RLG00000032972

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014796
Physical Location & Seq
Reverse (-)
21568429 .. 21569874
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032972

Sequence Viewer

Length: 693 bp
ATGTTGGTTGTGGAAAATGATGACCTGGTTTATAGTACTGTGATGCTCATGCTCATACATGTAATCTCTTGTCTACCACAAAATCGCATTGATGCTCTTGAAAGAATTCAAAATCACATTGATCCACCATACTGGCCACCAGATCCAGCATCTCAGCCGGGGAGGTTCCACTCCACCTTATTTCTTTCTCAGGATCCAAAAAACAAAAAAAACAAAAAGAAGACAGCCTGTGTTGGGCTCGATGTCGTAGTCCTCTATACTGGCCACCAGATCCAGCATCTCAGTCGGAGAGGCTTCACTCCACCTTCTTATTTCTGTCTTAGGACCCAAATTCAAAGCCTCAACGAAGAGATCCCTATCGGACGACCTGTTAGACCCGAAACTGTAGCTGTGATTGATCGGTTAGAGAAACAGCCTCATCGTGGCGGAGGCCCGGTAGAGGCGATTGTACTCGTGCATCATGTGCTCGAGGTCGTCGACATTGGTGACGAAGATCAAGGCGTTGAGGTCTTCCCCGGGGAGCTGGCACTTGAACGACATGTCGCTGTCGGAGAGGGCGGAGAGCCTGACAACAAGGGCGGGGAATTTGATGGTGCGTTCAATGGTGAGGATCTTGGTCTCGCCGCCGGTGTAGGTGAGCTGGTTGTCGTGGGGGCGGGGGCGTGTGGTTATGGTGGTTTTAGATCTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

231

Amino Acids

24.99

Weight (kDa)

5.22

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 73, 477
AciI CCGC 5 cut(s) 426, 558, 579, 624, 656
AclWI GGATC 7 cut(s) 116, 137, 188, 201, 265, 346, 618
AcoI YGGCCR 2 cut(s) 134, 262
AcsI RAATTY 3 cut(s) 105, 330, 584
AfaI GTAC 2 cut(s) 37, 450
AfiI CCNNNNNNNGG 3 cut(s) 234, 422, 439
AflIII ACRYGT 2 cut(s) 58, 538
AgsI TTSAA 5 cut(s) 101, 110, 335, 533, 601
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 3 cut(s) 389, 523, 640
AluI AGCT 3 cut(s) 389, 523, 640
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 1 cut(s) 623
AlwI GGATC 7 cut(s) 116, 137, 188, 201, 265, 346, 618
Ama87I CYCGRG 2 cut(s) 467, 515
AoxI GGCC 3 cut(s) 134, 262, 430
ApoI RAATTY 3 cut(s) 105, 330, 584
Asp700I GAANNNNTTC 1 cut(s) 105
AspS9I GGNCC 2 cut(s) 324, 431
AsuC2I CCSGG 4 cut(s) 159, 434, 516, 517
AsuHPI GGTGA 3 cut(s) 497, 617, 647
AvaI CYCGRG 2 cut(s) 467, 515
AvaII GGWCC 1 cut(s) 324
BalI TGGCCA 2 cut(s) 136, 264
BamHI GGATCC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 240
BauI CACGAG 1 cut(s) 452
BbsI GAAGAC 2 cut(s) 227, 502
Bbv12I GWGCWC 1 cut(s) 468
BccI CCATC 1 cut(s) 584
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciT130I CCWGG 1 cut(s) 26
BcnI CCSGG 4 cut(s) 159, 434, 516, 517
BcoDI GTCTC 1 cut(s) 623
BfmI CTRYAG 1 cut(s) 384
BglII AGATCT 1 cut(s) 683
BisI GCNGC 1 cut(s) 624
BlsI GCNGC 1 cut(s) 625
BmcAI AGTACT 1 cut(s) 37
Bme1390I CCNGG 5 cut(s) 26, 159, 434, 516, 517
Bme18I GGWCC 1 cut(s) 324
BmeT110I CYCGRG 2 cut(s) 467, 515
BmgT120I GGNCC 2 cut(s) 324, 431
BmiI GGNNCC 3 cut(s) 167, 195, 326
BmrFI CCNGG 5 cut(s) 26, 159, 434, 516, 517
BmsI GCATC 5 cut(s) 33, 82, 158, 286, 466
BpiI GAAGAC 2 cut(s) 227, 502
BpuMI CCSGG 4 cut(s) 159, 434, 516, 517
BsaBI GATNNNNATC 1 cut(s) 356
BsaI GGTCTC 1 cut(s) 623
BsaJI CCNNGG 4 cut(s) 158, 514, 515, 516
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 3 cut(s) 234, 422, 439
Bse118I RCCGGY 1 cut(s) 626
Bse1I ACTGG 2 cut(s) 137, 265
Bse8I GATNNNNATC 1 cut(s) 356
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 4 cut(s) 158, 514, 515, 516
BseJI GATNNNNATC 1 cut(s) 356
BseLI CCNNNNNNNGG 3 cut(s) 234, 422, 439
BseMII CTCAG 3 cut(s) 167, 203, 295
BseNI ACTGG 2 cut(s) 137, 265
BshFI GGCC 3 cut(s) 136, 264, 432
BsiHKAI GWGCWC 1 cut(s) 468
BsiHKCI CYCGRG 2 cut(s) 467, 515
BsiSI CCGG 4 cut(s) 158, 434, 516, 627
BslI CCNNNNNNNGG 3 cut(s) 234, 422, 439
BsmAI GTCTC 1 cut(s) 623
BsnI GGCC 3 cut(s) 136, 264, 432
Bso31I GGTCTC 1 cut(s) 623
BsoBI CYCGRG 2 cut(s) 467, 515
Bsp1286I GDGCHC 2 cut(s) 240, 468
Bsp143I GATC 9 cut(s) 121, 142, 193, 270, 351, 397, 493, 610, 683
BspACI CCGC 5 cut(s) 426, 558, 579, 624, 656
BspANI GGCC 3 cut(s) 136, 264, 432
BspCNI CTCAG 3 cut(s) 166, 202, 294
BspLI GGNNCC 3 cut(s) 167, 195, 326
BspPI GGATC 7 cut(s) 116, 137, 188, 201, 265, 346, 618
BspTNI GGTCTC 1 cut(s) 623
BsrFI RCCGGY 1 cut(s) 626
BsrI ACTGG 2 cut(s) 137, 265
BssAI RCCGGY 1 cut(s) 626
BssECI CCNNGG 4 cut(s) 158, 514, 515, 516
BssMI GATC 9 cut(s) 121, 142, 193, 270, 351, 397, 493, 610, 683
BssSI CACGAG 1 cut(s) 452
Bst2BI CACGAG 1 cut(s) 452
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 2 cut(s) 40, 385
Bst6I CTCTTC 1 cut(s) 342
BstAPI GCANNNNNTGC 1 cut(s) 463
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 4 cut(s) 153, 189, 281, 320
BstKTI GATC 9 cut(s) 124, 145, 196, 273, 354, 400, 496, 613, 686
BstMAI GTCTC 1 cut(s) 623
BstMBI GATC 9 cut(s) 121, 142, 193, 270, 351, 397, 493, 610, 683
BstMWI GCNNNNNNNGC 1 cut(s) 463
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 2 cut(s) 62, 542
BstSCI CCNGG 5 cut(s) 24, 157, 432, 514, 515
BstSFI CTRYAG 1 cut(s) 384
BstV2I GAAGAC 2 cut(s) 227, 502
BstX2I RGATCY 6 cut(s) 142, 193, 270, 351, 610, 683
BstXI CCANNNNNNTGG 1 cut(s) 132
BstYI RGATCY 6 cut(s) 142, 193, 270, 351, 610, 683
BsuRI GGCC 3 cut(s) 136, 264, 432
Cac8I GCNNGC 1 cut(s) 525
Cfr10I RCCGGY 1 cut(s) 626
Cfr13I GGNCC 2 cut(s) 324, 431
Cfr9I CCCGGG 1 cut(s) 515
CsiI ACCWGGT 1 cut(s) 24
Csp6I GTAC 2 cut(s) 36, 449
CviAII CATG 4 cut(s) 49, 59, 461, 539
CviQI GTAC 2 cut(s) 36, 449
DdeI CTNAG 4 cut(s) 153, 189, 281, 320
DpnI GATC 9 cut(s) 123, 144, 195, 272, 353, 399, 495, 612, 685
DpnII GATC 9 cut(s) 121, 142, 193, 270, 351, 397, 493, 610, 683
EaeI YGGCCR 2 cut(s) 134, 262
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
EciI GGCGGA 2 cut(s) 441, 573
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 324
Eco88I CYCGRG 2 cut(s) 467, 515
EcoO109I RGGNCCY 1 cut(s) 324
EcoRI GAATTC 1 cut(s) 105
EcoRII CCWGG 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 240
FaeI CATG 4 cut(s) 52, 62, 464, 542
FaiI YATR 9 cut(s) 33, 50, 56, 60, 130, 258, 462, 540, 672
FatI CATG 4 cut(s) 48, 58, 460, 538
FauI CCCGC 2 cut(s) 572, 649
FblI GTMKAC 2 cut(s) 73, 477
Fnu4HI GCNGC 1 cut(s) 624
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 624
GluI GCNGC 1 cut(s) 624
HaeIII GGCC 3 cut(s) 136, 264, 432
HapII CCGG 4 cut(s) 158, 434, 516, 627
Hin1II CATG 4 cut(s) 52, 62, 464, 542
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HpaII CCGG 4 cut(s) 158, 434, 516, 627
HphI GGTGA 3 cut(s) 497, 617, 647
Hpy166II GTNNAC 2 cut(s) 74, 478
Hpy188I TCNGA 3 cut(s) 288, 362, 551
Hpy188III TCNNGA 3 cut(s) 98, 191, 687
Hpy8I GTNNAC 2 cut(s) 74, 478
Hpy99I CGWCG 1 cut(s) 479
HpyAV CCTTC 1 cut(s) 315
HpyCH4III ACNGT 2 cut(s) 40, 385
HpyCH4V TGCA 1 cut(s) 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 463
HpyF3I CTNAG 4 cut(s) 153, 189, 281, 320
Hsp92II CATG 4 cut(s) 52, 62, 464, 542
Kzo9I GATC 9 cut(s) 121, 142, 193, 270, 351, 397, 493, 610, 683
LmnI GCTCC 1 cut(s) 520
LweI GCATC 5 cut(s) 33, 82, 158, 286, 466
MabI ACCWGGT 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 485
MalI GATC 9 cut(s) 123, 144, 195, 272, 353, 399, 495, 612, 685
MboI GATC 9 cut(s) 121, 142, 193, 270, 351, 397, 493, 610, 683
MboII GAAGA 4 cut(s) 232, 359, 502, 503
MflI RGATCY 6 cut(s) 142, 193, 270, 351, 610, 683
MhlI GDGCHC 2 cut(s) 240, 468
MlsI TGGCCA 2 cut(s) 136, 264
MluCI AATT 3 cut(s) 105, 330, 584
MluNI TGGCCA 2 cut(s) 136, 264
MmeI TCCRAC 2 cut(s) 266, 529
Mox20I TGGCCA 2 cut(s) 136, 264
MroXI GAANNNNTTC 1 cut(s) 105
MscI TGGCCA 2 cut(s) 136, 264
Msp20I TGGCCA 2 cut(s) 136, 264
MspI CCGG 4 cut(s) 158, 434, 516, 627
MspR9I CCNGG 5 cut(s) 26, 159, 434, 516, 517
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 1 cut(s) 463
NciI CCSGG 4 cut(s) 159, 434, 516, 517
NdeII GATC 9 cut(s) 121, 142, 193, 270, 351, 397, 493, 610, 683
NlaIII CATG 4 cut(s) 52, 62, 464, 542
NlaIV GGNNCC 3 cut(s) 167, 195, 326
NmuCI GTSAC 1 cut(s) 485
NspI RCATGY 2 cut(s) 62, 542
PaeR7I CTCGAG 1 cut(s) 467
PciI ACATGT 2 cut(s) 58, 538
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PdmI GAANNNNTTC 1 cut(s) 105
PkrI GCNGC 1 cut(s) 625
PpuMI RGGWCCY 1 cut(s) 324
PscI ACATGT 2 cut(s) 58, 538
Psp5II RGGWCCY 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 3 cut(s) 167, 195, 326
PspPI GGNCC 2 cut(s) 324, 431
PspPPI RGGWCCY 1 cut(s) 324
PspXI VCTCGAGB 1 cut(s) 467
PsuI RGATCY 6 cut(s) 142, 193, 270, 351, 610, 683
RsaI GTAC 2 cut(s) 37, 450
RsaNI GTAC 2 cut(s) 36, 449
SalI GTCGAC 1 cut(s) 476
SatI GCNGC 1 cut(s) 624
Sau3AI GATC 9 cut(s) 121, 142, 193, 270, 351, 397, 493, 610, 683
Sau96I GGNCC 2 cut(s) 324, 431
ScaI AGTACT 1 cut(s) 37
ScrFI CCNGG 5 cut(s) 26, 159, 434, 516, 517
SduI GDGCHC 2 cut(s) 240, 468
SexAI ACCWGGT 1 cut(s) 24
SfaNI GCATC 5 cut(s) 33, 82, 158, 286, 466
SfcI CTRYAG 1 cut(s) 384
Sfr274I CTCGAG 1 cut(s) 467
SgrAI CRCCGGYG 1 cut(s) 626
SinI GGWCC 1 cut(s) 324
SlaI CTCGAG 1 cut(s) 467
SmaI CCCGGG 1 cut(s) 517
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
Sse9I AATT 3 cut(s) 105, 330, 584
SsiI CCGC 5 cut(s) 426, 558, 579, 624, 656
StyD4I CCNGG 5 cut(s) 24, 157, 432, 514, 515
TaaI ACNGT 2 cut(s) 40, 385
TaqI TCGA 3 cut(s) 240, 468, 477
TasI AATT 3 cut(s) 105, 330, 584
TatI WGTACW 2 cut(s) 35, 448
TauI GCSGC 1 cut(s) 626
TseFI GTSAC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 485
TspMI CCCGGG 1 cut(s) 515
VpaK11BI GGWCC 1 cut(s) 324
XapI RAATTY 3 cut(s) 105, 330, 584
XceI RCATGY 2 cut(s) 62, 542
XhoI CTCGAG 1 cut(s) 467
XmaI CCCGGG 1 cut(s) 515
XmiI GTMKAC 2 cut(s) 73, 477
XmnI GAANNNNTTC 1 cut(s) 105
ZrmI AGTACT 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.