pycom07g02350

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008765
Physical Location & Seq
Forward (+)
1918155 .. 1923585
5431 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g02350.1

Sequence Viewer

Length: 2367 bp
ATGACGAAGGTGTATGGAACAGGGCCGTACGATTTCAAGCGCCACCATGTCGCCGAGTACCCGCTCGAGTACCCGGTCGAGGCCAAGCCCGGTTCGGCGCTTCCTAGCTCGATAACCCTGTCGGAGATTCAGCGGGACCGGCTCACGATGATTGCCGCCGATAATTGGTCCAAAACCCTAGACACGTCGAAGCCCAAGAAGCCCTTCGAACCGGAGCTAGTGAAGGTGATTTATCGGACGGAGTTGTCAGTTGAAGAGGGTCAGAGAAGGGCCGTCCCGTCACAGAGGGTGATGATTCTGGAGGTCAGTCAGTACTTGGAGAACTATCTGTGGCCAAATTTTGACCCCGAAACCGCGACCTTCGAGCACGTTATGTCCATGATTCTCATTGTCAATGAGAAGTTTCGAGAGAATGTGGCAGCTTGGGCGTGCTTTTATGACCGGAAAGATGTGTTCAAAGGATTCCTTGAGAGGGTTCTTCGGCTTAAATCGGGAAGAGAGTTAAGCATAGCTGAGAAGACAAATTATTTAGTGTTCATGATTAATGCCTTTCAGAGTTTGGAAGATGAGATCGTGAGCGATACTGTCCTTAGGTTAGCAAGTTTGCAATCTTGGCACAGTCTGTCCTACGGTCGTTTTCAGATGGAACTTTGTTTTAATCCTGATTTGATCAGGAAATGGAAAAGGATGATCAAGAGGGAGGCCAAAGAGGCTGCAAAACGCGGAGAGCCATTTGACCCTTCAACTACACTTGAAGTTCAGTTCTTGAGAAACCTCATTGAAGAGTTCCTCGAGATACTTGATTCCAAGGTTTTAGCCCCGGATCCTATTATGAATGAAGATTATCACCTTGCTGATGCTATGCGTGTCGATGATGCTTGTGTTTTGTACTGTGAGAGGTTTATGGAATTTCTTATTGACCTCTTAAGCCAACTACCAACAAGGAGGTACTTGAGGCCGCTTGTTTCTGATGTGGCTGTAGTTGCCAAATGCCACTTGAGTGCTCTGTATAGACACGAGAAGGGGAAACTCTTTGCACAGTTGGTTGACTTGCTGCAGTTTTATGAAGGATTTGAGATTAATGATCATGTTGGGACACAACTGACAGATGATGAGGTGCTTCAATCTCATTACGAACGCGTGCAATCTTTCCAGCTACTTGCATTTAAAAAGGTTCCTAAGTTGCAAGAGCTTGCATTGGCTAACATTGGTTCGATTGACAAGCGCAATGATCTCTCAAAGAAATTGTCTGTTCTTTCTCCTGGTGAATTGAAGAATTTGGTTTGTTCAAAGCTGAAGCTGGTTTCAAGGGATGATCCATGGTCTGAAAGGGTTGATTTCCTTATTGAAGTCATGGTTTCCTTTTTTGAAAAGCAACAGTCTCAAAAGGAAAAAATAAATGCCCTTCCACTGTACCCAAATGAGCTCATCATGTGGGATGAAAGCCTTGTGCCAAGCATCAATTACTCTGGAGAAGGTTGTCTGGCCCTTCCAAAACTGAACTTACAATTTTTAACATTACATGATTATCTCCTCAGAAATTTCAATCTCTTTCGTCTCGAATCAACATATGAGATTCGTGAGGATATTCAGGAGGTTGTTCCGCATCTTCATGCATACATTAATAATGAAGGAGAAACTGCTTTTCGTGGTTGGTCAAGGATGGCAGTGCCGATCAAAGATTTTAAGATCAGCGAGGTAAAACAGCCAAATATTGGAGAAGTCAAGCCAGCTGCTGTGACAGCAGAAATTACTTTTAGCATTTCTAGTTATAGAGGACAGATGAGATCAGAATGGAATGCTCTTAAAGAGCATGATGTCCTTTTTTTACTTTCTATTCGTCCTTCCTTTGAGCCTCTAAGTGCAGAAGAGGATGGCAAGGCGAACGTGCCCCAGAGGCTTGGTCTTCAGTATGTACGGGGATGTGAAGTCATTGAGATTCGTGATGAAGAGGGAACTCTAATGAACGATTTTACTGGAAGGATTAAACGGGATGAGTGGAAGCCTCCAAAAGGAGAACTGAGAACTGTGACCGTTGCTTTAGATACAGCACAATACCACATGGATGTCAGCAACATTGCTGCAAAAGGCGCAGAAGATGTTTATGGGACATTCAATATATTGATGAGGAGAAAGCCCAAGGAAAATAACTTTAAAGCAATTTTGGAATCCATTAGAGATCTTATGAATGAATATTGTATTGTTCCGGACTGGTTGCACAACATTTTTCTGGGGTATGGTAATCCTTCTGCTGCACAATGGACTAATATGCCTGATCTTTTGGAAACTGTGGATTTTAAAGATACTTTCCTTGATGCAGACCATTTGAAAGAATGTTTTCCAGATCGATCAGGTTTGTTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

789

Amino Acids

91.22

Weight (kDa)

5.39

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 48 - 460 6.5e-137 Intron-binding protein aquarius N-terminal
Aquarius_N_2nd PF21143 541 - 711 2.2e-56 Intron-binding protein aquarius, beta-barrel
Aquarius_N_3rd PF21144 761 - 783 1.6e-06 Intron-binding protein aquarius insert domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 244
AccB7I CCANNNNNTGG 1 cut(s) 1715
AccBSI CCGCTC 1 cut(s) 64
AccII CGCG 3 cut(s) 356, 723, 1140
AccIII TCCGGA 1 cut(s) 2206
AciI CCGC 7 cut(s) 62, 133, 156, 354, 723, 959, 1604
AclWI GGATC 3 cut(s) 818, 831, 1310
AcoI YGGCCR 1 cut(s) 332
AcsI RAATTY 4 cut(s) 337, 908, 1276, 1540
AcuI CTGAAG 2 cut(s) 1316, 1892
AfaI GTAC 8 cut(s) 29, 59, 71, 314, 890, 950, 1415, 1917
AfiI CCNNNNNNNGG 5 cut(s) 79, 165, 472, 1715, 2012
AflII CTTAAG 1 cut(s) 925
AflIII ACRYGT 2 cut(s) 183, 1138
AjiI CACGTC 1 cut(s) 186
AjnI CCWGG 1 cut(s) 1261
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AleI CACNNNNGTG 1 cut(s) 999
Alw21I GWGCWC 3 cut(s) 369, 1006, 1428
Alw26I GTCTC 2 cut(s) 1386, 1562
AlwI GGATC 3 cut(s) 818, 831, 1310
AlwNI CAGNNNCTG 1 cut(s) 1736
Ama87I CYCGRG 2 cut(s) 65, 791
Aor13HI TCCGGA 1 cut(s) 2206
AoxI GGCC 7 cut(s) 23, 81, 270, 332, 702, 956, 1485
ApeKI GCWGC 6 cut(s) 419, 713, 1054, 1733, 2081, 2252
ApoI RAATTY 4 cut(s) 337, 908, 1276, 1540
ArsI GACNNNNNNTTYG 2 cut(s) 1232, 1264
AseI ATTAAT 3 cut(s) 543, 1080, 1623
Asp700I GAANNNNTTC 2 cut(s) 203, 2337
AspLEI GCGC 4 cut(s) 42, 100, 1227, 2093
AspS9I GGNCC 5 cut(s) 23, 136, 168, 270, 1486
AsuC2I CCSGG 3 cut(s) 74, 90, 821
AsuHPI GGTGA 4 cut(s) 238, 301, 839, 1277
AsuII TTCGAA 1 cut(s) 207
AvaI CYCGRG 2 cut(s) 65, 791
AvaII GGWCC 2 cut(s) 136, 168
AxyI CCTNAGG 1 cut(s) 590
BaeGI GKGCMC 1 cut(s) 1893
BalI TGGCCA 1 cut(s) 334
BamHI GGATCC 1 cut(s) 823
BanII GRGCYC 1 cut(s) 1428
BauI CACGAG 1 cut(s) 1016
BbsI GAAGAC 2 cut(s) 524, 1898
Bbv12I GWGCWC 3 cut(s) 369, 1006, 1428
BbvI GCAGC 6 cut(s) 431, 700, 1041, 1720, 2068, 2239
BccI CCATC 3 cut(s) 637, 1657, 1868
BceAI ACGGC 2 cut(s) 10, 257
BciT130I CCWGG 1 cut(s) 1263
BclI TGATCA 3 cut(s) 669, 690, 1084
BcnI CCSGG 3 cut(s) 74, 90, 821
BcoDI GTCTC 2 cut(s) 1386, 1562
BfaI CTAG 4 cut(s) 105, 179, 218, 1767
BfmI CTRYAG 2 cut(s) 978, 1055
BfoI RGCGCY 2 cut(s) 43, 101
BfrI CTTAAG 1 cut(s) 925
BglI GCCNNNNNGGC 2 cut(s) 710, 1897
BglII AGATCT 1 cut(s) 2179
BisI GCNGC 8 cut(s) 156, 420, 714, 959, 1055, 1734, 2082, 2253
BlsI GCNGC 8 cut(s) 157, 421, 715, 960, 1056, 1735, 2083, 2254
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 4 cut(s) 74, 90, 821, 1263
Bme18I GGWCC 2 cut(s) 136, 168
BmeT110I CYCGRG 2 cut(s) 65, 791
BmgBI CACGTC 1 cut(s) 186
BmgT120I GGNCC 5 cut(s) 23, 136, 168, 270, 1486
BmiI GGNNCC 3 cut(s) 137, 825, 1176
BmrFI CCNGG 4 cut(s) 74, 90, 821, 1263
BmsI GCATC 5 cut(s) 847, 865, 1467, 1615, 2305
BpiI GAAGAC 2 cut(s) 524, 1898
BpmI CTGGAG 2 cut(s) 320, 1491
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 4 cut(s) 488, 787, 973, 1018
BpuMI CCSGG 3 cut(s) 74, 90, 821
Bsa29I ATCGAT 1 cut(s) 2347
BsaJI CCNNGG 4 cut(s) 807, 819, 1319, 2139
BsaWI WCCGGW 3 cut(s) 211, 441, 2206
Bsc4I CCNNNNNNNGG 5 cut(s) 79, 165, 472, 1715, 2012
Bse118I RCCGGY 1 cut(s) 138
Bse1I ACTGG 2 cut(s) 1981, 2216
Bse21I CCTNAGG 1 cut(s) 590
Bse3DI GCAATG 2 cut(s) 1234, 2076
BseAI TCCGGA 1 cut(s) 2206
BseBI CCWGG 1 cut(s) 1263
BseCI ATCGAT 1 cut(s) 2347
BseDI CCNNGG 4 cut(s) 807, 819, 1319, 2139
BseGI GGATG 8 cut(s) 693, 1318, 1444, 1668, 1879, 1928, 1999, 2071
BseLI CCNNNNNNNGG 5 cut(s) 79, 165, 472, 1715, 2012
BseMI GCAATG 2 cut(s) 1234, 2076
BseMII CTCAG 3 cut(s) 504, 1549, 2012
BseNI ACTGG 2 cut(s) 1981, 2216
BseRI GAGGAG 2 cut(s) 1523, 2143
BseSI GKGCMC 1 cut(s) 1893
BseXI GCAGC 6 cut(s) 431, 700, 1041, 1720, 2068, 2239
BsgI GTGCAG 2 cut(s) 1884, 2238
Bsh1236I CGCG 3 cut(s) 356, 723, 1140
Bsh1285I CGRYCG 2 cut(s) 78, 634
BshFI GGCC 7 cut(s) 25, 83, 272, 334, 704, 958, 1487
BshVI ATCGAT 1 cut(s) 2347
BsiEI CGRYCG 2 cut(s) 78, 634
BsiHKAI GWGCWC 3 cut(s) 369, 1006, 1428
BsiHKCI CYCGRG 2 cut(s) 65, 791
BsiSI CCGG 7 cut(s) 74, 90, 139, 212, 442, 821, 2207
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 4 cut(s) 149, 260, 1108, 2122
BslI CCNNNNNNNGG 5 cut(s) 79, 165, 472, 1715, 2012
BsmAI GTCTC 2 cut(s) 1386, 1562
BsmBI CGTCTC 1 cut(s) 1562
BsmFI GGGAC 4 cut(s) 149, 260, 1108, 2122
BsmI GAATGC 1 cut(s) 1804
BsnI GGCC 7 cut(s) 25, 83, 272, 334, 704, 958, 1487
BsoBI CYCGRG 2 cut(s) 65, 791
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 4 cut(s) 369, 1006, 1428, 1893
Bsp13I TCCGGA 1 cut(s) 2206
Bsp19I CCATGG 1 cut(s) 1319
BspACI CCGC 7 cut(s) 62, 133, 156, 354, 723, 959, 1604
BspANI GGCC 7 cut(s) 25, 83, 272, 334, 704, 958, 1487
BspCNI CTCAG 3 cut(s) 505, 1548, 2013
BspDI ATCGAT 1 cut(s) 2347
BspEI TCCGGA 1 cut(s) 2206
BspFNI CGCG 3 cut(s) 356, 723, 1140
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 3 cut(s) 137, 825, 1176
BspMAI CTGCAG 1 cut(s) 1059
BspPI GGATC 3 cut(s) 818, 831, 1310
BspT104I TTCGAA 1 cut(s) 207
BspTI CTTAAG 1 cut(s) 925
BsrBI CCGCTC 1 cut(s) 64
BsrDI GCAATG 2 cut(s) 1234, 2076
BsrFI RCCGGY 1 cut(s) 138
BsrI ACTGG 2 cut(s) 1981, 2216
BssAI RCCGGY 1 cut(s) 138
BssECI CCNNGG 4 cut(s) 807, 819, 1319, 2139
BssSI CACGAG 1 cut(s) 1016
BssT1I CCWWGG 3 cut(s) 807, 1319, 2139
Bst2BI CACGAG 1 cut(s) 1016
Bst2UI CCWGG 1 cut(s) 1263
Bst6I CTCTTC 5 cut(s) 249, 490, 777, 1863, 1944
BstAFI CTTAAG 1 cut(s) 925
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 4 cut(s) 430, 1142, 1194, 1731
BstDEI CTNAG 6 cut(s) 513, 590, 1179, 1535, 1859, 2021
BstDSI CCRYGG 1 cut(s) 1319
BstENI CCTNNNNNAGG 1 cut(s) 2010
BstF5I GGATG 8 cut(s) 693, 1318, 1444, 1668, 1879, 1928, 1999, 2071
BstFNI CGCG 3 cut(s) 356, 723, 1140
BstH2I RGCGCY 2 cut(s) 43, 101
BstHHI GCGC 4 cut(s) 42, 100, 1227, 2093
BstMAI GTCTC 2 cut(s) 1386, 1562
BstMCI CGRYCG 2 cut(s) 78, 634
BstMWI GCNNNNNNNGC 9 cut(s) 139, 199, 425, 613, 710, 983, 1742, 1897, 2090
BstNI CCWGG 1 cut(s) 1263
BstSCI CCNGG 4 cut(s) 72, 88, 819, 1261
BstSFI CTRYAG 2 cut(s) 978, 1055
BstSLI GKGCMC 1 cut(s) 1893
BstUI CGCG 3 cut(s) 356, 723, 1140
BstV1I GCAGC 6 cut(s) 431, 700, 1041, 1720, 2068, 2239
BstV2I GAAGAC 2 cut(s) 524, 1898
BstX2I RGATCY 2 cut(s) 823, 2179
BstXI CCANNNNNNTGG 1 cut(s) 1901
BstYI RGATCY 2 cut(s) 823, 2179
Bsu15I ATCGAT 1 cut(s) 2347
Bsu36I CCTNAGG 1 cut(s) 590
BsuRI GGCC 7 cut(s) 25, 83, 272, 334, 704, 958, 1487
BsuTUI ATCGAT 1 cut(s) 2347
BtgI CCRYGG 1 cut(s) 1319
BtrI CACGTC 1 cut(s) 186
BtsCI GGATG 8 cut(s) 693, 1318, 1444, 1668, 1879, 1928, 1999, 2071
BtsI GCAGTG 1 cut(s) 1674
BtsIMutI CAGTG 2 cut(s) 1409, 1674
Cac8I GCNNGC 4 cut(s) 430, 1142, 1194, 1731
CaiI CAGNNNCTG 1 cut(s) 1736
CciI TCATGA 1 cut(s) 537
CfoI GCGC 4 cut(s) 42, 100, 1227, 2093
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 5 cut(s) 23, 136, 168, 270, 1486
ClaI ATCGAT 1 cut(s) 2347
Csp6I GTAC 8 cut(s) 28, 58, 70, 313, 889, 949, 1414, 1916
CviQI GTAC 8 cut(s) 28, 58, 70, 313, 889, 949, 1414, 1916
DdeI CTNAG 6 cut(s) 513, 590, 1179, 1535, 1859, 2021
DraI TTTAAA 3 cut(s) 1168, 2155, 2299
DrdI GACNNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 332
Eam1104I CTCTTC 5 cut(s) 249, 490, 777, 1863, 1944
EarI CTCTTC 5 cut(s) 249, 490, 777, 1863, 1944
Ecl136II GAGCTC 1 cut(s) 1426
Eco130I CCWWGG 3 cut(s) 807, 1319, 2139
Eco24I GRGCYC 1 cut(s) 1428
Eco47I GGWCC 2 cut(s) 136, 168
Eco53kI GAGCTC 1 cut(s) 1426
Eco57I CTGAAG 2 cut(s) 1316, 1892
Eco81I CCTNAGG 1 cut(s) 590
Eco88I CYCGRG 2 cut(s) 65, 791
EcoICRI GAGCTC 1 cut(s) 1426
EcoNI CCTNNNNNAGG 1 cut(s) 2010
EcoRII CCWGG 1 cut(s) 1261
EcoT14I CCWWGG 3 cut(s) 807, 1319, 2139
EcoT22I ATGCAT 1 cut(s) 1618
EcoT38I GRGCYC 1 cut(s) 1428
ErhI CCWWGG 3 cut(s) 807, 1319, 2139
Esp3I CGTCTC 1 cut(s) 1562
FalI AAGNNNNNCTT 4 cut(s) 188, 220, 450, 482
FaqI GGGAC 4 cut(s) 149, 260, 1108, 2122
FauI CCCGC 2 cut(s) 69, 126
FauNDI CATATG 1 cut(s) 1570
FbaI TGATCA 3 cut(s) 669, 690, 1084
Fnu4HI GCNGC 8 cut(s) 156, 420, 714, 959, 1055, 1734, 2082, 2253
FokI GGATG 8 cut(s) 700, 1325, 1451, 1675, 1886, 1935, 2006, 2078
FriOI GRGCYC 1 cut(s) 1428
Fsp4HI GCNGC 8 cut(s) 156, 420, 714, 959, 1055, 1734, 2082, 2253
FspBI CTAG 4 cut(s) 105, 179, 218, 1767
GlaI GCGC 4 cut(s) 41, 99, 1226, 2092
GluI GCNGC 8 cut(s) 156, 420, 714, 959, 1055, 1734, 2082, 2253
GsuI CTGGAG 2 cut(s) 320, 1491
HaeII RGCGCY 2 cut(s) 43, 101
HaeIII GGCC 7 cut(s) 25, 83, 272, 334, 704, 958, 1487
HapII CCGG 7 cut(s) 74, 90, 139, 212, 442, 821, 2207
HhaI GCGC 4 cut(s) 42, 100, 1227, 2093
Hin6I GCGC 4 cut(s) 40, 98, 1225, 2091
HinP1I GCGC 4 cut(s) 40, 98, 1225, 2091
HincII GTYRAC 1 cut(s) 1048
HindII GTYRAC 1 cut(s) 1048
HinfI GANTC 9 cut(s) 127, 295, 382, 462, 803, 1562, 1576, 1939, 2168
HpaII CCGG 7 cut(s) 74, 90, 139, 212, 442, 821, 2207
HphI GGTGA 4 cut(s) 238, 301, 839, 1277
Hpy166II GTNNAC 1 cut(s) 1048
Hpy188I TCNGA 9 cut(s) 124, 237, 264, 555, 642, 970, 1327, 1538, 1792
Hpy8I GTNNAC 1 cut(s) 1048
Hpy99I CGWCG 1 cut(s) 190
HpyCH4IV ACGT 3 cut(s) 185, 369, 1887
HpyF10VI GCNNNNNNNGC 9 cut(s) 139, 199, 425, 613, 710, 983, 1742, 1897, 2090
HpyF3I CTNAG 6 cut(s) 513, 590, 1179, 1535, 1859, 2021
HpySE526I ACGT 3 cut(s) 185, 369, 1887
HspAI GCGC 4 cut(s) 40, 98, 1225, 2091
Kpn2I TCCGGA 1 cut(s) 2206
Ksp22I TGATCA 3 cut(s) 669, 690, 1084
LmnI GCTCC 1 cut(s) 214
Lsp1109I GCAGC 6 cut(s) 431, 700, 1041, 1720, 2068, 2239
LweI GCATC 5 cut(s) 847, 865, 1467, 1615, 2305
MaeI CTAG 4 cut(s) 105, 179, 218, 1767
MaeII ACGT 3 cut(s) 185, 369, 1887
MaeIII GTNAC 3 cut(s) 279, 1738, 2029
MbiI CCGCTC 1 cut(s) 64
MflI RGATCY 2 cut(s) 823, 2179
MhlI GDGCHC 4 cut(s) 369, 1006, 1428, 1893
MlsI TGGCCA 1 cut(s) 334
MluI ACGCGT 1 cut(s) 1138
MluNI TGGCCA 1 cut(s) 334
MmeI TCCRAC 1 cut(s) 102
Mox20I TGGCCA 1 cut(s) 334
Mph1103I ATGCAT 1 cut(s) 1618
MroI TCCGGA 1 cut(s) 2206
MroXI GAANNNNTTC 2 cut(s) 203, 2337
MscI TGGCCA 1 cut(s) 334
MslI CAYNNNNRTG 3 cut(s) 999, 1611, 2064
Msp20I TGGCCA 1 cut(s) 334
MspA1I CMGCKG 2 cut(s) 133, 1733
MspCI CTTAAG 1 cut(s) 925
MspI CCGG 7 cut(s) 74, 90, 139, 212, 442, 821, 2207
MspR9I CCNGG 4 cut(s) 74, 90, 821, 1263
Mva1269I GAATGC 1 cut(s) 1804
MvaI CCWGG 1 cut(s) 1263
MvnI CGCG 3 cut(s) 356, 723, 1140
MwoI GCNNNNNNNGC 9 cut(s) 139, 199, 425, 613, 710, 983, 1742, 1897, 2090
NciI CCSGG 3 cut(s) 74, 90, 821
NcoI CCATGG 1 cut(s) 1319
NdeI CATATG 1 cut(s) 1570
NlaIV GGNNCC 3 cut(s) 137, 825, 1176
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 3 cut(s) 279, 1738, 2029
NsiI ATGCAT 1 cut(s) 1618
NspV TTCGAA 1 cut(s) 207
OliI CACNNNNGTG 1 cut(s) 999
PaeR7I CTCGAG 2 cut(s) 65, 791
PagI TCATGA 1 cut(s) 537
PctI GAATGC 1 cut(s) 1804
PdmI GAANNNNTTC 2 cut(s) 203, 2337
PfeI GAWTC 9 cut(s) 127, 295, 382, 462, 803, 1562, 1576, 1939, 2168
Pfl23II CGTACG 1 cut(s) 27
PflMI CCANNNNNTGG 1 cut(s) 1715
PkrI GCNGC 8 cut(s) 157, 421, 715, 960, 1056, 1735, 2083, 2254
PshBI ATTAAT 3 cut(s) 543, 1080, 1623
Psp124BI GAGCTC 1 cut(s) 1428
Psp6I CCWGG 1 cut(s) 1261
PspGI CCWGG 1 cut(s) 1261
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 3 cut(s) 137, 825, 1176
PspPI GGNCC 5 cut(s) 23, 136, 168, 270, 1486
PspXI VCTCGAGB 1 cut(s) 65
PstI CTGCAG 1 cut(s) 1059
PstNI CAGNNNCTG 1 cut(s) 1736
PsuI RGATCY 2 cut(s) 823, 2179
PvuII CAGCTG 1 cut(s) 1733
RsaI GTAC 8 cut(s) 29, 59, 71, 314, 890, 950, 1415, 1917
RsaNI GTAC 8 cut(s) 28, 58, 70, 313, 889, 949, 1414, 1916
RseI CAYNNNNRTG 3 cut(s) 999, 1611, 2064
SacI GAGCTC 1 cut(s) 1428
SatI GCNGC 8 cut(s) 156, 420, 714, 959, 1055, 1734, 2082, 2253
Sau96I GGNCC 5 cut(s) 23, 136, 168, 270, 1486
ScaI AGTACT 1 cut(s) 314
ScrFI CCNGG 4 cut(s) 74, 90, 821, 1263
SduI GDGCHC 4 cut(s) 369, 1006, 1428, 1893
SfaNI GCATC 5 cut(s) 847, 865, 1467, 1615, 2305
SfcI CTRYAG 2 cut(s) 978, 1055
Sfr274I CTCGAG 2 cut(s) 65, 791
SfuI TTCGAA 1 cut(s) 207
SinI GGWCC 2 cut(s) 136, 168
SlaI CTCGAG 2 cut(s) 65, 791
SmiMI CAYNNNNRTG 3 cut(s) 999, 1611, 2064
SmlI CTYRAG 7 cut(s) 65, 467, 766, 791, 925, 952, 997
SmoI CTYRAG 7 cut(s) 65, 467, 766, 791, 925, 952, 997
SsiI CCGC 7 cut(s) 62, 133, 156, 354, 723, 959, 1604
SspI AATATT 2 cut(s) 1714, 2195
SspMI CTAG 4 cut(s) 105, 179, 218, 1767
SstI GAGCTC 1 cut(s) 1428
StyD4I CCNGG 4 cut(s) 72, 88, 819, 1261
StyI CCWWGG 3 cut(s) 807, 1319, 2139
TaiI ACGT 3 cut(s) 188, 372, 1890
TatI WGTACW 2 cut(s) 312, 888
TauI GCSGC 2 cut(s) 158, 961
TfiI GAWTC 9 cut(s) 127, 295, 382, 462, 803, 1562, 1576, 1939, 2168
TscAI CASTG 2 cut(s) 1416, 1674
TseFI GTSAC 3 cut(s) 279, 1738, 2029
TseI GCWGC 6 cut(s) 419, 713, 1054, 1733, 2081, 2252
Tsp45I GTSAC 3 cut(s) 279, 1738, 2029
TspGWI ACGGA 1 cut(s) 254
TspRI CASTG 2 cut(s) 1416, 1674
Van91I CCANNNNNTGG 1 cut(s) 1715
Vha464I CTTAAG 1 cut(s) 925
VpaK11BI GGWCC 2 cut(s) 136, 168
VspI ATTAAT 3 cut(s) 543, 1080, 1623
XagI CCTNNNNNAGG 1 cut(s) 2010
XapI RAATTY 4 cut(s) 337, 908, 1276, 1540
XhoI CTCGAG 2 cut(s) 65, 791
XmnI GAANNNNTTC 2 cut(s) 203, 2337
XspI CTAG 4 cut(s) 105, 179, 218, 1767
ZrmI AGTACT 1 cut(s) 314
Zsp2I ATGCAT 1 cut(s) 1618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.