MD07G1033300.v1.1

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008765
Physical Location & Seq
Forward (+)
2742128 .. 2751800
9673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1033300.v1.1.491

Sequence Viewer

Length: 4572 bp
ATGACGAAGGTGTATGGAACAGGGCCGTACGATTTCAAGCGCCACCATGTCGCCGAGTACCCGCTCGAGTACCCGGTCGAGGCCAAGCCCGGTTCGGCGCTTCCTAGCTCGATAACGCTGTCGGAGATTCAGCGGGACCGGCTCACGATGATTGCCGCCGATAATTGGTCCAAAACCCTAGACGCCTCGAAGCCCAAGAAGCCCTTCGAACCGGAGCTAGTGAAGGTGATTTATCGGACGGAGTTGTCAGTTGAAGAGGGTCAGAGAAGGGCCGTCCCGTCACAGAGGGTGATGATTCTGGAGGTCAGTCAGTACTTGGAGAACTATCTGTGGCCAAATTTTGACCCCGAAACCGCGACCTTCGAGCACGTTATGTCCATGATTCTCATTGTCAATGAGAAGTTTCGAGAGAATGTGGCAGCTTGGGCGTGCTTTTATGACCGGAAAGATGTGTTCAAAGGATTCCTTGAGAGGGTTCTTCGGCTTAAATCGGGAAGAGAGTTAAGCATAGCTGAGAAGACAAATTATTTAGTGTTCATGATTAATGCCTTTCAGAGTTTGGAAGATGAGATCGTGAGCGATACTGTCCTTAGGTTAGCAAGTTTGCAATCTTGGCACAGTCTGTCGTACGGTCGTTTTCAGATGGAACTTTGTTTTAATCCTGATTTGATCAGGAAATGGAAAAGGATGATAAAGAGGGAGGCCAAAGACGCTGCAAAACGCGGAGAGCCATTTGACCCTTCAACTACACTTGAAGTTCAGTTCTTGAGAAACCTCATTGAAGAGTTCCTTGAGATACTTGATTCCAAGGTTTTAGCCCCTGATCCTAGTATGAATGAAGATTATCACCTTGCTGATGCTATGCGTGTCGATGATGCTTGTGTTTTGTACTGTGAGAGGTTTATGGAATTTCTTATTGACCTCTTGAGCCAACTACCAACAAGGAGGTACTTGAGGCCACTTGTTTCTGATGTGGCTGTAGTTGCCAAATGCCACTTGAGTGCTCTGTATAGACACGAGAAGGGGAAACTCTTTGCACAGTTGGTCGACTTGCTGCAGTTTTATGAAGGATTTGAGATTAATGATCATGTTGGGACACAACTGACAGATGATGAGGTGCTTCAATCTCATTACGAACGCGTGCAATCTTTCCAGCTACTTGCATTTAAAAAGGTTCCTAAGTTGCAAGAGCTTGCTTTGGCTAACATTGGTTCGATTGACAAGCGCAATGATCTCTCAAAGAAATTGTCTGTTCTTTCTCCTGGTGACTTGAAGAATTTGGTTTGTTCAAAGCTGAAGCTGGTTTCAAAGGATGATCCATGGTCTGAAAGGGTTGATTTCCTTATTGAAGTCATGGTTTCCTTTTTCGAAAAGCAACAGTCTCAAAAGGAAAAAATAAATGCCCTTCCACTGTACCCAAATGAACTCATCATGTGGGATGAAAGCCTTGTGCCAAGCATCAATTACTCTGGAGAAGGTTGTCTGGCCCTTCCAAAACTGAACTTGCAATTTTTAACATTACATGATTATCTCCTCAGAAATTTCAATCTCTTTCGTCTCGAATCAACATATGAGATTCGTGAGGATATTCAGGAAGTTGTTCCGCATCTGCATGCGTACATTAATAATGAAGGAGAAACTGCTTTTCGTGGTTGGTCAAGGATGGCAGTGCCAATCAAAGATTTTAAGATCAGTGAGGTAAAACAGCCAAATATTGGAGAAGTCAAGCCAGCTGCTGTGACAGCACAAATTACTTTTAGCATTTCTAGTTATAGAGGACAGATGAGATCAGAATGGAATGCTCTTAAAGAGCATGATGTCCTTTTTTTACTTTCTATTCGTCCTTCCTTTGAGCCTCTAAGTGCAGAAGAGGATGGCAAGGCGAGCGTGCCCCAGAGGCTTGGTCTTCAGTATGTACGGGGTTGTGAAGTCATTGAGATTCGTGATGAAGAGGGAACTCTAATGAACGATTTTACTGGAAGGATTAAACGGGATGAGTGGAAGCCTCCAAAAGGAGAACTGAGAACTGTGACCGTTGCTTTAGATACAGCACAATACCACATGGATGTCAGCAACATTGCTGCAAAAGGCGCAGAAGATGTTTATGGGACATTCAATATATTGATGAGGAGAAAGCCCAAGGAAAATAACTTTAAAGCAATTTTGGAATCCATTAGAGATCTTATGAATGAATATTGTATTGTTCCGGACTGGTTGCACAACATTTTTCTGGGGTATGGTAATCCTTCTGCTGCACAATGGACTAATATGCCGGATCTTTTGGAAACTGTGGATTTTAAAGATACTTTCCTTGATGCAGACCATTTGAAAGAATGTTTTCCAGATGATCAGGTTTGTTTTATTAGTCCAGATGGCACAGAAAACTTGAATCCAAGGCCTCCTTTTCGAATAACACTTCCCAAGACAATGAGAAGCAACACCAATGCTCTTCCAGGGAATAAGAAATCTACTAATGATGTCCCCATGGATAATTCCGATAGTGAGAAAGAAAAAATTGTTGTTGAAGCATACACACCTCCTGACCCAGGTCCGTATCCCCAAGATCAGCCAAGGAAGAACTCTGTTAAATTTACACCCACTCAGGTCGGAGCAATCATCTCAGGTATTCAGCCAGGACTAACAATGGTCGTGGGTCCACCTGGTACAGGAAAAACTGATACCGCTGTCCAAATTCTGAATGTTCTTTATCATAATTGTCCGTCTCAGAGAACATTGATAATTACTCATTCAAACCAGGCTCTAAATGACCTTTTTGAGAAGATTATGCAGAGGGATGTGCCTGCACGCTATCTTCTTCGACTTGGGCAAGGAGAGCAAGAGCTAGCAACTGATCTGGACTTCAGCCGACAAGGCCGTGTCAATGCCATGCTTGTGCGGCGCTTAGAGTTGCTAAGTGAAGTGGAGAGGCTGGCTAGATCACTCCAGTTGCCTGAGGATGTAGGTTATACATGTGAAACTGCTGGGTACTTTTGGTTGCTTCATGTTTACTCGCATTGGGAGCAATTCCTTGCTGCTTGTAAGGAGAATAAAGATAAAACGTCATTTGTTAAAGATCGTTTTCCCTTCAAGGAGTTCTTCTCCAACACACCGAAACCAGTCTTTACTGGTGAGTCATTTGAGAAAGATATGCGGGCAGCCAAGGGTTGCTTTCGTCATCTGAAGACCATGTTTCAAGAGCTTGAAGAGTGTAGGGCGTTTGAGTTACTGAAGTCTACTGCTGATCGAGCAAATTACCTGATGACCAAGCAGGCAAAGATAGTTGCCATGACATGCACTCATGCAGCTCTAAAGAGAAAGGATTTCCTTGAGTTAGGTTTCAAGTATGACAACTTGCTCATGGAAGAAAGTGCTCAAATTTTGGAGATTGAAACTTTCATACCAATGTTGCTCCAGAGGCAGGAAGATGGTTATGCTCGGCTTAAACGCTGCATCCTGATTGGTGATCATCACCAGTTGCCTCCTGTTGTTAAGAATATAGCATTCCAAAAATACAGCCACATGGATCAGAGTCTCTTTACTAGGTTTGTCCGATTGGGCATTCCTTATATTGAACTTAATGCCCAGGGTAGAGCCAGGCCAAGTATAGCCAAACTGTACAACTGGAGATACAGAGATCTGGGTGACCTTCCATATGTAAAGGAGGGTGCCATATTTCATAGAGCAAATTCTGGATTTTCCTATGAATACCAGTTGGTGGATGTGCCAGATTACAATGGCAGAGGTGAGTCTACACCTTCACCTTATTTCTTCCAAAATGTGGGGGAAGCAGAATACATTGTCAGTGTCTATATTTATATGCGTCTACTTGGGTACCCGGCAAGCAAAATCTCCATATTGACCACTTACAATGGCCAGAAGCTTTTAATCCGCGATGTTATCAACAGACGTTGTGCTCCATATGACTTCATAGGCCCTCCCAGCAAGGTTACAACAGTTGATAAATTTCAAGGTCAGCAAAATGATTTTATACTGCTCTCTCTTGTGCGTACTCGCATTGTGGGTCACCTTCGTGATGTTAGGAGATTGATTGTTGCAATGTCTCGTGCCCGACTGGGTCTTTACGTGTTTTGCCGTCGTTCTTTGTTTGAACAATGCTATGAACTTCAGCCTACATTTCAACGTCTACTTCAGAGACCTGATCACCTTGCCCTTAATTTGAATGAGATTACATCAAACACTGAGCGTCACGTTGAAGAAACTGGTCCTATTCATCTTGTCAGCAGTGTTGATGAGATGATTGGTATCTACCAGCAGCTTTATGAGGTAAAGTTTCATCAGTACATGCCTTATTCAGGTCAGGTAGGTGAACTCCTTCCGATTGATACAGATGTGCACCATCCCATGGATACAGATATGCCTGTAACTTCCGAAGGGGCACCAGACGATAATGCCCAGCATGGAAGCAATATGGAAGAAGACACTAAAGGTGGTGTCGTTGGCAATGGGCAGAACACAGAATCATTATTCAAGAACCATTCAAATGGGGAGACAGATGCTAAAGCTAGCGTGCCACCCGAAAGCAATTCTGATGAAACAGAATTGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

1524

Amino Acids

174.81

Weight (kDa)

5.68

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 48 - 460 6e-136 Intron-binding protein aquarius N-terminal
Aquarius_N_2nd PF21143 541 - 711 1.7e-55 Intron-binding protein aquarius, beta-barrel
Aquarius_N_3rd PF21144 761 - 856 5.3e-23 Intron-binding protein aquarius insert domain
AAA_11 PF13086 877 - 1164 2.8e-28 AAA domain
AAA_12 PF13087 1174 - 1366 1.4e-25 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 244
Acc65I GGTACC 1 cut(s) 3831
AccB1I GGYRCC 3 cut(s) 3665, 3831, 4396
AccB7I CCANNNNNTGG 4 cut(s) 1715, 3715, 3778, 4363
AccBSI CCGCTC 1 cut(s) 64
AccI GTMKAC 5 cut(s) 1047, 3233, 3749, 3823, 4144
AccII CGCG 4 cut(s) 356, 723, 1140, 3891
AccIII TCCGGA 1 cut(s) 2206
AclWI GGATC 4 cut(s) 818, 1310, 2283, 3531
AcoI YGGCCR 2 cut(s) 332, 3871
AcsI RAATTY 9 cut(s) 337, 908, 1276, 1540, 2588, 2691, 3375, 3685, 3962
AcuI CTGAAG 7 cut(s) 1316, 1892, 2845, 3200, 3248, 4109, 4133
AcyI GRCGYC 1 cut(s) 183
AflIII ACRYGT 3 cut(s) 1138, 2969, 4083
AjnI CCWGG 8 cut(s) 1261, 2452, 2545, 2632, 2659, 2754, 3582, 3593
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AleI CACNNNNGTG 2 cut(s) 999, 4029
Alw21I GWGCWC 5 cut(s) 369, 1006, 3373, 3916, 4356
Alw26I GTCTC 7 cut(s) 1386, 1562, 2727, 3536, 4065, 4147, 4502
Alw44I GTGCAC 1 cut(s) 4352
AlwI GGATC 4 cut(s) 818, 1310, 2283, 3531
AlwNI CAGNNNCTG 1 cut(s) 1736
Ama87I CYCGRG 1 cut(s) 65
Aor13HI TCCGGA 1 cut(s) 2206
ApaLI GTGCAC 1 cut(s) 4352
ApoI RAATTY 9 cut(s) 337, 908, 1276, 1540, 2588, 2691, 3375, 3685, 3962
ArsI GACNNNNNNTTYG 2 cut(s) 1232, 1264
AseI ATTAAT 3 cut(s) 543, 1080, 1623
Asp700I GAANNNNTTC 4 cut(s) 203, 1599, 2337, 4332
Asp718I GGTACC 1 cut(s) 3831
AspLEI GCGC 5 cut(s) 42, 100, 1227, 2093, 2901
AspS9I GGNCC 9 cut(s) 23, 136, 168, 270, 1486, 2549, 2654, 3932, 4223
AsuC2I CCSGG 3 cut(s) 74, 90, 3836
AsuII TTCGAA 3 cut(s) 207, 1368, 2407
AsuNHI GCTAGC 2 cut(s) 2842, 4523
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 5 cut(s) 136, 168, 2549, 2654, 4223
AxyI CCTNAGG 2 cut(s) 590, 2952
BaeGI GKGCMC 4 cut(s) 1893, 4069, 4356, 4399
BaeI ACNNNNGTAYC 6 cut(s) 2670, 2703, 3815, 3848, 4335, 4368
BalI TGGCCA 2 cut(s) 334, 3873
BanI GGYRCC 3 cut(s) 3665, 3831, 4396
BauI CACGAG 2 cut(s) 1016, 4062
BbsI GAAGAC 4 cut(s) 524, 1898, 3188, 4443
Bbv12I GWGCWC 5 cut(s) 369, 1006, 3373, 3916, 4356
BccI CCATC 6 cut(s) 637, 1657, 1868, 2366, 3419, 4365
BceAI ACGGC 4 cut(s) 10, 257, 2859, 4077
BciT130I CCWGG 8 cut(s) 1263, 2454, 2547, 2634, 2661, 2756, 3584, 3595
BciVI GTATCC 2 cut(s) 2565, 4360
BclI TGATCA 5 cut(s) 669, 1084, 2347, 3463, 4159
BcnI CCSGG 3 cut(s) 74, 90, 3836
BcoDI GTCTC 7 cut(s) 1386, 1562, 2727, 3536, 4065, 4147, 4502
BfaI CTAG 9 cut(s) 105, 179, 218, 828, 1767, 2843, 2934, 3540, 4524
BfmI CTRYAG 2 cut(s) 978, 1055
BfoI RGCGCY 3 cut(s) 43, 101, 2902
BfuI GTATCC 2 cut(s) 2565, 4360
BglI GCCNNNNNGGC 2 cut(s) 1897, 2871
BglII AGATCT 2 cut(s) 2179, 3634
BmcAI AGTACT 1 cut(s) 314
Bme18I GGWCC 5 cut(s) 136, 168, 2549, 2654, 4223
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 9 cut(s) 23, 136, 168, 270, 1486, 2549, 2654, 3932, 4223
BmiI GGNNCC 6 cut(s) 137, 1176, 2655, 3667, 3833, 4398
BmrI ACTGGG 1 cut(s) 4082
BmsI GCATC 7 cut(s) 847, 865, 1467, 1615, 2305, 3459, 4504
BmtI GCTAGC 2 cut(s) 2846, 4527
BmuI ACTGGG 1 cut(s) 4082
BoxI GACNNNNGTC 1 cut(s) 2547
BpiI GAAGAC 4 cut(s) 524, 1898, 3188, 4443
BplI GAGNNNNNCTC 2 cut(s) 3083, 3115
BpmI CTGGAG 5 cut(s) 320, 1491, 2927, 3395, 3643
Bpu14I TTCGAA 3 cut(s) 207, 1368, 2407
BpuEI CTTGAG 7 cut(s) 488, 787, 812, 946, 973, 1018, 3347
BpuMI CCSGG 3 cut(s) 74, 90, 3836
BsaAI YACGTR 1 cut(s) 4084
BsaBI GATNNNNATC 1 cut(s) 4262
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 4147
BsaWI WCCGGW 3 cut(s) 211, 441, 2206
Bse118I RCCGGY 1 cut(s) 138
Bse21I CCTNAGG 2 cut(s) 590, 2952
Bse3DI GCAATG 4 cut(s) 1234, 2076, 4062, 4468
Bse8I GATNNNNATC 1 cut(s) 4262
BseAI TCCGGA 1 cut(s) 2206
BseBI CCWGG 8 cut(s) 1263, 2454, 2547, 2634, 2661, 2756, 3584, 3595
BseJI GATNNNNATC 1 cut(s) 4262
BseMI GCAATG 4 cut(s) 1234, 2076, 4062, 4468
BseMII CTCAG 8 cut(s) 504, 1549, 2012, 2615, 2634, 2738, 2943, 4191
BseRI GAGGAG 2 cut(s) 1523, 2143
BseSI GKGCMC 4 cut(s) 1893, 4069, 4356, 4399
BseYI CCCAGC 3 cut(s) 2981, 3938, 4413
BsgI GTGCAG 3 cut(s) 1884, 2238, 2787
Bsh1236I CGCG 4 cut(s) 356, 723, 1140, 3891
Bsh1285I CGRYCG 2 cut(s) 78, 634
BshNI GGYRCC 3 cut(s) 3665, 3831, 4396
BsiEI CGRYCG 2 cut(s) 78, 634
BsiHKAI GWGCWC 5 cut(s) 369, 1006, 3373, 3916, 4356
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 8 cut(s) 74, 90, 139, 212, 442, 2207, 2273, 3836
BsiWI CGTACG 2 cut(s) 27, 627
BslFI GGGAC 5 cut(s) 149, 260, 1108, 2122, 2465
BsmAI GTCTC 7 cut(s) 1386, 1562, 2727, 3536, 4065, 4147, 4502
BsmBI CGTCTC 2 cut(s) 1562, 2727
BsmFI GGGAC 5 cut(s) 149, 260, 1108, 2122, 2465
BsmI GAATGC 3 cut(s) 1804, 3500, 3558
Bso31I GGTCTC 1 cut(s) 4147
BsoBI CYCGRG 1 cut(s) 65
Bsp119I TTCGAA 3 cut(s) 207, 1368, 2407
Bsp1286I GDGCHC 8 cut(s) 369, 1006, 1893, 3373, 3916, 4069, 4356, 4399
Bsp13I TCCGGA 1 cut(s) 2206
Bsp1407I TGTACA 1 cut(s) 3615
Bsp19I CCATGG 3 cut(s) 1319, 2484, 4362
BspCNI CTCAG 8 cut(s) 505, 1548, 2013, 2614, 2633, 2737, 2944, 4192
BspEI TCCGGA 1 cut(s) 2206
BspFNI CGCG 4 cut(s) 356, 723, 1140, 3891
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 6 cut(s) 137, 1176, 2655, 3667, 3833, 4398
BspMAI CTGCAG 1 cut(s) 1059
BspOI GCTAGC 2 cut(s) 2846, 4527
BspPI GGATC 4 cut(s) 818, 1310, 2283, 3531
BspQI GCTCTTC 1 cut(s) 2454
BspT104I TTCGAA 3 cut(s) 207, 1368, 2407
BspT107I GGYRCC 3 cut(s) 3665, 3831, 4396
BspTNI GGTCTC 1 cut(s) 4147
BsrBI CCGCTC 1 cut(s) 64
BsrDI GCAATG 4 cut(s) 1234, 2076, 4062, 4468
BsrFI RCCGGY 1 cut(s) 138
BsrGI TGTACA 1 cut(s) 3615
BssAI RCCGGY 1 cut(s) 138
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 2 cut(s) 1016, 4062
BssT1I CCWWGG 8 cut(s) 807, 1319, 2139, 2393, 2484, 2570, 3159, 4362
Bst2BI CACGAG 2 cut(s) 1016, 4062
Bst2UI CCWGG 8 cut(s) 1263, 2454, 2547, 2634, 2661, 2756, 3584, 3595
Bst6I CTCTTC 8 cut(s) 249, 490, 777, 1863, 1944, 2454, 3198, 4560
BstACI GRCGYC 1 cut(s) 183
BstAUI TGTACA 1 cut(s) 3615
BstBAI YACGTR 1 cut(s) 4084
BstBI TTCGAA 3 cut(s) 207, 1368, 2407
BstDSI CCRYGG 3 cut(s) 1319, 2484, 4362
BstEII GGTNACC 2 cut(s) 3641, 4022
BstENI CCTNNNNNAGG 4 cut(s) 2010, 2544, 2664, 4311
BstFNI CGCG 4 cut(s) 356, 723, 1140, 3891
BstH2I RGCGCY 3 cut(s) 43, 101, 2902
BstHHI GCGC 5 cut(s) 42, 100, 1227, 2093, 2901
BstMAI GTCTC 7 cut(s) 1386, 1562, 2727, 3536, 4065, 4147, 4502
BstMCI CGRYCG 2 cut(s) 78, 634
BstNI CCWGG 8 cut(s) 1263, 2454, 2547, 2634, 2661, 2756, 3584, 3595
BstNSI RCATGY 4 cut(s) 1616, 2973, 3294, 4306
BstPAI GACNNNNGTC 1 cut(s) 2547
BstPI GGTNACC 2 cut(s) 3641, 4022
BstSFI CTRYAG 2 cut(s) 978, 1055
BstSLI GKGCMC 4 cut(s) 1893, 4069, 4356, 4399
BstUI CGCG 4 cut(s) 356, 723, 1140, 3891
BstV2I GAAGAC 4 cut(s) 524, 1898, 3188, 4443
BstX2I RGATCY 3 cut(s) 2179, 2275, 3634
BstXI CCANNNNNNTGG 2 cut(s) 1901, 4502
BstYI RGATCY 3 cut(s) 2179, 2275, 3634
Bsu36I CCTNAGG 2 cut(s) 590, 2952
BsuI GTATCC 2 cut(s) 2565, 4360
BtgI CCRYGG 3 cut(s) 1319, 2484, 4362
BtgZI GCGATG 1 cut(s) 3906
BtsI GCAGTG 2 cut(s) 1674, 4249
BtsIMutI CAGTG 6 cut(s) 1409, 1674, 1699, 3808, 4197, 4249
CaiI CAGNNNCTG 1 cut(s) 1736
CciI TCATGA 1 cut(s) 537
CfoI GCGC 5 cut(s) 42, 100, 1227, 2093, 2901
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 9 cut(s) 23, 136, 168, 270, 1486, 2549, 2654, 3932, 4223
CseI GACGC 4 cut(s) 191, 719, 3809, 4193
CsiI ACCWGGT 1 cut(s) 2659
CspCI CAANNNNNGTGG 2 cut(s) 2631, 2666
DraI TTTAAA 3 cut(s) 1168, 2155, 2299
DrdI GACNNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 2 cut(s) 332, 3871
Eam1104I CTCTTC 8 cut(s) 249, 490, 777, 1863, 1944, 2454, 3198, 4560
EarI CTCTTC 8 cut(s) 249, 490, 777, 1863, 1944, 2454, 3198, 4560
Eco130I CCWWGG 8 cut(s) 807, 1319, 2139, 2393, 2484, 2570, 3159, 4362
Eco147I AGGCCT 1 cut(s) 2398
Eco31I GGTCTC 1 cut(s) 4147
Eco47I GGWCC 5 cut(s) 136, 168, 2549, 2654, 4223
Eco57I CTGAAG 7 cut(s) 1316, 1892, 2845, 3200, 3248, 4109, 4133
Eco81I CCTNAGG 2 cut(s) 590, 2952
Eco88I CYCGRG 1 cut(s) 65
Eco91I GGTNACC 2 cut(s) 3641, 4022
EcoNI CCTNNNNNAGG 4 cut(s) 2010, 2544, 2664, 4311
EcoO109I RGGNCCY 1 cut(s) 3932
EcoO65I GGTNACC 2 cut(s) 3641, 4022
EcoRII CCWGG 8 cut(s) 1261, 2452, 2545, 2632, 2659, 2754, 3582, 3593
EcoT14I CCWWGG 8 cut(s) 807, 1319, 2139, 2393, 2484, 2570, 3159, 4362
ErhI CCWWGG 8 cut(s) 807, 1319, 2139, 2393, 2484, 2570, 3159, 4362
Esp3I CGTCTC 2 cut(s) 1562, 2727
FaqI GGGAC 5 cut(s) 149, 260, 1108, 2122, 2465
FauI CCCGC 3 cut(s) 69, 126, 3144
FauNDI CATATG 3 cut(s) 1570, 3652, 3919
FbaI TGATCA 5 cut(s) 669, 1084, 2347, 3463, 4159
FblI GTMKAC 5 cut(s) 1047, 3233, 3749, 3823, 4144
FspBI CTAG 9 cut(s) 105, 179, 218, 828, 1767, 2843, 2934, 3540, 4524
GlaI GCGC 5 cut(s) 41, 99, 1226, 2092, 2900
GsaI CCCAGC 3 cut(s) 2985, 3942, 4417
GsuI CTGGAG 5 cut(s) 320, 1491, 2927, 3395, 3643
HaeII RGCGCY 3 cut(s) 43, 101, 2902
HapII CCGG 8 cut(s) 74, 90, 139, 212, 442, 2207, 2273, 3836
HgaI GACGC 4 cut(s) 191, 719, 3809, 4193
HhaI GCGC 5 cut(s) 42, 100, 1227, 2093, 2901
Hin1I GRCGYC 1 cut(s) 183
Hin6I GCGC 5 cut(s) 40, 98, 1225, 2091, 2899
HinP1I GCGC 5 cut(s) 40, 98, 1225, 2091, 2899
HincII GTYRAC 1 cut(s) 1048
HindII GTYRAC 1 cut(s) 1048
HindIII AAGCTT 1 cut(s) 3878
HpaII CCGG 8 cut(s) 74, 90, 139, 212, 442, 2207, 2273, 3836
Hpy166II GTNNAC 9 cut(s) 1048, 2657, 3007, 3234, 3750, 3824, 4145, 4328, 4354
Hpy8I GTNNAC 9 cut(s) 1048, 2657, 3007, 3234, 3750, 3824, 4145, 4328, 4354
Hpy99I CGWCG 1 cut(s) 4098
HpyCH4IV ACGT 6 cut(s) 369, 3059, 3907, 4083, 4141, 4209
HpySE526I ACGT 6 cut(s) 369, 3059, 3907, 4083, 4141, 4209
Hsp92I GRCGYC 1 cut(s) 183
HspAI GCGC 5 cut(s) 40, 98, 1225, 2091, 2899
Kpn2I TCCGGA 1 cut(s) 2206
KpnI GGTACC 1 cut(s) 3835
Ksp22I TGATCA 5 cut(s) 669, 1084, 2347, 3463, 4159
LguI GCTCTTC 1 cut(s) 2454
LmnI GCTCC 5 cut(s) 214, 2609, 3019, 3414, 3919
LweI GCATC 7 cut(s) 847, 865, 1467, 1615, 2305, 3459, 4504
MabI ACCWGGT 1 cut(s) 2659
MaeI CTAG 9 cut(s) 105, 179, 218, 828, 1767, 2843, 2934, 3540, 4524
MaeII ACGT 6 cut(s) 369, 3059, 3907, 4083, 4141, 4209
MbiI CCGCTC 1 cut(s) 64
MflI RGATCY 3 cut(s) 2179, 2275, 3634
MhlI GDGCHC 8 cut(s) 369, 1006, 1893, 3373, 3916, 4069, 4356, 4399
MlsI TGGCCA 2 cut(s) 334, 3873
MluI ACGCGT 1 cut(s) 1138
MluNI TGGCCA 2 cut(s) 334, 3873
MlyI GAGTC 3 cut(s) 3140, 3538, 3755
MmeI TCCRAC 3 cut(s) 102, 2587, 3126
Mox20I TGGCCA 2 cut(s) 334, 3873
MroI TCCGGA 1 cut(s) 2206
MroXI GAANNNNTTC 4 cut(s) 203, 1599, 2337, 4332
MscI TGGCCA 2 cut(s) 334, 3873
MslI CAYNNNNRTG 7 cut(s) 999, 1611, 2064, 2891, 3401, 4029, 4500
Msp20I TGGCCA 2 cut(s) 334, 3873
MspA1I CMGCKG 3 cut(s) 133, 1733, 2684
MspI CCGG 8 cut(s) 74, 90, 139, 212, 442, 2207, 2273, 3836
Mva1269I GAATGC 3 cut(s) 1804, 3500, 3558
MvaI CCWGG 8 cut(s) 1263, 2454, 2547, 2634, 2661, 2756, 3584, 3595
MvnI CGCG 4 cut(s) 356, 723, 1140, 3891
NciI CCSGG 3 cut(s) 74, 90, 3836
NcoI CCATGG 3 cut(s) 1319, 2484, 4362
NdeI CATATG 3 cut(s) 1570, 3652, 3919
NheI GCTAGC 2 cut(s) 2842, 4523
NlaIV GGNNCC 6 cut(s) 137, 1176, 2655, 3667, 3833, 4398
NmeAIII GCCGAG 2 cut(s) 79, 3415
NmuCI GTSAC 7 cut(s) 279, 1265, 1738, 2029, 3641, 4022, 4205
NspI RCATGY 4 cut(s) 1616, 2973, 3294, 4306
NspV TTCGAA 3 cut(s) 207, 1368, 2407
OliI CACNNNNGTG 2 cut(s) 999, 4029
PaeI GCATGC 1 cut(s) 1616
PaeR7I CTCGAG 1 cut(s) 65
PagI TCATGA 1 cut(s) 537
PasI CCCWGGG 1 cut(s) 3583
PceI AGGCCT 1 cut(s) 2398
PciI ACATGT 1 cut(s) 2969
PciSI GCTCTTC 1 cut(s) 2454
PctI GAATGC 3 cut(s) 1804, 3500, 3558
PdmI GAANNNNTTC 4 cut(s) 203, 1599, 2337, 4332
Pfl23II CGTACG 2 cut(s) 27, 627
PflFI GACNNNGTC 1 cut(s) 4074
PflMI CCANNNNNTGG 4 cut(s) 1715, 3715, 3778, 4363
PleI GAGTC 3 cut(s) 3139, 3537, 3754
PpsI GAGTC 3 cut(s) 3139, 3537, 3754
Ppu21I YACGTR 1 cut(s) 4084
PscI ACATGT 1 cut(s) 2969
PshAI GACNNNNGTC 1 cut(s) 2547
PshBI ATTAAT 3 cut(s) 543, 1080, 1623
Psp6I CCWGG 8 cut(s) 1261, 2452, 2545, 2632, 2659, 2754, 3582, 3593
PspEI GGTNACC 2 cut(s) 3641, 4022
PspFI CCCAGC 3 cut(s) 2981, 3938, 4413
PspGI CCWGG 8 cut(s) 1261, 2452, 2545, 2632, 2659, 2754, 3582, 3593
PspLI CGTACG 2 cut(s) 27, 627
PspN4I GGNNCC 6 cut(s) 137, 1176, 2655, 3667, 3833, 4398
PspPI GGNCC 9 cut(s) 23, 136, 168, 270, 1486, 2549, 2654, 3932, 4223
PspXI VCTCGAGB 1 cut(s) 65
PstI CTGCAG 1 cut(s) 1059
PstNI CAGNNNCTG 1 cut(s) 1736
PsuI RGATCY 3 cut(s) 2179, 2275, 3634
PsyI GACNNNGTC 1 cut(s) 4074
PvuII CAGCTG 1 cut(s) 1733
RseI CAYNNNNRTG 7 cut(s) 999, 1611, 2064, 2891, 3401, 4029, 4500
SalI GTCGAC 1 cut(s) 1046
SapI GCTCTTC 1 cut(s) 2454
Sau96I GGNCC 9 cut(s) 23, 136, 168, 270, 1486, 2549, 2654, 3932, 4223
ScaI AGTACT 1 cut(s) 314
SchI GAGTC 3 cut(s) 3140, 3538, 3755
SduI GDGCHC 8 cut(s) 369, 1006, 1893, 3373, 3916, 4069, 4356, 4399
SexAI ACCWGGT 1 cut(s) 2659
SfaNI GCATC 7 cut(s) 847, 865, 1467, 1615, 2305, 3459, 4504
SfcI CTRYAG 2 cut(s) 978, 1055
Sfr274I CTCGAG 1 cut(s) 65
SfuI TTCGAA 3 cut(s) 207, 1368, 2407
SinI GGWCC 5 cut(s) 136, 168, 2549, 2654, 4223
SlaI CTCGAG 1 cut(s) 65
SmiMI CAYNNNNRTG 7 cut(s) 999, 1611, 2064, 2891, 3401, 4029, 4500
SmlI CTYRAG 8 cut(s) 65, 467, 766, 791, 925, 952, 997, 3326
SmoI CTYRAG 8 cut(s) 65, 467, 766, 791, 925, 952, 997, 3326
SphI GCATGC 1 cut(s) 1616
SseBI AGGCCT 1 cut(s) 2398
SspI AATATT 2 cut(s) 1714, 2195
SspMI CTAG 9 cut(s) 105, 179, 218, 828, 1767, 2843, 2934, 3540, 4524
StuI AGGCCT 1 cut(s) 2398
StyI CCWWGG 8 cut(s) 807, 1319, 2139, 2393, 2484, 2570, 3159, 4362
TaiI ACGT 6 cut(s) 372, 3062, 3910, 4086, 4144, 4212
TatI WGTACW 4 cut(s) 312, 888, 3615, 4299
TauI GCSGC 2 cut(s) 158, 2899
TscAI CASTG 6 cut(s) 1416, 1674, 1699, 3808, 4204, 4249
TseFI GTSAC 7 cut(s) 279, 1265, 1738, 2029, 3641, 4022, 4205
Tsp45I GTSAC 7 cut(s) 279, 1265, 1738, 2029, 3641, 4022, 4205
TspGWI ACGGA 3 cut(s) 254, 2541, 2709
TspRI CASTG 6 cut(s) 1416, 1674, 1699, 3808, 4204, 4249
Tth111I GACNNNGTC 1 cut(s) 4074
Van91I CCANNNNNTGG 4 cut(s) 1715, 3715, 3778, 4363
VneI GTGCAC 1 cut(s) 4352
VpaK11BI GGWCC 5 cut(s) 136, 168, 2549, 2654, 4223
VspI ATTAAT 3 cut(s) 543, 1080, 1623
XagI CCTNNNNNAGG 4 cut(s) 2010, 2544, 2664, 4311
XapI RAATTY 9 cut(s) 337, 908, 1276, 1540, 2588, 2691, 3375, 3685, 3962
XceI RCATGY 4 cut(s) 1616, 2973, 3294, 4306
XhoI CTCGAG 1 cut(s) 65
XmiI GTMKAC 5 cut(s) 1047, 3233, 3749, 3823, 4144
XmnI GAANNNNTTC 4 cut(s) 203, 1599, 2337, 4332
XspI CTAG 9 cut(s) 105, 179, 218, 828, 1767, 2843, 2934, 3540, 4524
ZrmI AGTACT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.