Rh1BG174800
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0014796
Physical Location & Seq
Reverse (-)
27832543 .. 27836983
4441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG174800.1

Sequence Viewer

Length: 972 bp
ATGGTCCTCCATACTGGCCACCGGATCCAGCATCTCAGCCGGAGTGGCTCCACTCCACCTTCTTATTTCTGTCTCAAGACCCAAATTCCAAGCCTCAACGAAGCGATCCCTATCGGAAGACCTGTTAGACCGAAAACTGTCGCTGTGATTGACCGGGAAGGGAAACAGCCTCATCCTGGCGGGCCTAGAGGAGGGCCGGTAGAGGCGATCGTACTCGAGGTAGTCGTCGAGGTCTGGCATCATGTGCTCGAGGTCGTCGTCGTTGGTGACGGAGATCAAGGCGTCGAGGTCTTCCCCGGGGAGCTGGTACTTGAACTACACCTCGCTGTGGGAGAGGGCGGAGAGCTTGATGGTGCGTTCGACGGCGAGGATCTTGGTCTCCCCGCCGGTGTAGAAGAAGTAATGGAAGCTGGTGATCATCAAGAGGGAGCAGAGAGCTCATCATCCTCATCATCAAAAACTAGTGGCAGTTCTAGAACTTCGTCGTCGTCGTCATCATCATCATCATCATTCACTAGCGCACTGACTCTAACCCAATTGGGGGTTGCTCATGTGCCTCAACGCTATGTTCTCCCTCCTTTCCAACGACCAAGTCCTTATCCAAACCTCTCTACTATTGCTCTTCCCATACTTGACCTTTCCTCTTTGCAAAGTTCTTCTCTTAGGCCTCATGTGATCAACAATATTCAGACTGCTTGCAGGGAAATAGGGTTCTTTCAGGTTACCAACCATGGAATTCCTCTTTCGATGATGAAAGATGCCCTCAGTGCTGCAAATGAGTTCTTTAACCTACCTGTTGAGGAAAAGATGCTTCTAGGGTCTGACAATGTTCATGCACCTGTAAGATATGGGACAAGCATAAATCAAGCTGTTGATAGGGTTCACTTTTGGAGAGACTTCATCAAGCACTACTCCCATCCTATCTCAAAATGGATCCATCTTTGGCCATCAAACCCTTCTTCTTACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

323

Amino Acids

34.94

Weight (kDa)

5.86

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 208 - 318 8.4e-27 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 180, 339, 384
AclWI GGATC 6 cut(s) 19, 32, 100, 378, 928, 941
AcoI YGGCCR 2 cut(s) 16, 944
AcsI RAATTY 2 cut(s) 84, 735
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 2 cut(s) 213, 309
AfiI CCNNNNNNNGG 5 cut(s) 176, 191, 328, 540, 541
AgsI TTSAA 1 cut(s) 314
AhlI ACTAGT 1 cut(s) 461
AjnI CCWGG 1 cut(s) 175
AloI GAACNNNNNNTCC 2 cut(s) 695, 727
AluBI AGCT 5 cut(s) 304, 346, 410, 438, 869
AluI AGCT 5 cut(s) 304, 346, 410, 438, 869
Alw21I GWGCWC 2 cut(s) 249, 440
Alw26I GTCTC 3 cut(s) 77, 383, 888
AlwI GGATC 6 cut(s) 19, 32, 100, 378, 928, 941
Ama87I CYCGRG 3 cut(s) 215, 248, 296
AoxI GGCC 5 cut(s) 16, 182, 194, 665, 944
ApeKI GCWGC 1 cut(s) 770
ApoI RAATTY 2 cut(s) 84, 735
AspLEI GCGC 1 cut(s) 521
AspS9I GGNCC 3 cut(s) 4, 182, 194
AsuC2I CCSGG 3 cut(s) 155, 297, 298
AsuHPI GGTGA 2 cut(s) 278, 425
AvaI CYCGRG 3 cut(s) 215, 248, 296
AvaII GGWCC 1 cut(s) 4
BalI TGGCCA 2 cut(s) 18, 946
BamHI GGATCC 2 cut(s) 24, 933
BanII GRGCYC 1 cut(s) 440
BbsI GAAGAC 2 cut(s) 124, 283
Bbv12I GWGCWC 2 cut(s) 249, 440
BbvI GCAGC 1 cut(s) 757
BccI CCATC 4 cut(s) 344, 924, 945, 955
BceAI ACGGC 1 cut(s) 379
BciT130I CCWGG 1 cut(s) 177
BclI TGATCA 2 cut(s) 415, 675
BcnI CCSGG 3 cut(s) 155, 297, 298
BcoDI GTCTC 3 cut(s) 77, 383, 888
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 5 cut(s) 186, 462, 474, 516, 815
BglI GCCNNNNNGGC 1 cut(s) 45
BisI GCNGC 1 cut(s) 771
BlsI GCNGC 1 cut(s) 772
Bme1390I CCNGG 4 cut(s) 155, 177, 297, 298
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 3 cut(s) 215, 248, 296
BmgT120I GGNCC 3 cut(s) 4, 182, 194
BmiI GGNNCC 3 cut(s) 26, 49, 935
BmrFI CCNGG 4 cut(s) 155, 177, 297, 298
BmsI GCATC 4 cut(s) 40, 247, 748, 798
BpiI GAAGAC 2 cut(s) 124, 283
BpuEI CTTGAG 1 cut(s) 59
BpuMI CCSGG 3 cut(s) 155, 297, 298
BsaBI GATNNNNATC 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 4 cut(s) 295, 296, 297, 730
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 191, 328, 540, 541
Bse118I RCCGGY 2 cut(s) 196, 386
Bse1I ACTGG 1 cut(s) 19
Bse8I GATNNNNATC 1 cut(s) 110
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 4 cut(s) 295, 296, 297, 730
BseGI GGATG 3 cut(s) 172, 443, 916
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 5 cut(s) 176, 191, 328, 540, 541
BseMII CTCAG 2 cut(s) 49, 778
BseNI ACTGG 1 cut(s) 19
BseRI GAGGAG 1 cut(s) 204
BseXI GCAGC 1 cut(s) 757
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 5 cut(s) 18, 184, 196, 667, 946
BsiEI CGRYCG 1 cut(s) 210
BsiHKAI GWGCWC 2 cut(s) 249, 440
BsiHKCI CYCGRG 3 cut(s) 215, 248, 296
BsiSI CCGG 6 cut(s) 22, 40, 154, 197, 297, 387
BslFI GGGAC 1 cut(s) 865
BslI CCNNNNNNNGG 5 cut(s) 176, 191, 328, 540, 541
BsmAI GTCTC 3 cut(s) 77, 383, 888
BsmFI GGGAC 1 cut(s) 865
BsnI GGCC 5 cut(s) 18, 184, 196, 667, 946
Bso31I GGTCTC 1 cut(s) 383
BsoBI CYCGRG 3 cut(s) 215, 248, 296
Bsp1286I GDGCHC 2 cut(s) 249, 440
Bsp143I GATC 8 cut(s) 24, 105, 207, 274, 370, 415, 675, 933
Bsp19I CCATGG 1 cut(s) 730
BspACI CCGC 3 cut(s) 180, 339, 384
BspANI GGCC 5 cut(s) 18, 184, 196, 667, 946
BspCNI CTCAG 2 cut(s) 48, 777
BspLI GGNNCC 3 cut(s) 26, 49, 935
BspPI GGATC 6 cut(s) 19, 32, 100, 378, 928, 941
BspQI GCTCTTC 1 cut(s) 627
BspTNI GGTCTC 1 cut(s) 383
BsrFI RCCGGY 2 cut(s) 196, 386
BsrI ACTGG 1 cut(s) 19
BssAI RCCGGY 2 cut(s) 196, 386
BssECI CCNNGG 4 cut(s) 295, 296, 297, 730
BssMI GATC 8 cut(s) 24, 105, 207, 274, 370, 415, 675, 933
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 730
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 627
BstACI GRCGYC 1 cut(s) 282
BstAPI GCANNNNNTGC 1 cut(s) 244
BstC8I GCNNGC 2 cut(s) 182, 697
BstDEI CTNAG 3 cut(s) 35, 662, 764
BstDSI CCRYGG 1 cut(s) 730
BstEII GGTNACC 1 cut(s) 721
BstENI CCTNNNNNAGG 1 cut(s) 189
BstF5I GGATG 3 cut(s) 172, 443, 916
BstHHI GCGC 1 cut(s) 521
BstKTI GATC 8 cut(s) 27, 108, 210, 277, 373, 418, 678, 936
BstMAI GTCTC 3 cut(s) 77, 383, 888
BstMBI GATC 8 cut(s) 24, 105, 207, 274, 370, 415, 675, 933
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 3 cut(s) 45, 244, 767
BstNI CCWGG 1 cut(s) 177
BstPI GGTNACC 1 cut(s) 721
BstSCI CCNGG 4 cut(s) 153, 175, 295, 296
BstV1I GCAGC 1 cut(s) 757
BstV2I GAAGAC 2 cut(s) 124, 283
BstX2I RGATCY 3 cut(s) 24, 370, 933
BstYI RGATCY 3 cut(s) 24, 370, 933
BsuRI GGCC 5 cut(s) 18, 184, 196, 667, 946
BtgI CCRYGG 1 cut(s) 730
BtsCI GGATG 3 cut(s) 172, 443, 916
BtsIMutI CAGTG 2 cut(s) 521, 772
Cac8I GCNNGC 2 cut(s) 182, 697
CfoI GCGC 1 cut(s) 521
Cfr10I RCCGGY 2 cut(s) 196, 386
Cfr13I GGNCC 3 cut(s) 4, 182, 194
Cfr9I CCCGGG 1 cut(s) 296
CseI GACGC 1 cut(s) 271
Csp6I GTAC 2 cut(s) 212, 308
CviAII CATG 5 cut(s) 242, 551, 671, 731, 833
CviQI GTAC 2 cut(s) 212, 308
DdeI CTNAG 3 cut(s) 35, 662, 764
DpnI GATC 8 cut(s) 26, 107, 209, 276, 372, 417, 677, 935
DpnII GATC 8 cut(s) 24, 105, 207, 274, 370, 415, 675, 933
EaeI YGGCCR 2 cut(s) 16, 944
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
EciI GGCGGA 1 cut(s) 354
Ecl136II GAGCTC 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 730
Eco147I AGGCCT 1 cut(s) 667
Eco24I GRGCYC 1 cut(s) 440
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 1 cut(s) 438
Eco88I CYCGRG 3 cut(s) 215, 248, 296
Eco91I GGTNACC 1 cut(s) 721
EcoICRI GAGCTC 1 cut(s) 438
EcoNI CCTNNNNNAGG 1 cut(s) 189
EcoO65I GGTNACC 1 cut(s) 721
EcoRI GAATTC 1 cut(s) 735
EcoRII CCWGG 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 730
EcoT38I GRGCYC 1 cut(s) 440
ErhI CCWWGG 1 cut(s) 730
FaeI CATG 5 cut(s) 245, 554, 674, 734, 836
FaqI GGGAC 1 cut(s) 865
FatI CATG 5 cut(s) 241, 550, 670, 730, 832
FauI CCCGC 2 cut(s) 173, 391
FbaI TGATCA 2 cut(s) 415, 675
Fnu4HI GCNGC 1 cut(s) 771
FokI GGATG 3 cut(s) 159, 430, 903
FriOI GRGCYC 1 cut(s) 440
Fsp4HI GCNGC 1 cut(s) 771
FspBI CTAG 5 cut(s) 186, 462, 474, 516, 815
GlaI GCGC 1 cut(s) 520
GluI GCNGC 1 cut(s) 771
HaeIII GGCC 5 cut(s) 18, 184, 196, 667, 946
HapII CCGG 6 cut(s) 22, 40, 154, 197, 297, 387
HgaI GACGC 1 cut(s) 271
HhaI GCGC 1 cut(s) 521
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 5 cut(s) 245, 554, 674, 734, 836
Hin6I GCGC 1 cut(s) 519
HinP1I GCGC 1 cut(s) 519
HinfI GANTC 1 cut(s) 526
HpaII CCGG 6 cut(s) 22, 40, 154, 197, 297, 387
HphI GGTGA 2 cut(s) 278, 425
Hpy166II GTNNAC 1 cut(s) 883
Hpy188I TCNGA 3 cut(s) 116, 690, 823
Hpy188III TCNNGA 3 cut(s) 76, 422, 474
Hpy8I GTNNAC 1 cut(s) 883
Hpy99I CGWCG 8 cut(s) 230, 260, 263, 287, 365, 487, 490, 493
HpyAV CCTTC 3 cut(s) 69, 152, 966
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 4 cut(s) 649, 699, 773, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 244, 767
HpyF3I CTNAG 3 cut(s) 35, 662, 764
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 5 cut(s) 245, 554, 674, 734, 836
HspAI GCGC 1 cut(s) 519
Ksp22I TGATCA 2 cut(s) 415, 675
Kzo9I GATC 8 cut(s) 24, 105, 207, 274, 370, 415, 675, 933
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 3 cut(s) 53, 301, 428
Lsp1109I GCAGC 1 cut(s) 757
LweI GCATC 4 cut(s) 40, 247, 748, 798
MaeI CTAG 5 cut(s) 186, 462, 474, 516, 815
MaeIII GTNAC 2 cut(s) 266, 721
MalI GATC 8 cut(s) 26, 107, 209, 276, 372, 417, 677, 935
MboI GATC 8 cut(s) 24, 105, 207, 274, 370, 415, 675, 933
MboII GAAGA 6 cut(s) 129, 283, 407, 614, 648, 951
MfeI CAATTG 1 cut(s) 536
MflI RGATCY 3 cut(s) 24, 370, 933
MhlI GDGCHC 2 cut(s) 249, 440
MlsI TGGCCA 2 cut(s) 18, 946
MluCI AATT 3 cut(s) 84, 536, 735
MluNI TGGCCA 2 cut(s) 18, 946
MlyI GAGTC 1 cut(s) 520
MmeI TCCRAC 1 cut(s) 607
Mox20I TGGCCA 2 cut(s) 18, 946
MscI TGGCCA 2 cut(s) 18, 946
MseI TTAA 1 cut(s) 786
Msp20I TGGCCA 2 cut(s) 18, 946
MspI CCGG 6 cut(s) 22, 40, 154, 197, 297, 387
MspR9I CCNGG 4 cut(s) 155, 177, 297, 298
MunI CAATTG 1 cut(s) 536
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 3 cut(s) 45, 244, 767
NciI CCSGG 3 cut(s) 155, 297, 298
NcoI CCATGG 1 cut(s) 730
NdeII GATC 8 cut(s) 24, 105, 207, 274, 370, 415, 675, 933
NlaIII CATG 5 cut(s) 245, 554, 674, 734, 836
NlaIV GGNNCC 3 cut(s) 26, 49, 935
NmuCI GTSAC 1 cut(s) 266
PaeR7I CTCGAG 2 cut(s) 215, 248
PceI AGGCCT 1 cut(s) 667
PciSI GCTCTTC 1 cut(s) 627
PcsI WCGNNNNNNNCGW 3 cut(s) 222, 255, 488
PflFI GACNNNGTC 1 cut(s) 591
PkrI GCNGC 1 cut(s) 772
Ple19I CGATCG 1 cut(s) 210
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
Psp124BI GAGCTC 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 175
PspEI GGTNACC 1 cut(s) 721
PspGI CCWGG 1 cut(s) 175
PspN4I GGNNCC 3 cut(s) 26, 49, 935
PspPI GGNCC 3 cut(s) 4, 182, 194
PspXI VCTCGAGB 2 cut(s) 215, 248
PsuI RGATCY 3 cut(s) 24, 370, 933
PsyI GACNNNGTC 1 cut(s) 591
PvuI CGATCG 1 cut(s) 210
RsaI GTAC 2 cut(s) 213, 309
RsaNI GTAC 2 cut(s) 212, 308
SacI GAGCTC 1 cut(s) 440
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 1 cut(s) 786
SatI GCNGC 1 cut(s) 771
Sau3AI GATC 8 cut(s) 24, 105, 207, 274, 370, 415, 675, 933
Sau96I GGNCC 3 cut(s) 4, 182, 194
SchI GAGTC 1 cut(s) 520
ScrFI CCNGG 4 cut(s) 155, 177, 297, 298
SduI GDGCHC 2 cut(s) 249, 440
SfaNI GCATC 4 cut(s) 40, 247, 748, 798
Sfr274I CTCGAG 2 cut(s) 215, 248
SgrAI CRCCGGYG 1 cut(s) 386
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 2 cut(s) 215, 248
SmaI CCCGGG 1 cut(s) 298
SmlI CTYRAG 3 cut(s) 74, 215, 248
SmoI CTYRAG 3 cut(s) 74, 215, 248
SpeI ACTAGT 1 cut(s) 461
Sse9I AATT 3 cut(s) 84, 536, 735
SseBI AGGCCT 1 cut(s) 667
SsiI CCGC 3 cut(s) 180, 339, 384
SspI AATATT 1 cut(s) 685
SspMI CTAG 5 cut(s) 186, 462, 474, 516, 815
SstI GAGCTC 1 cut(s) 440
StuI AGGCCT 1 cut(s) 667
StyD4I CCNGG 4 cut(s) 153, 175, 295, 296
StyI CCWWGG 1 cut(s) 730
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 6 cut(s) 216, 228, 249, 285, 360, 746
TaqII GACCGA 1 cut(s) 145
TasI AATT 3 cut(s) 84, 536, 735
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TscAI CASTG 2 cut(s) 528, 772
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 1 cut(s) 770
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 3 cut(s) 767, 821, 889
TspGWI ACGGA 1 cut(s) 285
TspMI CCCGGG 1 cut(s) 296
TspRI CASTG 2 cut(s) 528, 772
Tth111I GACNNNGTC 1 cut(s) 591
VpaK11BI GGWCC 1 cut(s) 4
XagI CCTNNNNNAGG 1 cut(s) 189
XapI RAATTY 2 cut(s) 84, 735
XbaI TCTAGA 1 cut(s) 473
XhoI CTCGAG 2 cut(s) 215, 248
XmaI CCCGGG 1 cut(s) 296
XspI CTAG 5 cut(s) 186, 462, 474, 516, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.