pycom01g05520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014796
Physical Location & Seq
Forward (+)
5292105 .. 5292869
765 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05520.1

Sequence Viewer

Length: 576 bp
ATGACTGAACGTTACGTCCTGCTGTGCCTGAGATATCACCGGTTCTCCGGTGTGGCGATCCCCAGATCCAACCAGAGTGTTTTTCACAAGCAAATTGGGAACAGTCGGCAGAGTGACCGAGTCGGGAAATTTTGGAACCAGAACAGTCGTGTATCCCTTATCGAATCCGAACAGGAAATCGGGGTTTGTCGGCGAGGCCATGACTGGAGCCGTCGGAAAGGATCCATCGGGAGACCAGATCTAAACCGAATTCAAAACTTCAATGAACCACTGGCGCTCGTACTCCACCATCATATGGTTAAGGTCCTCGTCGTTGGTGATAGAAATAAGAGCGTCGAGATCTTAGCCGGGAAGCTGATACTTGAAGCAAACAGTGTCGTTAGGGGTGTTGTAGAGGGAGGAGAGCTTGGAGATGAAAGCGGAGAGCATGATATTGCGATCGAAGACAAGGATTTTGGTGTCACCGCCAACATAGGCGAGCTGGTGGTCGTGGGGGCAGGGCTGGACTTTGCCACTGTAACTGCACATGAACTTGACTTTGTAGGTGAAGGTTGTGGAGGAGGGTTAGTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

20.99

Weight (kDa)

6.24

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 295
AciI CCGC 2 cut(s) 420, 465
AclI AACGTT 1 cut(s) 10
AclWI GGATC 4 cut(s) 52, 60, 216, 229
AcsI RAATTY 2 cut(s) 128, 249
AfaI GTAC 1 cut(s) 282
AfiI CCNNNNNNNGG 1 cut(s) 295
AgeI ACCGGT 1 cut(s) 39
AgsI TTSAA 3 cut(s) 254, 262, 365
AloI GAACNNNNNNTCC 1 cut(s) 32
AluBI AGCT 3 cut(s) 355, 406, 481
AluI AGCT 3 cut(s) 355, 406, 481
Alw26I GTCTC 1 cut(s) 226
AlwI GGATC 4 cut(s) 52, 60, 216, 229
AoxI GGCC 1 cut(s) 196
ApoI RAATTY 2 cut(s) 128, 249
ArsI GACNNNNNNTTYG 2 cut(s) 437, 469
AsiGI ACCGGT 1 cut(s) 39
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 1 cut(s) 304
AsuC2I CCSGG 1 cut(s) 349
AsuHPI GGTGA 4 cut(s) 29, 329, 454, 557
AvaII GGWCC 1 cut(s) 304
BamHI GGATCC 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 450
BccI CCATC 2 cut(s) 233, 297
BceAI ACGGC 1 cut(s) 195
BciVI GTATCC 1 cut(s) 163
BcnI CCSGG 1 cut(s) 349
BcoDI GTCTC 1 cut(s) 226
BfoI RGCGCY 1 cut(s) 278
BfuI GTATCC 1 cut(s) 163
BglII AGATCT 2 cut(s) 238, 339
Bme1390I CCNGG 1 cut(s) 349
Bme18I GGWCC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 304
BmiI GGNNCC 3 cut(s) 137, 209, 223
BmrFI CCNGG 1 cut(s) 349
BpiI GAAGAC 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 226
BpuMI CCSGG 1 cut(s) 349
BsaI GGTCTC 1 cut(s) 226
BsaWI WCCGGW 2 cut(s) 39, 47
BsaXI ACNNNNNCTCC 3 cut(s) 29, 59, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse118I RCCGGY 1 cut(s) 39
Bse1I ACTGG 2 cut(s) 209, 276
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 2 cut(s) 209, 276
BseRI GAGGAG 2 cut(s) 414, 573
BsgI GTGCAG 1 cut(s) 507
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 1 cut(s) 198
BshTI ACCGGT 1 cut(s) 39
BsiEI CGRYCG 1 cut(s) 441
BsiSI CCGG 3 cut(s) 40, 48, 348
BslI CCNNNNNNNGG 1 cut(s) 295
BsmAI GTCTC 1 cut(s) 226
BsnI GGCC 1 cut(s) 198
Bso31I GGTCTC 1 cut(s) 226
Bsp143I GATC 6 cut(s) 57, 65, 221, 238, 339, 438
BspACI CCGC 2 cut(s) 420, 465
BspANI GGCC 1 cut(s) 198
BspCNI CTCAG 1 cut(s) 21
BspLI GGNNCC 3 cut(s) 137, 209, 223
BspPI GGATC 4 cut(s) 52, 60, 216, 229
BspTNI GGTCTC 1 cut(s) 226
BsrFI RCCGGY 1 cut(s) 39
BsrI ACTGG 2 cut(s) 209, 276
BssAI RCCGGY 1 cut(s) 39
BssMI GATC 6 cut(s) 57, 65, 221, 238, 339, 438
Bst4CI ACNGT 4 cut(s) 104, 146, 374, 517
BstC8I GCNNGC 1 cut(s) 479
BstDEI CTNAG 2 cut(s) 29, 343
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 6 cut(s) 60, 68, 224, 241, 342, 441
BstMAI GTCTC 1 cut(s) 226
BstMBI GATC 6 cut(s) 57, 65, 221, 238, 339, 438
BstMCI CGRYCG 1 cut(s) 441
BstSCI CCNGG 1 cut(s) 347
BstV2I GAAGAC 1 cut(s) 450
BstX2I RGATCY 4 cut(s) 65, 221, 238, 339
BstYI RGATCY 4 cut(s) 65, 221, 238, 339
BsuI GTATCC 1 cut(s) 163
BsuRI GGCC 1 cut(s) 198
BtsIMutI CAGTG 3 cut(s) 269, 379, 513
Cac8I GCNNGC 1 cut(s) 479
CfoI GCGC 1 cut(s) 277
Cfr10I RCCGGY 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 304
CseI GACGC 1 cut(s) 322
Csp6I GTAC 1 cut(s) 281
CspAI ACCGGT 1 cut(s) 39
CviAII CATG 3 cut(s) 200, 428, 527
CviJI RGCY 7 cut(s) 198, 210, 347, 355, 406, 481, 502
CviKI_1 RGCY 7 cut(s) 198, 210, 347, 355, 406, 481, 502
CviQI GTAC 1 cut(s) 281
DdeI CTNAG 2 cut(s) 29, 343
DpnI GATC 6 cut(s) 59, 67, 223, 240, 341, 440
DpnII GATC 6 cut(s) 57, 65, 221, 238, 339, 438
Eco31I GGTCTC 1 cut(s) 226
Eco32I GATATC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 304
EcoRI GAATTC 1 cut(s) 249
EcoRV GATATC 1 cut(s) 35
FaeI CATG 3 cut(s) 203, 431, 530
FaiI YATR 6 cut(s) 201, 294, 296, 429, 473, 528
FatI CATG 3 cut(s) 199, 427, 526
FauNDI CATATG 1 cut(s) 294
GlaI GCGC 1 cut(s) 276
GsuI CTGGAG 1 cut(s) 226
HaeII RGCGCY 1 cut(s) 278
HaeIII GGCC 1 cut(s) 198
HapII CCGG 3 cut(s) 40, 48, 348
HgaI GACGC 1 cut(s) 322
HhaI GCGC 1 cut(s) 277
Hin1II CATG 3 cut(s) 203, 431, 530
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HinfI GANTC 2 cut(s) 120, 164
HpaII CCGG 3 cut(s) 40, 48, 348
HphI GGTGA 4 cut(s) 29, 329, 454, 557
Hpy188I TCNGA 2 cut(s) 169, 216
Hpy188III TCNNGA 3 cut(s) 124, 229, 337
Hpy99I CGWCG 3 cut(s) 216, 314, 338
HpyAV CCTTC 1 cut(s) 542
HpyCH4III ACNGT 4 cut(s) 104, 146, 374, 517
HpyCH4IV ACGT 2 cut(s) 10, 15
HpyCH4V TGCA 1 cut(s) 524
HpyF3I CTNAG 2 cut(s) 29, 343
HpySE526I ACGT 2 cut(s) 10, 15
Hsp92II CATG 3 cut(s) 203, 431, 530
HspAI GCGC 1 cut(s) 275
Kzo9I GATC 6 cut(s) 57, 65, 221, 238, 339, 438
LmnI GCTCC 1 cut(s) 207
MaeII ACGT 2 cut(s) 10, 15
MaeIII GTNAC 4 cut(s) 11, 113, 460, 517
MalI GATC 6 cut(s) 59, 67, 223, 240, 341, 440
MboI GATC 6 cut(s) 57, 65, 221, 238, 339, 438
MboII GAAGA 1 cut(s) 455
MflI RGATCY 4 cut(s) 65, 221, 238, 339
MluCI AATT 3 cut(s) 93, 128, 249
MlyI GAGTC 1 cut(s) 129
MmeI TCCRAC 2 cut(s) 93, 194
MnlI CCTC 6 cut(s) 188, 317, 388, 392, 551, 554
MseI TTAA 1 cut(s) 300
MspI CCGG 3 cut(s) 40, 48, 348
MspR9I CCNGG 1 cut(s) 349
NciI CCSGG 1 cut(s) 349
NdeI CATATG 1 cut(s) 294
NdeII GATC 6 cut(s) 57, 65, 221, 238, 339, 438
NlaIII CATG 3 cut(s) 203, 431, 530
NlaIV GGNNCC 3 cut(s) 137, 209, 223
NmuCI GTSAC 2 cut(s) 113, 460
PfeI GAWTC 1 cut(s) 164
PflFI GACNNNGTC 1 cut(s) 119
PflMI CCANNNNNTGG 1 cut(s) 295
PinAI ACCGGT 1 cut(s) 39
Ple19I CGATCG 1 cut(s) 441
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
PpuMI RGGWCCY 1 cut(s) 304
Psp1406I AACGTT 1 cut(s) 10
Psp5II RGGWCCY 1 cut(s) 304
PspN4I GGNNCC 3 cut(s) 137, 209, 223
PspPI GGNCC 1 cut(s) 304
PspPPI RGGWCCY 1 cut(s) 304
PsuI RGATCY 4 cut(s) 65, 221, 238, 339
PsyI GACNNNGTC 1 cut(s) 119
PvuI CGATCG 1 cut(s) 441
RsaI GTAC 1 cut(s) 282
RsaNI GTAC 1 cut(s) 281
SaqAI TTAA 1 cut(s) 300
Sau3AI GATC 6 cut(s) 57, 65, 221, 238, 339, 438
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 349
SetI ASST 8 cut(s) 13, 18, 306, 357, 408, 483, 547, 553
SinI GGWCC 1 cut(s) 304
Sse9I AATT 3 cut(s) 93, 128, 249
SsiI CCGC 2 cut(s) 420, 465
StyD4I CCNGG 1 cut(s) 347
TaaI ACNGT 4 cut(s) 104, 146, 374, 517
TaiI ACGT 2 cut(s) 13, 18
TaqI TCGA 3 cut(s) 162, 336, 441
TaqII GACCGA 1 cut(s) 132
TasI AATT 3 cut(s) 93, 128, 249
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 3 cut(s) 276, 379, 520
TseFI GTSAC 2 cut(s) 113, 460
Tsp45I GTSAC 2 cut(s) 113, 460
TspDTI ATGAA 3 cut(s) 279, 429, 543
TspRI CASTG 3 cut(s) 276, 379, 520
Tth111I GACNNNGTC 1 cut(s) 119
Van91I CCANNNNNTGG 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 304
XapI RAATTY 2 cut(s) 128, 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.