FvH4_7g10321
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014796
Physical Location & Seq
Reverse (-)
9790882 .. 9792026
1145 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g10321.t1

Sequence Viewer

Length: 534 bp
ATGGAAGCTGGTGATCATCAAGAGGGAGCAGCGAGCTCATCATCCTCATCATCGAAAACTAGTGGCAGTTCCAGAACTTCATCATCAACAACATCCTTCACTAGTGCACTGACTCTCTCCCAACTGGGGGATGCTCATGTGCCTCAACGCTATGTTCTCCCTCCTTCCCAACGACCAATTAGTCCTTCTTATCCAAACCTTTCAACTCTTCCCATTCTTGACCTTTCCTCTTTGCAAAGTTCTTCTCTTAGGCCTCATGTCATCAACAATATTCAGACTGCTTGCAAGGAAATAGGATTCTTTCAGGTTATCAACCATGGAATCCCTCTTTCGGTGATGAAAGATGCCCTAAGTGCTGCGAATGAGTTCTTTAACCTACCTGTTGAGGACAAGATGCTTTTAGGGTCTGATAATGTTCATGCACCTGTAAGATATGGAACAAGCATAAATCATGCTGTTGATAAGGGTCACAAGGTTGTTGTGGAAATTCACTGCAACGCTGCAAAGGCTCACCATTTTGCAGAATATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

178

Amino Acids

18.94

Weight (kDa)

6.53

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 70 - 155 1.4e-22 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 180
AcsI RAATTY 1 cut(s) 486
AfiI CCNNNNNNNGG 3 cut(s) 126, 127, 331
AgsI TTSAA 1 cut(s) 204
AhlI ACTAGT 2 cut(s) 59, 101
AjuI GAANNNNNNNTTGG 2 cut(s) 169, 201
AluBI AGCT 2 cut(s) 8, 36
AluI AGCT 2 cut(s) 8, 36
Alw21I GWGCWC 2 cut(s) 38, 109
Alw44I GTGCAC 1 cut(s) 105
AoxI GGCC 1 cut(s) 251
ApaLI GTGCAC 1 cut(s) 105
ApeKI GCWGC 3 cut(s) 29, 356, 500
ApoI RAATTY 1 cut(s) 486
Asp700I GAANNNNTTC 1 cut(s) 365
AsuHPI GGTGA 3 cut(s) 23, 346, 503
BaeGI GKGCMC 1 cut(s) 109
BanII GRGCYC 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 38, 109
BbvI GCAGC 3 cut(s) 41, 343, 487
BclI TGATCA 1 cut(s) 13
BcuI ACTAGT 2 cut(s) 59, 101
BfaI CTAG 3 cut(s) 60, 102, 532
BisI GCNGC 3 cut(s) 30, 357, 501
BlsI GCNGC 3 cut(s) 31, 358, 502
BmrI ACTGGG 1 cut(s) 134
BmsI GCATC 3 cut(s) 121, 334, 384
BmuI ACTGGG 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 316
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 127, 331
Bse1I ACTGG 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 3 cut(s) 41, 92, 136
BseLI CCNNNNNNNGG 3 cut(s) 126, 127, 331
BseNI ACTGG 1 cut(s) 129
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 3 cut(s) 41, 343, 487
BshFI GGCC 1 cut(s) 253
BsiHKAI GWGCWC 2 cut(s) 38, 109
BslI CCNNNNNNNGG 3 cut(s) 126, 127, 331
BsnI GGCC 1 cut(s) 253
Bsp1286I GDGCHC 2 cut(s) 38, 109
Bsp143I GATC 1 cut(s) 13
Bsp19I CCATGG 1 cut(s) 316
BspANI GGCC 1 cut(s) 253
BsrI ACTGG 1 cut(s) 129
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 1 cut(s) 13
BssT1I CCWWGG 1 cut(s) 316
Bst6I CTCTTC 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 34, 283
BstDEI CTNAG 2 cut(s) 248, 350
BstDSI CCRYGG 1 cut(s) 316
BstF5I GGATG 3 cut(s) 41, 92, 136
BstKTI GATC 1 cut(s) 16
BstMBI GATC 1 cut(s) 13
BstMWI GCNNNNNNNGC 2 cut(s) 353, 506
BstSLI GKGCMC 1 cut(s) 109
BstV1I GCAGC 3 cut(s) 41, 343, 487
BsuRI GGCC 1 cut(s) 253
BtgI CCRYGG 1 cut(s) 316
BtsCI GGATG 3 cut(s) 41, 92, 136
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 2 cut(s) 107, 490
Cac8I GCNNGC 2 cut(s) 34, 283
CviAII CATG 5 cut(s) 137, 257, 317, 419, 452
CviJI RGCY 4 cut(s) 8, 36, 253, 509
CviKI_1 RGCY 4 cut(s) 8, 36, 253, 509
DdeI CTNAG 2 cut(s) 248, 350
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
DrdI GACNNNNNNGTC 1 cut(s) 180
DseDI GACNNNNNNGTC 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 316
Eco147I AGGCCT 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 38
Eco53kI GAGCTC 1 cut(s) 36
EcoICRI GAGCTC 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 5 cut(s) 140, 260, 320, 422, 455
FaiI YATR 9 cut(s) 138, 153, 258, 318, 420, 435, 446, 453, 528
FatI CATG 5 cut(s) 136, 256, 316, 418, 451
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 30, 357, 501
FokI GGATG 3 cut(s) 28, 79, 143
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 3 cut(s) 30, 357, 501
FspBI CTAG 3 cut(s) 60, 102, 532
GluI GCNGC 3 cut(s) 30, 357, 501
HaeIII GGCC 1 cut(s) 253
Hin1II CATG 5 cut(s) 140, 260, 320, 422, 455
HinfI GANTC 3 cut(s) 112, 297, 321
HphI GGTGA 3 cut(s) 23, 346, 503
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 2 cut(s) 276, 409
Hpy188III TCNNGA 3 cut(s) 20, 72, 218
Hpy8I GTNNAC 1 cut(s) 107
HpyAV CCTTC 3 cut(s) 106, 174, 195
HpyCH4V TGCA 7 cut(s) 107, 235, 285, 422, 495, 503, 521
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 506
HpyF3I CTNAG 2 cut(s) 248, 350
Hsp92II CATG 5 cut(s) 140, 260, 320, 422, 455
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 1 cut(s) 13
LmnI GCTCC 1 cut(s) 26
LpnPI CCDG 5 cut(s) 85, 110, 290, 393, 438
Lsp1109I GCAGC 3 cut(s) 41, 343, 487
LweI GCATC 3 cut(s) 121, 334, 384
MaeI CTAG 3 cut(s) 60, 102, 532
MaeIII GTNAC 1 cut(s) 467
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 2 cut(s) 200, 234
MhlI GDGCHC 2 cut(s) 38, 109
MluCI AATT 2 cut(s) 177, 486
MlyI GAGTC 1 cut(s) 106
MnlI CCTC 8 cut(s) 16, 55, 153, 171, 238, 264, 336, 379
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 1 cut(s) 372
MwoI GCNNNNNNNGC 2 cut(s) 353, 506
NcoI CCATGG 1 cut(s) 316
NdeII GATC 1 cut(s) 13
NlaIII CATG 5 cut(s) 140, 260, 320, 422, 455
NmuCI GTSAC 1 cut(s) 467
PceI AGGCCT 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 2 cut(s) 297, 321
PkrI GCNGC 3 cut(s) 31, 358, 502
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
Psp124BI GAGCTC 1 cut(s) 38
SacI GAGCTC 1 cut(s) 38
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 3 cut(s) 30, 357, 501
Sau3AI GATC 1 cut(s) 13
SchI GAGTC 1 cut(s) 106
SduI GDGCHC 2 cut(s) 38, 109
SetI ASST 9 cut(s) 10, 38, 201, 225, 309, 378, 382, 427, 477
SfaNI GCATC 3 cut(s) 121, 334, 384
SpeI ACTAGT 2 cut(s) 59, 101
Sse9I AATT 2 cut(s) 177, 486
SseBI AGGCCT 1 cut(s) 253
SspI AATATT 1 cut(s) 271
SspMI CTAG 3 cut(s) 60, 102, 532
SstI GAGCTC 1 cut(s) 38
StuI AGGCCT 1 cut(s) 253
StyI CCWWGG 1 cut(s) 316
TaqI TCGA 1 cut(s) 53
TasI AATT 2 cut(s) 177, 486
TfiI GAWTC 2 cut(s) 297, 321
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 2 cut(s) 114, 497
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 3 cut(s) 29, 356, 500
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 3 cut(s) 69, 353, 407
TspRI CASTG 2 cut(s) 114, 497
VneI GTGCAC 1 cut(s) 105
XapI RAATTY 1 cut(s) 486
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 3 cut(s) 60, 102, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.