Rw1G021310

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009758
Physical Location & Seq
Reverse (-)
45545239 .. 45547133
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021310.1

Sequence Viewer

Length: 1203 bp
ATGTCTAGGGACATTTTAACAGTGTCATTTGCACCAAGAGACACTCATGAAGCACAAGTTCAATTTGCTCTTGAAAGAGGAGTTCCTGCAGTGATTGGAGTAATGGCTTCAATTAGACTTCCCTATCCCTCGAGAGCTTTCGACATGGCTCACTGCTCTCGTTGTCTCATTCCGTGGGGCCAATATGATGGGCTGTACTTGATTGAAGTGGATCGTGTTTTACGACCCGGGGGGTATTGGATTCTGTCCGGCCCACCAATCAACTGGCAGAATCACTGGAAAGGTTGGCAAAGAACTGAGGCAGACCTCAAAGCTGAGCAGGACATGATTGAGAGAGTAGCAAAAAGCTTATGCTGGAAAAAATTGAAGCAGAAGGGTGACCTTGCCATTTGGCAAAAACCCACTAACCATATCCACTGTCAAGCCAATAGAAAGGTCTTCAAGAAACCATCCTTCTGCCAAGCTCAAAATCCAGACACAGCATGGTATACTAAACTTGAGGAGTGTTTGACTCCATTGCCCGGAGCGAACAACATAAAAGAAGTTGCTGGTGGGGAATTGGCTAAATGGCCAGCGAGGCTAACTACAGTTCCTCCAAGAATTAGTAGTGGAAGTTTGAAAGGAATTACAGCTGAGATGTTCAGGGAAAACACGGCGCTATGGAAACAGAGAGTTGCACATTATAAGACCTTGGACTATCAGTTGGCAGACCCTGGGAGGTATCGTAATTTGCTTGACATGAACTCTTACTTGGGGAGCTTTGCGGCTGCACTGGCTGATGACCCTGCATGGGTTATGAACATTGTCCCCGTTGAGGCTGAGGTGAACACCCTCGGAGCCATTTATGAACGTGGATTGATTGGAACTTATCAGAATTGGTGTGAGGCCATGTCTACTTACCCAAGAACCTATGACTTCATCCACTCGGATTCCGTTTTCACTCTTTACAAGGACAGATGCGAAATGGAAGACATTCTTCTAGAAATGGATAGGATTTTAAGGCCACAAGGCAGTATAATTTTCAGGGATGATGTGGATTTGGTTCTGAAGGCCAAGGCGCTCTTAGATGCAATGCAATATGATGCCAGAATTGTAGACCACGAAAAGAGTCCTCATCATAGAGAAAAGATATTGTTGGCGGCTAAGCAGTACTGGACAGCACCGGCACCAAACCACGAAGGAGAAGGCAGTAAAGAATCATAG

Protein Analysis

400

Amino Acids

45.65

Weight (kDa)

7.15

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 1 - 382 2.1e-158 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 684
AccB1I GGYRCC 1 cut(s) 1165
AccI GTMKAC 3 cut(s) 488, 893, 1095
AciI CCGC 2 cut(s) 764, 1139
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 1 cut(s) 569
AcuI CTGAAG 1 cut(s) 1067
AfaI GTAC 2 cut(s) 197, 1151
AfiI CCNNNNNNNGG 3 cut(s) 521, 790, 814
AgsI TTSAA 7 cut(s) 62, 74, 111, 206, 367, 442, 619
AjnI CCWGG 1 cut(s) 712
AjuI GAANNNNNNNTTGG 2 cut(s) 734, 766
AluBI AGCT 6 cut(s) 137, 314, 348, 464, 632, 759
AluI AGCT 6 cut(s) 137, 314, 348, 464, 632, 759
Alw26I GTCTC 2 cut(s) 33, 170
AlwI GGATC 1 cut(s) 219
AlwNI CAGNNNCTG 1 cut(s) 713
Ama87I CYCGRG 2 cut(s) 130, 227
AoxI GGCC 6 cut(s) 178, 250, 569, 885, 1001, 1050
ApeKI GCWGC 1 cut(s) 767
Asp700I GAANNNNTTC 1 cut(s) 972
AspLEI GCGC 2 cut(s) 658, 1060
AspS9I GGNCC 2 cut(s) 178, 251
AsuC2I CCSGG 3 cut(s) 228, 229, 522
AsuHPI GGTGA 2 cut(s) 389, 835
AvaI CYCGRG 2 cut(s) 130, 227
BalI TGGCCA 1 cut(s) 571
BanI GGYRCC 1 cut(s) 1165
BbsI GAAGAC 2 cut(s) 430, 975
BbvCI CCTCAGC 1 cut(s) 819
BbvI GCAGC 1 cut(s) 754
BccI CCATC 2 cut(s) 182, 457
BceAI ACGGC 1 cut(s) 669
BciT130I CCWGG 1 cut(s) 714
BcnI CCSGG 3 cut(s) 228, 229, 522
BcoDI GTCTC 2 cut(s) 33, 170
BfaI CTAG 2 cut(s) 6, 980
BfmI CTRYAG 2 cut(s) 87, 585
BfoI RGCGCY 2 cut(s) 659, 1061
BglI GCCNNNNNGGC 1 cut(s) 577
BisI GCNGC 3 cut(s) 765, 768, 1140
BlpI GCTNAGC 2 cut(s) 315, 1143
BlsI GCNGC 3 cut(s) 766, 769, 1141
BmcAI AGTACT 1 cut(s) 1151
Bme1390I CCNGG 4 cut(s) 228, 229, 522, 714
BmeT110I CYCGRG 2 cut(s) 130, 227
BmgT120I GGNCC 2 cut(s) 178, 251
BmiI GGNNCC 3 cut(s) 179, 838, 1167
BmrFI CCNGG 4 cut(s) 228, 229, 522, 714
BmsI GCATC 3 cut(s) 947, 1057, 1072
BpiI GAAGAC 2 cut(s) 430, 975
Bpu10I CCTNAGC 1 cut(s) 819
Bpu1102I GCTNAGC 2 cut(s) 315, 1143
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 3 cut(s) 228, 229, 522
BsaJI CCNNGG 8 cut(s) 173, 227, 228, 690, 712, 713, 832, 1053
BsaXI ACNNNNNCTCC 4 cut(s) 577, 607, 709, 739
Bsc4I CCNNNNNNNGG 3 cut(s) 521, 790, 814
Bse118I RCCGGY 1 cut(s) 1162
Bse1I ACTGG 4 cut(s) 269, 281, 777, 1157
Bse3DI GCAATG 2 cut(s) 515, 1077
BseBI CCWGG 1 cut(s) 714
BseDI CCNNGG 8 cut(s) 173, 227, 228, 690, 712, 713, 832, 1053
BseGI GGATG 3 cut(s) 449, 918, 1033
BseLI CCNNNNNNNGG 3 cut(s) 521, 790, 814
BseMI GCAATG 2 cut(s) 515, 1077
BseMII CTCAG 4 cut(s) 288, 306, 624, 810
BseNI ACTGG 4 cut(s) 269, 281, 777, 1157
BseRI GAGGAG 2 cut(s) 93, 515
BseXI GCAGC 1 cut(s) 754
BsgI GTGCAG 1 cut(s) 753
BshFI GGCC 6 cut(s) 180, 252, 571, 887, 1003, 1052
BshNI GGYRCC 1 cut(s) 1165
BsiHKCI CYCGRG 2 cut(s) 130, 227
BsiSI CCGG 4 cut(s) 228, 249, 522, 1163
BslFI GGGAC 2 cut(s) 23, 791
BslI CCNNNNNNNGG 3 cut(s) 521, 790, 814
BsmAI GTCTC 2 cut(s) 33, 170
BsmFI GGGAC 2 cut(s) 23, 791
BsnI GGCC 6 cut(s) 180, 252, 571, 887, 1003, 1052
BsoBI CYCGRG 2 cut(s) 130, 227
Bsp143I GATC 1 cut(s) 211
Bsp1720I GCTNAGC 2 cut(s) 315, 1143
BspACI CCGC 2 cut(s) 764, 1139
BspANI GGCC 6 cut(s) 180, 252, 571, 887, 1003, 1052
BspCNI CTCAG 4 cut(s) 289, 307, 625, 811
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 179, 838, 1167
BspMAI CTGCAG 1 cut(s) 91
BspPI GGATC 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 1165
BsrDI GCAATG 2 cut(s) 515, 1077
BsrFI RCCGGY 1 cut(s) 1162
BsrI ACTGG 4 cut(s) 269, 281, 777, 1157
BssAI RCCGGY 1 cut(s) 1162
BssECI CCNNGG 8 cut(s) 173, 227, 228, 690, 712, 713, 832, 1053
BssMI GATC 1 cut(s) 211
BssNAI GTATAC 1 cut(s) 489
BssT1I CCWWGG 2 cut(s) 690, 1053
Bst1107I GTATAC 1 cut(s) 489
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 3 cut(s) 22, 419, 589
BstC8I GCNNGC 1 cut(s) 573
BstDEI CTNAG 6 cut(s) 297, 315, 633, 819, 1063, 1143
BstDSI CCRYGG 1 cut(s) 173
BstEII GGTNACC 1 cut(s) 377
BstF5I GGATG 3 cut(s) 449, 918, 1033
BstH2I RGCGCY 2 cut(s) 659, 1061
BstHHI GCGC 2 cut(s) 658, 1060
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 2 cut(s) 33, 170
BstMBI GATC 1 cut(s) 211
BstMWI GCNNNNNNNGC 2 cut(s) 577, 773
BstNI CCWGG 1 cut(s) 714
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 4 cut(s) 226, 227, 520, 712
BstSFI CTRYAG 2 cut(s) 87, 585
BstV1I GCAGC 1 cut(s) 754
BstV2I GAAGAC 2 cut(s) 430, 975
BstXI CCANNNNNNTGG 2 cut(s) 188, 264
BstZ17I GTATAC 1 cut(s) 489
BsuRI GGCC 6 cut(s) 180, 252, 571, 887, 1003, 1052
BtgI CCRYGG 1 cut(s) 173
BtsCI GGATG 3 cut(s) 449, 918, 1033
BtsI GCAGTG 2 cut(s) 96, 151
BtsIMutI CAGTG 6 cut(s) 27, 96, 151, 274, 415, 770
Cac8I GCNNGC 1 cut(s) 573
CaiI CAGNNNCTG 1 cut(s) 713
CciI TCATGA 1 cut(s) 46
CfoI GCGC 2 cut(s) 658, 1060
Cfr10I RCCGGY 1 cut(s) 1162
Cfr13I GGNCC 2 cut(s) 178, 251
Cfr9I CCCGGG 1 cut(s) 227
Csp6I GTAC 2 cut(s) 196, 1150
CviAII CATG 7 cut(s) 47, 145, 325, 483, 739, 789, 889
CviQI GTAC 2 cut(s) 196, 1150
DdeI CTNAG 6 cut(s) 297, 315, 633, 819, 1063, 1143
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 569
Eco130I CCWWGG 2 cut(s) 690, 1053
Eco57I CTGAAG 1 cut(s) 1067
Eco88I CYCGRG 2 cut(s) 130, 227
Eco91I GGTNACC 1 cut(s) 377
EcoO65I GGTNACC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 712
EcoT14I CCWWGG 2 cut(s) 690, 1053
ErhI CCWWGG 2 cut(s) 690, 1053
FaeI CATG 7 cut(s) 50, 148, 328, 486, 742, 792, 892
FalI AAGNNNNNCTT 4 cut(s) 960, 992, 1046, 1078
FaqI GGGAC 2 cut(s) 23, 791
FatI CATG 7 cut(s) 46, 144, 324, 482, 738, 788, 888
FblI GTMKAC 3 cut(s) 488, 893, 1095
Fnu4HI GCNGC 3 cut(s) 765, 768, 1140
FokI GGATG 3 cut(s) 436, 905, 1040
Fsp4HI GCNGC 3 cut(s) 765, 768, 1140
FspBI CTAG 2 cut(s) 6, 980
GlaI GCGC 2 cut(s) 657, 1059
GluI GCNGC 3 cut(s) 765, 768, 1140
HaeII RGCGCY 2 cut(s) 659, 1061
HaeIII GGCC 6 cut(s) 180, 252, 571, 887, 1003, 1052
HapII CCGG 4 cut(s) 228, 249, 522, 1163
HhaI GCGC 2 cut(s) 658, 1060
Hin1II CATG 7 cut(s) 50, 148, 328, 486, 742, 792, 892
Hin6I GCGC 2 cut(s) 656, 1058
HinP1I GCGC 2 cut(s) 656, 1058
HindIII AAGCTT 1 cut(s) 346
HinfI GANTC 6 cut(s) 241, 271, 511, 929, 1108, 1196
HpaII CCGG 4 cut(s) 228, 249, 522, 1163
HphI GGTGA 2 cut(s) 389, 835
Hpy166II GTNNAC 4 cut(s) 489, 826, 894, 1096
Hpy188I TCNGA 4 cut(s) 836, 873, 928, 1047
Hpy188III TCNNGA 6 cut(s) 47, 71, 132, 442, 473, 980
Hpy8I GTNNAC 4 cut(s) 489, 826, 894, 1096
HpyAV CCTTC 5 cut(s) 367, 463, 1042, 1172, 1178
HpyCH4III ACNGT 3 cut(s) 22, 419, 589
HpyCH4IV ACGT 1 cut(s) 850
HpyCH4V TGCA 7 cut(s) 32, 89, 677, 770, 788, 1070, 1075
HpyF10VI GCNNNNNNNGC 2 cut(s) 577, 773
HpyF3I CTNAG 6 cut(s) 297, 315, 633, 819, 1063, 1143
HpySE526I ACGT 1 cut(s) 850
Hsp92II CATG 7 cut(s) 50, 148, 328, 486, 742, 792, 892
HspAI GCGC 2 cut(s) 656, 1058
Kzo9I GATC 1 cut(s) 211
LmnI GCTCC 3 cut(s) 524, 756, 836
Lsp1109I GCAGC 1 cut(s) 754
LweI GCATC 3 cut(s) 947, 1057, 1072
MaeI CTAG 2 cut(s) 6, 980
MaeII ACGT 1 cut(s) 850
MaeIII GTNAC 1 cut(s) 377
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 3 cut(s) 430, 968, 980
MlsI TGGCCA 1 cut(s) 571
MluNI TGGCCA 1 cut(s) 571
MlyI GAGTC 2 cut(s) 505, 1117
Mox20I TGGCCA 1 cut(s) 571
MroXI GAANNNNTTC 1 cut(s) 972
MscI TGGCCA 1 cut(s) 571
MseI TTAA 2 cut(s) 17, 998
Msp20I TGGCCA 1 cut(s) 571
MspA1I CMGCKG 1 cut(s) 632
MspI CCGG 4 cut(s) 228, 249, 522, 1163
MspR9I CCNGG 4 cut(s) 228, 229, 522, 714
MvaI CCWGG 1 cut(s) 714
MwoI GCNNNNNNNGC 2 cut(s) 577, 773
NciI CCSGG 3 cut(s) 228, 229, 522
NdeII GATC 1 cut(s) 211
NlaIII CATG 7 cut(s) 50, 148, 328, 486, 742, 792, 892
NlaIV GGNNCC 3 cut(s) 179, 838, 1167
NmuCI GTSAC 1 cut(s) 377
PaeR7I CTCGAG 1 cut(s) 130
PagI TCATGA 1 cut(s) 46
PasI CCCWGGG 1 cut(s) 713
PcsI WCGNNNNNNNCGW 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 972
PfeI GAWTC 4 cut(s) 241, 271, 929, 1196
PkrI GCNGC 3 cut(s) 766, 769, 1141
PleI GAGTC 2 cut(s) 505, 1116
PpsI GAGTC 2 cut(s) 505, 1116
PsiI TTATAA 1 cut(s) 684
Psp6I CCWGG 1 cut(s) 712
PspEI GGTNACC 1 cut(s) 377
PspGI CCWGG 1 cut(s) 712
PspN4I GGNNCC 3 cut(s) 179, 838, 1167
PspPI GGNCC 2 cut(s) 178, 251
PstI CTGCAG 1 cut(s) 91
PstNI CAGNNNCTG 1 cut(s) 713
PvuII CAGCTG 1 cut(s) 632
RsaI GTAC 2 cut(s) 197, 1151
RsaNI GTAC 2 cut(s) 196, 1150
SaqAI TTAA 2 cut(s) 17, 998
SatI GCNGC 3 cut(s) 765, 768, 1140
Sau3AI GATC 1 cut(s) 211
Sau96I GGNCC 2 cut(s) 178, 251
ScaI AGTACT 1 cut(s) 1151
SchI GAGTC 2 cut(s) 505, 1117
ScrFI CCNGG 4 cut(s) 228, 229, 522, 714
SfaNI GCATC 3 cut(s) 947, 1057, 1072
SfcI CTRYAG 2 cut(s) 87, 585
Sfr274I CTCGAG 1 cut(s) 130
SlaI CTCGAG 1 cut(s) 130
SmaI CCCGGG 1 cut(s) 229
SmlI CTYRAG 2 cut(s) 130, 497
SmoI CTYRAG 2 cut(s) 130, 497
SsiI CCGC 2 cut(s) 764, 1139
SspMI CTAG 2 cut(s) 6, 980
StyD4I CCNGG 4 cut(s) 226, 227, 520, 712
StyI CCWWGG 2 cut(s) 690, 1053
TaaI ACNGT 3 cut(s) 22, 419, 589
TaiI ACGT 1 cut(s) 853
TaqI TCGA 2 cut(s) 131, 141
TatI WGTACW 2 cut(s) 195, 1149
TauI GCSGC 2 cut(s) 767, 1142
TfiI GAWTC 4 cut(s) 241, 271, 929, 1196
Tru1I TTAA 2 cut(s) 17, 998
Tru9I TTAA 2 cut(s) 17, 998
TscAI CASTG 6 cut(s) 27, 96, 158, 281, 422, 777
TseFI GTSAC 1 cut(s) 377
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 1 cut(s) 377
TspDTI ATGAA 5 cut(s) 63, 755, 812, 861, 907
TspGWI ACGGA 2 cut(s) 162, 922
TspMI CCCGGG 1 cut(s) 227
TspRI CASTG 6 cut(s) 27, 96, 158, 281, 422, 777
XbaI TCTAGA 1 cut(s) 979
XcmI CCANNNNNNNNNTGG 2 cut(s) 261, 480
XhoI CTCGAG 1 cut(s) 130
XmaI CCCGGG 1 cut(s) 227
XmiI GTMKAC 3 cut(s) 488, 893, 1095
XmnI GAANNNNTTC 1 cut(s) 972
XspI CTAG 2 cut(s) 6, 980
ZrmI AGTACT 1 cut(s) 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.