Rw1G021280

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009758
Physical Location & Seq
Forward (+)
45531572 .. 45532273
702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021280.1

Sequence Viewer

Length: 702 bp
ATGGCGAAGTTCTCGCTGAAGCCGAAGCGGACCAACCTCTACTTCCTGACTCTGGCCACAGTGCTCTGCACACTCTGCTACCTAATCGGCATCTGGCAGCACTCCTCCGGGACCCCCTCTTCCACCGCAGCCACGGCCTCAATCACCTCCGCCATCCCCTGCCCCCAGTTTTCCAAAAACATCACGACCCACCTCGACTTCGAGCCCCACCACCGCGCCGAGGACCTCACCATCCCTCCCGCGACGGCGCGTGTCACCCACGTGCCTCCATGCCACGCCAGCCTCTCCGAGTACACGCCCTGCGAGGACGCCACCCGGTCCTTGAAGTTCGACCGGGACCTGCTAATTTACCGGGAGCGGCATTGCCCGGAGAAGCAGGAGCTCCTCAAGTGCCGGATTCCGGCGCCGCACGGCTACACTGTGCCGTTCCGGTGGCCGGAGAGCCGGGGCGCTGTGTGGTACGCCAACGTTCCGCACAAGGAGTTGACTGTGGAGAAGAAAAACCAGAATTGGGTTCACTACAAGGGTGACCGGTTTCGATTCCCTGGGGGCGGCACCATGTTTCCTAACGGTGCGGACGCTTATATTGACGATATTGGCAAGTTGATTAATCTCAGAGACGGGTCTATTAGGACCGCCATTGATACCGGTTGTGGGGTAAGTATTACATTTTTCAATCTGATCCTCATTATTTTCAGGTAA

Protein Analysis

233

Amino Acids

26.28

Weight (kDa)

8.9

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 97 - 221 9.6e-52 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 348
AccB1I GGYRCC 2 cut(s) 403, 554
AccBSI CCGCTC 1 cut(s) 358
AccII CGCG 3 cut(s) 216, 242, 250
AclI AACGTT 1 cut(s) 468
AclWI GGATC 1 cut(s) 676
AcoI YGGCCR 2 cut(s) 54, 434
AcuI CTGAAG 1 cut(s) 38
AcvI CACGTG 1 cut(s) 262
AcyI GRCGYC 2 cut(s) 309, 404
AfaI GTAC 2 cut(s) 293, 461
AfiI CCNNNNNNNGG 7 cut(s) 52, 220, 400, 436, 511, 551, 654
AgeI ACCGGT 2 cut(s) 531, 647
AgsI TTSAA 2 cut(s) 325, 676
AjnI CCWGG 1 cut(s) 544
AjuI GAANNNNNNNTTGG 2 cut(s) 26, 58
AleI CACNNNNGTG 1 cut(s) 260
AluBI AGCT 1 cut(s) 382
AluI AGCT 1 cut(s) 382
Alw21I GWGCWC 2 cut(s) 66, 384
Alw26I GTCTC 1 cut(s) 612
AlwI GGATC 1 cut(s) 676
AoxI GGCC 3 cut(s) 54, 135, 434
ApeKI GCWGC 2 cut(s) 97, 128
AseI ATTAAT 1 cut(s) 609
AsiGI ACCGGT 2 cut(s) 531, 647
AspLEI GCGC 4 cut(s) 218, 250, 406, 452
AspS9I GGNCC 6 cut(s) 30, 111, 223, 318, 337, 633
AsuC2I CCSGG 6 cut(s) 109, 316, 335, 353, 368, 446
AsuHPI GGTGA 4 cut(s) 136, 220, 247, 539
AvaII GGWCC 6 cut(s) 30, 111, 223, 318, 337, 633
BalI TGGCCA 1 cut(s) 56
BanI GGYRCC 2 cut(s) 403, 554
BanII GRGCYC 2 cut(s) 207, 384
BbrPI CACGTG 1 cut(s) 262
Bbv12I GWGCWC 2 cut(s) 66, 384
BbvI GCAGC 2 cut(s) 109, 140
BccI CCATC 2 cut(s) 161, 239
BceAI ACGGC 4 cut(s) 150, 261, 409, 427
BciT130I CCWGG 1 cut(s) 546
BcnI CCSGG 6 cut(s) 109, 316, 335, 353, 368, 446
BcoDI GTCTC 1 cut(s) 612
BfoI RGCGCY 2 cut(s) 407, 453
BfuAI ACCTGC 1 cut(s) 348
BisI GCNGC 5 cut(s) 98, 129, 359, 407, 553
BlsI GCNGC 5 cut(s) 99, 130, 360, 408, 554
Bme1390I CCNGG 7 cut(s) 109, 316, 335, 353, 368, 446, 546
Bme18I GGWCC 6 cut(s) 30, 111, 223, 318, 337, 633
BmgT120I GGNCC 6 cut(s) 30, 111, 223, 318, 337, 633
BmiI GGNNCC 5 cut(s) 112, 113, 338, 405, 556
BmrFI CCNGG 7 cut(s) 109, 316, 335, 353, 368, 446, 546
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 1 cut(s) 99
BmuI ACTGGG 1 cut(s) 160
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 6 cut(s) 109, 316, 335, 353, 368, 446
BsaAI YACGTR 1 cut(s) 262
BsaHI GRCGYC 2 cut(s) 309, 404
BsaJI CCNNGG 5 cut(s) 132, 219, 445, 544, 545
BsaWI WCCGGW 3 cut(s) 429, 531, 647
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 7 cut(s) 52, 220, 400, 436, 511, 551, 654
Bse118I RCCGGY 2 cut(s) 531, 647
Bse1I ACTGG 1 cut(s) 166
Bse3DI GCAATG 1 cut(s) 361
BseBI CCWGG 1 cut(s) 546
BseDI CCNNGG 5 cut(s) 132, 219, 445, 544, 545
BseGI GGATG 2 cut(s) 153, 231
BseLI CCNNNNNNNGG 7 cut(s) 52, 220, 400, 436, 511, 551, 654
BseMI GCAATG 1 cut(s) 361
BseMII CTCAG 1 cut(s) 628
BseNI ACTGG 1 cut(s) 166
BseRI GAGGAG 2 cut(s) 94, 374
BseXI GCAGC 2 cut(s) 109, 140
BsgI GTGCAG 1 cut(s) 52
Bsh1236I CGCG 3 cut(s) 216, 242, 250
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 3 cut(s) 56, 137, 436
BshNI GGYRCC 2 cut(s) 403, 554
BshTI ACCGGT 2 cut(s) 531, 647
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 2 cut(s) 66, 384
BslFI GGGAC 2 cut(s) 124, 350
BslI CCNNNNNNNGG 7 cut(s) 52, 220, 400, 436, 511, 551, 654
BsmAI GTCTC 1 cut(s) 612
BsmBI CGTCTC 1 cut(s) 612
BsmFI GGGAC 2 cut(s) 124, 350
BsnI GGCC 3 cut(s) 56, 137, 436
Bsp1286I GDGCHC 3 cut(s) 66, 207, 384
Bsp143I GATC 1 cut(s) 681
BspANI GGCC 3 cut(s) 56, 137, 436
BspCNI CTCAG 1 cut(s) 627
BspFNI CGCG 3 cut(s) 216, 242, 250
BspLI GGNNCC 5 cut(s) 112, 113, 338, 405, 556
BspMI ACCTGC 1 cut(s) 348
BspPI GGATC 1 cut(s) 676
BspT107I GGYRCC 2 cut(s) 403, 554
BsrBI CCGCTC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 361
BsrFI RCCGGY 2 cut(s) 531, 647
BsrI ACTGG 1 cut(s) 166
BssAI RCCGGY 2 cut(s) 531, 647
BssECI CCNNGG 5 cut(s) 132, 219, 445, 544, 545
BssMI GATC 1 cut(s) 681
BssNI GRCGYC 2 cut(s) 309, 404
Bst2UI CCWGG 1 cut(s) 546
Bst4CI ACNGT 4 cut(s) 61, 421, 490, 572
Bst6I CTCTTC 1 cut(s) 124
BstACI GRCGYC 2 cut(s) 309, 404
BstAPI GCANNNNNTGC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 280
BstDEI CTNAG 1 cut(s) 614
BstDSI CCRYGG 1 cut(s) 132
BstEII GGTNACC 1 cut(s) 527
BstF5I GGATG 2 cut(s) 153, 231
BstFNI CGCG 3 cut(s) 216, 242, 250
BstH2I RGCGCY 2 cut(s) 407, 453
BstHHI GCGC 4 cut(s) 218, 250, 406, 452
BstKTI GATC 1 cut(s) 684
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 1 cut(s) 681
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 3 cut(s) 75, 134, 279
BstNI CCWGG 1 cut(s) 546
BstPI GGTNACC 1 cut(s) 527
BstSCI CCNGG 7 cut(s) 107, 314, 333, 351, 366, 444, 544
BstUI CGCG 3 cut(s) 216, 242, 250
BstV1I GCAGC 2 cut(s) 109, 140
BsuRI GGCC 3 cut(s) 56, 137, 436
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 2 cut(s) 153, 231
BtsIMutI CAGTG 2 cut(s) 66, 417
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 1 cut(s) 280
CfoI GCGC 4 cut(s) 218, 250, 406, 452
Cfr10I RCCGGY 2 cut(s) 531, 647
Cfr13I GGNCC 6 cut(s) 30, 111, 223, 318, 337, 633
CseI GACGC 2 cut(s) 317, 587
Csp6I GTAC 2 cut(s) 292, 460
CspAI ACCGGT 2 cut(s) 531, 647
CviAII CATG 2 cut(s) 270, 559
CviQI GTAC 2 cut(s) 292, 460
DdeI CTNAG 1 cut(s) 614
DinI GGCGCC 1 cut(s) 405
DpnI GATC 1 cut(s) 683
DpnII GATC 1 cut(s) 681
EaeI YGGCCR 2 cut(s) 54, 434
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
EciI GGCGGA 1 cut(s) 139
Ecl136II GAGCTC 1 cut(s) 382
Eco24I GRGCYC 2 cut(s) 207, 384
Eco47I GGWCC 6 cut(s) 30, 111, 223, 318, 337, 633
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 38
Eco72I CACGTG 1 cut(s) 262
Eco91I GGTNACC 1 cut(s) 527
EcoICRI GAGCTC 1 cut(s) 382
EcoO109I RGGNCCY 3 cut(s) 111, 223, 337
EcoO65I GGTNACC 1 cut(s) 527
EcoRII CCWGG 1 cut(s) 544
EcoT38I GRGCYC 2 cut(s) 207, 384
EgeI GGCGCC 1 cut(s) 405
EheI GGCGCC 1 cut(s) 405
Esp3I CGTCTC 1 cut(s) 612
FaeI CATG 2 cut(s) 273, 562
FaiI YATR 3 cut(s) 271, 560, 585
FaqI GGGAC 2 cut(s) 124, 350
FatI CATG 2 cut(s) 269, 558
FauI CCCGC 1 cut(s) 247
Fnu4HI GCNGC 5 cut(s) 98, 129, 359, 407, 553
FokI GGATG 2 cut(s) 140, 218
FriOI GRGCYC 2 cut(s) 207, 384
Fsp4HI GCNGC 5 cut(s) 98, 129, 359, 407, 553
GlaI GCGC 4 cut(s) 217, 249, 405, 451
GluI GCNGC 5 cut(s) 98, 129, 359, 407, 553
HaeII RGCGCY 2 cut(s) 407, 453
HaeIII GGCC 3 cut(s) 56, 137, 436
HgaI GACGC 2 cut(s) 317, 587
HhaI GCGC 4 cut(s) 218, 250, 406, 452
Hin1I GRCGYC 2 cut(s) 309, 404
Hin1II CATG 2 cut(s) 273, 562
Hin6I GCGC 4 cut(s) 216, 248, 404, 450
HinP1I GCGC 4 cut(s) 216, 248, 404, 450
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HinfI GANTC 3 cut(s) 49, 397, 540
HphI GGTGA 4 cut(s) 136, 220, 247, 539
Hpy166II GTNNAC 3 cut(s) 294, 486, 517
Hpy188I TCNGA 3 cut(s) 289, 617, 681
Hpy188III TCNNGA 2 cut(s) 46, 184
Hpy8I GTNNAC 3 cut(s) 294, 486, 517
Hpy99I CGWCG 1 cut(s) 247
HpyCH4III ACNGT 4 cut(s) 61, 421, 490, 572
HpyCH4IV ACGT 2 cut(s) 261, 468
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 134, 279
HpyF3I CTNAG 1 cut(s) 614
HpySE526I ACGT 2 cut(s) 261, 468
Hsp92I GRCGYC 2 cut(s) 309, 404
Hsp92II CATG 2 cut(s) 273, 562
HspAI GCGC 4 cut(s) 216, 248, 404, 450
KasI GGCGCC 1 cut(s) 403
KflI GGGWCCC 1 cut(s) 111
Kzo9I GATC 1 cut(s) 681
LmnI GCTCC 3 cut(s) 355, 379, 387
Lsp1109I GCAGC 2 cut(s) 109, 140
LweI GCATC 1 cut(s) 99
MaeII ACGT 2 cut(s) 261, 468
MaeIII GTNAC 2 cut(s) 253, 527
MalI GATC 1 cut(s) 683
MbiI CCGCTC 1 cut(s) 358
MboI GATC 1 cut(s) 681
MboII GAAGA 2 cut(s) 111, 508
MhlI GDGCHC 3 cut(s) 66, 207, 384
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 2 cut(s) 345, 508
MluNI TGGCCA 1 cut(s) 56
Mly113I GGCGCC 1 cut(s) 404
MlyI GAGTC 1 cut(s) 43
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 56
MspR9I CCNGG 7 cut(s) 109, 316, 335, 353, 368, 446, 546
MvaI CCWGG 1 cut(s) 546
MvnI CGCG 3 cut(s) 216, 242, 250
MwoI GCNNNNNNNGC 3 cut(s) 75, 134, 279
NarI GGCGCC 1 cut(s) 404
NciI CCSGG 6 cut(s) 109, 316, 335, 353, 368, 446
NdeII GATC 1 cut(s) 681
NlaIII CATG 2 cut(s) 273, 562
NlaIV GGNNCC 5 cut(s) 112, 113, 338, 405, 556
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 2 cut(s) 253, 527
OliI CACNNNNGTG 1 cut(s) 260
PasI CCCWGGG 1 cut(s) 545
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PfeI GAWTC 2 cut(s) 397, 540
PfoI TCCNGGA 1 cut(s) 107
PinAI ACCGGT 2 cut(s) 531, 647
PkrI GCNGC 5 cut(s) 99, 130, 360, 408, 554
PleI GAGTC 1 cut(s) 43
PluTI GGCGCC 1 cut(s) 407
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 1 cut(s) 43
Ppu21I YACGTR 1 cut(s) 262
PpuMI RGGWCCY 3 cut(s) 111, 223, 337
PshBI ATTAAT 1 cut(s) 609
Psp124BI GAGCTC 1 cut(s) 384
Psp1406I AACGTT 1 cut(s) 468
Psp5II RGGWCCY 3 cut(s) 111, 223, 337
Psp6I CCWGG 1 cut(s) 544
PspCI CACGTG 1 cut(s) 262
PspEI GGTNACC 1 cut(s) 527
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 5 cut(s) 112, 113, 338, 405, 556
PspPI GGNCC 6 cut(s) 30, 111, 223, 318, 337, 633
PspPPI RGGWCCY 3 cut(s) 111, 223, 337
RsaI GTAC 2 cut(s) 293, 461
RsaNI GTAC 2 cut(s) 292, 460
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 384
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 5 cut(s) 98, 129, 359, 407, 553
Sau3AI GATC 1 cut(s) 681
Sau96I GGNCC 6 cut(s) 30, 111, 223, 318, 337, 633
SchI GAGTC 1 cut(s) 43
ScrFI CCNGG 7 cut(s) 109, 316, 335, 353, 368, 446, 546
SduI GDGCHC 3 cut(s) 66, 207, 384
SfaNI GCATC 1 cut(s) 99
SfoI GGCGCC 1 cut(s) 405
SinI GGWCC 6 cut(s) 30, 111, 223, 318, 337, 633
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 2 cut(s) 345, 508
SspDI GGCGCC 1 cut(s) 403
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 7 cut(s) 107, 314, 333, 351, 366, 444, 544
TaaI ACNGT 4 cut(s) 61, 421, 490, 572
TaiI ACGT 2 cut(s) 264, 471
TaqI TCGA 4 cut(s) 195, 201, 330, 538
TasI AATT 2 cut(s) 345, 508
TatI WGTACW 1 cut(s) 291
TauI GCSGC 3 cut(s) 361, 409, 555
TfiI GAWTC 2 cut(s) 397, 540
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 2 cut(s) 66, 424
TseFI GTSAC 2 cut(s) 253, 527
TseI GCWGC 2 cut(s) 97, 128
Tsp45I GTSAC 2 cut(s) 253, 527
TspRI CASTG 2 cut(s) 66, 424
VpaK11BI GGWCC 6 cut(s) 30, 111, 223, 318, 337, 633
VspI ATTAAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.