FvH4_7g05010

DNA repair REX1-B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008780
Physical Location & Seq
Reverse (-)
5442386 .. 5443818
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g05010.t1

Sequence Viewer

Length: 624 bp
ATGGAAGAATTTGAGAAGAAGTTATCAATCAGAGACGACAGCGACGGCGAAGAAGAAGCTAAGAAGAAGGTGTCCACCGTCGAATTGCTTCGCGAATTCCTTGGAATTCAGCAGCGCAGAGCAGAAGCATACGCCAAGCTCAGAATGGGGTTCGCCCGCTATATGGAGTCTTCGTCTTCCTCGGCCGAGTCCGCCTATCAAAAGCTTTGCGGCGACGTCACCCAGGAATTTAGTGATTGCTCGAGACAAGTGCTCCACTTGGAATCCCTCTTCCTGGGTCCTCATTATGACCGCCTTGATTTGGCCCACCTCCTCAGAGCTGTTCAAACCCACGAGAAGCAGAAACTGAACCTGACGGCTACACTTCAGCTGTTGAAGAAGGCCGGCCGACCCTCGGAGCGGCTGGTGAGTCATGAGAATTGCAGGTTTGAGAAGCCAATGGAGCATCAGTGTGTCCATCTCCATGAAATAACCGAAGCTGCTGGAACTGAGGAAGCTGAAGCCAATGCCCAGTATGATATTGATCTCAAGGAAGCCATTCGAGGGGTGCAGGACTCTGTCACTGCTATAAATGAACATTTGGAAGAAGTCAGATATGAAATTGCTGCCCATGAAGCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_repr_REX1B

Protein Analysis

208

Amino Acids

23.66

Weight (kDa)

5.27

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_repr_REX1B PF14966 28 - 126 6.1e-29 DNA repair REX1-B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 219
Acc36I ACCTGC 1 cut(s) 414
AccBSI CCGCTC 1 cut(s) 400
AccII CGCG 1 cut(s) 93
AciI CCGC 5 cut(s) 157, 192, 210, 292, 400
AcoI YGGCCR 2 cut(s) 183, 385
AcsI RAATTY 4 cut(s) 8, 95, 105, 227
AcuI CTGAAG 2 cut(s) 350, 519
AcyI GRCGYC 1 cut(s) 216
AfiI CCNNNNNNNGG 6 cut(s) 163, 274, 301, 394, 399, 543
AgsI TTSAA 2 cut(s) 326, 376
AjnI CCWGG 2 cut(s) 222, 273
AluBI AGCT 8 cut(s) 59, 139, 205, 320, 370, 479, 497, 617
AluI AGCT 8 cut(s) 59, 139, 205, 320, 370, 479, 497, 617
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 2 cut(s) 27, 238
AlwNI CAGNNNCTG 1 cut(s) 346
Ama87I CYCGRG 1 cut(s) 241
AoxI GGCC 4 cut(s) 183, 303, 381, 385
ApeKI GCWGC 3 cut(s) 112, 479, 605
ApoI RAATTY 4 cut(s) 8, 95, 105, 227
Asp700I GAANNNNTTC 2 cut(s) 87, 537
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 2 cut(s) 278, 304
AsuHPI GGTGA 2 cut(s) 211, 418
AvaI CYCGRG 1 cut(s) 241
AvaII GGWCC 1 cut(s) 278
BauI CACGAG 1 cut(s) 332
BbsI GAAGAC 2 cut(s) 162, 168
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 3 cut(s) 124, 466, 592
BccI CCATC 1 cut(s) 465
BceAI ACGGC 2 cut(s) 61, 372
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 2 cut(s) 224, 275
BcoDI GTCTC 2 cut(s) 27, 238
BfuAI ACCTGC 1 cut(s) 414
BisI GCNGC 5 cut(s) 113, 211, 401, 480, 606
BlsI GCNGC 5 cut(s) 114, 212, 402, 481, 607
Bme1390I CCNGG 2 cut(s) 224, 275
Bme18I GGWCC 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 241
BmgT120I GGNCC 2 cut(s) 278, 304
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 2 cut(s) 224, 275
BmrI ACTGGG 1 cut(s) 505
BmsI GCATC 1 cut(s) 454
BmuI ACTGGG 1 cut(s) 505
BpiI GAAGAC 2 cut(s) 162, 168
BpuEI CTTGAG 1 cut(s) 512
BsaBI GATNNNNATC 1 cut(s) 522
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 5 cut(s) 100, 180, 222, 274, 393
BsaXI ACNNNNNCTCC 6 cut(s) 158, 188, 237, 267, 434, 464
Bsc4I CCNNNNNNNGG 6 cut(s) 163, 274, 301, 394, 399, 543
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 1 cut(s) 511
Bse8I GATNNNNATC 1 cut(s) 522
BseBI CCWGG 2 cut(s) 224, 275
BseDI CCNNGG 5 cut(s) 100, 180, 222, 274, 393
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 6 cut(s) 163, 274, 301, 394, 399, 543
BseMII CTCAG 3 cut(s) 154, 328, 480
BseNI ACTGG 1 cut(s) 511
BseRI GAGGAG 1 cut(s) 302
BseX3I CGGCCG 2 cut(s) 183, 385
BseXI GCAGC 3 cut(s) 124, 466, 592
BsgI GTGCAG 1 cut(s) 569
Bsh1236I CGCG 1 cut(s) 93
Bsh1285I CGRYCG 2 cut(s) 186, 388
BshFI GGCC 4 cut(s) 185, 305, 383, 387
BsiEI CGRYCG 2 cut(s) 186, 388
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 1 cut(s) 384
BslI CCNNNNNNNGG 6 cut(s) 163, 274, 301, 394, 399, 543
BsmAI GTCTC 2 cut(s) 27, 238
BsmBI CGTCTC 1 cut(s) 27
BsnI GGCC 4 cut(s) 185, 305, 383, 387
BsoBI CYCGRG 1 cut(s) 241
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 1 cut(s) 523
Bsp68I TCGCGA 1 cut(s) 93
BspACI CCGC 5 cut(s) 157, 192, 210, 292, 400
BspANI GGCC 4 cut(s) 185, 305, 383, 387
BspCNI CTCAG 3 cut(s) 153, 327, 481
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 1 cut(s) 279
BspMI ACCTGC 1 cut(s) 414
BsrBI CCGCTC 1 cut(s) 400
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 1 cut(s) 511
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 5 cut(s) 100, 180, 222, 274, 393
BssMI GATC 1 cut(s) 523
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 332
BssT1I CCWWGG 1 cut(s) 100
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 2 cut(s) 224, 275
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 275
BstACI GRCGYC 1 cut(s) 216
BstC8I GCNNGC 2 cut(s) 157, 385
BstDEI CTNAG 4 cut(s) 60, 140, 314, 489
BstFNI CGCG 1 cut(s) 93
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 1 cut(s) 526
BstMAI GTCTC 2 cut(s) 27, 238
BstMBI GATC 1 cut(s) 523
BstMCI CGRYCG 2 cut(s) 186, 388
BstMWI GCNNNNNNNGC 3 cut(s) 191, 442, 614
BstNI CCWGG 2 cut(s) 224, 275
BstSCI CCNGG 2 cut(s) 222, 273
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 3 cut(s) 124, 466, 592
BstV2I GAAGAC 2 cut(s) 162, 168
BstZI CGGCCG 2 cut(s) 183, 385
BsuRI GGCC 4 cut(s) 185, 305, 383, 387
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 2 cut(s) 455, 561
BtuMI TCGCGA 1 cut(s) 93
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 2 cut(s) 157, 385
CaiI CAGNNNCTG 1 cut(s) 346
CciI TCATGA 1 cut(s) 412
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 278, 304
CviAII CATG 3 cut(s) 413, 464, 611
DdeI CTNAG 4 cut(s) 60, 140, 314, 489
DpnI GATC 1 cut(s) 525
DpnII GATC 1 cut(s) 523
EaeI YGGCCR 2 cut(s) 183, 385
EagI CGGCCG 2 cut(s) 183, 385
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
EciI GGCGGA 1 cut(s) 181
EclXI CGGCCG 2 cut(s) 183, 385
Eco130I CCWWGG 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 278
Eco52I CGGCCG 2 cut(s) 183, 385
Eco57I CTGAAG 2 cut(s) 350, 519
Eco88I CYCGRG 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 278
EcoRI GAATTC 2 cut(s) 95, 105
EcoRII CCWGG 2 cut(s) 222, 273
EcoT14I CCWWGG 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 100
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 3 cut(s) 416, 467, 614
FatI CATG 3 cut(s) 412, 463, 610
FauI CCCGC 1 cut(s) 164
Fnu4HI GCNGC 5 cut(s) 113, 211, 401, 480, 606
FseI GGCCGGCC 1 cut(s) 387
Fsp4HI GCNGC 5 cut(s) 113, 211, 401, 480, 606
GlaI GCGC 1 cut(s) 116
GluI GCNGC 5 cut(s) 113, 211, 401, 480, 606
HaeIII GGCC 4 cut(s) 185, 305, 383, 387
HapII CCGG 1 cut(s) 384
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 3 cut(s) 416, 467, 614
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 5 cut(s) 167, 188, 263, 409, 554
HpaII CCGG 1 cut(s) 384
HphI GGTGA 2 cut(s) 211, 418
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 5 cut(s) 32, 143, 317, 397, 593
Hpy188III TCNNGA 3 cut(s) 92, 243, 413
Hpy8I GTNNAC 1 cut(s) 75
Hpy99I CGWCG 3 cut(s) 47, 83, 218
HpyAV CCTTC 2 cut(s) 61, 373
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 2 cut(s) 423, 550
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 442, 614
HpyF3I CTNAG 4 cut(s) 60, 140, 314, 489
HpySE526I ACGT 1 cut(s) 216
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 3 cut(s) 416, 467, 614
HspAI GCGC 1 cut(s) 115
KroI GCCGGC 1 cut(s) 383
KroNI GCCGGC 1 cut(s) 385
Kzo9I GATC 1 cut(s) 523
LmnI GCTCC 3 cut(s) 258, 397, 442
Lsp1109I GCAGC 3 cut(s) 124, 466, 592
LweI GCATC 1 cut(s) 454
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 2 cut(s) 217, 559
MalI GATC 1 cut(s) 525
MbiI CCGCTC 1 cut(s) 400
MboI GATC 1 cut(s) 523
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 7 cut(s) 8, 83, 95, 105, 227, 418, 600
MlyI GAGTC 4 cut(s) 176, 197, 418, 548
MnlI CCTC 8 cut(s) 190, 278, 291, 320, 323, 403, 484, 536
MroNI GCCGGC 1 cut(s) 383
MroXI GAANNNNTTC 2 cut(s) 87, 537
MslI CAYNNNNRTG 2 cut(s) 450, 462
MspA1I CMGCKG 1 cut(s) 370
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 2 cut(s) 224, 275
MvaI CCWGG 2 cut(s) 224, 275
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 191, 442, 614
NaeI GCCGGC 1 cut(s) 385
NdeII GATC 1 cut(s) 523
NgoMIV GCCGGC 1 cut(s) 383
NlaIII CATG 3 cut(s) 416, 467, 614
NlaIV GGNNCC 1 cut(s) 279
NmeAIII GCCGAG 2 cut(s) 161, 211
NmuCI GTSAC 2 cut(s) 217, 559
NruI TCGCGA 1 cut(s) 93
PaeR7I CTCGAG 1 cut(s) 241
PagI TCATGA 1 cut(s) 412
PdiI GCCGGC 1 cut(s) 385
PdmI GAANNNNTTC 2 cut(s) 87, 537
PfeI GAWTC 1 cut(s) 263
PflFI GACNNNGTC 1 cut(s) 557
PkrI GCNGC 5 cut(s) 114, 212, 402, 481, 607
PleI GAGTC 4 cut(s) 175, 196, 417, 548
PpsI GAGTC 4 cut(s) 175, 196, 417, 548
PpuMI RGGWCCY 1 cut(s) 278
Psp5II RGGWCCY 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 222, 273
PspGI CCWGG 2 cut(s) 222, 273
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 2 cut(s) 278, 304
PspPPI RGGWCCY 1 cut(s) 278
PstNI CAGNNNCTG 1 cut(s) 346
PsyI GACNNNGTC 1 cut(s) 557
PvuII CAGCTG 1 cut(s) 370
RigI GGCCGGCC 1 cut(s) 387
RruI TCGCGA 1 cut(s) 93
RseI CAYNNNNRTG 2 cut(s) 450, 462
SatI GCNGC 5 cut(s) 113, 211, 401, 480, 606
Sau3AI GATC 1 cut(s) 523
Sau96I GGNCC 2 cut(s) 278, 304
SchI GAGTC 4 cut(s) 176, 197, 418, 548
ScrFI CCNGG 2 cut(s) 224, 275
SduI GDGCHC 1 cut(s) 255
SfaNI GCATC 1 cut(s) 454
Sfr274I CTCGAG 1 cut(s) 241
SinI GGWCC 1 cut(s) 278
SlaI CTCGAG 1 cut(s) 241
SmiMI CAYNNNNRTG 2 cut(s) 450, 462
SmlI CTYRAG 2 cut(s) 241, 527
SmoI CTYRAG 2 cut(s) 241, 527
Sse9I AATT 7 cut(s) 8, 83, 95, 105, 227, 418, 600
SsiI CCGC 5 cut(s) 157, 192, 210, 292, 400
StyD4I CCNGG 2 cut(s) 222, 273
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 1 cut(s) 219
TaqI TCGA 3 cut(s) 81, 242, 541
TasI AATT 7 cut(s) 8, 83, 95, 105, 227, 418, 600
TauI GCSGC 2 cut(s) 213, 403
TfiI GAWTC 1 cut(s) 263
TscAI CASTG 2 cut(s) 455, 568
TseFI GTSAC 2 cut(s) 217, 559
TseI GCWGC 3 cut(s) 112, 479, 605
Tsp45I GTSAC 2 cut(s) 217, 559
TspDTI ATGAA 3 cut(s) 480, 588, 612
TspRI CASTG 2 cut(s) 455, 568
Tth111I GACNNNGTC 1 cut(s) 557
VpaK11BI GGWCC 1 cut(s) 278
XapI RAATTY 4 cut(s) 8, 95, 105, 227
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XhoI CTCGAG 1 cut(s) 241
XmnI GAANNNNTTC 2 cut(s) 87, 537
ZraI GACGTC 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.