Rh1BG214100

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0009758
Physical Location & Seq
Forward (+)
33417499 .. 33421343
3845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG214100.1

Sequence Viewer

Length: 1887 bp
ATGGCGAAGTTCTCGCTGAAGCCGAAGCGGACCAACCTCTACTTCCTGACTCTGGCCACAGTGCTCTGCACACTCTGCTACCTAATCGGCGTCTGGCAGCACTCCGCCAGGACCCCCTCCTCCACCGCCGCCACGACCTCCGTCACCTCCGCCGTCTCCTGCCCCCAGTTTTCTAAAAACATCACGACCCACCTCGACTTCAAGCCCCACCACCGCGCCGAGGACCTCCCCGTCCCTCCCGCGACGGCGCGTGTCACCCACGTGCCGCCATGCGACGCCAGCCTCTCCGAGTACACGCCCTGCGAGGACGCCACCCGGTCCTTGAAGTTCGACCGGGACCGGCTAATTTACCGGGAGCGGCATTGCCCGGAGAAGCAGGAGCTCCTCAAGTGCAGGATTCCGGCGCCGCACGGCTACACCGTGCCGTTCCGGTGGCCGGAGAGCCGGGGCGCGGTGTGGTACGCCAACGTTCCGCACAAGGAGTTGACTGTGGAGAAGAAAAACCAGAATTGGGTTCACTACGAGGGTGACCGGTTTCGATTCCCTGGGGGCGGGACCATGTTTCCTAACGGTGCGGACGCGTATATTGACGATATTGGCAAGTTGATTAATCTCAGAGACGGGTCTATTAGGACCGCCATTGATACCGGTTGTGGGGTTGCAAGTTGGGGTGCTTACCTTTTGTCTAGGGACATTTTAACAGTGTCATTTGCACCAAGAGACACTCATGAAGCACAAGTTCAATTTGCTCTTGAAAGAGGAGTTCCTGCAGTGATTGGTGTAATGGCTTCAATTAGACTTCCCTATCCCTCGAGAGCTTTCGACATGGCTCACTGCTCTCGTTGTCTCATTCCGTGGGGCCAATATGATGGGCTGTACTTGATTGAAGTGGATCGTGTTTTACGACCCGGGGGGTATTGGATTCTGTCCGGCCCACCAATCAACTGGCAGAATCACTGGAAAGGTTGGCAAAGAACTGAGGCAGACCTCAAAGCTGAGCAGGACATGATTGAGAGAGTAGCAAAGAGCTTATGCTGGAAAAAATTGAAGCAGAAGGGTGACCTTGCCATTTGGAAAAAACCCACTAACCATATCCACTGTCAAGCCAACAGAAAGGTCTTCAAGAAACCATCCTTCTGCCAAGCTCAAAATCCAGACACAGCATGGTATACTAAACTTGAGGAGTGTTTGACTCCATTGCCCGGAGTGAACAACATAAAAGAAGTTGCAGGTGGGGAATTGGCTAAATGGCCAGCGAGGCTAACTACAGTTCCTCCAAGAATTAGTAGTGGAAGTTTGAAAGGAATTACAGCTGAGATGTTCAGAGAAAACACGGCGCTATGGAAACAGAGAGTTGCACATTATAAGACCTTGGACTATCAGTTGGCAGACCCTGGGAGGTATCGTAATTTGCTTGACATGAACTCTTACTTGGGGAGCTTTGCGGCTGCACTGGCTGATGACCCTGCATGGGTTATGAACATTGTCCCGGTTGAGGCTGAGGTGAACACTCTTGGAGCCATTTATGAACGTGGATTGATCGGAACTTATCAGAATTGGTGTGAGGCCATGTCTACTTACCCAAGAACCTATGACTTCATCCACTCTGATTCGGTTTTCACTCTTTACAAGGACAGATGCGAAATGGAAGACATTCTTCTAGAAATGGATAGGATTTTAAGGCCACAAGGCAGTACAATTTTCAGGGATGATGTGGATATGGTTCTGAAGGCCAAGGCACTCTTAGAAGCAATGCAATATGATGCCAGAATTGTAGACCATGAAGAGGGTCCTCATCATAGAGAAAAGATATTGTTGGCGGCTAAGCAGTACTGGACAGCACCAGCACCAAACCAAAAAGAAGGAGAAAGCAGTAAAGAATCATAG

Protein Analysis

628

Amino Acids

71.29

Weight (kDa)

8.44

Isoelectric Point (pI)

34.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 97 - 609 7.5e-223 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1365
AarI CACCTGC 1 cut(s) 1220
AasI GACNNNNNNGTC 1 cut(s) 230
Acc36I ACCTGC 1 cut(s) 1220
AccB1I GGYRCC 1 cut(s) 403
AccBSI CCGCTC 1 cut(s) 358
AccI GTMKAC 3 cut(s) 1169, 1574, 1776
AccII CGCG 5 cut(s) 216, 242, 250, 452, 581
AclI AACGTT 1 cut(s) 468
AclWI GGATC 1 cut(s) 900
AcoI YGGCCR 3 cut(s) 54, 434, 1250
AcuI CTGAAG 2 cut(s) 38, 1748
AcvI CACGTG 1 cut(s) 262
AcyI GRCGYC 4 cut(s) 90, 276, 309, 404
AfaI GTAC 5 cut(s) 293, 461, 878, 1696, 1832
AflIII ACRYGT 1 cut(s) 579
AgeI ACCGGT 2 cut(s) 531, 647
AgsI TTSAA 9 cut(s) 202, 325, 743, 755, 792, 887, 1048, 1123, 1300
AjnI CCWGG 3 cut(s) 107, 544, 1393
AjuI GAANNNNNNNTTGG 4 cut(s) 26, 58, 1415, 1447
AleI CACNNNNGTG 1 cut(s) 260
AluBI AGCT 7 cut(s) 382, 818, 995, 1029, 1145, 1313, 1440
AluI AGCT 7 cut(s) 382, 818, 995, 1029, 1145, 1313, 1440
Alw21I GWGCWC 2 cut(s) 66, 384
Alw26I GTCTC 4 cut(s) 160, 612, 714, 851
AlwI GGATC 1 cut(s) 900
AlwNI CAGNNNCTG 1 cut(s) 1394
Ama87I CYCGRG 2 cut(s) 811, 908
AoxI GGCC 8 cut(s) 54, 434, 859, 931, 1250, 1566, 1682, 1731
ApeKI GCWGC 2 cut(s) 97, 1448
AseI ATTAAT 1 cut(s) 609
AsiGI ACCGGT 2 cut(s) 531, 647
Asp700I GAANNNNTTC 1 cut(s) 1653
AspLEI GCGC 5 cut(s) 218, 250, 406, 452, 1339
AsuC2I CCSGG 9 cut(s) 316, 335, 353, 368, 446, 909, 910, 1203, 1490
AsuHPI GGTGA 5 cut(s) 136, 247, 539, 1070, 1516
AvaI CYCGRG 2 cut(s) 811, 908
AvaII GGWCC 8 cut(s) 30, 111, 223, 318, 337, 555, 633, 1790
BalI TGGCCA 2 cut(s) 56, 1252
BanI GGYRCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 384
BbrPI CACGTG 1 cut(s) 262
BbsI GAAGAC 2 cut(s) 1111, 1656
Bbv12I GWGCWC 2 cut(s) 66, 384
BbvCI CCTCAGC 1 cut(s) 1500
BbvI GCAGC 2 cut(s) 109, 1435
BccI CCATC 2 cut(s) 863, 1138
BceAI ACGGC 5 cut(s) 137, 261, 409, 427, 1350
BciT130I CCWGG 3 cut(s) 109, 546, 1395
BcnI CCSGG 9 cut(s) 316, 335, 353, 368, 446, 909, 910, 1203, 1490
BcoDI GTCTC 4 cut(s) 160, 612, 714, 851
BfaI CTAG 2 cut(s) 687, 1661
BfmI CTRYAG 2 cut(s) 768, 1266
BfoI RGCGCY 2 cut(s) 407, 1340
BfuAI ACCTGC 1 cut(s) 1220
BglI GCCNNNNNGGC 1 cut(s) 1258
BisI GCNGC 8 cut(s) 98, 129, 266, 359, 407, 1446, 1449, 1821
BlpI GCTNAGC 2 cut(s) 996, 1824
BlsI GCNGC 8 cut(s) 99, 130, 267, 360, 408, 1447, 1450, 1822
BmcAI AGTACT 1 cut(s) 1832
Bme18I GGWCC 8 cut(s) 30, 111, 223, 318, 337, 555, 633, 1790
BmeT110I CYCGRG 2 cut(s) 811, 908
BmiI GGNNCC 7 cut(s) 113, 338, 405, 556, 860, 1519, 1791
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 2 cut(s) 1628, 1753
BmuI ACTGGG 1 cut(s) 160
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 1111, 1656
Bpu10I CCTNAGC 1 cut(s) 1500
Bpu1102I GCTNAGC 2 cut(s) 996, 1824
BpuEI CTTGAG 2 cut(s) 371, 1199
BpuMI CCSGG 9 cut(s) 316, 335, 353, 368, 446, 909, 910, 1203, 1490
BsaAI YACGTR 1 cut(s) 262
BsaHI GRCGYC 4 cut(s) 90, 276, 309, 404
BsaWI WCCGGW 3 cut(s) 429, 531, 647
BsaXI ACNNNNNCTCC 4 cut(s) 1258, 1288, 1390, 1420
Bse118I RCCGGY 3 cut(s) 339, 531, 647
Bse1I ACTGG 5 cut(s) 166, 950, 962, 1458, 1838
Bse3DI GCAATG 3 cut(s) 361, 1196, 1758
BseBI CCWGG 3 cut(s) 109, 546, 1395
BseGI GGATG 3 cut(s) 1130, 1599, 1714
BseMI GCAATG 3 cut(s) 361, 1196, 1758
BseMII CTCAG 5 cut(s) 628, 969, 987, 1305, 1491
BseNI ACTGG 5 cut(s) 166, 950, 962, 1458, 1838
BseRI GAGGAG 4 cut(s) 109, 374, 774, 1196
BseXI GCAGC 2 cut(s) 109, 1435
BsgI GTGCAG 3 cut(s) 52, 412, 1434
Bsh1236I CGCG 5 cut(s) 216, 242, 250, 452, 581
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 8 cut(s) 56, 436, 861, 933, 1252, 1568, 1684, 1733
BshNI GGYRCC 1 cut(s) 403
BshTI ACCGGT 2 cut(s) 531, 647
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 2 cut(s) 66, 384
BsiHKCI CYCGRG 2 cut(s) 811, 908
BslFI GGGAC 5 cut(s) 218, 350, 568, 704, 1472
BsmAI GTCTC 4 cut(s) 160, 612, 714, 851
BsmBI CGTCTC 2 cut(s) 160, 612
BsmFI GGGAC 5 cut(s) 218, 350, 568, 704, 1472
BsnI GGCC 8 cut(s) 56, 436, 861, 933, 1252, 1568, 1684, 1733
BsoBI CYCGRG 2 cut(s) 811, 908
Bsp1286I GDGCHC 2 cut(s) 66, 384
Bsp143I GATC 2 cut(s) 892, 1539
Bsp1720I GCTNAGC 2 cut(s) 996, 1824
BspANI GGCC 8 cut(s) 56, 436, 861, 933, 1252, 1568, 1684, 1733
BspCNI CTCAG 5 cut(s) 627, 970, 988, 1306, 1492
BspFNI CGCG 5 cut(s) 216, 242, 250, 452, 581
BspHI TCATGA 1 cut(s) 727
BspLI GGNNCC 7 cut(s) 113, 338, 405, 556, 860, 1519, 1791
BspMAI CTGCAG 1 cut(s) 772
BspMI ACCTGC 1 cut(s) 1220
BspPI GGATC 1 cut(s) 900
BspT107I GGYRCC 1 cut(s) 403
BsrBI CCGCTC 1 cut(s) 358
BsrDI GCAATG 3 cut(s) 361, 1196, 1758
BsrFI RCCGGY 3 cut(s) 339, 531, 647
BsrI ACTGG 5 cut(s) 166, 950, 962, 1458, 1838
BssAI RCCGGY 3 cut(s) 339, 531, 647
BssMI GATC 2 cut(s) 892, 1539
BssNAI GTATAC 1 cut(s) 1170
BssNI GRCGYC 4 cut(s) 90, 276, 309, 404
BssT1I CCWWGG 2 cut(s) 1371, 1734
Bst1107I GTATAC 1 cut(s) 1170
Bst2UI CCWGG 3 cut(s) 109, 546, 1395
Bst4CI ACNGT 7 cut(s) 61, 421, 490, 572, 703, 1100, 1270
Bst6I CTCTTC 1 cut(s) 1779
BstACI GRCGYC 4 cut(s) 90, 276, 309, 404
BstAPI GCANNNNNTGC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 262
BstC8I GCNNGC 2 cut(s) 280, 1254
BstDEI CTNAG 7 cut(s) 614, 978, 996, 1314, 1500, 1744, 1824
BstDSI CCRYGG 1 cut(s) 854
BstEII GGTNACC 2 cut(s) 527, 1058
BstF5I GGATG 3 cut(s) 1130, 1599, 1714
BstFNI CGCG 5 cut(s) 216, 242, 250, 452, 581
BstH2I RGCGCY 2 cut(s) 407, 1340
BstHHI GCGC 5 cut(s) 218, 250, 406, 452, 1339
BstKTI GATC 2 cut(s) 895, 1542
BstMAI GTCTC 4 cut(s) 160, 612, 714, 851
BstMBI GATC 2 cut(s) 892, 1539
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 4 cut(s) 75, 279, 1258, 1454
BstNI CCWGG 3 cut(s) 109, 546, 1395
BstPAI GACNNNNGTC 1 cut(s) 140
BstPI GGTNACC 2 cut(s) 527, 1058
BstSFI CTRYAG 2 cut(s) 768, 1266
BstUI CGCG 5 cut(s) 216, 242, 250, 452, 581
BstV1I GCAGC 2 cut(s) 109, 1435
BstV2I GAAGAC 2 cut(s) 1111, 1656
BstXI CCANNNNNNTGG 2 cut(s) 869, 945
BstZ17I GTATAC 1 cut(s) 1170
BsuRI GGCC 8 cut(s) 56, 436, 861, 933, 1252, 1568, 1684, 1733
BtgI CCRYGG 1 cut(s) 854
BtsCI GGATG 3 cut(s) 1130, 1599, 1714
BtsI GCAGTG 2 cut(s) 777, 832
BtsIMutI CAGTG 7 cut(s) 66, 708, 777, 832, 955, 1096, 1451
BveI ACCTGC 1 cut(s) 1220
Cac8I GCNNGC 2 cut(s) 280, 1254
CaiI CAGNNNCTG 1 cut(s) 1394
CciI TCATGA 1 cut(s) 727
CfoI GCGC 5 cut(s) 218, 250, 406, 452, 1339
Cfr10I RCCGGY 3 cut(s) 339, 531, 647
Cfr9I CCCGGG 1 cut(s) 908
CseI GACGC 4 cut(s) 79, 284, 317, 587
Csp6I GTAC 5 cut(s) 292, 460, 877, 1695, 1831
CspAI ACCGGT 2 cut(s) 531, 647
CviQI GTAC 5 cut(s) 292, 460, 877, 1695, 1831
DdeI CTNAG 7 cut(s) 614, 978, 996, 1314, 1500, 1744, 1824
DinI GGCGCC 1 cut(s) 405
DpnI GATC 2 cut(s) 894, 1541
DpnII GATC 2 cut(s) 892, 1539
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
EaeI YGGCCR 3 cut(s) 54, 434, 1250
Eam1104I CTCTTC 1 cut(s) 1779
EarI CTCTTC 1 cut(s) 1779
EciI GGCGGA 2 cut(s) 94, 139
Ecl136II GAGCTC 1 cut(s) 382
Eco130I CCWWGG 2 cut(s) 1371, 1734
Eco24I GRGCYC 1 cut(s) 384
Eco47I GGWCC 8 cut(s) 30, 111, 223, 318, 337, 555, 633, 1790
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 2 cut(s) 38, 1748
Eco72I CACGTG 1 cut(s) 262
Eco88I CYCGRG 2 cut(s) 811, 908
Eco91I GGTNACC 2 cut(s) 527, 1058
EcoICRI GAGCTC 1 cut(s) 382
EcoO109I RGGNCCY 3 cut(s) 111, 223, 1790
EcoO65I GGTNACC 2 cut(s) 527, 1058
EcoRII CCWGG 3 cut(s) 107, 544, 1393
EcoT14I CCWWGG 2 cut(s) 1371, 1734
EcoT38I GRGCYC 1 cut(s) 384
EgeI GGCGCC 1 cut(s) 405
EheI GGCGCC 1 cut(s) 405
ErhI CCWWGG 2 cut(s) 1371, 1734
Esp3I CGTCTC 2 cut(s) 160, 612
FalI AAGNNNNNCTT 4 cut(s) 1641, 1673, 1727, 1759
FaqI GGGAC 5 cut(s) 218, 350, 568, 704, 1472
FauI CCCGC 2 cut(s) 247, 545
FblI GTMKAC 3 cut(s) 1169, 1574, 1776
Fnu4HI GCNGC 8 cut(s) 98, 129, 266, 359, 407, 1446, 1449, 1821
FokI GGATG 3 cut(s) 1117, 1586, 1721
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 8 cut(s) 98, 129, 266, 359, 407, 1446, 1449, 1821
FspBI CTAG 2 cut(s) 687, 1661
GlaI GCGC 5 cut(s) 217, 249, 405, 451, 1338
GluI GCNGC 8 cut(s) 98, 129, 266, 359, 407, 1446, 1449, 1821
HaeII RGCGCY 2 cut(s) 407, 1340
HaeIII GGCC 8 cut(s) 56, 436, 861, 933, 1252, 1568, 1684, 1733
HgaI GACGC 4 cut(s) 79, 284, 317, 587
HhaI GCGC 5 cut(s) 218, 250, 406, 452, 1339
Hin1I GRCGYC 4 cut(s) 90, 276, 309, 404
Hin6I GCGC 5 cut(s) 216, 248, 404, 450, 1337
HinP1I GCGC 5 cut(s) 216, 248, 404, 450, 1337
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HinfI GANTC 8 cut(s) 49, 397, 540, 922, 952, 1192, 1610, 1880
HphI GGTGA 5 cut(s) 136, 247, 539, 1070, 1516
Hpy166II GTNNAC 8 cut(s) 294, 486, 517, 1170, 1210, 1507, 1575, 1777
Hpy188I TCNGA 7 cut(s) 289, 617, 1325, 1544, 1554, 1609, 1728
Hpy188III TCNNGA 8 cut(s) 46, 184, 728, 752, 813, 1123, 1154, 1661
Hpy8I GTNNAC 8 cut(s) 294, 486, 517, 1170, 1210, 1507, 1575, 1777
Hpy99I CGWCG 2 cut(s) 247, 278
HpyAV CCTTC 4 cut(s) 1048, 1144, 1723, 1856
HpyCH4III ACNGT 7 cut(s) 61, 421, 490, 572, 703, 1100, 1270
HpyCH4IV ACGT 3 cut(s) 261, 468, 1531
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 279, 1258, 1454
HpyF3I CTNAG 7 cut(s) 614, 978, 996, 1314, 1500, 1744, 1824
HpySE526I ACGT 3 cut(s) 261, 468, 1531
Hsp92I GRCGYC 4 cut(s) 90, 276, 309, 404
HspAI GCGC 5 cut(s) 216, 248, 404, 450, 1337
KasI GGCGCC 1 cut(s) 403
Kzo9I GATC 2 cut(s) 892, 1539
LmnI GCTCC 5 cut(s) 355, 379, 387, 1437, 1517
Lsp1109I GCAGC 2 cut(s) 109, 1435
LweI GCATC 2 cut(s) 1628, 1753
MaeI CTAG 2 cut(s) 687, 1661
MaeII ACGT 3 cut(s) 261, 468, 1531
MaeIII GTNAC 4 cut(s) 142, 253, 527, 1058
MalI GATC 2 cut(s) 894, 1541
MbiI CCGCTC 1 cut(s) 358
MboI GATC 2 cut(s) 892, 1539
MboII GAAGA 5 cut(s) 508, 1111, 1649, 1661, 1796
MhlI GDGCHC 2 cut(s) 66, 384
MlsI TGGCCA 2 cut(s) 56, 1252
MluI ACGCGT 1 cut(s) 579
MluNI TGGCCA 2 cut(s) 56, 1252
Mly113I GGCGCC 1 cut(s) 404
MlyI GAGTC 2 cut(s) 43, 1186
Mox20I TGGCCA 2 cut(s) 56, 1252
MroXI GAANNNNTTC 1 cut(s) 1653
MscI TGGCCA 2 cut(s) 56, 1252
MseI TTAA 3 cut(s) 609, 698, 1679
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 2 cut(s) 56, 1252
MspA1I CMGCKG 1 cut(s) 1313
MvaI CCWGG 3 cut(s) 109, 546, 1395
MvnI CGCG 5 cut(s) 216, 242, 250, 452, 581
MwoI GCNNNNNNNGC 4 cut(s) 75, 279, 1258, 1454
NarI GGCGCC 1 cut(s) 404
NciI CCSGG 9 cut(s) 316, 335, 353, 368, 446, 909, 910, 1203, 1490
NdeII GATC 2 cut(s) 892, 1539
NlaIV GGNNCC 7 cut(s) 113, 338, 405, 556, 860, 1519, 1791
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 4 cut(s) 142, 253, 527, 1058
OliI CACNNNNGTG 1 cut(s) 260
PaeR7I CTCGAG 1 cut(s) 811
PagI TCATGA 1 cut(s) 727
PaqCI CACCTGC 1 cut(s) 1220
PasI CCCWGGG 2 cut(s) 545, 1394
PcsI WCGNNNNNNNCGW 3 cut(s) 20, 417, 901
PdmI GAANNNNTTC 1 cut(s) 1653
PfeI GAWTC 6 cut(s) 397, 540, 922, 952, 1610, 1880
PinAI ACCGGT 2 cut(s) 531, 647
PkrI GCNGC 8 cut(s) 99, 130, 267, 360, 408, 1447, 1450, 1822
PleI GAGTC 2 cut(s) 43, 1186
PluTI GGCGCC 1 cut(s) 407
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 2 cut(s) 43, 1186
Ppu21I YACGTR 1 cut(s) 262
PpuMI RGGWCCY 3 cut(s) 111, 223, 1790
PshAI GACNNNNGTC 1 cut(s) 140
PshBI ATTAAT 1 cut(s) 609
PsiI TTATAA 1 cut(s) 1365
Psp124BI GAGCTC 1 cut(s) 384
Psp1406I AACGTT 1 cut(s) 468
Psp5II RGGWCCY 3 cut(s) 111, 223, 1790
Psp6I CCWGG 3 cut(s) 107, 544, 1393
PspCI CACGTG 1 cut(s) 262
PspEI GGTNACC 2 cut(s) 527, 1058
PspGI CCWGG 3 cut(s) 107, 544, 1393
PspN4I GGNNCC 7 cut(s) 113, 338, 405, 556, 860, 1519, 1791
PspPPI RGGWCCY 3 cut(s) 111, 223, 1790
PstI CTGCAG 1 cut(s) 772
PstNI CAGNNNCTG 1 cut(s) 1394
PvuII CAGCTG 1 cut(s) 1313
RsaI GTAC 5 cut(s) 293, 461, 878, 1696, 1832
RsaNI GTAC 5 cut(s) 292, 460, 877, 1695, 1831
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 384
SaqAI TTAA 3 cut(s) 609, 698, 1679
SatI GCNGC 8 cut(s) 98, 129, 266, 359, 407, 1446, 1449, 1821
Sau3AI GATC 2 cut(s) 892, 1539
ScaI AGTACT 1 cut(s) 1832
SchI GAGTC 2 cut(s) 43, 1186
SduI GDGCHC 2 cut(s) 66, 384
SfaNI GCATC 2 cut(s) 1628, 1753
SfcI CTRYAG 2 cut(s) 768, 1266
SfoI GGCGCC 1 cut(s) 405
Sfr274I CTCGAG 1 cut(s) 811
SinI GGWCC 8 cut(s) 30, 111, 223, 318, 337, 555, 633, 1790
SlaI CTCGAG 1 cut(s) 811
SmaI CCCGGG 1 cut(s) 910
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 3 cut(s) 386, 811, 1178
SmoI CTYRAG 3 cut(s) 386, 811, 1178
SspDI GGCGCC 1 cut(s) 403
SspMI CTAG 2 cut(s) 687, 1661
SstI GAGCTC 1 cut(s) 384
StyI CCWWGG 2 cut(s) 1371, 1734
TaaI ACNGT 7 cut(s) 61, 421, 490, 572, 703, 1100, 1270
TaiI ACGT 3 cut(s) 264, 471, 1534
TaqI TCGA 5 cut(s) 195, 330, 538, 812, 822
TatI WGTACW 4 cut(s) 291, 876, 1694, 1830
TauI GCSGC 6 cut(s) 131, 268, 361, 409, 1448, 1823
TfiI GAWTC 6 cut(s) 397, 540, 922, 952, 1610, 1880
Tru1I TTAA 3 cut(s) 609, 698, 1679
Tru9I TTAA 3 cut(s) 609, 698, 1679
TscAI CASTG 7 cut(s) 66, 708, 777, 839, 962, 1103, 1458
TseFI GTSAC 4 cut(s) 142, 253, 527, 1058
TseI GCWGC 2 cut(s) 97, 1448
Tsp45I GTSAC 4 cut(s) 142, 253, 527, 1058
TspDTI ATGAA 6 cut(s) 744, 1436, 1493, 1542, 1588, 1797
TspGWI ACGGA 2 cut(s) 130, 843
TspMI CCCGGG 1 cut(s) 908
TspRI CASTG 7 cut(s) 66, 708, 777, 839, 962, 1103, 1458
VpaK11BI GGWCC 8 cut(s) 30, 111, 223, 318, 337, 555, 633, 1790
VspI ATTAAT 1 cut(s) 609
XbaI TCTAGA 1 cut(s) 1660
XcmI CCANNNNNNNNNTGG 2 cut(s) 942, 1161
XhoI CTCGAG 1 cut(s) 811
XmaI CCCGGG 1 cut(s) 908
XmiI GTMKAC 3 cut(s) 1169, 1574, 1776
XmnI GAANNNNTTC 1 cut(s) 1653
XspI CTAG 2 cut(s) 687, 1661
ZrmI AGTACT 1 cut(s) 1832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.