MD02G1265000.v1.1

DNA repair REX1-B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008780
Physical Location & Seq
Forward (+)
31918480 .. 31920231
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1265000.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGAAGAATTTGACAAGAAGGTATCAATCACAGACCCAATGGAAGACGAAAGTGTGGCATCTAAAACAAAAACCGTCGGTTTGCTCCGCGAGTTCCTCGGAATTCAGCAGCGGAGGGCGGAGGCCTACGCCAAGCTGAAACGAGGGTTTGGGGAGTATATAGAGTCGTCGCCGCCGTCGTCGGGAGTAGATGCGGAGTTGGCGTACCAGCAAGTTTGCAGTGAGGTGACGCTCGAATTCAACGAGTGCTCGAAGCAAGTTCTTCGGATCGAATCGCTGTTCCTCAACGATCCCGATTTCTCCCGGATTGATTTAGCCCATCTCCTCAGAGCTGTTCAGACCCACGAGAAGCAGAAACTGAATTTGACGGTGACAATTCAGGTGTTGAAGAGGGCTGGTAGACCCTCAGAGCGTCTGGTGAGTCATGAAAATTGCAGATTTAACAGACCAATGGAGCATGAGTGCGTGCATCTCCACGAGATAACAGAAGCTGCCGGAACTGAAGAAGCAGAAGCGAATGCCCAGTATGATAATGATCTCAAGGAGGCCATTAGAGGGGTGCAGGATGCTGTGACCGCTATAAATGAGCATTTGGAAGAAGTCAGATATGAAATTGCTGCCCTTGAAACCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_repr_REX1B

Protein Analysis

212

Amino Acids

23.96

Weight (kDa)

4.96

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_repr_REX1B PF14966 28 - 130 4.6e-28 DNA repair REX1-B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 400
AccII CGCG 1 cut(s) 90
AciI CCGC 6 cut(s) 88, 112, 119, 173, 194, 576
AclWI GGATC 2 cut(s) 275, 284
AcsI RAATTY 4 cut(s) 8, 102, 236, 361
AcuI CTGAAG 1 cut(s) 522
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 2 cut(s) 182, 555
AgsI TTSAA 3 cut(s) 241, 388, 626
AluBI AGCT 3 cut(s) 136, 332, 491
AluI AGCT 3 cut(s) 136, 332, 491
Alw21I GWGCWC 1 cut(s) 251
AlwI GGATC 2 cut(s) 275, 284
AlwNI CAGNNNCTG 2 cut(s) 358, 491
AoxI GGCC 2 cut(s) 123, 546
ApeKI GCWGC 3 cut(s) 109, 491, 617
ApoI RAATTY 4 cut(s) 8, 102, 236, 361
AsuC2I CCSGG 1 cut(s) 304
AsuHPI GGTGA 3 cut(s) 238, 382, 430
BauI CACGAG 2 cut(s) 344, 476
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 3 cut(s) 121, 478, 604
BccI CCATC 1 cut(s) 327
BceAI ACGGC 1 cut(s) 160
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BcnI CCSGG 1 cut(s) 304
BisI GCNGC 4 cut(s) 110, 173, 492, 618
BlsI GCNGC 4 cut(s) 111, 174, 493, 619
Bme1390I CCNGG 1 cut(s) 304
BmrFI CCNGG 1 cut(s) 304
BmrI ACTGGG 1 cut(s) 517
BmsI GCATC 4 cut(s) 68, 181, 478, 556
BmuI ACTGGG 1 cut(s) 517
BpiI GAAGAC 1 cut(s) 51
BpuEI CTTGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 304
BsaBI GATNNNNATC 1 cut(s) 534
BsaJI CCNNGG 1 cut(s) 97
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 446, 476
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 555
Bse1I ACTGG 1 cut(s) 523
Bse8I GATNNNNATC 1 cut(s) 534
BseDI CCNNGG 1 cut(s) 97
BseGI GGATG 1 cut(s) 571
BseJI GATNNNNATC 1 cut(s) 534
BseLI CCNNNNNNNGG 2 cut(s) 182, 555
BseMII CTCAG 2 cut(s) 340, 420
BseNI ACTGG 1 cut(s) 523
BseRI GAGGAG 1 cut(s) 314
BseXI GCAGC 3 cut(s) 121, 478, 604
BsgI GTGCAG 1 cut(s) 581
Bsh1236I CGCG 1 cut(s) 90
BshFI GGCC 2 cut(s) 125, 548
BsiHKAI GWGCWC 1 cut(s) 251
BsiSI CCGG 2 cut(s) 304, 495
BslI CCNNNNNNNGG 2 cut(s) 182, 555
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 2 cut(s) 125, 548
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 3 cut(s) 267, 289, 535
BspACI CCGC 6 cut(s) 88, 112, 119, 173, 194, 576
BspANI GGCC 2 cut(s) 125, 548
BspCNI CTCAG 2 cut(s) 339, 419
BspFNI CGCG 1 cut(s) 90
BspHI TCATGA 1 cut(s) 424
BspPI GGATC 2 cut(s) 275, 284
BsrI ACTGG 1 cut(s) 523
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 3 cut(s) 267, 289, 535
BssSI CACGAG 2 cut(s) 344, 476
Bst2BI CACGAG 2 cut(s) 344, 476
Bst4CI ACNGT 2 cut(s) 76, 370
Bst6I CTCTTC 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 467
BstDEI CTNAG 2 cut(s) 326, 406
BstF5I GGATG 1 cut(s) 571
BstFNI CGCG 1 cut(s) 90
BstKTI GATC 3 cut(s) 270, 292, 538
BstMBI GATC 3 cut(s) 267, 289, 535
BstMWI GCNNNNNNNGC 2 cut(s) 200, 575
BstSCI CCNGG 1 cut(s) 302
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 121, 478, 604
BstV2I GAAGAC 1 cut(s) 51
BsuRI GGCC 2 cut(s) 125, 548
BtsCI GGATG 1 cut(s) 571
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 1 cut(s) 226
Cac8I GCNNGC 1 cut(s) 467
CaiI CAGNNNCTG 2 cut(s) 358, 491
CciI TCATGA 1 cut(s) 424
CseI GACGC 2 cut(s) 238, 401
Csp6I GTAC 1 cut(s) 205
CviAII CATG 2 cut(s) 425, 458
CviJI RGCY 7 cut(s) 125, 136, 317, 332, 395, 491, 548
CviKI_1 RGCY 7 cut(s) 125, 136, 317, 332, 395, 491, 548
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 2 cut(s) 326, 406
DpnI GATC 3 cut(s) 269, 291, 537
DpnII GATC 3 cut(s) 267, 289, 535
Eam1104I CTCTTC 1 cut(s) 383
EarI CTCTTC 1 cut(s) 383
EciI GGCGGA 1 cut(s) 134
Eco147I AGGCCT 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 522
EcoRI GAATTC 2 cut(s) 102, 236
FaeI CATG 2 cut(s) 428, 461
FaiI YATR 7 cut(s) 159, 161, 426, 459, 528, 581, 609
FatI CATG 2 cut(s) 424, 457
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 4 cut(s) 110, 173, 492, 618
FokI GGATG 1 cut(s) 578
Fsp4HI GCNGC 4 cut(s) 110, 173, 492, 618
GluI GCNGC 4 cut(s) 110, 173, 492, 618
HaeIII GGCC 2 cut(s) 125, 548
HapII CCGG 2 cut(s) 304, 495
HgaI GACGC 2 cut(s) 238, 401
Hin1II CATG 2 cut(s) 428, 461
HinfI GANTC 3 cut(s) 164, 272, 421
HpaII CCGG 2 cut(s) 304, 495
HphI GGTGA 3 cut(s) 238, 382, 430
Hpy166II GTNNAC 1 cut(s) 401
Hpy188I TCNGA 6 cut(s) 101, 267, 329, 339, 409, 605
Hpy188III TCNNGA 3 cut(s) 183, 293, 425
Hpy8I GTNNAC 1 cut(s) 401
Hpy99I CGWCG 4 cut(s) 80, 172, 181, 184
HpyAV CCTTC 1 cut(s) 13
HpyCH4III ACNGT 2 cut(s) 76, 370
HpyCH4V TGCA 4 cut(s) 219, 435, 469, 562
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 575
HpyF3I CTNAG 2 cut(s) 326, 406
Hsp92II CATG 2 cut(s) 428, 461
Kzo9I GATC 3 cut(s) 267, 289, 535
LmnI GCTCC 2 cut(s) 90, 454
LpnPI CCDG 8 cut(s) 221, 317, 365, 381, 401, 508, 536, 548
Lsp1109I GCAGC 3 cut(s) 121, 478, 604
LweI GCATC 4 cut(s) 68, 181, 478, 556
MaeIII GTNAC 3 cut(s) 226, 370, 571
MalI GATC 3 cut(s) 269, 291, 537
MboI GATC 3 cut(s) 267, 289, 535
MboII GAAGA 6 cut(s) 17, 56, 254, 400, 515, 608
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 7 cut(s) 8, 102, 236, 361, 375, 430, 612
MlyI GAGTC 2 cut(s) 173, 430
MseI TTAA 1 cut(s) 441
MspA1I CMGCKG 1 cut(s) 112
MspI CCGG 2 cut(s) 304, 495
MspR9I CCNGG 1 cut(s) 304
Mva1269I GAATGC 1 cut(s) 523
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 2 cut(s) 200, 575
NciI CCSGG 1 cut(s) 304
NdeII GATC 3 cut(s) 267, 289, 535
NlaIII CATG 2 cut(s) 428, 461
NmuCI GTSAC 3 cut(s) 226, 370, 571
PagI TCATGA 1 cut(s) 424
PceI AGGCCT 1 cut(s) 125
PcsI WCGNNNNNNNCGW 3 cut(s) 173, 176, 240
PctI GAATGC 1 cut(s) 523
PfeI GAWTC 1 cut(s) 272
PfoI TCCNGGA 1 cut(s) 302
PkrI GCNGC 4 cut(s) 111, 174, 493, 619
PleI GAGTC 2 cut(s) 172, 429
PpsI GAGTC 2 cut(s) 172, 429
PstNI CAGNNNCTG 2 cut(s) 358, 491
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 4 cut(s) 110, 173, 492, 618
Sau3AI GATC 3 cut(s) 267, 289, 535
SchI GAGTC 2 cut(s) 173, 430
ScrFI CCNGG 1 cut(s) 304
SduI GDGCHC 1 cut(s) 251
SetI ASST 6 cut(s) 24, 138, 228, 334, 384, 493
SfaNI GCATC 4 cut(s) 68, 181, 478, 556
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 7 cut(s) 8, 102, 236, 361, 375, 430, 612
SseBI AGGCCT 1 cut(s) 125
SsiI CCGC 6 cut(s) 88, 112, 119, 173, 194, 576
StuI AGGCCT 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 302
TaaI ACNGT 2 cut(s) 76, 370
TaqI TCGA 3 cut(s) 234, 251, 270
TasI AATT 7 cut(s) 8, 102, 236, 361, 375, 430, 612
TauI GCSGC 1 cut(s) 175
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 3 cut(s) 226, 370, 571
TseI GCWGC 3 cut(s) 109, 491, 617
Tsp45I GTSAC 3 cut(s) 226, 370, 571
TspDTI ATGAA 2 cut(s) 441, 624
TspRI CASTG 1 cut(s) 226
XapI RAATTY 4 cut(s) 8, 102, 236, 361
XmiI GTMKAC 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.