MD07G1139600.v1.1

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009833
Physical Location & Seq
Forward (+)
20211802 .. 20216074
4273 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1139600.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGGATCGGAGGGACCCTATGGCATTGTCGGGGTCAGCCTCTTACTTTACGTCTAGAGGAATAACTGGTTCCGGAACACAATCTGGGATACATGGATCACCAGGCATTCATCCCTTATCTAATACAAATATAGCATTTCAATCCAATATTGAAGGAAGTAACATTGGATCAACATTGCCGGTAGGGCCTTCTTCGGCTATTTCACAGCATGGTGTCAATCTGGGTGCACCATCCGCGGTGCCACCAGGCGAATCTTTTAAAAGGAAAAGAGGAAGACCTCGAAAATATGGACCTGATGGGACTGTTTCATTGGCATTGTCTCCTGCAGCATCTGCTAATCCTGGTACAGTATCATCAAGCCCAAAACGGGGTAGAGGGCGGCCACCAGGGTCTGGGAAGAAGCAACAATTAGTGTCTCATGGCGGATTGCTGTCTGGTTCTGCTGGGATGGGATTTACTCCACATATTATCAGCATTGCAGTGGGAGAAGATGTTGCAACAAAAATAATGGCGTTTTCGCAGCAGGGGCCAAGAGCTGTGTGCATCTTGTCAGCCAACGGTGCTGTCTCTACTGTGACGCTACGTCAGCCCTCAACTTCTGGTGGTACAGTCACATATGAGGGACGGTTTGAGATAATATGTCTCTCAGGCTCTTACTTGCTCACTGAAATTGGTGGTTCCCGAAACCGAAATGGCGGTCTGAGCGTCTCCCTTGCTAGTCCTGATGGTCGTGTCATTGGTGGAGGAGTAGGAGGGATGCTTATTGCAGCAACCCCTGTTCAGGTGATAGTAGGTAGCTTCATATGGGGTGGTTCGAAGACAAAGAACAAGAAAAGGGAAGCCGTAGAAGGTGTCACAGACTTGGAACACGAGACGGTTGACAATTCGGTTGCACTAAACAGCATTCAACCAGATCAGAGTGTAAGCCAAAGTGCTTCATTGGCCGCGTGGCAGGCATCACGGCCACTGGATATGCGTAACAGTCATGTTGACATTGATTTGATGCGAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

338

Amino Acids

34.47

Weight (kDa)

9.95

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 153 - 267 1.5e-25 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AccB7I CCANNNNNTGG 1 cut(s) 392
AccII CGCG 2 cut(s) 236, 947
AccIII TCCGGA 1 cut(s) 71
AciI CCGC 6 cut(s) 234, 236, 379, 423, 696, 945
AclWI GGATC 3 cut(s) 12, 103, 175
AcoI YGGCCR 3 cut(s) 380, 942, 962
AfaI GTAC 2 cut(s) 346, 607
AfiI CCNNNNNNNGG 4 cut(s) 367, 368, 392, 781
AgsI TTSAA 3 cut(s) 140, 152, 908
AhdI GACNNNNNGTC 1 cut(s) 582
AjnI CCWGG 4 cut(s) 100, 244, 340, 385
AluBI AGCT 2 cut(s) 536, 798
AluI AGCT 2 cut(s) 536, 798
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 6 cut(s) 324, 420, 571, 647, 712, 866
Alw44I GTGCAC 1 cut(s) 225
AlwI GGATC 3 cut(s) 12, 103, 175
AlwNI CAGNNNCTG 2 cut(s) 332, 392
Aor13HI TCCGGA 1 cut(s) 71
AoxI GGCC 5 cut(s) 185, 380, 527, 942, 962
ApaLI GTGCAC 1 cut(s) 225
ApeKI GCWGC 3 cut(s) 326, 520, 767
ArsI GACNNNNNNTTYG 2 cut(s) 682, 714
AspS9I GGNCC 4 cut(s) 13, 185, 290, 527
AsuHPI GGTGA 2 cut(s) 90, 796
AsuII TTCGAA 1 cut(s) 815
AvaII GGWCC 2 cut(s) 13, 290
BaeGI GKGCMC 1 cut(s) 229
BanI GGYRCC 1 cut(s) 238
BauI CACGAG 1 cut(s) 869
BbsI GAAGAC 2 cut(s) 280, 824
Bbv12I GWGCWC 1 cut(s) 229
BbvI GCAGC 3 cut(s) 338, 532, 779
BccI CCATC 4 cut(s) 238, 290, 442, 719
BceAI ACGGC 2 cut(s) 827, 977
BciT130I CCWGG 4 cut(s) 102, 246, 342, 387
BciVI GTATCC 1 cut(s) 81
BcoDI GTCTC 6 cut(s) 324, 420, 571, 647, 712, 866
BfaI CTAG 2 cut(s) 54, 717
BfmI CTRYAG 1 cut(s) 324
BfuI GTATCC 1 cut(s) 81
BglI GCCNNNNNGGC 1 cut(s) 184
BisI GCNGC 5 cut(s) 327, 380, 521, 768, 945
BlsI GCNGC 5 cut(s) 328, 381, 522, 769, 946
Bme1390I CCNGG 4 cut(s) 102, 246, 342, 387
Bme18I GGWCC 2 cut(s) 13, 290
BmeRI GACNNNNNGTC 1 cut(s) 582
BmgT120I GGNCC 4 cut(s) 13, 185, 290, 527
BmiI GGNNCC 6 cut(s) 14, 15, 70, 240, 528, 679
BmrFI CCNGG 4 cut(s) 102, 246, 342, 387
BmsI GCATC 5 cut(s) 338, 552, 747, 965, 993
BpiI GAAGAC 2 cut(s) 280, 824
Bpu14I TTCGAA 1 cut(s) 815
BsaJI CCNNGG 2 cut(s) 234, 386
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 4 cut(s) 367, 368, 392, 781
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 70, 972
Bse3DI GCAATG 2 cut(s) 173, 474
BseAI TCCGGA 1 cut(s) 71
BseBI CCWGG 4 cut(s) 102, 246, 342, 387
BseDI CCNNGG 2 cut(s) 234, 386
BseGI GGATG 4 cut(s) 109, 230, 453, 762
BseLI CCNNNNNNNGG 4 cut(s) 367, 368, 392, 781
BseMI GCAATG 2 cut(s) 173, 474
BseMII CTCAG 2 cut(s) 660, 692
BseNI ACTGG 2 cut(s) 70, 972
BseRI GAGGAG 1 cut(s) 759
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 3 cut(s) 338, 532, 779
BseYI CCCAGC 1 cut(s) 443
Bsh1236I CGCG 2 cut(s) 236, 947
BshFI GGCC 5 cut(s) 187, 382, 529, 944, 964
BshNI GGYRCC 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 229
BsiSI CCGG 2 cut(s) 72, 179
BslFI GGGAC 3 cut(s) 26, 313, 636
BslI CCNNNNNNNGG 4 cut(s) 367, 368, 392, 781
BsmAI GTCTC 6 cut(s) 324, 420, 571, 647, 712, 866
BsmBI CGTCTC 2 cut(s) 712, 866
BsmFI GGGAC 3 cut(s) 26, 313, 636
BsmI GAATGC 2 cut(s) 105, 903
BsnI GGCC 5 cut(s) 187, 382, 529, 944, 964
Bsp119I TTCGAA 1 cut(s) 815
Bsp1286I GDGCHC 1 cut(s) 229
Bsp13I TCCGGA 1 cut(s) 71
Bsp143I GATC 4 cut(s) 4, 95, 167, 913
BspACI CCGC 6 cut(s) 234, 236, 379, 423, 696, 945
BspANI GGCC 5 cut(s) 187, 382, 529, 944, 964
BspCNI CTCAG 2 cut(s) 659, 693
BspEI TCCGGA 1 cut(s) 71
BspFNI CGCG 2 cut(s) 236, 947
BspLI GGNNCC 6 cut(s) 14, 15, 70, 240, 528, 679
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 3 cut(s) 12, 103, 175
BspT104I TTCGAA 1 cut(s) 815
BspT107I GGYRCC 1 cut(s) 238
BsrDI GCAATG 2 cut(s) 173, 474
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 2 cut(s) 70, 972
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 234, 386
BssMI GATC 4 cut(s) 4, 95, 167, 913
BssSI CACGAG 1 cut(s) 869
Bst2BI CACGAG 1 cut(s) 869
Bst2UI CCWGG 4 cut(s) 102, 246, 342, 387
Bst4CI ACNGT 8 cut(s) 304, 349, 560, 574, 610, 627, 877, 983
BstAPI GCANNNNNTGC 1 cut(s) 332
BstBI TTCGAA 1 cut(s) 815
BstC8I GCNNGC 1 cut(s) 954
BstDEI CTNAG 2 cut(s) 646, 701
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 4 cut(s) 109, 230, 453, 762
BstFNI CGCG 2 cut(s) 236, 947
BstKTI GATC 4 cut(s) 7, 98, 170, 916
BstMAI GTCTC 6 cut(s) 324, 420, 571, 647, 712, 866
BstMBI GATC 4 cut(s) 4, 95, 167, 913
BstMWI GCNNNNNNNGC 9 cut(s) 184, 233, 332, 526, 560, 586, 702, 941, 953
BstNI CCWGG 4 cut(s) 102, 246, 342, 387
BstSCI CCNGG 4 cut(s) 100, 244, 340, 385
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 229
BstUI CGCG 2 cut(s) 236, 947
BstV1I GCAGC 3 cut(s) 338, 532, 779
BstV2I GAAGAC 2 cut(s) 280, 824
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 5 cut(s) 187, 382, 529, 944, 964
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 4 cut(s) 109, 230, 453, 762
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 3 cut(s) 486, 663, 965
Cac8I GCNNGC 1 cut(s) 954
CaiI CAGNNNCTG 2 cut(s) 332, 392
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 4 cut(s) 13, 185, 290, 527
Cfr42I CCGCGG 1 cut(s) 237
CseI GACGC 2 cut(s) 586, 694
Csp6I GTAC 2 cut(s) 345, 606
CviAII CATG 4 cut(s) 92, 209, 419, 986
CviQI GTAC 2 cut(s) 345, 606
DdeI CTNAG 2 cut(s) 646, 701
DpnI GATC 4 cut(s) 6, 97, 169, 915
DpnII GATC 4 cut(s) 4, 95, 167, 913
DraI TTTAAA 1 cut(s) 259
DriI GACNNNNNGTC 1 cut(s) 582
EaeI YGGCCR 3 cut(s) 380, 942, 962
Eam1105I GACNNNNNGTC 1 cut(s) 582
EciI GGCGGA 1 cut(s) 438
Eco47I GGWCC 2 cut(s) 13, 290
EcoO109I RGGNCCY 2 cut(s) 13, 185
EcoRII CCWGG 4 cut(s) 100, 244, 340, 385
Esp3I CGTCTC 2 cut(s) 712, 866
FaeI CATG 4 cut(s) 95, 212, 422, 989
FaqI GGGAC 3 cut(s) 26, 313, 636
FatI CATG 4 cut(s) 91, 208, 418, 985
FauNDI CATATG 2 cut(s) 616, 803
Fnu4HI GCNGC 5 cut(s) 327, 380, 521, 768, 945
FokI GGATG 4 cut(s) 96, 217, 460, 769
Fsp4HI GCNGC 5 cut(s) 327, 380, 521, 768, 945
FspBI CTAG 2 cut(s) 54, 717
GluI GCNGC 5 cut(s) 327, 380, 521, 768, 945
GsaI CCCAGC 1 cut(s) 447
HaeIII GGCC 5 cut(s) 187, 382, 529, 944, 964
HapII CCGG 2 cut(s) 72, 179
HgaI GACGC 2 cut(s) 586, 694
Hin1II CATG 4 cut(s) 95, 212, 422, 989
HincII GTYRAC 2 cut(s) 880, 991
HindII GTYRAC 2 cut(s) 880, 991
HinfI GANTC 1 cut(s) 251
HpaII CCGG 2 cut(s) 72, 179
HphI GGTGA 2 cut(s) 90, 796
Hpy166II GTNNAC 3 cut(s) 227, 880, 991
Hpy188I TCNGA 3 cut(s) 9, 702, 918
Hpy188III TCNNGA 4 cut(s) 54, 72, 681, 722
Hpy8I GTNNAC 3 cut(s) 227, 880, 991
HpyAV CCTTC 3 cut(s) 146, 198, 842
HpyCH4III ACNGT 8 cut(s) 304, 349, 560, 574, 610, 627, 877, 983
HpyCH4IV ACGT 2 cut(s) 50, 583
HpyCH4V TGCA 7 cut(s) 227, 326, 479, 497, 543, 767, 893
HpyF10VI GCNNNNNNNGC 9 cut(s) 184, 233, 332, 526, 560, 586, 702, 941, 953
HpyF3I CTNAG 2 cut(s) 646, 701
HpySE526I ACGT 2 cut(s) 50, 583
Hsp92II CATG 4 cut(s) 95, 212, 422, 989
KflI GGGWCCC 1 cut(s) 13
Kpn2I TCCGGA 1 cut(s) 71
KspI CCGCGG 1 cut(s) 237
Kzo9I GATC 4 cut(s) 4, 95, 167, 913
Lsp1109I GCAGC 3 cut(s) 338, 532, 779
LweI GCATC 5 cut(s) 338, 552, 747, 965, 993
MaeI CTAG 2 cut(s) 54, 717
MaeII ACGT 2 cut(s) 50, 583
MaeIII GTNAC 5 cut(s) 158, 574, 610, 853, 977
MalI GATC 4 cut(s) 6, 97, 169, 915
MboI GATC 4 cut(s) 4, 95, 167, 913
MboII GAAGA 5 cut(s) 183, 285, 409, 500, 829
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 3 cut(s) 407, 669, 883
MroI TCCGGA 1 cut(s) 71
MseI TTAA 1 cut(s) 258
MslI CAYNNNNRTG 1 cut(s) 479
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 2 cut(s) 72, 179
MspR9I CCNGG 4 cut(s) 102, 246, 342, 387
Mva1269I GAATGC 2 cut(s) 105, 903
MvaI CCWGG 4 cut(s) 102, 246, 342, 387
MvnI CGCG 2 cut(s) 236, 947
MwoI GCNNNNNNNGC 9 cut(s) 184, 233, 332, 526, 560, 586, 702, 941, 953
NdeI CATATG 2 cut(s) 616, 803
NdeII GATC 4 cut(s) 4, 95, 167, 913
NlaIII CATG 4 cut(s) 95, 212, 422, 989
NlaIV GGNNCC 6 cut(s) 14, 15, 70, 240, 528, 679
NmuCI GTSAC 3 cut(s) 574, 610, 853
NspV TTCGAA 1 cut(s) 815
PctI GAATGC 2 cut(s) 105, 903
PfeI GAWTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 5 cut(s) 328, 381, 522, 769, 946
PpuMI RGGWCCY 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 4 cut(s) 100, 244, 340, 385
PspFI CCCAGC 1 cut(s) 443
PspGI CCWGG 4 cut(s) 100, 244, 340, 385
PspN4I GGNNCC 6 cut(s) 14, 15, 70, 240, 528, 679
PspPI GGNCC 4 cut(s) 13, 185, 290, 527
PspPPI RGGWCCY 1 cut(s) 13
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 2 cut(s) 332, 392
RsaI GTAC 2 cut(s) 346, 607
RsaNI GTAC 2 cut(s) 345, 606
RseI CAYNNNNRTG 1 cut(s) 479
SacII CCGCGG 1 cut(s) 237
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 5 cut(s) 327, 380, 521, 768, 945
Sau3AI GATC 4 cut(s) 4, 95, 167, 913
Sau96I GGNCC 4 cut(s) 13, 185, 290, 527
ScrFI CCNGG 4 cut(s) 102, 246, 342, 387
SduI GDGCHC 1 cut(s) 229
SetI ASST 9 cut(s) 53, 280, 295, 538, 586, 786, 796, 800, 853
SfaNI GCATC 5 cut(s) 338, 552, 747, 965, 993
SfcI CTRYAG 1 cut(s) 324
Sfr303I CCGCGG 1 cut(s) 237
SfuI TTCGAA 1 cut(s) 815
SgrBI CCGCGG 1 cut(s) 237
SinI GGWCC 2 cut(s) 13, 290
SmiMI CAYNNNNRTG 1 cut(s) 479
Sse9I AATT 3 cut(s) 407, 669, 883
SsiI CCGC 6 cut(s) 234, 236, 379, 423, 696, 945
SspI AATATT 1 cut(s) 148
SspMI CTAG 2 cut(s) 54, 717
StyD4I CCNGG 4 cut(s) 100, 244, 340, 385
TaaI ACNGT 8 cut(s) 304, 349, 560, 574, 610, 627, 877, 983
TaiI ACGT 2 cut(s) 53, 586
TaqI TCGA 2 cut(s) 280, 815
TasI AATT 3 cut(s) 407, 669, 883
TauI GCSGC 2 cut(s) 382, 947
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 3 cut(s) 486, 670, 972
TseFI GTSAC 3 cut(s) 574, 610, 853
TseI GCWGC 3 cut(s) 326, 520, 767
Tsp45I GTSAC 3 cut(s) 574, 610, 853
TspDTI ATGAA 4 cut(s) 98, 297, 790, 927
TspRI CASTG 3 cut(s) 486, 670, 972
Van91I CCANNNNNTGG 1 cut(s) 392
VneI GTGCAC 1 cut(s) 225
VpaK11BI GGWCC 2 cut(s) 13, 290
XbaI TCTAGA 1 cut(s) 53
XspI CTAG 2 cut(s) 54, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.