Rh1CG042600

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0008765
Physical Location & Seq
Reverse (-)
7959375 .. 7975125
15751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG042600.1

Sequence Viewer

Length: 4647 bp
ATGACCAAGGTGTACGGAACAGGCGCGTACGATTTCAAGCGCCACCATGTTGCAGAGTACCCGGTGGAGCTGAACCACCAGCTCGGAGACAAGCCGGTGGTGGAGGCTAAACCCGGTGCGGCGCTGCCGAGCTCGATAACGCTGTCGGAGATTCAGAGGGACCAGCTCACTATGATTGCCGCTGCCAATTGGTCCAAAGCCGGCGGCGACGCCGCGCAGGTGAAGAAGGCGTTCGACGCGGAGCTGGTGAAGAAGATTTACGAGACGGAGTTGTGTGTGAAGGAGGGGCAGAGGAAGACGGTGCCATTGCAGAGAGTGATGATTTTGGAGGTCAGCCAGTATTTGGAGAACTATCTCTTTCCCAATTTCGATCCCGATACGGCGACGTTCGAGCACGTTATGTCCATGATTCTCATGGTCAATGAGAAGTTTCGAGAGAATGTGGCAGCTTGGGTATGCTTTCATGACTGGAAAGATGCGTTTAAAGGATTCCTTGGAAGGGTTCTTCGACTTAAATCGGAAAGAGAATTAAGTATAGCAGAGAAGACAAATTATCTAGTATTCATGATAAATGCATTTCAGAGTTTGGAAGATGAGATAGTCAGTGAGACTGTCCTTAGGTTGGCAAGTTTCCAATCCTGGCACAGTTTGTCGTATGGTCGTTTTCAGATGGAGCTAGGTTTAAATTCAGATTTGATCAAGAAATGGAGAAGGATGATAAAGAGGGAAGCTGCAAAACATGGAGAGTCTTTTAACCCCTCAACTACACTTGAAGTCCAGTTTTTGAGAAACCTCATCGAAGAATTCCTGGAGATACTTGATTCCAAAGTCTTACCGCCAAATCATGGTGTCAATGGAGAGGATCAACTTCTTGCTGCCAATGGAATGGAACACATTGATGATGCTTGTGTTTTGTACTGTGAGAGGTTTATAGAATTTCTTATTGACCTCTTGAGTCAACTACCAACAAGGAGGTACTTGAGGCCACTTGTTGCTGATGTTGCTGTTGTTCCGAAATGCCACTTGAGTGCTTTGTATAGACATGAAAAGGGGAAGCTCTTTACCCAACTTGTTGACTTGCTGCAGTTTTATGAAGGATTTGAGATTAATGATAATGTCGGGAAACAATTGACGGATGATGAAGTGCTCCAATCTCATTATGATCGAGTACAATCTTTCCAGCTGCTTGCTTTTAAAAAGATTCCTAAGTTGCAAGAGCTTGCATTGGCCAACATTGGTTCAATTGACAACCGAAATGATCTCACTAAGAGATTGTCTGTTCTTTCTCATGAAGAATTGAAGGATTTGGTTTGTTCAAAGTTGAAACTGATTTCTAAGAATGATCCTTGGTCATCCAGGGTTGATTTCCTTACTGAAGTGATGGTTTCCTTTTTCAAGAGGCAACAGTCTCAAAAAGAAAAAATAAATGCTCTTCCACTGTATCCAAATGAGCTTATCATGTGGGATGAAAGCCTTGTGCCAAGCATTAATTACTCTGGAGAAGGTTGTCTGGCTCTTCCAAAACTTAATTTACAATTTTTGACGCTGCATGATTATCTCCTGAGAAATTTTGATCTCTTCCGCCTTGAATCAACATATGAGATCCGTGAGGATATTCAGGAGGCTGTGCCACATCTGAATGCCTGCATTAATAATGAGGGAGAAACTGCTTTCCGTGGTTGGTCAAGGATGGCTGTGCCGATTAAAGAATTCAAGATCAGTGAGGTAAAACAGCCAAATATTGGAGAAGTTAAACCAGCTGCTGTGACAGCAGAAATCACTTTTAGTATATCCAGTTATAGAGCACAGGTACGATCTGAATGGAATGCTCTTAAAGAGCATGATGTTCTATTTTTACTTTCCATACGCCCTTCCTTTGAGCCTCTAAGTGCAGAAGAGGGTGCCAAGGCTAGTGTGCCTCAGAGGCTTGGTCTTCAATATGTACGGGGTTGTGAAATCATTGAGGTTCGTGATGAGGAGGGAACTCTAATGAATGATTTCACTGGAAGAATTAAACGGGATGAGTGGAAGCCTCCAAAAGGAGAGTTGAGAACTTTGACTGTTGCTTTGGATACCGCTCAGTACTACATGGATGTTAGCAACACTGCTGCAAAGGGTGCAGAAGATGTTTATGGGACATTCAATATTTTGATGAGGAGAAAGCCCAAGGAAAATAATTTCAAAGCAATTTTAGAATCCATTAGAGATCTTATGAATGAATATTGTATTGTTCCTGACTGGTTGCACAAAACATTTCTGGGGTATGGTAATCCTTCTGCCGCACAGTGGACTAATATGCCAGATCTCTTAGAAACTGTAGATTTCAAAGATACTTTCCTTGATGCAGACCATCTGAAGGAATGTTTTCCAGATGATCAGGTTTTCTTTGTTAGTCCAGATGGTATAGAAAATATGGATCCAAGGCCTCCTTTTCGAATAAAGCTCCCCAAGAAGATCAAAAGCAGCACTAATGCTCTTCCGGGCAATAAGAAAGCAAAGATTAATTCTATGAGTGATGCCCCCATTGAGGACTCTGATAGTGAGAAAGAAAAATTTGTTGTTGAGGCATACACCCCTCCTGACCCAGGTCCTTATCCGCAAGACCAGCCAAGGCAGAACTCAGTTAGATTTACACCCACTCAGGTTGGAGCAATCCTATCAGGTATTCAGCCAGGACTAACAATGGTTGTGGGTCCACCTGGTACGGGAAAGACTGATACAGCCGTGCAAATTTTGAATGTTCTGTATCATAATTGTCCATCTCAGAGAACATTGATAATTACTCACTCAAACCAGGCTCTAAATGACCTTTTTGAAAAGATAATGCAGAGGGATGTACCTGCACGCTATCTTCTTCGACTTGGGCAAGGAGAACAAGAGCTAGCAACTGATCTTGACTTCAGTCGGCAAGGCCGTGTCAATGCCATGCTTGTGAGGCGGTTGGAGTTGCTTAGTGAAGTGGAGAGGCTGGCAAGATCTCTCCAATTGCCCGAGGATGTTGGTTATACATGTGAAACTGCTGGATACTTTTGGTTGCTTCATGTATACTCGCGCTGGGAGCAATTCCTGGCTGCTTGTAACCAAAATAAAAATAAACAATCATTTGTTAAAGATCGATTTCCCTTCAAGGAATTCTTCTCCAACGCACCAAAGCCAGTTTTTACTGGTGAGTCATTTGAGAAAGATATGCGGGCAGCTAAGGGTTGCTTTCGTCATCTAAAGACTGTGTTTCAAGAGCTTGAAGAGTGTAGGGCTTTTGAGTTACTGAAGTCTACAGCTGATCGAGCAAACTACTTGATGACGAAGCAGGCAAAGATAGTTGCCATGACATGCACTCACGCAGCTCTGAAGAGGAAGGATTTTCTCCAGTTAGGTTTCAAGTACGATAACTTGCTGATGGAAGAAAGTGCTCAAATTTTGGAGATCGAAACTTTCATTCCAATGTTGCTCCAGAGGCAGGAAGATGGTTATGCACGGCTTAAACGTTGTATCCTGATTGGTGATCATCACCAGTTGCCTCCTGTTGTCAAGAATATGGCCTTTCAAAAGTATAGCCACATGGACCAGAGTCTCTTTACAAGGTTCGTCCGTTTGGGCATTCCTTATATTGAACTTAATGCTCAGGGTAGGGCCAGGCCAAGTATAGCCAAACTTTACAACTGGAGGTATAGAGAACTAGGAGACCTTCCTTATGTAAAGGAAGATGCTGTTTACAATAGAGCAAATTCTGGATTCTCCTTTGAATATCAATTGGTAGATGTACCGGATTACCATGATAGAGGTGAGTCTGCTCCATCACCTTGGTTCTACCAAAATGAGGGAGAAGCTGAATATATCGTCAGTGTGTATATCTACATGCGTCTACTTGGGTACCCTGCAAACAAGATATCAATATTGACCACTTACAATGGCCAGAAGCTATTAATCCGTGATGTTATCAACAGACGATGTGCTCCGTATGACTTCATAGGCCCTCCCAGTAAGGTTACAACAGTTGACAAATTTCAGGGTCAGCAAAATGATTTTATATTGCTCTCTCTTGTGCGTACTCGCTTTGTGGGTCACCTTCGTGATGTTAGGAGATTGATTGTTGCCATGTCTCGTGCCCGATTGGGTCTGTATGTGTTTTGCCGCCGTTCTTTGTTTGAGCAATGCTACGAACTTCAGCCTACCTTCCAACTTTTACTTCAGAGACCTGATTGCCTTGCTCTGAATTTTAATGAGACTACACCACACACAGAACGCCATGTTGAAGAAACGGGGCCTGTCCATCTTGTAAGCAGTGTTGATGAGATGATCAGTATCTACCAGCAGCTTTATGCGGTGAAGTTTCATCAGTATGTTGCTCCTTCAAGTCAATTAGTTCCATCCATGGACGCATTGGGAGAGCAAACAAGTCAGCAAACTGATATGTCTGGGCACATTCCTATGGATGCAGATATCCCTGTATCTGCTGATGGGGCGCCAGACGATACACTGCATGGAAACAATTCAGAACTGGGCGAAGAATTGGATGTCGCAAATGGCCAAAAAGTGGATTCGTCGATTGAGAATCATACAAATGGGGTGATGGAAATGGAAACTAGTCTGGATGATGCACATGTGGCTCCTGAAAGCAATTCGGATCAAGTGAAAATGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

1548

Amino Acids

177.09

Weight (kDa)

5.65

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 56 - 469 3.6e-133 Intron-binding protein aquarius N-terminal
Aquarius_N_2nd PF21143 551 - 720 7.4e-57 Intron-binding protein aquarius, beta-barrel
Aquarius_N_3rd PF21144 770 - 869 6.5e-21 Intron-binding protein aquarius insert domain
AAA_11 PF13086 882 - 1178 1.2e-28 AAA domain
AAA_12 PF13087 1187 - 1379 6.6e-27 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 208
Acc36I ACCTGC 2 cut(s) 208, 2848
Acc65I GGTACC 1 cut(s) 3870
AccB1I GGYRCC 4 cut(s) 301, 1901, 3870, 4462
AccB7I CCANNNNNTGG 4 cut(s) 343, 845, 1742, 4534
AccBSI CCGCTC 1 cut(s) 2078
AccI GTMKAC 3 cut(s) 3045, 3272, 3862
AccII CGCG 4 cut(s) 26, 215, 239, 3052
AclI AACGTT 1 cut(s) 3484
AclWI GGATC 7 cut(s) 365, 870, 1337, 1597, 2411, 2424, 4632
AcoI YGGCCR 3 cut(s) 1227, 3910, 4525
AcuI CTGAAG 7 cut(s) 1395, 2375, 2884, 3287, 3368, 4148, 4172
AcyI GRCGYC 2 cut(s) 210, 4463
AflIII ACRYGT 2 cut(s) 3008, 4600
AhlI ACTAGT 1 cut(s) 4583
AjnI CCWGG 9 cut(s) 638, 807, 1355, 2584, 2671, 2698, 2793, 3066, 3632
AjuI GAANNNNNNNTTGG 2 cut(s) 4170, 4202
AleI CACNNNNGTG 2 cut(s) 1026, 4068
Alw21I GWGCWC 6 cut(s) 134, 396, 1149, 1807, 3412, 3955
Alw26I GTCTC 9 cut(s) 81, 257, 602, 1413, 3575, 3675, 4104, 4186, 4217
AlwI GGATC 7 cut(s) 365, 870, 1337, 1597, 2411, 2424, 4632
AlwNI CAGNNNCTG 1 cut(s) 1763
Ama87I CYCGRG 1 cut(s) 2990
ArsI GACNNNNNNTTYG 2 cut(s) 2050, 2082
AseI ATTAAT 5 cut(s) 1107, 1488, 1650, 2502, 3923
Asp700I GAANNNNTTC 4 cut(s) 230, 1997, 2364, 4489
Asp718I GGTACC 1 cut(s) 3870
AspLEI GCGC 6 cut(s) 26, 42, 124, 217, 3054, 4465
AspS9I GGNCC 8 cut(s) 160, 192, 2588, 2693, 3562, 3630, 3971, 4262
AsuC2I CCSGG 3 cut(s) 62, 114, 2481
AsuII TTCGAA 1 cut(s) 2434
AsuNHI GCTAGC 1 cut(s) 2881
AvaI CYCGRG 1 cut(s) 2990
AvaII GGWCC 5 cut(s) 160, 192, 2588, 2693, 3562
AxyI CCTNAGG 1 cut(s) 617
BaeGI GKGCMC 2 cut(s) 4108, 4422
BaeI ACNNNNGTAYC 4 cut(s) 2709, 2742, 3854, 3887
BalI TGGCCA 3 cut(s) 1229, 3912, 4527
BamHI GGATCC 1 cut(s) 2416
BanI GGYRCC 4 cut(s) 301, 1901, 3870, 4462
BanII GRGCYC 1 cut(s) 134
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BauI CACGAG 1 cut(s) 4101
BbsI GAAGAC 3 cut(s) 302, 551, 1925
Bbv12I GWGCWC 6 cut(s) 134, 396, 1149, 1807, 3412, 3955
BceAI ACGGC 5 cut(s) 396, 2708, 2898, 3491, 4119
BciT130I CCWGG 9 cut(s) 640, 809, 1357, 2586, 2673, 2700, 2795, 3068, 3634
BciVI GTATCC 4 cut(s) 1452, 2065, 3017, 3500
BclI TGATCA 4 cut(s) 696, 2374, 3502, 4296
BcnI CCSGG 3 cut(s) 62, 114, 2481
BcoDI GTCTC 9 cut(s) 81, 257, 602, 1413, 3575, 3675, 4104, 4186, 4217
BcuI ACTAGT 1 cut(s) 4583
BfaI CTAG 6 cut(s) 557, 677, 1911, 2882, 3677, 4584
BfmI CTRYAG 3 cut(s) 1082, 2316, 3273
BfoI RGCGCY 3 cut(s) 43, 125, 4466
BfuAI ACCTGC 2 cut(s) 208, 2848
BfuI GTATCC 4 cut(s) 1452, 2065, 3017, 3500
BglI GCCNNNNNGGC 1 cut(s) 1924
BglII AGATCT 3 cut(s) 2206, 2302, 2975
BmcAI AGTACT 1 cut(s) 2084
Bme18I GGWCC 5 cut(s) 160, 192, 2588, 2693, 3562
BmeT110I CYCGRG 1 cut(s) 2990
BmgT120I GGNCC 8 cut(s) 160, 192, 2588, 2693, 3562, 3630, 3971, 4262
BmiI GGNNCC 9 cut(s) 161, 303, 1903, 2418, 2694, 3872, 4263, 4464, 4608
BmrI ACTGGG 2 cut(s) 3972, 4508
BmsI GCATC 7 cut(s) 466, 892, 2332, 2506, 3694, 4423, 4585
BmtI GCTAGC 1 cut(s) 2885
BmuI ACTGGG 2 cut(s) 3972, 4508
BoxI GACNNNNGTC 3 cut(s) 2586, 2901, 3567
BpiI GAAGAC 3 cut(s) 302, 551, 1925
BpmI CTGGAG 5 cut(s) 830, 1518, 3350, 3434, 3682
Bpu10I CCTNAGC 2 cut(s) 3198, 3621
Bpu14I TTCGAA 1 cut(s) 2434
BpuEI CTTGAG 3 cut(s) 973, 1000, 1045
BpuMI CCSGG 3 cut(s) 62, 114, 2481
Bsa29I ATCGAT 1 cut(s) 3115
BsaBI GATNNNNATC 1 cut(s) 4301
BsaHI GRCGYC 2 cut(s) 210, 4463
BsaI GGTCTC 2 cut(s) 3675, 4186
BsaWI WCCGGW 1 cut(s) 3763
Bse118I RCCGGY 2 cut(s) 94, 200
Bse21I CCTNAGG 1 cut(s) 617
Bse3DI GCAATG 2 cut(s) 305, 4157
Bse8I GATNNNNATC 1 cut(s) 4301
BseBI CCWGG 9 cut(s) 640, 809, 1357, 2586, 2673, 2700, 2795, 3068, 3634
BseCI ATCGAT 1 cut(s) 3115
BseJI GATNNNNATC 1 cut(s) 4301
BseMI GCAATG 2 cut(s) 305, 4157
BseMII CTCAG 7 cut(s) 1553, 1934, 2093, 2634, 2654, 2777, 3635
BseRI GAGGAG 2 cut(s) 1991, 2170
BseSI GKGCMC 2 cut(s) 4108, 4422
BseYI CCCAGC 1 cut(s) 3054
BsgI GTGCAG 3 cut(s) 1911, 2139, 2826
Bsh1236I CGCG 4 cut(s) 26, 215, 239, 3052
BshNI GGYRCC 4 cut(s) 301, 1901, 3870, 4462
BshVI ATCGAT 1 cut(s) 3115
BsiHKAI GWGCWC 6 cut(s) 134, 396, 1149, 1807, 3412, 3955
BsiHKCI CYCGRG 1 cut(s) 2990
BsiSI CCGG 6 cut(s) 62, 95, 114, 201, 2480, 3764
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 2 cut(s) 173, 2149
BsmAI GTCTC 9 cut(s) 81, 257, 602, 1413, 3575, 3675, 4104, 4186, 4217
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 2 cut(s) 173, 2149
BsmI GAATGC 3 cut(s) 1645, 1831, 3597
Bso31I GGTCTC 2 cut(s) 3675, 4186
BsoBI CYCGRG 1 cut(s) 2990
Bsp119I TTCGAA 1 cut(s) 2434
Bsp1286I GDGCHC 8 cut(s) 134, 396, 1149, 1807, 3412, 3955, 4108, 4422
Bsp19I CCATGG 1 cut(s) 4371
BspCNI CTCAG 7 cut(s) 1554, 1933, 2092, 2633, 2653, 2776, 3634
BspDI ATCGAT 1 cut(s) 3115
BspFNI CGCG 4 cut(s) 26, 215, 239, 3052
BspHI TCATGA 3 cut(s) 463, 564, 1288
BspLI GGNNCC 9 cut(s) 161, 303, 1903, 2418, 2694, 3872, 4263, 4464, 4608
BspMAI CTGCAG 1 cut(s) 1086
BspMI ACCTGC 2 cut(s) 208, 2848
BspOI GCTAGC 1 cut(s) 2885
BspPI GGATC 7 cut(s) 365, 870, 1337, 1597, 2411, 2424, 4632
BspQI GCTCTTC 3 cut(s) 1437, 1521, 2481
BspT104I TTCGAA 1 cut(s) 2434
BspT107I GGYRCC 4 cut(s) 301, 1901, 3870, 4462
BspTNI GGTCTC 2 cut(s) 3675, 4186
BsrBI CCGCTC 1 cut(s) 2078
BsrDI GCAATG 2 cut(s) 305, 4157
BsrFI RCCGGY 2 cut(s) 94, 200
BssAI RCCGGY 2 cut(s) 94, 200
BssNAI GTATAC 1 cut(s) 3046
BssNI GRCGYC 2 cut(s) 210, 4463
BssSI CACGAG 1 cut(s) 4101
BssT1I CCWWGG 9 cut(s) 6, 493, 1346, 1905, 2166, 2420, 2609, 3800, 4371
Bst1107I GTATAC 1 cut(s) 3046
Bst2BI CACGAG 1 cut(s) 4101
Bst2UI CCWGG 9 cut(s) 640, 809, 1357, 2586, 2673, 2700, 2795, 3068, 3634
Bst6I CTCTTC 8 cut(s) 1437, 1521, 1583, 1890, 2481, 3237, 3344, 4635
BstACI GRCGYC 2 cut(s) 210, 4463
BstAPI GCANNNNNTGC 1 cut(s) 2117
BstBI TTCGAA 1 cut(s) 2434
BstC8I GCNNGC 9 cut(s) 202, 1188, 1221, 1645, 2845, 2883, 2970, 3192, 3309
BstDSI CCRYGG 2 cut(s) 1675, 4371
BstEII GGTNACC 1 cut(s) 4061
BstENI CCTNNNNNAGG 2 cut(s) 2037, 2583
BstFNI CGCG 4 cut(s) 26, 215, 239, 3052
BstH2I RGCGCY 3 cut(s) 43, 125, 4466
BstHHI GCGC 6 cut(s) 26, 42, 124, 217, 3054, 4465
BstMAI GTCTC 9 cut(s) 81, 257, 602, 1413, 3575, 3675, 4104, 4186, 4217
BstNI CCWGG 9 cut(s) 640, 809, 1357, 2586, 2673, 2700, 2795, 3068, 3634
BstNSI RCATGY 4 cut(s) 3012, 3333, 3859, 4604
BstPAI GACNNNNGTC 3 cut(s) 2586, 2901, 3567
BstPI GGTNACC 1 cut(s) 4061
BstSFI CTRYAG 3 cut(s) 1082, 2316, 3273
BstSLI GKGCMC 2 cut(s) 4108, 4422
BstUI CGCG 4 cut(s) 26, 215, 239, 3052
BstV2I GAAGAC 3 cut(s) 302, 551, 1925
BstX2I RGATCY 5 cut(s) 1602, 2206, 2302, 2416, 2975
BstXI CCANNNNNNTGG 2 cut(s) 886, 3801
BstYI RGATCY 5 cut(s) 1602, 2206, 2302, 2416, 2975
BstZ17I GTATAC 1 cut(s) 3046
Bsu15I ATCGAT 1 cut(s) 3115
Bsu36I CCTNAGG 1 cut(s) 617
BsuI GTATCC 4 cut(s) 1452, 2065, 3017, 3500
BsuTUI ATCGAT 1 cut(s) 3115
BtgI CCRYGG 2 cut(s) 1675, 4371
BtsI GCAGTG 3 cut(s) 2103, 4288, 4475
BtsIMutI CAGTG 9 cut(s) 610, 1436, 1726, 2001, 2103, 2291, 3847, 4288, 4475
BveI ACCTGC 2 cut(s) 208, 2848
Cac8I GCNNGC 9 cut(s) 202, 1188, 1221, 1645, 2845, 2883, 2970, 3192, 3309
CaiI CAGNNNCTG 1 cut(s) 1763
CciI TCATGA 3 cut(s) 463, 564, 1288
CfoI GCGC 6 cut(s) 26, 42, 124, 217, 3054, 4465
Cfr10I RCCGGY 2 cut(s) 94, 200
Cfr13I GGNCC 8 cut(s) 160, 192, 2588, 2693, 3562, 3630, 3971, 4262
ClaI ATCGAT 1 cut(s) 3115
CseI GACGC 5 cut(s) 218, 245, 1552, 3848, 4385
CsiI ACCWGGT 1 cut(s) 2698
CspCI CAANNNNNGTGG 4 cut(s) 2626, 2661, 2670, 2705
DinI GGCGCC 1 cut(s) 4464
DraI TTTAAA 3 cut(s) 484, 684, 1195
EaeI YGGCCR 3 cut(s) 1227, 3910, 4525
Eam1104I CTCTTC 8 cut(s) 1437, 1521, 1583, 1890, 2481, 3237, 3344, 4635
EarI CTCTTC 8 cut(s) 1437, 1521, 1583, 1890, 2481, 3237, 3344, 4635
EciI GGCGGA 1 cut(s) 1571
Ecl136II GAGCTC 1 cut(s) 132
Eco130I CCWWGG 9 cut(s) 6, 493, 1346, 1905, 2166, 2420, 2609, 3800, 4371
Eco147I AGGCCT 1 cut(s) 2425
Eco24I GRGCYC 1 cut(s) 134
Eco31I GGTCTC 2 cut(s) 3675, 4186
Eco32I GATATC 2 cut(s) 3888, 4441
Eco47I GGWCC 5 cut(s) 160, 192, 2588, 2693, 3562
Eco53kI GAGCTC 1 cut(s) 132
Eco57I CTGAAG 7 cut(s) 1395, 2375, 2884, 3287, 3368, 4148, 4172
Eco81I CCTNAGG 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 2990
Eco91I GGTNACC 1 cut(s) 4061
EcoICRI GAGCTC 1 cut(s) 132
EcoNI CCTNNNNNAGG 2 cut(s) 2037, 2583
EcoO109I RGGNCCY 3 cut(s) 2588, 3971, 4262
EcoO65I GGTNACC 1 cut(s) 4061
EcoRI GAATTC 3 cut(s) 803, 1709, 3131
EcoRII CCWGG 9 cut(s) 638, 807, 1355, 2584, 2671, 2698, 2793, 3066, 3632
EcoRV GATATC 2 cut(s) 3888, 4441
EcoT14I CCWWGG 9 cut(s) 6, 493, 1346, 1905, 2166, 2420, 2609, 3800, 4371
EcoT22I ATGCAT 1 cut(s) 577
EcoT38I GRGCYC 1 cut(s) 134
EgeI GGCGCC 1 cut(s) 4464
EheI GGCGCC 1 cut(s) 4464
ErhI CCWWGG 9 cut(s) 6, 493, 1346, 1905, 2166, 2420, 2609, 3800, 4371
Esp3I CGTCTC 1 cut(s) 257
FalI AAGNNNNNCTT 8 cut(s) 477, 509, 2413, 2445, 3119, 3151, 3191, 3223
FaqI GGGAC 2 cut(s) 173, 2149
FauI CCCGC 1 cut(s) 3183
FauNDI CATATG 1 cut(s) 1597
FbaI TGATCA 4 cut(s) 696, 2374, 3502, 4296
FblI GTMKAC 3 cut(s) 3045, 3272, 3862
FriOI GRGCYC 1 cut(s) 134
FspBI CTAG 6 cut(s) 557, 677, 1911, 2882, 3677, 4584
GlaI GCGC 6 cut(s) 25, 41, 123, 216, 3053, 4464
GsaI CCCAGC 1 cut(s) 3058
GsuI CTGGAG 5 cut(s) 830, 1518, 3350, 3434, 3682
HaeII RGCGCY 3 cut(s) 43, 125, 4466
HapII CCGG 6 cut(s) 62, 95, 114, 201, 2480, 3764
HgaI GACGC 5 cut(s) 218, 245, 1552, 3848, 4385
HhaI GCGC 6 cut(s) 26, 42, 124, 217, 3054, 4465
Hin1I GRCGYC 2 cut(s) 210, 4463
Hin6I GCGC 6 cut(s) 24, 40, 122, 215, 3052, 4463
HinP1I GCGC 6 cut(s) 24, 40, 122, 215, 3052, 4463
HincII GTYRAC 3 cut(s) 959, 1075, 3997
HindII GTYRAC 3 cut(s) 959, 1075, 3997
HpaII CCGG 6 cut(s) 62, 95, 114, 201, 2480, 3764
Hpy99I CGWCG 4 cut(s) 212, 239, 388, 4546
HpyCH4IV ACGT 3 cut(s) 386, 396, 3484
HpySE526I ACGT 3 cut(s) 386, 396, 3484
Hsp92I GRCGYC 2 cut(s) 210, 4463
HspAI GCGC 6 cut(s) 24, 40, 122, 215, 3052, 4463
KasI GGCGCC 1 cut(s) 4462
KpnI GGTACC 1 cut(s) 3874
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Ksp22I TGATCA 4 cut(s) 696, 2374, 3502, 4296
LguI GCTCTTC 3 cut(s) 1437, 1521, 2481
LweI GCATC 7 cut(s) 466, 892, 2332, 2506, 3694, 4423, 4585
MabI ACCWGGT 1 cut(s) 2698
MaeI CTAG 6 cut(s) 557, 677, 1911, 2882, 3677, 4584
MaeII ACGT 3 cut(s) 386, 396, 3484
MaeIII GTNAC 5 cut(s) 1765, 3077, 3261, 3985, 4061
MbiI CCGCTC 1 cut(s) 2078
MfeI CAATTG 5 cut(s) 187, 1127, 1242, 2984, 3749
MflI RGATCY 5 cut(s) 1602, 2206, 2302, 2416, 2975
MhlI GDGCHC 8 cut(s) 134, 396, 1149, 1807, 3412, 3955, 4108, 4422
MlsI TGGCCA 3 cut(s) 1229, 3912, 4527
MluNI TGGCCA 3 cut(s) 1229, 3912, 4527
Mly113I GGCGCC 1 cut(s) 4463
MlyI GAGTC 6 cut(s) 755, 964, 2525, 3179, 3577, 3794
MmeI TCCRAC 5 cut(s) 126, 2626, 2922, 3165, 4201
Mox20I TGGCCA 3 cut(s) 1229, 3912, 4527
Mph1103I ATGCAT 1 cut(s) 577
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 4 cut(s) 230, 1997, 2364, 4489
MscI TGGCCA 3 cut(s) 1229, 3912, 4527
MslI CAYNNNNRTG 9 cut(s) 897, 1026, 1638, 2930, 3440, 4068, 4338, 4427, 4560
Msp20I TGGCCA 3 cut(s) 1229, 3912, 4527
MspA1I CMGCKG 4 cut(s) 182, 1183, 1760, 3278
MspI CCGG 6 cut(s) 62, 95, 114, 201, 2480, 3764
MunI CAATTG 5 cut(s) 187, 1127, 1242, 2984, 3749
Mva1269I GAATGC 3 cut(s) 1645, 1831, 3597
MvaI CCWGG 9 cut(s) 640, 809, 1357, 2586, 2673, 2700, 2795, 3068, 3634
MvnI CGCG 4 cut(s) 26, 215, 239, 3052
NaeI GCCGGC 1 cut(s) 202
NarI GGCGCC 1 cut(s) 4463
NciI CCSGG 3 cut(s) 62, 114, 2481
NcoI CCATGG 1 cut(s) 4371
NdeI CATATG 1 cut(s) 1597
NgoMIV GCCGGC 1 cut(s) 200
NheI GCTAGC 1 cut(s) 2881
NlaIV GGNNCC 9 cut(s) 161, 303, 1903, 2418, 2694, 3872, 4263, 4464, 4608
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 2 cut(s) 1765, 4061
NsiI ATGCAT 1 cut(s) 577
NspI RCATGY 4 cut(s) 3012, 3333, 3859, 4604
NspV TTCGAA 1 cut(s) 2434
OliI CACNNNNGTG 2 cut(s) 1026, 4068
PagI TCATGA 3 cut(s) 463, 564, 1288
PaqCI CACCTGC 1 cut(s) 208
PceI AGGCCT 1 cut(s) 2425
PciI ACATGT 2 cut(s) 3008, 4600
PciSI GCTCTTC 3 cut(s) 1437, 1521, 2481
PcsI WCGNNNNNNNCGW 2 cut(s) 2911, 3481
PctI GAATGC 3 cut(s) 1645, 1831, 3597
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 4 cut(s) 230, 1997, 2364, 4489
Pfl23II CGTACG 1 cut(s) 27
PflMI CCANNNNNTGG 4 cut(s) 343, 845, 1742, 4534
PfoI TCCNGGA 1 cut(s) 807
PleI GAGTC 6 cut(s) 754, 963, 2525, 3178, 3576, 3793
PluTI GGCGCC 1 cut(s) 4466
PpsI GAGTC 6 cut(s) 754, 963, 2525, 3178, 3576, 3793
PpuMI RGGWCCY 1 cut(s) 2588
PscI ACATGT 2 cut(s) 3008, 4600
PshAI GACNNNNGTC 3 cut(s) 2586, 2901, 3567
PshBI ATTAAT 5 cut(s) 1107, 1488, 1650, 2502, 3923
Psp124BI GAGCTC 1 cut(s) 134
Psp1406I AACGTT 1 cut(s) 3484
Psp5II RGGWCCY 1 cut(s) 2588
Psp6I CCWGG 9 cut(s) 638, 807, 1355, 2584, 2671, 2698, 2793, 3066, 3632
PspEI GGTNACC 1 cut(s) 4061
PspFI CCCAGC 1 cut(s) 3054
PspGI CCWGG 9 cut(s) 638, 807, 1355, 2584, 2671, 2698, 2793, 3066, 3632
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 9 cut(s) 161, 303, 1903, 2418, 2694, 3872, 4263, 4464, 4608
PspPI GGNCC 8 cut(s) 160, 192, 2588, 2693, 3562, 3630, 3971, 4262
PspPPI RGGWCCY 1 cut(s) 2588
PstI CTGCAG 1 cut(s) 1086
PstNI CAGNNNCTG 1 cut(s) 1763
PsuI RGATCY 5 cut(s) 1602, 2206, 2302, 2416, 2975
PvuII CAGCTG 3 cut(s) 1183, 1760, 3278
RseI CAYNNNNRTG 9 cut(s) 897, 1026, 1638, 2930, 3440, 4068, 4338, 4427, 4560
SacI GAGCTC 1 cut(s) 134
SapI GCTCTTC 3 cut(s) 1437, 1521, 2481
Sau96I GGNCC 8 cut(s) 160, 192, 2588, 2693, 3562, 3630, 3971, 4262
ScaI AGTACT 1 cut(s) 2084
SchI GAGTC 6 cut(s) 755, 964, 2525, 3179, 3577, 3794
SduI GDGCHC 8 cut(s) 134, 396, 1149, 1807, 3412, 3955, 4108, 4422
SexAI ACCWGGT 1 cut(s) 2698
SfaNI GCATC 7 cut(s) 466, 892, 2332, 2506, 3694, 4423, 4585
SfcI CTRYAG 3 cut(s) 1082, 2316, 3273
SfoI GGCGCC 1 cut(s) 4464
SfuI TTCGAA 1 cut(s) 2434
SinI GGWCC 5 cut(s) 160, 192, 2588, 2693, 3562
SmiMI CAYNNNNRTG 9 cut(s) 897, 1026, 1638, 2930, 3440, 4068, 4338, 4427, 4560
SmlI CTYRAG 3 cut(s) 952, 979, 1024
SmoI CTYRAG 3 cut(s) 952, 979, 1024
SpeI ACTAGT 1 cut(s) 4583
SseBI AGGCCT 1 cut(s) 2425
SspDI GGCGCC 1 cut(s) 4462
SspI AATATT 4 cut(s) 1741, 2146, 2222, 3894
SspMI CTAG 6 cut(s) 557, 677, 1911, 2882, 3677, 4584
SstI GAGCTC 1 cut(s) 134
StuI AGGCCT 1 cut(s) 2425
StyI CCWWGG 9 cut(s) 6, 493, 1346, 1905, 2166, 2420, 2609, 3800, 4371
TaiI ACGT 3 cut(s) 389, 399, 3487
TatI WGTACW 3 cut(s) 915, 1168, 2082
TauI GCSGC 6 cut(s) 122, 182, 207, 215, 2282, 4134
TscAI CASTG 9 cut(s) 610, 1443, 1726, 2008, 2110, 2291, 3847, 4288, 4482
TseFI GTSAC 2 cut(s) 1765, 4061
Tsp45I GTSAC 2 cut(s) 1765, 4061
TspGWI ACGGA 8 cut(s) 30, 281, 1148, 1595, 1664, 3578, 3917, 3945
TspRI CASTG 9 cut(s) 610, 1443, 1726, 2008, 2110, 2291, 3847, 4288, 4482
Van91I CCANNNNNTGG 4 cut(s) 343, 845, 1742, 4534
VpaK11BI GGWCC 5 cut(s) 160, 192, 2588, 2693, 3562
VspI ATTAAT 5 cut(s) 1107, 1488, 1650, 2502, 3923
XagI CCTNNNNNAGG 2 cut(s) 2037, 2583
XceI RCATGY 4 cut(s) 3012, 3333, 3859, 4604
XcmI CCANNNNNNNNNTGG 2 cut(s) 412, 4378
XmiI GTMKAC 3 cut(s) 3045, 3272, 3862
XmnI GAANNNNTTC 4 cut(s) 230, 1997, 2364, 4489
XspI CTAG 6 cut(s) 557, 677, 1911, 2882, 3677, 4584
ZrmI AGTACT 1 cut(s) 2084
Zsp2I ATGCAT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.