FvH4_7g13980

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009833
Physical Location & Seq
Reverse (-)
12448619 .. 12453542
4924 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g13980.t2

Sequence Viewer

Length: 1014 bp
ATGGATCGGAGGGACCATATGGCAATGTCGGGGTCAGCCTCTTATTACACGCCTAGATCAATAACTGGGTCTGGAACACAGTCTGGGTTACTTGGATCACCAGGCATTCATCCCTTATCTAATCCAAATGCAGTATTTCAGTCCAATATGGGGGCAGGTACTATTGGATCAACTTTACCAGTAGACCCCTCTTCAGCTATTTCACCTCATGGTGTCAATGTGGTTGCACCGTCTGTGGCGCCACAAGGTGAATCTTTGAAAAGGAAAAGAGGAAGACCTCGAAAATATGGACCTGATGGGACTGTTTCATTGGCATTGTCTCCATCGTCATCCTCCAATCCTGGGATGGTAGTTTCAAGCCCAAAGCGAGGTAGAGGGCGACCACCAGGGTCTGGGAAGAAGCAACAATTAGCGTCTCTTGGTGAAATGCTGTCTGGTTCAGCTGGGATGGGTTTCACTCCACATATTATCACCATTGCAATGGGAGAGGACATTGCAAAAAAAATAATGGCATTTTCACAGCAAAGCCCGAGAGCTCTCTGCGTCTTATCAGCCAATGGTGCTGTCTCTACCGTGACTCTTCGTCAGCCCTCCACTTCTGGTGGCACAGTCACATATGAGGGACGGTTTGAGATAATTTGTCTATCTGGCTCTTACTTGCTCACTGAAAGTGGTGGCTCCAGAAATCGAACTGGTGGTCTTAGTGTCTCCCTTGCTAGTCCTGATGGTCGTGTTGTTGGTGGTGGAGTAGGAGGAATGCTTATCGCAGCTGGTCCTGTACAGGTGATAGTAGGTAGCTTCATATGGGGTGATTCAAAGACAAAAAACAAGAAAAAAGAAGCTATAGAAGGTGGCACAGGCTTCGACCATCAGACAGTTGACAATTCAGTCGCATTTAATAGCATGCCACCCGATCAACAGTTTACCCAAGGGTCACCATTGGCAGTGTGGCCATCACCTCGACCATTGGATATGCGTAACAGTCATGGTGACATAGATTTGATGCGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

338

Amino Acids

34.72

Weight (kDa)

9.91

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 153 - 267 1.3e-25 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 887
Acc36I ACCTGC 1 cut(s) 146
AccB1I GGYRCC 1 cut(s) 238
AccB7I CCANNNNNTGG 1 cut(s) 392
AccI GTMKAC 1 cut(s) 183
AclWI GGATC 3 cut(s) 12, 103, 175
AcoI YGGCCR 1 cut(s) 950
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 239
AdeI CACNNNGTG 1 cut(s) 248
AfaI GTAC 2 cut(s) 160, 780
AfiI CCNNNNNNNGG 4 cut(s) 150, 342, 368, 392
AgsI TTSAA 3 cut(s) 259, 357, 816
AhdI GACNNNNNGTC 1 cut(s) 582
AjnI CCWGG 3 cut(s) 100, 340, 385
AluBI AGCT 6 cut(s) 197, 443, 536, 770, 798, 842
AluI AGCT 6 cut(s) 197, 443, 536, 770, 798, 842
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 4 cut(s) 324, 420, 571, 712
AlwI GGATC 3 cut(s) 12, 103, 175
AlwNI CAGNNNCTG 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 529
AoxI GGCC 1 cut(s) 950
ApeKI GCWGC 1 cut(s) 767
ArsI GACNNNNNNTTYG 2 cut(s) 682, 714
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 3 cut(s) 13, 290, 773
AvaI CYCGRG 1 cut(s) 529
AvaII GGWCC 3 cut(s) 13, 290, 773
BalI TGGCCA 1 cut(s) 952
BanI GGYRCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 538
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 538
BbvI GCAGC 1 cut(s) 779
BccI CCATC 7 cut(s) 290, 331, 340, 442, 719, 876, 961
BcgI CGANNNNNNTGC 2 cut(s) 844, 878
BciT130I CCWGG 3 cut(s) 102, 342, 387
BcoDI GTCTC 4 cut(s) 324, 420, 571, 712
BfaI CTAG 2 cut(s) 54, 717
BfmI CTRYAG 1 cut(s) 843
BfoI RGCGCY 1 cut(s) 242
BfuAI ACCTGC 1 cut(s) 146
BisI GCNGC 1 cut(s) 768
BlsI GCNGC 1 cut(s) 769
Bme1390I CCNGG 3 cut(s) 102, 342, 387
Bme18I GGWCC 3 cut(s) 13, 290, 773
BmeRI GACNNNNNGTC 1 cut(s) 582
BmeT110I CYCGRG 1 cut(s) 529
BmgT120I GGNCC 3 cut(s) 13, 290, 773
BmiI GGNNCC 3 cut(s) 14, 240, 679
BmrFI CCNGG 3 cut(s) 102, 342, 387
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 1 cut(s) 993
BmuI ACTGGG 1 cut(s) 75
BpiI GAAGAC 1 cut(s) 280
BpmI CTGGAG 1 cut(s) 664
BsaHI GRCGYC 1 cut(s) 239
BsaJI CCNNGG 3 cut(s) 341, 386, 928
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 342, 368, 392
Bse1I ACTGG 3 cut(s) 70, 179, 697
Bse3DI GCAATG 4 cut(s) 30, 474, 486, 492
BseBI CCWGG 3 cut(s) 102, 342, 387
BseDI CCNNGG 3 cut(s) 341, 386, 928
BseGI GGATG 4 cut(s) 109, 329, 351, 453
BseLI CCNNNNNNNGG 4 cut(s) 150, 342, 368, 392
BseMI GCAATG 4 cut(s) 30, 474, 486, 492
BseNI ACTGG 3 cut(s) 70, 179, 697
BseXI GCAGC 1 cut(s) 779
BseYI CCCAGC 1 cut(s) 443
BshFI GGCC 1 cut(s) 952
BshNI GGYRCC 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 538
BsiHKCI CYCGRG 1 cut(s) 529
BslFI GGGAC 3 cut(s) 26, 313, 636
BslI CCNNNNNNNGG 4 cut(s) 150, 342, 368, 392
BsmAI GTCTC 4 cut(s) 324, 420, 571, 712
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 3 cut(s) 26, 313, 636
BsmI GAATGC 2 cut(s) 105, 762
BsnI GGCC 1 cut(s) 952
BsoBI CYCGRG 1 cut(s) 529
Bsp1286I GDGCHC 1 cut(s) 538
Bsp1407I TGTACA 1 cut(s) 778
Bsp143I GATC 5 cut(s) 4, 56, 95, 167, 913
BspANI GGCC 1 cut(s) 952
BspLI GGNNCC 3 cut(s) 14, 240, 679
BspMI ACCTGC 1 cut(s) 146
BspPI GGATC 3 cut(s) 12, 103, 175
BspT107I GGYRCC 1 cut(s) 238
BsrDI GCAATG 4 cut(s) 30, 474, 486, 492
BsrGI TGTACA 1 cut(s) 778
BsrI ACTGG 3 cut(s) 70, 179, 697
BssECI CCNNGG 3 cut(s) 341, 386, 928
BssMI GATC 5 cut(s) 4, 56, 95, 167, 913
BssNI GRCGYC 1 cut(s) 239
BssT1I CCWWGG 1 cut(s) 928
Bst2UI CCWGG 3 cut(s) 102, 342, 387
Bst4CI ACNGT 9 cut(s) 81, 231, 304, 574, 610, 627, 877, 921, 983
Bst6I CTCTTC 2 cut(s) 196, 585
BstACI GRCGYC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 778
BstC8I GCNNGC 1 cut(s) 905
BstDEI CTNAG 1 cut(s) 701
BstEII GGTNACC 1 cut(s) 933
BstF5I GGATG 4 cut(s) 109, 329, 351, 453
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 5 cut(s) 7, 59, 98, 170, 916
BstMAI GTCTC 4 cut(s) 324, 420, 571, 712
BstMBI GATC 5 cut(s) 4, 56, 95, 167, 913
BstMWI GCNNNNNNNGC 1 cut(s) 560
BstNI CCWGG 3 cut(s) 102, 342, 387
BstNSI RCATGY 1 cut(s) 907
BstPI GGTNACC 1 cut(s) 933
BstSCI CCNGG 3 cut(s) 100, 340, 385
BstSFI CTRYAG 1 cut(s) 843
BstV1I GCAGC 1 cut(s) 779
BstV2I GAAGAC 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 481
BsuRI GGCC 1 cut(s) 952
BtsCI GGATG 4 cut(s) 109, 329, 351, 453
BtsI GCAGTG 1 cut(s) 951
BtsIMutI CAGTG 2 cut(s) 663, 951
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 905
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 3 cut(s) 13, 290, 773
CseI GACGC 2 cut(s) 402, 532
Csp6I GTAC 2 cut(s) 159, 779
CviAII CATG 3 cut(s) 209, 904, 986
CviQI GTAC 2 cut(s) 159, 779
DdeI CTNAG 1 cut(s) 701
DinI GGCGCC 1 cut(s) 240
DpnI GATC 5 cut(s) 6, 58, 97, 169, 915
DpnII GATC 5 cut(s) 4, 56, 95, 167, 913
DraIII CACNNNGTG 1 cut(s) 248
DrdI GACNNNNNNGTC 1 cut(s) 887
DriI GACNNNNNGTC 1 cut(s) 582
DseDI GACNNNNNNGTC 1 cut(s) 887
EaeI YGGCCR 1 cut(s) 950
Eam1104I CTCTTC 2 cut(s) 196, 585
Eam1105I GACNNNNNGTC 1 cut(s) 582
EarI CTCTTC 2 cut(s) 196, 585
Ecl136II GAGCTC 1 cut(s) 536
Eco130I CCWWGG 1 cut(s) 928
Eco24I GRGCYC 1 cut(s) 538
Eco47I GGWCC 3 cut(s) 13, 290, 773
Eco53kI GAGCTC 1 cut(s) 536
Eco57I CTGAAG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 529
Eco91I GGTNACC 1 cut(s) 933
EcoICRI GAGCTC 1 cut(s) 536
EcoO65I GGTNACC 1 cut(s) 933
EcoRII CCWGG 3 cut(s) 100, 340, 385
EcoT14I CCWWGG 1 cut(s) 928
EcoT38I GRGCYC 1 cut(s) 538
EgeI GGCGCC 1 cut(s) 240
EheI GGCGCC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 928
Esp3I CGTCTC 1 cut(s) 420
FaeI CATG 3 cut(s) 212, 907, 989
FaqI GGGAC 3 cut(s) 26, 313, 636
FatI CATG 3 cut(s) 208, 903, 985
FauNDI CATATG 3 cut(s) 18, 616, 803
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 1 cut(s) 768
FokI GGATG 4 cut(s) 96, 316, 358, 460
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 1 cut(s) 768
FspBI CTAG 2 cut(s) 54, 717
GlaI GCGC 1 cut(s) 240
GluI GCNGC 1 cut(s) 768
GsaI CCCAGC 1 cut(s) 447
GsuI CTGGAG 1 cut(s) 664
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 1 cut(s) 952
HgaI GACGC 2 cut(s) 402, 532
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 239
Hin1II CATG 3 cut(s) 212, 907, 989
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HinfI GANTC 3 cut(s) 251, 577, 812
Hpy166II GTNNAC 3 cut(s) 184, 880, 924
Hpy188I TCNGA 2 cut(s) 9, 873
Hpy188III TCNNGA 3 cut(s) 72, 681, 722
Hpy8I GTNNAC 3 cut(s) 184, 880, 924
HpyAV CCTTC 1 cut(s) 842
HpyCH4III ACNGT 9 cut(s) 81, 231, 304, 574, 610, 627, 877, 921, 983
HpyCH4V TGCA 4 cut(s) 131, 227, 479, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 560
HpyF3I CTNAG 1 cut(s) 701
Hsp92I GRCGYC 1 cut(s) 239
Hsp92II CATG 3 cut(s) 212, 907, 989
HspAI GCGC 1 cut(s) 239
KasI GGCGCC 1 cut(s) 238
Kzo9I GATC 5 cut(s) 4, 56, 95, 167, 913
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 1 cut(s) 779
LweI GCATC 1 cut(s) 993
MaeI CTAG 2 cut(s) 54, 717
MaeIII GTNAC 6 cut(s) 87, 574, 610, 933, 977, 989
MalI GATC 5 cut(s) 6, 58, 97, 169, 915
MboI GATC 5 cut(s) 4, 56, 95, 167, 913
MboII GAAGA 4 cut(s) 183, 285, 409, 572
MhlI GDGCHC 1 cut(s) 538
MlsI TGGCCA 1 cut(s) 952
MluCI AATT 3 cut(s) 407, 636, 883
MluNI TGGCCA 1 cut(s) 952
Mly113I GGCGCC 1 cut(s) 239
MlyI GAGTC 1 cut(s) 571
Mox20I TGGCCA 1 cut(s) 952
MscI TGGCCA 1 cut(s) 952
MseI TTAA 1 cut(s) 897
MslI CAYNNNNRTG 1 cut(s) 479
Msp20I TGGCCA 1 cut(s) 952
MspA1I CMGCKG 2 cut(s) 443, 770
MspR9I CCNGG 3 cut(s) 102, 342, 387
Mva1269I GAATGC 2 cut(s) 105, 762
MvaI CCWGG 3 cut(s) 102, 342, 387
MwoI GCNNNNNNNGC 1 cut(s) 560
NarI GGCGCC 1 cut(s) 239
NdeI CATATG 3 cut(s) 18, 616, 803
NdeII GATC 5 cut(s) 4, 56, 95, 167, 913
NlaIII CATG 3 cut(s) 212, 907, 989
NlaIV GGNNCC 3 cut(s) 14, 240, 679
NmuCI GTSAC 4 cut(s) 574, 610, 933, 989
NspI RCATGY 1 cut(s) 907
PaeI GCATGC 1 cut(s) 907
PctI GAATGC 2 cut(s) 105, 762
PfeI GAWTC 2 cut(s) 251, 812
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 1 cut(s) 769
PleI GAGTC 1 cut(s) 571
PluTI GGCGCC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 571
Psp124BI GAGCTC 1 cut(s) 538
Psp6I CCWGG 3 cut(s) 100, 340, 385
PspEI GGTNACC 1 cut(s) 933
PspFI CCCAGC 1 cut(s) 443
PspGI CCWGG 3 cut(s) 100, 340, 385
PspN4I GGNNCC 3 cut(s) 14, 240, 679
PspPI GGNCC 3 cut(s) 13, 290, 773
PstNI CAGNNNCTG 1 cut(s) 392
PvuII CAGCTG 2 cut(s) 443, 770
RsaI GTAC 2 cut(s) 160, 780
RsaNI GTAC 2 cut(s) 159, 779
RseI CAYNNNNRTG 1 cut(s) 479
SacI GAGCTC 1 cut(s) 538
SaqAI TTAA 1 cut(s) 897
SatI GCNGC 1 cut(s) 768
Sau3AI GATC 5 cut(s) 4, 56, 95, 167, 913
Sau96I GGNCC 3 cut(s) 13, 290, 773
SchI GAGTC 1 cut(s) 571
ScrFI CCNGG 3 cut(s) 102, 342, 387
SduI GDGCHC 1 cut(s) 538
SfaNI GCATC 1 cut(s) 993
SfcI CTRYAG 1 cut(s) 843
SfoI GGCGCC 1 cut(s) 240
SinI GGWCC 3 cut(s) 13, 290, 773
SmiMI CAYNNNNRTG 1 cut(s) 479
SphI GCATGC 1 cut(s) 907
Sse9I AATT 3 cut(s) 407, 636, 883
SspDI GGCGCC 1 cut(s) 238
SspMI CTAG 2 cut(s) 54, 717
SstI GAGCTC 1 cut(s) 538
StyD4I CCNGG 3 cut(s) 100, 340, 385
StyI CCWWGG 1 cut(s) 928
TaaI ACNGT 9 cut(s) 81, 231, 304, 574, 610, 627, 877, 921, 983
TaqI TCGA 4 cut(s) 280, 688, 864, 961
TasI AATT 3 cut(s) 407, 636, 883
TatI WGTACW 1 cut(s) 778
TfiI GAWTC 2 cut(s) 251, 812
Tru1I TTAA 1 cut(s) 897
Tru9I TTAA 1 cut(s) 897
TscAI CASTG 2 cut(s) 670, 951
TseFI GTSAC 4 cut(s) 574, 610, 933, 989
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 4 cut(s) 574, 610, 933, 989
TspDTI ATGAA 3 cut(s) 98, 297, 790
TspRI CASTG 2 cut(s) 670, 951
Van91I CCANNNNNTGG 1 cut(s) 392
VpaK11BI GGWCC 3 cut(s) 13, 290, 773
XceI RCATGY 1 cut(s) 907
XcmI CCANNNNNNNNNTGG 2 cut(s) 343, 945
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 2 cut(s) 54, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.