Rh1DG027000

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0008765
Physical Location & Seq
Forward (+)
4101052 .. 4116147
15096 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG027000.1

Sequence Viewer

Length: 4347 bp
ATGACCAAGGTGTACGGAACAGGCGCGTACGATTTCAAGCGCCACCATGTTGCAGAGTACCCGGTGGAGCTGAACCACCAGCTCGGAGACAAGCCGGTGGTGGAGGCTAAACCCGGTGCGGCGCTGCCGAGCTCGATAACGCTGTCGGAGATTCAGAGGGACCAGCTCACTATGATTGCCGCTGCCAATTGGTCCAAAGCCGGCGGCGACGCCGCGCAGGTGAAGAAGGCGTTCGACGCGGAGCTGGTGAAGAAGATTTACGAGACGGAGTTGTGTGTGAAGGAGGGGCAGAGGAAGACGGTGCCATTGCAGAGAGTGATGATTTTGGAGGTCAGCCAGTATTTGGAGAACTATCTCTTTCCCAATTTCGATCCCGATACGGCGACGTTCGAGCACGTTATGTCCATGATTCTCATGGTCAATGAGAAGTTTCGAGAGAATGTGGCAGCTTGGGTATGCTTTCATGACTGGAAAGATGCGTTTAAAGGATTCCTTGGAAGGGTTCTTCGACTTAAATCGGAAAGAGAATTAAGTATAGCAGAGAAGACAAATTATCTAGTATTCATGATAAATGCATTTCAGAGTTTGGAAGATGAGATAGTCAGTGAGACTGTCCTTAGGTTGGCAAGTTTCCAATCCTGGCACAGTTTGTCGTATGGTCGTTTTCAGATGGAGCTAGGTTTAAATTCAGATTTGATCAAGAAATGGAGAAGGATGATAAAGAGGGAAGCTGCAAAACATGGAGAGTCTTTTAACCCCTCAACTACACTTGAAGTCCAGTTTTTGAGAAACCTCATCGAAGAATTCCTGGAGATACTTGATTCCAAAGTCTTACCGCCAAATCATGGTGTCAATGGAGAGGATCAACTTCTTGCTGCCAATGGAATGGAACACATTGATGATGCTTGTTTGCAAGAGCTTGCATTGGCCAACATTGGTTCAATTGACAACCGAAATGATCTCACTAAGAGATTGTCTGTTCTTTCTCATGAAGAATTGAAGGATTTGGTTTGTTCAAAGTTGAAACTGATTTCTAAGAATGATCCTTGGTCATCCAGGGTTGATTTCCTTACTGAAGTGATGGTTTCCTTTTTCAAGAGGCAACAGTCTCAAAAAGAAAAAATAAATGCTCTTCCACTGTATCCAAATGAGCTTATCATGTGGGATGAAAGCCTTGTGCCAAGCATTAATTACTCTGGAGAAGGTTGTCTGGCTCTTCCAAAACTTAATTTACAATTTTTGACGCTGCATGATTATCTCCTGAGAAATTTTGATCTCTTCCGCCTTGAATCAACATATGAGATCCGTGAGGATATTCAGGAGGCTGTGCCACATCTGAATGCCTGCATTAATAATGAGGGAGAAACTGCTTTCCGTGGTTGGTCAAGGATGGCTGTGCCGATTAAAGAATTCAAGATCAGTGAGGTAAAACAGCCAAATATTGGAGAAGTTAAACCAGCTGCTGTGACAGCAGAAATCACTTTTAGTATATCCAGTTATAGAGCACAGGTACGATCTGAATGGAATGCTCTTAAAGAGCATGATGTTCTATTTTTACTTTCCATACGCCCTTCCTTTGAGCCTCTAAGTGCAGAAGAGGGTGCCAAGGCTAGTGTGCCTCAGAGGCTTGGTCTTCAATATGTACGGGGTTGTGAAATCATTGAGGTTCGTGATGAGGAGGGAACTCTAATGAATGATTTCACTGGAAGAATTAAACGGGATGAGTGGAAGCCTCCAAAAGGAGAGTTGAGAACTTTGACTGTTGCTTTGGATACCGCTCAGTACTACATGGATGTTAGCAACACTGCTGCAAAGGGTGCAGAAGATGTTTATGGGACATTCAATATTTTGATGAGGAGAAAGCCCAAGGAAAATAATTTCAAAGCAATTTTAGAATCCATTAGAGATCTTATGAATGAATATTGTATTGTTCCTGACTGGTTGCACAAAACATTTCTGGGGTATGGTAATCCTTCTGCCGCACAGTGGACTAATATGCCAGATCTCTTAGAAACTGTAGATTTCAAAGATACTTTCCTTGATGCAGACCATCTGAAGGAATGTTTTCCAGATGATCAGGTTTTCTTTGTTAGTCCAGATGGTATAGAAAATATGGATCCAAGGCCTCCTTTTCGAATAAAGCTCCCCAAGAAGATCAAAAGCAGCACTAATGCTCTTCCGGGCAATAAGAAAGCAAAGATTAATTCTATGAGTGATGCCCCCATTGAGGACTCTGATAGTGAGAAAGAAAAATTTGTTGTTGAGGCATACACCCCTCCTGACCCAGGTCCTTATCCGCAAGACCAGCCAAGGCAGAACTCAGTTAGATTTACACCCACTCAGGTTGGAGCAATCCTATCAGGTATTCAGCCAGGACTAACAATGGTTGTGGGTCCACCTGGTACGGGAAAGACTGATACAGCCGTGCAAATTTTGAATGTTCTGTATCATAATTGTCCATCTCAGAGAACATTGATAATTACTCACTCAAACCAGGCTCTAAATGACCTTTTTGAAAAGATAATGCAGAGGGATGTACCTGCACGCTATCTTCTTCGACTTGGGCAAGGAGAACAAGAGCTAGCAACTGATCTTGACTTCAGTCGGCAAGGCCGTGTCAATGCCATGCTTGTGAGGCGGTTGGAGTTGCTTAGTGAAGTGGAGAGGCTGGCAAGATCTCTCCAATTGCCCGAGGATGTTGGTTATACATGTGAAACTGCTGGATACTTTTGGTTGCTTCATGTATACTCGCGCTGGGAGCAATTCCTGGCTGCTTGTAACCAAAATAAAAATAAACAATCATTTGTTAAAGATCGATTTCCCTTCAAGGAATTCTTCTCCAACGCACCAAAGCCAGTTTTTACTGGTGAGTCATTTGAGAAAGATATGCGGGCAGCTAAGGGTTGCTTTCGTCATCTAAAGACTGTGTTTCAAGAGCTTGAAGAGTGTAGGGCTTTTGAGTTACTGAAGTCTACAGCTGATCGAGCAAACTACTTGATGACGAAGCAGGCAAAGATAGTTGCCATGACATGCACTCACGCAGCTCTGAAGAGGAAGGATTTTCTCCAGTTAGGTTTCAAGTACGATAACTTGCTGATGGAAGAAAGTGCTCAAATTTTGGAGATCGAAACTTTCATTCCAATGTTGCTCCAGAGGCAGGAAGATGGTTATGCACGGCTTAAACGTTGTATCCTGATTGGTGATCATCACCAGTTGCCTCCTGTTGTCAAGAATATGGCCTTTCAAAAGTATAGCCACATGGACCAGAGTCTCTTTACAAGGTTCGTCCGTTTGGGCATTCCTTATATTGAACTTAATGCTCAGGGTAGGGCCAGGCCAAGTATAGCCAAACTTTACAACTGGAGGTATAGAGAACTAGGAGACCTTCCTTATGTAAAGGAAGATGCTGTTTACAATAGAGCAAATTCTGGATTCTCCTTTGAATATCAATTGGTAGATGTACCGGATTACCATGATAGAGGTGAGTCTGCTCCATCACCTTGGTTCTACCAAAATGAGGGAGAAGCTGAATATATCGTCAGTGTGTATATCTACATGCGTCTACTTGGGTACCCTGCAAACAAGATATCAATATTGACCACTTACAATGGCCAGAAGCTATTAATCCGTGATGTTATCAACAGACGATGTGCTCCGTATGACTTCATAGGCCCTCCCAGTAAGGTTACAACAGTTGACAAATTTCAGGGTCAGCAAAATGATTTTATATTGCTCTCTCTTGTGCGTACTCGCTTTGTGGGTCACCTTCGTGATGTTAGGAGATTGATTGTTGCCATGTCTCGTGCCCGATTGGGTCTGTATGTGTTTTGCCGCCGTTCTTTGTTTGAGCAATGCTACGAACTTCAGCCTACCTTCCAACTTTTACTTCAGAGACCTGATTGCCTTGCTCTGAATTTTAATGAGACTACACCACACACAGAACGCCATGTTGAAGAAACGGGGCCTGTCCATCTTGTAAGCAGTGTTGATGAGATGATCAGTATCTACCAGCAGCTTTATGCGGTGAAGTTTCATCAGTATGTTGCTCCTTCAAGTCAATTAGTTCCATCCATGGACGCATTGGGAGAGCAAACAAGTCAGCAAACTGATATGTCTGGGCACATTCCTATGGATGCAGATATCCCTGTATCTGCTGATGGGGCGCCAGACGATACACTGCATGGAAACAATTCAGAACTGGGCGAAGAATTGGATGTCGCAAATGGCCAAAAAGTGGATTCGTCGATTGAGAATCATACAAATGGGGTGATGGAAATGGAAACTAGTCTGGATGATGCACATGTGGCTCCTGAAAGCAATTCGGATCAAGTGAAAATGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

1448

Amino Acids

165.32

Weight (kDa)

5.58

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 56 - 276 1.3e-63 Intron-binding protein aquarius N-terminal
Aquarius_N_1st PF16399 303 - 369 1.2e-13 Intron-binding protein aquarius N-terminal
Aquarius_N_2nd PF21143 451 - 620 6.8e-57 Intron-binding protein aquarius, beta-barrel
Aquarius_N_3rd PF21144 670 - 769 6e-21 Intron-binding protein aquarius insert domain
AAA_11 PF13086 782 - 1078 1.1e-28 AAA domain
AAA_12 PF13087 1087 - 1279 6e-27 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 208
Acc36I ACCTGC 2 cut(s) 208, 2548
Acc65I GGTACC 1 cut(s) 3570
AccB1I GGYRCC 4 cut(s) 301, 1601, 3570, 4162
AccB7I CCANNNNNTGG 4 cut(s) 343, 845, 1442, 4234
AccBSI CCGCTC 1 cut(s) 1778
AccI GTMKAC 3 cut(s) 2745, 2972, 3562
AccII CGCG 4 cut(s) 26, 215, 239, 2752
AclI AACGTT 1 cut(s) 3184
AclWI GGATC 7 cut(s) 365, 870, 1037, 1297, 2111, 2124, 4332
AcoI YGGCCR 3 cut(s) 927, 3610, 4225
AcuI CTGAAG 7 cut(s) 1095, 2075, 2584, 2987, 3068, 3848, 3872
AcyI GRCGYC 2 cut(s) 210, 4163
AflIII ACRYGT 2 cut(s) 2708, 4300
AhlI ACTAGT 1 cut(s) 4283
AjnI CCWGG 9 cut(s) 638, 807, 1055, 2284, 2371, 2398, 2493, 2766, 3332
AjuI GAANNNNNNNTTGG 2 cut(s) 3870, 3902
AleI CACNNNNGTG 1 cut(s) 3768
Alw21I GWGCWC 5 cut(s) 134, 396, 1507, 3112, 3655
Alw26I GTCTC 9 cut(s) 81, 257, 602, 1113, 3275, 3375, 3804, 3886, 3917
AlwI GGATC 7 cut(s) 365, 870, 1037, 1297, 2111, 2124, 4332
AlwNI CAGNNNCTG 1 cut(s) 1463
Ama87I CYCGRG 1 cut(s) 2690
ArsI GACNNNNNNTTYG 2 cut(s) 1750, 1782
AseI ATTAAT 4 cut(s) 1188, 1350, 2202, 3623
Asp700I GAANNNNTTC 4 cut(s) 230, 1697, 2064, 4189
Asp718I GGTACC 1 cut(s) 3570
AspLEI GCGC 6 cut(s) 26, 42, 124, 217, 2754, 4165
AspS9I GGNCC 8 cut(s) 160, 192, 2288, 2393, 3262, 3330, 3671, 3962
AsuC2I CCSGG 3 cut(s) 62, 114, 2181
AsuII TTCGAA 1 cut(s) 2134
AsuNHI GCTAGC 1 cut(s) 2581
AvaI CYCGRG 1 cut(s) 2690
AvaII GGWCC 5 cut(s) 160, 192, 2288, 2393, 3262
AxyI CCTNAGG 1 cut(s) 617
BaeGI GKGCMC 2 cut(s) 3808, 4122
BaeI ACNNNNGTAYC 4 cut(s) 2409, 2442, 3554, 3587
BalI TGGCCA 3 cut(s) 929, 3612, 4227
BamHI GGATCC 1 cut(s) 2116
BanI GGYRCC 4 cut(s) 301, 1601, 3570, 4162
BanII GRGCYC 1 cut(s) 134
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BauI CACGAG 1 cut(s) 3801
BbsI GAAGAC 3 cut(s) 302, 551, 1625
Bbv12I GWGCWC 5 cut(s) 134, 396, 1507, 3112, 3655
BceAI ACGGC 5 cut(s) 396, 2408, 2598, 3191, 3819
BciT130I CCWGG 9 cut(s) 640, 809, 1057, 2286, 2373, 2400, 2495, 2768, 3334
BciVI GTATCC 4 cut(s) 1152, 1765, 2717, 3200
BclI TGATCA 4 cut(s) 696, 2074, 3202, 3996
BcnI CCSGG 3 cut(s) 62, 114, 2181
BcoDI GTCTC 9 cut(s) 81, 257, 602, 1113, 3275, 3375, 3804, 3886, 3917
BcuI ACTAGT 1 cut(s) 4283
BfaI CTAG 6 cut(s) 557, 677, 1611, 2582, 3377, 4284
BfmI CTRYAG 2 cut(s) 2016, 2973
BfoI RGCGCY 3 cut(s) 43, 125, 4166
BfuAI ACCTGC 2 cut(s) 208, 2548
BfuI GTATCC 4 cut(s) 1152, 1765, 2717, 3200
BglI GCCNNNNNGGC 1 cut(s) 1624
BglII AGATCT 3 cut(s) 1906, 2002, 2675
BmcAI AGTACT 1 cut(s) 1784
Bme18I GGWCC 5 cut(s) 160, 192, 2288, 2393, 3262
BmeT110I CYCGRG 1 cut(s) 2690
BmgT120I GGNCC 8 cut(s) 160, 192, 2288, 2393, 3262, 3330, 3671, 3962
BmiI GGNNCC 9 cut(s) 161, 303, 1603, 2118, 2394, 3572, 3963, 4164, 4308
BmrI ACTGGG 2 cut(s) 3672, 4208
BmsI GCATC 7 cut(s) 466, 892, 2032, 2206, 3394, 4123, 4285
BmtI GCTAGC 1 cut(s) 2585
BmuI ACTGGG 2 cut(s) 3672, 4208
BoxI GACNNNNGTC 3 cut(s) 2286, 2601, 3267
BpiI GAAGAC 3 cut(s) 302, 551, 1625
BpmI CTGGAG 5 cut(s) 830, 1218, 3050, 3134, 3382
Bpu10I CCTNAGC 2 cut(s) 2898, 3321
Bpu14I TTCGAA 1 cut(s) 2134
BpuMI CCSGG 3 cut(s) 62, 114, 2181
Bsa29I ATCGAT 1 cut(s) 2815
BsaBI GATNNNNATC 1 cut(s) 4001
BsaHI GRCGYC 2 cut(s) 210, 4163
BsaI GGTCTC 2 cut(s) 3375, 3886
BsaWI WCCGGW 1 cut(s) 3463
Bse118I RCCGGY 2 cut(s) 94, 200
Bse21I CCTNAGG 1 cut(s) 617
Bse3DI GCAATG 2 cut(s) 305, 3857
Bse8I GATNNNNATC 1 cut(s) 4001
BseBI CCWGG 9 cut(s) 640, 809, 1057, 2286, 2373, 2400, 2495, 2768, 3334
BseCI ATCGAT 1 cut(s) 2815
BseJI GATNNNNATC 1 cut(s) 4001
BseMI GCAATG 2 cut(s) 305, 3857
BseMII CTCAG 7 cut(s) 1253, 1634, 1793, 2334, 2354, 2477, 3335
BseRI GAGGAG 2 cut(s) 1691, 1870
BseSI GKGCMC 2 cut(s) 3808, 4122
BseYI CCCAGC 1 cut(s) 2754
BsgI GTGCAG 3 cut(s) 1611, 1839, 2526
Bsh1236I CGCG 4 cut(s) 26, 215, 239, 2752
BshNI GGYRCC 4 cut(s) 301, 1601, 3570, 4162
BshVI ATCGAT 1 cut(s) 2815
BsiHKAI GWGCWC 5 cut(s) 134, 396, 1507, 3112, 3655
BsiHKCI CYCGRG 1 cut(s) 2690
BsiSI CCGG 6 cut(s) 62, 95, 114, 201, 2180, 3464
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 2 cut(s) 173, 1849
BsmAI GTCTC 9 cut(s) 81, 257, 602, 1113, 3275, 3375, 3804, 3886, 3917
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 2 cut(s) 173, 1849
BsmI GAATGC 3 cut(s) 1345, 1531, 3297
Bso31I GGTCTC 2 cut(s) 3375, 3886
BsoBI CYCGRG 1 cut(s) 2690
Bsp119I TTCGAA 1 cut(s) 2134
Bsp1286I GDGCHC 7 cut(s) 134, 396, 1507, 3112, 3655, 3808, 4122
Bsp19I CCATGG 1 cut(s) 4071
BspCNI CTCAG 7 cut(s) 1254, 1633, 1792, 2333, 2353, 2476, 3334
BspDI ATCGAT 1 cut(s) 2815
BspFNI CGCG 4 cut(s) 26, 215, 239, 2752
BspHI TCATGA 3 cut(s) 463, 564, 988
BspLI GGNNCC 9 cut(s) 161, 303, 1603, 2118, 2394, 3572, 3963, 4164, 4308
BspMI ACCTGC 2 cut(s) 208, 2548
BspOI GCTAGC 1 cut(s) 2585
BspPI GGATC 7 cut(s) 365, 870, 1037, 1297, 2111, 2124, 4332
BspQI GCTCTTC 3 cut(s) 1137, 1221, 2181
BspT104I TTCGAA 1 cut(s) 2134
BspT107I GGYRCC 4 cut(s) 301, 1601, 3570, 4162
BspTNI GGTCTC 2 cut(s) 3375, 3886
BsrBI CCGCTC 1 cut(s) 1778
BsrDI GCAATG 2 cut(s) 305, 3857
BsrFI RCCGGY 2 cut(s) 94, 200
BssAI RCCGGY 2 cut(s) 94, 200
BssNAI GTATAC 1 cut(s) 2746
BssNI GRCGYC 2 cut(s) 210, 4163
BssSI CACGAG 1 cut(s) 3801
BssT1I CCWWGG 9 cut(s) 6, 493, 1046, 1605, 1866, 2120, 2309, 3500, 4071
Bst1107I GTATAC 1 cut(s) 2746
Bst2BI CACGAG 1 cut(s) 3801
Bst2UI CCWGG 9 cut(s) 640, 809, 1057, 2286, 2373, 2400, 2495, 2768, 3334
Bst6I CTCTTC 8 cut(s) 1137, 1221, 1283, 1590, 2181, 2937, 3044, 4335
BstACI GRCGYC 2 cut(s) 210, 4163
BstAPI GCANNNNNTGC 1 cut(s) 1817
BstBI TTCGAA 1 cut(s) 2134
BstC8I GCNNGC 8 cut(s) 202, 921, 1345, 2545, 2583, 2670, 2892, 3009
BstDSI CCRYGG 2 cut(s) 1375, 4071
BstEII GGTNACC 1 cut(s) 3761
BstENI CCTNNNNNAGG 2 cut(s) 1737, 2283
BstFNI CGCG 4 cut(s) 26, 215, 239, 2752
BstH2I RGCGCY 3 cut(s) 43, 125, 4166
BstHHI GCGC 6 cut(s) 26, 42, 124, 217, 2754, 4165
BstMAI GTCTC 9 cut(s) 81, 257, 602, 1113, 3275, 3375, 3804, 3886, 3917
BstNI CCWGG 9 cut(s) 640, 809, 1057, 2286, 2373, 2400, 2495, 2768, 3334
BstNSI RCATGY 4 cut(s) 2712, 3033, 3559, 4304
BstPAI GACNNNNGTC 3 cut(s) 2286, 2601, 3267
BstPI GGTNACC 1 cut(s) 3761
BstSFI CTRYAG 2 cut(s) 2016, 2973
BstSLI GKGCMC 2 cut(s) 3808, 4122
BstUI CGCG 4 cut(s) 26, 215, 239, 2752
BstV2I GAAGAC 3 cut(s) 302, 551, 1625
BstX2I RGATCY 5 cut(s) 1302, 1906, 2002, 2116, 2675
BstXI CCANNNNNNTGG 2 cut(s) 886, 3501
BstYI RGATCY 5 cut(s) 1302, 1906, 2002, 2116, 2675
BstZ17I GTATAC 1 cut(s) 2746
Bsu15I ATCGAT 1 cut(s) 2815
Bsu36I CCTNAGG 1 cut(s) 617
BsuI GTATCC 4 cut(s) 1152, 1765, 2717, 3200
BsuTUI ATCGAT 1 cut(s) 2815
BtgI CCRYGG 2 cut(s) 1375, 4071
BtsI GCAGTG 3 cut(s) 1803, 3988, 4175
BtsIMutI CAGTG 9 cut(s) 610, 1136, 1426, 1701, 1803, 1991, 3547, 3988, 4175
BveI ACCTGC 2 cut(s) 208, 2548
Cac8I GCNNGC 8 cut(s) 202, 921, 1345, 2545, 2583, 2670, 2892, 3009
CaiI CAGNNNCTG 1 cut(s) 1463
CciI TCATGA 3 cut(s) 463, 564, 988
CfoI GCGC 6 cut(s) 26, 42, 124, 217, 2754, 4165
Cfr10I RCCGGY 2 cut(s) 94, 200
Cfr13I GGNCC 8 cut(s) 160, 192, 2288, 2393, 3262, 3330, 3671, 3962
ClaI ATCGAT 1 cut(s) 2815
CseI GACGC 5 cut(s) 218, 245, 1252, 3548, 4085
CsiI ACCWGGT 1 cut(s) 2398
CspCI CAANNNNNGTGG 4 cut(s) 2326, 2361, 2370, 2405
DinI GGCGCC 1 cut(s) 4164
DraI TTTAAA 2 cut(s) 484, 684
EaeI YGGCCR 3 cut(s) 927, 3610, 4225
Eam1104I CTCTTC 8 cut(s) 1137, 1221, 1283, 1590, 2181, 2937, 3044, 4335
EarI CTCTTC 8 cut(s) 1137, 1221, 1283, 1590, 2181, 2937, 3044, 4335
EciI GGCGGA 1 cut(s) 1271
Ecl136II GAGCTC 1 cut(s) 132
Eco130I CCWWGG 9 cut(s) 6, 493, 1046, 1605, 1866, 2120, 2309, 3500, 4071
Eco147I AGGCCT 1 cut(s) 2125
Eco24I GRGCYC 1 cut(s) 134
Eco31I GGTCTC 2 cut(s) 3375, 3886
Eco32I GATATC 2 cut(s) 3588, 4141
Eco47I GGWCC 5 cut(s) 160, 192, 2288, 2393, 3262
Eco53kI GAGCTC 1 cut(s) 132
Eco57I CTGAAG 7 cut(s) 1095, 2075, 2584, 2987, 3068, 3848, 3872
Eco81I CCTNAGG 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 2690
Eco91I GGTNACC 1 cut(s) 3761
EcoICRI GAGCTC 1 cut(s) 132
EcoNI CCTNNNNNAGG 2 cut(s) 1737, 2283
EcoO109I RGGNCCY 3 cut(s) 2288, 3671, 3962
EcoO65I GGTNACC 1 cut(s) 3761
EcoRI GAATTC 3 cut(s) 803, 1409, 2831
EcoRII CCWGG 9 cut(s) 638, 807, 1055, 2284, 2371, 2398, 2493, 2766, 3332
EcoRV GATATC 2 cut(s) 3588, 4141
EcoT14I CCWWGG 9 cut(s) 6, 493, 1046, 1605, 1866, 2120, 2309, 3500, 4071
EcoT22I ATGCAT 1 cut(s) 577
EcoT38I GRGCYC 1 cut(s) 134
EgeI GGCGCC 1 cut(s) 4164
EheI GGCGCC 1 cut(s) 4164
ErhI CCWWGG 9 cut(s) 6, 493, 1046, 1605, 1866, 2120, 2309, 3500, 4071
Esp3I CGTCTC 1 cut(s) 257
FalI AAGNNNNNCTT 8 cut(s) 477, 509, 2113, 2145, 2819, 2851, 2891, 2923
FaqI GGGAC 2 cut(s) 173, 1849
FauI CCCGC 1 cut(s) 2883
FauNDI CATATG 1 cut(s) 1297
FbaI TGATCA 4 cut(s) 696, 2074, 3202, 3996
FblI GTMKAC 3 cut(s) 2745, 2972, 3562
FriOI GRGCYC 1 cut(s) 134
FspBI CTAG 6 cut(s) 557, 677, 1611, 2582, 3377, 4284
GlaI GCGC 6 cut(s) 25, 41, 123, 216, 2753, 4164
GsaI CCCAGC 1 cut(s) 2758
GsuI CTGGAG 5 cut(s) 830, 1218, 3050, 3134, 3382
HaeII RGCGCY 3 cut(s) 43, 125, 4166
HapII CCGG 6 cut(s) 62, 95, 114, 201, 2180, 3464
HgaI GACGC 5 cut(s) 218, 245, 1252, 3548, 4085
HhaI GCGC 6 cut(s) 26, 42, 124, 217, 2754, 4165
Hin1I GRCGYC 2 cut(s) 210, 4163
Hin6I GCGC 6 cut(s) 24, 40, 122, 215, 2752, 4163
HinP1I GCGC 6 cut(s) 24, 40, 122, 215, 2752, 4163
HincII GTYRAC 1 cut(s) 3697
HindII GTYRAC 1 cut(s) 3697
HpaII CCGG 6 cut(s) 62, 95, 114, 201, 2180, 3464
Hpy166II GTNNAC 8 cut(s) 13, 1989, 2396, 2746, 2973, 3412, 3563, 3697
Hpy8I GTNNAC 8 cut(s) 13, 1989, 2396, 2746, 2973, 3412, 3563, 3697
Hpy99I CGWCG 4 cut(s) 212, 239, 388, 4246
HpyCH4IV ACGT 3 cut(s) 386, 396, 3184
HpySE526I ACGT 3 cut(s) 386, 396, 3184
Hsp92I GRCGYC 2 cut(s) 210, 4163
HspAI GCGC 6 cut(s) 24, 40, 122, 215, 2752, 4163
KasI GGCGCC 1 cut(s) 4162
KpnI GGTACC 1 cut(s) 3574
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Ksp22I TGATCA 4 cut(s) 696, 2074, 3202, 3996
LguI GCTCTTC 3 cut(s) 1137, 1221, 2181
LweI GCATC 7 cut(s) 466, 892, 2032, 2206, 3394, 4123, 4285
MabI ACCWGGT 1 cut(s) 2398
MaeI CTAG 6 cut(s) 557, 677, 1611, 2582, 3377, 4284
MaeII ACGT 3 cut(s) 386, 396, 3184
MaeIII GTNAC 5 cut(s) 1465, 2777, 2961, 3685, 3761
MbiI CCGCTC 1 cut(s) 1778
MfeI CAATTG 4 cut(s) 187, 942, 2684, 3449
MflI RGATCY 5 cut(s) 1302, 1906, 2002, 2116, 2675
MhlI GDGCHC 7 cut(s) 134, 396, 1507, 3112, 3655, 3808, 4122
MlsI TGGCCA 3 cut(s) 929, 3612, 4227
MluNI TGGCCA 3 cut(s) 929, 3612, 4227
Mly113I GGCGCC 1 cut(s) 4163
MlyI GAGTC 5 cut(s) 755, 2225, 2879, 3277, 3494
MmeI TCCRAC 5 cut(s) 126, 2326, 2622, 2865, 3901
Mox20I TGGCCA 3 cut(s) 929, 3612, 4227
Mph1103I ATGCAT 1 cut(s) 577
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 4 cut(s) 230, 1697, 2064, 4189
MscI TGGCCA 3 cut(s) 929, 3612, 4227
MslI CAYNNNNRTG 8 cut(s) 897, 1338, 2630, 3140, 3768, 4038, 4127, 4260
Msp20I TGGCCA 3 cut(s) 929, 3612, 4227
MspA1I CMGCKG 3 cut(s) 182, 1460, 2978
MspI CCGG 6 cut(s) 62, 95, 114, 201, 2180, 3464
MunI CAATTG 4 cut(s) 187, 942, 2684, 3449
Mva1269I GAATGC 3 cut(s) 1345, 1531, 3297
MvaI CCWGG 9 cut(s) 640, 809, 1057, 2286, 2373, 2400, 2495, 2768, 3334
MvnI CGCG 4 cut(s) 26, 215, 239, 2752
NaeI GCCGGC 1 cut(s) 202
NarI GGCGCC 1 cut(s) 4163
NciI CCSGG 3 cut(s) 62, 114, 2181
NcoI CCATGG 1 cut(s) 4071
NdeI CATATG 1 cut(s) 1297
NgoMIV GCCGGC 1 cut(s) 200
NheI GCTAGC 1 cut(s) 2581
NlaIV GGNNCC 9 cut(s) 161, 303, 1603, 2118, 2394, 3572, 3963, 4164, 4308
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 2 cut(s) 1465, 3761
NsiI ATGCAT 1 cut(s) 577
NspI RCATGY 4 cut(s) 2712, 3033, 3559, 4304
NspV TTCGAA 1 cut(s) 2134
OliI CACNNNNGTG 1 cut(s) 3768
PagI TCATGA 3 cut(s) 463, 564, 988
PaqCI CACCTGC 1 cut(s) 208
PceI AGGCCT 1 cut(s) 2125
PciI ACATGT 2 cut(s) 2708, 4300
PciSI GCTCTTC 3 cut(s) 1137, 1221, 2181
PcsI WCGNNNNNNNCGW 2 cut(s) 2611, 3181
PctI GAATGC 3 cut(s) 1345, 1531, 3297
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 4 cut(s) 230, 1697, 2064, 4189
PfeI GAWTC 9 cut(s) 151, 409, 489, 821, 1289, 1895, 3432, 4238, 4252
Pfl23II CGTACG 1 cut(s) 27
PflMI CCANNNNNTGG 4 cut(s) 343, 845, 1442, 4234
PfoI TCCNGGA 1 cut(s) 807
PleI GAGTC 5 cut(s) 754, 2225, 2878, 3276, 3493
PluTI GGCGCC 1 cut(s) 4166
PpsI GAGTC 5 cut(s) 754, 2225, 2878, 3276, 3493
PpuMI RGGWCCY 1 cut(s) 2288
PscI ACATGT 2 cut(s) 2708, 4300
PshAI GACNNNNGTC 3 cut(s) 2286, 2601, 3267
PshBI ATTAAT 4 cut(s) 1188, 1350, 2202, 3623
Psp124BI GAGCTC 1 cut(s) 134
Psp1406I AACGTT 1 cut(s) 3184
Psp5II RGGWCCY 1 cut(s) 2288
Psp6I CCWGG 9 cut(s) 638, 807, 1055, 2284, 2371, 2398, 2493, 2766, 3332
PspEI GGTNACC 1 cut(s) 3761
PspFI CCCAGC 1 cut(s) 2754
PspGI CCWGG 9 cut(s) 638, 807, 1055, 2284, 2371, 2398, 2493, 2766, 3332
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 9 cut(s) 161, 303, 1603, 2118, 2394, 3572, 3963, 4164, 4308
PspPI GGNCC 8 cut(s) 160, 192, 2288, 2393, 3262, 3330, 3671, 3962
PspPPI RGGWCCY 1 cut(s) 2288
PstNI CAGNNNCTG 1 cut(s) 1463
PsuI RGATCY 5 cut(s) 1302, 1906, 2002, 2116, 2675
PvuII CAGCTG 2 cut(s) 1460, 2978
RseI CAYNNNNRTG 8 cut(s) 897, 1338, 2630, 3140, 3768, 4038, 4127, 4260
SacI GAGCTC 1 cut(s) 134
SapI GCTCTTC 3 cut(s) 1137, 1221, 2181
Sau96I GGNCC 8 cut(s) 160, 192, 2288, 2393, 3262, 3330, 3671, 3962
ScaI AGTACT 1 cut(s) 1784
SchI GAGTC 5 cut(s) 755, 2225, 2879, 3277, 3494
SduI GDGCHC 7 cut(s) 134, 396, 1507, 3112, 3655, 3808, 4122
SexAI ACCWGGT 1 cut(s) 2398
SfaNI GCATC 7 cut(s) 466, 892, 2032, 2206, 3394, 4123, 4285
SfcI CTRYAG 2 cut(s) 2016, 2973
SfoI GGCGCC 1 cut(s) 4164
SfuI TTCGAA 1 cut(s) 2134
SinI GGWCC 5 cut(s) 160, 192, 2288, 2393, 3262
SmiMI CAYNNNNRTG 8 cut(s) 897, 1338, 2630, 3140, 3768, 4038, 4127, 4260
SpeI ACTAGT 1 cut(s) 4283
SseBI AGGCCT 1 cut(s) 2125
SspDI GGCGCC 1 cut(s) 4162
SspI AATATT 4 cut(s) 1441, 1846, 1922, 3594
SspMI CTAG 6 cut(s) 557, 677, 1611, 2582, 3377, 4284
SstI GAGCTC 1 cut(s) 134
StuI AGGCCT 1 cut(s) 2125
StyI CCWWGG 9 cut(s) 6, 493, 1046, 1605, 1866, 2120, 2309, 3500, 4071
TaiI ACGT 3 cut(s) 389, 399, 3187
TatI WGTACW 1 cut(s) 1782
TauI GCSGC 6 cut(s) 122, 182, 207, 215, 1982, 3834
TfiI GAWTC 9 cut(s) 151, 409, 489, 821, 1289, 1895, 3432, 4238, 4252
TscAI CASTG 9 cut(s) 610, 1143, 1426, 1708, 1810, 1991, 3547, 3988, 4182
TseFI GTSAC 2 cut(s) 1465, 3761
Tsp45I GTSAC 2 cut(s) 1465, 3761
TspGWI ACGGA 7 cut(s) 30, 281, 1295, 1364, 3278, 3617, 3645
TspRI CASTG 9 cut(s) 610, 1143, 1426, 1708, 1810, 1991, 3547, 3988, 4182
Van91I CCANNNNNTGG 4 cut(s) 343, 845, 1442, 4234
VpaK11BI GGWCC 5 cut(s) 160, 192, 2288, 2393, 3262
VspI ATTAAT 4 cut(s) 1188, 1350, 2202, 3623
XagI CCTNNNNNAGG 2 cut(s) 1737, 2283
XceI RCATGY 4 cut(s) 2712, 3033, 3559, 4304
XcmI CCANNNNNNNNNTGG 2 cut(s) 412, 4078
XmiI GTMKAC 3 cut(s) 2745, 2972, 3562
XmnI GAANNNNTTC 4 cut(s) 230, 1697, 2064, 4189
XspI CTAG 6 cut(s) 557, 677, 1611, 2582, 3377, 4284
ZrmI AGTACT 1 cut(s) 1784
Zsp2I ATGCAT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.