 2679, 2690
EcoT14I CCWWGG 5 cut(s) 458, 1478, 1581, 1667, 2830
ErhI CCWWGG 5 cut(s) 458, 1478, 1581, 1667, 2830
Esp3I CGTCTC 1 cut(s) 1824
FalI AAGNNNNNCTT 6 cut(s) 1471, 1503, 2177, 2209, 2249, 2281
FaqI GGGAC 4 cut(s) 277, 590, 1207, 1562
FauI CCCGC 1 cut(s) 2241
FauNDI CATATG 1 cut(s) 2749
FbaI TGATCA 5 cut(s) 13, 253, 1432, 1512, 2560
FblI GTMKAC 5 cut(s) 216, 2330, 2846, 2920, 3241
FspBI CTAG 5 cut(s) 852, 1449, 1554, 1940, 2031
GlaI GCGC 3 cut(s) 395, 1177, 1997
GsaI CCCAGC 1 cut(s) 3039
GsuI CTGGAG 3 cut(s) 630, 2024, 2740
HaeII RGCGCY 1 cut(s) 1999
HaeIII GGCC 9 cut(s) 127, 626, 1483, 1971, 2695, 2970, 3030, 3069, 3510
HapII CCGG 1 cut(s) 1358
HgaI GACGC 3 cut(s) 298, 2906, 3290
HhaI GCGC 3 cut(s) 396, 1178, 1998
Hin6I GCGC 3 cut(s) 394, 1176, 1996
HinP1I GCGC 3 cut(s) 394, 1176, 1996
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 2 cut(s) 1499, 2975
HinfI GANTC 8 cut(s) 418, 1024, 1253, 2228, 2626, 2790, 2843, 3417
HpaII CCGG 1 cut(s) 1358
HphI GGTGA 8 cut(s) 2237, 2558, 2570, 2750, 2847, 2852, 3113, 3557
Hpy166II GTNNAC 6 cut(s) 217, 1754, 2331, 2847, 2921, 3242
Hpy8I GTNNAC 6 cut(s) 217, 1754, 2331, 2847, 2921, 3242
Hpy99I CGWCG 1 cut(s) 3195
HpyCH4III ACNGT 9 cut(s) 62, 210, 519, 1114, 1120, 1375, 1681, 2712, 3052
HpyCH4IV ACGT 4 cut(s) 1075, 1797, 3238, 3306
HpySE526I ACGT 4 cut(s) 1075, 1797, 3238, 3306
HspAI GCGC 3 cut(s) 394, 1176, 1996
KpnI GGTACC 1 cut(s) 2932
Ksp22I TGATCA 5 cut(s) 13, 253, 1432, 1512, 2560
LguI GCTCTTC 1 cut(s) 1539
LmnI GCTCC 3 cut(s) 1706, 2116, 3016
Lsp1109I GCAGC 8 cut(s) 805, 1324, 1533, 2116, 2264, 2411, 2531, 3382
LweI GCATC 3 cut(s) 34, 1390, 2556
MabI ACCWGGT 1 cut(s) 1756
MaeI CTAG 5 cut(s) 852, 1449, 1554, 1940, 2031
MaeII ACGT 4 cut(s) 1075, 1797, 3238, 3306
MaeIII GTNAC 9 cut(s) 823, 835, 1114, 2319, 2738, 3043, 3119, 3302, 3478
MflI RGATCY 4 cut(s) 1264, 1360, 1474, 2731
MhlI GDGCHC 6 cut(s) 175, 2470, 3013, 3166, 3453, 3496
MlsI TGGCCA 2 cut(s) 2970, 3510
MluNI TGGCCA 2 cut(s) 2970, 3510
MlyI GAGTC 4 cut(s) 2237, 2635, 2852, 3426
MmeI TCCRAC 2 cut(s) 1684, 2223
Mox20I TGGCCA 2 cut(s) 2970, 3510
MroXI GAANNNNTTC 3 cut(s) 684, 1422, 3429
MscI TGGCCA 2 cut(s) 2970, 3510
MslI CAYNNNNRTG 7 cut(s) 168, 696, 1149, 1988, 2498, 3126, 3309
Msp20I TGGCCA 2 cut(s) 2970, 3510
MspA1I CMGCKG 2 cut(s) 818, 1781
MspI CCGG 1 cut(s) 1358
MspR9I CCNGG 8 cut(s) 1397, 1539, 1644, 1731, 1758, 1853, 2681, 2692
Mva1269I GAATGC 4 cut(s) 311, 889, 2597, 2655
MvaI CCWGG 8 cut(s) 1397, 1539, 1644, 1731, 1758, 1853, 2681, 2692
MvnI CGCG 1 cut(s) 2988
NcoI CCATGG 2 cut(s) 1581, 2830
NdeI CATATG 1 cut(s) 2749
NheI GCTAGC 1 cut(s) 1939
NlaIV GGNNCC 8 cut(s) 345, 1476, 1484, 1752, 2764, 2930, 3452, 3495
NmeAIII GCCGAG 1 cut(s) 2512
NmuCI GTSAC 4 cut(s) 1114, 2738, 3119, 3302
NspI RCATGY 3 cut(s) 2070, 2391, 3403
NspV TTCGAA 2 cut(s) 1492, 1847
OliI CACNNNNGTG 2 cut(s) 168, 3126
PasI CCCWGGG 1 cut(s) 2680
PciI ACATGT 1 cut(s) 2066
PciSI GCTCTTC 1 cut(s) 1539
PctI GAATGC 4 cut(s) 311, 889, 2597, 2655
PdmI GAANNNNTTC 3 cut(s) 684, 1422, 3429
PfeI GAWTC 4 cut(s) 418, 1024, 1253, 2790
PflFI GACNNNGTC 1 cut(s) 3171
PflMI CCANNNNNTGG 2 cut(s) 800, 2875
PfoI TCCNGGA 1 cut(s) 1395
PleI GAGTC 4 cut(s) 2236, 2634, 2851, 3425
PpsI GAGTC 4 cut(s) 2236, 2634, 2851, 3425
PscI ACATGT 1 cut(s) 2066
PshAI GACNNNNGTC 1 cut(s) 1644
PshBI ATTAAT 3 cut(s) 249, 659, 708
Psp1406I AACGTT 1 cut(s) 1797
Psp6I CCWGG 8 cut(s) 1395, 1537, 1642, 1729, 1756, 1851, 2679, 2690
PspEI GGTNACC 2 cut(s) 2738, 3119
PspFI CCCAGC 1 cut(s) 3035
PspGI CCWGG 8 cut(s) 1395, 1537, 1642, 1729, 1756, 1851, 2679, 2690
PspN4I GGNNCC 8 cut(s) 345, 1476, 1484, 1752, 2764, 2930, 3452, 3495
PspPI GGNCC 8 cut(s) 462, 625, 1482, 1646, 1751, 2506, 3029, 3320
PstI CTGCAG 1 cut(s) 228
PstNI CAGNNNCTG 1 cut(s) 821
PsuI RGATCY 4 cut(s) 1264, 1360, 1474, 2731
PsyI GACNNNGTC 1 cut(s) 3171
PvuII CAGCTG 2 cut(s) 818, 1781
RseI CAYNNNNRTG 7 cut(s) 168, 696, 1149, 1988, 2498, 3126, 3309
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 1539
Sau96I GGNCC 8 cut(s) 462, 625, 1482, 1646, 1751, 2506, 3029, 3320
SchI GAGTC 4 cut(s) 2237, 2635, 2852, 3426
ScrFI CCNGG 8 cut(s) 1397, 1539, 1644, 1731, 1758, 1853, 2681, 2692
SduI GDGCHC 6 cut(s) 175, 2470, 3013, 3166, 3453, 3496
SexAI ACCWGGT 1 cut(s) 1756
SfaNI GCATC 3 cut(s) 34, 1390, 2556
SfcI CTRYAG 3 cut(s) 147, 224, 1374
SfuI TTCGAA 2 cut(s) 1492, 1847
SinI GGWCC 5 cut(s) 462, 1646, 1751, 2506, 3320
SmiMI CAYNNNNRTG 7 cut(s) 168, 696, 1149, 1988, 2498, 3126, 3309
SmlI CTYRAG 4 cut(s) 94, 121, 166, 2423
SmoI CTYRAG 4 cut(s) 94, 121, 166, 2423
SpeI ACTAGT 1 cut(s) 1448
SsiI CCGC 4 cut(s) 1167, 1993, 2248, 2986
SspI AATATT 2 cut(s) 799, 1280
SspMI CTAG 5 cut(s) 852, 1449, 1554, 1940, 2031
StyD4I CCNGG 8 cut(s) 1395, 1537, 1642, 1729, 1756, 1851, 2679, 2690
StyI CCWWGG 5 cut(s) 458, 1478, 1581, 1667, 2830
TaaI ACNGT 9 cut(s) 62, 210, 519, 1114, 1120, 1375, 1681, 2712, 3052
TaiI ACGT 4 cut(s) 1078, 1800, 3241, 3309
TaqI TCGA 8 cut(s) 216, 383, 1020, 1492, 1847, 1915, 3415, 3420
TatI WGTACW 4 cut(s) 57, 947, 2712, 3396
TauI GCSGC 2 cut(s) 1169, 1996
TfiI GAWTC 4 cut(s) 418, 1024, 1253, 2790
TscAI CASTG 4 cut(s) 759, 784, 2905, 3346
TseFI GTSAC 4 cut(s) 1114, 2738, 3119, 3302
TseI GCWGC 8 cut(s) 818, 1337, 1521, 2129, 2252, 2399, 2544, 3370
Tsp45I GTSAC 4 cut(s) 1114, 2738, 3119, 3302
TspGWI ACGGA 4 cut(s) 290, 1638, 2258, 2636
TspRI CASTG 4 cut(s) 759, 784, 2905, 3346
Tth111I GACNNNGTC 1 cut(s) 3171
Van91I CCANNNNNTGG 2 cut(s) 800, 2875
VpaK11BI GGWCC 5 cut(s) 462, 1646, 1751, 2506, 3320
VspI ATTAAT 3 cut(s) 249, 659, 708
XagI CCTNNNNNAGG 3 cut(s) 1095, 1641, 3408
XapI RAATTY 8 cut(s) 77, 1588, 1685, 1788, 2472, 2782, 3059, 3575
XceI RCATGY 3 cut(s) 2070, 2391, 3403
XmiI GTMKAC 5 cut(s) 216, 2330, 2846, 2920, 3241
XmnI GAANNNNTTC 3 cut(s) 684, 1422, 3429
XspI CTAG 5 cut(s) 852, 1449, 1554, 1940, 2031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.