pycom07g12340

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009758
Physical Location & Seq
Reverse (-)
13652022 .. 13655269
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g12340.1

Sequence Viewer

Length: 1050 bp
ATGCATTATACACACCTCAACTCTCTCTCACTCTCTCTCTCTAAAACAATGGCTTGTCCAAGTCCACTACAGTACTTTTCACTGAAACCCAAAAGAGCCAACCTTTATTACATGACGTTAGCCGCAGTCCTCTGCACGCTCTGCTACCTGGTCGGAATTTGGCAGCACTCATCCGGCGGCGCCGTCATCACCGCCCCTGTCTCCGCCGTCGCCTCATGTCCTCCAATCACCATAAACAAAACAATCCATCTCGACTTCAAAGCCCACCACACCGCTGACGACCTCCCCCTACCTCCCGCGGCGGCGCGTGTGGATCACCTGCCGCCTTGCAACGCCAAGCTCAGCGAGTACACACCGTGCGAGGACCTCACTAGGTCGCTGAAGTTCGACAGGAAGAGGTTGGTGTACAGGGAGAGGCATTGCCCGGAGAAGGAGGAGCTCCTCAAGTGCCGAATTCCGGCACCGCACGGGTACACGGTGCCGCTTCGGTGGCCGGAGAGCCGGGACTCGGTTTGGTATGCGAACGTGCCGCATAAGGAGTTGACCGTGGAGAAGAAGAAGCAAAATTGGGTTCATTACGACGGGGACCGGTTTAGATTCCCTGGTGGCGGGACCATGTTTCCTCGCGGTGCTGATGCTTATATCGACGACATCGGAAAGTTGATTAATCTCAGAGATGGGTCTGTACGGACCGCCATTGATACCGGTTGCGGGGTTGCAAGTTGGGGAGCTTACCTTTTGTCCAGGGACATCCTAACAGTGTCATTTGCACCAAGAGACACCCATGAAGCACAAGTTCAATTTGCTCTTGAAAGAGGAGTCCCTGCATTGATTGGCATTCTTGCTTCCAAAAGACTTCCTTACCCCTCTAGAGCTTTTGACATGGCTCACTGCTCTCGTTGCCTCATTCCCTGGGGCCAATATGATGGGCTTTACTTAATTGAAGTTGATCGAGTTTTACGCCCCGGGGGGTATTGGATTCTTTCCGGTCCACCAATAAACTGGGAGAATCACTGGAAAGGATGGGAAAGAACAGCTGAAGATCTTTGA

Protein Analysis

350

Amino Acids

39.37

Weight (kDa)

8.68

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 116 - 336 7.5e-114 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 327
Acc36I ACCTGC 1 cut(s) 327
AccB1I GGYRCC 3 cut(s) 179, 460, 478
AccII CGCG 3 cut(s) 299, 307, 627
AclWI GGATC 1 cut(s) 321
AcoI YGGCCR 1 cut(s) 491
AcsI RAATTY 2 cut(s) 156, 453
AcuI CTGAAG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 357
AfaI GTAC 5 cut(s) 74, 350, 407, 473, 687
AfiI CCNNNNNNNGG 5 cut(s) 430, 457, 508, 608, 711
AgeI ACCGGT 2 cut(s) 588, 704
AgsI TTSAA 4 cut(s) 259, 800, 812, 944
AjnI CCWGG 4 cut(s) 147, 601, 743, 911
AluBI AGCT 5 cut(s) 340, 439, 731, 875, 1037
AluI AGCT 5 cut(s) 340, 439, 731, 875, 1037
Alw21I GWGCWC 1 cut(s) 441
Alw26I GTCTC 2 cut(s) 205, 771
AlwI GGATC 1 cut(s) 321
Ama87I CYCGRG 1 cut(s) 965
AoxI GGCC 2 cut(s) 491, 916
ApeKI GCWGC 1 cut(s) 163
ApoI RAATTY 2 cut(s) 156, 453
AseI ATTAAT 1 cut(s) 666
AsiGI ACCGGT 2 cut(s) 588, 704
AspLEI GCGC 2 cut(s) 182, 307
AspS9I GGNCC 6 cut(s) 364, 586, 612, 690, 916, 989
AsuC2I CCSGG 4 cut(s) 425, 503, 966, 967
AsuHPI GGTGA 3 cut(s) 181, 220, 308
AvaI CYCGRG 1 cut(s) 965
AvaII GGWCC 5 cut(s) 364, 586, 612, 690, 989
BanI GGYRCC 3 cut(s) 179, 460, 478
BanII GRGCYC 1 cut(s) 441
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 175
BccI CCATC 4 cut(s) 255, 671, 920, 1017
BceAI ACGGC 2 cut(s) 167, 191
BciT130I CCWGG 4 cut(s) 149, 603, 745, 913
BcnI CCSGG 4 cut(s) 425, 503, 966, 967
BcoDI GTCTC 2 cut(s) 205, 771
BfaI CTAG 2 cut(s) 372, 870
BfmI CTRYAG 1 cut(s) 68
BfoI RGCGCY 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 327
BglII AGATCT 1 cut(s) 1042
BisI GCNGC 8 cut(s) 123, 164, 178, 300, 303, 323, 482, 530
BlpI GCTNAGC 1 cut(s) 341
BlsI GCNGC 8 cut(s) 124, 165, 179, 301, 304, 324, 483, 531
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 8 cut(s) 149, 425, 503, 603, 745, 913, 966, 967
Bme18I GGWCC 5 cut(s) 364, 586, 612, 690, 989
BmeT110I CYCGRG 1 cut(s) 965
BmgT120I GGNCC 6 cut(s) 364, 586, 612, 690, 916, 989
BmiI GGNNCC 6 cut(s) 181, 462, 480, 587, 613, 917
BmrFI CCNGG 8 cut(s) 149, 425, 503, 603, 745, 913, 966, 967
BmrI ACTGGG 1 cut(s) 1012
BmsI GCATC 1 cut(s) 625
BmuI ACTGGG 1 cut(s) 1012
Bpu1102I GCTNAGC 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 4 cut(s) 425, 503, 966, 967
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 9 cut(s) 297, 546, 601, 744, 911, 912, 964, 965, 966
BsaWI WCCGGW 3 cut(s) 588, 704, 986
Bsc4I CCNNNNNNNGG 5 cut(s) 430, 457, 508, 608, 711
Bse118I RCCGGY 2 cut(s) 588, 704
Bse1I ACTGG 2 cut(s) 1007, 1019
Bse3DI GCAATG 1 cut(s) 418
BseBI CCWGG 4 cut(s) 149, 603, 745, 913
BseDI CCNNGG 9 cut(s) 297, 546, 601, 744, 911, 912, 964, 965, 966
BseGI GGATG 3 cut(s) 170, 750, 1028
BseLI CCNNNNNNNGG 5 cut(s) 430, 457, 508, 608, 711
BseMI GCAATG 1 cut(s) 418
BseMII CTCAG 2 cut(s) 355, 685
BseNI ACTGG 2 cut(s) 1007, 1019
BseRI GAGGAG 3 cut(s) 431, 449, 831
BseXI GCAGC 1 cut(s) 175
BsgI GTGCAG 1 cut(s) 118
Bsh1236I CGCG 3 cut(s) 299, 307, 627
BshFI GGCC 2 cut(s) 493, 918
BshNI GGYRCC 3 cut(s) 179, 460, 478
BshTI ACCGGT 2 cut(s) 588, 704
BsiHKAI GWGCWC 1 cut(s) 441
BsiHKCI CYCGRG 1 cut(s) 965
BsiSI CCGG 9 cut(s) 174, 425, 458, 494, 502, 589, 705, 966, 987
BslFI GGGAC 5 cut(s) 518, 599, 625, 761, 806
BslI CCNNNNNNNGG 5 cut(s) 430, 457, 508, 608, 711
BsmAI GTCTC 2 cut(s) 205, 771
BsmFI GGGAC 5 cut(s) 518, 599, 625, 761, 806
BsmI GAATGC 1 cut(s) 837
BsnI GGCC 2 cut(s) 493, 918
BsoBI CYCGRG 1 cut(s) 965
Bsp1286I GDGCHC 1 cut(s) 441
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 3 cut(s) 313, 949, 1042
Bsp1720I GCTNAGC 1 cut(s) 341
BspANI GGCC 2 cut(s) 493, 918
BspCNI CTCAG 2 cut(s) 354, 684
BspFNI CGCG 3 cut(s) 299, 307, 627
BspLI GGNNCC 6 cut(s) 181, 462, 480, 587, 613, 917
BspMI ACCTGC 1 cut(s) 327
BspPI GGATC 1 cut(s) 321
BspT107I GGYRCC 3 cut(s) 179, 460, 478
BsrDI GCAATG 1 cut(s) 418
BsrFI RCCGGY 2 cut(s) 588, 704
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 2 cut(s) 1007, 1019
BssAI RCCGGY 2 cut(s) 588, 704
BssECI CCNNGG 9 cut(s) 297, 546, 601, 744, 911, 912, 964, 965, 966
BssMI GATC 3 cut(s) 313, 949, 1042
BssNI GRCGYC 1 cut(s) 180
Bst2UI CCWGG 4 cut(s) 149, 603, 745, 913
Bst4CI ACNGT 5 cut(s) 72, 357, 478, 547, 760
Bst6I CTCTTC 1 cut(s) 389
BstACI GRCGYC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 141
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 2 cut(s) 341, 671
BstDSI CCRYGG 2 cut(s) 297, 546
BstF5I GGATG 3 cut(s) 170, 750, 1028
BstFNI CGCG 3 cut(s) 299, 307, 627
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 2 cut(s) 182, 307
BstKTI GATC 3 cut(s) 316, 952, 1045
BstMAI GTCTC 2 cut(s) 205, 771
BstMBI GATC 3 cut(s) 313, 949, 1042
BstMWI GCNNNNNNNGC 3 cut(s) 141, 490, 900
BstNI CCWGG 4 cut(s) 149, 603, 745, 913
BstSCI CCNGG 8 cut(s) 147, 423, 501, 601, 743, 911, 964, 965
BstSFI CTRYAG 1 cut(s) 68
BstUI CGCG 3 cut(s) 299, 307, 627
BstV1I GCAGC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 1042
BstXI CCANNNNNNTGG 2 cut(s) 926, 1002
BstYI RGATCY 1 cut(s) 1042
BsuRI GGCC 2 cut(s) 493, 918
BtgI CCRYGG 2 cut(s) 297, 546
BtsCI GGATG 3 cut(s) 170, 750, 1028
BtsI GCAGTG 1 cut(s) 889
BtsIMutI CAGTG 4 cut(s) 80, 765, 889, 1012
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 137
CfoI GCGC 2 cut(s) 182, 307
Cfr10I RCCGGY 2 cut(s) 588, 704
Cfr13I GGNCC 6 cut(s) 364, 586, 612, 690, 916, 989
Cfr42I CCGCGG 1 cut(s) 300
Cfr9I CCCGGG 1 cut(s) 965
CpoI CGGWCCG 1 cut(s) 690
CsiI ACCWGGT 1 cut(s) 147
Csp6I GTAC 5 cut(s) 73, 349, 406, 472, 686
CspAI ACCGGT 2 cut(s) 588, 704
CspI CGGWCCG 1 cut(s) 690
CviAII CATG 5 cut(s) 112, 216, 616, 785, 883
CviQI GTAC 5 cut(s) 73, 349, 406, 472, 686
DdeI CTNAG 2 cut(s) 341, 671
DinI GGCGCC 1 cut(s) 181
DpnI GATC 3 cut(s) 315, 951, 1044
DpnII GATC 3 cut(s) 313, 949, 1042
DraIII CACNNNGTG 1 cut(s) 357
EaeI YGGCCR 1 cut(s) 491
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 441
Eco47I GGWCC 5 cut(s) 364, 586, 612, 690, 989
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 965
EcoICRI GAGCTC 1 cut(s) 439
EcoO109I RGGNCCY 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 453
EcoRII CCWGG 4 cut(s) 147, 601, 743, 911
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 441
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
FaeI CATG 5 cut(s) 115, 219, 619, 788, 886
FalI AAGNNNNNCTT 2 cut(s) 844, 876
FaqI GGGAC 5 cut(s) 518, 599, 625, 761, 806
FatI CATG 5 cut(s) 111, 215, 615, 784, 882
FauI CCCGC 3 cut(s) 304, 602, 704
Fnu4HI GCNGC 8 cut(s) 123, 164, 178, 300, 303, 323, 482, 530
FokI GGATG 3 cut(s) 157, 737, 1035
FriOI GRGCYC 1 cut(s) 441
Fsp4HI GCNGC 8 cut(s) 123, 164, 178, 300, 303, 323, 482, 530
FspBI CTAG 2 cut(s) 372, 870
GlaI GCGC 2 cut(s) 181, 306
GluI GCNGC 8 cut(s) 123, 164, 178, 300, 303, 323, 482, 530
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 493, 918
HapII CCGG 9 cut(s) 174, 425, 458, 494, 502, 589, 705, 966, 987
HhaI GCGC 2 cut(s) 182, 307
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 5 cut(s) 115, 219, 619, 788, 886
Hin6I GCGC 2 cut(s) 180, 305
HinP1I GCGC 2 cut(s) 180, 305
HincII GTYRAC 1 cut(s) 543
HindII GTYRAC 1 cut(s) 543
HinfI GANTC 5 cut(s) 506, 597, 819, 979, 1009
HpaII CCGG 9 cut(s) 174, 425, 458, 494, 502, 589, 705, 966, 987
HphI GGTGA 3 cut(s) 181, 220, 308
Hpy166II GTNNAC 6 cut(s) 65, 351, 406, 474, 543, 992
Hpy188I TCNGA 3 cut(s) 155, 656, 674
Hpy188III TCNNGA 3 cut(s) 251, 809, 870
Hpy8I GTNNAC 6 cut(s) 65, 351, 406, 474, 543, 992
Hpy99I CGWCG 3 cut(s) 212, 584, 650
HpyAV CCTTC 1 cut(s) 424
HpyCH4III ACNGT 5 cut(s) 72, 357, 478, 547, 760
HpyCH4IV ACGT 2 cut(s) 116, 525
HpyCH4V TGCA 6 cut(s) 4, 135, 330, 719, 770, 827
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 490, 900
HpyF3I CTNAG 2 cut(s) 341, 671
HpySE526I ACGT 2 cut(s) 116, 525
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 5 cut(s) 115, 219, 619, 788, 886
HspAI GCGC 2 cut(s) 180, 305
KasI GGCGCC 1 cut(s) 179
KspI CCGCGG 1 cut(s) 300
Kzo9I GATC 3 cut(s) 313, 949, 1042
LmnI GCTCC 3 cut(s) 436, 444, 728
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 1 cut(s) 625
MabI ACCWGGT 1 cut(s) 147
MaeI CTAG 2 cut(s) 372, 870
MaeII ACGT 2 cut(s) 116, 525
MalI GATC 3 cut(s) 315, 951, 1044
MboI GATC 3 cut(s) 313, 949, 1042
MboII GAAGA 3 cut(s) 406, 565, 568
MflI RGATCY 1 cut(s) 1042
MhlI GDGCHC 1 cut(s) 441
MluCI AATT 5 cut(s) 156, 453, 565, 800, 939
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 2 cut(s) 500, 828
MmeI TCCRAC 1 cut(s) 133
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 666, 938
MspA1I CMGCKG 3 cut(s) 275, 299, 1037
MspI CCGG 9 cut(s) 174, 425, 458, 494, 502, 589, 705, 966, 987
MspR9I CCNGG 8 cut(s) 149, 425, 503, 603, 745, 913, 966, 967
Mva1269I GAATGC 1 cut(s) 837
MvaI CCWGG 4 cut(s) 149, 603, 745, 913
MvnI CGCG 3 cut(s) 299, 307, 627
MwoI GCNNNNNNNGC 3 cut(s) 141, 490, 900
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 4 cut(s) 425, 503, 966, 967
NdeII GATC 3 cut(s) 313, 949, 1042
NlaIII CATG 5 cut(s) 115, 219, 619, 788, 886
NlaIV GGNNCC 6 cut(s) 181, 462, 480, 587, 613, 917
NsiI ATGCAT 1 cut(s) 6
PaqCI CACCTGC 1 cut(s) 327
PasI CCCWGGG 1 cut(s) 912
PctI GAATGC 1 cut(s) 837
PfeI GAWTC 3 cut(s) 597, 979, 1009
PinAI ACCGGT 2 cut(s) 588, 704
PkrI GCNGC 8 cut(s) 124, 165, 179, 301, 304, 324, 483, 531
PleI GAGTC 2 cut(s) 500, 827
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 2 cut(s) 500, 827
PpuMI RGGWCCY 1 cut(s) 364
PshBI ATTAAT 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 364
Psp6I CCWGG 4 cut(s) 147, 601, 743, 911
PspGI CCWGG 4 cut(s) 147, 601, 743, 911
PspN4I GGNNCC 6 cut(s) 181, 462, 480, 587, 613, 917
PspPI GGNCC 6 cut(s) 364, 586, 612, 690, 916, 989
PspPPI RGGWCCY 1 cut(s) 364
PsuI RGATCY 1 cut(s) 1042
PvuII CAGCTG 1 cut(s) 1037
RsaI GTAC 5 cut(s) 74, 350, 407, 473, 687
RsaNI GTAC 5 cut(s) 73, 349, 406, 472, 686
Rsr2I CGGWCCG 1 cut(s) 690
RsrII CGGWCCG 1 cut(s) 690
SacI GAGCTC 1 cut(s) 441
SacII CCGCGG 1 cut(s) 300
SaqAI TTAA 2 cut(s) 666, 938
SatI GCNGC 8 cut(s) 123, 164, 178, 300, 303, 323, 482, 530
Sau3AI GATC 3 cut(s) 313, 949, 1042
Sau96I GGNCC 6 cut(s) 364, 586, 612, 690, 916, 989
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 2 cut(s) 500, 828
ScrFI CCNGG 8 cut(s) 149, 425, 503, 603, 745, 913, 966, 967
SduI GDGCHC 1 cut(s) 441
SexAI ACCWGGT 1 cut(s) 147
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 1 cut(s) 68
SfoI GGCGCC 1 cut(s) 181
Sfr303I CCGCGG 1 cut(s) 300
SgrBI CCGCGG 1 cut(s) 300
SinI GGWCC 5 cut(s) 364, 586, 612, 690, 989
SmaI CCCGGG 1 cut(s) 967
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 5 cut(s) 156, 453, 565, 800, 939
SspDI GGCGCC 1 cut(s) 179
SspMI CTAG 2 cut(s) 372, 870
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 8 cut(s) 147, 423, 501, 601, 743, 911, 964, 965
TaaI ACNGT 5 cut(s) 72, 357, 478, 547, 760
TaiI ACGT 2 cut(s) 119, 528
TaqI TCGA 4 cut(s) 252, 387, 645, 952
TasI AATT 5 cut(s) 156, 453, 565, 800, 939
TatI WGTACW 3 cut(s) 72, 348, 405
TauI GCSGC 7 cut(s) 125, 180, 302, 305, 325, 484, 532
TfiI GAWTC 3 cut(s) 597, 979, 1009
Tru1I TTAA 2 cut(s) 666, 938
Tru9I TTAA 2 cut(s) 666, 938
TscAI CASTG 4 cut(s) 87, 765, 896, 1019
TseI GCWGC 1 cut(s) 163
TspDTI ATGAA 2 cut(s) 563, 801
TspGWI ACGGA 1 cut(s) 703
TspMI CCCGGG 1 cut(s) 965
TspRI CASTG 4 cut(s) 87, 765, 896, 1019
VpaK11BI GGWCC 5 cut(s) 364, 586, 612, 690, 989
VspI ATTAAT 1 cut(s) 666
XapI RAATTY 2 cut(s) 156, 453
XbaI TCTAGA 1 cut(s) 869
XcmI CCANNNNNNNNNTGG 1 cut(s) 999
XmaI CCCGGG 1 cut(s) 965
XspI CTAG 2 cut(s) 372, 870
ZrmI AGTACT 1 cut(s) 74
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.