FvH4_7g13290

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009758
Physical Location & Seq
Forward (+)
11968940 .. 11973028
4089 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g13290.t1

Sequence Viewer

Length: 1884 bp
ATGGCGAAGTTCTCACTGAAGCCAAAGCGGACCAACCTGTACTTTTTAACGCTGACCACAGTGCTCTGCACACTCTTCTACCTAATTGGCGTCTGGCAGCACTCCGCCGGAACCACCTCCTCCGCCGCCACCACCTCCGTCACCTCCGCCATCTCCTGCCCCCACCTCCTCCCCAACATCACAACCCACCTCGACTTCGCCGCCCACCACCGCGCCGAGGACCTCCCCCTCCCTCCCACGACGGCGCGTGTCTCCCACGTGCCGCCCTGCGACGCCAGGCTCGCCGAGTACACCCCCTGCGAGGACGCCACCCGGTCCCTCAAGTTCGAGCGGGACCGGCTGGTTTACCGGGAGAGGCATTGCCCGGAGAAGAACGAGCTCCTCAAGTGCCGGATTCCGGCGCCGCACGGGTACCAAGTGCCGTTCCGGTGGCCGGAGAGCCGGGGGTCGGTGTGGTACGCCAACGTGCCGCATAAGGAGTTGACCGTGGAGAAGAAGAATCAGAATTGGGTTCACTACGAGGGTGACCGGTTTCGATTCCCTGGGGGAGGGACCATGTTCCCTAATGGTGCGGACGCCTATATTGACGATATTGGTAGGTTGATTAATCTCAGAGACGGGTCTATCAGGACCGCCATCGATACCGGTTGTGGGGTTGCAAGTTGGGGTGGTTACCTTTTGTCTAGAGACATTTTAACAGTGTCATTTGCACCAAGAGACACTCATGAAGCACAAGTTCAGTTTGCTCTTGAAAGAGGAATTCCTGCAGTGATTGGGGTAATGGCTTCTATTAGACTTCCCTATCCCTCAAGAGCTTTCGACATGGCTCACTGCTCTCGTTGCCTCATTCCTTGGGGAGAATACGATGGCCTGTACTTGATTGAGGTTGATCGTGTTTTACGACCCGGTGGTTATTGGATTCTTTCCGGCCCACCAATCAACTGGCAGAAGCACTGGAAAGGTTGGGAAAGAACTCAGGCAGACCTCAAAGCTGAGCAGGACAAGATCGAGAGAGTAGCAAAAAGCTTATGCTGGAAAAAATTGAAGCAGAAGGATGACCTTGCCATTTGGCAGAAACCCACTAACCATATCCACTGTAAAGCCAATAGAAAGGTCTTCAAGAAACCATCCTTTTGCCAAGTTCAAAATCCAGACACCGCATGGTATACTAAACTTGAGGAGTGTTTGACTCCACTGCCTGGAGTGAACAACTTAAGAGATACTGCAGGTGGGGAATTGGCTAAATGGCCAGCGAGGCTGACTACAGTTCCTCCAAGAATTAGTAGTGGAAGTTTGGAAGGAATCACAGATGAGATGTTCAGAGAAAACACGGCGCTGTGGAAACAGAGAGTTGCACATTATAAGACCTTGGACTATCAGTTGGCAGACCCTGGGAGGTACCGGAATTTGCTTGACATGAACTCGTACTTGGGAAGCTTTGCAGCTGCACTGGTCGATGATCCTGTGTGGGTTATGAACATTGTCCCGGTTGAGGCTGAGGTCAACACCCTTGGAGCTATCTATGAACGTGGATTGATCGGAACTTATCAGAACTGGTGTGAGGCCATGTCTACTTATCCAAGAACCTATGACTTCATCCACTCTGATTCAGTTTTCACTCTTTACAAGGACAGATGTGAAATGGTAGACATTCTTCTAGAAATGGATAGGATCTTAAGGCCACAAGGCAGCATAATTTTCAGGGATGATGTGGATATGGTTGTGAAGGCCAAGGGGCTCTTGGATGCGATGCAATATGACGCCAGAATAGTAGACCATGAAGAGGGTCCTCATCATAGAGAAAAGATCTTGTTGGCGGCTAAGCAGTACTGGACAGCACCAGAACCAAACCAAAATGAAGGAGAAAGCAGTAAAGAATCATAG

Protein Analysis

628

Amino Acids

71.41

Weight (kDa)

7.28

Isoelectric Point (pI)

32.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 96 - 608 1.4e-223 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1362
AarI CACCTGC 1 cut(s) 1217
Acc36I ACCTGC 1 cut(s) 1217
Acc65I GGTACC 2 cut(s) 411, 1398
AccB1I GGYRCC 3 cut(s) 400, 411, 1398
AccB7I CCANNNNNTGG 1 cut(s) 1199
AccBSI CCGCTC 1 cut(s) 331
AccI GTMKAC 4 cut(s) 1166, 1571, 1647, 1773
AccII CGCG 2 cut(s) 213, 247
AclWI GGATC 2 cut(s) 1454, 1679
AcoI YGGCCR 2 cut(s) 431, 1247
AcsI RAATTY 2 cut(s) 759, 1405
AcuI CTGAAG 1 cut(s) 38
AcvI CACGTG 1 cut(s) 259
AcyI GRCGYC 6 cut(s) 90, 273, 306, 401, 576, 1761
AfaI GTAC 8 cut(s) 41, 290, 413, 458, 875, 1400, 1427, 1829
AflII CTTAAG 2 cut(s) 1213, 1675
AgeI ACCGGT 2 cut(s) 528, 644
AgsI TTSAA 4 cut(s) 752, 1045, 1120, 1145
AjnI CCWGG 4 cut(s) 275, 541, 1198, 1390
AjuI GAANNNNNNNTTGG 2 cut(s) 1412, 1444
AluBI AGCT 7 cut(s) 379, 815, 992, 1026, 1437, 1445, 1517
AluI AGCT 7 cut(s) 379, 815, 992, 1026, 1437, 1445, 1517
Alw21I GWGCWC 2 cut(s) 66, 381
Alw26I GTCTC 4 cut(s) 256, 609, 681, 711
AlwI GGATC 2 cut(s) 1454, 1679
AlwNI CAGNNNCTG 1 cut(s) 1391
AoxI GGCC 7 cut(s) 431, 868, 928, 1247, 1563, 1679, 1728
ApeKI GCWGC 4 cut(s) 97, 1442, 1445, 1689
ApoI RAATTY 2 cut(s) 759, 1405
AseI ATTAAT 1 cut(s) 606
AsiGI ACCGGT 2 cut(s) 528, 644
Asp718I GGTACC 2 cut(s) 411, 1398
AspLEI GCGC 4 cut(s) 215, 247, 403, 1336
AspS9I GGNCC 8 cut(s) 30, 220, 315, 334, 552, 630, 929, 1787
AsuC2I CCSGG 6 cut(s) 313, 350, 365, 443, 906, 1487
AsuHPI GGTGA 2 cut(s) 133, 536
AvaII GGWCC 7 cut(s) 30, 220, 315, 334, 552, 630, 1787
BalI TGGCCA 1 cut(s) 1249
BanI GGYRCC 3 cut(s) 400, 411, 1398
BanII GRGCYC 2 cut(s) 381, 1740
BarI GAAGNNNNNNTAC 2 cut(s) 1638, 1670
BbrPI CACGTG 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 1108
Bbv12I GWGCWC 2 cut(s) 66, 381
BbvCI CCTCAGC 1 cut(s) 1497
BbvI GCAGC 4 cut(s) 109, 1432, 1454, 1701
BccI CCATC 4 cut(s) 158, 644, 860, 1135
BceAI ACGGC 3 cut(s) 258, 406, 1347
BciT130I CCWGG 4 cut(s) 277, 543, 1200, 1392
BcnI CCSGG 6 cut(s) 313, 350, 365, 443, 906, 1487
BcoDI GTCTC 4 cut(s) 256, 609, 681, 711
BfaI CTAG 2 cut(s) 684, 1658
BfmI CTRYAG 3 cut(s) 765, 1224, 1263
BfoI RGCGCY 2 cut(s) 404, 1337
BfrI CTTAAG 2 cut(s) 1213, 1675
BfuAI ACCTGC 1 cut(s) 1217
BglI GCCNNNNNGGC 1 cut(s) 1255
BglII AGATCT 1 cut(s) 1806
BlpI GCTNAGC 2 cut(s) 993, 1821
BmcAI AGTACT 1 cut(s) 1829
Bme18I GGWCC 7 cut(s) 30, 220, 315, 334, 552, 630, 1787
BmgT120I GGNCC 8 cut(s) 30, 220, 315, 334, 552, 630, 929, 1787
BmiI GGNNCC 8 cut(s) 112, 317, 335, 402, 413, 553, 1400, 1788
BmsI GCATC 2 cut(s) 1735, 1740
BpiI GAAGAC 1 cut(s) 1108
BpmI CTGGAG 1 cut(s) 1221
Bpu10I CCTNAGC 1 cut(s) 1497
Bpu1102I GCTNAGC 2 cut(s) 993, 1821
BpuEI CTTGAG 4 cut(s) 305, 368, 793, 1196
BpuMI CCSGG 6 cut(s) 313, 350, 365, 443, 906, 1487
Bsa29I ATCGAT 1 cut(s) 639
BsaAI YACGTR 1 cut(s) 259
BsaHI GRCGYC 6 cut(s) 90, 273, 306, 401, 576, 1761
BsaWI WCCGGW 4 cut(s) 426, 528, 644, 1401
BsaXI ACNNNNNCTCC 2 cut(s) 1255, 1285
Bse118I RCCGGY 3 cut(s) 336, 528, 644
Bse1I ACTGG 5 cut(s) 947, 959, 1455, 1559, 1835
Bse3DI GCAATG 1 cut(s) 358
BseBI CCWGG 4 cut(s) 277, 543, 1200, 1392
BseCI ATCGAT 1 cut(s) 639
BseGI GGATG 5 cut(s) 1060, 1127, 1596, 1711, 1750
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 4 cut(s) 625, 984, 989, 1488
BseNI ACTGG 5 cut(s) 947, 959, 1455, 1559, 1835
BseRI GAGGAG 4 cut(s) 109, 158, 371, 1193
BseXI GCAGC 4 cut(s) 109, 1432, 1454, 1701
BsgI GTGCAG 2 cut(s) 52, 1431
Bsh1236I CGCG 2 cut(s) 213, 247
BshFI GGCC 7 cut(s) 433, 870, 930, 1249, 1565, 1681, 1730
BshNI GGYRCC 3 cut(s) 400, 411, 1398
BshTI ACCGGT 2 cut(s) 528, 644
BshVI ATCGAT 1 cut(s) 639
BsiHKAI GWGCWC 2 cut(s) 66, 381
BslFI GGGAC 4 cut(s) 301, 347, 565, 1469
BsmAI GTCTC 4 cut(s) 256, 609, 681, 711
BsmBI CGTCTC 1 cut(s) 609
BsmFI GGGAC 4 cut(s) 301, 347, 565, 1469
BsnI GGCC 7 cut(s) 433, 870, 930, 1249, 1565, 1681, 1730
Bsp1286I GDGCHC 3 cut(s) 66, 381, 1740
Bsp143I GATC 6 cut(s) 889, 1005, 1459, 1536, 1671, 1806
Bsp1720I GCTNAGC 2 cut(s) 993, 1821
BspANI GGCC 7 cut(s) 433, 870, 930, 1249, 1565, 1681, 1730
BspCNI CTCAG 4 cut(s) 624, 985, 988, 1489
BspDI ATCGAT 1 cut(s) 639
BspFNI CGCG 2 cut(s) 213, 247
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 8 cut(s) 112, 317, 335, 402, 413, 553, 1400, 1788
BspMAI CTGCAG 2 cut(s) 769, 1228
BspMI ACCTGC 1 cut(s) 1217
BspPI GGATC 2 cut(s) 1454, 1679
BspT107I GGYRCC 3 cut(s) 400, 411, 1398
BspTI CTTAAG 2 cut(s) 1213, 1675
BsrBI CCGCTC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 358
BsrFI RCCGGY 3 cut(s) 336, 528, 644
BsrI ACTGG 5 cut(s) 947, 959, 1455, 1559, 1835
BssAI RCCGGY 3 cut(s) 336, 528, 644
BssMI GATC 6 cut(s) 889, 1005, 1459, 1536, 1671, 1806
BssNAI GTATAC 1 cut(s) 1167
BssNI GRCGYC 6 cut(s) 90, 273, 306, 401, 576, 1761
BssT1I CCWWGG 4 cut(s) 851, 1368, 1510, 1731
Bst1107I GTATAC 1 cut(s) 1167
Bst2UI CCWGG 4 cut(s) 277, 543, 1200, 1392
Bst4CI ACNGT 5 cut(s) 61, 487, 700, 1097, 1267
Bst6I CTCTTC 2 cut(s) 80, 1776
BstACI GRCGYC 6 cut(s) 90, 273, 306, 401, 576, 1761
BstAFI CTTAAG 2 cut(s) 1213, 1675
BstBAI YACGTR 1 cut(s) 259
BstC8I GCNNGC 2 cut(s) 282, 1251
BstDEI CTNAG 5 cut(s) 611, 975, 993, 1497, 1821
BstDSI CCRYGG 1 cut(s) 486
BstEII GGTNACC 2 cut(s) 524, 671
BstENI CCTNNNNNAGG 1 cut(s) 546
BstF5I GGATG 5 cut(s) 1060, 1127, 1596, 1711, 1750
BstFNI CGCG 2 cut(s) 213, 247
BstH2I RGCGCY 2 cut(s) 404, 1337
BstHHI GCGC 4 cut(s) 215, 247, 403, 1336
BstKTI GATC 6 cut(s) 892, 1008, 1462, 1539, 1674, 1809
BstMAI GTCTC 4 cut(s) 256, 609, 681, 711
BstMBI GATC 6 cut(s) 889, 1005, 1459, 1536, 1671, 1806
BstMWI GCNNNNNNNGC 4 cut(s) 281, 337, 840, 1255
BstNI CCWGG 4 cut(s) 277, 543, 1200, 1392
BstPI GGTNACC 2 cut(s) 524, 671
BstSFI CTRYAG 3 cut(s) 765, 1224, 1263
BstUI CGCG 2 cut(s) 213, 247
BstV1I GCAGC 4 cut(s) 109, 1432, 1454, 1701
BstV2I GAAGAC 1 cut(s) 1108
BstX2I RGATCY 2 cut(s) 1671, 1806
BstXI CCANNNNNNTGG 1 cut(s) 942
BstYI RGATCY 2 cut(s) 1671, 1806
BstZ17I GTATAC 1 cut(s) 1167
Bsu15I ATCGAT 1 cut(s) 639
BsuRI GGCC 7 cut(s) 433, 870, 930, 1249, 1565, 1681, 1730
BsuTUI ATCGAT 1 cut(s) 639
BtgI CCRYGG 1 cut(s) 486
BtgZI GCGATG 1 cut(s) 1763
BtsCI GGATG 5 cut(s) 1060, 1127, 1596, 1711, 1750
BtsI GCAGTG 3 cut(s) 774, 829, 1193
BtsIMutI CAGTG 9 cut(s) 14, 66, 705, 774, 829, 952, 1093, 1193, 1448
BveI ACCTGC 1 cut(s) 1217
Cac8I GCNNGC 2 cut(s) 282, 1251
CaiI CAGNNNCTG 1 cut(s) 1391
CciI TCATGA 1 cut(s) 724
CfoI GCGC 4 cut(s) 215, 247, 403, 1336
Cfr10I RCCGGY 3 cut(s) 336, 528, 644
Cfr13I GGNCC 8 cut(s) 30, 220, 315, 334, 552, 630, 929, 1787
ClaI ATCGAT 1 cut(s) 639
CseI GACGC 5 cut(s) 79, 281, 314, 584, 1769
Csp6I GTAC 8 cut(s) 40, 289, 412, 457, 874, 1399, 1426, 1828
CspAI ACCGGT 2 cut(s) 528, 644
CviAII CATG 7 cut(s) 556, 725, 823, 1161, 1417, 1567, 1778
CviQI GTAC 8 cut(s) 40, 289, 412, 457, 874, 1399, 1426, 1828
DdeI CTNAG 5 cut(s) 611, 975, 993, 1497, 1821
DinI GGCGCC 1 cut(s) 402
DpnI GATC 6 cut(s) 891, 1007, 1461, 1538, 1673, 1808
DpnII GATC 6 cut(s) 889, 1005, 1459, 1536, 1671, 1806
EaeI YGGCCR 2 cut(s) 431, 1247
Eam1104I CTCTTC 2 cut(s) 80, 1776
EarI CTCTTC 2 cut(s) 80, 1776
EciI GGCGGA 3 cut(s) 94, 112, 136
Ecl136II GAGCTC 1 cut(s) 379
Eco130I CCWWGG 4 cut(s) 851, 1368, 1510, 1731
Eco24I GRGCYC 2 cut(s) 381, 1740
Eco47I GGWCC 7 cut(s) 30, 220, 315, 334, 552, 630, 1787
Eco53kI GAGCTC 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 38
Eco72I CACGTG 1 cut(s) 259
Eco91I GGTNACC 2 cut(s) 524, 671
EcoICRI GAGCTC 1 cut(s) 379
EcoNI CCTNNNNNAGG 1 cut(s) 546
EcoO109I RGGNCCY 2 cut(s) 220, 1787
EcoO65I GGTNACC 2 cut(s) 524, 671
EcoRI GAATTC 1 cut(s) 759
EcoRII CCWGG 4 cut(s) 275, 541, 1198, 1390
EcoT14I CCWWGG 4 cut(s) 851, 1368, 1510, 1731
EcoT38I GRGCYC 2 cut(s) 381, 1740
EgeI GGCGCC 1 cut(s) 402
EheI GGCGCC 1 cut(s) 402
ErhI CCWWGG 4 cut(s) 851, 1368, 1510, 1731
Esp3I CGTCTC 1 cut(s) 609
FaeI CATG 7 cut(s) 559, 728, 826, 1164, 1420, 1570, 1781
FalI AAGNNNNNCTT 2 cut(s) 1724, 1756
FaqI GGGAC 4 cut(s) 301, 347, 565, 1469
FatI CATG 7 cut(s) 555, 724, 822, 1160, 1416, 1566, 1777
FauI CCCGC 1 cut(s) 324
FblI GTMKAC 4 cut(s) 1166, 1571, 1647, 1773
FokI GGATG 5 cut(s) 1067, 1114, 1583, 1718, 1757
FriOI GRGCYC 2 cut(s) 381, 1740
FspBI CTAG 2 cut(s) 684, 1658
GlaI GCGC 4 cut(s) 214, 246, 402, 1335
GsuI CTGGAG 1 cut(s) 1221
HaeII RGCGCY 2 cut(s) 404, 1337
HaeIII GGCC 7 cut(s) 433, 870, 930, 1249, 1565, 1681, 1730
HgaI GACGC 5 cut(s) 79, 281, 314, 584, 1769
HhaI GCGC 4 cut(s) 215, 247, 403, 1336
Hin1I GRCGYC 6 cut(s) 90, 273, 306, 401, 576, 1761
Hin1II CATG 7 cut(s) 559, 728, 826, 1164, 1420, 1570, 1781
Hin6I GCGC 4 cut(s) 213, 245, 401, 1334
HinP1I GCGC 4 cut(s) 213, 245, 401, 1334
HincII GTYRAC 2 cut(s) 483, 1504
HindII GTYRAC 2 cut(s) 483, 1504
HindIII AAGCTT 2 cut(s) 1024, 1435
HinfI GANTC 8 cut(s) 394, 499, 537, 919, 1189, 1302, 1607, 1877
HphI GGTGA 2 cut(s) 133, 536
Hpy188I TCNGA 6 cut(s) 504, 614, 1322, 1541, 1551, 1606
Hpy188III TCNNGA 9 cut(s) 628, 684, 725, 749, 810, 1009, 1120, 1151, 1658
Hpy99I CGWCG 2 cut(s) 244, 275
HpyAV CCTTC 4 cut(s) 1045, 1292, 1720, 1853
HpyCH4III ACNGT 5 cut(s) 61, 487, 700, 1097, 1267
HpyCH4IV ACGT 3 cut(s) 258, 465, 1528
HpyCH4V TGCA 9 cut(s) 69, 659, 710, 767, 1226, 1355, 1442, 1448, 1753
HpyF10VI GCNNNNNNNGC 4 cut(s) 281, 337, 840, 1255
HpyF3I CTNAG 5 cut(s) 611, 975, 993, 1497, 1821
HpySE526I ACGT 3 cut(s) 258, 465, 1528
Hsp92I GRCGYC 6 cut(s) 90, 273, 306, 401, 576, 1761
Hsp92II CATG 7 cut(s) 559, 728, 826, 1164, 1420, 1570, 1781
HspAI GCGC 4 cut(s) 213, 245, 401, 1334
KasI GGCGCC 1 cut(s) 400
KpnI GGTACC 2 cut(s) 415, 1402
Kzo9I GATC 6 cut(s) 889, 1005, 1459, 1536, 1671, 1806
LmnI GCTCC 2 cut(s) 384, 1514
Lsp1109I GCAGC 4 cut(s) 109, 1432, 1454, 1701
LweI GCATC 2 cut(s) 1735, 1740
MaeI CTAG 2 cut(s) 684, 1658
MaeII ACGT 3 cut(s) 258, 465, 1528
MaeIII GTNAC 3 cut(s) 139, 524, 671
MalI GATC 6 cut(s) 891, 1007, 1461, 1538, 1673, 1808
MbiI CCGCTC 1 cut(s) 331
MboI GATC 6 cut(s) 889, 1005, 1459, 1536, 1671, 1806
MboII GAAGA 7 cut(s) 67, 382, 505, 508, 1108, 1646, 1793
MflI RGATCY 2 cut(s) 1671, 1806
MhlI GDGCHC 3 cut(s) 66, 381, 1740
MlsI TGGCCA 1 cut(s) 1249
MluCI AATT 8 cut(s) 84, 505, 759, 1040, 1235, 1278, 1405, 1695
MluNI TGGCCA 1 cut(s) 1249
Mly113I GGCGCC 1 cut(s) 401
MlyI GAGTC 1 cut(s) 1183
Mox20I TGGCCA 1 cut(s) 1249
MscI TGGCCA 1 cut(s) 1249
MseI TTAA 5 cut(s) 47, 606, 695, 1214, 1676
Msp20I TGGCCA 1 cut(s) 1249
MspA1I CMGCKG 1 cut(s) 1445
MspCI CTTAAG 2 cut(s) 1213, 1675
MvaI CCWGG 4 cut(s) 277, 543, 1200, 1392
MvnI CGCG 2 cut(s) 213, 247
MwoI GCNNNNNNNGC 4 cut(s) 281, 337, 840, 1255
NarI GGCGCC 1 cut(s) 401
NciI CCSGG 6 cut(s) 313, 350, 365, 443, 906, 1487
NdeII GATC 6 cut(s) 889, 1005, 1459, 1536, 1671, 1806
NlaIII CATG 7 cut(s) 559, 728, 826, 1164, 1420, 1570, 1781
NlaIV GGNNCC 8 cut(s) 112, 317, 335, 402, 413, 553, 1400, 1788
NmeAIII GCCGAG 2 cut(s) 241, 310
NmuCI GTSAC 2 cut(s) 139, 524
PagI TCATGA 1 cut(s) 724
PaqCI CACCTGC 1 cut(s) 1217
PasI CCCWGGG 2 cut(s) 542, 1391
PcsI WCGNNNNNNNCGW 1 cut(s) 898
PfeI GAWTC 7 cut(s) 394, 499, 537, 919, 1302, 1607, 1877
PflMI CCANNNNNTGG 1 cut(s) 1199
PinAI ACCGGT 2 cut(s) 528, 644
PleI GAGTC 1 cut(s) 1183
PluTI GGCGCC 1 cut(s) 404
PmaCI CACGTG 1 cut(s) 259
PmlI CACGTG 1 cut(s) 259
PpsI GAGTC 1 cut(s) 1183
Ppu21I YACGTR 1 cut(s) 259
PpuMI RGGWCCY 2 cut(s) 220, 1787
PshBI ATTAAT 1 cut(s) 606
PsiI TTATAA 1 cut(s) 1362
Psp124BI GAGCTC 1 cut(s) 381
Psp5II RGGWCCY 2 cut(s) 220, 1787
Psp6I CCWGG 4 cut(s) 275, 541, 1198, 1390
PspCI CACGTG 1 cut(s) 259
PspEI GGTNACC 2 cut(s) 524, 671
PspGI CCWGG 4 cut(s) 275, 541, 1198, 1390
PspN4I GGNNCC 8 cut(s) 112, 317, 335, 402, 413, 553, 1400, 1788
PspPI GGNCC 8 cut(s) 30, 220, 315, 334, 552, 630, 929, 1787
PspPPI RGGWCCY 2 cut(s) 220, 1787
PstI CTGCAG 2 cut(s) 769, 1228
PstNI CAGNNNCTG 1 cut(s) 1391
PsuI RGATCY 2 cut(s) 1671, 1806
PvuII CAGCTG 1 cut(s) 1445
RsaI GTAC 8 cut(s) 41, 290, 413, 458, 875, 1400, 1427, 1829
RsaNI GTAC 8 cut(s) 40, 289, 412, 457, 874, 1399, 1426, 1828
SacI GAGCTC 1 cut(s) 381
SaqAI TTAA 5 cut(s) 47, 606, 695, 1214, 1676
Sau3AI GATC 6 cut(s) 889, 1005, 1459, 1536, 1671, 1806
Sau96I GGNCC 8 cut(s) 30, 220, 315, 334, 552, 630, 929, 1787
ScaI AGTACT 1 cut(s) 1829
SchI GAGTC 1 cut(s) 1183
SduI GDGCHC 3 cut(s) 66, 381, 1740
SfaNI GCATC 2 cut(s) 1735, 1740
SfcI CTRYAG 3 cut(s) 765, 1224, 1263
SfoI GGCGCC 1 cut(s) 402
SinI GGWCC 7 cut(s) 30, 220, 315, 334, 552, 630, 1787
SmlI CTYRAG 6 cut(s) 320, 383, 808, 1175, 1213, 1675
SmoI CTYRAG 6 cut(s) 320, 383, 808, 1175, 1213, 1675
Sse9I AATT 8 cut(s) 84, 505, 759, 1040, 1235, 1278, 1405, 1695
SspDI GGCGCC 1 cut(s) 400
SspMI CTAG 2 cut(s) 684, 1658
SstI GAGCTC 1 cut(s) 381
StyI CCWWGG 4 cut(s) 851, 1368, 1510, 1731
TaaI ACNGT 5 cut(s) 61, 487, 700, 1097, 1267
TaiI ACGT 3 cut(s) 261, 468, 1531
TaqI TCGA 7 cut(s) 192, 327, 535, 639, 819, 1008, 1455
TasI AATT 8 cut(s) 84, 505, 759, 1040, 1235, 1278, 1405, 1695
TatI WGTACW 4 cut(s) 39, 288, 873, 1827
TauI GCSGC 6 cut(s) 128, 203, 265, 406, 472, 1820
TfiI GAWTC 7 cut(s) 394, 499, 537, 919, 1302, 1607, 1877
Tru1I TTAA 5 cut(s) 47, 606, 695, 1214, 1676
Tru9I TTAA 5 cut(s) 47, 606, 695, 1214, 1676
TscAI CASTG 9 cut(s) 21, 66, 705, 774, 836, 959, 1100, 1200, 1455
TseFI GTSAC 2 cut(s) 139, 524
TseI GCWGC 4 cut(s) 97, 1442, 1445, 1689
Tsp45I GTSAC 2 cut(s) 139, 524
TspDTI ATGAA 7 cut(s) 741, 1433, 1490, 1539, 1585, 1794, 1872
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 9 cut(s) 21, 66, 705, 774, 836, 959, 1100, 1200, 1455
Van91I CCANNNNNTGG 1 cut(s) 1199
Vha464I CTTAAG 2 cut(s) 1213, 1675
VpaK11BI GGWCC 7 cut(s) 30, 220, 315, 334, 552, 630, 1787
VspI ATTAAT 1 cut(s) 606
XagI CCTNNNNNAGG 1 cut(s) 546
XapI RAATTY 2 cut(s) 759, 1405
XbaI TCTAGA 2 cut(s) 683, 1657
XcmI CCANNNNNNNNNTGG 3 cut(s) 939, 1158, 1738
XmiI GTMKAC 4 cut(s) 1166, 1571, 1647, 1773
XspI CTAG 2 cut(s) 684, 1658
ZrmI AGTACT 1 cut(s) 1829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.