RLG00000028194

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009833
Physical Location & Seq
Forward (+)
19906383 .. 19909797
3415 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028194

Sequence Viewer

Length: 996 bp
ATGGCAATGTCGGGGTCAGCCTCTTATTACACACCGAGATCAATAACTGGGTCTGGAACACAGTCTGGGTTACTTGGATCACCAGGCATTCATCCCTTATCTAATCCAAATACAGTATTTCAGTCTAATATGGGGGCTGGTACGATTGGGTCAACATTACCAGTAGACCCTTCTTCGGCTATGTCACCTCATGGTGTCAATGTGGGTGCATCGTCTGTGGCGCCACAAGGTGAATCTGTGAAAAGGAAAAGAGGAAGACCTCGAAAATATGGACCTGATGGGACTGTTTCATTGGCATTGTCTCCGTCATCATCCTCCAATCCTGGGATGGTAGCTTCAAGCCCAAAGCGAGGTAGAGGGCGGCCACCAGGGTCTGGGAAGAAGCACCAATTAGCATCTCTTGGTGAAATGCTGTCTGGTTCAGCTGGAATGGGTTTCACTCCACATATTATCACCATCGCAATGGGAGAAGACATTGCTACAAAAATAATGGCATTTTCACAGCAAGGCCCGAGAGCTCTCTGTGTCTTATCAGCCAATGGTGCTGTCTCTACTGTGACTCTTCGTCAGCCCTCCACTTCTGGTGGCACAGTCACATATGAGGGACGGTTTGAGATAATTTGTCTATCAGGCTCTTACTTGCTCACTGAAAGTGGTGGCTCCAGAAATCGAACTGGTGGTCTTAGTGTCTCCCTTGCTAGTCCTGATGGTCGTGTTGTTGGTGGAGGAGTAGGAGGAATGCTTATTGCTGCTGGTCCTGTACAGGTGATAGTGGGTAGCTTCATATGGGGTGGTTCGAAGACAAAGAACAAGAAAAAAGAAGCTATAGAAGGTGTCACAGACTTCGACCAACAGACAGTTGACAATTCAGTTCCATTTAATAGCATGCCACCCGATCAAAATTTTACTCAAGGGTCATCGTTGGGCGTGTGGCCATCACCACGACCATTGGATATGCGTAACAGTCATGTTGACATTGATTTGATGCGAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

332

Amino Acids

33.88

Weight (kDa)

9.86

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 147 - 261 1.2e-25 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 220
AccB7I CCANNNNNTGG 1 cut(s) 374
AccI GTMKAC 1 cut(s) 165
AciI CCGC 1 cut(s) 361
AclWI GGATC 1 cut(s) 85
AcoI YGGCCR 2 cut(s) 362, 932
AcsI RAATTY 1 cut(s) 901
AcyI GRCGYC 1 cut(s) 221
AdeI CACNNNGTG 1 cut(s) 230
AfaI GTAC 2 cut(s) 142, 762
AfiI CCNNNNNNNGG 4 cut(s) 175, 324, 350, 374
AgsI TTSAA 1 cut(s) 339
AhdI GACNNNNNGTC 1 cut(s) 564
AjnI CCWGG 3 cut(s) 82, 322, 367
AluBI AGCT 5 cut(s) 335, 425, 518, 780, 824
AluI AGCT 5 cut(s) 335, 425, 518, 780, 824
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 3 cut(s) 306, 553, 694
AlwI GGATC 1 cut(s) 85
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 511
AoxI GGCC 3 cut(s) 362, 508, 932
ApeKI GCWGC 1 cut(s) 749
ApoI RAATTY 1 cut(s) 901
ArsI GACNNNNNNTTYG 2 cut(s) 664, 696
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 3 cut(s) 272, 509, 755
AsuHPI GGTGA 7 cut(s) 72, 177, 242, 416, 445, 778, 930
AsuII TTCGAA 1 cut(s) 797
AvaI CYCGRG 1 cut(s) 511
AvaII GGWCC 2 cut(s) 272, 755
BalI TGGCCA 1 cut(s) 934
BanI GGYRCC 1 cut(s) 220
BanII GRGCYC 1 cut(s) 520
BbsI GAAGAC 3 cut(s) 262, 477, 806
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 1 cut(s) 736
BccI CCATC 5 cut(s) 272, 322, 464, 701, 943
BciT130I CCWGG 3 cut(s) 84, 324, 369
BcoDI GTCTC 3 cut(s) 306, 553, 694
BfaI CTAG 1 cut(s) 699
BfmI CTRYAG 1 cut(s) 825
BfoI RGCGCY 1 cut(s) 224
BisI GCNGC 2 cut(s) 362, 750
BlsI GCNGC 2 cut(s) 363, 751
Bme1390I CCNGG 3 cut(s) 84, 324, 369
Bme18I GGWCC 2 cut(s) 272, 755
BmeRI GACNNNNNGTC 1 cut(s) 564
BmeT110I CYCGRG 1 cut(s) 511
BmgT120I GGNCC 3 cut(s) 272, 509, 755
BmiI GGNNCC 2 cut(s) 222, 661
BmrFI CCNGG 3 cut(s) 84, 324, 369
BmrI ACTGGG 1 cut(s) 57
BmsI GCATC 3 cut(s) 218, 404, 975
BmuI ACTGGG 1 cut(s) 57
BpiI GAAGAC 3 cut(s) 262, 477, 806
BpmI CTGGAG 1 cut(s) 646
Bpu14I TTCGAA 1 cut(s) 797
BpuEI CTTGAG 1 cut(s) 894
BsaHI GRCGYC 1 cut(s) 221
BsaJI CCNNGG 2 cut(s) 323, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 324, 350, 374
Bse1I ACTGG 3 cut(s) 52, 161, 679
Bse3DI GCAATG 3 cut(s) 12, 468, 474
BseBI CCWGG 3 cut(s) 84, 324, 369
BseDI CCNNGG 2 cut(s) 323, 368
BseGI GGATG 3 cut(s) 91, 311, 333
BseLI CCNNNNNNNGG 4 cut(s) 175, 324, 350, 374
BseMI GCAATG 3 cut(s) 12, 468, 474
BseNI ACTGG 3 cut(s) 52, 161, 679
BseRI GAGGAG 1 cut(s) 741
BseXI GCAGC 1 cut(s) 736
BshFI GGCC 3 cut(s) 364, 510, 934
BshNI GGYRCC 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 511
BslFI GGGAC 2 cut(s) 295, 618
BslI CCNNNNNNNGG 4 cut(s) 175, 324, 350, 374
BsmAI GTCTC 3 cut(s) 306, 553, 694
BsmFI GGGAC 2 cut(s) 295, 618
BsmI GAATGC 2 cut(s) 87, 744
BsnI GGCC 3 cut(s) 364, 510, 934
BsoBI CYCGRG 1 cut(s) 511
Bsp119I TTCGAA 1 cut(s) 797
Bsp1286I GDGCHC 1 cut(s) 520
Bsp1407I TGTACA 1 cut(s) 760
Bsp143I GATC 3 cut(s) 38, 77, 895
BspACI CCGC 1 cut(s) 361
BspANI GGCC 3 cut(s) 364, 510, 934
BspLI GGNNCC 2 cut(s) 222, 661
BspPI GGATC 1 cut(s) 85
BspT104I TTCGAA 1 cut(s) 797
BspT107I GGYRCC 1 cut(s) 220
BsrDI GCAATG 3 cut(s) 12, 468, 474
BsrGI TGTACA 1 cut(s) 760
BsrI ACTGG 3 cut(s) 52, 161, 679
BssECI CCNNGG 2 cut(s) 323, 368
BssMI GATC 3 cut(s) 38, 77, 895
BssNI GRCGYC 1 cut(s) 221
Bst2UI CCWGG 3 cut(s) 84, 324, 369
Bst4CI ACNGT 8 cut(s) 63, 115, 286, 556, 592, 609, 859, 965
Bst6I CTCTTC 1 cut(s) 567
BstACI GRCGYC 1 cut(s) 221
BstAUI TGTACA 1 cut(s) 760
BstBI TTCGAA 1 cut(s) 797
BstC8I GCNNGC 1 cut(s) 887
BstDEI CTNAG 1 cut(s) 683
BstF5I GGATG 3 cut(s) 91, 311, 333
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 3 cut(s) 41, 80, 898
BstMAI GTCTC 3 cut(s) 306, 553, 694
BstMBI GATC 3 cut(s) 38, 77, 895
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 3 cut(s) 84, 324, 369
BstNSI RCATGY 1 cut(s) 889
BstSCI CCNGG 3 cut(s) 82, 322, 367
BstSFI CTRYAG 1 cut(s) 825
BstV1I GCAGC 1 cut(s) 736
BstV2I GAAGAC 3 cut(s) 262, 477, 806
BstXI CCANNNNNNTGG 1 cut(s) 463
BsuRI GGCC 3 cut(s) 364, 510, 934
BtgZI GCGATG 1 cut(s) 442
BtsCI GGATG 3 cut(s) 91, 311, 333
BtsIMutI CAGTG 1 cut(s) 645
Cac8I GCNNGC 1 cut(s) 887
CaiI CAGNNNCTG 1 cut(s) 374
CfoI GCGC 1 cut(s) 223
Cfr13I GGNCC 3 cut(s) 272, 509, 755
Csp6I GTAC 2 cut(s) 141, 761
CspCI CAANNNNNGTGG 2 cut(s) 930, 965
CviAII CATG 3 cut(s) 191, 886, 968
CviQI GTAC 2 cut(s) 141, 761
DdeI CTNAG 1 cut(s) 683
DinI GGCGCC 1 cut(s) 222
DpnI GATC 3 cut(s) 40, 79, 897
DpnII GATC 3 cut(s) 38, 77, 895
DraIII CACNNNGTG 1 cut(s) 230
DriI GACNNNNNGTC 1 cut(s) 564
EaeI YGGCCR 2 cut(s) 362, 932
Eam1104I CTCTTC 1 cut(s) 567
Eam1105I GACNNNNNGTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 518
Eco24I GRGCYC 1 cut(s) 520
Eco47I GGWCC 2 cut(s) 272, 755
Eco53kI GAGCTC 1 cut(s) 518
Eco88I CYCGRG 1 cut(s) 511
EcoICRI GAGCTC 1 cut(s) 518
EcoRII CCWGG 3 cut(s) 82, 322, 367
EcoT38I GRGCYC 1 cut(s) 520
EgeI GGCGCC 1 cut(s) 222
EheI GGCGCC 1 cut(s) 222
FaeI CATG 3 cut(s) 194, 889, 971
FaqI GGGAC 2 cut(s) 295, 618
FatI CATG 3 cut(s) 190, 885, 967
FauNDI CATATG 2 cut(s) 598, 785
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 2 cut(s) 362, 750
FokI GGATG 3 cut(s) 78, 298, 340
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 2 cut(s) 362, 750
FspBI CTAG 1 cut(s) 699
GlaI GCGC 1 cut(s) 222
GluI GCNGC 2 cut(s) 362, 750
GsuI CTGGAG 1 cut(s) 646
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 3 cut(s) 364, 510, 934
HhaI GCGC 1 cut(s) 223
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 3 cut(s) 194, 889, 971
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HincII GTYRAC 3 cut(s) 153, 862, 973
HindII GTYRAC 3 cut(s) 153, 862, 973
HinfI GANTC 2 cut(s) 233, 559
HphI GGTGA 7 cut(s) 72, 177, 242, 416, 445, 778, 930
Hpy166II GTNNAC 4 cut(s) 153, 166, 862, 973
Hpy188III TCNNGA 3 cut(s) 54, 663, 704
Hpy8I GTNNAC 4 cut(s) 153, 166, 862, 973
HpyAV CCTTC 2 cut(s) 180, 824
HpyCH4III ACNGT 8 cut(s) 63, 115, 286, 556, 592, 609, 859, 965
HpyCH4V TGCA 1 cut(s) 209
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 1 cut(s) 683
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 3 cut(s) 194, 889, 971
HspAI GCGC 1 cut(s) 221
KasI GGCGCC 1 cut(s) 220
Kzo9I GATC 3 cut(s) 38, 77, 895
LmnI GCTCC 1 cut(s) 665
Lsp1109I GCAGC 1 cut(s) 736
LweI GCATC 3 cut(s) 218, 404, 975
MaeI CTAG 1 cut(s) 699
MaeIII GTNAC 6 cut(s) 69, 183, 556, 592, 835, 959
MalI GATC 3 cut(s) 40, 79, 897
MboI GATC 3 cut(s) 38, 77, 895
MboII GAAGA 6 cut(s) 165, 267, 391, 482, 554, 811
MhlI GDGCHC 1 cut(s) 520
MlsI TGGCCA 1 cut(s) 934
MluCI AATT 4 cut(s) 389, 618, 865, 901
MluNI TGGCCA 1 cut(s) 934
Mly113I GGCGCC 1 cut(s) 221
MlyI GAGTC 1 cut(s) 553
Mox20I TGGCCA 1 cut(s) 934
MscI TGGCCA 1 cut(s) 934
MseI TTAA 1 cut(s) 879
MslI CAYNNNNRTG 1 cut(s) 461
Msp20I TGGCCA 1 cut(s) 934
MspA1I CMGCKG 1 cut(s) 425
MspR9I CCNGG 3 cut(s) 84, 324, 369
Mva1269I GAATGC 2 cut(s) 87, 744
MvaI CCWGG 3 cut(s) 84, 324, 369
MwoI GCNNNNNNNGC 1 cut(s) 542
NarI GGCGCC 1 cut(s) 221
NdeI CATATG 2 cut(s) 598, 785
NdeII GATC 3 cut(s) 38, 77, 895
NlaIII CATG 3 cut(s) 194, 889, 971
NlaIV GGNNCC 2 cut(s) 222, 661
NmuCI GTSAC 4 cut(s) 183, 556, 592, 835
NspI RCATGY 1 cut(s) 889
NspV TTCGAA 1 cut(s) 797
PaeI GCATGC 1 cut(s) 889
PctI GAATGC 2 cut(s) 87, 744
PfeI GAWTC 1 cut(s) 233
PflMI CCANNNNNTGG 1 cut(s) 374
PkrI GCNGC 2 cut(s) 363, 751
PleI GAGTC 1 cut(s) 553
PluTI GGCGCC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 553
Psp124BI GAGCTC 1 cut(s) 520
Psp6I CCWGG 3 cut(s) 82, 322, 367
PspGI CCWGG 3 cut(s) 82, 322, 367
PspN4I GGNNCC 2 cut(s) 222, 661
PspPI GGNCC 3 cut(s) 272, 509, 755
PstNI CAGNNNCTG 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 425
RsaI GTAC 2 cut(s) 142, 762
RsaNI GTAC 2 cut(s) 141, 761
RseI CAYNNNNRTG 1 cut(s) 461
SacI GAGCTC 1 cut(s) 520
SaqAI TTAA 1 cut(s) 879
SatI GCNGC 2 cut(s) 362, 750
Sau3AI GATC 3 cut(s) 38, 77, 895
Sau96I GGNCC 3 cut(s) 272, 509, 755
SchI GAGTC 1 cut(s) 553
ScrFI CCNGG 3 cut(s) 84, 324, 369
SduI GDGCHC 1 cut(s) 520
SfaNI GCATC 3 cut(s) 218, 404, 975
SfcI CTRYAG 1 cut(s) 825
SfoI GGCGCC 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 797
SinI GGWCC 2 cut(s) 272, 755
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 909
SmoI CTYRAG 1 cut(s) 909
SphI GCATGC 1 cut(s) 889
Sse9I AATT 4 cut(s) 389, 618, 865, 901
SsiI CCGC 1 cut(s) 361
SspDI GGCGCC 1 cut(s) 220
SspMI CTAG 1 cut(s) 699
SstI GAGCTC 1 cut(s) 520
StyD4I CCNGG 3 cut(s) 82, 322, 367
TaaI ACNGT 8 cut(s) 63, 115, 286, 556, 592, 609, 859, 965
TaqI TCGA 4 cut(s) 262, 670, 797, 846
TasI AATT 4 cut(s) 389, 618, 865, 901
TatI WGTACW 1 cut(s) 760
TauI GCSGC 1 cut(s) 364
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 1 cut(s) 879
Tru9I TTAA 1 cut(s) 879
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 4 cut(s) 183, 556, 592, 835
TseI GCWGC 1 cut(s) 749
Tsp45I GTSAC 4 cut(s) 183, 556, 592, 835
TspDTI ATGAA 3 cut(s) 80, 279, 772
TspGWI ACGGA 1 cut(s) 294
TspRI CASTG 1 cut(s) 652
Van91I CCANNNNNTGG 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 272, 755
XapI RAATTY 1 cut(s) 901
XceI RCATGY 1 cut(s) 889
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.