Rh1BG225300

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0009833
Physical Location & Seq
Reverse (-)
34543481 .. 34548593
5113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG225300.1

Sequence Viewer

Length: 1014 bp
ATGGATCGGAGGGACCATATGGCAATGTCGGGGTCAGCCTCTTATTACACGCCGAGATCAATAACTGGGTCTGGAACACAGTCTGGGTTACTTGGATCACCAGGCATTCATCCCTTATCTAATCCAAATGCAGTATTTCAGTCCAATATGGGGGCCGGTACGATTGGGTCAACATTACCAGTAGACCCTTCTTCGGCTATTTCACCTCATAGTGTCAATGTGGGTGCATCGTCTGTGGCGCCACAAGGTGAATCTGTGAAAAGGAAAAGAGGAAGACCTCGAAAATATGGACCTGATGGGACTGTTTCATTGGCATTATCTCCGTCATCATCCTCCAATCCTGGGATGGTAGCTTCAAGCCCAAAGCGAGGTAGAGGGAGGCCACCAGGGTCTGGGAAGAAGCACCAATTAGCGTCTCTTGGTGAAATGCTATCTGGTTCAGCTGGAATGGGTTTCACTCCACATATTATCACCATCGCAATGGGAGAAGACATTGCAACAAAAATAATGGCATTTTCACAGCAAGGCCCGAGAGCTCTCTGCGTCTTATCAGCCAATGGTGCTGTCTCCACTGTGACTCTTCGTCAGCCCTCCACTTCTGGTGGCACAGTCACATATGAGGGACGGTTTGAGATAATTTGTCTGTCAGGCTCTTACTTGCTCACTGAAAGTGGTGGCTCCAGAAATCGAACTGGTGGTCTTAGTGTCTCCCTTGCTAGTCCTGATGGTCGTGTTGTTGGTGGAGGAGTAGGAGGAATGCTTATTGCTGCTGGCCCTGTACAGGTGATAGTGGGTAGCTTCATATGGGGTGGTTCAAAGACAAAGAACAAGAAAAAAGAAGCTATAGAAGGTGTCACAGACTTCGACCAACAGACAGTTGACAATTCAGTTCCATTTAATAGCATGCCACCCGATCAAAATTTTACTCAAGGGTCATCGTTGGGCGTGTGGCCATCACCACGACCATTGGATATGCATAACAGTCATGTTGACATTGATTTGATGCGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

337

Amino Acids

34.65

Weight (kDa)

9.76

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 153 - 267 1.3e-25 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AccB7I CCANNNNNTGG 1 cut(s) 392
AccI GTMKAC 1 cut(s) 183
AclWI GGATC 2 cut(s) 12, 103
AcoI YGGCCR 1 cut(s) 950
AcsI RAATTY 1 cut(s) 919
AcyI GRCGYC 1 cut(s) 239
AdeI CACNNNGTG 1 cut(s) 248
AfaI GTAC 2 cut(s) 160, 780
AfiI CCNNNNNNNGG 6 cut(s) 150, 193, 342, 368, 392, 781
AgsI TTSAA 2 cut(s) 357, 816
AhdI GACNNNNNGTC 1 cut(s) 582
AjnI CCWGG 3 cut(s) 100, 340, 385
AluBI AGCT 5 cut(s) 353, 443, 536, 798, 842
AluI AGCT 5 cut(s) 353, 443, 536, 798, 842
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 3 cut(s) 420, 571, 712
AlwI GGATC 2 cut(s) 12, 103
AlwNI CAGNNNCTG 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 529
AoxI GGCC 5 cut(s) 153, 380, 526, 772, 950
ApeKI GCWGC 1 cut(s) 767
ApoI RAATTY 1 cut(s) 919
ArsI GACNNNNNNTTYG 2 cut(s) 682, 714
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 5 cut(s) 13, 153, 290, 527, 773
AsuHPI GGTGA 7 cut(s) 90, 195, 260, 434, 463, 796, 948
AvaI CYCGRG 1 cut(s) 529
AvaII GGWCC 2 cut(s) 13, 290
BalI TGGCCA 1 cut(s) 952
BanI GGYRCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 538
BbsI GAAGAC 2 cut(s) 280, 495
Bbv12I GWGCWC 1 cut(s) 538
BbvI GCAGC 1 cut(s) 754
BccI CCATC 5 cut(s) 290, 340, 482, 719, 961
BciT130I CCWGG 3 cut(s) 102, 342, 387
BcoDI GTCTC 3 cut(s) 420, 571, 712
BfaI CTAG 1 cut(s) 717
BfmI CTRYAG 1 cut(s) 843
BfoI RGCGCY 1 cut(s) 242
BisI GCNGC 1 cut(s) 768
BlsI GCNGC 1 cut(s) 769
Bme1390I CCNGG 3 cut(s) 102, 342, 387
Bme18I GGWCC 2 cut(s) 13, 290
BmeRI GACNNNNNGTC 1 cut(s) 582
BmeT110I CYCGRG 1 cut(s) 529
BmgT120I GGNCC 5 cut(s) 13, 153, 290, 527, 773
BmiI GGNNCC 4 cut(s) 14, 154, 240, 679
BmrFI CCNGG 3 cut(s) 102, 342, 387
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 2 cut(s) 236, 993
BmuI ACTGGG 1 cut(s) 75
BpiI GAAGAC 2 cut(s) 280, 495
BpmI CTGGAG 1 cut(s) 664
BpuEI CTTGAG 1 cut(s) 912
BsaHI GRCGYC 1 cut(s) 239
BsaJI CCNNGG 2 cut(s) 341, 386
Bsc4I CCNNNNNNNGG 6 cut(s) 150, 193, 342, 368, 392, 781
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 3 cut(s) 70, 179, 697
Bse3DI GCAATG 3 cut(s) 30, 486, 492
BseBI CCWGG 3 cut(s) 102, 342, 387
BseDI CCNNGG 2 cut(s) 341, 386
BseGI GGATG 3 cut(s) 109, 329, 351
BseLI CCNNNNNNNGG 6 cut(s) 150, 193, 342, 368, 392, 781
BseMI GCAATG 3 cut(s) 30, 486, 492
BseNI ACTGG 3 cut(s) 70, 179, 697
BseRI GAGGAG 1 cut(s) 759
BseXI GCAGC 1 cut(s) 754
BshFI GGCC 5 cut(s) 155, 382, 528, 774, 952
BshNI GGYRCC 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 538
BsiHKCI CYCGRG 1 cut(s) 529
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 3 cut(s) 26, 313, 636
BslI CCNNNNNNNGG 6 cut(s) 150, 193, 342, 368, 392, 781
BsmAI GTCTC 3 cut(s) 420, 571, 712
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 3 cut(s) 26, 313, 636
BsmI GAATGC 2 cut(s) 105, 762
BsnI GGCC 5 cut(s) 155, 382, 528, 774, 952
BsoBI CYCGRG 1 cut(s) 529
Bsp1286I GDGCHC 1 cut(s) 538
Bsp1407I TGTACA 1 cut(s) 778
Bsp143I GATC 4 cut(s) 4, 56, 95, 913
BspANI GGCC 5 cut(s) 155, 382, 528, 774, 952
BspLI GGNNCC 4 cut(s) 14, 154, 240, 679
BspPI GGATC 2 cut(s) 12, 103
BspT107I GGYRCC 1 cut(s) 238
BsrDI GCAATG 3 cut(s) 30, 486, 492
BsrFI RCCGGY 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 778
BsrI ACTGG 3 cut(s) 70, 179, 697
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 341, 386
BssMI GATC 4 cut(s) 4, 56, 95, 913
BssNI GRCGYC 1 cut(s) 239
Bst2UI CCWGG 3 cut(s) 102, 342, 387
Bst4CI ACNGT 7 cut(s) 81, 304, 574, 610, 627, 877, 983
Bst6I CTCTTC 1 cut(s) 585
BstACI GRCGYC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 778
BstC8I GCNNGC 2 cut(s) 772, 905
BstDEI CTNAG 1 cut(s) 701
BstF5I GGATG 3 cut(s) 109, 329, 351
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 4 cut(s) 7, 59, 98, 916
BstMAI GTCTC 3 cut(s) 420, 571, 712
BstMBI GATC 4 cut(s) 4, 56, 95, 913
BstMWI GCNNNNNNNGC 1 cut(s) 560
BstNI CCWGG 3 cut(s) 102, 342, 387
BstNSI RCATGY 1 cut(s) 907
BstSCI CCNGG 3 cut(s) 100, 340, 385
BstSFI CTRYAG 1 cut(s) 843
BstV1I GCAGC 1 cut(s) 754
BstV2I GAAGAC 2 cut(s) 280, 495
BstXI CCANNNNNNTGG 1 cut(s) 481
BsuRI GGCC 5 cut(s) 155, 382, 528, 774, 952
BtgZI GCGATG 1 cut(s) 460
BtsCI GGATG 3 cut(s) 109, 329, 351
BtsIMutI CAGTG 2 cut(s) 570, 663
Cac8I GCNNGC 2 cut(s) 772, 905
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 5 cut(s) 13, 153, 290, 527, 773
CseI GACGC 2 cut(s) 402, 532
Csp6I GTAC 2 cut(s) 159, 779
CspCI CAANNNNNGTGG 2 cut(s) 948, 983
CviAII CATG 2 cut(s) 904, 986
CviQI GTAC 2 cut(s) 159, 779
DdeI CTNAG 1 cut(s) 701
DinI GGCGCC 1 cut(s) 240
DpnI GATC 4 cut(s) 6, 58, 97, 915
DpnII GATC 4 cut(s) 4, 56, 95, 913
DraIII CACNNNGTG 1 cut(s) 248
DriI GACNNNNNGTC 1 cut(s) 582
EaeI YGGCCR 1 cut(s) 950
Eam1104I CTCTTC 1 cut(s) 585
Eam1105I GACNNNNNGTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 585
Ecl136II GAGCTC 1 cut(s) 536
Eco24I GRGCYC 1 cut(s) 538
Eco47I GGWCC 2 cut(s) 13, 290
Eco53kI GAGCTC 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 529
EcoICRI GAGCTC 1 cut(s) 536
EcoRII CCWGG 3 cut(s) 100, 340, 385
EcoT22I ATGCAT 1 cut(s) 978
EcoT38I GRGCYC 1 cut(s) 538
EgeI GGCGCC 1 cut(s) 240
EheI GGCGCC 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 420
FaeI CATG 2 cut(s) 907, 989
FaqI GGGAC 3 cut(s) 26, 313, 636
FatI CATG 2 cut(s) 903, 985
FauNDI CATATG 3 cut(s) 18, 616, 803
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 1 cut(s) 768
FokI GGATG 3 cut(s) 96, 316, 358
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 1 cut(s) 768
FspBI CTAG 1 cut(s) 717
GlaI GCGC 1 cut(s) 240
GluI GCNGC 1 cut(s) 768
GsuI CTGGAG 1 cut(s) 664
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 5 cut(s) 155, 382, 528, 774, 952
HapII CCGG 1 cut(s) 156
HgaI GACGC 2 cut(s) 402, 532
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 239
Hin1II CATG 2 cut(s) 907, 989
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 3 cut(s) 171, 880, 991
HindII GTYRAC 3 cut(s) 171, 880, 991
HinfI GANTC 2 cut(s) 251, 577
HpaII CCGG 1 cut(s) 156
HphI GGTGA 7 cut(s) 90, 195, 260, 434, 463, 796, 948
Hpy166II GTNNAC 4 cut(s) 171, 184, 880, 991
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 3 cut(s) 72, 681, 722
Hpy8I GTNNAC 4 cut(s) 171, 184, 880, 991
HpyAV CCTTC 2 cut(s) 198, 842
HpyCH4III ACNGT 7 cut(s) 81, 304, 574, 610, 627, 877, 983
HpyCH4V TGCA 4 cut(s) 131, 227, 497, 976
HpyF10VI GCNNNNNNNGC 1 cut(s) 560
HpyF3I CTNAG 1 cut(s) 701
Hsp92I GRCGYC 1 cut(s) 239
Hsp92II CATG 2 cut(s) 907, 989
HspAI GCGC 1 cut(s) 239
KasI GGCGCC 1 cut(s) 238
Kzo9I GATC 4 cut(s) 4, 56, 95, 913
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 1 cut(s) 754
LweI GCATC 2 cut(s) 236, 993
MaeI CTAG 1 cut(s) 717
MaeIII GTNAC 4 cut(s) 87, 574, 610, 853
MalI GATC 4 cut(s) 6, 58, 97, 915
MboI GATC 4 cut(s) 4, 56, 95, 913
MboII GAAGA 5 cut(s) 183, 285, 409, 500, 572
MhlI GDGCHC 1 cut(s) 538
MlsI TGGCCA 1 cut(s) 952
MluCI AATT 4 cut(s) 407, 636, 883, 919
MluNI TGGCCA 1 cut(s) 952
Mly113I GGCGCC 1 cut(s) 239
MlyI GAGTC 1 cut(s) 571
Mox20I TGGCCA 1 cut(s) 952
Mph1103I ATGCAT 1 cut(s) 978
MscI TGGCCA 1 cut(s) 952
MseI TTAA 1 cut(s) 897
MslI CAYNNNNRTG 1 cut(s) 479
Msp20I TGGCCA 1 cut(s) 952
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 3 cut(s) 102, 342, 387
Mva1269I GAATGC 2 cut(s) 105, 762
MvaI CCWGG 3 cut(s) 102, 342, 387
MwoI GCNNNNNNNGC 1 cut(s) 560
NarI GGCGCC 1 cut(s) 239
NdeI CATATG 3 cut(s) 18, 616, 803
NdeII GATC 4 cut(s) 4, 56, 95, 913
NlaIII CATG 2 cut(s) 907, 989
NlaIV GGNNCC 4 cut(s) 14, 154, 240, 679
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 3 cut(s) 574, 610, 853
NsiI ATGCAT 1 cut(s) 978
NspI RCATGY 1 cut(s) 907
PaeI GCATGC 1 cut(s) 907
PctI GAATGC 2 cut(s) 105, 762
PfeI GAWTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 1 cut(s) 769
PleI GAGTC 1 cut(s) 571
PluTI GGCGCC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 571
Psp124BI GAGCTC 1 cut(s) 538
Psp6I CCWGG 3 cut(s) 100, 340, 385
PspGI CCWGG 3 cut(s) 100, 340, 385
PspN4I GGNNCC 4 cut(s) 14, 154, 240, 679
PspPI GGNCC 5 cut(s) 13, 153, 290, 527, 773
PstNI CAGNNNCTG 1 cut(s) 392
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 2 cut(s) 160, 780
RsaNI GTAC 2 cut(s) 159, 779
RseI CAYNNNNRTG 1 cut(s) 479
SacI GAGCTC 1 cut(s) 538
SaqAI TTAA 1 cut(s) 897
SatI GCNGC 1 cut(s) 768
Sau3AI GATC 4 cut(s) 4, 56, 95, 913
Sau96I GGNCC 5 cut(s) 13, 153, 290, 527, 773
SchI GAGTC 1 cut(s) 571
ScrFI CCNGG 3 cut(s) 102, 342, 387
SduI GDGCHC 1 cut(s) 538
SfaNI GCATC 2 cut(s) 236, 993
SfcI CTRYAG 1 cut(s) 843
SfoI GGCGCC 1 cut(s) 240
SinI GGWCC 2 cut(s) 13, 290
SmiMI CAYNNNNRTG 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 927
SmoI CTYRAG 1 cut(s) 927
SphI GCATGC 1 cut(s) 907
Sse9I AATT 4 cut(s) 407, 636, 883, 919
SspDI GGCGCC 1 cut(s) 238
SspMI CTAG 1 cut(s) 717
SstI GAGCTC 1 cut(s) 538
StyD4I CCNGG 3 cut(s) 100, 340, 385
TaaI ACNGT 7 cut(s) 81, 304, 574, 610, 627, 877, 983
TaqI TCGA 3 cut(s) 280, 688, 864
TasI AATT 4 cut(s) 407, 636, 883, 919
TatI WGTACW 1 cut(s) 778
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 897
Tru9I TTAA 1 cut(s) 897
TscAI CASTG 2 cut(s) 577, 670
TseFI GTSAC 3 cut(s) 574, 610, 853
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 3 cut(s) 574, 610, 853
TspDTI ATGAA 3 cut(s) 98, 297, 790
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 2 cut(s) 577, 670
Van91I CCANNNNNTGG 1 cut(s) 392
VpaK11BI GGWCC 2 cut(s) 13, 290
XapI RAATTY 1 cut(s) 919
XceI RCATGY 1 cut(s) 907
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 1 cut(s) 717
Zsp2I ATGCAT 1 cut(s) 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.