Rroxscaffold_4G00300890

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009833
Physical Location & Seq
Forward (+)
21271821 .. 21276754
4934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00300890.1

Sequence Viewer

Length: 846 bp
ATGGATCGGAGGGACCATATGGCAATGTCGGGGTCAGCCTCTTATTACACTCCGAGATCAATAACTGGGTCTGGAACACAGTCTGGGTTACTTGGATCACCAGGCATTCATCCCTTATCTAATCCAAATGCAGTATTTCAGTCCAATATGGGGGCCAGTACGATTGGGTCAACATTACCAGTAGACCCTTCTTCGGCTATTTCACTTCATGGTGTCAATGTGGGTGCACCGTCTGTGGCGCCACAAGGTGAATCTGTGAAAAGGAAAAGAGGAAGACCTCGAAAATATGGACCTGATGGGACTGTTTCATTGGCATTATCTCCGTCATCATCCTCCAATCCTGGGATGGTAGCTTCAAGCCCAAAGCGAGGTAGAGGGCGGCCACCAGGGTCTGGGAAGAAGCACCAATTAGCGTCTCTTGGTGAAATGCTGTCTAGTTCAGCTGGAATGGGTTTCACTCCACATATTATCACCATCGCAATGGGAGAAGACATTGCAACAAAAATAATGGCATTTTCACAGCAAGGCCCGAGAGCTCTCTGCGTCTTATCAGCCAATGGTGCTGTCTCTACTGTGACTCTTCGTCAGCCCTCCACTTCTGGTGGCACAGTCACATATGAGGGACGGTTTGAGATAATTTGTCTATCAGGCTCTTACTTGCTCACTGAAAGTGGTGGCTCCAGAAATCGAACTGGTGGTCTTAGTGTCTCCCTTGCTAGTCCTGATGGTCGTGTTGTTGGTGGAGGAGTAGGAGGAATGCTCATTGCTGCTGGTCCTGTACAGGTGATAGTGGGTAGCTTCATATGGGGTGGTTCAAAGACAAAGAACAAGAAAAAGAAGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

281

Amino Acids

28.54

Weight (kDa)

10.49

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 153 - 267 8.4e-26 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AccB7I CCANNNNNTGG 1 cut(s) 392
AccI GTMKAC 1 cut(s) 183
AciI CCGC 1 cut(s) 379
AclWI GGATC 2 cut(s) 12, 103
AcoI YGGCCR 1 cut(s) 380
AcyI GRCGYC 1 cut(s) 239
AdeI CACNNNGTG 1 cut(s) 248
AfaI GTAC 2 cut(s) 160, 780
AfiI CCNNNNNNNGG 5 cut(s) 150, 193, 342, 368, 392
AgsI TTSAA 2 cut(s) 357, 816
AhdI GACNNNNNGTC 1 cut(s) 582
AjnI CCWGG 3 cut(s) 100, 340, 385
AluBI AGCT 5 cut(s) 353, 443, 536, 798, 841
AluI AGCT 5 cut(s) 353, 443, 536, 798, 841
Alw21I GWGCWC 2 cut(s) 229, 538
Alw26I GTCTC 3 cut(s) 420, 571, 712
Alw44I GTGCAC 1 cut(s) 225
AlwI GGATC 2 cut(s) 12, 103
AlwNI CAGNNNCTG 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 529
AoxI GGCC 3 cut(s) 153, 380, 526
ApaLI GTGCAC 1 cut(s) 225
ApeKI GCWGC 1 cut(s) 767
ArsI GACNNNNNNTTYG 2 cut(s) 682, 714
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 5 cut(s) 13, 153, 290, 527, 773
AsuHPI GGTGA 5 cut(s) 90, 260, 434, 463, 796
AvaI CYCGRG 1 cut(s) 529
AvaII GGWCC 3 cut(s) 13, 290, 773
BaeGI GKGCMC 1 cut(s) 229
BanI GGYRCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 538
BbsI GAAGAC 2 cut(s) 280, 495
Bbv12I GWGCWC 2 cut(s) 229, 538
BbvI GCAGC 1 cut(s) 754
BccI CCATC 4 cut(s) 290, 340, 482, 719
BciT130I CCWGG 3 cut(s) 102, 342, 387
BcoDI GTCTC 3 cut(s) 420, 571, 712
BfaI CTAG 2 cut(s) 435, 717
BfmI CTRYAG 1 cut(s) 842
BfoI RGCGCY 1 cut(s) 242
BisI GCNGC 2 cut(s) 380, 768
BlsI GCNGC 2 cut(s) 381, 769
Bme1390I CCNGG 3 cut(s) 102, 342, 387
Bme18I GGWCC 3 cut(s) 13, 290, 773
BmeRI GACNNNNNGTC 1 cut(s) 582
BmeT110I CYCGRG 1 cut(s) 529
BmgT120I GGNCC 5 cut(s) 13, 153, 290, 527, 773
BmiI GGNNCC 4 cut(s) 14, 154, 240, 679
BmrFI CCNGG 3 cut(s) 102, 342, 387
BmrI ACTGGG 1 cut(s) 75
BmuI ACTGGG 1 cut(s) 75
BpiI GAAGAC 2 cut(s) 280, 495
BplI GAGNNNNNCTC 2 cut(s) 744, 776
BpmI CTGGAG 1 cut(s) 664
BsaHI GRCGYC 1 cut(s) 239
BsaJI CCNNGG 2 cut(s) 341, 386
Bsc4I CCNNNNNNNGG 5 cut(s) 150, 193, 342, 368, 392
Bse1I ACTGG 4 cut(s) 70, 156, 179, 697
Bse3DI GCAATG 4 cut(s) 30, 486, 492, 762
BseBI CCWGG 3 cut(s) 102, 342, 387
BseDI CCNNGG 2 cut(s) 341, 386
BseGI GGATG 3 cut(s) 109, 329, 351
BseLI CCNNNNNNNGG 5 cut(s) 150, 193, 342, 368, 392
BseMI GCAATG 4 cut(s) 30, 486, 492, 762
BseNI ACTGG 4 cut(s) 70, 156, 179, 697
BseRI GAGGAG 1 cut(s) 759
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 1 cut(s) 754
BshFI GGCC 3 cut(s) 155, 382, 528
BshNI GGYRCC 1 cut(s) 238
BsiHKAI GWGCWC 2 cut(s) 229, 538
BsiHKCI CYCGRG 1 cut(s) 529
BslFI GGGAC 3 cut(s) 26, 313, 636
BslI CCNNNNNNNGG 5 cut(s) 150, 193, 342, 368, 392
BsmAI GTCTC 3 cut(s) 420, 571, 712
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 3 cut(s) 26, 313, 636
BsmI GAATGC 2 cut(s) 105, 762
BsnI GGCC 3 cut(s) 155, 382, 528
BsoBI CYCGRG 1 cut(s) 529
Bsp1286I GDGCHC 2 cut(s) 229, 538
Bsp1407I TGTACA 1 cut(s) 778
Bsp143I GATC 3 cut(s) 4, 56, 95
BspACI CCGC 1 cut(s) 379
BspANI GGCC 3 cut(s) 155, 382, 528
BspLI GGNNCC 4 cut(s) 14, 154, 240, 679
BspPI GGATC 2 cut(s) 12, 103
BspT107I GGYRCC 1 cut(s) 238
BsrDI GCAATG 4 cut(s) 30, 486, 492, 762
BsrGI TGTACA 1 cut(s) 778
BsrI ACTGG 4 cut(s) 70, 156, 179, 697
BssECI CCNNGG 2 cut(s) 341, 386
BssMI GATC 3 cut(s) 4, 56, 95
BssNI GRCGYC 1 cut(s) 239
Bst2UI CCWGG 3 cut(s) 102, 342, 387
Bst4CI ACNGT 6 cut(s) 81, 231, 304, 574, 610, 627
Bst6I CTCTTC 1 cut(s) 585
BstACI GRCGYC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 778
BstDEI CTNAG 1 cut(s) 701
BstF5I GGATG 3 cut(s) 109, 329, 351
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 3 cut(s) 7, 59, 98
BstMAI GTCTC 3 cut(s) 420, 571, 712
BstMBI GATC 3 cut(s) 4, 56, 95
BstMWI GCNNNNNNNGC 1 cut(s) 560
BstNI CCWGG 3 cut(s) 102, 342, 387
BstSCI CCNGG 3 cut(s) 100, 340, 385
BstSFI CTRYAG 1 cut(s) 842
BstSLI GKGCMC 1 cut(s) 229
BstV1I GCAGC 1 cut(s) 754
BstV2I GAAGAC 2 cut(s) 280, 495
BstXI CCANNNNNNTGG 1 cut(s) 481
BsuRI GGCC 3 cut(s) 155, 382, 528
BtgZI GCGATG 1 cut(s) 460
BtsCI GGATG 3 cut(s) 109, 329, 351
BtsIMutI CAGTG 1 cut(s) 663
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 5 cut(s) 13, 153, 290, 527, 773
CseI GACGC 2 cut(s) 402, 532
Csp6I GTAC 2 cut(s) 159, 779
CviAII CATG 1 cut(s) 209
CviQI GTAC 2 cut(s) 159, 779
DdeI CTNAG 1 cut(s) 701
DinI GGCGCC 1 cut(s) 240
DpnI GATC 3 cut(s) 6, 58, 97
DpnII GATC 3 cut(s) 4, 56, 95
DraIII CACNNNGTG 1 cut(s) 248
DriI GACNNNNNGTC 1 cut(s) 582
EaeI YGGCCR 1 cut(s) 380
Eam1104I CTCTTC 1 cut(s) 585
Eam1105I GACNNNNNGTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 585
Ecl136II GAGCTC 1 cut(s) 536
Eco24I GRGCYC 1 cut(s) 538
Eco47I GGWCC 3 cut(s) 13, 290, 773
Eco53kI GAGCTC 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 529
EcoICRI GAGCTC 1 cut(s) 536
EcoRII CCWGG 3 cut(s) 100, 340, 385
EcoT38I GRGCYC 1 cut(s) 538
EgeI GGCGCC 1 cut(s) 240
EheI GGCGCC 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 420
FaeI CATG 1 cut(s) 212
FaqI GGGAC 3 cut(s) 26, 313, 636
FatI CATG 1 cut(s) 208
FauNDI CATATG 3 cut(s) 18, 616, 803
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 2 cut(s) 380, 768
FokI GGATG 3 cut(s) 96, 316, 358
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 2 cut(s) 380, 768
FspBI CTAG 2 cut(s) 435, 717
GlaI GCGC 1 cut(s) 240
GluI GCNGC 2 cut(s) 380, 768
GsuI CTGGAG 1 cut(s) 664
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 3 cut(s) 155, 382, 528
HgaI GACGC 2 cut(s) 402, 532
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 239
Hin1II CATG 1 cut(s) 212
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HinfI GANTC 2 cut(s) 251, 577
HphI GGTGA 5 cut(s) 90, 260, 434, 463, 796
Hpy166II GTNNAC 3 cut(s) 171, 184, 227
Hpy188I TCNGA 2 cut(s) 9, 54
Hpy188III TCNNGA 3 cut(s) 72, 681, 722
Hpy8I GTNNAC 3 cut(s) 171, 184, 227
HpyAV CCTTC 1 cut(s) 198
HpyCH4III ACNGT 6 cut(s) 81, 231, 304, 574, 610, 627
HpyCH4V TGCA 3 cut(s) 131, 227, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 560
HpyF3I CTNAG 1 cut(s) 701
Hsp92I GRCGYC 1 cut(s) 239
Hsp92II CATG 1 cut(s) 212
HspAI GCGC 1 cut(s) 239
KasI GGCGCC 1 cut(s) 238
Kzo9I GATC 3 cut(s) 4, 56, 95
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 1 cut(s) 754
MaeI CTAG 2 cut(s) 435, 717
MaeIII GTNAC 3 cut(s) 87, 574, 610
MalI GATC 3 cut(s) 6, 58, 97
MboI GATC 3 cut(s) 4, 56, 95
MboII GAAGA 5 cut(s) 183, 285, 409, 500, 572
MhlI GDGCHC 2 cut(s) 229, 538
MluCI AATT 2 cut(s) 407, 636
Mly113I GGCGCC 1 cut(s) 239
MlyI GAGTC 1 cut(s) 571
MslI CAYNNNNRTG 1 cut(s) 479
MspA1I CMGCKG 1 cut(s) 443
MspR9I CCNGG 3 cut(s) 102, 342, 387
Mva1269I GAATGC 2 cut(s) 105, 762
MvaI CCWGG 3 cut(s) 102, 342, 387
MwoI GCNNNNNNNGC 1 cut(s) 560
NarI GGCGCC 1 cut(s) 239
NdeI CATATG 3 cut(s) 18, 616, 803
NdeII GATC 3 cut(s) 4, 56, 95
NlaIII CATG 1 cut(s) 212
NlaIV GGNNCC 4 cut(s) 14, 154, 240, 679
NmuCI GTSAC 2 cut(s) 574, 610
PctI GAATGC 2 cut(s) 105, 762
PfeI GAWTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 2 cut(s) 381, 769
PleI GAGTC 1 cut(s) 571
PluTI GGCGCC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 571
Psp124BI GAGCTC 1 cut(s) 538
Psp6I CCWGG 3 cut(s) 100, 340, 385
PspGI CCWGG 3 cut(s) 100, 340, 385
PspN4I GGNNCC 4 cut(s) 14, 154, 240, 679
PspPI GGNCC 5 cut(s) 13, 153, 290, 527, 773
PstNI CAGNNNCTG 1 cut(s) 392
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 2 cut(s) 160, 780
RsaNI GTAC 2 cut(s) 159, 779
RseI CAYNNNNRTG 1 cut(s) 479
SacI GAGCTC 1 cut(s) 538
SatI GCNGC 2 cut(s) 380, 768
Sau3AI GATC 3 cut(s) 4, 56, 95
Sau96I GGNCC 5 cut(s) 13, 153, 290, 527, 773
SchI GAGTC 1 cut(s) 571
ScrFI CCNGG 3 cut(s) 102, 342, 387
SduI GDGCHC 2 cut(s) 229, 538
SfcI CTRYAG 1 cut(s) 842
SfoI GGCGCC 1 cut(s) 240
SinI GGWCC 3 cut(s) 13, 290, 773
SmiMI CAYNNNNRTG 1 cut(s) 479
Sse9I AATT 2 cut(s) 407, 636
SsiI CCGC 1 cut(s) 379
SspDI GGCGCC 1 cut(s) 238
SspMI CTAG 2 cut(s) 435, 717
SstI GAGCTC 1 cut(s) 538
StyD4I CCNGG 3 cut(s) 100, 340, 385
TaaI ACNGT 6 cut(s) 81, 231, 304, 574, 610, 627
TaqI TCGA 2 cut(s) 280, 688
TasI AATT 2 cut(s) 407, 636
TatI WGTACW 1 cut(s) 778
TauI GCSGC 1 cut(s) 382
TfiI GAWTC 1 cut(s) 251
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 2 cut(s) 574, 610
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 2 cut(s) 574, 610
TspDTI ATGAA 4 cut(s) 98, 197, 297, 790
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 1 cut(s) 670
Van91I CCANNNNNTGG 1 cut(s) 392
VneI GTGCAC 1 cut(s) 225
VpaK11BI GGWCC 3 cut(s) 13, 290, 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 2 cut(s) 435, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.