Rorug01G0230000

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009758
Physical Location & Seq
Forward (+)
33202447 .. 33202830
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0230000.1

Sequence Viewer

Length: 384 bp
ATGGAACCACAAACCCAACAACCAACCGAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTTATAAGCGGAGTAGGTGGACTCCCACTACAGATCAGATAAGAATCCTCGAGCTTTTCTACAATAAGGGAGTTAGGTCCCCAACTGTAGAGCAGGTTGAGAGGATCTATCTCCAGCTGAAATGGTACGGCAAGATCGAGTTCAAGAACGTCTATTTTTGGTTCGTGAACCAAAGGGCTCGGGAGAAGCAGAAGAAGAAGTCCACTTCGGATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTGATGGCAATGTTGCCAATTCATCTGCACCTGCTTCTGCTTCTTCTGCTGGTGTGATCTGTTAA

Protein Analysis

127

Amino Acids

14.27

Weight (kDa)

9.71

Isoelectric Point (pI)

58.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 27 - 88 2.3e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 78
AarI CACCTGC 1 cut(s) 358
Acc36I ACCTGC 2 cut(s) 157, 358
AciI CCGC 1 cut(s) 82
AclWI GGATC 1 cut(s) 185
AfaI GTAC 1 cut(s) 200
AgsI TTSAA 1 cut(s) 217
AluBI AGCT 2 cut(s) 127, 190
AluI AGCT 2 cut(s) 127, 190
AlwI GGATC 1 cut(s) 185
Ama87I CYCGRG 2 cut(s) 122, 252
ApeKI GCWGC 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 150
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 332
AvaI CYCGRG 2 cut(s) 122, 252
AvaII GGWCC 1 cut(s) 150
BaeGI GKGCMC 1 cut(s) 297
BanII GRGCYC 1 cut(s) 253
BbvI GCAGC 1 cut(s) 62
BccI CCATC 2 cut(s) 26, 317
BceAI ACGGC 1 cut(s) 217
BcnI CCSGG 1 cut(s) 55
BfmI CTRYAG 2 cut(s) 102, 159
BfuAI ACCTGC 2 cut(s) 157, 358
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 55
Bme18I GGWCC 1 cut(s) 150
BmeT110I CYCGRG 2 cut(s) 122, 252
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 2 cut(s) 6, 152
BmrFI CCNGG 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 170
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 27
Bse3DI GCAATG 1 cut(s) 334
Bse8I GATNNNNATC 1 cut(s) 116
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 2 cut(s) 37, 289
BseJI GATNNNNATC 1 cut(s) 116
BseMI GCAATG 1 cut(s) 334
BseSI GKGCMC 1 cut(s) 297
BseXI GCAGC 1 cut(s) 62
BsgI GTGCAG 1 cut(s) 330
BsiHKCI CYCGRG 2 cut(s) 122, 252
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 136
BsmFI GGGAC 1 cut(s) 136
BsoBI CYCGRG 2 cut(s) 122, 252
Bsp1286I GDGCHC 2 cut(s) 253, 297
Bsp143I GATC 5 cut(s) 106, 177, 207, 305, 375
BspACI CCGC 1 cut(s) 82
BspLI GGNNCC 2 cut(s) 6, 152
BspMI ACCTGC 2 cut(s) 157, 358
BspPI GGATC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 5 cut(s) 106, 177, 207, 305, 375
Bst4CI ACNGT 1 cut(s) 160
BstF5I GGATG 2 cut(s) 37, 289
BstKTI GATC 5 cut(s) 109, 180, 210, 308, 378
BstMBI GATC 5 cut(s) 106, 177, 207, 305, 375
BstMWI GCNNNNNNNGC 2 cut(s) 79, 365
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 53
BstSFI CTRYAG 2 cut(s) 102, 159
BstSLI GKGCMC 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 62
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BtsCI GGATG 2 cut(s) 37, 289
BveI ACCTGC 2 cut(s) 157, 358
Cfr13I GGNCC 1 cut(s) 150
Csp6I GTAC 1 cut(s) 199
CviAII CATG 3 cut(s) 70, 290, 298
CviJI RGCY 5 cut(s) 53, 127, 190, 251, 313
CviKI_1 RGCY 5 cut(s) 53, 127, 190, 251, 313
CviQI GTAC 1 cut(s) 199
DpnI GATC 5 cut(s) 108, 179, 209, 307, 377
DpnII GATC 5 cut(s) 106, 177, 207, 305, 375
Eco24I GRGCYC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 150
Eco88I CYCGRG 2 cut(s) 122, 252
EcoO109I RGGNCCY 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 253
FaeI CATG 3 cut(s) 73, 293, 301
FaiI YATR 4 cut(s) 71, 78, 291, 299
FaqI GGGAC 1 cut(s) 136
FatI CATG 3 cut(s) 69, 289, 297
Fnu4HI GCNGC 1 cut(s) 51
FokI GGATG 2 cut(s) 44, 296
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 1 cut(s) 51
GluI GCNGC 1 cut(s) 51
GsuI CTGGAG 1 cut(s) 170
HapII CCGG 1 cut(s) 54
Hin1II CATG 3 cut(s) 73, 293, 301
HinfI GANTC 2 cut(s) 94, 117
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 3 cut(s) 93, 241, 276
Hpy188I TCNGA 2 cut(s) 111, 283
Hpy188III TCNNGA 3 cut(s) 217, 238, 254
Hpy8I GTNNAC 3 cut(s) 93, 241, 276
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 2 cut(s) 301, 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 365
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 3 cut(s) 73, 293, 301
Kzo9I GATC 5 cut(s) 106, 177, 207, 305, 375
LpnPI CCDG 6 cut(s) 67, 152, 200, 294, 354, 363
Lsp1109I GCAGC 1 cut(s) 62
MaeII ACGT 1 cut(s) 222
MalI GATC 5 cut(s) 108, 179, 209, 307, 377
MboI GATC 5 cut(s) 106, 177, 207, 305, 375
MboII GAAGA 3 cut(s) 277, 280, 354
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 2 cut(s) 253, 297
MluCI AATT 1 cut(s) 337
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 4 cut(s) 22, 29, 131, 168
MseI TTAA 1 cut(s) 382
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 1 cut(s) 55
MwoI GCNNNNNNNGC 2 cut(s) 79, 365
NciI CCSGG 1 cut(s) 55
NdeII GATC 5 cut(s) 106, 177, 207, 305, 375
NlaIII CATG 3 cut(s) 73, 293, 301
NlaIV GGNNCC 2 cut(s) 6, 152
NspI RCATGY 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 122
PaqCI CACCTGC 1 cut(s) 358
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 1 cut(s) 117
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 150
PsiI TTATAA 1 cut(s) 78
Psp5II RGGWCCY 1 cut(s) 150
PspN4I GGNNCC 2 cut(s) 6, 152
PspPI GGNCC 1 cut(s) 150
PspPPI RGGWCCY 1 cut(s) 150
PspXI VCTCGAGB 1 cut(s) 122
PsuI RGATCY 1 cut(s) 177
PvuII CAGCTG 1 cut(s) 190
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
SaqAI TTAA 1 cut(s) 382
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 5 cut(s) 106, 177, 207, 305, 375
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 55
SduI GDGCHC 2 cut(s) 253, 297
SetI ASST 7 cut(s) 92, 129, 152, 171, 192, 225, 352
SfcI CTRYAG 2 cut(s) 102, 159
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 150
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 337
SsiI CCGC 1 cut(s) 82
StyD4I CCNGG 1 cut(s) 53
TaaI ACNGT 1 cut(s) 160
TaiI ACGT 1 cut(s) 225
TaqI TCGA 2 cut(s) 123, 210
TasI AATT 1 cut(s) 337
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 1 cut(s) 382
Tru9I TTAA 1 cut(s) 382
TseI GCWGC 1 cut(s) 50
TspDTI ATGAA 2 cut(s) 278, 330
VpaK11BI GGWCC 1 cut(s) 150
XceI RCATGY 1 cut(s) 73
XhoI CTCGAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.