RLG00000030459

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008765
Physical Location & Seq
Forward (+)
61810534 .. 62024207
213674 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030459

Sequence Viewer

Length: 4602 bp
ATGACCAAGGTGTACGGAATAGGCGCGTACGATTTCAAGCGCCACCATGTTGCAGAGTACCCGGTGAAGCTGAACCAACGGCTCGGAGAGAAGCTGGTGGAGGCTAAACCCGAGGGACAGCTCACTATGATTGCCGCTGCCAATTGGTCCAAAGCTGACAGCGACACCGCACAGGCGAAGAAGGCGTTCGACGCGGACCTAGTGAAGAAGATTTACGAGACGGAGTTGTGTGTGAAGGAGGGGCAGAGGAAGATGGTGCCGTTGCAGAGAGTGATGATTTTGGAGGTCAGCCAGTACTTGGAGAACTATCTCTTTCCCAATTTCGATCTCGACATGGTGACGTTCGAGCACGTTATGTCCATGATTCTCATGGTCAATGAGAAGTTTCGAGAGAATGTGGCAGCTTGGGTATGCTTTCCTGACCGGAAAGATGCGTTTAAGGGATTCCTTGGGAGGGTTCTTCGGCTTAAATCGGAAAGAGAATTAAGTATAGCAGAGAAGACAAATTATCTAGTATTCATGATAAATGCATTTCAGAGTTTGGAAGATGAGATAGTCAGTGAGACTGTCCTTAGGTTGGCAAGTTTCCAATCCTGGCACAGTTTGTCGTATGGTCGTTTTCAGATGGAGCTAGGTTTAAATTCAGATTTGATCAAGAAATGGAGAAGGATGATAAAGAGGGAAGCTGCAAAACATGGAGAGTCTTTTAACCCCTCAACTACACTTGAAGTCCAGTTTTTGAGAAACCTCATCGAAGAATTCCTGGAGATACTTGATTCCAAAGTCTTACCGCCAAATCATGGTGTCAATGGAGAGGATCAACTTCTTGCTGCCAGTGGAATGGAACACATTGATGATGCTTGTGTTTTGTACTGTGAGAGGTTTATAGAATTTCTTATTGACCTCTTGAGTCAACTACCAACAAGGAGGTACTTGAGGCCACTTGTTGCTGATGTTGCTGTTGTTCCGAAATGCCACTTGAGTGCTTTGTATAGACATGAAAAGGGGAAGCTCTTTACCCAACTTGTTGACTTGCTGCAGTTTTATGAAGGATTTGAGATTAATGATAATGTCGGGAAACAATTGACGGATGATGAAGTGCTCCAATCTCATTATGATCGAGTACAATCTTTCCAGCTGCTTGCTTTTAAAAAGATTCCTAAGTTGCAAGAGCTTGCATTGGCCAACATTGGTTCAATTGACAACCGAAATGATCTCACTAAGAGATTGTCTGTTCTTTCTCATGAAGAATTGAAGGATTTGGTTTGTTCAAAGTTGAAACTGATTTCTAAGAATGATCCTTGGTCATCCAGGGTTGATTTCCTTACTGAAGTGATGGTTTCCTTTTTCAAGAGGCAACAGTCTCAAAAAGAAAAAATAAATGCTCTTCCACTGTATCCAAATGAGCTTATCATGTGGGATGAAAGCCTTGTGCCAAGCATTAATTACTCTGGAGAAGGTTGTCTGGCTCTTCCAAAACTTAATTTACAATTTTTGACGCTGCATGATTATCTCCTGAGAAATTTTGATCTCTTCCGCCTTGAATCAACATATGAGATCCGTGAGGATATTCAGGAGGCTGTGCCACATCTGAATGCCTGCATTAATAATGAGGGAGAAACTGCTTTCCGTGGTTGGTCAAGGATGGCTGTGCCGATTAAAGAATTCAAGATCAGTGAGGTAAAACAGCCAAATATTGGAGAAGTTAAGCCAGCTGCTGTGACAGCAGAAATCACTTTTAGTATATCCAGTTATAGAGCACAGGTACGATCAGAATGGAATGCTCTTAAAGAGCATGATGTTCTATTTTTACTTTCCATACGCCCTTCCTTTGAGCCTCTAAGTGCAGAAGAGGGTGCCAAGGCTAGTGTGCCTCAGAGGCTTGGTCTTCAATATGTACGGGGTTGTGAAATCATTGAGGTTCGTGATGAGGAGGGAACTCTAATGAATGATTTCACTGGAAGAATTAAACGGGATGAGTGGAAGCCTCCAAAAGGAGAGTTGAGAACTTTGACTGTTGCTTTGGATACCGCTCAGTACTACATGGATGTTAGCAACACTGCTGCAAAGGGTGCAGAAGATGTTTATGGGACATTCAATATTTTGATGAGGAGAAAGCCCAAGGAAAATAATTTCAAAGCAATTTTAGAATCCATTAGAGATCTTATGAATGAATATTGTATTGTTCCTGACTGGTTGCACAAAACATTTCTGGGGTATGGTAATCCTTCTGCCGCACAGTGGACTAATATGCCAGATCTCTTAGAAACCGTGGATTTCAAAGATACTTTCCTTGATGCAGACCATCTGAAGGAATGTTTTCCAGATGATCAGGTTTTCTTTGTTAGTCCAGATGGTATAGAAAATATGGATCCAAGGCCTCCTTTTCGAATAAAGCTCCCCAAGAAGATCAAAAGCAGCACTAATGCTCTTCCAGGCAATAAGAAAGCAAAGATTAATTCTATGAGTGATGCCCCCATTGAGGACTCTAATAGTGAGAAAGAAAAATTTGTTGTTGAGGCATACACCCCTCCTGACCCAGGTCCTTATCCGCAAGACCAGCCAAGGCAGAACTCAGTTAGATTTACACCCACTCAGGTTGGAGCAATCCTATCAGGTATTCAGCCAGGACTAACAATGGTTGTGGGTCCACCTGGTACGGGAAAGACTGATACAGCCGTGCAAATTTTGAATGTTCTGTATCATAATTGTCCATCTCAGAGAACATTGATAATTACTCACTCAAACCAGGCTCTAAATGACCTTTTTGAAAAGATAATGCAGAGGGATGTACCTGCACGCTATCTTCTTCGACTTGGGCAAGGAGAACAAGAGCTAGCAACTGATCTTGACTTCAGTCGGCAAGGCCGTGTCAATGCCATGCTTGTGAGGCGGTTGGAGTTGCTTAGTGAAGTGGAGAGGCTGGCAAGATCTCTCCAATTGCCCGAGGATGTTGGTTATACATGTGAAACTGCTGGATACTTTTGGTTGCTTCATGTATACTCGCGCTGGGAGCAATTCCTGGCTGCTTGTAACCAAAATAAAAATAAACAATCATTTGTTAAAGATCGATTTCCCTTCAAGGAATTCTTCTTCAACGCACCAAAGCCAGTTTTTACTGGTGAGTCATTTGAGAAAGATATGCGGGCAGCTAAGGGTTGCTTTCGTCATCTAAAGACTGTGTTTCAGGAGCTTGAAGAGTGTAGGGCTTTTGAGTTACTGAAGTCTACAGCTGATCGAGCAAACTACTTGATGACGAAGCAGGCAAAGATAGTTGCCATGACATGCACTCACGCAGCTCTGAAGAGGAAGGATTTTCTCCAGTTAGGTTTCAAGTATGATAACTTGCTGATGGAAGAAAGTGCTCAAATTTTGGAGATCGAAACTTTCATTCCAATGTTGCTCCAGAGGCAGGAAGATGGTTATGCACGGCTTAAACGTTGTATCCTGATTGGTGATCATCACCAGTTGCCTCCTGTTGTCAAGAATATGGCCTTTCAAAAGTATAGCCACATGGACCAGAGTCTCTTTACAAGGTTCGTCCGTTTGGGCATTCCTTATATTGAACTTAATGCCCAGGGTAGGGCCAGGCCAAGTATAGCCAAACTTTACAACTGGAGGTATAGAGAACTAGGAGACCTTCCTTATGTAAAGGAAGATGCTGTTTACAATAGAGCAAATTCTGGATTCTCCTTTGAATATCAATTGGTAGATGTACCAGATTACCATGATAGAGGTGAGTCTGCCCCATCACCTTGGTTCTACCAAAATGAGGGAGAAGCTGAATATATCGTCAGTGTGTATATCTACATGCGTCTACTTGGGTACCCTGCAAACAAGATATCAATATTGACCACTTACAATGGCCAGAAGCTATTAATCCGTGATGTTATCAACAGACGATGTGCTCCGTATGACTTCATAGGCCCTCCCAGTAAGGTTACAACAGTTGACAAATTTCAGGGTCAGCAAAATGATTTTATATTGCTCTCTCTTGTGCGTACTCGCTTTGTGGGTCACCTTCGTGATGTTAGGAGATTGATTGTTGCCATGTCTCGTGCCCGATTGGGTCTGTATGTGTTTTGCCGCCGTTCTTTGTTTGAGCAATGCTACGAACTTCAGCCTACCTTCCAACTTTTACTTCAGAGACCTGATCGCCTTGCTCTGAATTTTAATGAGACTACACCACACACAGAACGCCATGTTAAAGAAACGGGGCCTGTCCATCTTGTAAGCAGTGTTGATGAGATGATCAGTAGCTACCAGCAGCTTTATGCGGTGAAGTTTCATCAGTATGTTGCTCCTTCAAGTCAATTAGTTCCATCCATGGATGCATTGGGAGAGCAAACAAGTCAGCAAACTGATATGTCTGGGCACATTCCTATGGATGCAGATATCCCTGTATCTGCTGATGGGGCGCCAGACGATACACTGCATGGAAACAATTCAGAACTGGGCGAAGAATTGGATGTCGCAAATGGCCAAAAAGTGGATTCGTCGATTGAGAATCATACAAATGGGGTGACGGAAATGGAAACTAGTCTGGATGATGCACATGTGGCTCCTGAAAGCAATTCGGATCAAGTGAAAATGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

1534

Amino Acids

175.73

Weight (kDa)

5.8

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 41 - 454 4.2e-132 Intron-binding protein aquarius N-terminal
Aquarius_N_2nd PF21143 536 - 705 7.3e-57 Intron-binding protein aquarius, beta-barrel
Aquarius_N_3rd PF21144 755 - 854 5.5e-21 Intron-binding protein aquarius insert domain
AAA_11 PF13086 867 - 1163 1.4e-28 AAA domain
AAA_12 PF13087 1172 - 1364 6.5e-27 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2803
Acc65I GGTACC 1 cut(s) 3825
AccB1I GGYRCC 4 cut(s) 256, 1856, 3825, 4417
AccB7I CCANNNNNTGG 4 cut(s) 298, 800, 1697, 4489
AccBSI CCGCTC 1 cut(s) 2033
AccI GTMKAC 3 cut(s) 3000, 3227, 3817
AccII CGCG 3 cut(s) 26, 194, 3007
AclI AACGTT 1 cut(s) 3439
AclWI GGATC 6 cut(s) 825, 1292, 1552, 2366, 2379, 4587
AcoI YGGCCR 3 cut(s) 1182, 3865, 4480
AcuI CTGAAG 7 cut(s) 1350, 2330, 2839, 3242, 3323, 4103, 4127
AcyI GRCGYC 1 cut(s) 4418
AflIII ACRYGT 2 cut(s) 2963, 4555
AhlI ACTAGT 1 cut(s) 4538
AjuI GAANNNNNNNTTGG 2 cut(s) 4125, 4157
AleI CACNNNNGTG 2 cut(s) 981, 4023
Alw21I GWGCWC 5 cut(s) 351, 1104, 1762, 3367, 3910
Alw26I GTCTC 8 cut(s) 212, 557, 1368, 3530, 3630, 4059, 4141, 4172
AlwI GGATC 6 cut(s) 825, 1292, 1552, 2366, 2379, 4587
AlwNI CAGNNNCTG 1 cut(s) 1718
Ama87I CYCGRG 2 cut(s) 110, 2945
ArsI GACNNNNNNTTYG 2 cut(s) 2005, 2037
AseI ATTAAT 5 cut(s) 1062, 1443, 1605, 2457, 3878
Asp700I GAANNNNTTC 4 cut(s) 185, 1952, 2319, 4444
Asp718I GGTACC 1 cut(s) 3825
AspLEI GCGC 4 cut(s) 26, 42, 3009, 4420
AspS9I GGNCC 8 cut(s) 147, 196, 2543, 2648, 3517, 3585, 3926, 4217
AsuC2I CCSGG 1 cut(s) 62
AsuII TTCGAA 1 cut(s) 2389
AsuNHI GCTAGC 1 cut(s) 2836
AvaI CYCGRG 2 cut(s) 110, 2945
AvaII GGWCC 5 cut(s) 147, 196, 2543, 2648, 3517
AxyI CCTNAGG 1 cut(s) 572
BaeGI GKGCMC 2 cut(s) 4063, 4377
BaeI ACNNNNGTAYC 4 cut(s) 2664, 2697, 3809, 3842
BalI TGGCCA 3 cut(s) 1184, 3867, 4482
BamHI GGATCC 1 cut(s) 2371
BanI GGYRCC 4 cut(s) 256, 1856, 3825, 4417
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
BauI CACGAG 1 cut(s) 4056
BbsI GAAGAC 2 cut(s) 506, 1880
Bbv12I GWGCWC 5 cut(s) 351, 1104, 1762, 3367, 3910
BceAI ACGGC 6 cut(s) 95, 244, 2663, 2853, 3446, 4074
BciVI GTATCC 4 cut(s) 1407, 2020, 2972, 3455
BclI TGATCA 4 cut(s) 651, 2329, 3457, 4251
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 8 cut(s) 212, 557, 1368, 3530, 3630, 4059, 4141, 4172
BcuI ACTAGT 1 cut(s) 4538
BfaI CTAG 7 cut(s) 200, 512, 632, 1866, 2837, 3632, 4539
BfmI CTRYAG 2 cut(s) 1037, 3228
BfoI RGCGCY 2 cut(s) 43, 4421
BfuAI ACCTGC 1 cut(s) 2803
BfuI GTATCC 4 cut(s) 1407, 2020, 2972, 3455
BglI GCCNNNNNGGC 1 cut(s) 1879
BglII AGATCT 3 cut(s) 2161, 2257, 2930
BmcAI AGTACT 2 cut(s) 296, 2039
Bme18I GGWCC 5 cut(s) 147, 196, 2543, 2648, 3517
BmeT110I CYCGRG 2 cut(s) 110, 2945
BmgT120I GGNCC 8 cut(s) 147, 196, 2543, 2648, 3517, 3585, 3926, 4217
BmiI GGNNCC 8 cut(s) 258, 1858, 2373, 2649, 3827, 4218, 4419, 4563
BmrI ACTGGG 2 cut(s) 3927, 4463
BmsI GCATC 8 cut(s) 421, 847, 2287, 2461, 3649, 4321, 4378, 4540
BmtI GCTAGC 1 cut(s) 2840
BmuI ACTGGG 2 cut(s) 3927, 4463
BoxI GACNNNNGTC 3 cut(s) 2541, 2856, 3522
BpiI GAAGAC 2 cut(s) 506, 1880
BpmI CTGGAG 5 cut(s) 785, 1473, 3305, 3389, 3637
Bpu10I CCTNAGC 1 cut(s) 3153
Bpu14I TTCGAA 1 cut(s) 2389
BpuEI CTTGAG 3 cut(s) 928, 955, 1000
BpuMI CCSGG 1 cut(s) 62
Bsa29I ATCGAT 1 cut(s) 3070
BsaHI GRCGYC 1 cut(s) 4418
BsaI GGTCTC 2 cut(s) 3630, 4141
BsaWI WCCGGW 1 cut(s) 423
Bse21I CCTNAGG 1 cut(s) 572
Bse3DI GCAATG 1 cut(s) 4112
BseCI ATCGAT 1 cut(s) 3070
BseMI GCAATG 1 cut(s) 4112
BseMII CTCAG 6 cut(s) 1508, 1889, 2048, 2589, 2609, 2732
BseRI GAGGAG 2 cut(s) 1946, 2125
BseSI GKGCMC 2 cut(s) 4063, 4377
BseYI CCCAGC 1 cut(s) 3009
BsgI GTGCAG 3 cut(s) 1866, 2094, 2781
Bsh1236I CGCG 3 cut(s) 26, 194, 3007
BshNI GGYRCC 4 cut(s) 256, 1856, 3825, 4417
BshVI ATCGAT 1 cut(s) 3070
BsiHKAI GWGCWC 5 cut(s) 351, 1104, 1762, 3367, 3910
BsiHKCI CYCGRG 2 cut(s) 110, 2945
BsiSI CCGG 2 cut(s) 62, 424
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 2 cut(s) 129, 2104
BsmAI GTCTC 8 cut(s) 212, 557, 1368, 3530, 3630, 4059, 4141, 4172
BsmBI CGTCTC 1 cut(s) 212
BsmFI GGGAC 2 cut(s) 129, 2104
BsmI GAATGC 3 cut(s) 1600, 1786, 3552
Bso31I GGTCTC 2 cut(s) 3630, 4141
BsoBI CYCGRG 2 cut(s) 110, 2945
Bsp119I TTCGAA 1 cut(s) 2389
Bsp1286I GDGCHC 7 cut(s) 351, 1104, 1762, 3367, 3910, 4063, 4377
Bsp19I CCATGG 1 cut(s) 4326
BspCNI CTCAG 6 cut(s) 1509, 1888, 2047, 2588, 2608, 2731
BspDI ATCGAT 1 cut(s) 3070
BspFNI CGCG 3 cut(s) 26, 194, 3007
BspHI TCATGA 2 cut(s) 519, 1243
BspLI GGNNCC 8 cut(s) 258, 1858, 2373, 2649, 3827, 4218, 4419, 4563
BspMAI CTGCAG 1 cut(s) 1041
BspMI ACCTGC 1 cut(s) 2803
BspOI GCTAGC 1 cut(s) 2840
BspPI GGATC 6 cut(s) 825, 1292, 1552, 2366, 2379, 4587
BspQI GCTCTTC 3 cut(s) 1392, 1476, 2436
BspT104I TTCGAA 1 cut(s) 2389
BspT107I GGYRCC 4 cut(s) 256, 1856, 3825, 4417
BspTNI GGTCTC 2 cut(s) 3630, 4141
BsrBI CCGCTC 1 cut(s) 2033
BsrDI GCAATG 1 cut(s) 4112
BssNAI GTATAC 1 cut(s) 3001
BssNI GRCGYC 1 cut(s) 4418
BssSI CACGAG 1 cut(s) 4056
BssT1I CCWWGG 9 cut(s) 6, 448, 1301, 1860, 2121, 2375, 2564, 3755, 4326
Bst1107I GTATAC 1 cut(s) 3001
Bst2BI CACGAG 1 cut(s) 4056
Bst6I CTCTTC 8 cut(s) 1392, 1476, 1538, 1845, 2436, 3192, 3299, 4590
BstACI GRCGYC 1 cut(s) 4418
BstAPI GCANNNNNTGC 1 cut(s) 2072
BstBI TTCGAA 1 cut(s) 2389
BstC8I GCNNGC 9 cut(s) 1143, 1176, 1600, 1713, 2800, 2838, 2925, 3147, 3264
BstDSI CCRYGG 3 cut(s) 1630, 2271, 4326
BstEII GGTNACC 1 cut(s) 4016
BstENI CCTNNNNNAGG 2 cut(s) 1992, 2538
BstFNI CGCG 3 cut(s) 26, 194, 3007
BstH2I RGCGCY 2 cut(s) 43, 4421
BstHHI GCGC 4 cut(s) 26, 42, 3009, 4420
BstMAI GTCTC 8 cut(s) 212, 557, 1368, 3530, 3630, 4059, 4141, 4172
BstNSI RCATGY 4 cut(s) 2967, 3288, 3814, 4559
BstPAI GACNNNNGTC 3 cut(s) 2541, 2856, 3522
BstPI GGTNACC 1 cut(s) 4016
BstSFI CTRYAG 2 cut(s) 1037, 3228
BstSLI GKGCMC 2 cut(s) 4063, 4377
BstUI CGCG 3 cut(s) 26, 194, 3007
BstV2I GAAGAC 2 cut(s) 506, 1880
BstX2I RGATCY 5 cut(s) 1557, 2161, 2257, 2371, 2930
BstXI CCANNNNNNTGG 2 cut(s) 841, 3756
BstYI RGATCY 5 cut(s) 1557, 2161, 2257, 2371, 2930
BstZ17I GTATAC 1 cut(s) 3001
Bsu15I ATCGAT 1 cut(s) 3070
Bsu36I CCTNAGG 1 cut(s) 572
BsuI GTATCC 4 cut(s) 1407, 2020, 2972, 3455
BsuTUI ATCGAT 1 cut(s) 3070
BtgI CCRYGG 3 cut(s) 1630, 2271, 4326
BtsI GCAGTG 3 cut(s) 2058, 4243, 4430
BveI ACCTGC 1 cut(s) 2803
Cac8I GCNNGC 9 cut(s) 1143, 1176, 1600, 1713, 2800, 2838, 2925, 3147, 3264
CaiI CAGNNNCTG 1 cut(s) 1718
CciI TCATGA 2 cut(s) 519, 1243
CfoI GCGC 4 cut(s) 26, 42, 3009, 4420
Cfr13I GGNCC 8 cut(s) 147, 196, 2543, 2648, 3517, 3585, 3926, 4217
ClaI ATCGAT 1 cut(s) 3070
CseI GACGC 3 cut(s) 200, 1507, 3803
CsiI ACCWGGT 1 cut(s) 2653
CspCI CAANNNNNGTGG 4 cut(s) 2581, 2616, 2625, 2660
DinI GGCGCC 1 cut(s) 4419
DraI TTTAAA 2 cut(s) 639, 1150
EaeI YGGCCR 3 cut(s) 1182, 3865, 4480
Eam1104I CTCTTC 8 cut(s) 1392, 1476, 1538, 1845, 2436, 3192, 3299, 4590
EarI CTCTTC 8 cut(s) 1392, 1476, 1538, 1845, 2436, 3192, 3299, 4590
EciI GGCGGA 1 cut(s) 1526
Eco130I CCWWGG 9 cut(s) 6, 448, 1301, 1860, 2121, 2375, 2564, 3755, 4326
Eco147I AGGCCT 1 cut(s) 2380
Eco31I GGTCTC 2 cut(s) 3630, 4141
Eco32I GATATC 2 cut(s) 3843, 4396
Eco47I GGWCC 5 cut(s) 147, 196, 2543, 2648, 3517
Eco57I CTGAAG 7 cut(s) 1350, 2330, 2839, 3242, 3323, 4103, 4127
Eco81I CCTNAGG 1 cut(s) 572
Eco88I CYCGRG 2 cut(s) 110, 2945
Eco91I GGTNACC 1 cut(s) 4016
EcoNI CCTNNNNNAGG 2 cut(s) 1992, 2538
EcoO109I RGGNCCY 3 cut(s) 2543, 3926, 4217
EcoO65I GGTNACC 1 cut(s) 4016
EcoRI GAATTC 3 cut(s) 758, 1664, 3086
EcoRV GATATC 2 cut(s) 3843, 4396
EcoT14I CCWWGG 9 cut(s) 6, 448, 1301, 1860, 2121, 2375, 2564, 3755, 4326
EcoT22I ATGCAT 2 cut(s) 532, 4336
EgeI GGCGCC 1 cut(s) 4419
EheI GGCGCC 1 cut(s) 4419
ErhI CCWWGG 9 cut(s) 6, 448, 1301, 1860, 2121, 2375, 2564, 3755, 4326
Esp3I CGTCTC 1 cut(s) 212
FalI AAGNNNNNCTT 6 cut(s) 2368, 2400, 3074, 3106, 3146, 3178
FaqI GGGAC 2 cut(s) 129, 2104
FauI CCCGC 1 cut(s) 3138
FauNDI CATATG 1 cut(s) 1552
FbaI TGATCA 4 cut(s) 651, 2329, 3457, 4251
FblI GTMKAC 3 cut(s) 3000, 3227, 3817
FspBI CTAG 7 cut(s) 200, 512, 632, 1866, 2837, 3632, 4539
GlaI GCGC 4 cut(s) 25, 41, 3008, 4419
GsaI CCCAGC 1 cut(s) 3013
GsuI CTGGAG 5 cut(s) 785, 1473, 3305, 3389, 3637
HaeII RGCGCY 2 cut(s) 43, 4421
HapII CCGG 2 cut(s) 62, 424
HgaI GACGC 3 cut(s) 200, 1507, 3803
HhaI GCGC 4 cut(s) 26, 42, 3009, 4420
Hin1I GRCGYC 1 cut(s) 4418
Hin6I GCGC 4 cut(s) 24, 40, 3007, 4418
HinP1I GCGC 4 cut(s) 24, 40, 3007, 4418
HincII GTYRAC 3 cut(s) 914, 1030, 3952
HindII GTYRAC 3 cut(s) 914, 1030, 3952
HpaII CCGG 2 cut(s) 62, 424
Hpy99I CGWCG 2 cut(s) 194, 4501
HpyCH4IV ACGT 3 cut(s) 341, 351, 3439
HpySE526I ACGT 3 cut(s) 341, 351, 3439
Hsp92I GRCGYC 1 cut(s) 4418
HspAI GCGC 4 cut(s) 24, 40, 3007, 4418
KasI GGCGCC 1 cut(s) 4417
KpnI GGTACC 1 cut(s) 3829
Ksp22I TGATCA 4 cut(s) 651, 2329, 3457, 4251
LguI GCTCTTC 3 cut(s) 1392, 1476, 2436
LweI GCATC 8 cut(s) 421, 847, 2287, 2461, 3649, 4321, 4378, 4540
MabI ACCWGGT 1 cut(s) 2653
MaeI CTAG 7 cut(s) 200, 512, 632, 1866, 2837, 3632, 4539
MaeII ACGT 3 cut(s) 341, 351, 3439
MaeIII GTNAC 7 cut(s) 337, 1720, 3032, 3216, 3940, 4016, 4522
MbiI CCGCTC 1 cut(s) 2033
MfeI CAATTG 5 cut(s) 142, 1082, 1197, 2939, 3704
MflI RGATCY 5 cut(s) 1557, 2161, 2257, 2371, 2930
MhlI GDGCHC 7 cut(s) 351, 1104, 1762, 3367, 3910, 4063, 4377
MlsI TGGCCA 3 cut(s) 1184, 3867, 4482
MluNI TGGCCA 3 cut(s) 1184, 3867, 4482
Mly113I GGCGCC 1 cut(s) 4418
MlyI GAGTC 6 cut(s) 710, 919, 2480, 3134, 3532, 3749
MmeI TCCRAC 3 cut(s) 2581, 2877, 4156
Mox20I TGGCCA 3 cut(s) 1184, 3867, 4482
Mph1103I ATGCAT 2 cut(s) 532, 4336
MroXI GAANNNNTTC 4 cut(s) 185, 1952, 2319, 4444
MscI TGGCCA 3 cut(s) 1184, 3867, 4482
MslI CAYNNNNRTG 9 cut(s) 852, 981, 1593, 2885, 3395, 4023, 4293, 4382, 4515
Msp20I TGGCCA 3 cut(s) 1184, 3867, 4482
MspA1I CMGCKG 4 cut(s) 137, 1138, 1715, 3233
MspI CCGG 2 cut(s) 62, 424
MunI CAATTG 5 cut(s) 142, 1082, 1197, 2939, 3704
Mva1269I GAATGC 3 cut(s) 1600, 1786, 3552
MvnI CGCG 3 cut(s) 26, 194, 3007
NarI GGCGCC 1 cut(s) 4418
NciI CCSGG 1 cut(s) 62
NcoI CCATGG 1 cut(s) 4326
NdeI CATATG 1 cut(s) 1552
NheI GCTAGC 1 cut(s) 2836
NlaIV GGNNCC 8 cut(s) 258, 1858, 2373, 2649, 3827, 4218, 4419, 4563
NmuCI GTSAC 4 cut(s) 337, 1720, 4016, 4522
NsiI ATGCAT 2 cut(s) 532, 4336
NspI RCATGY 4 cut(s) 2967, 3288, 3814, 4559
NspV TTCGAA 1 cut(s) 2389
OliI CACNNNNGTG 2 cut(s) 981, 4023
PagI TCATGA 2 cut(s) 519, 1243
PasI CCCWGGG 1 cut(s) 3577
PceI AGGCCT 1 cut(s) 2380
PciI ACATGT 2 cut(s) 2963, 4555
PciSI GCTCTTC 3 cut(s) 1392, 1476, 2436
PcsI WCGNNNNNNNCGW 2 cut(s) 2866, 3436
PctI GAATGC 3 cut(s) 1600, 1786, 3552
PdmI GAANNNNTTC 4 cut(s) 185, 1952, 2319, 4444
PfeI GAWTC 9 cut(s) 364, 444, 776, 1156, 1544, 2150, 3687, 4493, 4507
Pfl23II CGTACG 1 cut(s) 27
PflMI CCANNNNNTGG 4 cut(s) 298, 800, 1697, 4489
PfoI TCCNGGA 1 cut(s) 762
PleI GAGTC 6 cut(s) 709, 918, 2480, 3133, 3531, 3748
PluTI GGCGCC 1 cut(s) 4421
PpsI GAGTC 6 cut(s) 709, 918, 2480, 3133, 3531, 3748
PpuMI RGGWCCY 1 cut(s) 2543
PscI ACATGT 2 cut(s) 2963, 4555
PshAI GACNNNNGTC 3 cut(s) 2541, 2856, 3522
PshBI ATTAAT 5 cut(s) 1062, 1443, 1605, 2457, 3878
Psp1406I AACGTT 1 cut(s) 3439
Psp5II RGGWCCY 1 cut(s) 2543
PspEI GGTNACC 1 cut(s) 4016
PspFI CCCAGC 1 cut(s) 3009
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 8 cut(s) 258, 1858, 2373, 2649, 3827, 4218, 4419, 4563
PspPI GGNCC 8 cut(s) 147, 196, 2543, 2648, 3517, 3585, 3926, 4217
PspPPI RGGWCCY 1 cut(s) 2543
PstI CTGCAG 1 cut(s) 1041
PstNI CAGNNNCTG 1 cut(s) 1718
PsuI RGATCY 5 cut(s) 1557, 2161, 2257, 2371, 2930
PvuII CAGCTG 3 cut(s) 1138, 1715, 3233
RseI CAYNNNNRTG 9 cut(s) 852, 981, 1593, 2885, 3395, 4023, 4293, 4382, 4515
SapI GCTCTTC 3 cut(s) 1392, 1476, 2436
Sau96I GGNCC 8 cut(s) 147, 196, 2543, 2648, 3517, 3585, 3926, 4217
ScaI AGTACT 2 cut(s) 296, 2039
SchI GAGTC 6 cut(s) 710, 919, 2480, 3134, 3532, 3749
SduI GDGCHC 7 cut(s) 351, 1104, 1762, 3367, 3910, 4063, 4377
SexAI ACCWGGT 1 cut(s) 2653
SfaNI GCATC 8 cut(s) 421, 847, 2287, 2461, 3649, 4321, 4378, 4540
SfcI CTRYAG 2 cut(s) 1037, 3228
SfoI GGCGCC 1 cut(s) 4419
SfuI TTCGAA 1 cut(s) 2389
SinI GGWCC 5 cut(s) 147, 196, 2543, 2648, 3517
SmiMI CAYNNNNRTG 9 cut(s) 852, 981, 1593, 2885, 3395, 4023, 4293, 4382, 4515
SmlI CTYRAG 3 cut(s) 907, 934, 979
SmoI CTYRAG 3 cut(s) 907, 934, 979
SpeI ACTAGT 1 cut(s) 4538
SseBI AGGCCT 1 cut(s) 2380
SspDI GGCGCC 1 cut(s) 4417
SspI AATATT 4 cut(s) 1696, 2101, 2177, 3849
SspMI CTAG 7 cut(s) 200, 512, 632, 1866, 2837, 3632, 4539
StuI AGGCCT 1 cut(s) 2380
StyI CCWWGG 9 cut(s) 6, 448, 1301, 1860, 2121, 2375, 2564, 3755, 4326
TaiI ACGT 3 cut(s) 344, 354, 3442
TatI WGTACW 4 cut(s) 294, 870, 1123, 2037
TauI GCSGC 3 cut(s) 137, 2237, 4089
TfiI GAWTC 9 cut(s) 364, 444, 776, 1156, 1544, 2150, 3687, 4493, 4507
TseFI GTSAC 4 cut(s) 337, 1720, 4016, 4522
Tsp45I GTSAC 4 cut(s) 337, 1720, 4016, 4522
TspGWI ACGGA 9 cut(s) 30, 236, 1103, 1550, 1619, 3533, 3872, 3900, 4541
Van91I CCANNNNNTGG 4 cut(s) 298, 800, 1697, 4489
VpaK11BI GGWCC 5 cut(s) 147, 196, 2543, 2648, 3517
VspI ATTAAT 5 cut(s) 1062, 1443, 1605, 2457, 3878
XagI CCTNNNNNAGG 2 cut(s) 1992, 2538
XceI RCATGY 4 cut(s) 2967, 3288, 3814, 4559
XcmI CCANNNNNNNNNTGG 2 cut(s) 367, 4333
XmiI GTMKAC 3 cut(s) 3000, 3227, 3817
XmnI GAANNNNTTC 4 cut(s) 185, 1952, 2319, 4444
XspI CTAG 7 cut(s) 200, 512, 632, 1866, 2837, 3632, 4539
ZrmI AGTACT 2 cut(s) 296, 2039
Zsp2I ATGCAT 2 cut(s) 532, 4336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.