Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009758

Physical Location & Seq

Genomic Coordinates Reverse (-)

15382973 .. 15387768

Gene Structure 4796 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1050600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1899 bp

ATGGCTTTCCAAACTCCACTACAGTACTTTTCACTGAAACCCAAAAGAGCCAACCTTTATTACATGACGGTAGCCGCAGTCCTCTGCACGATCTGCTACCTTGTCGGAATTTGGCAGCACTCAACCAGCCGCGCCGCAATTTACACACCTGTCTCCGCCGTCACCTCATGTCCTCCAATCACCACAAACACAACAATCACTCTCGACTTCAACGCCCACCACCGCGCCGAGGACCTCCCCCTACCTCCCGTGGCTGCGCGTGTGGCTCACCTGCCGGCCTGCGACGCCAAGCACAGCGAGTACACCCCGTGCGAGGACGTCACGAGATCGCTCAAGTTCGACAGGGATAGGTTGGTGTACAGGGAGAGGCATTGCCCGGACAAGGAGGAGCTTCTGAAGTGTCGGATTCCAGCGCCTCACGGCTACACGGTACCGTTTCGGTGGCCGGAGAGCCGAGAATCGGTTTGGTATGCAAATGTGCCGCACAAGGAGTTGACCGTGGAGAAGAAGATGCAGAATTGGGTTCATTACGAAGGGGATCGGTTTAGATTCCCTGGTGGAGGGACCATGTTTCCTCGCGGTGCAGATGCGTATATTGATGATATCGGAAAGTTGATTAATCTCAGAGATGGGTCTATACGGACCGCCATCGATACCGGTTGCGGGGTTGCAAGTTGGGGAGCTTACCTTTTGTCCAGGGACATTCTAACAGTGTCATTTGCACCAAGAGACACACATGAAGCACAAGTTCAATTTGCTCTTGAAAGAGGAGTTCCTGCATTGATTGGCATTCTTGCTTCCAAAAGACTTCCTTACCCCTCAAGAGCTTTTGACATGGCTCACTGCTCTCGATGCCTCATCCCCTGGGGCCAATATGATGGGCTTTACTTGATTGAAGTTGATCGAGTTTTACGCCCCGGTGGGTACTGGATTCTTTCCGGGCCACCAATAAACTGGGAGAATCACTGGAAAGGATGGGAAAGAACAGCTGAAGATCTTAAAGCTGAGCAGACAACGATTGAGAATGTAGCAAAAAGATTGTGTTGGAAGAAACTGAAGCAGAAGGGTGACCTTGCAATTTGGCAAAAACCCACTAACCATGTTCACTGTAAGGTCAACAGAAAGCTCTTCAAGAAACCATCCTTCTGCCAAGCTCAAGATCCTGACACTGCATGGTATACGAAAATGGAGGATTGTTTGACCCCACTTCCTGGAGTGAACAACATAAAGGAAATTGCAGGAGGGCAATTGGCTAAATGGCCAGAGAGGCTAAACACAGTCCCTCCAAGAATCAGCAGTGGGAGTTTGACAGGAATCACAGCTGAGACCTTCAGAGAAAACACAGAGCTTTGGAAGAAAAGAGTAGAATATTACAAGACCGTGGATTATCAGTTGGCAGAGGCCGGGAGGTACCGGAATCTGCTCGATATGAACGCTTACTTGGGCGGCTTTGCAGCTGTACTTGTTCATGACCCTGTGTGGGTTATGAACATTGTCCCTGTTGAGGCTGAGGTTAACGCCCTTGGAGCCATTTATGAACGAGGATTGATTGGAACTTATCAGAATTGGTGTGAAGCTATGTCTACTTACCCAAGAACCTATGACTTGATTCACTCTGATTCAGTTTTTACCCTCTACAAGGACAGATGTGAAGCGGAAGATATTCTTTTAGAAATGGATAGGATTTTAAGGCCAGAGGGCAGTATAATCTTCCGTGATGACGTGGACGTGTTGGTGAAAGTCAAGAGCATCTTGGATGCAATGCAATACGACGCCAGAATCGTCGACCATGAAAACGGACCAAAGGTGAGAGAGAAGATTTTGTTGGCGGTCAAGCAGTACTGGACTGCCCCAGCTCCTGCCCTAGAAACTCAAGAACAAAGCAAATCGAATTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

633

Amino Acids

72.06

Weight (kDa)

7.81

Isoelectric Point (pI)

36.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	100 - 612	5e-226	Putative S-adenosyl-L-methionine-dependent methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009758)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45750 AT4G00750
fragaria_vesca	FvH4_7g13290
malus_domestica	MD01G1050600.v1.1 MD07G1127900.v1.1
prunus_persica	Prupe.2G156500_v2.0.a1
pyrus_communis	pycom01g07540 pycom01g07550 pycom07g12340
rosa_chinensis	RchiOBHm_Chr1g0352551
rosa_laevigata	RLG00000028331
rosa_multiflora	Rmu_co8293159.1_g000001 Rmu_sc0000911.1_g000032
rosa_roxburghii	Rroxscaffold_4G00302640
rosa_rugosa	Rorug01G0230000
rosa_samantha	Rh1AG243500 Rh1BG214100 Rh1CG227000 Rh1DG240100
rosa_wichuraiana	Rw1G021280 Rw1G021310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	279
AatII	GACGTC	1 cut(s)	321
Acc36I	ACCTGC	1 cut(s)	279
Acc65I	GGTACC	2 cut(s)	430, 1410
AccB1I	GGYRCC	2 cut(s)	430, 1410
AccB7I	CCANNNNNTGG	1 cut(s)	1211
AccI	GTMKAC	3 cut(s)	1178, 1583, 1785
AccII	CGCG	4 cut(s)	132, 225, 259, 579
AclWI	GGATC	2 cut(s)	546, 1154
AcoI	YGGCCR	2 cut(s)	443, 1259
AcsI	RAATTY	2 cut(s)	108, 1891
AcuI	CTGAAG	4 cut(s)	416, 1011, 1076, 1315
AcyI	GRCGYC	3 cut(s)	285, 318, 1773
AdeI	CACNNNGTG	1 cut(s)	309
AfaI	GTAC	8 cut(s)	26, 302, 359, 432, 926, 1412, 1461, 1841
AflIII	ACRYGT	1 cut(s)	1728
AgeI	ACCGGT	1 cut(s)	656
AgsI	TTSAA	5 cut(s)	211, 752, 764, 896, 1132
AjiI	CACGTC	2 cut(s)	1723, 1729
AjnI	CCWGG	4 cut(s)	553, 695, 863, 1210
AjuI	GAANNNNNNNTTGG	4 cut(s)	1424, 1456, 1808, 1840
Alw26I	GTCTC	3 cut(s)	157, 723, 1319
AlwI	GGATC	2 cut(s)	546, 1154
AlwNI	CAGNNNCTG	1 cut(s)	1859
AoxI	GGCC	7 cut(s)	276, 443, 868, 941, 1259, 1401, 1691
ApeKI	GCWGC	3 cut(s)	115, 254, 1454
ApoI	RAATTY	2 cut(s)	108, 1891
AseI	ATTAAT	1 cut(s)	618
AsiGI	ACCGGT	1 cut(s)	656
Asp700I	GAANNNNTTC	1 cut(s)	1662
Asp718I	GGTACC	2 cut(s)	430, 1410
AspLEI	GCGC	4 cut(s)	134, 227, 259, 415
AspS9I	GGNCC	6 cut(s)	232, 564, 642, 868, 941, 1799
AsuC2I	CCSGG	4 cut(s)	377, 918, 940, 1405
AsuHPI	GGTGA	6 cut(s)	154, 172, 260, 1079, 1747, 1819
AvaII	GGWCC	4 cut(s)	232, 564, 642, 1799
BalI	TGGCCA	1 cut(s)	1261
BanI	GGYRCC	2 cut(s)	430, 1410
BauI	CACGAG	1 cut(s)	322
BbvCI	CCTCAGC	1 cut(s)	1509
BbvI	GCAGC	3 cut(s)	127, 241, 1466
BccI	CCATC	5 cut(s)	623, 656, 872, 969, 1147
BceAI	ACGGC	2 cut(s)	143, 436
BciT130I	CCWGG	4 cut(s)	555, 697, 865, 1212
BcnI	CCSGG	4 cut(s)	377, 918, 940, 1405
BcoDI	GTCTC	3 cut(s)	157, 723, 1319
BfaI	CTAG	1 cut(s)	1865
BfmI	CTRYAG	1 cut(s)	20
BfoI	RGCGCY	1 cut(s)	416
BfuAI	ACCTGC	1 cut(s)	279
BglI	GCCNNNNNGGC	1 cut(s)	1267
BglII	AGATCT	1 cut(s)	994
BisI	GCNGC	8 cut(s)	75, 116, 130, 135, 255, 482, 1447, 1455
BlpI	GCTNAGC	1 cut(s)	1005
BlsI	GCNGC	8 cut(s)	76, 117, 131, 136, 256, 483, 1448, 1456
BmcAI	AGTACT	2 cut(s)	26, 1841
Bme1390I	CCNGG	8 cut(s)	377, 555, 697, 865, 918, 940, 1212, 1405
Bme18I	GGWCC	4 cut(s)	232, 564, 642, 1799
BmgBI	CACGTC	2 cut(s)	1723, 1729
BmgT120I	GGNCC	6 cut(s)	232, 564, 642, 868, 941, 1799
BmiI	GGNNCC	5 cut(s)	432, 565, 869, 1412, 1528
BmrFI	CCNGG	8 cut(s)	377, 555, 697, 865, 918, 940, 1212, 1405
BmrI	ACTGGG	1 cut(s)	964
BmsI	GCATC	5 cut(s)	501, 577, 842, 1747, 1758
BmuI	ACTGGG	1 cut(s)	964
BpmI	CTGGAG	1 cut(s)	1233
Bpu10I	CCTNAGC	1 cut(s)	1509
Bpu1102I	GCTNAGC	1 cut(s)	1005
BpuEI	CTTGAG	4 cut(s)	317, 805, 1140, 1857
BpuMI	CCSGG	4 cut(s)	377, 918, 940, 1405
Bsa29I	ATCGAT	1 cut(s)	651
BsaHI	GRCGYC	3 cut(s)	285, 318, 1773
BsaI	GGTCTC	1 cut(s)	1319
BsaWI	WCCGGW	2 cut(s)	656, 1413
BsaXI	ACNNNNNCTCC	2 cut(s)	1267, 1297
Bse118I	RCCGGY	2 cut(s)	274, 656
Bse1I	ACTGG	4 cut(s)	932, 959, 971, 1847
Bse3DI	GCAATG	2 cut(s)	370, 1767
BseBI	CCWGG	4 cut(s)	555, 697, 865, 1212
BseCI	ATCGAT	1 cut(s)	651
BseGI	GGATG	4 cut(s)	858, 980, 1139, 1762
BseMI	GCAATG	2 cut(s)	370, 1767
BseMII	CTCAG	4 cut(s)	637, 996, 1314, 1500
BseNI	ACTGG	4 cut(s)	932, 959, 971, 1847
BseRI	GAGGAG	2 cut(s)	401, 783
BseXI	GCAGC	3 cut(s)	127, 241, 1466
BseYI	CCCAGC	1 cut(s)	1852
BsgI	GTGCAG	2 cut(s)	70, 603
Bsh1236I	CGCG	4 cut(s)	132, 225, 259, 579
BshFI	GGCC	7 cut(s)	278, 445, 870, 943, 1261, 1403, 1693
BshNI	GGYRCC	2 cut(s)	430, 1410
BshTI	ACCGGT	1 cut(s)	656
BshVI	ATCGAT	1 cut(s)	651
BsiSI	CCGG	8 cut(s)	275, 377, 446, 657, 918, 939, 1404, 1414
BslFI	GGGAC	4 cut(s)	577, 713, 1265, 1481
BsmAI	GTCTC	3 cut(s)	157, 723, 1319
BsmFI	GGGAC	4 cut(s)	577, 713, 1265, 1481
BsmI	GAATGC	1 cut(s)	789
BsnI	GGCC	7 cut(s)	278, 445, 870, 943, 1261, 1403, 1693
Bso31I	GGTCTC	1 cut(s)	1319
Bsp1407I	TGTACA	1 cut(s)	357
Bsp143I	GATC	6 cut(s)	90, 326, 538, 901, 994, 1159
Bsp1720I	GCTNAGC	1 cut(s)	1005
BspANI	GGCC	7 cut(s)	278, 445, 870, 943, 1261, 1403, 1693
BspCNI	CTCAG	4 cut(s)	636, 997, 1315, 1501
BspDI	ATCGAT	1 cut(s)	651
BspFNI	CGCG	4 cut(s)	132, 225, 259, 579
BspHI	TCATGA	1 cut(s)	1468
BspLI	GGNNCC	5 cut(s)	432, 565, 869, 1412, 1528
BspMI	ACCTGC	1 cut(s)	279
BspPI	GGATC	2 cut(s)	546, 1154
BspQI	GCTCTTC	1 cut(s)	1133
BspT107I	GGYRCC	2 cut(s)	430, 1410
BspTNI	GGTCTC	1 cut(s)	1319
BsrDI	GCAATG	2 cut(s)	370, 1767
BsrFI	RCCGGY	2 cut(s)	274, 656
BsrGI	TGTACA	1 cut(s)	357
BsrI	ACTGG	4 cut(s)	932, 959, 971, 1847
BssAI	RCCGGY	2 cut(s)	274, 656
BssMI	GATC	6 cut(s)	90, 326, 538, 901, 994, 1159
BssNAI	GTATAC	1 cut(s)	1179
BssNI	GRCGYC	3 cut(s)	285, 318, 1773
BssSI	CACGAG	1 cut(s)	322
BssT1I	CCWWGG	1 cut(s)	1522
Bst1107I	GTATAC	1 cut(s)	1179
Bst2BI	CACGAG	1 cut(s)	322
Bst2UI	CCWGG	4 cut(s)	555, 697, 865, 1212
Bst4CI	ACNGT	9 cut(s)	24, 70, 430, 435, 499, 712, 1109, 1279, 1381
Bst6I	CTCTTC	1 cut(s)	1133
BstACI	GRCGYC	3 cut(s)	285, 318, 1773
BstAPI	GCANNNNNTGC	1 cut(s)	93
BstAUI	TGTACA	1 cut(s)	357
BstC8I	GCNNGC	2 cut(s)	276, 280
BstDEI	CTNAG	4 cut(s)	623, 1005, 1323, 1509
BstDSI	CCRYGG	3 cut(s)	249, 498, 1380
BstEII	GGTNACC	1 cut(s)	1067
BstENI	CCTNNNNNAGG	2 cut(s)	558, 1637
BstF5I	GGATG	4 cut(s)	858, 980, 1139, 1762
BstFNI	CGCG	4 cut(s)	132, 225, 259, 579
BstH2I	RGCGCY	1 cut(s)	416
BstHHI	GCGC	4 cut(s)	134, 227, 259, 415
BstKTI	GATC	6 cut(s)	93, 329, 541, 904, 997, 1162
BstMAI	GTCTC	3 cut(s)	157, 723, 1319
BstMBI	GATC	6 cut(s)	90, 326, 538, 901, 994, 1159
BstMWI	GCNNNNNNNGC	6 cut(s)	93, 263, 284, 852, 1267, 1526
BstNI	CCWGG	4 cut(s)	555, 697, 865, 1212
BstPI	GGTNACC	1 cut(s)	1067
BstSCI	CCNGG	8 cut(s)	375, 553, 695, 863, 916, 938, 1210, 1403
BstSFI	CTRYAG	1 cut(s)	20
BstUI	CGCG	4 cut(s)	132, 225, 259, 579
BstV1I	GCAGC	3 cut(s)	127, 241, 1466
BstX2I	RGATCY	2 cut(s)	994, 1159
BstXI	CCANNNNNNTGG	2 cut(s)	878, 954
BstYI	RGATCY	2 cut(s)	994, 1159
BstZ17I	GTATAC	1 cut(s)	1179
Bsu15I	ATCGAT	1 cut(s)	651
BsuRI	GGCC	7 cut(s)	278, 445, 870, 943, 1261, 1403, 1693
BsuTUI	ATCGAT	1 cut(s)	651
BtgI	CCRYGG	3 cut(s)	249, 498, 1380
BtrI	CACGTC	2 cut(s)	1723, 1729
BtsCI	GGATG	4 cut(s)	858, 980, 1139, 1762
BtsI	GCAGTG	3 cut(s)	841, 1167, 1303
BtsIMutI	CAGTG	7 cut(s)	32, 717, 841, 964, 1105, 1167, 1303
BveI	ACCTGC	1 cut(s)	279
Cac8I	GCNNGC	2 cut(s)	276, 280
CaiI	CAGNNNCTG	1 cut(s)	1859
CciI	TCATGA	1 cut(s)	1468
CfoI	GCGC	4 cut(s)	134, 227, 259, 415
Cfr10I	RCCGGY	2 cut(s)	274, 656
Cfr13I	GGNCC	6 cut(s)	232, 564, 642, 868, 941, 1799
ClaI	ATCGAT	1 cut(s)	651
CpoI	CGGWCCG	1 cut(s)	642
CseI	GACGC	2 cut(s)	293, 1781
Csp6I	GTAC	8 cut(s)	25, 301, 358, 431, 925, 1411, 1460, 1840
CspAI	ACCGGT	1 cut(s)	656
CspI	CGGWCCG	1 cut(s)	642
CviAII	CATG	9 cut(s)	64, 168, 568, 737, 835, 1100, 1173, 1469, 1790
CviQI	GTAC	8 cut(s)	25, 301, 358, 431, 925, 1411, 1460, 1840
DdeI	CTNAG	4 cut(s)	623, 1005, 1323, 1509
DpnI	GATC	6 cut(s)	92, 328, 540, 903, 996, 1161
DpnII	GATC	6 cut(s)	90, 326, 538, 901, 994, 1159
DraIII	CACNNNGTG	1 cut(s)	309
EaeI	YGGCCR	2 cut(s)	443, 1259
Eam1104I	CTCTTC	1 cut(s)	1133
EarI	CTCTTC	1 cut(s)	1133
EciI	GGCGGA	1 cut(s)	145
Eco130I	CCWWGG	1 cut(s)	1522
Eco31I	GGTCTC	1 cut(s)	1319
Eco32I	GATATC	1 cut(s)	604
Eco47I	GGWCC	4 cut(s)	232, 564, 642, 1799
Eco57I	CTGAAG	4 cut(s)	416, 1011, 1076, 1315
Eco91I	GGTNACC	1 cut(s)	1067
EcoNI	CCTNNNNNAGG	2 cut(s)	558, 1637
EcoO109I	RGGNCCY	1 cut(s)	232
EcoO65I	GGTNACC	1 cut(s)	1067
EcoRI	GAATTC	1 cut(s)	1891
EcoRII	CCWGG	4 cut(s)	553, 695, 863, 1210
EcoRV	GATATC	1 cut(s)	604
EcoT14I	CCWWGG	1 cut(s)	1522
ErhI	CCWWGG	1 cut(s)	1522
FaeI	CATG	9 cut(s)	67, 171, 571, 740, 838, 1103, 1176, 1472, 1793
FalI	AAGNNNNNCTT	6 cut(s)	796, 828, 1650, 1682, 1736, 1768
FaqI	GGGAC	4 cut(s)	577, 713, 1265, 1481
FatI	CATG	9 cut(s)	63, 167, 567, 736, 834, 1099, 1172, 1468, 1789
FauI	CCCGC	1 cut(s)	656
FblI	GTMKAC	3 cut(s)	1178, 1583, 1785
Fnu4HI	GCNGC	8 cut(s)	75, 116, 130, 135, 255, 482, 1447, 1455
FokI	GGATG	4 cut(s)	845, 987, 1126, 1769
Fsp4HI	GCNGC	8 cut(s)	75, 116, 130, 135, 255, 482, 1447, 1455
FspBI	CTAG	1 cut(s)	1865
GlaI	GCGC	4 cut(s)	133, 226, 258, 414
GluI	GCNGC	8 cut(s)	75, 116, 130, 135, 255, 482, 1447, 1455
GsaI	CCCAGC	1 cut(s)	1856
GsuI	CTGGAG	1 cut(s)	1233
HaeII	RGCGCY	1 cut(s)	416
HaeIII	GGCC	7 cut(s)	278, 445, 870, 943, 1261, 1403, 1693
HapII	CCGG	8 cut(s)	275, 377, 446, 657, 918, 939, 1404, 1414
HgaI	GACGC	2 cut(s)	293, 1781
HhaI	GCGC	4 cut(s)	134, 227, 259, 415
Hin1I	GRCGYC	3 cut(s)	285, 318, 1773
Hin1II	CATG	9 cut(s)	67, 171, 571, 740, 838, 1103, 1176, 1472, 1793
Hin6I	GCGC	4 cut(s)	132, 225, 257, 413
HinP1I	GCGC	4 cut(s)	132, 225, 257, 413
HincII	GTYRAC	4 cut(s)	495, 1117, 1516, 1786
HindII	GTYRAC	4 cut(s)	495, 1117, 1516, 1786
HpaI	GTTAAC	1 cut(s)	1516
HpaII	CCGG	8 cut(s)	275, 377, 446, 657, 918, 939, 1404, 1414
HphI	GGTGA	6 cut(s)	154, 172, 260, 1079, 1747, 1819
Hpy188I	TCNGA	8 cut(s)	107, 396, 405, 608, 626, 1334, 1563, 1618
Hpy99I	CGWCG	3 cut(s)	287, 1775, 1787
HpyAV	CCTTC	4 cut(s)	527, 1057, 1153, 1339
HpyCH4III	ACNGT	9 cut(s)	24, 70, 430, 435, 499, 712, 1109, 1279, 1381
HpyCH4IV	ACGT	3 cut(s)	318, 1722, 1728
HpyF10VI	GCNNNNNNNGC	6 cut(s)	93, 263, 284, 852, 1267, 1526
HpyF3I	CTNAG	4 cut(s)	623, 1005, 1323, 1509
HpySE526I	ACGT	3 cut(s)	318, 1722, 1728
Hsp92I	GRCGYC	3 cut(s)	285, 318, 1773
Hsp92II	CATG	9 cut(s)	67, 171, 571, 740, 838, 1103, 1176, 1472, 1793
HspAI	GCGC	4 cut(s)	132, 225, 257, 413
KpnI	GGTACC	2 cut(s)	434, 1414
KroI	GCCGGC	1 cut(s)	274
KroNI	GCCGGC	1 cut(s)	276
KspAI	GTTAAC	1 cut(s)	1516
Kzo9I	GATC	6 cut(s)	90, 326, 538, 901, 994, 1159
LguI	GCTCTTC	1 cut(s)	1133
LmnI	GCTCC	4 cut(s)	388, 680, 1526, 1861
Lsp1109I	GCAGC	3 cut(s)	127, 241, 1466
LweI	GCATC	5 cut(s)	501, 577, 842, 1747, 1758
MaeI	CTAG	1 cut(s)	1865
MaeII	ACGT	3 cut(s)	318, 1722, 1728
MaeIII	GTNAC	3 cut(s)	160, 319, 1067
MalI	GATC	6 cut(s)	92, 328, 540, 903, 996, 1161
MboI	GATC	6 cut(s)	90, 326, 538, 901, 994, 1159
MboII	GAAGA	9 cut(s)	517, 520, 1004, 1060, 1120, 1366, 1670, 1702, 1828
MfeI	CAATTG	1 cut(s)	1247
MflI	RGATCY	2 cut(s)	994, 1159
MlsI	TGGCCA	1 cut(s)	1261
MluCI	AATT	9 cut(s)	108, 138, 517, 752, 1077, 1233, 1247, 1564, 1891
MluNI	TGGCCA	1 cut(s)	1261
MmeI	TCCRAC	3 cut(s)	85, 383, 1025
Mox20I	TGGCCA	1 cut(s)	1261
MroNI	GCCGGC	1 cut(s)	274
MroXI	GAANNNNTTC	1 cut(s)	1662
MscI	TGGCCA	1 cut(s)	1261
MseI	TTAA	4 cut(s)	618, 999, 1515, 1688
Msp20I	TGGCCA	1 cut(s)	1261
MspA1I	CMGCKG	3 cut(s)	989, 1322, 1457
MspI	CCGG	8 cut(s)	275, 377, 446, 657, 918, 939, 1404, 1414
MspR9I	CCNGG	8 cut(s)	377, 555, 697, 865, 918, 940, 1212, 1405
MunI	CAATTG	1 cut(s)	1247
Mva1269I	GAATGC	1 cut(s)	789
MvaI	CCWGG	4 cut(s)	555, 697, 865, 1212
MvnI	CGCG	4 cut(s)	132, 225, 259, 579
MwoI	GCNNNNNNNGC	6 cut(s)	93, 263, 284, 852, 1267, 1526
NaeI	GCCGGC	1 cut(s)	276
NciI	CCSGG	4 cut(s)	377, 918, 940, 1405
NdeII	GATC	6 cut(s)	90, 326, 538, 901, 994, 1159
NgoMIV	GCCGGC	1 cut(s)	274
NlaIII	CATG	9 cut(s)	67, 171, 571, 740, 838, 1103, 1176, 1472, 1793
NlaIV	GGNNCC	5 cut(s)	432, 565, 869, 1412, 1528
NmeAIII	GCCGAG	2 cut(s)	253, 479
NmuCI	GTSAC	3 cut(s)	160, 319, 1067
PagI	TCATGA	1 cut(s)	1468
PaqCI	CACCTGC	1 cut(s)	279
PasI	CCCWGGG	1 cut(s)	864
PciSI	GCTCTTC	1 cut(s)	1133
PcsI	WCGNNNNNNNCGW	1 cut(s)	1779
PctI	GAATGC	1 cut(s)	789
PdiI	GCCGGC	1 cut(s)	276
PdmI	GAANNNNTTC	1 cut(s)	1662
PflMI	CCANNNNNTGG	1 cut(s)	1211
PfoI	TCCNGGA	1 cut(s)	1210
PinAI	ACCGGT	1 cut(s)	656
PkrI	GCNGC	8 cut(s)	76, 117, 131, 136, 256, 483, 1448, 1456
PpuMI	RGGWCCY	1 cut(s)	232
PshBI	ATTAAT	1 cut(s)	618
Psp5II	RGGWCCY	1 cut(s)	232
Psp6I	CCWGG	4 cut(s)	553, 695, 863, 1210
PspEI	GGTNACC	1 cut(s)	1067
PspFI	CCCAGC	1 cut(s)	1852
PspGI	CCWGG	4 cut(s)	553, 695, 863, 1210
PspN4I	GGNNCC	5 cut(s)	432, 565, 869, 1412, 1528
PspPI	GGNCC	6 cut(s)	232, 564, 642, 868, 941, 1799
PspPPI	RGGWCCY	1 cut(s)	232
PstNI	CAGNNNCTG	1 cut(s)	1859
PsuI	RGATCY	2 cut(s)	994, 1159
PvuII	CAGCTG	3 cut(s)	989, 1322, 1457
RsaI	GTAC	8 cut(s)	26, 302, 359, 432, 926, 1412, 1461, 1841
RsaNI	GTAC	8 cut(s)	25, 301, 358, 431, 925, 1411, 1460, 1840
Rsr2I	CGGWCCG	1 cut(s)	642
RsrII	CGGWCCG	1 cut(s)	642
SalI	GTCGAC	1 cut(s)	1784
SapI	GCTCTTC	1 cut(s)	1133
SaqAI	TTAA	4 cut(s)	618, 999, 1515, 1688
SatI	GCNGC	8 cut(s)	75, 116, 130, 135, 255, 482, 1447, 1455
Sau3AI	GATC	6 cut(s)	90, 326, 538, 901, 994, 1159
Sau96I	GGNCC	6 cut(s)	232, 564, 642, 868, 941, 1799
ScaI	AGTACT	2 cut(s)	26, 1841
ScrFI	CCNGG	8 cut(s)	377, 555, 697, 865, 918, 940, 1212, 1405
SfaNI	GCATC	5 cut(s)	501, 577, 842, 1747, 1758
SfcI	CTRYAG	1 cut(s)	20
SinI	GGWCC	4 cut(s)	232, 564, 642, 1799
SmlI	CTYRAG	4 cut(s)	332, 820, 1155, 1872
SmoI	CTYRAG	4 cut(s)	332, 820, 1155, 1872
Sse9I	AATT	9 cut(s)	108, 138, 517, 752, 1077, 1233, 1247, 1564, 1891
SspI	AATATT	1 cut(s)	1370
SspMI	CTAG	1 cut(s)	1865
StyD4I	CCNGG	8 cut(s)	375, 553, 695, 863, 916, 938, 1210, 1403
StyI	CCWWGG	1 cut(s)	1522
TaaI	ACNGT	9 cut(s)	24, 70, 430, 435, 499, 712, 1109, 1279, 1381
TaiI	ACGT	3 cut(s)	321, 1725, 1731
TaqI	TCGA	8 cut(s)	204, 339, 651, 850, 904, 1425, 1785, 1889
TasI	AATT	9 cut(s)	108, 138, 517, 752, 1077, 1233, 1247, 1564, 1891
TatI	WGTACW	5 cut(s)	24, 300, 357, 1459, 1839
TauI	GCSGC	5 cut(s)	77, 132, 137, 484, 1449
Tru1I	TTAA	4 cut(s)	618, 999, 1515, 1688
Tru9I	TTAA	4 cut(s)	618, 999, 1515, 1688
TscAI	CASTG	7 cut(s)	39, 717, 848, 971, 1112, 1174, 1303
TseFI	GTSAC	3 cut(s)	160, 319, 1067
TseI	GCWGC	3 cut(s)	115, 254, 1454
Tsp45I	GTSAC	3 cut(s)	160, 319, 1067
TspDTI	ATGAA	8 cut(s)	515, 753, 1445, 1457, 1502, 1551, 1806, 1884
TspGWI	ACGGA	3 cut(s)	655, 1703, 1812
TspRI	CASTG	7 cut(s)	39, 717, 848, 971, 1112, 1174, 1303
Van91I	CCANNNNNTGG	1 cut(s)	1211
VpaK11BI	GGWCC	4 cut(s)	232, 564, 642, 1799
VspI	ATTAAT	1 cut(s)	618
XagI	CCTNNNNNAGG	2 cut(s)	558, 1637
XapI	RAATTY	2 cut(s)	108, 1891
XcmI	CCANNNNNNNNNTGG	1 cut(s)	951
XmiI	GTMKAC	3 cut(s)	1178, 1583, 1785
XmnI	GAANNNNTTC	1 cut(s)	1662
XspI	CTAG	1 cut(s)	1865
ZraI	GACGTC	1 cut(s)	319
ZrmI	AGTACT	2 cut(s)	26, 1841

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.